CN102160531A - Temporary culture and ripening promotion method for Octopus vulgaris parents and device used in same - Google Patents
Temporary culture and ripening promotion method for Octopus vulgaris parents and device used in same Download PDFInfo
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- CN102160531A CN102160531A CN201110132223XA CN201110132223A CN102160531A CN 102160531 A CN102160531 A CN 102160531A CN 201110132223X A CN201110132223X A CN 201110132223XA CN 201110132223 A CN201110132223 A CN 201110132223A CN 102160531 A CN102160531 A CN 102160531A
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- 241000238414 Octopus vulgaris Species 0.000 title abstract description 10
- 230000005070 ripening Effects 0.000 title abstract description 8
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 8
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- 235000021122 unsaturated fatty acids Nutrition 0.000 claims description 5
- 150000004670 unsaturated fatty acids Chemical class 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 4
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a temporary culture and ripening promotion method for Octopus vulgaris parents, and the method is characterized by comprising the following steps: firstly culturing Octopus vulgaris in a temporary culture pond which is provided with a triangular prism-shaped Octopus vulgaris nest, raising water temperature to 24 DEG C, and controlling the salinity of sea water within a range from 3% to 3.2% and the light intensity within a range from 500 Lx to 800 Lx; and feeding ripening promotion feed during the ripening promotion stage, and feeding nest sealing materials after Octopus vulgaris is cultured for 25 to 30 days. The method is practical, stable and effective. The Octopus vulgaris nest used in the invention is made by sintering light-weight heat-insulation clay, and is corrosion-resistant, wear-resistant, durable, low in price and convenient in large-scale promotion and application. The Octopus vulgaris nest is constituted by three sheets and is convenient in assembly and disassembly and convenient in cleaning and storage. By utilizing the method, the Octopus vulgaris individuals can be prevented from fighting each other, the natural growth environments of sea, which is suitable for the parents to lay eggs and protect the eggs is, is simulated. Besides, the side connecting plates of the Octopus vulgaris nest are distributed in a concave-convex alternate manner, so that the parents can lay egg strings on the convex parts, thereby reducing the stacking density of the egg strings and increasing the egg hatchability.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of true octopus parent and support accelerating method and equipment therefor temporarily.
Technical background
True octopus (Octopus vulgaris) belongs to Mollusca, Cephalopoda, Octopodida, octopus section, octopus genus, be commonly called as and be octopus, inhabit shallow sea grit or cay place more, happiness food shell-fish and bivalve shellfish, be widely distributed in temperature, tropical marine site, mainly be distributed in southeastern coast marine sites such as Zhoushan, Xiapu, Pingtan, Xiamen, Shantou in China.True octopus edible part is high, can eat or drying its delicious meat raw, comprehensive nutrition, protein, content of highly unsaturated fatty acids enrich, and the true octopus consumption figures in ground such as Fujian, Guangdong, Korea S, Japan are huge, and the exhausted day by day contradiction of the market demand and resource is given prominence to day by day.
True octopus is a kind of economic kind of culturing potentiality and development prospect that has very much, has characteristics such as fast growth, culture-cycle weak point, nutritive value height.Tens countries such as Japan, Spain, Portugal have successively carried out accelerating, the work of seed breeding correlation test of supporting temporarily, and have obtained certain achievement, and China's research in this respect is backward relatively, the related data scarcity.
Present scientific research personnel both domestic and external never solves big, low, the nonsynchronous problem of paedomorphosis of archiblast amount of true octopus fish brood amount individual difference, and true octopus parent to support the accelerating method temporarily be to carry out that the scale seed is healthyly cultivated, propagation is released and the important previous work of breeding production.True octopus parent grows and the important factor of gonad maturity as influencing, and does not see relevant report for the research of accelerating bait.
Summary of the invention
The purpose of this invention is to provide a kind of true octopus parent and support accelerating method and equipment therefor temporarily, true octopus parent fish brood amount is low under the solution existence conditions, and the asynchronous and low inferior problem of archiblast amount of paedomorphosis is to remedy the deficiencies in the prior art.
Method of the present invention is characterized in that at first very high and steep putting in a suitable place to breed in input being had in the storage pond, holding pond of triangular prism shaped octopus nest, rising water temperature to 24 ℃, and seawater salinity is 30~32 ‰, light intensity is 500~800Lx; The accelerating bait of throwing something and feeding during accelerating after putting very high and steep 25~30 days in a suitable place to breed, is thrown in envelope nest thing, finishes the accelerating process.
Described accelerating bait is nearly side crab of fine hair and/or Ruditapes philippinarum; The feeding volume of every day is 12%~16% of a true octopus parent weight.
In order to shorten the cycle of accelerating the ripening, to increase fish brood amount, the wet bait of the biology of during accelerating the ripening, also throwing something and feeding, the wet bait of described biology includes the component of following percentage by weight: highly unsaturated fatty acid 25~35%, vitamin C 8~15%, active bacteria formulation 25~35%, saccharicter-penin 16~20%, wherein active bacteria formulation is the bacillus subtilis active bacteria formulation.Biological wet bait was thrown something and fed once in per two days, and feeding volume is 2.4%~3.2% of a true octopus parent weight.
The true octopus female-male proportion of throwing in storage pond, holding pond is 1~2: 1.
The PHMB final concentration was 6~10 μ g/ml with PHMB full pool spilling head once sterilizing in per 10 days in the accelerating phase.
Implement the used octopus nest of the inventive method, for triangular prism shaped, assembled by a bottom surface knot plate and two lateral junction plates, wherein the inner surface of lateral junction plate is concavo-convex and distributes alternately.
Build and split cleaning for convenience, on the knot plate of bottom surface, be provided with the groove that is used to install the lateral junction plate.
Described bottom surface knot plate and lateral junction plate are formed by vitrified bond.
The present invention has set up the method that a kind of true octopus parent is supported accelerating temporarily, can effectively carry out the extensive parent of true octopus and support accelerating work temporarily, lays the foundation for further carrying out the work of true octopus larval culture.Method practice of the present invention is strong, effectively stable.And octopus nest of the present invention is formed by light thermal-insulation vitrified bond, anticorrosive, resistance to wear, durable, cheap, be convenient to large-scale promotion application.The octopus nest is combined by three thin plates, conveniently builds fractionation and cleaning and deposits, and not only makes between true octopus individuality and avoids mutual fight, and simulated marine site self-sow environment, and suitable parent is laid eggs and protected ovum.Concavo-convex the distributing alternately of octopus nest lateral junction plate impels parent that the ovum string is originated on the bossing simultaneously, reduces ovum string bulk density, improved incubation rate.
Description of drawings
Fig. 1: the bottom surface knot plate schematic diagram of octopus nest of the present invention.
Fig. 2: the lateral junction intralamellar part structural representation of octopus nest of the present invention.
Fig. 3: octopus nest overall structure sectional view of the present invention.
Wherein: 1, bottom surface knot plate 2, lateral junction plate 3, groove.
Embodiment
In order to reduce accusing each other between the true octopus in the accelerating process, according to true octopus happiness troglodytism habit, the present invention throws in the octopus nest in storage pond, holding pond, used octopus nest adopts natural material to make, but simulating nature egg-laying environment, avoid accusing each other between parent, laying eggs, protect ovum for parent provides a stable environment.And the concaveconvex structure of octopus nest lateral junction plate can effectively divide the laying off eggs population density, improves young incubation rate and quality.
Octopus nest of the present invention is built by the soft clay sintered plate, is the triangular prism structure, and bottom surface knot plate 1 size is 27cm * 24cm (long * wide), and thick is 1cm, and respectively there is the groove 3 of an outer ledge projection both sides, long limit of bottom surface knot plate 1, and width is 1cm.Lateral junction plate 2 sizes are 27 * 16cm (long * wide), and its medial surface is concavo-convex to distribute alternately, the wide 0.5cm of recess (Fig. 2, down together), the wide 1.0cm of protuberance.Groove 3 medial surfaces knot plate 2 upper ends that two lateral junction plates 2 are installed on bottom surface knot plate 1 stick with glue, and make its combination fastening (Fig. 3).
Above-mentioned octopus nest is put in the storage pond, holding pond, and the quantity of octopus nest will be more than the very high and steep parent's who throws in quantity, and double-deck sunshade net is put up in the storage pond, holding pond top, and the adjusting intensity of illumination is 500~800Lx.Storage pond, holding pond injects sand filter water, and originally water temperature is 18 ℃, and salinity is 30~32 ‰, and aeration moves into storage pond, holding pond with true octopus parent two days later, chooses that body surface is complete, healthy looking, strong, the full individuality of trunk of vigor, and female-male proportion is 1~2: 1.Begin to heat up after supporting a week temporarily, heated up 1 ℃, and be warming up to 24 ℃ in per two days.
The selection of bait has important effect for very high and steep accelerating the ripening, and method of the present invention is thrown something and fed the wild bait of very high and steep happiness such as the nearly side crab of fine hair, Ruditapes philippinarum food as bait in the phase of accelerating the ripening, and the biological wet bait of throwing something and feeding is simultaneously assisted accelerating.The total input of bait is 12%~16% of a true octopus parent weight, and wherein biological wet bait accounts for 20% (true octopus parent weight 2.4%~3.2%) of total bait.16:00 bait throwing in afternoon every day once, day feeding volume is there to be residual bait degree of being every day slightly.
Biological wet bait includes the component of following percentage by weight: highly unsaturated fatty acid 25~35%, vitamin C 8~15%, active bacteria formulation 25~35%, saccharicter-penin 16~20%, wherein active bacteria formulation is the bacillus subtilis viable bacteria, and technical research institute provides by the prosperous generation thing in Cangzhou.Above component is taken by weighing according to weight ratio, next bonding with adhesive commonly used in the breeding bait then, for example with mixing the nearly side crab of meat ball crab meat, making length is 4-6cm column bait, throw something and feed in the storage pond, holding pond, threw something and fed once in two days, feeding volume is 2.4%~3.2% of a true octopus parent weight.
To very high and steeply put in a suitable place to breed at storage pond, holding pond after 25~30 days, can observe and close on the parent of laying eggs the phenomenon that drags the octopus nest is arranged, should in time throw in envelope nest thing to spawning pond this moment, quantity is 7~10 times of octopus nest, envelope nest thing is made by materials such as the marble of smashing, granite or fragments of brick, and size is 45~85 * 10
-6m
3Render to after the washing and sterilizing in the pond, parent drags envelope nest thing and seals the nest mouth, and stable environment is provided for laying eggs.
In order to prevent to occur bacterium in the water body, the per 10 days PHMB that splash a time, the final concentration that makes PHMB in the spawning pond is 6~10 μ g/ml.
True octopus is higher to water quality requirement, and a day water exchange capacity reaches 200%~300%.Grasp slow-motion when changing water and go out principle slowly, water temperature change should be controlled at ± 2 ℃, change the excessive bad reaction that causes with water proof environment.
GSI is the index of true octopus maturity of fish gonads, by measuring GSI numerical value, selects true octopus parent.The selection criteria of parent: body surface is complete, healthy looking, trunk is full, vigor is stronger, and more than the long 54.0cm of female body body, more than the body weight 550.0g, GSI is greater than 15%, more than the long 60.0cm of male body, more than the body weight 630.5g.
The invention will be further described below in conjunction with example:
Transport true octopus 143 from marine site, Liaanjiang county, Fujian in May, 2010, and 16~18 ℃ of water temperatures are transported to experiment base, Yantai under salinity 28~30 conditions, survive 105, and body weight is 249g~1478g, average 660.1g.Parent is divided into three groups, places 36 of parents for every group.Do not throw in the octopus nest for first group, the biological wet bait of not throwing something and feeding is thrown in the octopus nest for second group, and the biological wet bait of not throwing something and feeding is thrown in the octopus nest for the 3rd group, the biological wet bait of throwing something and feeding.
First group of test: do not throw in the octopus nest, the biological wet bait of not throwing something and feeding
18m
318 ℃ of storage pond, holding pond water temperatures, pH value 8.0~8.4, salinity 30~32 ‰, intensity of illumination 500~800Lx.Do not throw in the octopus nest in the storage pond, holding pond, place 36 of parents, wherein female 20, male 16, and place shelters such as PCV pipe, hollow brick, fragment of brick.True octopus happiness food shell-fish and bivalve shellfish.Afternoon every day the 16:00 accelerating bait (Ruditapes philippinarum) of throwing something and feeding, a day feeding rate is 12%~16% of a true octopus parent weight.Begin to heat up after supporting a week temporarily, heated up 1 ℃ in per 2 days, till 24 ℃.During supporting temporarily, true octopus is fought mutually, the body surface breakage, and the part peduncle breaks.True octopus is dead 6 during supporting temporarily, accounts for totally 16.67%, begins to lay eggs after supporting 38~42d temporarily.
Spawning pond is clean with flushing with clean water, and again with 30mg/L potassium permanganate full pool spilling head sterilization 20min, again with at the bottom of bleaching powder sterilization pool wall and the pond, the fouling product on the pool wall particularly uses after clean every other day with flushing with clean water at last after rinsing well.21.8~23.4 ℃ of spawning pond water temperatures, salinity 30~33, dissolved oxygen 3~4mg/L, the sunshade net is put up in the top, pond, regulates light intensity 500~800Lx.Earlier the spawning pond sterilization is cleaned, then parent is put into spawning pond.True octopus is movable frequent before laying eggs, more than 280~310 ten thousand on 14 female octopus common property ovum, average every egg laying amount 20~220,000, parent is subject to its ectocine during protecting ovum, and physical demands is serious, and it is seriously lax to protect ovum later stage body surface, eyes are partly opened, mobility a little less than, 5 individual deaths are arranged.
Second group of experiment: throw in the octopus nest, the biological wet bait of not throwing something and feeding
The rearmounted sand filter water of storage pond, holding pond strict sterilization, 18 ℃ of water temperatures, pH value 8.0~8.4, salinity 30~32 ‰, more than the dissolved oxygen 5mg/L, the top, pond is with the processing of black sunshade net, and intensity of illumination is controlled at 500~800Lx.18m
3Place 36 of parents, 41 in octopus nest, two octopus nest spacings, 30~40cm in the storage pond, holding pond.Afternoon every day the 16:00 accelerating bait Ruditapes philippinarum of throwing something and feeding, a day feeding rate is 12%~16%.Begin to heat up after supporting a week temporarily, heated up 1 ℃ in per 2 days, till 24 ℃.During supporting temporarily, true octopus is many in octopus nest separately and the near zone activity, and the situation of fighting obviously reduces, and the peduncle phenomenon reduces, and true octopus is dead 2 during supporting temporarily, accounts for overall 5.56%.Begin to lay eggs after supporting 36~41d temporarily.28 of parents are selected in the test of this group, and wherein female individuals 16,12 of males.
True octopus is movable frequent before laying eggs, and drags other octopus nest phenomenons, the envelope nest thing that throw in sterilization and clean this moment in spawning pond, and envelope nest thing is good with crushed stone.True octopus drags envelope nest thing the nest mouth is sealed, and this phenomenon is called " envelope nest ".The envelope nest is the important symbol of true octopus before laying eggs, more than 410~450 ten thousand on 16 female octopus common property ovum of this experiment, on average every egg laying amount 25~280,000.Parent is seldom gone out during protecting ovum, and octopus nest two ends are sealed under the effect of envelope nest body, and ectocine is less, protects ovum later stage parent physique and descends serious.
The octopus nest is thrown in the 3rd group of experiment, the biological wet bait of throwing something and feeding
The rearmounted sand filter water of storage pond, holding pond strict sterilization, 18 ℃ of water temperatures, pH value 8.0~8.4, salinity 30~32, more than the dissolved oxygen 5mg/L, the top, pond is with the processing of black sunshade net, and intensity of illumination is controlled at 500~800Lx.18m
3Place 36 of parents, 41 in octopus nest in the storage pond, holding pond.
The experiment of this group is except throwing something and feeding accelerating bait (Ruditapes philippinarum) also as first and second group, the wet bait of the biology of also throwing something and feeding.Biological wet bait is made of following component in parts by weight: highly unsaturated fatty acid 15~25, vitamin C 4~10, nearly side crab 35~50, active bacteria formulation 5~10, saccharicter-penin 5~10.16:00 bait throwing in afternoon every day once, day feeding volume is 12%~16% of true octopus parent weight so that residual bait degree of being to be arranged every day slightly, wherein biological wet bait accounts for 20% of total bait.The morning on the secondth, 9:00 removed residual bait at the bottom of the pond, if the bait residue is more, threw something and fed next time and will take the circumstances into consideration decrement.In case find true octopus reversal peduncle phenomenon is arranged, the bait deficiency is described, need to increase daily ration, feeding quantity.Different bait cooperate throws something and feeds, wherein biological wet throwing something and feeding once in one week of bait.
More than 800~1,100 ten thousand on 34 female octopus common property ovum of this group, average every egg laying amount 27~330,000.
Learn that by above test throw in second group of triangular prism octopus nest and parent behavior of fighting during supporting temporarily of the 3rd group and reduce, the injured degree of body surface lowers, disconnected wrist phenomenon is lighter.Protect during the ovum, octopus nest more parent provides a stable spawning place, and it is less to be affected by the external environment, and reduces the consumption of parent energy, helps improving the quality of protecting ovum.The design of octopus nest concaveconvex structure helps the flowability of water between the ovum group, reduces growing of microorganism such as bacterium, improves ovum group's quality.And the 3rd group of biological wet bait of throwing something and feeding, parent fish brood amount gap is less, and every individual bosom ovum is about 300,000, significantly is higher than other two groups, and ovum is grown up in other two groups, and the archiblast amount obviously improves, and young hatching is more synchronous, and incubation rate is higher.
Claims (10)
1. a true octopus parent is supported the accelerating method temporarily, it is characterized in that at first very high and steep putting in a suitable place to breed in input being had in the storage pond, holding pond of triangular prism shaped octopus nest, and rising water temperature to 24 ℃, seawater salinity is 30~32 ‰, light intensity is 500~800Lx; The accelerating bait of throwing something and feeding during accelerating after putting very high and steep 25~30 days in a suitable place to breed, is thrown in envelope nest thing.
2. accelerating method as claimed in claim 1 is characterized in that above-mentioned accelerating bait is nearly side crab of fine hair and/or Ruditapes philippinarum.
3. accelerating method as claimed in claim 1 or 2 is characterized in that the feeding volume of above-mentioned accelerating bait every day is 12%~16% of a true octopus parent weight.
4. accelerating method as claimed in claim 1, biological wet bait is characterized in that throwing something and feeding during accelerating, the wet bait of described biology includes the component of following percentage by weight: highly unsaturated fatty acid 25~35%, vitamin C 8~15%, active bacteria formulation 25~35%, saccharicter-penin 16~20%, wherein active bacteria formulation is the bacillus subtilis viable bacteria.
5. accelerating method as claimed in claim 4 is characterized in that the wet bait of above-mentioned biology threw something and fed once in per two days, and feeding volume is 2.4%~3.2% of a true octopus parent weight.
6. accelerating method as claimed in claim 1 is characterized in that the true octopus female-male proportion of throwing in the above-mentioned storage pond, holding pond is 1~2: 1.
7. accelerating method as claimed in claim 1 is characterized in that in the above-mentioned accelerating phase per 10 days with PHMB full pool spilling head once sterilizing, and the PHMB final concentration is 6~10 μ g/ml.
8. the octopus nest that uses in the described method of claim 1 for triangular prism shaped, is assembled by a bottom surface knot plate (1) and two lateral junction plates (2), and wherein the inner surface of lateral junction plate (2) is concavo-convex and distributes alternately.
9. octopus nest as claimed in claim 8 is characterized in that described bottom surface knot plate (1) is provided with the groove (3) that is used to install lateral junction plate (2).
10. octopus nest as claimed in claim 8 is characterized in that described bottom surface knot plate (1) and lateral junction plate (2) are formed by vitrified bond.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102550460A (en) * | 2012-02-15 | 2012-07-11 | 山东省海洋水产研究所 | Artificial propagation method of Octopus ocellatus in outdoor earth pond |
CN102669021A (en) * | 2012-05-01 | 2012-09-19 | 中国海洋大学 | Method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and device for method |
CN104106485A (en) * | 2014-07-21 | 2014-10-22 | 江苏虎石潭农业发展有限公司 | Triangle spreading type ovipositor for adhesive egg fish |
CN106719178A (en) * | 2016-12-05 | 2017-05-31 | 浙江海洋大学 | A kind of cephalopodous artificial ripening method |
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Cited By (7)
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CN102550460A (en) * | 2012-02-15 | 2012-07-11 | 山东省海洋水产研究所 | Artificial propagation method of Octopus ocellatus in outdoor earth pond |
CN102550460B (en) * | 2012-02-15 | 2013-07-31 | 山东省海洋水产研究所 | Artificial propagation method of Octopus ocellatus in outdoor earth pond |
CN102669021A (en) * | 2012-05-01 | 2012-09-19 | 中国海洋大学 | Method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and device for method |
CN102669021B (en) * | 2012-05-01 | 2013-11-27 | 中国海洋大学 | Method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and device for method |
CN104106485A (en) * | 2014-07-21 | 2014-10-22 | 江苏虎石潭农业发展有限公司 | Triangle spreading type ovipositor for adhesive egg fish |
CN106719178A (en) * | 2016-12-05 | 2017-05-31 | 浙江海洋大学 | A kind of cephalopodous artificial ripening method |
CN106719178B (en) * | 2016-12-05 | 2020-06-09 | 浙江海洋大学 | Artificial ripening method for cephalopods |
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