CN102234615A - High-efficiency combined functional bacteria for sludge treatment - Google Patents
High-efficiency combined functional bacteria for sludge treatment Download PDFInfo
- Publication number
- CN102234615A CN102234615A CN2010101535496A CN201010153549A CN102234615A CN 102234615 A CN102234615 A CN 102234615A CN 2010101535496 A CN2010101535496 A CN 2010101535496A CN 201010153549 A CN201010153549 A CN 201010153549A CN 102234615 A CN102234615 A CN 102234615A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- sludge treatment
- sludge
- substratum
- combination function
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses high-efficiency combined functional bacteria for sludge treatment. The high-efficiency combined functional bacteria comprise the following six microbes: bacillus licheniformis, bacillus subtilis, bacillus lentus, bacillus megaterium, bacillus thuringiensis and bacillus cereus. A culture method for the microbes comprises the following steps of: sampling; culturing in a culture medium; separating target strains; performing index analysis on the separated strains; identifying species; mixing; and optimizing. The functional bacteria are quick in multiplication and have obvious effect of removing phosphorus from sludge in the process of decomposing organic matters, so that the sludge treatment effect ensures that the sludge reaches the national emission standard, and the sludge discharge can be greatly reduced.
Description
[technical field]
The present invention relates to the efficient combination function bacterium of a kind of sludge treatment.
[background technology]
Microorganism is the same with animals and plants, be one of nature three big species, and in the whole ecological recycle system, microorganism is the decomposer of plant and animal residues organic waste.Therefore, microorganism has played the part of a crucial role, the i.e. processor of mud and rubbish in ecological circulation and environmental protection field.Nearly all sludge treatment is all finished by action of microorganisms, and in mud and dirt processing, not only needs microbiological degradation and remove various objectionable impuritiess, also will carry out deodorizing by microorganism.And kind of microorganism and function and quantity have determined the processing speed and the treatment effect of mud and dirt to a great extent.
Traditional method for sludge treatment is technologies such as precipitation, anaerobism, aerobic aeration, and used function yeast is taken from natural active sludge, promptly by degrade organic waste in the mud of the natural anaerobism of nature, aerobic microbiological.The water outlet effect of traditional sludge treatment process usually is subjected to the influence of environmental factors (weather, season, mud different sources etc.) and seems unstable, and the same problem that nearly all sludge treatment factory faces is exactly post-depositional sludge disposal and the secondary pollution problem brought.In addition, the processing of some special source mud is still needed and will be taked special chemical means, and this pollution for environment is more serious.
In view of the above problems, be necessary to cultivate the efficient combination function bacterium of a kind of sludge treatment and solve the problems referred to above.
[summary of the invention]
Technical problem solved by the invention is to provide a kind of sludge treatment efficient combination function bacterium, and it can effectively decompose the objectionable impurities in the mud, reduces sludge discharge.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: the efficient combination function bacterium of a kind of sludge treatment, wherein, it comprises following six kinds of microorganisms: Bacillus licheniformis, subtilis, bacillus lentus, bacillus megaterium, bacillus thuringiensis, bacillus cereus, the cultivation of described microorganism are via following process: cultivate in sampling, the substratum, the separation of purpose bacterial classification, isolated bacterial classification done index analysis, kind evaluation, compatibility and optimization.
Further, contain peptone 35 grams, glucose 4 grams, sodium-chlor 3 grams in described per 1000 milliliters substratum.
When utilizing described culture medium culturing, as solvent, and, under the air pressure conditions of 1.034 * 100000Pa, substratum was carried out sterilising treatment 20 minutes with the pH value to 7.2 that NaOH regulates substratum with distilled water.
Described function yeast to erythromycin, norfloxicin and Rifampin medium sensitivity, has resistance to tsiklomitsin, mydecamycin and cefuroxime to lomefloxacin, Ciprofloxacin and penicillin sensitivity.
Compared to prior art, the efficient combination function bacterium of sludge treatment of the present invention can make the treatment effect of mud reach discharging standards, significantly reduces sludge discharge, realizes ecology, minimizing that urban industry and domestic sludge are administered, disposal of resources.
[description of drawings]
Fig. 1 is the ultimate principle figure that mud ecology of the present invention is handled.
Fig. 2 is that organism of the present invention decomposes the approach synoptic diagram.
Fig. 3 is screening and the seed selection schema of the efficient combination function bacterium of sludge treatment of the present invention.
[embodiment]
The efficient combination function bacterium of sludge treatment of the present invention is mainly used in the mud ecology and handles, and the ultimate principle that its ecology is handled sees also Figure 1 and Figure 2.Its main component of biological substance is polysaccharide, protein and fat etc.Under the nature situation, the microorganism in these materials dependence environment etc. is slowly decomposed their to keep the balance of whole ecological environment.The mud ecology is handled and promptly to be utilized this ultimate principle, and isolating from occurring in nature a collection ofly has the microorganism species of specific degradation capability to organic waste the mud, is combined into one group of combination function bacterium after isolation identification.Under suitable condition (temperature, humidity and air etc.), these special combination floras can produce proteolytic enzyme effectively, lipase, lytic enzyme, cellulase, amylase etc., polymer substance is resolved into can be by the lower-molecular substance of microbiological degradation, and being translated into carbonic acid gas, water, alcohol, organic acid and little ammonia, the form with gas and water discharges at last.In brief, be exactly that some special bacteriums can be resolved into free of contamination carbonic acid gas and water to organic waste in the mud (and sedimentary mud); Organonitrogen becomes NH4 through the ammonifying bacteria ammonification, forms nitrate through nitrated mattress oxidation, under anaerobic carries out denitrogenation through denitrifying bacteria again.Mentioned microorganism biochemical degradation process makes the processing of mud reach the target of minimizing, harmless ecology.
See also shown in Figure 3ly, be the screening and the seed selection process of the efficient combination function bacterial classification of sludge treatment of the present invention, it comprises the separation of bacterial classification and the screening and the compatibility of efficient function yeast bacterial classification.
Need satisfy following condition in the sepn process of described bacterial classification: at first, the source of used bacterial classification: handle the employed microorganism combination function of municipal sludge bacterium and all separate, screen, cultivate and optimize and form, do not do any hereditary mutagenesis and genetic modification work by occurring in nature.Secondly, used substratum: beef-protein medium and through the substratum of particular design, wherein, contain peptone 35 grams, glucose 4 grams, sodium-chlor 3 grams in described per 1000 milliliters of substratum, under the condition of laboratory configuration, as solvent, regulate pH value to 7.2 with NaOH with distilled water, under the air pressure conditions of 1.034 * 100000Pa, sterilized 20 minutes.Once more, the separation of bacterial classification: on the basis of gathering all kinds of bacterial classification samples comparatively widely, carry out the inclined-plane inoculation, the required bacterial strain that is separated to after the sample of gathering increased rich the cultivation." at correspondent method (hydrolysis method) " and methods such as " shotguns " are made Preliminary detection, again through the substratum of particular design separate, screening, obtain a large amount of bacterial strains, collect wherein to possess and meet bacterial strain that purpose requires as test strain.
Screening of described efficient combination function bacterium and compatibility are the processes of a complexity, need the years input just can obtain good purpose bacterial classification combination usually.Therefore, in sludge treatment technique, when described active function bacteria strain is screened, employing helps the functional microorganism public facility of growth fast, with the index as the efficient processing of screening bacterial classification such as mud COD lowering speed, decline index and deodorizing effect, thereby obtained required bacterial strain faster.The Screening Treatment process comprises that each single strain degradation rate is measured and the bacterium test is respectively made up in allotment.
According to each single strain test of many times result in miniature biochemical handler and small-sized AOS system, compatibility becomes the combination bacterium, carries out dynamic pilot scale.
Finally, in conjunction with dynamically pilot scale, be purpose with the significantly minimizing and the deodorizing effect of water outlet COD value, sludge discharge, the proportioning through combination, the many bacterial classifications of optimization finally obtains one group " the efficient combination function bacterium of sludge treatment ".Filter out efficient combination function flora, its corresponding decomposition and inversion function key problem in technology is: comprise kind (genus bacillus, Bacillus subtilus etc.), genetic stability (through continuous passage cultivation and simultaneous test, the taxonomic character of all bacterium and stable to the maintenances such as susceptibility physicochemical character of medicine), resistance (refers to the sensitivity test to more than 50 kinds of microbiotic medicines, as to Alcaligenes faecalis, lomefloxacin, Ciprofloxacin, penicillin etc. responsive [s]; Medium sensitivities such as erythromycin, norfloxicin, Rifampin [M] had resistance [R] and biological safety (to the individual, colony is healthy and ecotope is safe from harm) to tsiklomitsin, mydecamycin, cefuroxime etc.
What pay special attention to is that the efficient combination function bacterium of described sludge treatment comprises following six kinds of microorganisms: Bacillus licheniformis, subtilis, bacillus lentus, bacillus megaterium, bacillus thuringiensis, bacillus cereus.In order to obtain ideal function yeast concentration, on Bechtop in above-mentioned six kinds of microbial strains seed to the 10 milliliter test tubes of picking, temperature condition is 37 ℃, and at thermal agitation under the rotating speed of 300rpm more than 8 hours, amplification culture volume step by step later on reaches 5 * 1000000000 cells/ml to the concentration of final function yeast.
In described sludge handling process, described efficient combination function bacterium is adsorbed on the carrier that is provided with in the sludge treatment pond, and through constantly coming off and adsorption process, has formed countless aerobic zones and anaerobic zone in the sludge treatment pond.By anoxic, aerobic processes, described efficient combination function bacterium carries out nitrated, denitrification in degradation of organic substances, make the mud after above-mentioned processing enter settling tank again.
The efficient combination function bacterium that the present invention relates to breeds in the decomposing organic matter process soon, and the organism in the 70-80% water body is used for synthesizing self cell tissue, therefore the nutritive substance in the water body is required high, particularly obvious to the assimilation effect of nitrogen and phosphorus.Because described function yeast self is active strong, need to consume a large amount of ATP (amount of predetermined substance can reflect microbic activity in the Triphosaden, microbe), and phosphorus is the important composition element of ATP in the predetermined substance simultaneously.Therefore, the consumption of phosphorus is big in the water body, and phosphor-removing effect is obvious.
The efficient combination function bacterium that the present invention adopts, make the treatment effect of mud reach discharging standards, significantly reduce sludge discharge, ecology, minimizing that urban industry and domestic sludge are administered have been realized, disposal of resources, and the efficient combination function bacterium that the present invention adopts all has the function of efficient conversion degraded organic waste.
The above only is a most preferred embodiment of the present invention, is not the present invention is done any pro forma restriction.Any those of ordinary skill in the art are not breaking away under the technical solution of the present invention scope situation, utilize the method content of above-mentioned announcement that technical solution of the present invention is made many possible changes and modification, all belong to the scope of claims protection.
Claims (4)
1. efficient combination function bacterium of sludge treatment, it is characterized in that: it comprises following six kinds of microorganisms: Bacillus licheniformis, subtilis, bacillus lentus, bacillus megaterium, bacillus thuringiensis, bacillus cereus, the cultivation of described microorganism are via following process: cultivate in sampling, the substratum, the separation of purpose bacterial classification, isolated bacterial classification done index analysis, kind evaluation, compatibility and optimization.
2. the efficient combination function bacterium of sludge treatment as claimed in claim 1 is characterized in that: contain peptone 35 grams, glucose 4 grams, sodium-chlor 3 grams in described per 1000 milliliters substratum.
3. the efficient combination function bacterium of sludge treatment as claimed in claim 2, it is characterized in that: when utilizing described culture medium culturing, with distilled water as solvent, and the pH value to 7.2 of regulating substratum with NaOH, under the air pressure conditions of 1.034 * 100000Pa, substratum was carried out sterilising treatment 20 minutes.
4. the efficient combination function bacterium of sludge treatment as claimed in claim 3, it is characterized in that: described function yeast is to lomefloxacin, Ciprofloxacin and penicillin sensitivity, to erythromycin, norfloxicin and Rifampin medium sensitivity, tsiklomitsin, mydecamycin and cefuroxime had resistance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101535496A CN102234615A (en) | 2010-04-22 | 2010-04-22 | High-efficiency combined functional bacteria for sludge treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101535496A CN102234615A (en) | 2010-04-22 | 2010-04-22 | High-efficiency combined functional bacteria for sludge treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102234615A true CN102234615A (en) | 2011-11-09 |
Family
ID=44885744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101535496A Pending CN102234615A (en) | 2010-04-22 | 2010-04-22 | High-efficiency combined functional bacteria for sludge treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102234615A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102583925A (en) * | 2012-02-03 | 2012-07-18 | 哈尔滨工业大学 | Method for pretreating excess sludge by adopting bio-augmentation technology |
| CN104805032A (en) * | 2014-12-29 | 2015-07-29 | 天津北洋百川生物技术有限公司 | Multifunctional microbial agent for sludge reduction and preparation thereof |
| CN107032574A (en) * | 2017-06-09 | 2017-08-11 | 杜文洁 | Municipal sludge processing composite bacteria agent and preparation method thereof |
| CN107162175A (en) * | 2017-05-12 | 2017-09-15 | 南京大学 | A kind of method that training degraded penicillin activated sludge is tamed and dociled by co-substrate of glucose |
| CN107312725A (en) * | 2017-05-26 | 2017-11-03 | 复环生物科技(上海)有限公司 | One group of functional microorganism flora for promoting to form high-quality ecological fertilizer |
-
2010
- 2010-04-22 CN CN2010101535496A patent/CN102234615A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102583925A (en) * | 2012-02-03 | 2012-07-18 | 哈尔滨工业大学 | Method for pretreating excess sludge by adopting bio-augmentation technology |
| CN104805032A (en) * | 2014-12-29 | 2015-07-29 | 天津北洋百川生物技术有限公司 | Multifunctional microbial agent for sludge reduction and preparation thereof |
| CN107162175A (en) * | 2017-05-12 | 2017-09-15 | 南京大学 | A kind of method that training degraded penicillin activated sludge is tamed and dociled by co-substrate of glucose |
| CN107162175B (en) * | 2017-05-12 | 2020-06-02 | 南京大学 | Method for domesticating and degrading penicillin activated sludge by taking glucose as co-matrix |
| CN107312725A (en) * | 2017-05-26 | 2017-11-03 | 复环生物科技(上海)有限公司 | One group of functional microorganism flora for promoting to form high-quality ecological fertilizer |
| CN107032574A (en) * | 2017-06-09 | 2017-08-11 | 杜文洁 | Municipal sludge processing composite bacteria agent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gong et al. | Enhancement of anaerobic digestion effluent treatment by microalgae immobilization: Characterized by fluorescence excitation-emission matrix coupled with parallel factor analysis in the photobioreactor | |
| Marchello et al. | Microalgae population dynamics in photobioreactors with secondary sewage effluent as culture medium | |
| CN101033450A (en) | Method of producing composite and highly effective microorganism preparation for waste water treatment | |
| CN106480103B (en) | A kind of detoxification method of ferment antibiotics bacteria residue | |
| CN105174476A (en) | Activated sludge and microalgae coupled granular system for waste water treatment and establishment and operation method thereof | |
| CN110564640B (en) | Siamese bacillus WF2019 strain for degrading aflatoxin B1 and application thereof | |
| KR20040034148A (en) | organic wastes treatment apparatus and method to recycle as a liquid fertilizer | |
| CN103881947B (en) | One strain forms biomembranous defect shortwave Zymomonas mobilis and the application in cyanide wastewater process thereof | |
| CN102234615A (en) | High-efficiency combined functional bacteria for sludge treatment | |
| CN102807954A (en) | Sewage treatment function bacterial | |
| CN107858305A (en) | One plant of tetracycline efficient degradation bacterium and its application | |
| CN103896469A (en) | Preparation method of organic sludge degradation and reduction agent | |
| CN103255060A (en) | A set of organic wastewater degradation composite bacterial and continuous amplification and applications of effective bacterial | |
| CN102703420A (en) | Microbial degradation preparation for municipal garbage and sewage and preparation method thereof | |
| CN102268372A (en) | Efficiently combined functional bacterium for sewage treatment | |
| CN101029298A (en) | Production of efficient microbe bacteria combing agent | |
| CN107365719A (en) | The aerobic flora of one group of quick composting stalk | |
| CN109609407B (en) | Thermophilic microorganism strain for in-situ sludge reduction and application thereof | |
| CN102070283B (en) | Technique for efficiently treating livestock/poultry culture wastewater | |
| CN111621438A (en) | Wedner mannich bacillus LM-LZ separated from oxidation pond of pig farm and application thereof | |
| CN102212475A (en) | High-efficiency combined functional bacteria for sewage treatment | |
| CN101993180A (en) | Method for dung ecological treatment and resource recycle | |
| CN101811893A (en) | Treatment process for improving composting efficiency of domestic garbage | |
| CN111621437B (en) | Otter escherichia coli LM-DK separated from oxidation pond of pig farm and application thereof | |
| CN112574918B (en) | Ammonia nitrogen degrading bacteria, microbial agent and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111109 |