CN102165936B - Device for studying on migration of plant parasitic nematodes - Google Patents
Device for studying on migration of plant parasitic nematodes Download PDFInfo
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- CN102165936B CN102165936B CN201010621097XA CN201010621097A CN102165936B CN 102165936 B CN102165936 B CN 102165936B CN 201010621097X A CN201010621097X A CN 201010621097XA CN 201010621097 A CN201010621097 A CN 201010621097A CN 102165936 B CN102165936 B CN 102165936B
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Abstract
The invention belongs to the technical field of agriculture, and discloses a device for studying on migration of plant parasitic nematodes. The device comprises two flowerpots and a plastic tube used for connecting the two flowerpots, wherein holes are arranged in the side walls of the two flowerpots respectively, and two ends of the plastic tube are inserted into the two holes respectively. By arranging a bridge used for migrating parasitic nematodes between the two flowerpots used for growing plants, the influence of different plant root exudates on migration of the plant parasitic nematodes can be accurately reflected, in addition, the device has a simple structure and is easy to operate.
Description
Technical field
The present invention relates to agricultural technology field, particularly relate to a kind of device of studying plant nematode taxis migration in soil.
Background technology
Plant nematode is the causal organism that one type of host is extensive, harm is huge; Serious inhibition the yield and quality of a lot of crops; In recent years; The means of preventing and treating nematode in the world more and more trend towards means non-chemically; Mainly be the harm of controlling pathogenic nematode through the information exchanging process between interference crop root and the pathogenic nematode, and this process need is understood nematode semiochemicals how the perception root system of plant discharges and is carried out the taxis migration and infect the biological mechanism of plant, how through a kind of special device the breakthrough bottleneck that dynamic follow-up study becomes this area research can accurately simulated and carry out to this process.At present, nematode taxis Research on motion only is confined to breadboard simulated experiment, mainly utilizes the medium culture plant, and the chemical substance signal sensing how the research nematode discharges plant is also carried out the taxis migration.In fact; In soil environment; Under the different cultivation systems; Different secretions from plant roots are more complicated to the influence of nematode taxis, and for the influence of different secretions from plant roots to the nematode taxis, breadboard simulated experiment can't reflect more accurately that different plant signal are to the influence of nematode taxis in the soil environment.
Summary of the invention
The technical problem that (one) will solve
The technical problem that the present invention will solve is to overcome the simulated experiment of carrying out in the laboratory can't accurately reflect the limitation of the semiochemicals of different plants in the soil environment to parasitic nematode taxis migration influence.
(2) technical scheme
In order to solve the problems of the technologies described above, the present invention provides a kind of device of studying plant nematode taxis migration in soil, and it comprises: two flowerpots, and the plastic tube that connects said two flowerpots; Offer hole respectively on said two flowerpot sidewalls, insert respectively in said two holes at the two ends of said plastic tube.
In the device of above-mentioned research plant nematode taxis migration in soil, the both ends open of said plastic tube is blocked by plastic wire respectively.
In the device of above-mentioned research plant nematode taxis migration in soil, said plastic tube middle part is offered porose.
In the device of above-mentioned research plant nematode taxis migration in soil, the diameter of described hole is 5cm, and the top edge 5cm of the said flowerpot of distance.
In the device of above-mentioned research plant nematode taxis migration in soil, the diameter of said plastic tube is 5cm, and length is 20cm, and the diameter in the hole on the said plastic tube is 1cm.
In the device of above-mentioned research plant nematode taxis migration in soil, the diameter of mesh is 5mm-10mm on the said plastic wire.
In the device of above-mentioned research plant nematode taxis migration in soil, said plastic wire is fixed on the said plastic tube by rubber band.
(3) beneficial effect
The device of the research plant nematode that technique scheme provided taxis migration in soil; Through between two flowerpots of plantation plant, setting up the bridge that supplies the parasitic nematode migration; Can reflect accurately that different secretions from plant roots are to parasitic nematode taxis migration influence; And simple in structure, operation easily.
Description of drawings
Fig. 1 is the structural representation of research plant nematode device of taxis migration in soil of the embodiment of the invention.
Wherein, 1: flowerpot; 2: plastic tube; 3: hole; 4: plastic wire; 5: the hole; 6: rubber band.
Embodiment
Below in conjunction with accompanying drawing and embodiment, specific embodiments of the invention describes in further detail.Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Fig. 1 shows the structural representation of an embodiment of the present invention; Comprise on the sidewall of 1, two flowerpot 1 of two flowerpots offering a hole 3 respectively, use the two ends of plastic tube 2 to insert respectively in two holes 3; Thereby two flowerpots 1 are coupled together; The both ends open of plastic tube 2 gets up with plastic wire 4 envelopes, and with rubber band 6 plastic wire 4 is fixed on the plastic tube 2, and the middle part of plastic tube 2 also offers a hole 5.
The plant nematode that is present in the soil is a kind of more serious causal organism of plant that endangers; Its body length is generally about 1mm; Growth to two nematode in the length of time carries out the taxis motion through the olfactory nerves unit perception plant signal of head and searches out host plant, thereby relies on host plant to accomplish its life cycle.Present embodiment has utilized this characteristic of nematode exactly; With the two ends of plastic tube 2 respectively with two flowerpots 1 on hole 3 link together, accomplish a special bridging device, wherein flowerpot 1 size is 180cm*160cm; Hole 3 is arranged on apart from flowerpot 1 top edge 5cm place, and the diameter of hole 3 is 5cm; The length of plastic tube 2 is 20cm; Diameter is 5cm, and the diameter in the hole 5 beaten, position is 1cm therebetween, and the mesh diameter of the employed plastic wire 4 in plastic tube 2 two ends is between 5mm-10mm; Preferably use the plastic wire 4 of mesh diameter in the present embodiment as 5mm; The effect of plastic wire 4 is: the root system of plant in the flowerpot 1 retaining outside plastic tube 2, to guarantee that root system can not arrive in the plastic tube 2, is guaranteed that material that root system just discharges exerts an influence to the motion of nematode; Simultaneously, the pore size of plastic wire 4 can guarantee nematode freely-movable in soil again.During test; Add the soil of 1/2 volume in the plastic tube 2, after two ports seal with plastic wire 4 respectively, be inserted in two flowerpots 1 that are equipped with hole 3; Plastic tube 2 plays a bridge two flowerpots 1 of planting plants is coupled together; In both sides flowerpot 1, plant different plants according to test objective, nematode injected by the holes in the middle of the plastic tube 25, inoculate 14 days after; Through quantity and the plant roots footing amount of nematode in the both sides soil in the plastic tube 2, prove of the influence of different secretions from plant roots to the migration of nematode taxis.
Can find out by above embodiment; The embodiment of the invention is through setting up the bridge that supplies the parasitic nematode migration between two flowerpots of plantation plant; Can accurately reflect different secretions from plant roots to parasitic nematode taxis migration influence, and simple in structure, operation easily.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and replacement, these improvement and replacement also should be regarded as protection scope of the present invention.
Claims (4)
1. the device of research plant nematode taxis migration in soil is characterized in that, comprising: two flowerpots, and the plastic tube that connects said two flowerpots; Offer hole respectively on said two flowerpot sidewalls, insert respectively in said two holes at the two ends of said plastic tube;
The both ends open of said plastic tube is blocked by plastic wire respectively; Said plastic tube middle part is offered porose; The diameter of mesh is 5mm-10mm on the said plastic wire.
2. the device of research plant nematode as claimed in claim 1 taxis migration in soil is characterized in that the diameter of described hole is 5cm, and the top edge 5cm of the said flowerpot of distance.
3. the device of research plant nematode as claimed in claim 1 taxis migration in soil is characterized in that the diameter of said plastic tube is 5cm, and length is 20cm, and the diameter in the hole on the said plastic tube is 1cm.
4. the device of research plant nematode as claimed in claim 1 taxis migration in soil is characterized in that said plastic wire is fixed on the said plastic tube by rubber band.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010621097XA CN102165936B (en) | 2010-12-24 | 2010-12-24 | Device for studying on migration of plant parasitic nematodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010621097XA CN102165936B (en) | 2010-12-24 | 2010-12-24 | Device for studying on migration of plant parasitic nematodes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102165936A CN102165936A (en) | 2011-08-31 |
| CN102165936B true CN102165936B (en) | 2012-07-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010621097XA Active CN102165936B (en) | 2010-12-24 | 2010-12-24 | Device for studying on migration of plant parasitic nematodes |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202268B (en) * | 2013-03-25 | 2015-04-22 | 南京林业大学 | Biological assay method of microbial volatile compound for influencing approach behavior of nematodes |
| CN103355258B (en) * | 2013-07-26 | 2014-09-24 | 云南农业大学 | Method and device for determining chemical substance taxis of nematodes |
| CN109937969A (en) * | 2019-02-28 | 2019-06-28 | 山东省果树研究所 | A kind of device and application method detecting entomopathogenic nematode short distance capacity of orientation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766100A (en) * | 2009-12-25 | 2010-07-07 | 山东省农业科学院土壤肥料研究所 | Device for researching plant root exudate allelopathy and application method thereof |
-
2010
- 2010-12-24 CN CN201010621097XA patent/CN102165936B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766100A (en) * | 2009-12-25 | 2010-07-07 | 山东省农业科学院土壤肥料研究所 | Device for researching plant root exudate allelopathy and application method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘青娥等.植物线虫的研究和防治.《安徽农业科学》.2006,第34卷(第18期),4644-4645、4664. * |
| 符美英等.根结线虫与寄主植物互作机理的研究进展.《热带农业科学》.2008,第28卷(第3期),73-77. * |
| 陈志星等.一种新型培养与收集伞滑刃线虫的装置.《云南农业大学学报》.2010,第25卷(第3期),443-446. * |
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| CN102165936A (en) | 2011-08-31 |
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