CN102233071B - Traditional Chinese medicine composition for treating depression and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating depression and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating depression. The traditional Chinese medicine composition is prepared by the following raw materials by mass: 1-6 parts of ginseng, 1-5 parts of polygala, 1-5 parts of grass leaved sweetflag, 1-6 parts of poria cocos, 1-6 parts of morinda officinalis, 1-5 parts of radix bupleuri, 1-5 parts of citrus aurantium, 1-6 parts of red paeonia and 1-5 parts of liquorice. The traditional Chinese medicine composition of the invention has the efficacy of invigorating qi, nourishing heart, soothing nerves, regulating qi and coursing liver, promoting blood circulation and relieving depression; and the combination of the medicine has the efficacy of invigorating qi and relieving depression. It is proved by pharmacodynamic tests that the traditional Chinese medicine composition can significantly improve the behavior abnormality of depression model rats with various types, has the efficacy of treating both the symptoms and root causes, and can effectively treat depression.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition, be specifically related to a kind of Chinese medicine composition that is used for the treatment of depression.
Background technology
Depression is the main Types of affective disorders, is a kind of take the low syndrome as principal character of remarkable and lasting mental state.Depression is the able-bodied commonly encountered diseases of harm humans, frequently-occurring disease, it is global at present main spirits problem, its main clinical manifestation is that mood is low, and cause thus the minimizing of other activities in the life, passive such as social activity, enterprising spirit goes down, life is evacuated etc., depression has a strong impact on people's quality of life, and work efficiency is descended, the various invisible loss that cause thus are costly, and some extreme severe patient also may have passive suicide movement.
The Therapeutic Method of modern depression mainly contains psychotherapy, Drug therapy and galvanic shock (tic) treatment.Wherein psychotherapy's method only is suitable for slight case, and middle severe case needs Drug therapy, as for serious case, just uses the method for electric shock treatment.Use at present maximum or Drug therapy, the depressant drug of clinical the most normal use mostly is Western medicine, how just to begin onset in medication two weeks effect, has larger side effect, needs long-term taking, and rebounds easily after the drug withdrawal.And Chinese medicine has advantageous advantage through long-term Clinical Laboratory at aspects such as therapeutic effect, toxic and side effects.Therefore, seek a kind of determined curative effect, side effect is little and the novel anti-depression Chinese medicament of difficult recurrence becomes the focus of antidepressant drug research.
Summary of the invention
The purpose of this invention is to provide Chinese medicine composition of a kind of Cure of depression and preparation method thereof.
The Chinese medicine composition of Cure of depression provided by the present invention is made by the raw material of following mass parts: Radix Ginseng 1-6 part, Radix Polygalae 1-5 part, Rhizoma Acori Graminei 1-5 part, Poria 1-6 part, Radix Morindae Officinalis 1-6 part, Radix Bupleuri 1-5 part, Fructus Aurantii Immaturus 1-5 part, Radix Paeoniae Rubra 1-6 part and Radix Glycyrrhizae 1-5 part.
Described Chinese medicine composition can further be made by the raw material of following mass parts: Radix Ginseng 2-4 part, Radix Polygalae 1-3 part, Rhizoma Acori Graminei 1-3 part, Poria 2-4 part, Radix Morindae Officinalis 2-4 part, Radix Bupleuri 1-3 part, Fructus Aurantii Immaturus 1-3 part, Radix Paeoniae Rubra 2-4 part and Radix Glycyrrhizae 1-3 part.
Described Chinese medicine composition is most preferably made by the raw material of following mass parts: 2 parts in 3 parts of Radix Ginsengs, 2 parts of Radix Polygalaes, 2 parts of Rhizoma Acori Graminei, 3 parts in Poria, 3 parts of Radix Morindae Officinaliss, 2 parts of Radix Bupleuri, 2 parts of Fructus Aurantii Immaturuss, 3 parts of Radix Paeoniae Rubra and Radix Glycyrrhizae.
The source of used each medical material and effect are as follows among the present invention:
Radix Ginseng: be the dry root and rhizome of Araliaceae Radix Ginseng Panax ginseng C.A.Mey..The property sweet, little hardship, flat.Return spleen, lung, heart channel.Have strongly invigorating primordial QI, multiple arteries and veins takes off admittedly, and invigorating the spleen to benefit the lung promotes the production of body fluid, the effect of calming the nerves.
Radix Polygalae: be the dry root of milk wort Radix Polygalae Polygala tenuifolia Willd. or ovum leaf Radix Polygalae Polygala sibirica L..The property bitter, hot, tepor.GUIXIN, kidney, lung meridian.Have the Fructus Alpiniae Oxyphyllae of calming the nerves, eliminate the phlegm, the effect of detumescence.
Rhizoma Acori Graminei: be the dry rhizome of acorus gramineus araceae plant Acorus tatarinowii Schott..The property hot, bitter, temperature.GUIXIN, stomach warp.Has the refreshment of having one's ideas straightened out, removing dampness to restore normal function of the stomach, the effect of the beneficial will of allaying excitement.
Poria: be the dry sclerotia of On Polyporaceae Poria Poria cocos (Schw.) Wolf.The property sweet, light, flat.GUIXIN, spleen, kidney channel.Has inducing diuresis to remove edema, eliminating dampness by diuresis, spleen invigorating, the effect of mind calming.
Radix Morindae Officinalis: be the root of Maguireothamnus speciosus Radix Morindae Officinalis Morinda officinalis How..The property hot, sweet, tepor.Return kidney, Liver Channel.Has reinforcing the kidney and supporting YANG, the effect of expelling wind and removing dampness.
Radix Bupleuri: be the dry root of umbelliferae bupleurum Bupleurum chinensis DC. or Radix Bupeuri Scorzonerfolii. Bupleurumscorzonerifolium Willd..The property bitter, hot, be slightly cold.Return liver, gallbladder meridian.Having induces sweat brings down a fever, dispersing the stagnated live-QI to relieve the stagnation of QI, the effect of elevate a turnable ladder yang-energy.
Fructus Aurantii Immaturus: be the dry young fruit of rutaceae Citrus aurantium Linn. Citrus aurantium L. and variety or Fructus Citri sinensis C.sinensisOsbeck.The property bitter, hot, sour, temperature.Return spleen, stomach, large intestine channel.Has dispelling the stagnated QI except painful abdominal mass, the effect of transforming phlegm and dispersing accumulations.
Radix Paeoniae Rubra: be the dry root of ranunculaceae plant Radix Paeoniae Rubra Paeonia lactiflora Pall. or river Radix Paeoniae Rubra P.veitchii Lynch.The property bitter, be slightly cold.Return Liver Channel.Has clearing away heat and cooling blood, the effect of eliminating stasis to stop pain.
Radix Glycyrrhizae: be the root and rhizome of glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. G.inflata Bat. or Glycyrrhiza glabra L. G.glabra L..
Chinese medicine composition provided by the present invention can be according to following 1)-4) in arbitrary described method be prepared:
1) mixes after will making the raw material pulverizing of described Chinese medicine composition, add water soaking 0.5-2 hour of 6-10 times of quality, decoct 2-3 time again, the each decoction 40-60 minute, collecting decoction filters, filtrate decompression is concentrated into every milliliter of medicinal liquid and is equivalent to crude drug 0.2-3g, namely gets described Chinese medicine composition.
2) will make the Radix Ginseng of described Chinese medicine composition, after broken with the 60-80% ethanol water reflux, extract, of 6-10 times of quality 2-3 time, carry 1-2 hour at every turn, after each extraction is complete, filter, collect merging filtrate, Recycled ethanol is concentrated into the panaxynol extracted extract that every milliliter of medicinal liquid contains the 0.1-1g crude drug with filtrate decompression; Remaining medicine residues of Radix Ginseng merges with other raw material of respectively distinguishing the flavor of of making described Chinese medicine composition after will extracting again, decocting with 6-10 times of quality boils 2-3 time, the each decoction 40-60 minute, collecting decoction, filter, filtrate and described panaxynol extracted extract merge, and further are condensed into every milliliter of medicinal liquid again and are equivalent to crude drug 0.2-3g, namely get described Chinese medicine composition.
That 3) will make described Chinese medicine composition respectively distinguishes the flavor of crude drug after 45-55 ℃ of drying, is broken into first coarse powder, is crushed to fine powder again, crosses at last sieve 6-9 number, namely gets described Chinese medicine composition.
The Radix Ginseng powder that 4) will make described Chinese medicine composition is broken into fine powder, and is stand-by; Other crude drug of respectively distinguishing the flavor of of making described Chinese medicine composition is merged, decocting with 6-10 times of quality boils 2-3 time, the each decoction 40-60 minute, collecting decoction, filter, filtrate decompression is concentrated into every milliliter of medicinal liquid and is equivalent to crude drug 0.2-3g, then adds Radix Ginseng fine powder mixing in concentrated solution, namely gets described Chinese medicine composition.
The medicine of the Cure of depression of making take Chinese medicine composition provided by the present invention as active component simultaneously, also belongs to protection scope of the present invention.
Described medicine can import by the method for oral, external, injection, infiltration, absorption, physics or chemistry mediation body such as muscle, Intradermal, subcutaneous, vein or mucosal tissue; Or mixed by other material or wrap up after import body.
When needing, in said medicine, can also add one or more pharmaceutically acceptable carriers.Described carrier comprises diluent, excipient, filler, binding agent, wetting agent, disintegrating agent, absorption enhancer, surfactant, absorption carrier and the lubricant etc. of pharmaceutical field routine.
The medicine of this Cure of depression can be made tablet, dispersible tablet, hard capsule, soft capsule, granule, pill, micropill, powder, drop pill, decoction, syrup, mixture, oral liquid, soft extract and extractum etc.The medicine of above-mentioned various dosage forms all can be according to the conventional method preparation of Chinese medicine preparation.
The factors such as the dosage form of the usage of above-mentioned Cure of depression medicine and consumption and medicine and patient's age, body weight are relevant.Take decoction as example, adult's consumption is 40-90g (crude drug)/sky.
In the prescription of the present invention, Radix Ginseng strongly invigorating primordial QI, the Fructus Alpiniae Oxyphyllae of calming the nerves, Radix Polygalae calm the nerves Fructus Alpiniae Oxyphyllae, eliminate the phlegm and have one's ideas straightened out, Rhizoma Acori Graminei have one's ideas straightened out refreshment, the mind calming and mentality promoting, Poria spleen invigorating mind calming, Qi-benefiting and heart-nourishing is calmed the nerves, the justice of sedate to get Kaixin San (" formulary of peaceful benevolent dispensary "); Add Radix Morindae Officinalis benefiting qi and supporting yang, eliminating phlegm and relaxing heart, the Radix Bupleuri soothing liver-QI for relieving depression, the Fructus Aurantii Immaturus eliminating stagnation freely stagnates, the Radix Paeoniae Rubra Huoxue Jieyu, the Radix Glycyrrhizae spleen invigorating and in, coordinating the actions of various ingredients in a prescription, get Sini San (" Treatise on Febrile Diseases is distinguished SHAO YIN syndrome arteries and veins card and controls ") soothing the liver, the Huoxue Jieyu of regulating the flow of vital energy; All medicines are played the merit of QI invigorating dissipating depression of QI altogether.Pharmacodynamics test proves that the behavioristics that Chinese medicine composition of the present invention can obviously improve dissimilar depression model rats is unusual, and has the double effect of controlling of standard, effectively Cure of depression.
Below by specific embodiment Chinese medicine composition of the present invention is described, but the present invention is not limited thereto.
Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
Used Radix Ginseng, Radix Polygalae, Rhizoma Acori Graminei, Poria, Radix Morindae Officinalis, Radix Bupleuri, Fructus Aurantii Immaturus, Radix Paeoniae Rubra and Radix Glycyrrhizae all meets the pertinent regulations under each medical material item of text of Chinese Pharmacopoeia version in 2005 among the following embodiment.Before feeding intake, be tested and appraised, ingredients is in kind to conform to title, and quality meets standards of pharmacopoeia.
Embodiment 1, preparation antidepressant composition decoction one
Get 3 mass parts Radix Ginsengs, 2 mass parts Radix Polygalaes, 2 mass parts Rhizoma Acori Graminei, 3 mass parts Poria, 3 mass parts Radix Morindae Officinaliss, 2 mass parts Radix Bupleuri, 2 mass parts Fructus Aurantii Immaturuss, 3 mass parts Radix Paeoniae Rubra, the merging of 2 mass parts Radix Glycyrrhizaes, the water soaking 1 hour that adds 10 times of quality, decoct 1 time, medicinal residues decoct once with 8 times of water gagings again, medicinal residues after decocting for the second time decoct 1 time with 6 times of water gagings again, 1 hour/time, merge three times decocting liquid, filter, filtrate decompression is concentrated into every milliliter of medicinal liquid and is equivalent to crude drug 3g, namely gets happy resolving depression rushing agent one.
Embodiment 2, preparation antidepressant composition decoction two
Get 3 mass parts Radix Ginsengs, after broken with 70% ethanol water reflux, extract, of 10 times of quality 3 times, the time of extracting for 3 times was respectively 1 hour, 40 minutes, 40 minutes, after each extraction is complete, filter, collect filtrate, merge three times filtrate, Recycled ethanol is concentrated into the alcohol-extracted extract that every milliliter of medicinal liquid contains the 0.5g crude drug with filtrate decompression; Remaining medicine residues of Radix Ginseng and 2 mass parts Radix Polygalaes, 2 mass parts Rhizoma Acori Graminei, 3 mass parts Poria, 3 mass parts Radix Morindae Officinaliss, 2 mass parts Radix Bupleuri, 2 mass parts Fructus Aurantii Immaturuss, 3 mass parts Radix Paeoniae Rubra, 2 mass parts Radix Glycyrrhizaes merge after will extracting again, decocting with 10 times of quality boils 3 times, 1h/ time, collecting decoction, filter, when filtrate decompression is concentrated into half volume, concentrated solution and panaxynol extracted extract are merged, reconcentration becomes every mL to contain the 3g crude drug to get final product, namely get happy resolving depression soup and soak decoction two.
Embodiment 3, preparation antidepressant composition powder
Get 3 mass parts Radix Ginsengs, 2 mass parts Radix Polygalaes, 2 mass parts Rhizoma Acori Graminei, 3 mass parts Poria, 3 mass parts Radix Morindae Officinaliss, 2 mass parts Radix Bupleuri, 2 mass parts Fructus Aurantii Immaturuss, 3 mass parts Radix Paeoniae Rubra, 2 mass parts Radix Glycyrrhizaes, put in the baking oven in 50 ℃ of dry 6h, each medicine of distinguishing the flavor of is broken into first coarse powder, be crushed to again fine powder, cross at last No. 6 sieves (flour extraction is greater than 80%), namely get antidepressant composition powder.
Embodiment 4, preparation antidepressant composition mixture
Get 3 mass parts Radix Ginsengs, after broken with 70% ethanol water reflux, extract, of 10 times of quality 3 times, the time of extracting for 3 times was respectively 1 hour, 40 minutes, 40 minutes, after each extraction is complete, filter, collect filtrate, merge three times filtrate, Recycled ethanol is concentrated into about 0.5g crude drug/ml with filtrate decompression; Remaining medicine residues of Radix Ginseng and 2 mass parts Radix Polygalaes, 2 mass parts Rhizoma Acori Graminei, 3 mass parts Poria, 3 mass parts Radix Morindae Officinaliss, 2 mass parts Radix Bupleuri, 2 mass parts Fructus Aurantii Immaturuss, 3 mass parts Radix Paeoniae Rubra, 2 mass parts Radix Glycyrrhizaes merge after will extracting again, decocting with 10 times of quality boils 1 time, decoct once with 8 times of water gagings again, 1h/ time, merge decocting liquid twice, filter, filtrate and panaxynol extract merge, be evaporated to 1g/ml (pressing the crude drug amount calculates), filter fill, every bottle of 60ml, sterilization namely gets the antidepressant composition mixture.
Embodiment 5, preparation antidepressant composition granule
Get 3 mass parts Radix Ginseng powder and be broken into fine powder, cross sieve No. 6; Get 2 mass parts Radix Polygalaes, 2 mass parts Rhizoma Acori Graminei, 3 mass parts Poria, 3 mass parts Radix Morindae Officinaliss, 2 mass parts Radix Bupleuri, 2 mass parts Fructus Aurantii Immaturuss, 3 mass parts Radix Paeoniae Rubra, the merging of 2 mass parts Radix Glycyrrhizaes, decocting with 10 times of quality boils 2 times, 1h/ time, collecting decoction, filter, filtrate decompression is concentrated into 1g/ml (press crude drug amount calculate), then adds extrusion granulator behind ginseng crude drug's fine powder mixing in the concentrated solution, is drying to obtain the antidepressant composition granule.
Pharmacodynamic experiment
Embodiment 6, happy resolving depression soup merge the pharmacodynamics test of chronic stress depression of sex rat model behavior and body weight impact on high fat
(1) test material
1, medicine:
Negative control product: distilled water
Positive reference substance: fluoxetine Hydrochloride
The happy resolving depression rushing agent of test drug: embodiment 1 preparation
2, experimental animal: SD rat
(2) high fat merges the foundation of chronic stress depression of sex rat model
The reference literature method (Pan Juhua, Wang Yanyun, Huang Shijing, etc. the foundation of Rat Model of Vascular Depression.Capital University of Medical Sciences's journal, 2007,28 (6): 761-762) make the vascular Depression model.Front 4 groups are organized intramuscular injection VD for rear four to normal diet
37.5mg/kg body weight for three days on end, then gives high lipid food (high lipid food prescription: yolk powder 10%, Adeps Sus domestica 10%, cholesterol 1%, sodium cholate 0.2%, normal diet 79%), use simultaneously propylthiouracil (0.15g/kg body weight) gavage, 2 times weekly.All animals claim weekly 1 body weight and edible forage volume, extract 3 normal diet rats after 9 weeks and 3 high lipid food rats detect blood fat and do aortal pathological examination, proves hyperlipidemia formation, but have no obvious arteriosclerosis.Each treated animal is carried out after open field test and sucrose solution consumption test detect, and except Normal group continued normal the raising, all the other each groups adopted chronic Unpredictability gentleness (CUMS) to carry out 3 all depression modelings, formed high fat stack CUMS depression model.CUMS reference literature (Willner P, TowellA, Sampson D, et al1 Reduction of sucrose preference by chronic mild unpredictable stressand its restoration by a tricyclic antidepressantl Psychopharmacology, 1987,93:3582-3641.) method is carried out, animal is pressed from both sides tail 1min, prohibit water 24h, fasting 24h, 45 ℃ of environment 5min, light and shade is put upside down 24h, electric shock vola 10s (80v, 10 times, each random interval 10-50s), empty bottle is placed 1h, moist bedding and padding 24h, mouse cage inclination 24h, 4 ℃ of methods such as frozen water swimming 5min stimulate.These stimulate takes a kind ofly every day at random, in totally 3 weeks, makes animal can not expect to stimulate and occurs.
(3) test method
1. materials and methods
1.1 medicine and reagent
Happy resolving depression soup: provide (according to the preparation of embodiment 1 method), lot number: 080605 by the Gate of Pervasive Peace hospital chamber of grinding.Fluoxetine Hydrochloride, gift come Suzhou pharmaceutical Co. Ltd, lot number: 070608.Sucrose: Chemical Reagent Co., Ltd., Sinopharm Group, lot number: 20080111.Vitamin D3 injection: Shanghai General Pharmaceutical Co., ltd., lot number: 061002.
1.2 grouping
Rat conformed after a week, carried out sucrose solution consumption test and open field test, according to two test scores, carry out layering after, again by the body weight grouping.Be divided into and be 8 groups such as normal control, depression, depression+Chinese medicine, depression+Western medicine, high fat, vascular Depression, vascular Depression+Chinese medicine, vascular Depression+Western medicine, every group of 12 animals.
1.3 administration and detection
In depressed modeling, begin administration.Chinese drug-treated group gives happy resolving depression soup 22g crude drug/kg, and the Western medicine group gives fluoxetine Hydrochloride 2.4mg/kg, and all the other each groups are given with the volume distilled water, and once a day, gastric infusion is 22 days continuously.Modeling 1, weigh in 8,15,22 days the time and measure sucrose solution consumption and open field test, abdominal aortic blood is surveyed the biochemical indicators such as blood fat after 22 days.Carry out statistical procedures with SPSS software.
2 observation index
2.1 body weight is front respectively at experiment, depressed 1,8,15,22 day record rat body weight.
2.2 sucrose solution consumption experiment
Reference literature method (D ' Aquila PS, Brain P, Willner P.Effects of chronic mild stress onperformance in behavioural tests relevant to anxitety and depression.PHYSIOL Behav, 1994,56 (5): 861-867.), carry out altogether 5 times, before experiment, carry out in the time of depressed 1,8,15,22 day.Single cage is raised during experiment, and sucrose solution is drunk in the animal training adaptation in quiet room, and every cage was placed 2 bottles of sucrose solution (1% sucrose solution) in the 1st day; The 2nd day 1 bottle of sucrose solution, 1 bottle of plain boiled water, 2 bottles of positions are at random; Water is prohibited in fasting in the 3rd day.Place at random 2 water bottles in the 4th day every rat, be placed with respectively 1% sucrose water and the plain boiled water of weighing in advance, behind the 120min, get 2 bottles of water and again weigh.Record sucrose solution consumption, the plain boiled water consumption of every rat, calculate sucrose solution and consume percentage rate.
2.3 open field test
Reference literature method (Katz R J, Roth K A, Carroll RJ.Acute and chronic stress effects on openfield activity in the rat:implicarjons for a model of depression.Neurosci Biobehav Rev1981,5 (2): 247-51.), carry out altogether 5 times, before experiment, carry out in the time of depressed 1,8,15,22 day.The spacious case of self-control, high 42cm, the bottom surface is 76 * 76cm
2Square.Around the tank wall and bottom surface is painted with black, and the bottom surface 25 large grids such as is divided into white line.The rat four paws enters counts walking 1 lattice in the same grid, two fore paws are liftoff to be counted and stand 1 time.In the quiet room, open field test is arranged in 8:30-11:30 in the morning, and every rat is tested separately, keeps clean in the spacious case before each test.Count every number of times that rat walks and stands in the 5min with enumerator, count 1 minute at every turn.Add up respectively the goals for of standing and walk, carry out statistical procedures.
2.4 lipids detection
Intraperitoneal anesthesia, abdominal aortic blood carries out lipids detection with AU640 type full-automatic biochemical detector.
2.5 statistical procedures: carry out one factor analysis of variance with SPSS11.5.
(4) result of the test
1, respectively organizes rat fat situation (seeing table 1 for details)
The rat fat situation is respectively organized in the depressed modeling of table 1 in the time of 22 days
Compare * P＜0.05, * * P＜0.01, * * * P＜0.001 with Normal group
Compare #P＜0.05, ##P＜0.01 with high fat matched group
2 impacts on rat sucrose solution consumption (seeing table 2 for details)
Table 2 is respectively organized rat different times sucrose solution and is consumed percentage comparisons
Compare * P＜0.05, * * P＜0.01 with the depression group.
Compare #P＜0.05 with the depressed group of blood vessel.
3 impacts on the rat behavior activity (seeing table 3 for details)
Table 3 is respectively organized the rat different times and is walked lattice number of times relatively (n=12)
Compare * P＜0.05, * * P＜0.01, * * * P＜0.001 with the depression group
Compare #P＜0.05, ##P＜0.01, ###P＜0.001 with strongly fragrant group of pressing down property of blood vessel
This experimental result shows: CHO, LDL content obviously raise in the animal blood of edible high lipid food, the proof hyperlipidemia forms, compare with high fat matched group, Chinese medicine intervention group CHO obviously descends (P＜0.05), illustrates that happy resolving depression soup has certain effect for reducing blood fat; Experiment also shows: the hyperlipidemia animal does not have obvious depressive symptom, and simple depression model and vascular Depression model all have obvious Depression, and two groups do not show significant difference in sucrose solution consumption and spacious experiment; Happy resolving depression soup and fluoxetine Hydrochloride all can increase animal to the preference of sucrose solution, strengthen animal horizontal anomalous movement degree and space exploration ability, show that medicine all has obvious inhibitory action to two kinds of depression models, but in the vascular Depression model, the curative effect of happy resolving depression soup slightly is better than fluoxetine Hydrochloride.
Result of study shows in this experiment: compare the growth of model group rat body weight with normal group and obviously slow down, the medication group obviously improves than the model group body weight gain by rat, in administration in the time of 22 days, depressed Chinese drug-treated group, depressed Western medicine group body weight are apparently higher than depression model matched group (P＜0.05), the vascular Depression Chinese drug-treated group is apparently higher than vascular Depression model group (P＜0.05), although vascular Depression Western medicine group body weight also has rising, there are no statistical significance.Show happy resolving depression soup to the anorexia of patients with depression, weight loss can be improved effect.
Embodiment 7, happy resolving depression soup merge the pharmacodynamics test of chronic stress depression of sex rat model antioxidation to high fat
(1) test material
1, medicine:
Negative control product: distilled water
Test drug: happy resolving depression rushing agent one
2, experimental animal: SD rat
(2) high fat merges the foundation of chronic stress depression of sex rat model, with embodiment 6.
(3) test method
1, animal grouping
After Animal adaptability raised for 1 week, adopt the Open-field method to carry out Behavioral assessment, select 72 close of rats of scoring to test 12 every group.(1) Normal group: give normal diet, normal drinking-water; (2) depressed group: give normal diet, normal drinking-water.Chronic unpredictable stress (CUMS).(3) high fat group: give high lipid food, VD3 load, normal drinking-water; (4) vascular Depression group: give high lipid food, VD3 load, in addition CUMS method; (5) depressed treatment group: add the intervention of happy resolving depression soup on the basis of depression group.(6) vascular Depression treatment group: add the intervention of happy resolving depression soup on the basis of vascular Depression group.Each group of administration is not given the equivalent distilled water.
2, happy resolving depression soup test sample preparation
Take by weighing 100 doses by recipe quantity, be total to 6.6Kg, add 10 times of water gagings and soaked 1 hour, decoct 1 time, medicinal residues add 8 times of water gagings again and decoct once, medicinal residues after decocting for the second time decoct 1 time with 6 times of water gagings again, 1 hour/time, merge three times decoction liquor, filter, filtrate is concentrated into every milliliter and is equivalent to crude drug 3g, altogether 2200ml.(pressing the method preparation of embodiment 1)
3, detect NO, ET, MDA, SOD
NO, ET, MDA, SOD all adopt Nanjing to build up the test kit that bio-engineering research is produced.NO detects and adopts chemical method.ET by PLA General Hospital Science and Technology Development Center put exempt from adopt to put and exempt from method and detect.
4, statistical procedures
Summarized materials, all the data input computer adopts SPSS 11.5 for windows, adopts variance analysis between many groups, adopts the T check to carry out data analysis between two groups.
(4) result of the test
During grouping, each organizes the equal bouncing of rat, hair luster, and body weight is without significant difference between two groups.After the modeling, vascular Depression group compared with normal matched group loses weight, the movable minimizing, and hair is withered, tarnish.
Serum NO level, ET, MDA, SOD detect: compare with Normal group, hyperlipidemia model group, depressed matched group and vascular Depression matched group serum MDA all significantly raise (p＜0.05), and each is organized SOD and obviously descends, and NO and ET change not obvious.After using happy resolving depression soup intervention, SOD obviously raises, and with vascular Depression model group ratio notable difference is arranged.The results are shown in Table 4.
Table 4 respectively organize rat NO, ET, MDA, SOD testing result relatively (n=12,
± s)
Annotate: compare with Normal group: (2) p＜0.05, p＜0.01, (3) p＜0.001;
Compare with the vascular Depression group: (a) p＜0.05, (b) p＜0.01, (c) p＜0.001.
Originally studies show that the happy resolving depression soup SOD that can raise in the chronic stress rat plasma is active, and reduce MDA content in the chronic stress rat plasma, point out the antidepressant effect of happy resolving depression soup may be with its enhancing activities of antioxidant enzymes, to remove free radical, anti peroxidation of lipid too much in the body relevant.
Embodiment 8, happy resolving depression soup merge the pharmacodynamics test of monoamine neurotransmitters impact in the chronic stress depression of sex rat model brain on high fat
(1) test material
1, medicine:
Negative control product: distilled water
Positive reference substance: fluoxetine Hydrochloride
Test drug: happy resolving depression soup two
2, experimental animal: SD rat
(2) high fat merges the foundation of chronic stress depression of sex rat model, with embodiment 6.
(3) test method
1 medicine and reagent
(gift comes Suzhou pharmaceutical Co. Ltd to fluoxetine Hydrochloride, lot number: 070608); Happy resolving depression soup: provide lot number by the Gate of Pervasive Peace hospital chamber of grinding: 080605.NE, 5-HT, DA, HVA, 3,4 dihydroxy benzylamines (DHBA),
1-perfluorooctane sulfonate, monohydrate potassium, trisodium citrate, disodiumedetate are the Sigma product; Methanol is Merck company product; Sucrose, perchloric acid and other reagent are domestic AR level; Experimental water is ultra-pure water.
2 preparations for the reagent product
Happy resolving depression soup: according to the method preparation of embodiment 2, lot number: 080605.Fluoxetine Hydrochloride: the mortar porphyrize adds normal saline and is made into the solution that concentration is 0.24mg/mL.
The grouping of 3 animals
Male SD rat carries out sucrose solution consumption test and open field test after the suitable week, is divided into normal control, depression model, depression+Chinese medicine and depression+Western medicine group according to two test scores and body weight, and every group of 12 animals, all animals claim weekly 1 body weight and forage volume.
The mensuration of 5-HT, NE, DA and HVA in the 4 brain cortex
The rat aorta sacrificed by exsanguination, cut off scalp, separate cerebral dura mater and skull, peel off cerebral tissue at ice pan, remove the blood of adhesion with 4 ℃ of cold saline flushings, use the filter paper suck dry moisture, clip brain cortex, choose 6 complete specimen of separation for every group and put liquid nitrogen flash freezer in the cryopreservation tube ,-80 ℃ of cryopreservation are to be measured.The about 20-30mg brain of clip cortex sample places 1.5mL EP pipe, and accurately weighing adds the ice-cold sample pretreatment A of 200 μ L liquid (0.4molL
-1Perchloric acid), the interior mark of the DHBA liquid 2 μ L that add 5ng μ L, (0.5HZ namely vibrates 1s to ultrasonic homogenate 10s in the ice bath, intermittently 1s, repeat 4～5 times), lucifuge leaves standstill 1h after the homogenate, 4 ℃ of centrifugal (12000rpm) 20min, draw supernatant 160 μ L, the ice-cold sample pretreatment B liquid that adds 80 μ L (contains 20mmolL
-1Potassium citrate, 300mmolL
-1Dipotassium hydrogen phosphate, 2mmolL
-1The aqueous solution of EDTA2Na), the vortex mixing, ice bath leaves standstill 1h, and 4 ℃ of centrifugal (12000rpm) 20min draw supernatant 200 μ L, and filtering with microporous membrane is measured by following chromatographic condition at once, and test sample product-80 ℃ do not keep in Dark Place.Chromatographic condition: Zorbax Eclipse XDB-C
18(4.6mm * 150mm, 5 μ m) chromatographic column, EasyGuard guard column (Dikma company); To contain monohydrate potassium 80mmolL
-1, trisodium citrate 73.4mmolL
-1, 1-perfluorooctane sulfonate 0.12mmolL
-1, disodiumedetate 0.1mmolL
-1, the mixed solution that contains 18% methanol is mobile phase (pH4.3); Flow velocity: 0.5mLmin
-1Column temperature: 25 ℃; Injector temperature: 4 ℃; Sample size: 10 μ L; Measure electrode potential :-150mv and 400mv.
(4) result of the test
The result of variations of 5-HT, NE, DA and HVA sees Table 5 in the brain cortex.Compare with normal group, the content of 5-HT, NE, DA and HVA all reduces in the model group rat brain cortex, and wherein 5-HT and NE change significant difference (P＜0.01 or 0.05), and DA and HVA content are obvious, but there was no significant difference.Compare with model group, 5-HT, NE, DA and HVA content are improved in the rat brain cortex of happy resolving depression soup group and fluoxetine Hydrochloride group, wherein 5-HT and NE have significance to raise (P＜0.01 or 0.05), and DA and the HVA content of part group have significance rising (P＜0.05).
Table 5 is respectively organized the variation (mean ± SD, n=6) of 5-HT and NE in the rat brain cortex
Compare * P＜0.05, * * P＜0.01 with the depression group.
The equal compared with normal group decline of the content of 5-HT, NE, DA and HVA in the brain cortex of rat model in this experiment, wherein 5-HT and NE change significant difference (P＜0.01 or 0.05).Fluoxetine is selectivity 5-HT reuptake inhibitor, and the alternative reuptake that suppresses 5-HT improves 5-HT level in the brain, thus Cure of depression.After happy resolving depression soup and fluoxetine Hydrochloride treatment, compare with model group, 5-HT, NE, DA and HVA in the rat brain cortex (DA is through the metabolite of monoamine oxidase, MAO approach) content is improved, wherein 5-HT and NE have significance rising (P＜0.01 or 0.05), the DA of part group and HVA content is significantly rising (P＜0.05) also, it is unbalance to point out happy resolving depression soup can correct the Brain Monoamines mediator, can increase 5-HT in the cortex, NE and DA content, thereby correction model mouse behavioristics is unusual.
Embodiment 9, happy resolving depression soup merge the pharmacodynamics test that chronic stress depression of sex rat model cortex ICAM-1 expresses to high fat
(1) test material
1, medicine:
Negative control product: distilled water
Test drug: happy resolving depression soup two
2, experimental animal: SD rat
(2) high fat merges the foundation of chronic stress depression of sex rat model, with embodiment 6.
(3) test method
1, laboratory animal grouping
After Animal adaptability raised for 1 week, adopt the Open-field method to carry out Behavioral assessment, select 18 close of rats of scoring to test 6 every group.(1) blank group: give normal diet, normal drinking-water; (2) vascular Depression group: give high lipid food, VD3 load, in addition Chronic unpredictable stress CUMS method; (3) vascular Depression treatment group (Chinese medicine): add the Chinese medicine intervention on the basis of vascular Depression group.Each group of administration does not give the equivalent distilled water.
2, happy resolving depression soup test sample preparation
Press the method preparation of embodiment 2.
3, method
3.1 tissue slice preparation
Last behavioristics test and to get blood complete, broken end is got brain, and cerebral morphology is observed, and cerebral tissue is carried out paraffin embedding, cuts into slices the thick 4 μ m of sheet.
3.2 immunohistochemical experiment operating procedure
1, dewaxing, aquation tissue slice
Before the dewaxing, tissue slice was toasted 30 minutes in 80 ℃ of calorstats.
1) tissue slice places dimethylbenzene to soak 10 minutes, soaks 10 minutes behind the replacing dimethylbenzene again;
2) soaked 5 minutes in the dehydrated alcohol;
3) soaked 5 minutes in 95% ethanol;
4) soaked 5 minutes in 70% ethanol;
5) fully washing.
2,3%H
2O
2Deionized water is hatched 10 minutes (container is added a cover), with the blocking-up endogenous peroxydase.
3, Microwave method: high-grade 10 minutes of microwave.
4, PBS flushing, 1 time 2 minutes, totally 3 times.
5, dry or blot surrounding liquid, drip primary antibodie, put into wet box, 4 ℃ are spent the night, PBS flushing, 1 time 2 minutes, totally 3 times.
6, drip 1,37 ℃ of reagent and hatched 20 minutes, PBS flushing, 1 time 2 minutes, totally 3 times.
7, drip 2,37 ℃ of reagent and hatched 20 minutes, PBS flushing, 1 time 2 minutes, totally 3 times.
8, use the colour developing of DAB solution.DAB-H
2O
2Colour developing, in the Microscopic observation colour developing situation control time, tap water fully washes color development stopping.
9, redye: after haematoxylin redyed for 20 seconds, washing; The differentiation of 1% acidic alcohol, washing; After 30 seconds, fully wash in the ammonia.
10, mounting: gradient dehydration (70%, 95% ethanol), dehydrated alcohol dehydration, each 5 minutes, 2 times; After drying, the neutral gum mounting treats that the complete drying of its glue is placed on the section box and preserves, with result to be seen.
3.3 microscopic examination, photograph and image processing and analyzing
Adopt professional image analysis software IPP6.0 that graphical analysis is carried out in section, the section amplification represents the object lens magnification eyepiece amplification that multiplies each other.Get the similar Serial tissue sections of each rat section level, all sections are carried out in same intensity, under amplifying 100 times.
3.4 statistical analysis
All data use spss11.5 software to carry out statistical analysis.Each is organized data and all represents with mean ± standard deviation, Group Design multisample mean relatively with the ANOVA check, per two means are relatively checked with q between a plurality of sample averages.
(4) result of the test
ICAM-1 positive reaction thing is positioned at neuron plasma under the light microscopic, be deep mixed brown yellow granule shape, can obviously find out nuclear and the neurite of engrain in it, the result shows the positive neuron number of vascular Depression group rat more than matched group, and difference has significance (P＜0.05).The positive neuron number of vascular Depression treatment group rat is less than the vascular Depression group, and visible less endochylema is the slightly granular positive cell of sundown, and positive cell number obviously reduces, property that there were significant differences (P＜0.05).Concrete outcome sees the following form 6.
Table 6 is respectively organized the positive expression result of rat cerebral tissue
Annotate: * vascular Depression group is compared with blank group and is all risen office, P＜0.05;
▲ vascular Depression treatment group is compared with the vascular Depression group and is all reduced P＜0.05.
This experimental studies results shows, the positive expression of the cerebral tissue of vascular Depression treatment group rat is less than the vascular Depression group, can illustrate that happy resolving depression soup is improved effect to the high expressed of intercellular adhesion molecule-1 (ICAM-1), illustrate that also happy resolving depression soup has therapeutical effect to Rat Model of Vascular Depression simultaneously.
Embodiment 10, happy resolving depression soup merge the pharmacodynamics test of chronic stress depression of sex rat model cerebral tissue 5-HT expression of receptor to high fat
(1) test material
1, medicine:
Negative control product: distilled water
Positive reference substance: fluoxetine Hydrochloride
Test drug: happy resolving depression soup two
2, experimental animal: SD rat
(2) high fat merges the foundation of chronic stress depression of sex rat model, with embodiment 4.
(3) test method
1, grouping
Rat conformed after 1 week, carried out sucrose solution consumption test and open field test, according to two test scores, carry out layering after, again by the body weight grouping.Be divided into and be normal control, depression, depression+Chinese medicine, 4 groups of depression+Western medicine, every group of 8-12 rat.
2, medicine and reagent
Happy resolving depression soup: provide (method according to embodiment 2 is prepared), lot number: 080605 by the Gate of Pervasive Peace hospital chamber of grinding.Fluoxetine Hydrochloride, Suzhou pharmaceutical Co. Ltd, lot number: 070608.Sucrose: Chemical Reagent Co., Ltd., Sinopharm Group, lot number: 20080111.Trizol total RNA extraction reagent: American I nvitrogene company; CDNA test kit: American AB I company; PCR kit for fluorescence quantitative (Super Green Power Mix)
3, administration and detection
In depressed modeling, begin administration.Chinese drug-treated group gives happy resolving depression soup 22g crude drug/kg, and the Western medicine group gives fluoxetine Hydrochloride 2.4mg/kg, and all the other each groups are given with the volume normal saline, once a day, and continuous gastric infusion 22d.
4, detect index
The impact of brain cortex and hippocampus 5-HT 1A/2A Receptor mRNA: it is complete that rat aorta is got blood, immediately cephalotomy scissors beginning skin, separate brain dura mater and skull, peel off cerebral tissue on ice, with the blood that 4 ℃ of pre-cooling normal saline flushings adhere to, filter paper blots, cut respectively cerebral cortex district and hippocampus, each adds 0.5ml pre-cooling Trizol, and after the careful fast grinding homogenate, frozen preparing against in-80 ℃ of refrigerators extracted total RNA.When extracting total RNA sample taken out and is put in 4 ℃ on ice, add again 0.5ml Trizol after, leave standstill 5min under the room temperature; Add 200 μ l chloroforms, behind the violent jolting 15s mixing, room temperature leaves standstill 3min; 4 ℃, the centrifugal 15min of 12000rpm, RNA is distributed in aqueous phase; The colourless water in upper strata is transferred in another Ep pipe, added 500 μ l isopropyl alcohols, room temperature leaves standstill 10min; 4 ℃, the centrifugal 10min of 12000rpm; Abandon supernatant, with 1ml75% washing with alcohol RNA precipitate, 4 ℃, the centrifugal 5min of 7500rpm; Abandon supernatant, drying at room temperature 15min; Add 40 μ lDEPC water dissolution precipitate in the precipitate of crossing to drying, after measuring OD260/280 and determining the concentration and purity of total RNA, use in order to carrying out reverse transcription in-20 ℃ of preservations.Then according to cDNA test kit and real-time fluorescence quantitative PCR test kit step, carry out reverse transcription and pcr amplification.Primer sequence (synthetic by match Parkson, Beijing gene technology company limited) is respectively: 5-HT-1AR: upstream aagaagagcctgaacggaca (sequence 1 in the sequence table), downstream cagaggaaggtgctctttgg (sequence 2 in the sequence table); 5-HT-2AR: upstream agctctgtgcgatctggatt (sequence 3 in the sequence table), downstream cccctccttaaagaccttcg (sequence 4 in the sequence table); Confidential reference items GAPDH: upstream agacagccgcatcttcttgt (sequence 5 in the sequence table), downstream cttgccgtgggtagagtcat (sequence 6 in the sequence table).
(4) result of the test
1, respectively organizes the change of rat hippocampus district 5-HT 1A/2A Receptor mRNA
In the model group Rat hippocampus, 5-HT1AR mRNA level descends, and 5-HT2AR mRNA level raises (comparing p＜0.05 with Normal group), all can reduce to some extent behind Chinese medicine and the western medicine.
2, respectively organize the change of rat cerebral cortex district 5-HT 1A/2A Receptor mRNA
In cerebral cortex, the change that the change of 5-HT1AR and 5-HT2AR mRNA level and the change in the inferior colliculus cerebral tissue present mutual antagonism.
This result of study shows: CUMS can cause that the corresponding behavioristics of rat changes, and induces to form the Depression in Rats model.Happy resolving depression soup can reduce in the hypothalamus 5-HT2A receptor mrna level in the 5-HT1A receptor mrna and cerebral cortex, the 5-HT1A receptor mrna level in the 5-HT2A receptor mrna and cerebral cortex that raises simultaneously in the hypothalamus, and part realizes that it is to the therapeutical effect of depression.Result of study provides certain scientific basis for further disclosing happy resolving depression soup Cure of depression.
Embodiment 11, happy resolving depression soup are to the pharmacodynamics test of chronic cerebral ischemia depression model rat
(1) test material
1, medicine:
Negative control product: distilled water
Test drug: happy resolving depression decoction pulvis
2, experimental animal: SD rat
(2) the permanent ligation of bilateral carotid arteries causes the foundation of chronic cerebral ischemia depression model rat
After Animal adaptability raised for 1 week, be divided at random operation group and Sham-operated control group, the operation group is divided into again model control group and treatment by Chinese herbs group.Adopt the permanent ligating methods of bilateral common carotid arteries to prepare cerebral ischemic model, concrete grammar is as follows: fasting 12h before the rat art.With chloral hydrate (35mg/kg) intraperitoneal injection of anesthesia, guarantee that intra-operative has autonomous respiration.It is fixing to lie on the back, and cervical region unhairing, Iodophors sterilization tailing edge neck medisection are separated bilateral common carotid arteries, and is embedded with " 5-0 " number line.Distinguish the distance end of ligation bilateral common carotid arteries, and cut off from the centre, to guarantee the interruption artery blood flow.Insulation is noted in operation, delivers to the Animal House of heating ventilation and air-conditioning equipment and raise after animal is clear-headed.Sham-operated control group is not cut off the bilateral common carotid arteries except not ligation, and all the other processes are identical with experimental group.
(3) test method
1, the preparation of Chinese medicine test sample and application
1.1 the preparation of experiment test sample
Press the method preparation of embodiment 3.With pulverizing after the commercially available medical material oven dry, make ultramicro medicine powder.Add the method preparation of 0.5%CMC-Na to 100ml by the little medicated powder of 18g (being the 18g crude drug).Dosage is 1.8g crude drug/kg/d.
1.2 the application of experiment test sample
Postoperative next day, treatment by Chinese herbs group rat gives happy resolving depression side 1ml/100g/d gavage, continuous three days; The rat of model control group and Sham-operated control group gives drinking water 1ml/100g.d gavage, for three days on end.
2 behavioristicss are detected
Adopt Morris water maze method to carry out the mensuration of Rats With Memory function.Morris water maze (MorrisWaterMaze, MWM) is produced by Chengdu TME Technology Co., Ltd., is a stainless round pool, diameter 130cm, high 50cm.Pool wall is indicated 4 place of entry, thus the pond is divided into 4 quadrants, optional wherein 1 quadrant, and the platform of 1 diameter 10cm, high 23cm is placed in the center.Laboratory temperature remains on 24 ℃-25 ℃, and the water surface exceeds security platform 1cm, and water temperature is controlled at (26 ± 1) ℃.There is abundant georeferencing clue (door, window, lamp, experimenter etc.) around the pond, and remains unchanged for the rat locating platform.Labyrinth top is equipped with video camera, and is connected the display connection with videocorder, and automatic input rats'swimming track is analyzed.Test program is orientation navigation test (place navigation): last 6d, every morning 2 times, rat is put into water from 2 place of entry respectively towards pool wall, and recording its time that searches out and climb up platform in 90s is escape latency (escape latency).If rat is not found platform at 90s, then draw it by the experimenter to platform with hands, allow rat stop 10s, put back to again in the cage, be designated as best result 90s incubation period.After the operation, re-started water maze laboratory in 3 days after surgery, rat searched out at every turn and climbed up platform in 90s time is the Memory result of rat, in morning 2 times on the same day, calculates average achievement.
3CBF measures
Brain solid positioner (Chengdu TME Technology Co., Ltd.'s production) is fixed in rear the 3rd day of operation behind the rat anesthesia, by " rat brain stereotaxic atlas ", cut off calvarium skin, fully exposes skull.Fibre-optical probe PROBE 418-1 is fixed with support, and frontal lobe CBF measures and selects the front 3mm of bregma, the other 2mm point of center line; 4mm, the other 2mm point of center line behind the top selection bregma.Stick at measuring point with biogum.With the PeriFlux System5000 type laser Doppler flowmetry (LDP) that Sweden Perimed AB company produces, laser diode light source is 2mW chloro-Ne laser, and wavelength is 6328nm.The CBF of every rat frontal lobe of METHOD FOR CONTINUOUS DETERMINATION, top changes 6min, and record LDP output signal is through PERISOFT routine processes, calculating mean value.
4 statistical analysis
This experiment respectively organize the data result all with
± s represents, relatively adopts the t check between experimental group and matched group, relatively adopts in twos one factor analysis of variance to carry out statistical procedures between the experimental group group.
(4) result of the test
1, memory ability is measured after the permanent ligation of different group rats with bilateral common carotid artery
At first occur of short duration convulsions after bilateral common carotid arteries is blocked fully, breathing is slowed down; Postoperative Early observation animal movement reduces, and hind leg is " Eight characters ", the ataxia of walking, or creep and turn-take.The Morris water maze is the result can find out, respectively organizes rat through what training had learned to swim the labyrinth, and the permanent ligation of bilateral common carotid arteries is after 3 days, and memory function descends, and swimming time prolongs.Learn by statistics relatively, model control group perioperatively data comparing difference is (P＜0.05) significantly; Treatment by Chinese herbs group and Sham-operated control group perioperatively data comparing difference are without significance (P＞0.05); The more equal no significant difference of data (P＞0.05) before the operation between three groups; The rear data of operation relatively between three groups: data and the rear data comparing difference for the treatment of by Chinese herbs group operation are without significance (P＞0.05) after the model control group operation, data and the rear data comparing difference of Sham-operated control group operation remarkable (P＜0.05) after the model control group operation, data and the rear data comparing difference of Sham-operated control group operation are without significance (P＞0.05) after the operation for the treatment of by Chinese herbs group; Data see Table 7.
Table 7 rat water maze laboratory achievement
* postoperative and model control group postoperative contrast P＜0.05
The mensuration of CBF after the permanent ligation of 2 different group rats with bilateral common carotid artery
After the permanent ligation of rats with bilateral common carotid artery 3 days, learn by statistics relatively, the CBF data of three group cortex of frontal lobe relatively: model control group and treatment by Chinese herbs group comparing difference are without significance (P＞0.05); Model control group and Sham-operated control group be comparing difference remarkable (P＜0.05) relatively; Treatment by Chinese herbs group and Sham-operated control group be comparing difference remarkable (P＜0.05) relatively.The CBF data of three group cortex of parietal lobe compare: model control group and treatment by Chinese herbs group comparing difference are without significance (P＞0.05); Model control group and Sham-operated control group be comparing difference remarkable (P＜0.05) relatively; Treatment by Chinese herbs group and Sham-operated control group be comparing difference remarkable (P＜0.05) relatively.Data see Table 8.
Frontal lobe, top CBF measurement result after the permanent ligation of table 8 rats with bilateral carotid artery
* Sham-operated control group contrasts P＜0.05 with model control group, treatment by Chinese herbs group respectively
Result of study shows that the permanent ligation of bilateral carotid arteries causes the cerebral ischemia phenomenon of rat fairly obvious, and the behavioristicss such as water maze that happy resolving depression soup can improve the depression model rat change, and improve the cerebral blood flow of depression model rat.
Sequence table
<160>6
<210>1
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>1
aagaagagcc tgaacggaca 20
<210>2
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>2
cagaggaagg tgctctttgg 20
<210>3
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>3
agctctgtgc gatctggatt 20
<210>4
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>4
cccctcctta aagaccttcg 20
<210>5
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>5
agacagccgc atcttcttgt 20
<210>6
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<223>
<400>6
cttgccgtgg gtagagtcat 20
Claims (10)
1. the Chinese medicine composition of a Cure of depression is made by the raw material of following mass parts: Radix Ginseng 1-6 part, Radix Polygalae 1-5 part, Rhizoma Acori Graminei 1-5 part, Poria 1-6 part, Radix Morindae Officinalis 1-6 part, Radix Bupleuri 1-5 part, Fructus Aurantii Immaturus 1-5 part, Radix Paeoniae Rubra 1-6 part and Radix Glycyrrhizae 1-5 part.
2. Chinese medicine composition according to claim 1, it is characterized in that: described Chinese medicine composition is made by the raw material of following mass parts: Radix Ginseng 2-4 part, Radix Polygalae 1-3 part, Rhizoma Acori Graminei 1-3 part, Poria 2-4 part, Radix Morindae Officinalis 2-4 part, Radix Bupleuri 1-3 part, Fructus Aurantii Immaturus 1-3 part, Radix Paeoniae Rubra 2-4 part and Radix Glycyrrhizae 1-3 part.
3. Chinese medicine composition according to claim 2, it is characterized in that: described Chinese medicine composition is made by the raw material of following mass parts: 2 parts in 3 parts of Radix Ginsengs, 2 parts of Radix Polygalaes, 2 parts of Rhizoma Acori Graminei, 3 parts in Poria, 3 parts of Radix Morindae Officinaliss, 2 parts of Radix Bupleuri, 2 parts of Fructus Aurantii Immaturuss, 3 parts of Radix Paeoniae Rubra and Radix Glycyrrhizae.
4. arbitrary described Chinese medicine composition according to claim 1-3, it is characterized in that: the preparation method of described Chinese medicine composition, mix after comprising the steps: to make the raw material pulverizing of described Chinese medicine composition, add water soaking 0.5-2 hour of 6-10 times of quality, decoct 2-3 time, decocted 40-60 minute at every turn, merge decoction liquor, filter, filtrate decompression is concentrated into every milliliter of medicinal liquid and is equivalent to crude drug 0.3-3g, namely gets described Chinese medicine composition.
5. arbitrary described Chinese medicine composition according to claim 1-3, it is characterized in that: the preparation method of described Chinese medicine composition, comprise the steps: to make the Radix Ginseng of described Chinese medicine composition, with the 60-80% ethanol water reflux, extract, of 6-10 times of quality 2-3 time, carry 1-2 hour after broken at every turn, extract at every turn complete after, filter, collect merging filtrate, Recycled ethanol is concentrated into the panaxynol extracted extract that every milliliter of medicinal liquid contains the 0.1-1g crude drug with filtrate decompression; Remaining medicine residues of Radix Ginseng merges with other raw material of respectively distinguishing the flavor of of making described Chinese medicine composition after will extracting again, decocting with 6-10 times of quality boils 2-3 time, the each decoction 40-60 minute, collecting decoction, filter, filtrate and described panaxynol extracted extract merge, and further are condensed into every milliliter of medicinal liquid again and are equivalent to crude drug 3g, namely get described Chinese medicine composition.
6. arbitrary described Chinese medicine composition according to claim 1-3, it is characterized in that: the preparation method of described Chinese medicine composition, what comprise the steps: to make described Chinese medicine composition respectively distinguishes the flavor of crude drug after 45-55 ℃ of drying, be broken into first coarse powder, be crushed to again fine powder, cross at last sieve 6-9 number, namely get described Chinese medicine composition.
7. arbitrary described Chinese medicine composition according to claim 1-3 is characterized in that: the preparation method of described Chinese medicine composition, comprise the steps: that the Radix Ginseng powder who will make described Chinese medicine composition is broken into fine powder, and stand-by; Other crude drug of respectively distinguishing the flavor of of making described Chinese medicine composition is merged, decocting with 6-10 times of quality boils 2-3 time, the each decoction 40-60 minute, collecting decoction, filter, filtrate decompression is concentrated into every milliliter of medicinal liquid and is equivalent to crude drug 0.2-3g, then adds Radix Ginseng fine powder mixing in concentrated solution, namely gets described Chinese medicine composition.
8. the Chinese medicine preparation of a Cure of depression, its active component is arbitrary described Chinese medicine composition among the claim 1-7.
9. Chinese medicine preparation according to claim 8 is characterized in that: the dosage form of described Chinese medicine preparation be selected from following any one: tablet, dispersible tablet, capsule, soft capsule, granule, pill, micropill, powder, drop pill, decoction, syrup, mixture, oral liquid, soft extract and extractum.
10. the application of arbitrary described Chinese medicine composition in following any one product of preparation among the claim 1-7:
1) preparation anti-depression drug;
2) preparation improves the product that high fat merges monoamine neurotransmitters in the chronic stress depression of sex rat model brain;
3) preparation improves the product that high fat merges chronic stress depression of sex rat model cortex ICAM-1 expression;
4) preparation improves the product of chronic cerebral ischemia depression model rat brain blood flow.
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