CN102204577B - Compound ailanthus preparation and applications thereof - Google Patents
Compound ailanthus preparation and applications thereof Download PDFInfo
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- CN102204577B CN102204577B CN 201110082030 CN201110082030A CN102204577B CN 102204577 B CN102204577 B CN 102204577B CN 201110082030 CN201110082030 CN 201110082030 CN 201110082030 A CN201110082030 A CN 201110082030A CN 102204577 B CN102204577 B CN 102204577B
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Abstract
The invention, belonging to the technical field of medical product research and development, relates to a compound ailanthus preparation and applications thereof. The preparation is prepared by the following steps: taking ailanthus leaves, melia azedarach leaves and artemisia capillaris respectively, adding ethanol respectively, extracting under oscillation by using a shaker, centrifugating to obtain the supernatant by a centrifuge; repeating the extraction twice, mixing the supernatants obtained by three times of extraction, filtering the mixture, concentrating the filtrate by reducing pressure, drying the concentrated filtrate to obtain extracts of ailanthus leaves, melia azedarach leaves and artemisia capillaries; adding mashed garlic into a round-bottom flask, carrying out vapour distillation to obtain garlic essential oil; and taking the extracts of ailanthus leaves, melia azedarach leaves, and artemisia capillaries and garlic essential oil to prepare the compound ailanthus preparation. The preparation has bacteriostasis to fusarium moniliforme and botrytis cinerea, can be used for developing corresponding preparations for controlling plant diseases, and is suitable for industrial production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of compound tree-of-heaven preparation and uses thereof.Product of the present invention has bacteriostasis to fusarium moniliforme, botrytis cinerea, can develop corresponding preparations and be used for controlling plant diseases, and product of the present invention is fit to suitability for industrialized production.
Background technology
It is that agricultural development mainly hinders that the caused crop production reduction of pathogenic fungus diseases is received less, people for the control of this difficult problem for many years mainly take chemical control as main, yet because the material that the most right and wrong of chemical pesticide exist naturally, and normal being difficult for degrades, residual life, is longer, natural, ecological is had destructive effects, forms public hazards, some in addition jeopardize human health.Therefore, exploitation efficient to pest, to non-target organism safety, easily decompose and catabolite has become the task of top priority to the harmless botanical pesticide of environment.The exploitation of botanical pesticide has obtained great successes under this opportunity promotes.
Why the exploitation of botanical pesticide can mainly be that to come from its development cost low yielding unusually brilliant results in the recent period, aboundresources, the easily characteristics of degraded, low-residual.Yet in recent years the exploitation of botanical pesticide but with its advantage run in the opposite direction, in order to pursue high activity, some high poison, expensive plant also are used to development and use gradually such as the root of Beijing euphorbia, sweet-scented oleander, the red sage root etc., this has greatly hindered the market expansion of botanical pesticide, the penetration and promotion of retardance botanical pesticide.
The tree-of-heaven leaf is the dried leaves of quassia tree-of-heaven Ailanthus altissima.Tree-of-heaven has " mild toxicity ", and only for frying in shallow oil the soup washout, the tree-of-heaven leaf can not eat.
Folium Meliae is the dried leaves of Meliaceae deciduous tree plant China tree Melia azedarach Linn..Folium Meliae has heat-clearing and damp-drying drug, killing parasites to relieve itshing, promoting qi circulation and relieving pain effect.
Artemisia capillaris is for belonging to the cauline leaf of feverfew Artemisia capillaris Artemisia capillaries.The Artemisia capillaris decoction has obvious inhibitory action to staphylococcus aureus.Shigella dysenteriae, hemolytic streptococcus, Diplococcus pneumopniae, diphtheria bacillus, ox type and Bacillus tuberculosis, Escherichia coli, typhoid bacillus, Pseudomonas aeruginosa, hay bacillus, diplococcus meningitidis etc. there is in various degree inhibitory action.Artemisia capillaris volatile oil has to a certain degree inhibitory action to skin disease pathogenic fungis such as trichophyton gypseum, acrothesium floccosum and microsporon audouinis in vitro, and its active ingredient is capillin.The external application of Artemisia capillaris volatilization ointment can be cured experimental rat skin acrothesium floccosum disease fully.Artemisia capillaris ethanol extract infected by influenza has inhibitory action.
Galic essential oil is the volatile oil that the bulb of garlic Allium sativum L. (Garlic) obtains through steam distillation.Garlic contains that volatile oil is about 0.2%, and main component is allicin in the oil, has bactericidal action, is that alliin contained in the garlic is produced by the effect hydrolysis of large allinase.
Fusarium moniliforme Fusarium moniliforme Sheld. is non-obligate, the specific pathogen of nonhost, mainly pollutes corn, Chinese sorghum, wheat, cotton, tomato, peanut, banana, soybean, green pepper and some feed.The water soluble metabolites fumonisins of fusarium moniliforme (Fumonisin FB) can cause the animal various diseases, such as horse white matter of brain malacosis, pig pulmonary edema or rat liver cancer, shows on evidence that also fumonisins is relevant with the rate occurred frequently of human esophagus cancer.
Botrytis cinerea Botrytis cinerea is the botrytis in the fungi, and characteristics are that saprophytic ability a little less than the parasitic ability, is facultative parasite by force, host range is wide, can encroach on the vegetables of many types, gray mold nearly all can occur in common vegetables, so be difficult to prevent and treat with changing kind such as the change of crops.Iprodione, procymidone of preventing and treating in the past thiophanate methyl, the dicarboximide class of carbendazim, the thiocarbamates of the bactericide of gray mold such as benzimidazole the eighties in 20 century etc. used nearly 30 years, and ash arrhizus bacteria has all produced pesticide resistance in various degree to these medicaments.
Summary of the invention
1, a kind of compound tree-of-heaven preparation it is characterized in that the preparation of being made by the following weight proportioning:
Tree-of-heaven leaf extract 2g-3g, Folium Meliae extract 1g-2g, Artemisia capillaris extract 1g-2g, galic essential oil 0.001g-0.002g.
2, the preparation method of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that may further comprise the steps:
Step (one): get respectively each 30g-40g of tree-of-heaven leaf, Folium Meliae and Artemisia capillaris, add respectively 600mL-800mL ethanol, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added respectively 400mL-600mL ethanol, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added respectively 200mL-400mL ethanol, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, and merges three times supernatant, filters, get filtrate, filtrate is carried out reduced pressure concentration, and oven dry obtains tree-of-heaven leaf, Folium Meliae and Artemisia capillaris extract;
Step (four): get the garlic 10g-20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h-2.0h leaves standstill, and gets galic essential oil;
Step (five): make compound tree-of-heaven preparation by weight proportion claimed in claim 1.
3, the purposes of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that fusarium moniliforme, botrytis cinerea are had bacteriostasis.
According to the present invention, " % " among the present invention is percentage by weight.
Embodiment
A kind of compound tree-of-heaven preparation and uses thereof that relates to of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
1, a kind of compound tree-of-heaven preparation it is characterized in that the preparation of being made by the following weight proportioning:
Tree-of-heaven leaf extract 2g, Folium Meliae extract 1g, Artemisia capillaris extract 1g, galic essential oil 0.001g.
2, the preparation method of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that may further comprise the steps:
Step (one): get respectively each 30gg of tree-of-heaven leaf, Folium Meliae and Artemisia capillaris, add respectively 600mL ethanol, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added respectively 400mL ethanol, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added respectively 200mL ethanol, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, and merges three times supernatant, filters, get filtrate, filtrate is carried out reduced pressure concentration, and oven dry obtains tree-of-heaven leaf, Folium Meliae and Artemisia capillaris extract;
Step (four): get the garlic 10g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h leaves standstill, and gets galic essential oil;
Step (five): make compound tree-of-heaven preparation by weight proportion claimed in claim 1.
3, the purposes of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that fusarium moniliforme, botrytis cinerea are had bacteriostasis.
Embodiment 2:
1, a kind of compound tree-of-heaven preparation it is characterized in that the preparation of being made by the following weight proportioning:
Tree-of-heaven leaf extract 3g, Folium Meliae extract 2g, Artemisia capillaris extract 2g, galic essential oil 0.002g.
2, the preparation method of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that may further comprise the steps:
Step (one): get respectively each 40g of tree-of-heaven leaf, Folium Meliae and Artemisia capillaris, add respectively 800mL ethanol, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added respectively 600mL ethanol, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added respectively 400mL ethanol, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, and merges three times supernatant, filters, get filtrate, filtrate is carried out reduced pressure concentration, and oven dry obtains tree-of-heaven leaf, Folium Meliae and Artemisia capillaris extract;
Step (four): get the garlic 20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 2.0h leaves standstill, and gets galic essential oil;
Step (five): make compound tree-of-heaven preparation by weight proportion claimed in claim 1.
3, the purposes of a kind of compound tree-of-heaven preparation claimed in claim 1 is characterized in that fusarium moniliforme, botrytis cinerea are had bacteriostasis.
Pharmacological evaluation
The bacteriostasis experiment of compound tree-of-heaven preparation
1 material and method
1.1 for the examination material
1.1.1 strains tested
Table 1 disease fungus
Germ is cultivated with potato agar solid culture medium (PDA) in the test.
1.1.2 test apparatus
Superclean bench, illumination box, electronic cursor slide calliper rule, centrifuge, Rotary Evaporators, baking oven, medicinal herb grinder, high-pressure sterilizing pot etc.
1.1.3 test plant sample
It is tree-of-heaven leaf, Folium Meliae, Artemisia capillaris, garlic that plant is chosen in this experiment.
1.2 experimental technique
1.2.1 the preparation of plant extracts
Get respectively each 40g of tree-of-heaven leaf, Folium Meliae and Artemisia capillaris, add respectively 800mL ethanol, with shaking table concussion lixiviate 24h, with centrifugal supernatant (1) and the residue (1) of getting of centrifuge; Residue (1) is added respectively 600mL ethanol, with shaking table concussion lixiviate 18h, centrifugal supernatant (2) and the residue (2) of getting of centrifuge; Residue (2) is added respectively 400mL ethanol, concussion lixiviate 12h, centrifugal supernatant (3) and the residue (3) of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out reduced pressure concentration, and oven dry obtains tree-of-heaven leaf, Folium Meliae and Artemisia capillaris extract; Get the garlic 20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 2.0h leaves standstill, and gets galic essential oil;
Get tree-of-heaven leaf extract 3g, Folium Meliae extract 2g, Artemisia capillaris extract 2g, galic essential oil 0.002g make compound tree-of-heaven preparation.
1.2.2 compound tree-of-heaven preparation is to the mensuration of plant pathogenic fungi bacteriostatic activity
Adopt growth rate method to embody plant extracts to the impact of pathogen mycelial growth rate.Malicious medium is with in preparation: get proofread and correct after the 100ml conical flask, add 20mL PDA, rear adding 5g compound tree-of-heaven preparation adds the PDA constant volume to 100mL again and shakes up, compound tree-of-heaven formulation concentrations is 50mg/mL.Solvent control adds the equivalent solvent, and it is 500 μ g/mL that positive control adopts carbendazim to make medium concentration.To be with malicious medium and contrast to place 25 ℃ of incubators to cultivate, measure with vernier calipers right-angled intersection method and the record Hyphal length every day.Calculate bacteriostasis rate according to following formula, charge to table 1.
Colony diameter=actual measurement colony diameter-6mm (bacterium cake diameter)
Relative inhibition=(solvent control colony diameter-band toadstool fall diameter)/solvent control colony diameter * 100%
2 results
Compound tree-of-heaven preparation sees Table 2 to the mycelial growth rate inhibitory action of disease fungus.
Table 2 compound tree-of-heaven preparation is to the mycelial growth rate inhibitory action of disease fungus
The bacteriostasis rate that calculates is the 5th day entry data in the table.
Claims (2)
1. the preparation method of a compound tree-of-heaven preparation is characterized in that may further comprise the steps:
Step (one): weight proportion is tree-of-heaven leaf extract 2g-3g, Folium Meliae extract 1g-2g, Artemisia capillaris extract 1g-2g, galic essential oil 0.001g-0.002g;
Step (two): get respectively each 30g-40g of tree-of-heaven leaf, Folium Meliae and Artemisia capillaris, add respectively 600mL-800mL ethanol, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added respectively 400mL-600mL ethanol, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (four): residue in (three) is added respectively 200mL-400mL ethanol, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, and merges three times supernatant, filters, get filtrate, filtrate is carried out reduced pressure concentration, and oven dry obtains tree-of-heaven leaf, Folium Meliae and Artemisia capillaris extract;
Step (five): get the garlic 10g-20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h-2.0h leaves standstill, and gets galic essential oil;
Step (six): make compound tree-of-heaven preparation by step () weight proportion.
2. the purposes of a kind of compound tree-of-heaven preparation of method preparation as claimed in claim 1 is characterized in that for the preparation of the pharmaceutical preparation that fusarium moniliforme, botrytis cinerea is had bacteriostasis.
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| CN 201110082030 CN102204577B (en) | 2011-03-28 | 2011-03-28 | Compound ailanthus preparation and applications thereof |
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| CN 201110082030 CN102204577B (en) | 2011-03-28 | 2011-03-28 | Compound ailanthus preparation and applications thereof |
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| CN102204577A CN102204577A (en) | 2011-10-05 |
| CN102204577B true CN102204577B (en) | 2013-05-29 |
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| CN115251091B (en) * | 2022-08-05 | 2023-01-31 | 山东大学 | Biological control method for inhibiting growth of exotic plant torch tree |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1402992A (en) * | 2001-09-06 | 2003-03-19 | 丁小武 | Disease-preventing plant vermifuge and its prepn |
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
| CN101233865A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide special for killing aphids and preparation thereof |
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| JP4065005B1 (en) * | 2006-12-27 | 2008-03-19 | 柳 智玲 | Plant pest control composition |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1402992A (en) * | 2001-09-06 | 2003-03-19 | 丁小武 | Disease-preventing plant vermifuge and its prepn |
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
| CN101233865A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide special for killing aphids and preparation thereof |
Non-Patent Citations (3)
| Title |
|---|
| JP特开2008-162920A 2008.07.17 |
| 广西杀虫植物资源;陈新华等;《广西园艺》;20051231;第16卷(第6期);第58-61页第"附表广西杀虫植物名录"部分 * |
| 陈新华等.广西杀虫植物资源.《广西园艺》.2005,第16卷(第6期),第58-61页第"附表广西杀虫植物名录"部分. |
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