CN102228574A - Preparation method of root of Japanese banana extract and product and application thereof - Google Patents
Preparation method of root of Japanese banana extract and product and application thereof Download PDFInfo
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Abstract
The invention discloses a preparation method of root of Japanese banana extract and a product and application thereof. The preparation method comprises the steps of: slicing fresh root of Japanese banana, smashing, juicing by a juicer, extracting the juice by normal butanol, concentrating by pressure reduction, and drying to obtain an extract X; or dissolving the extract extracted by the normal butanol in water, loading on macroporous absorbent resin columns, respectively eluting with water and 30%-70% ethanol of which the volumes are 2-7 times of that of the columns, discarding the water eluent, collecting the ethanol eluent, concentrating by pressure reduction, and drying to obtain an extract Y. In the invention, the extract with hpyerglycemic effect (activity) is extracted from the root of Japanese banana, the extract has definite components, obvious pharmacological activity, stable nature, safety and reliability, and the extract can be used as a main component for processing a suitable pharmaceutical preparation for treating diabetes, and the pharmaceutical preparation has obvious curative effect proven by pharmacodynamics; and the raw material of the extract is easy to obtain, and the preparation process is easy to industrialize, thus promoting the full development and utilization of the medicinal resources in minority areas.
Description
Technical field
The present invention relates to the preparation method and products thereof of Radix Musae extract and use, belong to the Chinese medicine extract technical field.
Background technology
Diabetes be a kind of because defect of insulin secretion and (or) its biological action obstacle causes is the metabolic disease of feature with the hyperglycemia.This disease is only second to tumor and cardiovascular and cerebrovascular disease, is listed in the big disease in third place in the world.
At present, the whole world has 300,000,000 6 thousand ten thousand people to suffer from type ii diabetes approximately, accounts for 6% of the whole world 6,000,000,000 populations, just has a people to suffer from diabetes in per approximately 5 seconds, a people is just arranged because of diabetes death in per 10 seconds.China has become one of fastest-rising country of global diabetes prevalence.National nutrition in 2002 and investigation of health conditions show that China has diabetics more than 2,000 ten thousand approximately, and other has nearly 2,000 ten thousand impaired glucose tolerance persons.The China's 14 provinces and cities' diabetes and the metabolism syndrome survey result of being carried out by diabetology branch of Chinese Medical Association of issue in 2010 show that in the part big city, the diabetes prevalence level has reached 10%.Diabetes need lifelong monitoring and treatment, if can not obtain good control, blood capillary that hyperglycemia is brought and trunk complication, to cause severe complications such as patient's secondary cardiovascular disease, dyslipidemia, blind, apoplexy, renal failure and amputation, serious harm people's health and lives.
The medicine that is used for the treatment of at present diabetes clinically mainly contains insulin, insulin analog, sulfonylurea, biguanides, thiazolidinediones etc., but there are certain limitation and untoward reaction more, as weight increase, cause hypoglycemia, heart failure, lactic acidosis, life-time service leads to complications etc.And traditional insulin blood sugar control can not reach ideal effect.Insulin is mechanical blood sugar lowering, even can reduce to zero to blood glucose.Even popular insulin pump is arranged now, can not tackle the problem at its root.Therefore, from the Chinese medicine that constantly highlights of unique effect with treatment chronic disease, commonly encountered diseases and difficult miscellaneous diseases and ethnic drug, develop the urgent needs that safe and effective original anti-diabetic new drug is domestic and international medical market and society.
The Miao Ethnomedicine Radix Musae that records of version " Guizhou Province's Chinese crude drug, national quality of medicinal material standard " was a Musaceae plant Musa basjoo Sieb. Et Zucc. Musa bas joo Sieb.et Zucc. rhizome in 2003, has heat-clearing and toxic substances removing, the quench the thirst effect of diuresis cures mainly a day capable calentura, unhappiness, quenches one's thirst, jaundice, edema etc.Be called the loud, high-pitched sound brain in Guizhou Province southeast Miao ethnic group area and repair, all there is cultivation on each Miao Xiang Dong stockaded village, the conventional crude drugs that is used for heat-clearing and toxic substances removing, quenches the thirst for Miao ethnic group area.The inventor discovers Miao Ethnomedicine Radix Musae function and this agrostology that cures mainly, its treatment diabetes is with a long history, controls diabetes (diabetes) with regard to its bright product on the books in " Japan hanako materia medica ", Compendium of Material Medica, " Bencao Congxin ", Holy Benevolent Prescriptions, " Zhejiang medicinal plants will " and successive dynasties books on Chinese herbal medicine such as " Chinese Plants will ".Present rarely seen Guizhou pharmacy corporation has been developed preparations such as " bone recovering capsule ", " easypro spray swells and ache " with Miao Ethnomedicine Radix Musae and other medical material compatibilities, is mainly used in treatment fracture, osteoporosis, osteoarthritis etc.Radix Musae contains saponin, polysaccharide, volatile oil and a spot of coumarin, flavone compound, Zhang Qian etc. obtain chemical compound from the separation of Radix Musae ethyl acetate position: 2 ', 3,4 '-trihydroxyflavone, 3,3 '-bis-hydroxyanigorufone, irenolone, 2,4-dihydroxy-9-(4 '-hydroxyphenyl)-and phenalen-one and 3, the 4-4-dihydroxy benzaldehyde.Wherein chemical compound 2 ', 3,4 '-trihydroxyflavone, irenolone and 3, the 4-4-dihydroxy benzaldehyde has antioxidation activity in vitro.3,3 '-bis-hydroxyanigorufone and 2,4-dihydroxy-9-(4 '-hydroxyphenyl)-phenalen-one has external alpha-glucosidase and suppresses active.
Report Radix Musae petroleum ether such as Qian Haibing and n-butanol extract can obviously alleviate the mice auricle swelling due to the dimethylbenzene and the weight of granuloma induced by implantation of cotton pellets, and the mice foot swelling due to the on Carrageenan also has the obvious suppression effect; Radix Musae ethanol extraction and water extract Dichlorodiphenyl Acetate induced mice writhing response have good inhibitory effect.Employing disk diffusion methods such as Wei Jinfeng are measured the minimum inhibitory concentration (MIC) of different extract parts and are studied the bacteriostatic activity of the different extract parts with Flos Musae of Radix Musae to staphylococcus aureus (SA), methicillin resistant staphylococcus aureus (MRSA) and the male staphylococcus aureus of beta-lactamase (ESBLs), and the bacteriostatic activity size order is Radix Musae petroleum ether part＞Flos Musae petroleum ether part＞Radix Musae n-butanol portion.
But the effect of the bright product treatment of Radix Musae diabetes is concrete relevant with which composition or which position, still is not very clear at present, therefore is necessary to further investigate the bright product anti-diabetic of Radix Musae effective site, thereby instructs the more significant new drug preparation of exploitation curative effect.
Summary of the invention
The objective of the invention is to: the preparation method and products thereof and the application in pharmacy of Radix Musae extract are provided.The extract component that adopts the inventive method to obtain is clear and definite, and pharmacologically active is obvious, and stable performance, safe and reliable can be used for preparing the medicine for the treatment of diabetes, and it is evident in efficacy.
Technical scheme of the present invention: the preparation method of Radix Musae extract may further comprise the steps:
A, with aquatic foods product Radix Musae section, smash to pieces, squeeze the juice with juice extractor, the juice n-butanol extraction, concentrating under reduced pressure, drying must extract X;
B, with steps A gained n-butanol extraction extractum water dissolution, last macroporous adsorptive resins is used water, 30%～70% ethanol elution of 2～7 times of column volumes respectively, discards water elution liquid, collects ethanol elution, concentrating under reduced pressure, drying, extract Y.
The preparation method of preferred Radix Musae extract may further comprise the steps:
A, with aquatic foods product Radix Musae section, smash to pieces, extract 4～6 times with 0～95% soak with ethanol earlier, again residue is squeezed the juice merging alcohol extract and squeezeding juice, being evaporated to does not have the alcohol flavor, directly with aqueous dispersion or dry back aqueous dispersion, reuse n-butanol extraction, concentrating under reduced pressure, drying gets extract X;
B, with steps A gained n-butanol extraction extractum water dissolution, last macroporous adsorptive resins is used water, 50% ethanol elution of 2～7 times of column volumes respectively, discards water elution liquid, collects ethanol elution, concentrating under reduced pressure, drying, extract Y.
Concentrating under reduced pressure described in abovementioned steps A and the step B carries out under 25 ℃～75 ℃ conditions, and described drying is carried out under 25 ℃～75 ℃ or-25 ℃～-55 ℃ conditions.
Dry vacuum decompression drying, constant pressure and dry, lyophilization or the spray drying of adopting described in abovementioned steps A and the step B.
Soak with ethanol extraction temperature is 10 ℃～75 ℃ among the abovementioned steps A.
Extract X that the preparation method of aforementioned Radix Musae extract prepares or extract Y.
Aforesaid Radix Musae extract X or the extract Y application in the medicine of preparation treatment diabetes.
In order to verify the active effect of Radix Musae extract of the present invention, the inventor has carried out relevant experimental study to it, and is specific as follows:
One, extract qualitative identification and assay
Through feature chromogenic reaction, thin layer chromatography and ultraviolet detection, 30% ethanol (extract Y-1), 50% ethanol (extract Y-2), the 70% ethanol elution thing (extract Y-3) of bright product Radix Musae mainly contain saponin and phenols component.
Set up total saponins and total phenols component content in ultraviolet-spectrophotometry Radix Musae 30%～70% ethanol elution thing (extract Y):
With vanillin-glacial acetic acid-perchloric acid development process, under 543nm, measure ultraviolet absorption value, with oleanolic acid product in contrast, sample does not add developer for blank.
Dodecyl sodium sulfate-potassium ferricyanide-ferric chloride development process is measured ultraviolet absorption value under 745nm, with gallic acid product in contrast, sample does not add developer for blank.
Result: the quality (total saponins and total phenols component content summation 50%～70%) that can detect, monitor Radix Musae 30%～70% ethanol elution thing with ultraviolet-spectrophotography.
Two, Radix Musae squeezeding juice different parts extract is to the influence of blood glucose in diabetic mice
1, supplies the preparation of reagent liquid
Get Radix Musae (directly squeezing the juice) squeezeding juice without ethanol extraction, use petroleum ether (60～90 ℃), ethyl acetate and n-butanol extraction successively, each extract of concentrating under reduced pressure obtains petroleum ether extractum (A) respectively, ethyl acetate extractum (B), n-butyl alcohol extractum (C, be equivalent to extract X).
1.1 the preparation of squeezeding juice petroleum ether part high and low dose group medicinal liquid
It is an amount of to get petroleum ether extractum (A), adds a small amount of tween 80 and grinds hydrotropy in pure water, is configured to the ligroin extraction solution of 10mg/ml.High dose group is pressed the administration of 0.2ml/10g volume; Low dose group with behind one times of the high dose solution dilution by the equal volume administration.
1.2 the preparation of squeezeding juice ethyl acetate extract high and low dose group medicinal liquid
It is an amount of to get ethyl acetate extractum (B), adds a small amount of tween 80 and grinds hydrotropy in pure water, is configured to the ethyl acetate extract solution of 10mg/ml.High dose group is pressed the 0.2ml/10g administration; Low dose group with behind one times of the high dose solution dilution by the equal volume administration.
1.3 the preparation of squeezeding juice n-butanol portion high and low dose group medicinal liquid
It is an amount of to get n-butyl alcohol extractum (C), adds a small amount of tween 80 and grinds hydrotropy in pure water, is configured to the n-butanol extract solution of 10mg/ml.High dose group is pressed the 0.2ml/10g administration; Low dose group with behind one times of the high dose solution dilution by the equal volume administration.
2, the different parts extract is to the influence of alloxan diabetes mouse blood sugar
2.1 model copy: Kunming kind white mice, age in 6-8 week, body weight 18-22g, male, after water 12h was can't help in the mice fasting, tail vein was got in docking, measured fasting blood sugar with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, as blood glucose before the modeling.Blood glucose value is all within normal range.Show that mice basis blood glucose is qualified, get 10 mices at random and be decided to be the blank group.After remaining mice fasting be can't help water 12h, lumbar injection 200mg/kg alloxan normal saline solution, normal feeding feedwater.After that night, water 12h was can't help in fasting once more, lumbar injection 200mg/kg alloxan normal saline solution, normal feeding feedwater.Behind the normal nursing 72h, the tail vein is got blood, measures fasting glucose (water 12h is can't help in fasting before measuring) with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, and surpassing 10.0mmol person with blood glucose value is that modeling is successful.
2.2 80 of the mices of grouping and administration modeling success are divided into 8 groups, 10 every group at random; Be respectively model group, Radix Musae squeezeding juice petroleum ether part (A) high dose group, Radix Musae squeezeding juice petroleum ether part (A) low dose group, Radix Musae squeezeding juice ethyl acetate extract (B) high dose group, Radix Musae squeezeding juice ethyl acetate extract (B) low dose group, Radix Musae squeezeding juice n-butanol portion (C) high dose group, Radix Musae squeezeding juice n-butanol portion (C) low dose group, metformin positive controls.Add 10 of blank groups, totally 9 groups.Respectively 2 weeks of administration (blank group and model group give the pure water of equal volume), after water 12h was can't help in fasting, the tail vein was got blood and is surveyed the mice fasting glucose with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson.The results are shown in Table 1.
Table 1 squeezeding juice different parts extract is to the influence of alloxan diabetes mouse blood sugar
Annotate: * and model group compare, P＜0.05, and * * and model group compare, P＜0.01.
By table 1 as can be seen, compare with model control group, Radix Musae squeezeding juice n-butanol portion (C, be equivalent to extract X) high and low dose group obviously reduces the blood glucose value level of mice, effect with obvious blood sugar lowering, and Radix Musae squeezeding juice petroleum ether part (A) high and low dose group, Radix Musae squeezeding juice ethyl acetate extract (B) high and low dose group reduces not obvious to the blood glucose value level of mice.So the present invention selects for use n-butyl alcohol as solvent the Radix Musae squeezeding juice to be extracted.
Three, bright product Radix Musae alcohol is mentioned the difference extraction extractive part hypoglycemic activity evaluation study of squeezeding juice
Get the mixed liquor of bright product Radix Musae alcohol extract and squeezeding juice, use aqueous dispersion behind the concentrating under reduced pressure, use petroleum ether, ethyl acetate, n-butanol extraction successively, each extract of concentrating under reduced pressure gets petroleum ether extract (D), acetic acid ethyl ester extract (E), n-butyl alcohol extract (F, be equivalent to extract X), press the dosage of 200mg/kg, administration volume 0.2ml/10g, being mixed with concentration is the 10mg/ml aqueous solution; High dose is pressed 0.2ml/10g (the weight of animals) administration, low dosage with behind one times of the high dose solution dilution by with the volume administration.Petroleum ether and acetic acid ethyl ester extract add tween 80 and grind hydrotropy in distilled water.The preparation of positive drug: get three metformin (0.25g/ sheet), grind into powder, the distilled water that adds 25ml is mixed with the solution that concentration is 0.03g/ml, 0.1ml/10g administration volume.
1. modeling method
Kunming mouse, 6～8 ages in week, body weight 18～22g, male, after water 12h was can't help in the mice fasting, the tail vein was got blood, measured fasting blood sugar with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, as blood glucose before the modeling.Blood glucose value is all within range of normal value as a result; Getting 10 mices at random is the blank group.After remaining mice fasting be can't help water 12h, lumbar injection dosage was 200mg/kg alloxan normal saline solution, normal feeding feedwater.After that night, water 12h was can't help in fasting once more, lumbar injection dosage was 200mg/kg alloxan normal saline solution, normal feeding feedwater.Behind the normal nursing 72h, the tail vein is got blood, measures fasting glucose (water 12h is can't help in fasting before measuring) with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, and surpassing 10.0mmol person with blood glucose value is that modeling is successful.
2. animal grouping, administration and result
Get 90 of the mices of modeling success, be divided into 9 groups at random, 10 every group; Be respectively 10 of model group, 10 of blank groups, 10 of metformin positive drug groups, 10 of petroleum ether (D) high dose group, 10 of petroleum ether (D) low dose group, 10 of ethyl acetate (E) high dose group, 10 of ethyl acetate (E) low dose group, 10 of n-butyl alcohol (F) high dose group, 10 of n-butyl alcohol (F) low dose group, difference two weeks (model group and blank group give the distilled water of equal volume), water 12h is can't help in fasting after the last administration, the tail vein is got blood, surveys the mice fasting blood sugar with blood glucose meter.The results are shown in Table 2.
The bright product Radix Musae of table 2 alcohol is mentioned the influence of the different extracts of squeezeding juice to the alloxan diabetes mouse blood sugar
Annotate: * and model group compare, P＜0.05; * and model group compare, P＜0.01.
As shown in Table 2, compare with model group, the high low dose group of n-butyl alcohol extract (F, be equivalent to extract X) has the effect of obvious blood sugar lowering, and acetic acid ethyl ester extract (E) has blood sugar lowering trend, and the high low dose group of petroleum ether extract (D) DeGrain aspect blood sugar lowering.
Four, the macroporous adsorbent resin eluate hypoglycemic activity evaluation study of bright product Radix Musae
It is an amount of to get the macroporous adsorbent resin eluate respectively, presses the dosage of 200mg/kg, administration volume 0.2ml/10g, and being mixed with concentration is the 10mg/ml aqueous solution; High dose is pressed 0.2ml/10g (the weight of animals) administration, low dosage with behind one times of the high dose solution dilution by with the volume administration.Wherein 70% ethanol elution thing adds tween 80 grinding hydrotropy.The preparation of positive drug: get three metformin (0.25g/ sheet), grind into powder, the distilled water that adds 25ml is mixed with the solution that concentration is 0.03g/ml, 0.1ml/10g administration volume.
1. modeling method
Kunming mouse, 6～8 ages in week, body weight 18～22g, male, after water 12h was can't help in the mice fasting, the tail vein was got blood, measured fasting blood sugar with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, as blood glucose before the modeling.Blood glucose value is all within range of normal value as a result; Getting 10 mices at random is the blank group.After remaining mice fasting be can't help water 12h, lumbar injection dosage was 200mg/kg alloxan normal saline solution, normal feeding feedwater.After that night, water 12h was can't help in fasting once more, lumbar injection dosage was 200mg/kg alloxan normal saline solution, normal feeding feedwater.Behind the normal nursing 72h, the tail vein is got blood, measures fasting glucose (water 12h is can't help in fasting before measuring) with the steady bold and unconstrained blood glucose meter of Johnson ﹠ Johnson, and surpassing 10.0mmol person with blood glucose value is that modeling is successful.
2. animal grouping, administration and result
Get 80 of the mices of modeling success, be divided into 10 of model control group, 10 of blank groups, 10 of metformin positive controls, each 10 of the high low dose group of 30% ethanol elution thing (extract Y-1), each 10 of the high low dose group of 50% ethanol elution thing (extract Y-2), 10 of 70% ethanol elution thing (extract Y-3) high dose group at random, difference two weeks (model group and blank group give the distilled water of equal volume), water 12h is can't help in fasting after the last administration, the tail vein is got blood, surveys the mice fasting blood sugar with blood glucose meter.The results are shown in Table 3.
Each eluate of table 3 macroporous adsorbent resin is to the influence of alloxan diabetes mouse blood sugar
* compare P＜0.05 with model group; * and model group compare, P＜0.01.
Can draw by table 3, compare with model group, the hypoglycemic activity of the high low dose group of 30% ethanol elution thing (being extract Y-1), 50% ethanol elution thing (being extract Y-2) high low dose group and 70% ethanol elution thing (being extract Y-3) high dose group is comparatively obvious, and is wherein obvious with 50% ethanol elution object height low dose group blood sugar decreasing effect.
Five, bright product Radix Musae 50% ethanol elution thing is to the influence of normal mouse carbohydrate tolerance
Get 20 of normal mouses, after water 12h is can't help in fasting, survey its blood glucose value, be divided into two groups, 10 every group: blank group and administration group according to blood glucose value; Administration group successive administration 8d, after the last administration again fasting can't help water 12h, detect blood glucose at 0min, 30min, 60min, 120min respectively after giving D/W.See Table 4.
Table 450% ethanol elution thing is to the influence of normal mouse carbohydrate tolerance
By comparing normal mouse blank group and normal mouse administration group 0min, 30min, 60min, the 120min blood glucose value after giving glucose respectively, the result shows that bright product Radix Musae 50% ethanol elution things (being extract Y-2) are influential to the carbohydrate tolerance of normal mouse, can increase the utilization of normal mouse to sugar.
Compared with prior art, the present invention adopts the modern times at body medicine efficacy screening technology, extraction and separation technology and Quality Control Technology the Miao Ethnomedicine Radix Musae extract to be carried out deep development, from resourceful Miao ethnic group medical material Radix Musae, extract the have hypoglycemic activity extract of (activity), its definite ingredients, pharmacologically active is obvious, stable performance, safe and reliable, with this extract is that main component can be processed into the appropriate drug preparation and be used for the treatment of diabetes, the effect of have heat-clearing and toxic substances removing, quenching the thirst proves that through pharmacodynamic experiment it is evident in efficacy.Extract raw material sources of the present invention are easy to get, and preparation technology is easy to industrialization, have driven the abundant development and utilization of minority area herb resource.
The specific embodiment
Following embodiment 1-6 is the preparation example of Radix Musae extract.
Embodiment 1: bright product Radix Musae is squeezed the juice with juice extractor through cutting into slices, smashing to pieces, the juice n-butanol extraction, and 40 ℃～65 ℃ concentrating under reduced pressure, 40 ℃～65 ℃ vacuum decompression dryings promptly get extract X (corresponding extractum C).
Embodiment 2: bright product Radix Musae is through cutting into slices, smashing to pieces, extract 5 times with 50% soak with ethanol (30 ℃～60 ℃) earlier, again residue is squeezed the juice, merge alcohol extract and squeezeding juice, 25 ℃～65 ℃ are evaporated to nothing alcohol flavor, use aqueous dispersion, the reuse n-butanol extraction, 25 ℃～65 ℃ concentrating under reduced pressure, 25 ℃～65 ℃ constant pressure and dries promptly get extract X (corresponding extract F).
Embodiment 3: bright product Radix Musae is through cutting into slices, smashing to pieces, extract 6 times with 75% soak with ethanol (10 ℃～75 ℃) earlier, again residue is squeezed the juice, merge alcohol extract and squeezeding juice, 35 ℃～75 ℃ concentrating under reduced pressure, 35 ℃～75 ℃ constant pressure and dries, use aqueous dispersion, reuse n-butanol extraction, 35 ℃～75 ℃ concentrating under reduced pressure, 35 ℃～75 ℃ spray dryinges promptly get extract X (corresponding extract F).
Embodiment 4: get the n-butanol extraction extractum that obtains in EXAMPLE l or 2 or 3, water is dissolving as far as possible, last macroporous adsorptive resins, use water, 50% ethanol elution of 2～7 times of column volumes respectively, discard water elution liquid, collect ethanol elution, 50 ℃～70 ℃ concentrating under reduced pressure,-25 ℃～-55 ℃ lyophilizations get extract Y (being Y-2).
Embodiment 5: get the n-butanol extraction extractum that obtains in embodiment 1 or 2 or 3, water is dissolving as far as possible, last macroporous adsorptive resins, use water, 30% ethanol elution of 2～7 times of column volumes respectively, discard water elution liquid, collect ethanol elution, 30 ℃～60 ℃ concentrating under reduced pressure, 30 ℃～60 ℃ spray dryinges get extract Y (being Y-1).
Embodiment 6: get the n-butanol extraction extractum that obtains in embodiment 1 or 2 or 3, water is dissolving as far as possible, last macroporous adsorptive resins, use water, 70% ethanol elution of 2～7 times of column volumes respectively, discard water elution liquid, collect ethanol elution, concentrating under reduced pressure, drying gets extract Y (being Y-3).
Following embodiment 7-10 is the application examples of Radix Musae extract, is about to the pharmaceutical preparation that Radix Musae extract is made the treatment diabetes.
Embodiment 7: get above-mentioned Radix Musae extract Y 100mg, the hard capsule of packing into promptly gets capsule.Said preparation day obeys 3 times, each 1.
Embodiment 8: it is an amount of to get above-mentioned Radix Musae extract Y, adds an amount of solubilizing agent, grinds, and adds a small amount of water for injection dilution again, and mixing adds an amount of sodium chloride, adds the injection water after the dissolving again to ormal weight, filter, and embedding, sterilization promptly gets injection.Said preparation is injected 1 time every day.
Embodiment 9: it is an amount of to get above-mentioned Radix Musae extract X and/or Y, adds an amount of solubilizing agent, grinds, and adds the low amounts of water dilution again, and mixing adds correctives and antiseptic, and mixing adds water to ormal weight, filter, and mixing, packing, sterilization promptly gets oral liquid.Said preparation day obeys 3 times, each 20ml.
Embodiment 10: it is an amount of to get above-mentioned Radix Musae extract X and/or Y, adds an amount of conventional adjuvant, and mixing is made granule, drying, and compacting promptly gets tablet in flakes.Said preparation day obeys 3 times, each 1.
Claims (7)
1. the preparation method of Radix Musae extract is characterized in that: may further comprise the steps:
A, with aquatic foods product Radix Musae section, smash to pieces, squeeze the juice with juice extractor, the juice n-butanol extraction, concentrating under reduced pressure, drying must extract X;
B, with steps A gained n-butanol extraction extractum water dissolution, last macroporous adsorptive resins is used water, 30%～70% ethanol elution of 2～7 times of column volumes respectively, discards water elution liquid, collects ethanol elution, concentrating under reduced pressure, drying, extract Y.
2. according to the preparation method of the described Radix Musae extract of claim 1, it is characterized in that: may further comprise the steps:
A, with aquatic foods product Radix Musae section, smash to pieces, extract 4～6 times with 0～95% soak with ethanol earlier, again residue is squeezed the juice merging alcohol extract and squeezeding juice, being evaporated to does not have the alcohol flavor, directly with aqueous dispersion or dry back aqueous dispersion, reuse n-butanol extraction, concentrating under reduced pressure, drying gets extract X;
B, with steps A gained n-butanol extraction extractum water dissolution, last macroporous adsorptive resins is used water, 50% ethanol elution of 2～7 times of column volumes respectively, discards water elution liquid, collects ethanol elution, concentrating under reduced pressure, drying, extract Y.
3. according to the preparation method of claim 1 or 2 described Radix Musae extracts, it is characterized in that: concentrating under reduced pressure described in steps A and the step B carries out under 25 ℃～75 ℃ conditions, and described drying is carried out under 25 ℃～75 ℃ or-25 ℃～-55 ℃ conditions.
4. according to the preparation method of claim 1 or 2 described Radix Musae extracts, it is characterized in that: dry vacuum decompression drying, constant pressure and dry, lyophilization or the spray drying of adopting described in steps A and the step B.
5. according to the preparation method of the described Radix Musae extract of claim 2, it is characterized in that: soak with ethanol extraction temperature is 10 ℃～75 ℃ in the steps A.
6. as the preparation method of Radix Musae extract prepares as described in each among the claim 1-5 extract X or extract Y.
7. Radix Musae extract X as claimed in claim 6 or the extract Y application in the medicine of preparation treatment diabetes.
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| CN109942385A (en) * | 2019-04-19 | 2019-06-28 | 贵州医科大学 | Three noval chemical compounds and extraction separation method in root of Japanese banana |
| CN112353881A (en) * | 2020-10-16 | 2021-02-12 | 广西民族大学 | Method for extracting polyphenol substances from banana leaf stalks |
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| 《时珍国医国药》 20101231 钱海兵等 芭蕉根不同提取物的抗炎镇痛作用研究 , 第04期 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103454374A (en) * | 2013-08-29 | 2013-12-18 | 贵州维康药业有限公司 | Quality control method of bone rehabilitation medicine |
| CN109942385A (en) * | 2019-04-19 | 2019-06-28 | 贵州医科大学 | Three noval chemical compounds and extraction separation method in root of Japanese banana |
| CN109942385B (en) * | 2019-04-19 | 2022-06-28 | 贵州医科大学 | Three new compounds in Japanese banana root and extraction and separation method |
| CN112353881A (en) * | 2020-10-16 | 2021-02-12 | 广西民族大学 | Method for extracting polyphenol substances from banana leaf stalks |
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Application publication date: 20111102 |