CN102115717B - Low nucleic acid yeast product, preparation method thereof and weight-reducing product comprising same - Google Patents
Low nucleic acid yeast product, preparation method thereof and weight-reducing product comprising same Download PDFInfo
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- CN102115717B CN102115717B CN2009102158911A CN200910215891A CN102115717B CN 102115717 B CN102115717 B CN 102115717B CN 2009102158911 A CN2009102158911 A CN 2009102158911A CN 200910215891 A CN200910215891 A CN 200910215891A CN 102115717 B CN102115717 B CN 102115717B
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- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 129
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 57
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 57
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 12
- 230000001105 regulatory effect Effects 0.000 claims abstract description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000011575 calcium Substances 0.000 claims abstract description 6
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 6
- 229910052742 iron Inorganic materials 0.000 claims abstract description 6
- 239000011701 zinc Substances 0.000 claims abstract description 6
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 109
- 238000001035 drying Methods 0.000 claims description 10
- 238000009413 insulation Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 10
- 201000005569 Gout Diseases 0.000 abstract description 9
- 230000000050 nutritive effect Effects 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 7
- 230000035764 nutrition Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 abstract 1
- 230000004130 lipolysis Effects 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 14
- 235000013305 food Nutrition 0.000 description 9
- 230000037213 diet Effects 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 8
- 239000002002 slurry Substances 0.000 description 7
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 6
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 6
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 6
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 229940116269 uric acid Drugs 0.000 description 6
- 238000005507 spraying Methods 0.000 description 5
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 4
- PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 description 3
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- UZWMCCLZMHPPKW-UHFFFAOYSA-L calcium;2-oxopropanoate Chemical compound [Ca+2].CC(=O)C([O-])=O.CC(=O)C([O-])=O UZWMCCLZMHPPKW-UHFFFAOYSA-L 0.000 description 3
- 229960000304 folic acid Drugs 0.000 description 3
- 235000019152 folic acid Nutrition 0.000 description 3
- 239000011724 folic acid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000001968 nicotinic acid Nutrition 0.000 description 3
- 229960003512 nicotinic acid Drugs 0.000 description 3
- 239000011664 nicotinic acid Substances 0.000 description 3
- 229940055726 pantothenic acid Drugs 0.000 description 3
- 235000019161 pantothenic acid Nutrition 0.000 description 3
- 239000011713 pantothenic acid Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 235000019166 vitamin D Nutrition 0.000 description 3
- 239000011710 vitamin D Substances 0.000 description 3
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 2
- 229930003779 Vitamin B12 Natural products 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019155 vitamin A Nutrition 0.000 description 2
- 239000011719 vitamin A Substances 0.000 description 2
- 235000019163 vitamin B12 Nutrition 0.000 description 2
- 239000011715 vitamin B12 Substances 0.000 description 2
- 235000019158 vitamin B6 Nutrition 0.000 description 2
- 239000011726 vitamin B6 Substances 0.000 description 2
- 229940045997 vitamin a Drugs 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- -1 hydrogen sodium oxide Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/08—Reducing the nucleic acid content
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to a low nucleic acid yeast product and a preparation method thereof. The low nucleic acid yeast product comprises 45 percent to 55 percent of protein, 0.05 percent to 1.0 percent of nucleic acid, 100ppm to 5,000ppm of calcium, 30ppm to 1,000ppm of iron, and 30ppm to 1,000ppm of zinc. The invention further relates to the preparation method of the low nucleic acid yeast product. The preparation method comprises the following steps: yeast is mixed with water into a solution with a mass percentage concentration of 5 percent to 10 percent; the pH (hydrogen ion concentration) of the solution is regulated to a value between 6.0 and 10.0; the heat of the yeast solution is preserved for 1 to 6 hours at a temperature of 60 DEG C to 100 DEG C; the pH of the yeast solution is further regulated to a value between 5.0 and 7.0; the yeast solution is separated, washed and dried; and then the low nucleic acid yeast product is acquired. The method disclosed by the invention has the advantages that the nucleic acid content in the yeast is reduced simply, conveniently and effectively, namely the danger of gout caused by eating the yeast or relevant products produced by using the yeast as a raw material is reduced, and the nutritive value in the original yeast is preserved. The invention further relates to a weight-reducing product comprising the low nucleic acid yeast. The weight-reducing product has the advantages of low calorie, high nutrition, safety and effectiveness, and can promote lipolysis.
Description
Technical field
The present invention relates to a kind of low nucleic acid yeast product and preparation method thereof, and a kind of diet products that comprise this low nucleic acid yeast.
Background technology
Yeast begins to come into daily life as a kind of food, and increasing yeast product is come out.Now most of Yeast food is to produce beer yeast slurry after the beer as raw material on the market, is developed to different food.Prior art mainly is that to handle then convection drying just edible to the people by cereuisiae fermentum being carried out debitterize, and perhaps some mineral substance of reinforcement, trace element before dry are made nutrient prime replenisher.
A lot of people cause gout after reflecting edible this series products easily in recent years, and the major ingredient that wherein causes gout is nucleic acid.Yeast amplifying nucleic acid content generally about 5%, edible be the product of main raw material with this primary yeast after, cause gout easily.Nucleic acid class protective foods is declared and is evaluated regulation " ' being not suitable for the crowd ' in nucleic acid class protective foods specification sheets and the label should clearly mark out ' patient with gout ' except pressing protective foods relevant regulations mark ".
Will be behind the beer yeast slurry convection drying edible to the people, or be that carrier is made various nutrient prime replenishers with the yeast.
To nucleic acid and the not control of nucleic acid content of yeast, cause causing gout behind some edible this series products in the prior art, bring very big misery to the human consumer.
Dense salt method, diluted alkaline method can be come out the nucleic acid extraction in the yeast, but these methods cause the nutritive loss of yeast serious, and the yeast of production does not have value edible or health care.
Summary of the invention
The technical problem that the present invention mainly solves is the nucleic acid that reduces in the yeast, keeps the nutritive value of protein, calcium, iron, zinc and yeast in the yeast.
The preparation method of low nucleic acid yeast product provided by the invention is to be raw material with face with yeast, yeast saccharomyces cerevisiae, the cereuisiae fermentum beer yeast slurry of debitterize (or through), the permeability principle is dissolved in the nucleic acid in the yeast in the water to utilize yeast yeast cells wall under finite concentration, temperature and pH to have preferably, removes the nucleic acid in the yeast by separating, washing but does not lose protein in the yeast.Drop to 0.1~1.0% through yeast amplifying nucleic acid after this art breading by original about 5%, well below existing yeast amplifying nucleic acid, keep nutritive elements such as protein in this yeast, calcium, iron, zinc simultaneously.The food of producing with this yeast does not have denseer yeast flavour, product protein height, to human body safety (can not cause gout).Concrete process flow sheet as shown in Figure 1.
The main component weight percentage of low nucleic acid yeast provided by the present invention is: protein 45~55%, nucleic acid (in RNA) 0.05~1.0%, calcium 100~5000ppm, iron 30~1000ppm, zinc 30~1000ppm.This yeast is a kind of dry yeast, and smell is lighter.
The preparation method of a kind of low nucleic acid yeast provided by the present invention, concrete steps comprise:
1) gets the yeast water and be mixed with the solution that mass percent concentration is 5-10%, preferred concentration 7~10%, optimum 8%;
2) regulating step 1) the gained yeast soln is to pH=6.0-10.0, preferred pH=7.0-9.0, most preferably pH=8.5.
3) with step 2) the gained yeast soln was 60-100 ℃ of insulation 1-6 hour, and preferred temperature 80-100 ℃, soaking time 2-4 hour, most preferably 95 ℃ were incubated 3 hours;
4) re-adjustment step 3) gained yeast soln is to pH=6.0;
5) separate washing, drying step 4) the gained yeast soln, obtain low nucleic acid yeast.
Step 2 wherein), the acid of regulating pH in the step 4) can be selected sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid etc. for use, alkali available hydrogen sodium oxide, potassium hydroxide etc.
Particularly, the separating device that the separating step in the described step 5) can be chosen prior art as the case may be wantonly carries out, as stacked separating machine.
Method of the present invention is simple and feasible, cost is low, utilize method of the present invention to reduce yeast amplifying nucleic acid content effectively simultaneously, i.e. it is the danger of the related products initiation gout of raw material production that edible this yeast of reduction reaches with this yeast, and has kept the nutritive value in original yeast.The low nucleic acid yeast product of the present invention preparation is still complete yeast cell, has kept the nutritive substance in the cell with effective.
Another technical problem that the present invention solves has provided a kind of safe and effective diet products,
Diet products involved in the present invention are to be that raw material is aided with CALCIUM PYRUVIC, L-carnitine with low nucleic acid yeast, and forced for vitamins B1, B2, B6, B12, folic acid, nicotinic acid, pantothenic acid, vitamin A, vitamins C, vitamins D etc. mix simultaneously.
The main component weight percentage of these diet products is: low nucleic acid yeast content is 70~99%, CALCIUM PYRUVIC content 0.1~10%, L-carnitine content 0.1~15%, the content of nutritive element is: VB11 0~1000ppm, Wei ShengsuB2 10~1000ppm, vitamin B6 10~1000ppm, vitamin B12 0.1~50ppm, folic acid 10~1000ppm, nicotinic acid 10~5000ppm, pantothenic acid 50~5000ppm, retinol1 0~500ppm, vitamins C 1000~5000ppm, vitamins D 0.1~10ppm.
Diet food of the present invention is that a kind of nutrition low in calories, high also has the fat-splitting effect of promotion simultaneously, is a safe and effective diet food.
Description of drawings
Fig. 1 prepares the main technique schema of low nucleic acid yeast product for the present invention.
Embodiment
Describe the present invention by the following examples in detail.
One, the low nucleic acid yeast product of preparation
Embodiment 1
Get the Angel yeast saccharomyces cerevisiae that Angel Yeast Co.,Ltd produces, add deionized water, be mixed with mass percent concentration and be 5% solution; Regulate this yeast soln to pH=6.0 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 1 hour at 60 ℃; This yeast soln of re-adjustment is to pH=5.0; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out spraying drying, obtains low nucleic acid yeast sample 1
#
Embodiment 2
Get the Angel cereuisiae fermentum that Angel Yeast Co.,Ltd produces, add deionized water, be mixed with mass percent concentration and be 7% solution; Regulate this yeast soln to pH=9 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 4 hours at 80 ℃; This yeast soln of re-adjustment is to pH=6.0; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out roller drying, obtains low nucleic acid yeast sample 2
#
Embodiment 3
Get the Angel face yeast that Angel Yeast Co.,Ltd produces, the adding deionized water is mixed with mass percent concentration and is 5% solution; Regulate this yeast soln to pH=6.0 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 1 hour at 60 ℃; This yeast soln of re-adjustment is to pH=7.0; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out spraying drying, obtains low nucleic acid yeast sample 1
#
Embodiment 4
Get the Angel face yeast that Angel Yeast Co.,Ltd produces, the adding deionized water is mixed with mass percent concentration and is 8% solution; Regulate this yeast soln to pH=8.5 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 3 hours at 95 ℃; This yeast soln of re-adjustment is to pH=6.5; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out spraying drying, obtains low nucleic acid yeast sample 4
#
Embodiment 5
Get the Angel face yeast that Angel Yeast Co.,Ltd produces, the adding deionized water is mixed with mass percent concentration and is 10% solution; Regulate this yeast soln to pH=10 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 6 hours at 100 ℃; This yeast soln of re-adjustment is to pH=6.2; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out spraying drying, obtains low nucleic acid yeast sample 5
#
Embodiment 6
Get the beer yeast slurry behind debitterize that Angel Yeast Co.,Ltd produces, add deionized water, be mixed with mass percent concentration and be 9% solution; Regulate this yeast soln to pH=7 with sulfuric acid and sodium hydroxide; Then, this yeast soln is incubated 5 hours at 85 ℃; This yeast soln of re-adjustment is to pH=5.8; Separate with stacked separating machine, collect the heavy phase of separating, add water then in the gained heavy phase, amount of water is identical with gained heavy phase volume, stirs and separates with separating machine after 30 minutes again, and the gained heavy phase is carried out spraying drying, obtains low nucleic acid yeast sample 6
#
Each composition quality percentage composition is as shown in the table in each embodiment gained sample:
Nucleic acid (in RNA) | Protein | Calcium (ppm) | Iron (ppm) | Zinc (ppm) | |
Sample 1 # | 1.0% | 45% | 1500 | 100 | 200 |
Sample 2 # | 0.41% | 53% | 1607 | 300 | 390 |
Sample 3 # | 0.22% | 51% | 1721 | 500 | 400 |
Sample 4 # | 0.05% | 54% | 3827 | 700 | 930 |
Sample 5 # | 0.6% | 55% | 5000 | 1000 | 1000 |
Sample 6 # | 0.8% | 49% | 100 | 30 | 30 |
Two, experimentation on animals
With the low nucleic acid yeast product sample 1 among the preparation embodiment
#, 2
#, 3
#, 4
#, 5
#, 6
#, the Angel face carries out experimentation on animals simultaneously with yeast, Angel yeast saccharomyces cerevisiae, Angel cereuisiae fermentum, beer yeast slurry, nursing experiment through 30 days is found, low nucleic acid yeast group is not remarkable to uric acid influence in the experiment rat serum, and common yeast to the influence of rat serum uric acid level significantly, uric acid in the rat serum that can significantly raise.
When low nucleic acid yeast of the present invention and common yeast carry out experimentation on animals, variation such as the following table of uric acid content in the interior serum of big white mouse body:
Uric acid in the initial serum | After 30 days in the serum |
Content (μ mol/L) | Uric acid content | |
Blank group | 90.28±2.6 | 91.98±2.6 |
Sample 1# | 90.21±2.0 | 92.21±2.4 |
Sample 2# | 90.32±2.2 | 91.87±2.1 |
Sample 3# | 90.40±2.4 | 92.43±2.7 |
Sample 4# | 90.36±2.3 | 91.34±2.3 |
Sample 5# | 90.38±2.5 | 92.18±2.8 |
Sample 6# | 90.41±2.7 | 92.24±2.4 |
The face yeast | 90.29±2.2 | 105.70±3.8 |
Yeast saccharomyces cerevisiae | 90.52±2.1 | 106.27±4.5 |
Cereuisiae fermentum | 90.64±2.8 | 118.67±4.9 |
Beer yeast slurry | 90.68±2.9 | 121.42±5.0 |
Therefore, compare the Angel face according to the low nucleic acid yeast group of products of the inventive method production and with yeast, Angel yeast saccharomyces cerevisiae, Angel cereuisiae fermentum, beer yeast slurry group the influence of high lithemia patient or patient with gout is much smaller, therefore safer.
The present invention is through trial-production and produce the existing existing qualified product that meet company standard.
Three, preparation diet products
Embodiment 1
Formula for a product (kg/ton)
Low nucleic acid yeast 90.049
CALCIUM PYRUVIC 4.0
L-carnitine 4.0
VITMAIN B1 0.1
Wei ShengsuB2 0.1
Vitamin B6 0.1
Vitamin B12 0.0005
Folic acid 0.1
Nicotinic acid 0.5
Pantothenic acid 0.5
Vitamin A 0.05
Vitamins C 0.5
Vitamins D 0.0005
Production technique:
Raw material in the above prescription by the prescription weighing, is then dropped into together in the three-dimensional mixer and mixed 40 minutes, be packaged into the 300g/ bottle with the powder packets installation then, wrap into the storehouse then greatly.
Claims (8)
1. a low nucleic acid yeast is characterized in that, protein content is 45~55%, and nucleic acid content is 0.05~1.0%, and calcium contents is 100~5000ppm, and iron level is 30~1000ppm, and zinc content is 30~1000ppm; Described yeast is yeast saccharomyces cerevisiae.
2. the preparation method of a low nucleic acid yeast as claimed in claim 1 comprises:
1) the yeast water is mixed with the solution that mass percent concentration is 5-10%;
2) regulating step 1) the gained yeast soln is to pH=6.0-10.0;
3) with step 2) the gained yeast soln 60-100 ℃ the insulation 1-6 hour;
4) re-adjustment step 3) gained yeast soln is to pH=5.0~7.0;
5) separate washing, drying step 4) the gained yeast soln, obtain low nucleic acid yeast product.
3. the preparation method of a low nucleic acid yeast as claimed in claim 2 is characterized in that, to be mixed with mass percent concentration be 7~10% to water in the described step 1).
4. the preparation method of a low nucleic acid yeast as claimed in claim 2 is characterized in that, to be mixed with mass percent concentration be 8% to water in the described step 1).
5. the preparation method of a low nucleic acid yeast as claimed in claim 2 is characterized in that, described step 2) in regulating step 1) the gained yeast soln is to pH=7.0-9.0,
6. the preparation method of a low nucleic acid yeast as claimed in claim 5 is characterized in that, described step 2) in regulating step 1) the gained yeast soln is to pH=8.5.
7. the preparation method of a low nucleic acid yeast as claimed in claim 2 is characterized in that, described step 3) is with step 2) the gained yeast soln 80-100 ℃ the insulation 2-4 hour.
8. the preparation method of a low nucleic acid yeast as claimed in claim 7 is characterized in that, described step 3) is with step 2) the gained yeast soln 95 ℃ the insulation 3 hours.
Priority Applications (3)
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CN2009102158911A CN102115717B (en) | 2009-12-31 | 2009-12-31 | Low nucleic acid yeast product, preparation method thereof and weight-reducing product comprising same |
PCT/CN2010/078570 WO2011079652A1 (en) | 2009-12-31 | 2010-11-09 | Yeast product with low nucleic acid content, method preparing the same and diet product containing said yeast product with low nucleic acid content |
KR1020127003420A KR20120057613A (en) | 2009-12-31 | 2010-11-09 | Yeast product with low nucleic acid content, method preparing the same and diet product containing said yeast product with low nucleic acid content |
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MX352973B (en) * | 2011-09-22 | 2017-12-15 | Danisco Us Inc | Endogenous dnase activity to reduce dna content. |
CN103082081B (en) * | 2011-11-01 | 2015-11-25 | 安琪酵母股份有限公司 | A kind of Yeast protein and method for making thereof, with this albumen be raw material food and method for making thereof |
CN103126968B (en) * | 2011-11-30 | 2014-09-03 | 安琪酵母股份有限公司 | Zinc-rich yeast refined extract and preparation method thereof |
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CN1606932A (en) * | 2003-10-14 | 2005-04-20 | 兰敬墨 | Preparation of health nutritious beverage for fat reduction using yeast |
CN101322554B (en) * | 2008-07-18 | 2012-07-04 | 哈尔滨医科大学 | Health products for reducing fat |
CN101444289A (en) * | 2008-11-24 | 2009-06-03 | 江苏省江大绿康生物工程技术研究有限公司 | Technology for simultaneously preparing cooking wine and slimming food |
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US4564523A (en) * | 1983-07-29 | 1986-01-14 | Phillips Petroleum Company | Functional protein products |
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