CN102178027B - Method for separating and purifying abalone proteins - Google Patents
Method for separating and purifying abalone proteins Download PDFInfo
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- CN102178027B CN102178027B CN201110123172A CN201110123172A CN102178027B CN 102178027 B CN102178027 B CN 102178027B CN 201110123172 A CN201110123172 A CN 201110123172A CN 201110123172 A CN201110123172 A CN 201110123172A CN 102178027 B CN102178027 B CN 102178027B
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Abstract
The invention relates to a method for separating and purifying abalone proteins, which comprises the following steps of: 1, pulping washed abalone visceral organs, processing the obtained pulp in a high-voltage pulse electric field, centrifuging and removing supernatant fluid containing polysaccharide and fat to obtain abalone protein precipitates; and 2, washing proteins separated from the abalone visceral organs in edible alcohol, centrifuging, taking out the precipitates and eluting the precipitates repeatedly. Due to the adoption of the combination of a PEF (high pressure polyethylene) cell membrane electroporation technology and an ethanol decoloration technology, the yield and the purity of the proteins can be effectively improved and the obtained proteins have invariance property and high quality. The proteins can be further hydrolyzed to prepare condiments or functional health care products.
Description
Technical field
The present invention relates to aquatic products processing leftover bits and pieces manufacture field.
Background technology
[0002] abalone is famous and precious precious marine product, and nutritive value is abundant, but stench problem is difficult to solve in the abalone protein production, usually becomes discarded object, is not used and causes very big environmental pollution.
Pair report that abalone albumen carries out separation and purification is not arranged at present both at home and abroad as yet.
Summary of the invention
The present invention provides a kind of method of separation and purification abalone albumen, and this method is intended to improve the abalone added value; For marine fishery prepares flavouring and functional health product provides good raw material; Reduce of pollution and the energy resource consumption of abalone deep-processing process to environment.
Technical scheme of the present invention is: it may further comprise the steps
A) fresh abalone internal organs are cleaned after, add the 1-5 water doubly of abalone organ weights after, making beating obtains abalone internal organs pretreatment fluid, carries out the B step;
B) after under low temperature or the normal temperature pretreatment fluid being handled through high-pressure pulse electric, obtain the PEF treatment fluid, carry out the C step;
C) treatment fluid of above-mentioned steps is centrifugal, remove supernatant, get sediment, carry out the D step;
D) ethanol decolorization takes off raw meat: will be from the abalone internal organs isolated albumen, after the washing, the centrifuging and taking deposition repeats wash-out repeatedly in the edible alcohol of 50%-95%;
E) drying: obtain abalone albumen through super-dry.
Outstanding advantage of the present invention is:
1. the present invention adopts high-pressure pulse electric (PEF) extractive technique to combine ethanol decolorization technology separation and purification abalone albumen first.
2. the present invention's employed processing method in the raw material processing procedure can be removed non-protein ingredient to greatest extent, improves the yield and the purity of albumen.
3. the present invention adopts the problem that high-pressure pulse electric (PEF) extractive technique combines the ethanol decolorization technology to solve the abalone protein decolouring, taken off raw meat.
4. help twice laid, reduce the pollution of refuse environment.
The specific embodiment
Technical scheme of the present invention is by the realization that is described below:
A) raw material is handled: under 1-30 ℃ normal temperature or low temperature, fresh abalone internal organs are rinsed well, removed connective tissue, add distilled water according to feed liquid weight ratio 1:1-1:5, pull an oar, cross the 20-40 mesh sieve, stir, be prepared into abalone internal organs pretreatment fluid.
B) high-pressure pulse electric is handled: under normal temperature or low temperature; Abalone internal organs pretreatment fluid is handled through high-pressure pulse electric; The high-pressure pulse electric of electric-field intensity 15-35kV/cm, frequency 200-3170Hz, pulsewidth 1.5 μ s-17 μ s is handled; Processing time 300-1500 μ s gets the PEF treatment fluid, gets into step C.
C) treatment fluid of above-mentioned steps B is centrifugal, remove supernatant, get sediment, carry out the D step.
Perhaps described C) step is: with the PEF extract centrifugal after, abandon supernatant, obtain albumen precipitation; Under the HAc of volume fraction 3% solution environmental; The chitosan solution of allotment mass fraction 2%-5%; Stir under 50 ℃ of water bath condition, dissolving 10min, treat to be dissolved into fully transparent, thick colloidal solution after, by adding in the supernatant than 1:10 with the PEF extracting liquid volume; And under 30 ℃ of water bath condition, stir 30min, abundant adsorbed proteins; Afterwards, centrifugal, abandon supernatant, obtain albumen precipitation; Merge two step albumen precipitations, get into the D step.
D) ethanol decolorization takes off raw meat
Described D step is: with the albumen precipitation of C step gained add 1-4 doubly this volume the edible alcohol of concentration 50%-95%, the washing back is centrifugal, abandons supernatant; The deposition that obtains is repeated repeatedly by the aforesaid operations step, obtain the abalone albumen of decoloration deodorization.This albumen further hydrolysis prepares flavouring or functional health product.
E) drying
With obtaining the abalone albumen powder after the abalone albumen drying that obtains among the last step D.
Embodiment 1:
Under 10 ℃, get 10kg clean, with fresh abalone internal organs, by solid-liquid ratio 1:1 adding distil water, making beating, cross 40 mesh sieves, stir, be prepared into abalone internal organs pretreatment fluid.Abalone internal organs pretreatment fluid is handled electric-field intensity 15kV/cm, frequency 3170Hz through high-pressure pulse electric; Pulsewidth 1.5 μ s, processing times 1500 μ s gets the PEF treatment fluid; Be to centrifugalize under the condition of 15160G in separation factor, abandon supernatant, obtain abalone albumen precipitation thing.
The edible alcohol washing back that above-mentioned abalone albumen precipitation thing is added 1 times of volume 95% is centrifugal, abandons supernatant.The deposition that obtains is repeated 2 times by above-mentioned alcohol washing step.After the albumen precipitation that obtains redissolved with 1 times of distilled water, the spray-dried abalone albumen powder that obtains.
Embodiment 2:
Under 20 ℃, get the abalone internal organs that 10kg cleaned, went connective tissue, by solid-liquid ratio 1:2 adding distil water, making beating, cross 40 mesh sieves, stir, be prepared into abalone internal organs pretreatment fluid.Abalone internal organs pretreatment fluid is handled through high-pressure pulse electric, electric-field intensity 20kV/cm, frequency 1000Hz, pulsewidth 2.0 μ s, processing times 1200 μ s gets the PEF treatment fluid, abandons supernatant and obtains albumen precipitation; Under the HAc of volume fraction 3% solution environmental; The chitosan solution of allotment mass fraction 3%; Stir under 50 ℃ of water bath condition, dissolving 10min, treat to be dissolved into fully transparent, thick colloidal solution after, by adding than 1:10 with the shitosan extracting liquid volume; And under 30 ℃ of water bath condition, stir 30min, abundant adsorbed proteins.Afterwards, carry out centrifugally, abandon supernatant, obtain albumen precipitation; Merge two step albumen precipitations.
The edible alcohol washing back that albumen precipitation is added 4 times of volumes 65% is centrifugal, abandons supernatant.The deposition that obtains is repeated 2 times by above-mentioned alcohol washing operation step.After the albumen precipitation that obtains redissolved with 2 times of distilled water, evaporative removal residual alcohol wherein obtained the abalone albumen powder again after freeze drying.
Embodiment 3:
Under 30 ℃ of room temperature conditions, the abalone internal organs after getting 10Kg and cleaning go behind the connective tissue to make pretreatment of raw material liquid by after the making beating of solid-liquid ratio 1:4 adding distil water, crossing 20 mesh sieves.Abalone internal organs pretreatment fluid is handled through high-pressure pulse electric, electric-field intensity 25kV/cm, frequency 1500Hz, pulsewidth 5.0 μ s, processing times 800 μ s gets the PEF treatment fluid.With the PEF extract centrifugal after, abandon supernatant and obtain albumen precipitation; The edible alcohol washing back that albumen precipitation is added 3 times of volumes 75% is centrifugal, abandons supernatant.The deposition that obtains is repeated 3 times by the aforesaid operations step, and heated-air drying obtains the abalone albumen powder after crushed.
Embodiment 4:
Under 1 ℃ of room temperature condition, the abalone internal organs after getting 10Kg and cleaning, press the making beating of solid-liquid ratio 1:3 adding distil water, mistake 40 mesh sieves after, make pretreatment of raw material liquid.This pretreatment fluid is handled through high-pressure pulse electric, electric-field intensity 30kV/cm, frequency 500Hz, pulsewidth 8.0 μ s, processing times 500 μ s gets the PEF treatment fluid.Be to centrifugalize under the condition of 15160G in separation factor, abandon supernatant and obtain abalone albumen precipitation thing.
The edible alcohol washing back that above-mentioned abalone albumen precipitation thing is added 2 times of volumes 85% is centrifugal, abandons supernatant.The deposition that obtains is repeated 2 times by above-mentioned alcohol washing step.After the albumen precipitation that obtains redissolved with 1 times of distilled water, the spray-dried abalone albumen powder that obtains.
Embodiment 5:
Under 10 ℃ of room temperature conditions, the abalone internal organs after getting 10Kg and cleaning go to add the making beating of 50L water, homogenate behind the connective tissue, 20 mesh sieves are crossed in this homogenate after, behind the homogeneous, make pretreatment of raw material liquid.Abalone internal organs pretreatment fluid is handled electric-field intensity 35kV/cm, frequency 200Hz, pulsewidth 17.0 μ s, processing times 300 μ s through high-pressure pulse electric.With the PEF extract centrifugal after, abandon supernatant and obtain albumen precipitation; The edible alcohol washing back that albumen precipitation is added 4 times of volumes 50% is centrifugal, abandons supernatant.The deposition that obtains is repeated 3 times by the aforesaid operations step, and heated-air drying obtains the abalone albumen powder after crushed.
Claims (3)
1. the method for a separation and purification abalone albumen, it is characterized in that: it may further comprise the steps,
A) fresh abalone internal organs are cleaned after, add the 1-5 water doubly of abalone organ weights after, making beating obtains abalone internal organs pretreatment fluid, carries out the B step;
B) under 1-30 ℃ temperature, abalone internal organs pretreatment fluid is handled through the high-pressure pulse electric of electric-field intensity 15-35kV/cm, frequency 200-3170Hz, pulsewidth 1.5 μ s-17 μ s, processing time 300-1500 μ s obtains the PEF treatment fluid;
C) treatment fluid of above-mentioned steps is centrifugal, remove supernatant, get sediment, carry out the D step;
D) ethanol decolorization takes off raw meat: will be from the abalone internal organs isolated albumen, after the washing, the centrifuging and taking deposition repeats wash-out repeatedly in the edible alcohol of 50%-95%;
E) drying: obtain abalone albumen through super-dry.
2. the method for separation and purification abalone albumen according to claim 1 is characterized in that: described D step is: with the albumen precipitation of C step gained add 1-4 doubly this volume the edible alcohol of concentration 50%-95%, the washing back is centrifugal, abandons supernatant; The deposition that obtains is repeated repeatedly by the aforesaid operations step, obtain the abalone albumen of decoloration deodorization.
3. the method for separation and purification abalone albumen according to claim 1 is characterized in that: described C) step is: with the PEF extract centrifugal after, abandon supernatant, obtain albumen precipitation; Under the HAc of volume fraction 3% solution environmental; The chitosan solution of allotment mass fraction 2%-5%; Stir under 50 ℃ of water bath condition, dissolving 10min, treat to be dissolved into fully transparent, thick colloidal solution after, by with the PEF extracting liquid volume than in 1: 10 adding supernatant; And under 30 ℃ of water bath condition, stir 30min, abundant adsorbed proteins; Afterwards, centrifugal, abandon supernatant, obtain albumen precipitation; Merge two step albumen precipitations.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104513843A (en) * | 2014-12-30 | 2015-04-15 | 集美大学 | Joint preparation method of polysaccharide and protein peptide |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349600B (en) * | 2015-11-12 | 2018-06-08 | 中国海洋大学 | A kind of fishy-removing-method of gadus protein enzymatic hydrolyzate |
| CN106387304B (en) * | 2016-09-12 | 2019-06-04 | 福建农林大学 | A kind of method of high-pressure pulse electric coupling biological enzymolysis preparation abalone internal organ phosphatide |
| CN111887335B (en) * | 2020-08-17 | 2023-05-02 | 合肥工业大学 | Method for making soft sweet by using fresh water swimming bladder |
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| CN101200717A (en) * | 2007-12-20 | 2008-06-18 | 江南大学 | Highly effective method for extracting protein and nucleic acid in beer waste yeast |
| CN101273779A (en) * | 2008-05-12 | 2008-10-01 | 吉林大学 | Method for preparing food products with high osteocalcin |
| CN101455264A (en) * | 2008-11-15 | 2009-06-17 | 山东好当家海洋发展股份有限公司 | Preparation method of sea-ear active peptide |
| CN101912027A (en) * | 2010-09-01 | 2010-12-15 | 福州日兴水产食品有限公司 | Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200717A (en) * | 2007-12-20 | 2008-06-18 | 江南大学 | Highly effective method for extracting protein and nucleic acid in beer waste yeast |
| CN101273779A (en) * | 2008-05-12 | 2008-10-01 | 吉林大学 | Method for preparing food products with high osteocalcin |
| CN101455264A (en) * | 2008-11-15 | 2009-06-17 | 山东好当家海洋发展股份有限公司 | Preparation method of sea-ear active peptide |
| CN101912027A (en) * | 2010-09-01 | 2010-12-15 | 福州日兴水产食品有限公司 | Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104513843A (en) * | 2014-12-30 | 2015-04-15 | 集美大学 | Joint preparation method of polysaccharide and protein peptide |
| CN104513843B (en) * | 2014-12-30 | 2018-01-19 | 集美大学 | A kind of combined preparation process of polysaccharide and protein peptides |
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