CN102247417B - Fat reduction effect of total saponins and polysaccharides of Panax japonicus - Google Patents
Fat reduction effect of total saponins and polysaccharides of Panax japonicus Download PDFInfo
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- CN102247417B CN102247417B CN 201010176646 CN201010176646A CN102247417B CN 102247417 B CN102247417 B CN 102247417B CN 201010176646 CN201010176646 CN 201010176646 CN 201010176646 A CN201010176646 A CN 201010176646A CN 102247417 B CN102247417 B CN 102247417B
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Abstract
The invention relates to a fat reduction purpose of total saponins and polysaccharides of Panax japonicus, and compositions of the total saponins and the polysaccharides of Panax japonicus. The total saponins and the polysaccharides of Panax japonicus can be mixed with pharmaceutically acceptable carriers.
Description
Technical field
The fat-reducing purposes that relates to panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides of the present invention.
Background technology
Obesity mainly is divided into two kinds of Simple Obesity and morbid oberities.Simple Obesity mainly is to be caused by hyperalimentation, and the substance in vivo metabolism is slower, thus the generation that the synthetic speed of material causes fat greater than the speed of decomposing.Morbid oberity mainly causes by disease, for example Ke's property syndrome and hypothyroidism etc.Fat concrete pathogenesis is various, but has all influenced patient's quality of life largely, and serious obesity more can cause the generation of a series of complication.
Yet at the treatment of obesity, now chemicals adopts Fenfluramine more, and it has strong drug dependence and addiction, and with slight stomachache, side effect such as dizzy, weak, drowsiness.And Chinese medicine is taked the compound recipe decoction to the treatment of obesity more, or is derived from the Chinese patent medicine of compound recipe, and its flavour of a drug are numerous, causes difficult quality control.Therefore, need develop in a hurry and come from single plant, composition is simple, and the effective site that curative effect is clear and definite or effective ingredient are used for fat treatment.
Rhizoma Panacis Japonici is the dry root of Araliaceae Panax herbaceos perennial Panax japonicus C1 A1 Mey1.Former is a kind of famous and precious Chinese herbal medicine among the people, has now recorded in the Pharmacopoeia of the People's Republic of China the similar Radix Ginseng of medicinal function and Radix Notoginseng.Rhizoma Panacis Japonici is born in by hillside limes marginis, dark and damp area or the rock ditch ravine.Mainly be distributed in ground such as Guizhou, Hunan, Yunnan, Anhui, Jiangxi, Hubei.Excavate annual autumn, removes stem and leaf, tells fleshy tap root, and rhizome is removed crust, dries or dry in the shade and can be used as medicine.Beginning is stated from supplementary Amplifications of the Compendium of Materia Medica.Sweet, the little hardship of Rhizoma Panacis Japonici, temperature.Have multiple efficacies such as blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, strengthening by means of tonics.The strengthening by means of tonics effect of existing similar Radix Ginseng has eliminating stasis to stop pain, hemostasis, the phlegm-dispelling functions of similar Radix Notoginseng again, be mainly used in weak after being ill, chronic cough hemoptysis, cough with copious phlegm, traumatic injury.Rhizoma Panacis Japonici contains multiple saponin, volatile oil, saccharide and several amino acids.
The technical problem to be solved in the present invention is the fat-reducing purposes of research panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides.For addressing the above problem, provide technical scheme as follows.
Panax japonicus total saponins, the application of Rhizoma Panacis Japonici polysaccharides in fat-reducing.
The utilization of described fat-reducing aspect comprises prevention and the treatment to obesity.
Described compositions comprises panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides and pharmaceutically acceptable carrier or conventional edible adjuvant.
The present invention all has significance reduction effect to the body weight of laboratory animal.The present invention includes with panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides and mix with pharmaceutically acceptable carrier.
Pharmaceutically acceptable carrier comprises the adjuvant of oral formulations adjuvant or parenteral administration.Route of administration can be oral, injection, topical etc.According to technical scheme of the present invention, said composition can be oral formulations or injection preparation, and wherein oral formulations comprises capsule, soft capsule, granule, oral liquid, tablet, drop pill etc.Used adjuvant comprises: conventional adjuvants such as starch, sucrose, lactose, Icing Sugar, glucose, mannitol, xylitol, Polyethylene Glycol, isopropyl alcohol, tween 80, glycerol, propylene glycol, microcrystalline Cellulose sodium, dextrin, cyclodextrin, sodium chloride, vitamin C, cysteine, citric acid, sodium thiosulfate, sodium sulfite, stearate and gelatin, preparation later stage preparation technology and equipment all belong to the routine techniques of pharmaceutical field, the present invention does not limit this, so will not describe in detail at this.
The dosage of present composition oral administration is 2-3 time/day, 0.25～0.50g/ time.
Panax japonicus total saponins of the present invention, Rhizoma Panacis Japonici polysaccharides extract are to extract to obtain from natural Chinese medicine Rhizoma Panacis Japonici, and Rhizoma Panacis Japonici is the Chinese herbal medicine of using always, and long-term traditional Chinese medical science medication experience is arranged, and this medicine is safe to use, no overt toxicity effect record.
Panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides have the effect of definite reduction laboratory animal body weight.Therefore, panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides have the prospect that is developed to slimming medicine.
Extraction, purification and the preparation of embodiment one panax japonicus total saponins
Get the Rhizoma Panacis Japonici coarse powder, add 60% ethanol of 10 times of amounts, soaked 2 hours, water-bath reflux, extract, 2 hours, filtration gets filtrate.Medicinal residues are used 60% the alcohol reflux 2 times of 10 times of amounts again, each 2 hours, filter.Merge 3 times filtrate, decompression recycling ethanol is not to there being the alcohol flavor, and be mixed with medical material: medicinal liquid (g/ml) is 1: 1 solution.On the HPD-100 macroporous resin column handled well, water, 60% ethanol elution.Merge 60% ethanol elution, reclaim solvent and do near, through 65 ℃ of drying under reduced pressure, obtain panax japonicus total saponins.
Extraction, purification and the preparation of embodiment two Rhizoma Panacis Japonici polysaccharidess
Get the Rhizoma Panacis Japonici coarse powder, add the water of 10 times of amounts, soaked 2 hours, straight fire decocts and extracted 1.5 hours, filters, and gets filtrate.Medicinal residues decoct extraction 2 times with the straight fire of water of 10 times of amounts again, each 1.5 hours, filter.Merge 3 times filtrate, it is an amount of to be evaporated to volume.Add ethanol to concentration and be 70% and carry out precipitate with ethanol, place under the room temperature more than 12 hours, filter, the collecting precipitation thing, precipitate with ethanol is 2 times repeatedly, merges precipitate, through 65 ℃ of drying under reduced pressure, obtains Rhizoma Panacis Japonici polysaccharides.
The weight-reducing experiment research of embodiment three panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides
1. experiment material
1.1 medicine and reagent
Rhizoma Panacis Japonici polysaccharides (D1), panax japonicus total saponins (Z1) are the self-control of this laboratory.
1.2 experimental apparatus
Satrious electronic balance (Germany).
1.3 laboratory animal
Kunming kind male white mouse, body weight 18～22g, China Medicine University's animal center provides.
2. the preparation of laboratory sample
Panax japonicus total saponins, the Rhizoma Panacis Japonici polysaccharides of preparation among the embodiment one, two.
3. the preparation of test specimen liquid
3.1.CMC-Na the preparation of solution: precision takes by weighing 5gCMC-Na, the 1000mL distilled water is mixed with concentration and is 0.5% CMC-Na solution.
3.2. the preparation of high dose Rhizoma Panacis Japonici polysaccharides medicinal liquid: precision takes by weighing the 1.5g Rhizoma Panacis Japonici polysaccharides.100ml 0.5%CMC-Na suspendible, being mixed with concentration is the medicinal liquid sample of 0.015g/ml.
3.3. the preparation of low dosage Rhizoma Panacis Japonici polysaccharides medicinal liquid: precision takes by weighing the 0.75g Rhizoma Panacis Japonici polysaccharides, the 100ml0.5%CMC-Na suspendible, and being mixed with concentration is the medicinal liquid sample of 0.0075g/ml.
3.4. the preparation of high dose panax japonicus total saponins medicinal liquid: precision takes by weighing the 1.Sg panax japonicus total saponins.100ml 0.5%CMC-Na suspendible, being mixed with concentration is the medicinal liquid sample of 0.015g/ml.
3.5. the preparation of low dosage panax japonicus total saponins medicinal liquid: precision takes by weighing the 0.75g panax japonicus total saponins, the 100ml0.5%CMC-Na suspendible, and being mixed with concentration is the medicinal liquid sample of 0.0075g/ml.
3.6. the preparation of high dose panax japonicus total saponins and polysaccharide medicinal liquid: precision takes by weighing 0.75g panax japonicus total saponins and 0.75g Rhizoma Panacis Japonici polysaccharides respectively.100ml 0.5%CMC-Na suspendible, being mixed with concentration is the medicinal liquid sample of 0.015g/ml.
3.7. the preparation of low dosage panax japonicus total saponins medicinal liquid: precision takes by weighing 0.375g panax japonicus total saponins and 0.375g Rhizoma Panacis Japonici polysaccharides respectively.The 100ml0.5%CMC-Na suspendible, being mixed with concentration is the medicinal liquid sample of 0.0075g/ml.
4. experimental technique
70 of Kunming mouses, 18-22g, male and female half and half are divided into 7 groups at random, 10 every group.Be divided into normal blank group, panax japonicus total saponins (Z1) high dose group, panax japonicus total saponins (Z1) low dose group, Rhizoma Panacis Japonici polysaccharides (D1) high dose group, Rhizoma Panacis Japonici polysaccharides (D1) low dose group, Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins low dose group (Z1D1), Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins high dose group (Z1D1).Gastric infusion.The normal control group is pressed 0.2ml/10g every day and is irritated stomach 0.5%CMCNa, the administration group is irritated the relative medicine that stomach gives the 0.2ml/10g volume every day, and dosage is respectively: the Rhizoma Panacis Japonici polysaccharides high dose group is that 0.3g/kg, Rhizoma Panacis Japonici polysaccharides low dose group are that 0.15g/kg, panax japonicus total saponins high dose group are that 0.3g/kg, panax japonicus total saponins low dose group are that 0.15/kg, Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins high dose group are that 0.15g polysaccharide/kg and 0.15g saponin/kg, Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins low dose group are 0.075g polysaccharide/kg and 0.075g saponin/kg.Administration is 14 days altogether.Body weight with the 15th natural gift another name amount laboratory animal the results are shown in following table.
Table 1. mice body weight determination result (n=10,
Unit of weight: g)
Annotate:
*Expression administration group has been compared utmost point significant difference with blank.
5. result and discussion
Experimental result shows, compare with normal group, the body weight of panax japonicus total saponins (Z1) high dose group, panax japonicus total saponins (Z1) low dose group, Rhizoma Panacis Japonici polysaccharides (D1) high dose group, Rhizoma Panacis Japonici polysaccharides (D1) low dose group, Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins (Z1D1) low dose group, Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins (Z1D1) high dose group experiment mice all has significance to reduce variation, P＜0.01.Show that panax japonicus total saponins, Rhizoma Panacis Japonici polysaccharides all have the antiobesity action that lowers the laboratory animal body weight.
Claims (4)
1. the compositions of Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins or panax japonicus total saponins are in the purposes of preparation in the slimming medicine, and described panax japonicus total saponins adopts following method preparation:
Get the Rhizoma Panacis Japonici coarse powder, 60% the ethanol that adds 10 times of amounts, soaked 2 hours, water-bath reflux, extract, 2 hours filters, and gets filtrate, medicinal residues are used 60% the alcohol reflux 2 times of 10 times of amounts again, each 2 hours, filter, merge 3 times filtrate, decompression recycling ethanol is to there not being the alcohol flavor, be mixed with medical material: medicinal liquid g/ml is 1: 1 solution, on the HPD-100 macroporous resin column handled well, water, 60% ethanol elution, merge 60% ethanol elution, reclaim solvent and do near, through 65 ℃ of drying under reduced pressure, obtain panax japonicus total saponins;
Described Rhizoma Panacis Japonici polysaccharides adopts following method preparation:
Get the Rhizoma Panacis Japonici coarse powder, add the water of 10 times of amounts, soaked 2 hours, straight fire decocts and extracted 1.5 hours, filter, get filtrate, medicinal residues decoct extraction 2 times with the straight fire of water of 10 times of amounts again, each 1.5 hours, filter, merge 3 times filtrate, it is an amount of to be evaporated to volume, adds ethanol to concentration and is 70% and carry out precipitate with ethanol, places more than 12 hours under the room temperature, filter, the collecting precipitation thing, precipitate with ethanol is 2 times repeatedly, merge precipitate, through 65 ℃ of drying under reduced pressure, obtain Rhizoma Panacis Japonici polysaccharides.
2. according to the purposes described in the claim 1, it is characterized in that described fat-reducing mainly is that compositions or panax japonicus total saponins by Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins reaches reducing the body weight effect.
3. purposes according to claim 1 is characterized in that compositions or the application of panax japonicus total saponins in preparation prevention and treatment of obesity medicine of Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins.
4. according to the purposes described in the claim 1, it is characterized in that described medicine comprises compositions or panax japonicus total saponins and pharmaceutically acceptable carrier or the conventional edible adjuvant of Rhizoma Panacis Japonici polysaccharides and panax japonicus total saponins.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101249121A (en) * | 2008-03-24 | 2008-08-27 | 三峡大学 | Panax japonicus total saponins preparation and its applications in medicament for curing myocardial ischemia |
| CN101265301A (en) * | 2008-04-29 | 2008-09-17 | 武汉大学 | Panax japonicus polysaccharides, preparation method and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101249121A (en) * | 2008-03-24 | 2008-08-27 | 三峡大学 | Panax japonicus total saponins preparation and its applications in medicament for curing myocardial ischemia |
| CN101265301A (en) * | 2008-04-29 | 2008-09-17 | 武汉大学 | Panax japonicus polysaccharides, preparation method and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘桂林等.鄂产竹节参多糖成分抗疲劳作用的研究.《中国医院药学杂志》.2006,第26卷(第12期), |
| 鄂产竹节参多糖成分抗疲劳作用的研究;刘桂林等;《中国医院药学杂志》;20061228;第26卷(第12期);1459-1461 * |
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