CN102220295B - Method for producing glucose oxidase for feed by using rice processing by-products as raw materials - Google Patents
Method for producing glucose oxidase for feed by using rice processing by-products as raw materials Download PDFInfo
- Publication number
- CN102220295B CN102220295B CN 201110138571 CN201110138571A CN102220295B CN 102220295 B CN102220295 B CN 102220295B CN 201110138571 CN201110138571 CN 201110138571 CN 201110138571 A CN201110138571 A CN 201110138571A CN 102220295 B CN102220295 B CN 102220295B
- Authority
- CN
- China
- Prior art keywords
- substratum
- glucose oxidase
- seed culture
- inoculation
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000019420 glucose oxidase Nutrition 0.000 title claims abstract description 22
- 108010015776 Glucose oxidase Proteins 0.000 title claims abstract description 20
- 239000004366 Glucose oxidase Substances 0.000 title claims abstract description 20
- 229940116332 glucose oxidase Drugs 0.000 title claims abstract description 20
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 16
- 235000009566 rice Nutrition 0.000 title claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- 240000007594 Oryza sativa Species 0.000 title claims 3
- 239000002994 raw material Substances 0.000 title abstract description 9
- 239000006227 byproduct Substances 0.000 title abstract description 6
- 238000012545 processing Methods 0.000 title abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 28
- 230000004151 fermentation Effects 0.000 claims abstract description 28
- 238000011218 seed culture Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 238000011081 inoculation Methods 0.000 claims abstract description 17
- 239000007787 solid Substances 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 15
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 238000003892 spreading Methods 0.000 claims abstract description 5
- 229920001817 Agar Polymers 0.000 claims description 12
- 244000061456 Solanum tuberosum Species 0.000 claims description 12
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 12
- 239000008272 agar Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 6
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 6
- 239000011425 bamboo Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 235000000346 sugar Nutrition 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 4
- 239000012531 culture fluid Substances 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 230000004763 spore germination Effects 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 238000011177 media preparation Methods 0.000 claims 1
- 241000209094 Oryza Species 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 239000000047 product Substances 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 4
- 241000228150 Penicillium chrysogenum Species 0.000 abstract 3
- 238000010411 cooking Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 239000000243 solution Substances 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 238000013494 PH determination Methods 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The method for producing the glucose oxidase for the feed by taking the rice processing by-products as raw materials comprises the following steps: inoculating penicillium notatum with high yield of glucose oxidase into a 10cm microbial culture dish filled with a PDA culture medium for culture; seed culture: after the liquid seed culture medium is sterilized at 115-121 ℃ for 20-30 min, transferring the activated penicillium notatum in the culture dish to the liquid seed culture medium; preparing a solid fermentation culture medium: mixing the crushed rice and the rice bran according to the weight ratio of 10: 1-3, and cooking for 25-40 min at 90-100 ℃; fourth, inoculation and cultivation: after spreading and cooling the solid fermentation medium, inoculating penicillium notatum seeds for culturing; after the solid fermentation is finished, placing the culture medium at 100-120 ℃ for drying for 2-4 h, and crushing to 40-80 meshes. The invention has wide raw material source, no three wastes, simple process and high activity of the product glucose oxidase up to 200U.
Description
Technical field
The present invention relates to a kind of production method of feeding glucose oxidase, relate in particular to and a kind ofly utilize that paddy processing byproduct joint is cracked rice, rice bran, the method for producing feeding glucose oxidase by solid fermentation.
Background technology
At present, the liquid submerged fermentation method is adopted in the production of feeding glucose oxidase, and there are many deficiencies in the method in concrete the application, for example, the fermentation equipment cost is high, and liquid submerged fermentation needs the fermentation equipment of specialty, such as fermentor tank etc., one 30 tons fermentor tank cost reaches 1,000,000; The fermenting process energy consumption is high, needs constantly to stir; Fermentation staff technical quality is had relatively high expectations; The three wastes (waste water, waste residue, waste gas) environmental pollution heavier grade (JOURNAL OF MICROBIOLOGY 2009,1(29) p86-89; Food and fermentation industries 2003,29 (6) p 87-91).The solution fermentation production of feeding glucose oxidase also has a remarkable shortcoming: fermenting process is raw materials used mainly to be the high-quality caster sugar, raw materials cost high (" feed and herding " 2008(7) p37-39).The developed countries such as the U.S. utilize its scientific and technological advantage, make up the genetic engineering bacterium of high expression level glucose oxidase, the factory construction of liquid fermenting in developing countries such as China, buy again protoenzyme, be mainly used in medicine and food service industry after the separation and purification, it is composite rear for feedstuff industry that part and other enzyme are also arranged, but has equally the higher problem of cost.
Summary of the invention
The objective of the invention is, overcome the present above-mentioned shortcoming that adopts production technology, provide that a kind of raw material sources are wide, equipment cost is low, technique is simple, the production method of the feeding glucose oxidase of environmentally safe.
The objective of the invention is to be achieved through the following technical solutions, a kind of production method of feeding glucose oxidase may further comprise the steps:
⑴ be equipped with the some mould inoculation of high yield glucose oxidase 10 cm microbial culture dishs of potato agar substratum (PDA substratum), cultivates 36 ~ 48 h for 25 ~ 30 ℃;
Described potato agar substratum preparation: peeling potato 200 g liquors 30 min, 4 layers of filtered through gauze are added glucose 20 g in the filtrate, and agar 15 g mend distilled water to 1000 ml, and 115 ℃ of sterilization 20 min pave ware for subsequent use;
⑵ seed culture: liquid seed culture medium is behind 115 ~ 121 ℃ of sterilization 20 ~ 30 min, with the some mould switching liquid seed culture medium that activates in step (1) culture dish, inoculum size is 10 cm culture dish inoculation, 500 ~ 1000 ml liquid seed culture mediums; Cultivate 60 ~ 72 h in 25 ~ 30 ℃ after the inoculation;
Described liquid seed culture medium prescription: 1 ~ 6wt% caster sugar, 0.05 ~ 0.2wt% KH
2PO
4, 0.05 ~ 0.2wt%NaCl, 0.01 ~ 0.06wt%MgSO
47H
2O, 0.1 ~ 0.4wt% NaNO
3: 0.001 ~ 0.025wt% FeCl
3, 0.05 ~ 0.2wt % CaCl
2, Yu Weishui, the pH nature, 115 ℃ of sterilization 20 min are for subsequent use;
⑶ the preparation of solid fermentation substratum: joint cracked rice mix 90 ~ 100 ℃ of boiling 25 ~ 40 min with rice bran by the weight ratio of 10:1 ~ 3;
⑷ inoculation culture: after the spreading for cooling of solid fermentation substratum, the point mould seed that inoculation step (2) obtains, inoculum size is that 500 ~ 1000 ml seed culture fluids are inoculated 500 kg substratum, mix, packing into is covered with in the bamboo basket of gunnysack, covers with gunnysack again, until after the spore germination of some mould, change fermentation bed over to, 25 ~ 30 ℃, cultivate 48 ~ 60 h under the humidity ventilation condition;
⑸ solid fermentation places 100 ~ 120 ℃ of drying 2 ~ 4 h with substratum after finishing, and is crushed to 40 ~ 80 orders.
The present invention adopts that cheap paddy processing byproduct joint is cracked rice, rice bran is substrate, adopt solid-fermented technique, need not the specific installations such as fermentor tank, extraction waits complicated technology without male offspring, produces without " three wastes ", to pollution-free without environment, technique is simple, and is easy to operate, reduced raw materials cost, equipment cost and the production process cost of glucose oxidase, raw material sources are wide, can realize the higher value application of paddy processing byproduct; Both be fit to the fermentation of rural area individual workship, also be fit to industrialized production.Product glucose oxidase enzyme activity is high, and enzymic activity is up to 200 U; Dry 2 h under 100 ~ 120 ℃ of high temperature, enzyme live loss less than 5%.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
⑴ be equipped with the some mould inoculation of high yield glucose oxidase 10 cm microbial culture dishs of potato agar substratum (PDA substratum), cultivates 40 h for 28 ℃;
Described potato agar substratum preparation: peeling potato 200 g liquors 30 min, 4 layers of filtered through gauze are added glucose 20 g in the filtrate, and agar 15 g mend distilled water to 1000 ml, and 115 ℃ of sterilization 20 min pave ware for subsequent use;
⑵ seed culture: liquid seed culture medium prescription: 5wt % high-quality caster sugar, 0.1 wt% KH2PO4,0.1 wt% NaCl, 0.02wt% MgSO47H2O, 0.2 wt% NaNO3:0.0015wt % FeCl3,0.1 wt% CaCl2, the distilled water preparation, the pH nature), behind 115 ℃ of sterilization 20 min, with the some mould switching liquid seed culture medium that activates in the culture dish, inoculum size is that 10 cm culture dish are inoculated 500 ml liquid seed culture mediums; Be cultured to a large amount of formation spores in 28 ℃ after the inoculation;
⑶ the preparation of solid fermentation substratum: joint cracked rice mix 100 ℃ of boiling 30 min with rice bran in the ratio of 10:1;
⑷ inoculation culture: after the spreading for cooling of solid fermentation substratum, vaccination mould seed, inoculum size is that 500 ml seed culture fluids are inoculated 500 kg substratum, mix, packing into is covered with in the bamboo basket of gunnysack, covers with gunnysack again, when temperature rises to 45 ℃, pour out substratum, stir cooling, reinstall in the former bamboo basket, until absinthe-green mildew appears in media surface, change fermentation bed over to, 28 ℃, cultivate 60 h under the humidity ventilation condition;
⑸ solid fermentation places 115 ℃ of drying 2 h with substratum after finishing.Be crushed to 80 orders.
Adopt following glucose oxidase vigour-testing method that the enzyme activity of each embodiment product is detected:
2.5 ml dianisidine damping fluid (0.1 g dianisidine, be dissolved in 10 ml pH6.0 phosphoric acid buffers, get 0.1 ml dianisidine solution before the experiment, join in the 12 ml damping fluids and use), add 0.3 ml, 18% glucose solution and (take by weighing D-dextrose anhydrous 18g, be dissolved in the 100ml distilled water), add 0.1 ml, 0.03% superoxide enzyme solution (take by weighing horseradish peroxidase 10 mg and be dissolved in 30 ml distilled water), 25 ℃ of insulation 3 min add dilution, and good enzyme liquid is read OD
460Changing value at 1 min.
The activity of the glucose oxidase of 1 unit (1 U) is defined as: at 25 ℃, glucose oxidase enzyme catalysis glucose per minute generates 1 μ mol H under the condition determination of pH 6.0
2O
2The enzyme amount be a unit.
After testing, the enzyme activity of the present embodiment product is 203 U.
Embodiment 2
⑴ be equipped with the some mould inoculation of high yield glucose oxidase 10 cm microbial culture dishs of potato agar substratum (PDA substratum), cultivates 36 h for 30 ℃;
Described potato agar substratum preparation: peeling potato 200 g liquors 30 min, 4 layers of filtered through gauze are added glucose 20 g in the filtrate, and agar 15 g mend distilled water to 1000 ml, and 115 ℃ of sterilization 20 min pave ware for subsequent use;
⑵ seed culture: liquid seed culture medium prescription: 10 wt% high-quality caster sugars, 0.15wt% KH2PO4,0.15wt% NaCl, 0.03 wt% MgSO47H2O, 0.3wt% NaNO3:0.003wt% FeCl3,0.15wt% CaCl
2, Yu Weishui, the pH nature, behind 115 ℃ of sterilization 30 min, with the some mould switching liquid seed culture medium that activates in the culture dish, inoculum size is that 10 cm culture dish are inoculated 1000 ml liquid seed culture mediums; Be cultured to a large amount of formation spores in 30 ℃ after the inoculation;
⑶ the preparation of solid fermentation substratum: joint is cracked rice, rice bran mixes 100 ℃ of boiling 40 min in the ratio of 10:3;
⑷ inoculation culture: after the spreading for cooling of solid fermentation substratum, vaccination mould seed, inoculum size is that 600 ml seed culture fluids are inoculated 500 kg substratum, mix, packing into is covered with in the bamboo basket of gunnysack, covers with gunnysack again, when temperature rises to 45 ℃ of left and right sides, pour out substratum, stir cooling, reinstall in the former bamboo basket, until absinthe-green mildew appears in media surface, change fermentation bed over to, 30 ℃, cultivate 50 h under the humidity ventilation condition;
⑸ solid fermentation places 120 ℃ of drying 3 h with substratum after finishing.Be crushed to 80 orders.
After testing, the enzyme activity of the present embodiment product is 221 U.
The above; it only is preferred embodiment of the present invention; be not that the present invention is done any pro forma restriction; any those skilled in the art; within not breaking away from the technical solution of the present invention scope; utilize the technology contents of above-mentioned announcement to make a little change or be modified to the equivalent embodiment of equivalent variations, all still belong to protection scope of the present invention.
Claims (1)
1. the production method of a feeding glucose oxidase is characterized in that, may further comprise the steps:
⑴ be equipped with the some mould inoculation of high yield glucose oxidase 10 cm microbial culture dishs of potato agar substratum, cultivates 36～48 h for 25～30 ℃;
Described potato agar substratum preparation: peeling potato 200 g liquors 30 min, 4 layers of filtered through gauze are added glucose 20 g in the filtrate, and agar 15 g mend distilled water to 1000 ml, and 115 ℃ of sterilization 20 min pave ware for subsequent use;
⑵ seed culture: liquid seed culture medium is behind 115～121 ℃ of sterilization 20～30 min, with the some mould switching liquid seed culture medium that activates in step (1) culture dish, inoculum size is 10 cm culture dish inoculation, 500～1000 ml liquid seed culture mediums; Cultivate 60～72 h in 25～30 ℃ after the inoculation;
Described liquid seed culture medium preparation: 1～6wt% caster sugar, 0.05～0.2wt% KH
2PO
4, 0.05～0.2wt%NaCl, 0.01～0.06wt%MgSO
47H
2O, 0.1～0.4wt% NaNO
3: 0.001～0.025wt% FeCl
3, 0.05～0.2wt % CaCl
2, Yu Weishui, the pH nature, 115 ℃ of sterilization 20 min are for subsequent use;
⑶ the preparation of solid fermentation substratum: joint cracked rice mix 90～100 ℃ of boiling 25～40 min with rice bran by the weight ratio of 10:1～3;
⑷ inoculation culture: after the spreading for cooling of solid fermentation substratum, the point mould seed that inoculation step (2) obtains, inoculum size is that 500～1000 ml seed culture fluids are inoculated 500 kg substratum, mix, packing into is covered with in the bamboo basket of gunnysack, covers with gunnysack again, until after the spore germination of some mould, change fermentation bed over to, 25～30 ℃, cultivate 48～60 h under the humidity ventilation condition;
⑸ solid fermentation places 100～120 ℃ of drying 2～4 h with substratum after finishing, and is crushed to 40～80 orders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110138571 CN102220295B (en) | 2011-05-26 | 2011-05-26 | Method for producing glucose oxidase for feed by using rice processing by-products as raw materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110138571 CN102220295B (en) | 2011-05-26 | 2011-05-26 | Method for producing glucose oxidase for feed by using rice processing by-products as raw materials |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102220295A CN102220295A (en) | 2011-10-19 |
| CN102220295B true CN102220295B (en) | 2013-02-06 |
Family
ID=44777000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110138571 Expired - Fee Related CN102220295B (en) | 2011-05-26 | 2011-05-26 | Method for producing glucose oxidase for feed by using rice processing by-products as raw materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102220295B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381618A (en) * | 2014-12-05 | 2015-03-04 | 长沙理工大学 | Production method of glucose oxidase for feeding |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1845989A (en) * | 2003-09-11 | 2006-10-11 | 诺和酶股份有限公司 | Recombinant production of antimicrobial agents |
-
2011
- 2011-05-26 CN CN 201110138571 patent/CN102220295B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1845989A (en) * | 2003-09-11 | 2006-10-11 | 诺和酶股份有限公司 | Recombinant production of antimicrobial agents |
Non-Patent Citations (2)
| Title |
|---|
| 吴粹文 等.贮存温度和时间对蜂王浆中葡萄糖氧化酶（GOD）活性影响的研究.《中国养蜂》.1990,(第3期),4-6. * |
| 葡萄糖氧化酶协作组.点青霉(Penicillium notatum)AS3.3871葡萄糖氧化酶的研究.《微生物学通报》.1974,(第1期),1-5. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102220295A (en) | 2011-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dung et al. | Functionality of selected strains of moulds and yeasts from Vietnamese rice wine starters | |
| Gungormusler et al. | Continuous production of 1, 3-propanediol using raw glycerol with immobilized Clostridium beijerinckii NRRL B-593 in comparison to suspended culture | |
| CN102796673B (en) | Feruloyl esterase production strain and method for producing feruloyl esterase by using same | |
| Lin et al. | Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol | |
| Germec et al. | Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system | |
| CN103911295B (en) | Trametes trogii bacterial strain and the application in utilizing municipal administration dewatered sludge production laccase thereof | |
| CN104787899B (en) | A kind of marine aquaculture antibacterial type water quality cleansing agent and preparation method thereof | |
| CN102586216A (en) | Method for producing sodium alginate lyase by utilizing vibrio vulnificus | |
| CN102660519A (en) | Method for preparing biological enzyme by utilizing fermentation waste liquid | |
| Jiang et al. | Efficient production of polymer-grade L-lactate by an alkaliphilic Exiguobacterium sp. strain under nonsterile open fermentation conditions | |
| CN103184160A (en) | Strain for producing organic solvent tolerant lipase, organic solvent tolerant lipase and preparation method of organic solvent tolerant lipase | |
| Pimtong et al. | Enhanced effectiveness of Rhizopus oryzae by immobilization in a static bed fermentor for l-lactic acid production | |
| Sebastian et al. | Fumaric acid production using alternate fermentation mode by immobilized Rhizopus oryzae-a greener production strategy | |
| CN101696394A (en) | Method for fermenting high-activity bacillus bacillus and fermented product | |
| CN102220295B (en) | Method for producing glucose oxidase for feed by using rice processing by-products as raw materials | |
| CN103409379B (en) | A kind of Resina Ferulae mushroom and rhodotorula mucilaginosa are total to the method for fermentative production of laccase | |
| CN108220189A (en) | One plant of marine bacteria for producing zytase and application | |
| Sharma et al. | Utilization of agro-industrial residues for pectinase production by the novel strain Pseudozyma sp. SPJ under solid state cultivation | |
| CN102533570A (en) | Aspergillus niger, application of Aspergillus niger and method for preparing citric acid by fermentation | |
| CN101869119A (en) | Biocontrol strain microcapsule microbial agent, preparation method and application thereof | |
| CN115896179A (en) | Method for producing high-added-value product by utilizing brewing wastewater from monascus makinoi extremely | |
| CN103173422A (en) | Solid fermentation medium for producing laccase through utilizing funalia trogii and application thereof | |
| Yang et al. | Study on the solid-state fermentation conditions for producing thermostable xylanase feed in a pressure pulsation bioreactor | |
| CN101892163B (en) | Penicillium camemberti and application thereof | |
| CN104762328A (en) | Fermentation method of increasing anti-bacterial capability of fermentation liquid of marine bacillus subtilis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130206 Termination date: 20150526 |
|
| EXPY | Termination of patent right or utility model |