CN102234666A - 赖氨酸的流加发酵制备 - Google Patents
赖氨酸的流加发酵制备 Download PDFInfo
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- CN102234666A CN102234666A CN 201110151495 CN201110151495A CN102234666A CN 102234666 A CN102234666 A CN 102234666A CN 201110151495 CN201110151495 CN 201110151495 CN 201110151495 A CN201110151495 A CN 201110151495A CN 102234666 A CN102234666 A CN 102234666A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims description 5
- 239000004472 Lysine Substances 0.000 title description 5
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title description 4
- 102000000818 NADP Transhydrogenases Human genes 0.000 claims abstract description 37
- 108010001609 NADP Transhydrogenases Proteins 0.000 claims abstract description 37
- 238000000034 method Methods 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims abstract description 29
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 20
- 239000008103 glucose Substances 0.000 claims description 20
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 17
- 229930195722 L-methionine Natural products 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 17
- 241000186031 Corynebacteriaceae Species 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 11
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- 239000011724 folic acid Substances 0.000 claims description 11
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 10
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- 238000009923 sugaring Methods 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
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- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 description 13
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- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 4
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- 239000002609 medium Substances 0.000 description 4
- 229930182817 methionine Natural products 0.000 description 4
- 229920006395 saturated elastomer Polymers 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
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- 230000008676 import Effects 0.000 description 3
- 235000018977 lysine Nutrition 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 150000007523 nucleic acids Chemical group 0.000 description 3
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- 238000004809 thin layer chromatography Methods 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
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- 108010061982 DNA Ligases Proteins 0.000 description 2
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- 238000012408 PCR amplification Methods 0.000 description 2
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- 238000010369 molecular cloning Methods 0.000 description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000186226 Corynebacterium glutamicum Species 0.000 description 1
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- 235000019766 L-Lysine Nutrition 0.000 description 1
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- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533889A (zh) * | 2012-01-13 | 2012-07-04 | 中粮生物化学(安徽)股份有限公司 | 一种赖氨酸连续发酵的方法 |
CN103444984A (zh) * | 2012-06-07 | 2013-12-18 | 宁夏伊品生物科技股份有限公司 | 赖氨酸、木聚糖酶的发酵及包被饲料 |
CN104278062A (zh) * | 2013-07-01 | 2015-01-14 | 中粮生物化学(安徽)股份有限公司 | 处理糖蜜的方法及得到的糖蜜清液以及赖氨酸发酵培养基及赖氨酸的生产方法 |
CN104726512A (zh) * | 2013-12-24 | 2015-06-24 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN104726510A (zh) * | 2013-12-24 | 2015-06-24 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN105755065A (zh) * | 2014-12-17 | 2016-07-13 | 宁夏伊品生物科技股份有限公司 | 赖氨酸的发酵及其中所用的设备和部件 |
CN105779523A (zh) * | 2014-12-17 | 2016-07-20 | 宁夏伊品生物科技股份有限公司 | 流加发酵及其设备 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1139956A (zh) * | 1993-10-28 | 1997-01-08 | 味之素株式会社 | 物质的生产方法 |
CN101104863A (zh) * | 2007-05-29 | 2008-01-16 | 宁夏伊品生物工程有限公司 | 赖氨酸发酵的方法 |
-
2011
- 2011-06-08 CN CN2011101514954A patent/CN102234666B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1139956A (zh) * | 1993-10-28 | 1997-01-08 | 味之素株式会社 | 物质的生产方法 |
CN101104863A (zh) * | 2007-05-29 | 2008-01-16 | 宁夏伊品生物工程有限公司 | 赖氨酸发酵的方法 |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533889B (zh) * | 2012-01-13 | 2014-07-16 | 中粮生物化学(安徽)股份有限公司 | 一种赖氨酸连续发酵的方法 |
CN102533889A (zh) * | 2012-01-13 | 2012-07-04 | 中粮生物化学(安徽)股份有限公司 | 一种赖氨酸连续发酵的方法 |
CN103444984B (zh) * | 2012-06-07 | 2015-09-09 | 宁夏伊品生物科技股份有限公司 | 赖氨酸、木聚糖酶的发酵及包被饲料 |
CN103444984A (zh) * | 2012-06-07 | 2013-12-18 | 宁夏伊品生物科技股份有限公司 | 赖氨酸、木聚糖酶的发酵及包被饲料 |
CN104278062A (zh) * | 2013-07-01 | 2015-01-14 | 中粮生物化学(安徽)股份有限公司 | 处理糖蜜的方法及得到的糖蜜清液以及赖氨酸发酵培养基及赖氨酸的生产方法 |
CN104726510A (zh) * | 2013-12-24 | 2015-06-24 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN104726512A (zh) * | 2013-12-24 | 2015-06-24 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN104726510B (zh) * | 2013-12-24 | 2018-07-17 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN104726512B (zh) * | 2013-12-24 | 2018-08-07 | 中粮生物化学(安徽)股份有限公司 | 一种发酵制备赖氨酸的方法 |
CN105755065A (zh) * | 2014-12-17 | 2016-07-13 | 宁夏伊品生物科技股份有限公司 | 赖氨酸的发酵及其中所用的设备和部件 |
CN105779523A (zh) * | 2014-12-17 | 2016-07-20 | 宁夏伊品生物科技股份有限公司 | 流加发酵及其设备 |
CN105755065B (zh) * | 2014-12-17 | 2019-01-08 | 内蒙古伊品生物科技有限公司 | 赖氨酸的发酵及其中所用的设备和部件 |
CN105779523B (zh) * | 2014-12-17 | 2019-01-08 | 内蒙古伊品生物科技有限公司 | 流加发酵及其设备 |
CN109370882A (zh) * | 2014-12-17 | 2019-02-22 | 宁夏伊品生物科技股份有限公司 | 赖氨酸的发酵中所用的设备和部件 |
CN109370882B (zh) * | 2014-12-17 | 2021-08-20 | 宁夏伊品生物科技股份有限公司 | 赖氨酸的发酵中所用的设备和部件 |
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Denomination of invention: Fed-batch fermentation preparation of lysine Effective date of registration: 20200120 Granted publication date: 20120815 Pledgee: Yongning County Branch of China Agricultural Development Bank Pledgor: NINGXIA EPPEN BIOTECH Co.,Ltd.|INNER MONGOLIA EPPEN BIOTECH Co.,Ltd. Registration number: Y2020640000002 |
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