CN102162140A - Microfluid chip and spinning method thereof - Google Patents
Microfluid chip and spinning method thereof Download PDFInfo
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- CN102162140A CN102162140A CN2011100072307A CN201110007230A CN102162140A CN 102162140 A CN102162140 A CN 102162140A CN 2011100072307 A CN2011100072307 A CN 2011100072307A CN 201110007230 A CN201110007230 A CN 201110007230A CN 102162140 A CN102162140 A CN 102162140A
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- 238000009987 spinning Methods 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000000243 solution Substances 0.000 claims abstract description 52
- 239000000758 substrate Substances 0.000 claims abstract description 38
- 238000000578 dry spinning Methods 0.000 claims abstract description 11
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 8
- 230000005684 electric field Effects 0.000 claims abstract description 7
- 238000002166 wet spinning Methods 0.000 claims abstract description 7
- 229920000642 polymer Polymers 0.000 claims abstract description 3
- 239000012530 fluid Substances 0.000 claims description 79
- 238000004804 winding Methods 0.000 claims description 18
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
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- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
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- 239000004471 Glycine Substances 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
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Abstract
The invention relates to a microfluid chip and a spinning method thereof, particularly to a microfluid chip, width of a microfluid passageway being gradually reduced from an inlet to an outlet, and a spinning method thereof. The microfluid chip of the invention comprises a substrate and a diaphragm, wherein the diaphragm is provided with a groove, a surface of the diaphragm, where the groove is arranged in, is bonded with the substrate to form the microfluid passageway, the depth of the whole pathway is uniform, the width (b) of the microfluid passageway is gradually reduced from the inlet to the outlet. By the spinning method of the microfluid chip provided by the present invention, spinning stock solution is injected into the inlet of the microfluid passageway of the microfluid chip and finally flows out from the outlet of the microfluid passageway. The microfluid chip can perform dry spinning under atmosphere condition, conduct wet spinning in polymer coagulation bath, or conduct electrostatic spinning under high voltage electric field. The chip provided by the invention is simple in preparation and low in cost. Besides, drawing and cutting conditions needed by the solution in the chip can be regulated and controlled according to the requirement of spinning technology, thus, the chip has good application prospect.
Description
Technical field
The invention belongs to microfluid, textile fabric, material science, bionic technical field, relate to a kind of micro-fluid chip and spinning process thereof, specifically relate to micro-fluid chip and spinning process thereof that a kind of microfluidic channel width is reduced gradually continuously by the outlet that enters the mouth.
Background technology
In recent years, the positive paid more and more attention of the application of micro-fluid chip aspect fiberizing.Micro-fluidic technologies can be very suitable for the processing of materials such as large biological molecule in spinning under the normal temperature and pressure conditions.At present, the microfluid spining technology spin immediately, static spins the field and obtained good application.In addition, the The Nomenclature Composition and Structure of Complexes that microfluid spinning system might regulate and control spinning solution by the laminar flow and the diffusion property of microfluid with structure and the function of simulating biological spinner, makes controlled, the well behaved fiber of structure.But, spin the laminar flow characteristics that has mainly utilized microfluid with electrostatic spinning technique immediately, and the spinning solution viscosity is little, required micro-fluid chip is all fairly simple, and is less demanding to pipe diameter.The progress of micro-fluid chip aspect fiberizing also provides a kind of new method for the research of Special Category fiber.Existing research has been reported and has been utilized micro-fluid chip that the component of regenerated silk albumen is regulated.Patent WO 2007/141131A1 and US2010029553 (A1) have designed a micro-fluid chip, utilize the pH value and the concentration of metal ions of the laminar flow characteristics adjusting regenerated silk fibroin of microfluid, are used to prepare the protein product of different shape; People such as Rammensee also on the basis of this patent, have studied the influence of shear stress to reorganization spider traction fiber albumen eADF3 and eADF4 gathering assembling by the rectangular channel of different in width is combined.But the patented invention and the bibliographical information that also the form parameter of biological spinning system are not used at present spinning.
Such as the bionical spinning of regenerated silk fibroin, silkworm and spider utilize the interior water of body as the solvent dry spinning at normal temperatures and pressures, the energy-conservation and environmental protection of whole process, and whole spinning process is low energy consumption, high efficiency model.Studies show that the natural animal silk-fibroin is one in organism spinning system flow process and integrates the process of making overall plans and coordinate that pH reduces, metal ion content changes and fibroin concentration raises.In this course, the silk-fibroin that is shaped as of spider and the gradual change of silkworm body spinning pipeline provides the shear tension effect, impels random coil and/or the helical conformation of animal silk-fibroin conformation by solubility, becomes water-fast β-sheet conformation.Bionical spinning to regenerated silk fibroin all is pH value, calcium ion and the fibroin concentration of static adjustment spinning solution after be drawn into silk at present, does not all relate to the shear tension system in the spinning process.Though spider silk has superpower intensity and elasticity, because spider silk can't obtain in a large number.And silk also is a kind of natural fabric of function admirable, and the spinning process of silkworm and spider is closely similar, and the silk wide material sources, and the content of alanine and glycine was the highest during the amino acid of fibroin albumen and spider silk fibroin was formed.Therefore, how to utilize fibroin to become the problem that scientist studies for the regenerated fiber that feedstock production has excellent mechanical property.This has very important significance in macromolecular material and bionical field.
The present invention from simulation spider or silkworm spinning process be starting point, the design microfluidic channel adopts the higher microfluid dry-spinning process of bionical degree, with the high concentration spinning solution, through shear tension, obtains the fiber of function admirable.
Summary of the invention
The object of the present invention is to provide a kind of micro-fluid chip and spinning process thereof, specifically relate to micro-fluid chip and spinning process thereof that a kind of microfluidic channel width is reduced gradually continuously by the outlet that enters the mouth.
A kind of micro-fluid chip of the present invention, comprise substrate and diaphragm, fluted on the diaphragm, reeded of diaphragm and substrate are fitted and are formed microfluidic channel, the degree of depth c of described microfluidic channel whole process equates that described microfluidic channel width b reduces to outlet gradually continuously by about entering the mouth.
As optimized technical scheme:
Aforesaid a kind of micro-fluid chip, the two sides of described microfluidic channel are about microfluidic channel central axis symmetry; Described microfluidic channel, the trace that its two sides and horizontal plane intersect are a kind of or its combination in exponential function curve, hyperbolic function curve or the straight line.
Aforesaid a kind of micro-fluid chip, described exponential function are the second order exponential function.
Aforesaid a kind of micro-fluid chip, described degree of depth c are 10 μ m～500 μ m; Described microfluidic channel, throat width are 500 μ m～2mm, and exit width is 10 μ m～500 μ m.
The present invention also provides a kind of spinning process of micro-fluid chip, spinning solution is injected the inlet of the microfluidic channel of micro-fluid chip, flow out by the outlet of microfluidic channel at last, in air, carry out dry-spinning, or in the polymer coagulating bath, carry out wet spinning, or under the high voltage electric field condition, carry out electrostatic spinning; The degree of depth c of described microfluidic channel whole process equates that described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth.
The spinning process of aforesaid a kind of micro-fluid chip, described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, reeded of diaphragm and substrate are fitted and are formed microfluidic channel; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
The spinning process of aforesaid a kind of micro-fluid chip, described microfluidic channel, the trace that its two sides and horizontal plane intersect is a kind of or its combination in exponential function curve, hyperbolic function curve or the straight line.
The spinning process of aforesaid a kind of micro-fluid chip, described exponential function are the second order exponential function.
The spinning process of aforesaid a kind of micro-fluid chip, described degree of depth c are 10 μ m～500 μ m; Described microfluidic channel, throat width are 500 μ m～2mm, and exit width is 10 μ m～500 μ m.
The spinning process of aforesaid a kind of micro-fluid chip, the injection rate that described spinning solution injects microfluidic channel is 0.5～10 μ L/min; When carrying out dry-spinning, the air-gap from the spinning nozzle to the winding up roller is 1～30cm, and winding speed is 1～5cm/s.
The preparation method of the micro-fluid chip that the present invention proposes, be that exponential function with the body of gland that is similar to spider spider or silkworm and spinner shape function is for designing a model, with the slide is substrate, the SU-8 photoresist is the formpiston material, dimethyl siloxane (PDMS) is the microchannel construction material, is forming technique with photoetching and molding etc.(75 * 25mm) is substrate, and the SU-8 photoresist coating is carried out uv-exposure being printed under the mask of microchannel pattern with slide.Just can obtain the formpiston of microchannel pattern after developing,, solidify the PDMS diaphragm that the back just can obtain being printed on microchannel pattern then at this mould top casting PDMS.At last, the Direct Bonding after Cement Composite Treated by Plasma with this PDMS diaphragm and slide.
The high concentration spinning solution is injected chip in 10～30 ℃ temperature range, directly become silk after extruding by spinning nozzle in air set.The injection rate that described spinning solution injects microfluidic channel is 0.5～10 μ L/min, and the air-gap from the spinning nozzle to the winding up roller is 1～30cm, and winding speed is 1～5cm/s.
Beneficial effect:
(1) prepared microfluidic channel has been simulated the body of gland of spider or silkworm and the shape facility of spinner, has further promoted the bionical degree of spinning process, can be better must be used for the solvent spinning method;
(2) this passage improves spinning effect for the high concentration spinning solution provides in various degree shearing and stretching.
Description of drawings
Fig. 1 is the microfluidic channel schematic diagram
Fig. 2 is the A-A schematic cross-section of Fig. 1
Wherein 1 is that microfluidic channel inlet 2 is microfluidic channel outlets
The specific embodiment
Below in conjunction with the specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
A kind of micro-fluid chip of the present invention, comprise substrate and diaphragm, fluted on the diaphragm, reeded of diaphragm and substrate are fitted and are formed microfluidic channel, the degree of depth c of described microfluidic channel whole process equates, described microfluidic channel width b is reduced to outlet 2 gradually continuously by inlet 1, as illustrated in fig. 1 and 2.
Aforesaid a kind of micro-fluid chip, the two sides of described microfluidic channel are about microfluidic channel central axis symmetry; Described microfluidic channel, the trace that its two sides and horizontal plane intersect are a kind of or its combination in exponential function curve, hyperbolic function curve or the straight line.
Aforesaid a kind of micro-fluid chip, described exponential function are the second order exponential function.
Aforesaid a kind of micro-fluid chip, described degree of depth c are 10 μ m～500 μ m; Described microfluidic channel, throat width are 500 μ m～2mm, and exit width is 10 μ m～500 μ m.
Embodiment 1
With mass percent concentration is that 30% regenerated silk fibroin water solution is a spinning solution, under 30 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 0.5 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt dry-spinning technology, become the silk and the top roll of reeling in air set; Air-gap from the spinning nozzle to the winding up roller is 1cm, and winding speed is 1cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 10 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 2mm, and exit width is 10 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of second order exponential function curve and straight line; Described exponential function formula is: R (x)=ae
Bx+ ce
DxA=-0.004886 wherein, b=0.0003718, c=53.52, d=-9.989*10
-5The length ratio of described curve and straight line is 2: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
With mass percent concentration is that 60% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 10 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt dry-spinning technology, become the silk and the top roll of reeling in air set; Air-gap from the spinning nozzle to the winding up roller is 5cm, and winding speed is 5cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 500 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 1mm, and exit width is 500 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of hyperbolic function curve and straight line; Described hyperbolic function formula is: R (x)=1/2 (e
x-e
-x); The length ratio of described curve and straight line is 1: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 3
With mass percent concentration is that 45% regenerated silk fibroin water solution is a spinning solution, under 10 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 5 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt dry-spinning technology, become the silk and the top roll of reeling in air set; Air-gap from the spinning nozzle to the winding up roller is 10cm, and winding speed is 3cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 500 μ m, and exit width is 250 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is a single order exponential function curve; Described exponential function formula is: R (x)=e
A*ln (x)A=0.0125 wherein; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 4
With mass percent concentration is that 55% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 8 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt dry-spinning technology, become the silk and the top roll of reeling in air set; Air-gap from the spinning nozzle to the winding up roller is 6cm, and winding speed is 3cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 2mm, and exit width is 100 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is a straight line; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 5
With mass percent concentration is that 30% regenerated silk fibroin water solution is a spinning solution, under 30 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 0.5 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt wet spinning process, in 80% ethanolic solution, be solidified into the silk and the top roll of reeling; Winding speed is 1cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 10 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 2mm, and exit width is 10 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of second order exponential function curve and straight line; Described exponential function formula is: R (x)=ae
Bx+ ce
DxA=-0.004886 wherein, b=0.0003718, c=53.52, d=-9.989*10
-5The length ratio of described curve and straight line is 2: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 6
With mass percent concentration is that 60% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 10 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt wet spinning process, in 80% ethanolic solution, be solidified into the silk and the top roll of reeling; Winding speed is 5cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 500 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 1mm, and exit width is 500 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of hyperbolic function curve and straight line; Described hyperbolic function formula is: R (x)=1/2 (e
x-e
-x); The length ratio of described curve and straight line is 1: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 7
With mass percent concentration is that 45% regenerated silk fibroin water solution is a spinning solution, under 10 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 5 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt wet spinning process, in 80% ethanolic solution, be solidified into the silk and the top roll of reeling; Winding speed is 3cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 500 μ m, and exit width is 250 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is a single order exponential function curve; Described exponential function formula is: R (x)=e
A*ln (x)A=0.0125 wherein; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 8
With mass percent concentration is that 55% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions, spinning solution is injected micro-fluid chip with the flow velocity of 8 μ L/min, extrude the back by the outlet of the microfluidic channel of micro-fluid chip and adopt wet spinning process, in 80% ethanolic solution, be solidified into the silk and the top roll of reeling; Winding speed is 3cm/s.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 2mm, and exit width is 100 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is a straight line; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 9
With mass percent concentration is that 30% regenerated silk fibroin water solution is a spinning solution, under 30 ℃ temperature conditions spinning solution is injected micro-fluid chip with the flow velocity of 0.5 μ L/min.The metal catheter that will link to each other with the micro-fluid chip inlet is connected anodal, and the sample reception plate earthing is carried out electrostatic spinning by the micro-fluid chip outlet under electric field, and voltage is that 30kV, receiving range are 6cm.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 10 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 2mm, and exit width is 10 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of second order exponential function curve and straight line; Described exponential function formula is: R (x)=ae
Bx+ ce
DxA=-0.004886 wherein, b=0.0003718, c=53.52, d=-9.989*10
-5The length ratio of described curve and straight line is 2: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 10
With mass percent concentration is that 60% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions spinning solution is injected micro-fluid chip with the flow velocity of 10 μ L/min.The metal catheter that will link to each other with the micro-fluid chip inlet is connected anodal, and the sample reception plate earthing is carried out electrostatic spinning by the micro-fluid chip outlet under electric field, and voltage is that 30kV, receiving range are 6cm.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 500 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and throat width is 1mm, and exit width is 500 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is the combination of hyperbolic function curve and straight line; Described hyperbolic function formula is: R (x)=1/2 (e
x-e
-x); The length ratio of described curve and straight line is 1: 3; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 11
With mass percent concentration is that 45% regenerated silk fibroin water solution is a spinning solution, under 10 ℃ temperature conditions spinning solution is injected micro-fluid chip with the flow velocity of 5 μ L/min.The metal catheter that will link to each other with the micro-fluid chip inlet is connected anodal, and the sample reception plate earthing is carried out electrostatic spinning by the micro-fluid chip outlet under electric field, and voltage is that 30kV, receiving range are 6cm.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 500 μ m, and exit width is 250 μ m.Described microfluidic channel, the trace that its two sides and horizontal plane intersect is a single order exponential function curve; Described exponential function formula is: R (x)=e
A*ln (x)A=0.0125 wherein; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
Embodiment 12
With mass percent concentration is that 55% regenerated silk fibroin water solution is a spinning solution, under 20 ℃ temperature conditions spinning solution is injected micro-fluid chip with the flow velocity of 8 μ L/min.The metal catheter that will link to each other with the micro-fluid chip inlet is connected anodal, and the sample reception plate earthing is carried out electrostatic spinning by the micro-fluid chip outlet under electric field, and voltage is that 30kV, receiving range are 6cm.
Described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The degree of depth c of described microfluidic channel whole process equates to be 100 μ m, and described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth, and as illustrated in fig. 1 and 2, throat width is 2mm, and exit width is 100 μ m.Described microfluidic channel, the trace that one side and horizontal plane intersect is a straight line; The trace that another side and horizontal plane intersect is a single order exponential function curve; Described exponential function formula is: R (x)=e
A*ln (x)A=0.0125 wherein.
Claims (10)
1. micro-fluid chip, comprise substrate and diaphragm, fluted on the diaphragm, reeded of diaphragm and substrate are fitted and are formed microfluidic channel, it is characterized in that: the degree of depth c of described microfluidic channel whole process equates that described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth.
2. a kind of micro-fluid chip according to claim 1 is characterized in that, the two sides of described microfluidic channel are about microfluidic channel central axis symmetry; Described microfluidic channel, the trace that its two sides and horizontal plane intersect are a kind of or its combination in exponential function curve, hyperbolic function curve or the straight line.
3. a kind of micro-fluid chip according to claim 2 is characterized in that, described exponential function is the second order exponential function.
4. a kind of micro-fluid chip according to claim 1 is characterized in that, described degree of depth c is 10 μ m～500 μ m; Described microfluidic channel, throat width are 500 μ m～2mm, and exit width is 10 μ m～500 μ m.
5. the spinning process of a micro-fluid chip, it is characterized in that: the inlet that spinning solution is injected the microfluidic channel of micro-fluid chip, flow out by the outlet of microfluidic channel at last, in air, carry out dry-spinning, or in the polymer coagulating bath, carry out wet spinning, or under the high voltage electric field condition, carry out electrostatic spinning; The degree of depth c of described microfluidic channel whole process equates that described microfluidic channel width b is reduced gradually continuously by the outlet that enters the mouth.
6. the spinning process of a kind of micro-fluid chip according to claim 5 is characterized in that, described micro-fluid chip comprises substrate and diaphragm, and is fluted on the diaphragm, and reeded of diaphragm and substrate are fitted and formed microfluidic channel; The two sides of described microfluidic channel are about microfluidic channel central axis symmetry.
7. according to the spinning process of claim 5 or 6 described a kind of micro-fluid chips, it is characterized in that, described microfluidic channel, the trace that its two sides and horizontal plane intersect is a kind of or its combination in exponential function curve, hyperbolic function curve or the straight line.
8. the spinning process of a kind of micro-fluid chip according to claim 7 is characterized in that, described exponential function is the second order exponential function.
9. the spinning process of a kind of micro-fluid chip according to claim 5 is characterized in that, described degree of depth c is 10 μ m～500 μ m; Described microfluidic channel, throat width are 500 μ m～2mm, and exit width is 10 μ m～500 μ m.
10. the spinning process of a kind of micro-fluid chip according to claim 5 is characterized in that, the injection rate that described spinning solution injects microfluidic channel is 0.5～10 μ L/min; When carrying out dry-spinning, the air-gap from the spinning nozzle to the winding up roller is 1～30cm, and winding speed is 1～5cm/s.
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