CN102233055A - Pharmaceutical composition used for treating cerebral stroke, and preparation method thereof - Google Patents
Pharmaceutical composition used for treating cerebral stroke, and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition, and specifically relates to a pharmaceutical composition used for treating cerebral strokes. The composition is prepared from raw medicines of, by weight: 1 to 10 parts of rhodiola root extract and 1 to 10 parts of figwort root extract. The pharmaceutical composition also can be composed of, by weight: 1 to 10 parts of rhodiola root extract, 1 to 10 parts of figwort root extract, and 1 to 10 parts of salvia miltiorrhiza extract; or the pharmaceutical composition can be composed of, by weight: 1 to 10 parts of rhodiola root extract, 1 to 10 parts of figwort root extract, and 1 to 10 parts of ligusticum wallichii extract; or the pharmaceutical composition can be composed of, by weight: 1 to 10 parts of rhodiola root extract, 1 to 10 parts of figwort root extract, and 1 to 10 parts of lobed kudzuvine root extract. With regular preparation methods in pharmaceutics, the traditional Chinese medicine composition can be prepared into tablets, hard capsules, pills, granules, soft capsules, oral liquid or injections.
Description
Technical field:
The present invention relates to a kind of pharmaceutical composition, be specifically related to a kind of composition and method of making the same with drug extract of treatment apoplexy effect.
Technical background:
It is the disease of main clinical manifestation with brain ischemia and hemorrhagic damage symptom that apoplexy is one group, claim apoplexy or cerebrovascular accident again, have high case fatality rate and disability rate, mainly be divided into hemorrhagic cerebral apoplexy (cerebral hemorrhage or subarachnoid hemorrhage) and ischemia apoplexy (cerebral infarction, cerebral thrombosis) two big classes, the most common with cerebral infarction.Apoplexy morbidity is anxious, and the case fatality rate height is one of most important fatal disease in the world.
The mortality rate of apoplexy also has the trend that rises with age growth, lacks therapy measure for a long time always, and therefore, the medicine of developing safe and effective treatment apoplexy is the problem that current medical educational circles presses for solution.
Summary of the invention:
The purpose of this invention is to provide a kind of pharmaceutical composition for the treatment of apoplexy;
Another object of the present invention provides this preparation of drug combination method;
The objective of the invention is to realize in the following manner:
A kind of pharmaceutical composition for the treatment of apoplexy, said composition is made by comprising following bulk drugs: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug is: Radix Rhodiolae extract 5, Radix Scrophulariae extract 5.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug can also be: Radix Rhodiolae extract 3, Radix Scrophulariae extract 7.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug can also be: Radix Rhodiolae extract 8, Radix Scrophulariae extract 2.
Above preparation of drug combination method is:
((1) gets the Radix Rhodiolae medical material, and 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The optimal technical scheme of above-mentioned preparation method is:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The weight proportion of pharmaceutical composition crude drug of the present invention can also be: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Radix Salviae Miltiorrhizae extract 1～10.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug is: Radix Rhodiolae extract 8, Radix Scrophulariae extract 4, Radix Salviae Miltiorrhizae extract 2.
The preparation method of aforementioned pharmaceutical compositions is:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 70～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution adds ethanol, make determining alcohol reach 75～95%, leave standstill, filter, filtrate is transferred PH to 7～9 with alkaline solution, reclaims ethanol, and concentrated solution is dissolved in water, leave standstill, filter, filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 20～50% ethanol elutions, concentrate, drying promptly gets Radix Salviae Miltiorrhizae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Salviae Miltiorrhizae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The optimal technical scheme of above-mentioned preparation method is:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get red rooted salvia, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Radix Salviae Miltiorrhizae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Salviae Miltiorrhizae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The weight proportion of pharmaceutical composition crude drug of the present invention can also be: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Rhizoma Chuanxiong extract 1～10.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug is: Radix Rhodiolae extract 6, Radix Scrophulariae extract 4, Rhizoma Chuanxiong extract 3.
The preparation method of aforementioned pharmaceutical compositions is:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 70～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 75～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 20～50% ethanol elutions, concentrate, drying promptly gets Rhizoma Chuanxiong extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Rhizoma Chuanxiong extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The optimal technical scheme of above-mentioned preparation method is:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 85%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Rhizoma Chuanxiong extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The weight proportion of pharmaceutical composition crude drug of the present invention can also be: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Radix Puerariae extract 1～10.
The preferred weight proportion of aforementioned pharmaceutical compositions crude drug is: Radix Rhodiolae extract 4, Radix Scrophulariae extract 8, Radix Puerariae extract 2.
The preparation method of aforementioned pharmaceutical compositions is:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 70～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes alcohol amount concentration reach 70～90%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60～80% ethanol elutions, concentrate, drying promptly gets Radix Puerariae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Puerariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
The optimal technical scheme of above-mentioned preparation method is:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used the AB-8 macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Radix Puerariae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Puerariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
Pharmaceutical composition of the present invention has the effect of treatment apoplexy, and the inventor has carried out experimentation to this content, in order to the therapeutical effect of proof pharmaceutical composition of the present invention.Following experiment is used to further specify effect of the present invention, but does not limit the present invention.
Experiment one: to the experimentation of the inaccessible model influence of intraluminal middle cerebral artery occlusion in rats
1. experiment purpose
Make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system, observe the curative effect of pharmaceutical composition of the present invention.
2. test material
70 of SD rats, male, body weight 200 ± 10g is available from Zhejiang Province's Experimental Animal Center.The quality certification number: SCXK (Zhejiang) 2008-0033.
Nitricoxide synthase (NOS) test kit, building up bio-engineering research by Nanjing is provided;
Malonaldehyde is measured test kit (MDA), lot number 20080715;
Superoxide dismutase is measured test kit (SOD), lot number 20080705;
The Coomassie brilliant blue test kit, lot number 20080825;
Above reagent is Nanjing and builds up bio-engineering research institute product.
3. test method
3.1 modeling: make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system.Behind 10% chloral hydrate (0.3ml/100mg) the intraperitoneal injection of anesthesia rat, separate right common carotid artery and cut an osculum, head end is burnt till homemade nylon wire (the diameter 0.205mm of smooth pestle shape, long 6cm) inserts, calculating the inlet wire degree of depth with the common carotid artery crotch is 18mm ± 0.5mm, supplies to block blood fully to the MCA initial part.The sign of model success is that rat occurs right side Horner at once and levies.10 sham operated rats rats are not except that inserting the line bolt, and other operating procedures are the same.
3.2 grouping administration: modeling is after 24 hours, survives rat and according to the method for Longa etc. rat carried out function of nervous system's scoring (standard is seen observation index).Be divided into dosage group (4.5g/kg), embodiment 1 medicine low dose group (2.25g/kg) in sham operated rats (normal saline 2ml/100g), model group (normal saline 2ml/100g), aspirin group (aspirin 0.15g/kg), embodiment 1 medicine high dose group (9g/kg), embodiment 1 medicine at random according to appraisal result.Grouping back gastric infusion, continuous 5 days, administration volume 2ml/100g.
4. observation index
4.1 last administration is after 1 hour, with reference to the method for Longa etc. rat carried out function of nervous system's scoring.0 minute: do not have obvious neurologic impairment; 1 minute: not tensible left side forelimb; 2 minutes: rotate to the left during walking; 3 minutes: topple over to the left during walking; 4 minutes: can not spontaneously walk loss of consciousness.
4.2 with 20% urethane (0.5ml/100g) anesthesia, broken end is got the right side cerebral hemisphere fast.With the ice normal saline of quality (g)/volume (ml), make homogenate with ultrasonic cell disruptor, the centrifugal 15min (1006 * g) of refrigerated centrifuger than adding 0.9% in 1: 9.Get supernatant 0.05ml spectrophotometry NOS activity.
5. experimental result and conclusion
Experimental result see Table 1 with table 2, embodiment 1 medicine high dose, middle dosage, low dose group all can significantly be improved rat model neurological scoring (P＜0.05 or P＜0.01); Three dosage groups of embodiment 1 medicine all can reduce the MDA content in the model cerebral tissue, make the SOD level rising (P＜0.01) of reduction; Dosage, high dose can significantly reduce NOS activity (P＜0.05 or P＜0.01) in embodiment 1 medicine.Experimental result shows that pharmaceutical composition of the present invention has therapeutical effect preferably to the MCAO rat model.
Table 1 embodiment 1 medicine is to the influence of rat function of nervous system scoring
Compare * P＜0.05 #P＜0.01 with model group
Annotate: MDA refers to malonaldehyde, down together.
Table 2 embodiment 1 medicine is to the MDA of rat cerebral tissue, SOD, the active influence of NOS
Compare * P＜0.05 #P＜0.01 with model group
Annotate: SOD refers to superoxide dismutase, down together.
NOS refers to nitricoxide synthase, down together.
Experiment two: to the experimentation of the inaccessible model influence of intraluminal middle cerebral artery occlusion in rats
1. experiment purpose
Make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system, observe the curative effect of pharmaceutical composition of the present invention.
2. test material
70 of SD rats, male, body weight 200 ± 10g, available from Zhejiang Province's Experimental Animal Center, the quality certification number: SCXK (Zhejiang) 2008-0065.
Nitricoxide synthase (NOS) test kit, building up bio-engineering research by Nanjing is provided;
Malonaldehyde is measured test kit (MDA), lot number 20080723;
Superoxide dismutase is measured test kit (SOD), lot number 20080712;
The Coomassie brilliant blue test kit, lot number 20080823;
Above reagent is Nanjing and builds up bio-engineering research institute product.
3. test method
3.1 modeling: make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system.Behind 10% chloral hydrate (0.3ml/100mg) the intraperitoneal injection of anesthesia rat, separate right common carotid artery and cut an osculum, head end is burnt till homemade nylon wire (the diameter 0.205mm of smooth pestle shape, long 6cm) inserts, calculating the inlet wire degree of depth with the common carotid artery crotch is 18mm ± 0.5mm, supplies to block blood fully to the MCA initial part.The sign of model success is that rat occurs right side Horner at once and levies.10 sham operated rats rats are not except that inserting the line bolt, and other operating procedures are the same.
3.2 grouping administration: modeling is after 24 hours, survives rat and according to the method for Longa etc. rat carried out function of nervous system's scoring (standard is seen observation index).Be divided into dosage group (4.5g/kg), embodiment 6 medicine low dose group (2.25g/kg) in sham operated rats (normal saline 2ml/100g), model group (normal saline 2ml/100g), aspirin group (aspirin 0.15g/kg), embodiment 6 medicine high dose group (9g/kg), embodiment 6 medicines at random according to appraisal result.Grouping back gastric infusion, continuous 5 days, administration volume 2ml/100g.
4. observation index
4.1 last administration is after 1 hour, with reference to the method for Longa etc. rat carried out function of nervous system's scoring.0 minute: do not have obvious neurologic impairment; 1 minute: not tensible left side forelimb; 2 minutes: rotate to the left during walking; 3 minutes: topple over to the left during walking; 4 minutes: can not spontaneously walk loss of consciousness.
4.2 with 20% urethane (0.5ml/100g) anesthesia, broken end is got the right side cerebral hemisphere fast.With the ice normal saline of quality (g)/volume (ml), make homogenate with ultrasonic cell disruptor, the centrifugal 15min (1006 * g) of refrigerated centrifuger than adding 0.9% in 1: 9.Get supernatant 0.05ml spectrophotometry NOS activity.
5. experimental result and conclusion
Experimental result see Table 3 with table 4, embodiment 6 medicine high doses, middle dosage, low dose group all can significantly be improved rat model neurological scoring (P＜0.05 or P＜0.01); Three dosage groups of embodiment 6 medicines all can reduce the MDA content in the model cerebral tissue, make the SOD level rising (P＜0.01) of reduction; Dosage, high dose can significantly reduce NOS activity (P＜0.05 or P＜0.01) in embodiment 6 medicines.Experimental result shows that pharmaceutical composition of the present invention has therapeutical effect preferably to the MCAO rat model.
Table 3 embodiment 6 medicines are to the influence of rat function of nervous system scoring
Compare * P＜0.05 #P＜0.01 with model group
Table 4 embodiment 6 medicines are to the MDA of rat cerebral tissue, SOD, the active influence of NOS
Compare * P＜0.05 #P＜0.01 with model group
Experiment three: to the experimentation of the inaccessible model influence of intraluminal middle cerebral artery occlusion in rats
1. experiment purpose
Make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system, observe the curative effect of pharmaceutical composition of the present invention.
2. test material
70 of SD rats, male, body weight 200 ± 10g, available from Zhejiang Province's Experimental Animal Center, the quality certification number: SCXK (Zhejiang) 2008-0045.
Nitricoxide synthase (NOS) test kit, building up bio-engineering research by Nanjing is provided;
Malonaldehyde is measured test kit (MDA), lot number 20080812;
Superoxide dismutase is measured test kit (SOD), lot number 20080823;
The Coomassie brilliant blue test kit, lot number 20080913;
Above reagent is Nanjing and builds up bio-engineering research institute product.
3. test method
3.1 modeling: make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system.Behind 10% chloral hydrate (0.3ml/100mg) the intraperitoneal injection of anesthesia rat, separate right common carotid artery and cut an osculum, head end is burnt till homemade nylon wire (the diameter 0.205mm of smooth pestle shape, long 6cm) inserts, calculating the inlet wire degree of depth with the common carotid artery crotch is 18mm ± 0.5mm, supplies to block blood fully to the MCA initial part.The sign of model success is that rat occurs right side Homer at once and levies.10 sham operated rats rats are not except that inserting the line bolt, and other operating procedures are the same.
3.2 grouping administration: modeling is after 24 hours, survives rat and according to the method for Longa etc. rat carried out function of nervous system's scoring (standard is seen observation index).Be divided into dosage group (4.5g/kg), embodiment 10 medicine low dose group (2.25g/kg) in sham operated rats (normal saline 2ml/100g), model group (normal saline 2ml/100g), aspirin group (aspirin 0.15g/kg), embodiment 10 medicine high dose group (9g/kg), embodiment 10 medicines at random according to appraisal result.Grouping back gastric infusion, continuous 5 days, administration volume 2ml/100g.
4. observation index
4.1 last administration is after 1 hour, with reference to the method for Longa etc. rat carried out function of nervous system's scoring.0 minute: do not have obvious neurologic impairment; 1 minute: not tensible left side forelimb; 2 minutes: rotate to the left during walking; 3 minutes: topple over to the left during walking; 4 minutes: can not spontaneously walk loss of consciousness.
4.2 with 20% urethane (0.5ml/100g) anesthesia, broken end is got the right side cerebral hemisphere fast.With the ice normal saline of quality (g)/volume (ml), make homogenate with ultrasonic cell disruptor, the centrifugal 15min (1006 * g) of refrigerated centrifuger than adding 0.9% in 1: 9.Get supernatant 0.05ml spectrophotometry NOS activity.
5. experimental result and conclusion
Experimental result see Table 5 with table 6, embodiment 10 medicine high doses, middle dosage, low dose group all can significantly be improved rat model neurological scoring (P＜0.05 or P＜0.01); Three dosage groups of embodiment 10 medicines all can reduce the MDA content in the model cerebral tissue, make the SOD level rising (P＜0.01) of reduction; Dosage, high dose can significantly reduce NOS activity (P＜0.05 or P＜0.01) in embodiment 10 medicines.Experimental result shows that pharmaceutical composition of the present invention has therapeutical effect preferably to the MCAO rat model.
Table 5 embodiment 10 medicines are to the influence of rat function of nervous system scoring
Compare * P＜0.05 #P＜0.01 with model group
Table 6 embodiment 10 medicines are to the MDA of rat cerebral tissue, SOD, the active influence of NOS
Compare * P＜0.05 #P＜0.01 with model group
Experiment four: to the experimentation of the inaccessible model influence of intraluminal middle cerebral artery occlusion in rats
1. experiment purpose
Make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system, observe the curative effect of pharmaceutical composition of the present invention.
2. test material
70 of SD rats, male, body weight 200 ± 10g, available from Zhejiang Province's Experimental Animal Center, the quality certification number: SCXK (Zhejiang) 2008-0063.
Nitricoxide synthase (NOS) test kit, building up bio-engineering research by Nanjing is provided;
Malonaldehyde is measured test kit (MDA), lot number 20080917;
Superoxide dismutase is measured test kit (SOD), lot number 20080928;
The Coomassie brilliant blue test kit, lot number 20080916;
Above reagent is Nanjing and builds up bio-engineering research institute product.
3. test method
3.1 modeling: make intraluminal middle cerebral artery occlusion in rats obturation (MCAO) model with line bolt legal system.Behind 10% chloral hydrate (0.3ml/100mg) the intraperitoneal injection of anesthesia rat, separate right common carotid artery and cut an osculum, head end is burnt till homemade nylon wire (the diameter 0.205mm of smooth pestle shape, long 6cm) inserts, calculating the inlet wire degree of depth with the common carotid artery crotch is 18mm ± 0.5mm, supplies to block blood fully to the MCA initial part.The sign of model success is that rat occurs right side Horner at once and levies.10 sham operated rats rats are not except that inserting the line bolt, and other steps are the same.
3.2 grouping administration: modeling is after 24 hours, survives rat and according to the method for Longa etc. rat carried out function of nervous system's scoring (standard is seen observation index).Be divided into dosage group (4.5g/kg), embodiment 14 medicine low dose group (2.25g/kg) in sham operated rats (normal saline 2ml/100g), model group (normal saline 2ml/100g), aspirin group (aspirin 0.15g/kg), embodiment 14 medicine high dose group (9g/kg), embodiment 14 medicines at random according to appraisal result.Grouping back gastric infusion, continuous 5 days, administration volume 2ml/100g.
4. observation index
4.1 last administration is after 1 hour, with reference to the method for Longa etc. rat carried out function of nervous system's scoring.0 minute: do not have obvious neurologic impairment; 1 minute: not tensible left side forelimb; 2 minutes: rotate to the left during walking; 3 minutes: topple over to the left during walking; 4 minutes: can not spontaneously walk loss of consciousness.
4.2 with 20% urethane (0.5ml/100g) anesthesia, broken end is got the right side cerebral hemisphere fast.With the ice normal saline of quality (g)/volume (ml), make homogenate with ultrasonic cell disruptor, the centrifugal 15min (1006 * g) of refrigerated centrifuger than adding 0.9% in 1: 9.Get supernatant 0.05ml spectrophotometry NOS activity.
5. experimental result and conclusion
Experimental result see Table 7 with table 8, embodiment 14 medicine high doses, middle dosage, low dose group all can significantly be improved rat model neurological scoring (P＜0.05 or P＜0.01); Three dosage groups of embodiment 14 medicines all can reduce the MDA content in the model cerebral tissue, make the SOD level rising (P＜0.01) of reduction; Dosage, high dose can significantly reduce NOS activity (P＜0.05 or P＜0.01) in embodiment 14 medicines.Experimental result shows that pharmaceutical composition of the present invention has therapeutical effect preferably to the MCAO rat model.
Table 7 embodiment 14 medicines are to the influence of rat function of nervous system scoring
Compare * P＜0.05 #P＜0.01 with model group
Table 8 embodiment 14 medicines are to the MDA of rat cerebral tissue, SOD, the active influence of NOS
Compare * P＜0.05 #P＜0.01 with model group
Beneficial effect of the present invention:
1. pharmaceutical composition of the present invention is Chinese medicine Radix Scrophulariae extract and the combination of Tibetan medicine Radix Rhodiolae extract aspect the treatment apoplexy, the two compatibility can be worked in coordination with mutually, significantly heightens the effect of a treatment, and produces the effect of 1+1＞2, and do not see untoward reaction, the application prospect that tool is wide.On this basis, the extract of Chinese medicines such as compatibility Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, Radix Puerariae can play certain assosting effect to improving curative effect of medication.
2. the physicochemical property of thing prescription flavour of a drug middle finger mark property composition combined according to the invention, effectiveness composition, by research, preparation method provided by the invention adopts advanced macroporous adsorption resin technology, extract in the Radix Scrophulariae phenethyl alcohol glycosides effective part extract in the plain glycoside effective part extract of iridoid glycoside and phenylpropyl alcohol, the Radix Rhodiolae respectively, active constituent content height, quality controllable, effectively overcome the drawback that the quality instability causes uncertain therapeutic efficacy to be cut, can reduce taking dose simultaneously.
3. the present invention also provides the preparation method of multiple pharmaceutical dosage form, and the present composition is prepared into certain pharmaceutical dosage form can conveniently more be used, and improves therapeutic effect.
Figure of description:
Do not have.
The specific embodiment:
Embodiment 1: hard capsule
Prescription: Radix Rhodiolae extract 500g, Radix Scrophulariae extract 500g
Preparation method: (1) gets the Radix Rhodiolae medical material, 70% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add appropriate amount of starch and (or) dextrin, drying is pulverized, and sieves, and incapsulates, and makes 1000.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 2: hard capsule
Prescription: Radix Rhodiolae extract 300g, Radix Scrophulariae extract 700g
Preparation method: (1) gets the Radix Rhodiolae medical material, and 50% soak with ethanol is extracted 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 55% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add appropriate amount of starch and (or) dextrin, drying is pulverized, and sieves, and incapsulates, and makes 1000.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 3: drop pill
Prescription: Radix Rhodiolae extract 300g, Radix Scrophulariae extract 700g
Preparation method: (1) gets the Radix Rhodiolae medical material, 60% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 60% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, with Polyethylene Glycol-4000 and the ratio melting mixing of intermediate dry powder with 3: 1, methyl-silicone oil is made coolant, drips to make 1000 of drop pill, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 4: injection
Prescription: Radix Rhodiolae extract 600g, Radix Scrophulariae extract 400g
Preparation method: (1) gets the Radix Rhodiolae medical material, and 70% soak with ethanol is extracted 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 65% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get water for injection 1000ml, boil, Radix Rhodiolae extract is mixed the back add with Radix Scrophulariae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, get filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, 100 of injection are sterilized, made to ultrafiltration,, promptly at embedding.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip, every day 1 time, each 10ml～20ml (1～2) instils with 5% glucose injection or 0.9% normal saline solution 250ml dilution posterior vein.
Embodiment 5: powder ampoule agent for injection
Prescription: Radix Rhodiolae extract 400g, Radix Scrophulariae extract 600g
Preparation method: (1) gets the Radix Rhodiolae medical material, 80% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get water for injection 1000ml, boil, Radix Rhodiolae extract is mixed the back add with Radix Scrophulariae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, and got filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, microfiltration, ultrafiltration, lyophilizing, packing is made 100 of powder ampoule agent for injection, promptly.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip.Each 1～2,1 time on the one; Before facing usefulness, instil with sodium chloride injection or 5% glucose injection 500ml dilution posterior vein.
Embodiment 6: granule
Prescription: Radix Rhodiolae extract 800g, Radix Scrophulariae extract 400g, Radix Salviae Miltiorrhizae extract 200g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 70% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 75% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 70% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Salviae Miltiorrhizae extract.
(4) with Radix Rhodiolae extract, Radix Scrophulariae extract mixes with Radix Salviae Miltiorrhizae extract, add appropriate amount of auxiliary materials (as silicon dioxide, dextrin, starch, phosphate, carbonate, mannitol, sorbitol, sorbic acid or potassium salt, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, in dilution such as the mannitol adjuvant one or more), dry, pulverize, add suitable bonding (solid binder such as PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500, microcrystalline Cellulose etc. or liquid adhesive such as ethanol, gelatine size, starch slurry, syrup etc. one or more), granulate, granulate is made granule 5000g, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 10g～20g.
Embodiment 7: tablet
Prescription: Radix Rhodiolae extract 500g, Radix Scrophulariae extract 800g, Radix Salviae Miltiorrhizae extract 100g.
Preparation method: (1) gets the Radix Rhodiolae medical material, and 80% soak with ethanol is extracted 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 80% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 75% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Salviae Miltiorrhizae extract.
(4) with Radix Rhodiolae extract, Radix Scrophulariae extract mixes with Radix Salviae Miltiorrhizae extract, mixing, add appropriate amount of auxiliary materials (as silicon dioxide, dextrin, starch, phosphate, carbonate, mannitol, sorbitol, sorbic acid or potassium salt, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, the dilution adjuvant that mannitol and other pharmaceuticss are commonly used etc. one or more), mix, dry, pulverize, add an amount of binding agent (solid binder such as PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500, the macromolecular material polyvinylpyrrolidone, sodium carboxymethyl cellulose, methylcellulose, low-substituted hydroxypropyl cellulose, hydroxypropyl emthylcellulose, sodium alginate etc. or liquid adhesive such as ethanol, gelatine size, starch slurry etc. one or more), the system granule, add lubricant (as magnesium stearate, Pulvis Talci, sodium stearyl fumarate etc. one or more), granulate, total mixing, be pressed into 1000, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 8: injection
Prescription: Radix Rhodiolae extract 700g, Radix Scrophulariae extract 500g, Radix Salviae Miltiorrhizae extract 300g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 90% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 85% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 65% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 80% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, adding ethanol is 80% to containing the alcohol amount, and cold preservation is left standstill, and filters, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Salviae Miltiorrhizae extract.
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Radix Salviae Miltiorrhizae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, get filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, 100 of injection are sterilized, made to ultrafiltration,, promptly at embedding.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip, every day 1 time, each 10ml～20ml (1～2) instils with 5% glucose injection or 0.9% normal saline solution 250ml dilution posterior vein.
Embodiment 9: powder ampoule agent for injection
Prescription: Radix Rhodiolae extract 500g, Radix Scrophulariae extract 500g, Radix Salviae Miltiorrhizae extract 400g.
Preparation method: (1) gets the Radix Rhodiolae medical material, and 50% soak with ethanol is extracted 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 90% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 85% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Salviae Miltiorrhizae extract.
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Radix Salviae Miltiorrhizae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, and got filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, microfiltration, ultrafiltration, lyophilizing, packing is made 100 of powder ampoule agent for injection, promptly.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip.Each 1～2,1 time on the one; Before facing usefulness, instil with sodium chloride injection or 5% glucose injection 500ml dilution posterior vein.
Embodiment 10: soft capsule
Prescription: Radix Rhodiolae extract 600g, Radix Scrophulariae extract 400g, Rhizoma Chuanxiong extract 300g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 60% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 85% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 70% soak with ethanol is extracted 1 time, reclaims ethanol, is concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 70%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution with 20% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) with Radix Rhodiolae extract, Radix Scrophulariae extract mixes with Rhizoma Chuanxiong extract, add appropriate amount of auxiliary materials (as silicon dioxide, dextrin, starch, phosphate, carbonate, mannitol, sorbitol, sorbic acid or potassium salt, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, in dilution such as the mannitol adjuvant one or more), dry, pulverize, with proper supplementary material (as PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500 and vegetable oil series etc., antiseptic such as sorbic acid or potassium salt, benzoic acid and propanoic acid etc.; Solubilizing agent such as tween series wait one or more, antioxidant such as sodium sulfite, sodium thiosulfate etc. one or more), behind the mixing, in incapsulating, make 1000 soft capsules, promptly by any way.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 11: oral liquid
Prescription: Radix Rhodiolae extract 900g, Radix Scrophulariae extract 500g, Rhizoma Chuanxiong extract 500g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 70% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 80% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 55% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 75% alcohol reflux 1 time reclaims ethanol, is concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 70%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution with 20% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Rhizoma Chuanxiong extract, mixing joins in the boiling water, the heat treatment appropriate time, cold preservation filters, add the agent of the tender flavor of adjuvant as sucrose, Mel, xylitol, stevioside, maltose alcohol etc. one or more; Antiseptic such as sorbic acid or potassium salt, benzoic acid and propanoic acid etc. one or more; Solubilizing agent such as tween series etc., antioxidant such as sodium sulfite, sodium thiosulfate or the like one or more), filter, 10000mL is made in embedding, sterilization, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 10mL～20mL.
Embodiment 12: injection
Prescription: Radix Rhodiolae extract 600g, Radix Scrophulariae extract 400g, Rhizoma Chuanxiong extract 300g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 80% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 75% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 80% soak with ethanol is extracted 1 time, reclaims ethanol, is concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 85%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution with 35% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Rhizoma Chuanxiong extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, get filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, 100 of injection are sterilized, made to ultrafiltration,, promptly at embedding.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip, every day 1 time, each 10ml～20ml (1～2) instils with 5% glucose injection or 0.9% normal saline solution 250ml dilution posterior vein.
Embodiment 13: powder ampoule agent for injection
Prescription: Radix Rhodiolae extract 800g, Radix Scrophulariae extract 500g, Rhizoma Chuanxiong extract 100g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 90% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 90% alcohol reflux extracts 3 times, reclaims ethanol, is concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 85%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution with 30% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Rhizoma Chuanxiong extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, and got filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, microfiltration, ultrafiltration, lyophilizing, packing is made 100 of powder ampoule agent for injection, promptly.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip.Each 1～2,1 time on the one; Before facing usefulness, instil with sodium chloride injection or 5% glucose injection 500ml dilution posterior vein.
Embodiment 14: concentrated pill
Prescription: Radix Rhodiolae extract 400g, Radix Scrophulariae extract 800g, Radix Puerariae extract 200g.
Preparation method: (1) gets the Radix Rhodiolae medical material, and 75% soaks reflux, extract, 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 65% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 70% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Puerariae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Puerariae extract, mixing adds suitable quantity of water, the general ball of making, and cold drying is made 5000 of concentrated pills, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 10～20.
Embodiment 15: chewable tablet
Prescription: Radix Rhodiolae extract 200g, Radix Scrophulariae extract 600g, Radix Puerariae extract 400g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 65% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 60% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 65% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 80% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Puerariae extract;
(4) with Radix Rhodiolae extract, Radix Scrophulariae extract mixes with Radix Puerariae extract, mixing, add appropriate amount of auxiliary materials (as silicon dioxide, dextrin, starch, phosphate, carbonate, mannitol, sorbitol, sorbic acid or potassium salt, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, in dilution such as lactose or the mannitol adjuvant one or more) mix, dry, pulverize, obtain fine powder and an amount of binding agent (solid binder such as PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500, the macromolecular material polyvinylpyrrolidone, sodium carboxymethyl cellulose, methylcellulose, low-substituted hydroxypropyl cellulose, hydroxypropyl emthylcellulose, sodium alginate or the like or liquid adhesive such as ethanol, starch slurry, the dextrin slurry waits one or more) mixed granule, add an amount of correctives (as mannitol, sucrose, xylitol, stevioside, maltose alcohol, Mentholum, erythrol, orange peel oil, Oleum Cinnamomi, various essence etc. one or more), lubricant is (as magnesium stearate, Pulvis Talci, sodium stearyl fumarate, magnesium laurylsulfate etc. one or more), disintegrating agent is (as carboxymethyl starch sodium, cross-linking sodium carboxymethyl cellulose, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, pregelatinized Starch, microcrystalline Cellulose, soybean polysaccharide, alginic acid, handle agar etc. one or more) granulate, total mixing, be pressed into 1000, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 16: injection
Prescription: Radix Rhodiolae extract 400g, Radix Scrophulariae extract 800g, Radix Puerariae extract 200g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 55% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 85% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Puerariae extract;
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Radix Puerariae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, get filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, 100 of injection are sterilized, made to ultrafiltration,, promptly at embedding.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip, every day 1 time, each 10ml～20ml (1～2) instils with 5% glucose injection or 0.9% normal saline solution 250ml dilution posterior vein.
Embodiment 17: powder ampoule agent for injection
Prescription: Radix Rhodiolae extract 400g, Radix Scrophulariae extract 800g, Radix Puerariae extract 200g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 85% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 80% alcohol reflux 2 times each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 55% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 90% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used the AB-8 macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Puerariae extract;
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Radix Puerariae extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, and got filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, microfiltration, ultrafiltration, lyophilizing, packing is made 100 of powder ampoule agent for injection, promptly.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip.Each 1～2,1 time on the one; Before facing usefulness, instil with sodium chloride injection or 5% glucose injection 500ml dilution posterior vein.
Embodiment 18: injection
Prescription: Radix Rhodiolae extract 700g, Radix Scrophulariae extract 500g, Radix Astragali extract 300g.
Preparation method: (1) gets the Radix Rhodiolae medical material, and 90% ethyl acetate is soaked and extracted each 2 hours 2 times, reclaim ethyl acetate, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is used silica gel adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 85% ethyl acetate is soaked and is extracted 2 times, each 2 hours, reclaims ethyl acetate, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used silica gel adsorption, behind water elution,, collect ethanol elution again with 65% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get Milkvetch Root, 80% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, adding ethanol is 80% to containing the alcohol amount, and cold preservation is left standstill, and filters, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used silica gel adsorption, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Astragali extract.
(4) get water for injection 1000ml, boil, Radix Rhodiolae extract, Radix Scrophulariae extract are mixed the back add with Radix Astragali extract, boiled 15 minutes, cold preservation was not less than 48 hours, filtered, get filtrate, transfer pH, add 0.5% active carbon after boiling and boil, after the absorption, filter, get filtrate, be chilled to room temperature, 100 of injection are sterilized, made to ultrafiltration,, promptly at embedding.
Cure mainly: apoplexy.
Usage and dosage: intravenous drip, every day 1 time, each 10ml～20ml (1～2) instils with 5% glucose injection or 0.9% normal saline solution 250ml dilution posterior vein.
Embodiment 19: soft capsule
Prescription: Radix Rhodiolae extract 600g, Radix Scrophulariae extract 400g, Radix Angelicae Sinensis extract 300g.
Preparation method: (1) gets the Radix Rhodiolae medical material, 60% alcohol reflux 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is used alumina adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 85% soak with ethanol is extracted 1 time, each 3 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used alumina adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Angelicae Sinensis medical material, 70% ethyl acetate is soaked and is extracted 1 time, reclaims ethyl acetate, is concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 70%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used alumina adsorption, behind water elution,, collect ethanol elution with 20% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Radix Angelicae Sinensis extract;
(4) with Radix Rhodiolae extract, Radix Scrophulariae extract mixes with Radix Angelicae Sinensis extract, add appropriate amount of auxiliary materials (as silicon dioxide, dextrin, starch, phosphate, carbonate, mannitol, sorbitol, sorbic acid or potassium salt, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, in dilution such as the mannitol adjuvant one or more), dry, pulverize, with proper supplementary material (as PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500 and vegetable oil series etc., antiseptic such as sorbic acid or potassium salt, benzoic acid and propanoic acid etc.; Solubilizing agent such as tween series wait one or more, antioxidant such as sodium sulfite, sodium thiosulfate etc. one or more), behind the mixing, in incapsulating, make 1000 soft capsules, promptly by any way.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 2～4.
Embodiment 20: concentrated pill
Prescription: Radix Rhodiolae extract 400g, Radix Scrophulariae extract 800g, Radix Paeoniae Rubra extract 200g.
Preparation method: (1) gets the Radix Rhodiolae medical material, and 75% soaks reflux, extract, 2 times, each 2 hours, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 65% soak with ethanol is extracted 2 times, each 2 hours, reclaims ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Paeoniae Rubra medical material, 70% ethyl acetate backflow is extracted 2 times, each 2 hours, reclaims ethyl acetate, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum drying promptly gets Radix Paeoniae Rubra extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Paeoniae Rubra extract, mixing adds suitable quantity of water, the general ball of making, and cold drying is made 5000 of concentrated pills, promptly.
Cure mainly: apoplexy.
Usage and dosage: oral, every day 2～3 times, each 10～20.
Claims (18)
1. pharmaceutical composition for the treatment of apoplexy is characterized in that said composition makes by comprising following bulk drugs: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10.
2. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 5, Radix Scrophulariae extract 5.
3. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 3, Radix Scrophulariae extract 7.
4. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 8, Radix Scrophulariae extract 2.
5. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Radix Salviae Miltiorrhizae extract 1～10.
6. 5 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 8, Radix Scrophulariae extract 4, Radix Salviae Miltiorrhizae extract 2.
7. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Rhizoma Chuanxiong extract 1～10.
8. 7 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 6, Radix Scrophulariae extract 4, Rhizoma Chuanxiong extract 3.
9. 1 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 1～10, Radix Scrophulariae extract 1～10, Radix Puerariae extract 1～10.
10. 9 pharmaceutical composition as claimed in claim is characterized in that the weight proportion of said composition crude drug is as follows: Radix Rhodiolae extract 4, Radix Scrophulariae extract 8, Radix Puerariae extract 2.
11., it is characterized in that preparation method is as the described pharmaceutical composition of claim 2～4:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
12. preparation method as claimed in claim 11 is characterized in that:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, extract 2 times, each 2 hours, reclaim ethanol with 70% alcohol reflux or immersion, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) Radix Rhodiolae extract is mixed with Radix Scrophulariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
13., it is characterized in that preparation method is as natural drug composition as described in the claim 5～6:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get red rooted salvia, 70～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution adds ethanol, make determining alcohol reach 75～95%, leave standstill, filter, filtrate is transferred PH to 7～9 with alkaline solution, reclaims ethanol, and concentrated solution is dissolved in water, leave standstill, filter, filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 20～50% ethanol elutions, concentrate, drying promptly gets Radix Salviae Miltiorrhizae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Salviae Miltiorrhizae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
14. preparation method as claimed in claim 13 is characterized in that:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get red rooted salvia, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate is transferred PH to 8 with the sodium hydroxide alcoholic solution, reclaims ethanol, and concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is adsorbed with polyamide, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Radix Salviae Miltiorrhizae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Salviae Miltiorrhizae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
15., it is characterized in that preparation method is as natural drug composition as described in the claim 7～8:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 70～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 75～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 20～50% ethanol elutions, concentrate, drying promptly gets Rhizoma Chuanxiong extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Rhizoma Chuanxiong extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
16. preparation method as claimed in claim 15 is characterized in that:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get the Rhizoma Chuanxiong medical material, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 85%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 35% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Rhizoma Chuanxiong extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Rhizoma Chuanxiong extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
17., it is characterized in that preparation method is as natural drug composition as described in the claim 9～10:
(1) get the Radix Rhodiolae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, and concentrated solution is dissolved in water, and leaves standstill, filter, filtrate is used macroporous resin adsorption, behind water elution, again with 20～50% ethanol elutions, collect ethanol elution, eluent concentrates, and drying promptly gets Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 50～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes determining alcohol reach 70～95%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 30～70% ethanol elutions, eluent concentrates, and drying promptly gets Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 70～90% ethanol extractions 1～3 time reclaim ethanol, concentrated solution adds ethanol, makes alcohol amount concentration reach 70～90%, leaves standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, leave standstill, filter, filtrate is used macroporous resin adsorption, behind water elution,, collect ethanol elution again with 60～80% ethanol elutions, concentrate, drying promptly gets Radix Puerariae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Puerariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
18. preparation method as claimed in claim 17 is characterized in that:
(1) get the Radix Rhodiolae medical material, 70% alcohol reflux or immersion are extracted each 2 hours 2 times, reclaim ethanol, concentrated solution is dissolved in water, and cold preservation is left standstill, filter, filtrate is with AB-8 type macroporous resin adsorption, behind water elution, again with 40% ethanol elution, collect ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Rhodiolae extract;
(2) get the Radix Scrophulariae medical material, 70% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 75%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used AB-8 type macroporous resin adsorption, behind water elution,, collect ethanol elution again with 50% ethanol elution, the eluent concentrating under reduced pressure, vacuum or spray drying promptly get Radix Scrophulariae extract;
(3) get the Radix Puerariae medical material, 80% alcohol reflux or immersion are extracted 2 times, each 2 hours, reclaim ethanol, be concentrated into 1: 1, concentrated solution adds ethanol, makes determining alcohol reach 80%, and cold preservation is left standstill, filter, filtrate recycling ethanol, concentrated solution is dissolved in water, cold preservation is left standstill, and filters, and filtrate is used the AB-8 macroporous resin adsorption, behind water elution,, collect ethanol elution again with 70% ethanol elution, concentrating under reduced pressure, vacuum or spray drying promptly get Radix Puerariae extract;
(4) Radix Rhodiolae extract, Radix Scrophulariae extract are mixed with Radix Puerariae extract, add conventional pharmaceutic adjuvant, adopt pharmaceutical methods conventional in the pharmaceutics to be prepared into tablet, hard capsule, pill, granule, soft capsule, oral liquid, injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN109452708A (en) * | 2018-11-24 | 2019-03-12 | 广西中医药大学 | A kind of method of preparation and use of graphene lumbar medicinal waistband |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106674A (en) * | 1994-10-13 | 1995-08-16 | 汪志伟 | Traditional Chinese medicine for curing paralysis |
| CN101129817A (en) * | 2007-09-26 | 2008-02-27 | 王福春 | Chinese medicine formulation for treating hypertension and method of producing the same |
| CN101690795A (en) * | 2009-09-25 | 2010-04-07 | 威海东宝制药有限公司 | Tianshen vessel-freeing granules for treating cardiovascular and cerebrovascular diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106674A (en) * | 1994-10-13 | 1995-08-16 | 汪志伟 | Traditional Chinese medicine for curing paralysis |
| CN101129817A (en) * | 2007-09-26 | 2008-02-27 | 王福春 | Chinese medicine formulation for treating hypertension and method of producing the same |
| CN101690795A (en) * | 2009-09-25 | 2010-04-07 | 威海东宝制药有限公司 | Tianshen vessel-freeing granules for treating cardiovascular and cerebrovascular diseases |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452708A (en) * | 2018-11-24 | 2019-03-12 | 广西中医药大学 | A kind of method of preparation and use of graphene lumbar medicinal waistband |
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