CN102115716B - Sporidiobolus pararoseus bacterial strain and application thereof - Google Patents
Sporidiobolus pararoseus bacterial strain and application thereof Download PDFInfo
- Publication number
- CN102115716B CN102115716B CN 201010576329 CN201010576329A CN102115716B CN 102115716 B CN102115716 B CN 102115716B CN 201010576329 CN201010576329 CN 201010576329 CN 201010576329 A CN201010576329 A CN 201010576329A CN 102115716 B CN102115716 B CN 102115716B
- Authority
- CN
- China
- Prior art keywords
- carotene
- bacterial strain
- carotenoid
- strain
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to a sporidiobolus pararoseus bacterial strain and application thereof. The bacterial strain is a sporidiobolus pararoseus bacterial strain JD-2 CCTCC M 2010326. A process of producing carotene by using the sporidiobolus pararoseus bacterial strain JD-2 comprises the following steps of: (1) activating and culturing the sporidiobolus pararoseus bacterial strain; (2) fermenting and culturing the activated strain obtained in the step (1) at the pH value between 5 and 9; and (3) extracting the bacterial liquid obtained in the step (1) and/or the step (2) to obtain a carotene extract. The yield of the carotene produced by using the sporidiobolus pararoseus bacterial strain is generally up to 500-700 mug/g, process conditions such as temperature, pH value and the like are wide, the needed carotene is selectively gathered by controlling different fermentation conditions, and the produced carotene belongs to a pure natural product, does not contain any artificial color or other chemical additives, and can be widely applied to the fields of food industry, cosmetic industry, pharmaceutical industry and the like.
Description
Technical field
The present invention relates to a Yeasts and the application thereof in microorganism and microbial fermentation field, relate in particular to a kind of lock and throw yeast strain and the application in the carotenoid production technique thereof.
Background technology
Carotenoid is the fat-soluble yellow-red pigment that a class extensively exists in plant, animal and microorganism, and structure is C
40The polyisoprene structure, wherein the difference of conjugated double bond can cause the variation of color.Carotenoid mainly is divided into carotenoid and Radix Dauci Sativae alcohols (xenthophylls class), and carotenoid comprises: alpha-carotene, β-carotene, gamma carotene, Lyeopene and Torulene (Torulene); Radix Dauci Sativae alcohols (xenthophylls class) then comprises: zeaxanthin, cryptoxanthin, xenthophylls, Capsorubin, Gardenia Yellow.
β-carotene is have homovitamin A activity in all carotenoid a kind of.It effectively the various biomacromolecules in the Cell protection be not subjected to the damage of free radical, thereby keep the normal life metabolism of cell, prevent aging and canceration.The U.S. etc. have been used for β-carotene the control of VA and cancer and thin vascular disease as the medicine emphasis.As foodstuff additive, carotenoid is also regarded as category-A nutrition pigment by FAO and WTO, and is widely used as the foster enriching substance of food colorant concurrent management and the function of health food factor by western developed country.
The main source of natural beta-carotin has two kinds: a kind of is to extract from plant and salt algae; Another kind of then be that the microorganism that utilize to produce β-carotene or its precursor substance carries out fermentative production.The method of directly extracting natural beta-carotin from plant is subject to the restriction of the conditions such as material content, weather, the place of production and transportation, be difficult to a large amount of productions, utilize high salt, coastal waters short of rain to cultivate the salt algae such as countries such as the U.S., Australia and Israel and extract natural beta-carotin, also be subjected to region and technical limitation, do not meet China's national situation.For utilizing microbial technique to produce the technique of natural beta-carotin, both at home and abroad main good " blakeslea trispora " (Blakeslea) and " rhodotorula " (Rhodotorula).The technique that adopts blakeslea trispora both sexes strain fermentation to produce β-carotene has the biomass height, produces the advantages such as the β-carotene ability is strong, suitable realization industrialization, especially in recent years, by updating fermenting process, its fermentation production rate improves constantly, be in the industrialization development conceptual phase, product is put on market.But, because blakeslea trispora is zygogamy, cause its proterties more easily to fail, and have fermentation technique complex process, long, the high in cost of production shortcoming of fermentation period.But adopt the rhodotorula producing beta-carotene by fermentation to have that nutritional requirement is simply extensive, fermentation period short, mode of reproduction is simple, the nutritional condition requirement is low and the advantage such as high-density culture, but present fermentation level still not as blakeslea trispora, still fails to carry out commercial application.
Summary of the invention
One of purpose of the present invention is to propose a kind of lock and throws yeast strain, and it can be applicable to produce carotenoid, and productive rate is high, thereby overcomes deficiency of the prior art.
For achieving the above object, the present invention has adopted following technical scheme:
A kind of lock is thrown yeast strain Sporidiobolus pararoseus JD-2CCTCC M 2010326, and this bacterial strain is preserved in Chinese Typical Representative culture collection center (CCTCC) on December 3rd, 2010, and preserving number is CCTCC M 2010326.
Another object of the present invention is to use above-mentioned lock and throw yeast strain production carotenoid, its technique comprises the steps:
(1) lock is thrown yeast strain Sporidiobolus pararoseus JD-2CCTCC M 2010326 and carry out strain activation and culture;
(2) adopt step (1) gained activated spawn fermentation culture in the condition of pH 5-9;
(3) step (1) and/or step (2) gained bacterium liquid are extracted operation, obtain Carotenoids Extracts.
Preferably, in step (1) or the step (2), adopt glucose as optimum carbon source, its optimal concentration is 40g/L.
Preferably, in step (1) or the step (2), adopt corn steep liquor as the optimum nitrogen source of enrichment β-carotene, its optimal concentration 20g/L; And adopt yeast extract paste as the optimum nitrogen source of enrichment Torulene, its optimal concentration 20g/L.
Preferably, step (1) is specially: bacterial strain is received on the activated inclined plane substratum from the preservation inclined-plane, cultivating under pH 5-9,25-28 ℃ the condition more than the 30-48h, access thereafter in the liquid seed culture medium, be under 25-28 ℃ the condition more than the aerlbic culture 16h in pH5-9, temperature.
Preferably, described activated inclined plane substratum comprises following component: glucose 20g/L, peptone 1g/L and yeast extract paste 1g/L, its pH5-9;
Described liquid seed culture medium comprises such as component: glucose 40g/L, corn steep liquor or yeast extract paste 20g/L, (NH4)
2SO
45g/L, KH
2PO
41g/L and MgSO
47H
2O 0.5g/L, its pH5-9.
Preferably, in the fermentation culture process, early stage, the thalli growth stage oxygen dissolving was controlled at the 20-30% saturation ratio, and the later stage dissolved oxygen is controlled at 0-5% and is controlled at 30-40% with the enrichment Torulene with enrichment β-carotene or dissolved oxygen.
Preferably, the process of step (3) is specially: step (1) and/or (2) gained bacterium liquid are carried out centrifugal treating acquisition thalline, then adopt height to force down the broken somatic cells of acid system, then adding volume ratio is 4: 1 alcohol and the mixed solution of normal hexane, more than the room temperature vibration lixiviate 0.5h, obtain the carotenoid crude extract.
Further, in aforementioned fermentation culture process, optimal culture condition is: 28 ℃ of temperature, pH6.0.
Compared with prior art, beneficial effect of the present invention is: the productive rate that this lock is thrown yeast strain Sporidioboluspararoseus JD-2 production carotenoid is higher, generally can reach 500-700 μ g/g, the scope such as processing condition such as temperature, pH is wide, and can be by the different required carotenoid of fermentation condition selective enrichment of control, and the carotenoid of producing belongs to all-natural product, do not contain artificial color or other chemical additives, can be widely used in each field such as foodstuffs industry, cosmetic industry, pharmaceutical industry.
Description of drawings
Fig. 1 is that the present invention locks the electromicroscopic photograph of throwing yeast JD-2;
Fig. 2 A is the high-efficient liquid phase chromatogram that application lock is thrown yeast carotenoid that JD-2 produces (enrichment β-carotene) in the preferred embodiment of the present invention;
Fig. 2 B is the high-efficient liquid phase chromatogram that application lock is thrown yeast carotenoid that JD-2 produces (enrichment Torulene) in the preferred embodiment of the present invention;
Among Fig. 2 A and Fig. 2 B, peak A
19, B
18Represent torularhodin (564); Peak A
20, B
23Be Torulene (534); A
24, B
24Be β-carotene (536).
Embodiment
For existing carotenoid production technique, especially with the deficiency of yeast production carotenoid technique, this case contriver proposes a kind of lock that never is seen in before this report and throws yeast Sporidiobolus pararoseusJD-2, and it is applied to the production technique of carotenoid.
This lock is thrown yeast strain Sporidiobolus pararoseus JD-2 and is obtained through screening, separation from Wuxi curry favour mountain forest ground soil.
This bacterial strain preferred growth is grown under 4～40 ℃ of culture temperature and pH4.0～9.0 scopes in the YPD media surface, and on the YPD substratum, bacterium colony is rounded, orange red, smooth surface, homogeneous, neat in edge, easily provokes.Observe under Electronic Speculum, thalline is oblong, cell size (4～5) * (2～3) μ m, monolateral sprouting.Assimilation glucose, maltose, seminose, sucrose, glycerine, semi-lactosi, wood sugar, alcohol do not assimilate lactose, lactic acid.For identifying this bacterium the chemotaxonomy that it carries out take the ITS sequential analysis as foundation is studied, similarity according to sequence compares, the result shows that throwing yeast (Sporidioboluspararoseus) CBS484 with lock reaches 100% similarity, and its called after lock is thrown yeast (Sporidioboluspararoseus) JD-2.It is as shown in table 1 below that this lock is thrown the bacteria characteristic of yeast strain JD-2.
The physio-biochemical characteristics that table 1 lock is thrown yeast strain JD-2
Annotate: in the table, ++: well-grown ,+-: it is faint to grow,--: do not grow
Method of production as above-mentioned dissociant comprises physical mutagenesis, such as various Irradiations such as ultraviolet radiation treatment, co-60 radiation processing, ion implantation processing, laser radiation, microwave radiations; Chemomorphosis is processed, and optimizes preferably bacterial strain of production performance with conventional carotenoid generation bacterium separation screening substratum and method.
In addition, also can pass through Protocols in Molecular Biology, from original strain or dissociant, obtain the gene of synthetic carotenoid, make up genetic engineering bacterium., make up genetically engineered and produce bacterial strain as the genetic recipient bacterium such as intestinal bacteria, subtilis and yeast etc., also can be used as carotenoid producing bacterial strain of the present invention.
Can adopt following method that lock of the present invention is thrown yeast strain JD-2 and preserve, activate and screen, and utilize it to obtain carotenoid by techniques such as shake flask fermentations:
The bacterial classification that lock of the present invention is thrown yeast strain JD-2 can adopt the conventional inclined-plane method that goes down to posterity to preserve, this method is lock to be thrown yeast strain JD-2 be inoculated in any chamfered surface that is fit to Yeast Growth, preferred YPD substratum (the glucose 20g/L that uses, peptone 10g/L, yeast extract paste 10g/L), its culture condition is pH 5-9, temperature 25-28 ℃.
The bacterial classification of long-term preservation activates as follows in use, screens:
The bacterial classification inoculation of long-term preservation is suitable for the substratum of Yeast Growth in YPD substratum or other, such as the wort solid medium etc., at pH 5-9, under the temperature 25-28 ℃ of condition, carries out fermentation culture behind the solid slant culture 48h, obtain carotenoid.
In the fermentation culture process, also the slant strains direct inoculation can be carried out fermentation culture in fermention medium.
This case the contriver find after deliberation, and in the fermentation culture process, carbon source glucose commonly used is conducive to the generation of carotenoid most, and optimal concentration is 40g/L; Corn steep liquor is the optimum nitrogen source of enrichment β-carotene at high proportion, its optimal concentration 20g/L; Yeast extract paste is the optimum nitrogen source of enrichment Torulene at high proportion, its optimal concentration 20g/L; And in the fermentation culture process, front 24h thalli growth stage oxygen dissolving is controlled at the 20-30% saturation ratio, and the later stage is hanged down dissolved oxygen (0-5%) enrichment β-carotene, high dissolved oxygen (30-40%) enrichment Torulene.
Usually, can select following condition to cultivate.Be culture temperature 25-28 ℃, be preferably 28 ℃; More than the incubation time 30h, preferred 30-48h certainly, also can be not limited to this, and as long as when the output of carotenoid of the present invention reaches the highest, finish fermentation culture; The pH of substratum can in the production range of pH5-9, especially be more suitable for the fermentation of carotenoid of the present invention at pH6.Through above-mentioned fermentation culture, can produce carotenoid.
The present invention provides simultaneously and utilizes aforementioned lock to throw the method that yeast strain JD-2 produces carotenoid, and it comprises the steps: 1) strain culturing: lock is thrown yeast JD-2 activation, the centrifugal thalline that obtains of fermentation culture certain hour; 2) adopt height to force down the broken somatic cells of acid system, then adding volume ratio is 4: 1 alcohol and the mixed solution of normal hexane, more than the room temperature vibration lixiviate 0.5h, obtains the carotenoid crude extract.
Below in conjunction with accompanying drawing and some preferred embodiments technical scheme of the present invention is elaborated.
Embodiment 1 lock is thrown the strain culturing of yeast (Sporidiobolus pararoseus) JD-2 and determining of enrichment β-carotene zymotechnique.
Select substratum as follows:
(1) slant activation substratum (g/L): glucose 20, peptone 1, yeast extract paste 1, pH 6.0.
(2) liquid seed culture medium of enrichment β-carotene (g/L): glucose 40, corn steep liquor 20, (NH4)
2SO
45, KH
2PO
41, MgSO
47H
2O 0.5, and pH 6.0, the 100mL/500mL triangular flask.
(3) liquid fermentation medium of enrichment β-carotene (g/L): glucose 40, corn steep liquor 20, (NH4)
28O
45, KH
2PO
41, MgSO
47H
2O 0.5, and pH 6.0.
Fermentation culture method:
(1) slant activation is cultivated: bacterial strain is received on the activated inclined plane substratum from the preservation inclined-plane, cultivated 48h for 28 ℃.
Liquid seeds is cultivated: from activated inclined plane substratum access liquid seed culture medium, 16h is cultivated in 28 ℃ of vibrations (100r/min, reciprocating type shaking table, amplitude 8cm) with bacterial strain.
(2) liquid fermentation and culture: the liquid seeds access 7L fermentor tank after the step of learning from else's experience (1) is processed, inoculum size 10% (V/V), and adding liquid fermentation medium, liquid amount 3.5L, cultivate 72h for 28 ℃, front 24h thalli growth stage oxygen dissolving is controlled at the 20-30% saturation ratio, and the later stage is hanged down dissolved oxygen 0-5%, enrichment β-carotene.
The mensuration of cellular biomass: with step (1), (2) gained bacterium liquid centrifugal treating (4000rpm, 10min), abandon supernatant liquor, recentrifuge after the precipitation washing, gained precipitation (yeast slurry) causes constant weight, weighs in 60 ℃ of bakings, passes through the thalline broken wall again, obtains Carotenoids Extracts after the steps such as organic solvent extraction, its total carotinoid output is 500-700 μ g/g, β-carotene output 350-400 μ g/g.
Embodiment 2 lock is thrown the determining of zymotechnique of the strain culturing of yeast (Sporidiobolus pararoseus) JD-2 and enrichment Torulene.
Select substratum as follows:
(1) slant activation substratum (g/L): glucose 20, peptone 1, yeast extract paste 1, pH6.0.
(2) liquid seed culture medium of enrichment Torulene (g/L): glucose 40, yeast extract paste 20, (NH4)
2SO
45, KH
2PO
41, MgSO
47H
2O 0.5, pH6.0,100mL/500mL triangular flask.
(3) liquid fermentation medium of enrichment Torulene (g/L): glucose 40, yeast extract paste 20, (NH4)
2SO
45, KH
2PO
41, MgSO
47H
2O 0.5, pH6.0.
Fermentation culture method:
(1) slant activation is cultivated: bacterial strain is received on the activated inclined plane substratum from the preservation inclined-plane, cultivated 48h for 28 ℃.
Liquid seeds is cultivated: from activated inclined plane substratum access liquid seed culture medium, 16h is cultivated in 28 ℃ of vibrations (100r/min, reciprocating type shaking table, amplitude 8cm) with bacterial strain.
(2) liquid fermentation and culture: the liquid seeds access 7L fermentor tank after the step of learning from else's experience (1) is processed, inoculum size 10% (V/V), and adding liquid fermentation medium, liquid amount 3.5L, cultivate 72h for 28 ℃, front 24h thalli growth stage oxygen dissolving is controlled at the 20-30% saturation ratio, high dissolved oxygen 30-40% of later stage, enrichment Torulene.
The mensuration of cellular biomass: with step (1), (2) gained bacterium liquid centrifugal treating (4000r/min, 10min), abandon supernatant liquor, recentrifuge after the precipitation washing, gained precipitation (yeast slurry) causes constant weight, weighs in 60 ℃ of bakings, passes through the thalline broken wall again, obtains Carotenoids Extracts after the steps such as organic solvent extraction, its total carotinoid output is 500-700 μ g/g, Torulene output 450-600 μ g/g.
The extraction of embodiment 3 carotenoid
The detailed process that makes Carotenoids Extracts in the previous embodiment 1 and 2 can be: will contain the centrifugal 10min of bacterium liquid 4000r/min that lock is thrown yeast (Sporidiobolus pararoseus) JD-2, obtain thalline, with deionized water wash once, the hydrochloric acid of the centrifugal rear adding 0.6mol/L that anhydrates, in Autoclave, heat up and cause 115 ℃, rapid bleed, centrifugal removal hydrochloric acid, throw out with deionized water wash once, centrifugal anhydrate minute again, adding volume ratio is 4: 1 alcohol and the mixture of normal hexane, room temperature vibration lixiviate 30min, again centrifugal treating (4000r/min, 10min), get supernatant liquor at 40 ℃, concentrate under the condition of vacuum tightness 0.1Mpa, obtain the carotenoid crude extract.
Example 4 carotenoid compositional analysis
This experiment is carried out the composition of aforementioned carotenoid crude extract is carried out qualitative and quantitative analysis with liquid phase chromatography and mass spectroscopy.
Reagent: acetonitrile (chromatographically pure), methylene dichloride (chromatographically pure), the β-carotene standard specimen is purchased from SIGMA company, and content is greater than 98%.
HPLC condition: (1) gradient elution: front 10min is upgraded to 100% (volume ratio of acetonitrile and methylene dichloride is 1: 1) and is kept 5min by 100% acetonitrile; (2) flow velocity 1mL/min; (3) sample size 10 μ L; (4) detect wavelength 470nm; (5) column temperature is 30 ℃; (6) chromatographic column: D3.9 * 150mm Nova-Pak C18, particle diameter 5 μ m.
Mass spectrum condition: elution requirement the same (Waters Maldi Synapt Q-TOF)
Consult Fig. 2 A and Fig. 2 B, LC-MS result shows that the main carotenoid that lock is thrown in the yeast is: β-carotene, Torulene and torularhodin.Because only have β-carotene that commercial standard specimen is arranged, so the amount of total carotinoid amount and Torulene obtains take the absorption peak area of β-carotene standard specimen as basis conversion.Total carotinoid output 500-700 μ g/g in the β-carotene enrichment medium, β-carotene output 350-400 μ g/g; Total carotinoid 500-700 μ g/g in the Torulene enrichment medium, the output 450-600 μ g/g of Torulene.
Above-mentioned description by the implementation example is intended to easy to understand technical scheme feature of the present invention, and protection scope of the present invention is not constituted any limitation.All employing equivalents or equivalence are replaced and the technical scheme of formation, all drop within the rights protection scope of the present invention.
Claims (8)
1. a lock is thrown yeast strain (Sporidiobolus pararoseus) JD-2, it is characterized in that the preserving number of described bacterial strain is CCTCC NO:M 2010326.
2. lock is thrown the application of yeast strain (Sporidiobolus pararoseus) JD-2 in the carotenoid production technique as claimed in claim 1.
3. the production method of a Carotenoids is characterized in that, described method comprises the steps:
(1) the described lock of claim 1 is thrown yeast strain Sporidiobolus pararoseus JD-2 and carry out strain activation and culture;
(2) adopt step (1) gained activated spawn to cultivate at the condition bottom fermentation of pH 5-9;
(3) step (1) and/or step (2) gained bacterium liquid are extracted operation, obtain Carotenoids Extracts.
4. the production method of carotenoid according to claim 3 is characterized in that, in step (1) or the step (2), adopts glucose as optimum carbon source, and its optimal concentration is 40g/L.
5. the production method of carotenoid according to claim 3 is characterized in that, in step (1) or the step (2), adopts corn steep liquor as the optimum nitrogen source of enrichment β-carotene, its optimal concentration 20g/L; And adopt yeast extract paste as the optimum nitrogen source of enrichment Torulene, its optimal concentration 20g/L.
6. the production method of carotenoid according to claim 3, it is characterized in that, step (1) is specially: bacterial strain is received on the activated inclined plane substratum from the preservation inclined-plane, under pH 5-9,25-28 ℃ condition, cultivate 30-48h, thereafter accessing in the liquid seed culture medium, is under 25-28 ℃ the condition more than the aerlbic culture 16h in pH 5-9, temperature.
7. the production method of carotenoid according to claim 6 is characterized in that:
Described activated inclined plane substratum comprises following component: glucose 20g/L, peptone 1g/L and yeast extract paste 1g/L, its pH5-9;
Described liquid seed culture medium comprises following component: glucose 40g/L, corn steep liquor or yeast extract paste 20g/L, (NH4)
2SO
45g/L, KH
2PO
41g/L and MgSO
47H
2O 0.5g/L, its pH5-9.
8. the production method of carotenoid according to claim 3, it is characterized in that: in the fermentation culture process, optimal culture condition is: 28 ℃ of temperature, pH6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010576329 CN102115716B (en) | 2010-12-07 | 2010-12-07 | Sporidiobolus pararoseus bacterial strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010576329 CN102115716B (en) | 2010-12-07 | 2010-12-07 | Sporidiobolus pararoseus bacterial strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102115716A CN102115716A (en) | 2011-07-06 |
| CN102115716B true CN102115716B (en) | 2013-03-13 |
Family
ID=44214642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010576329 Active CN102115716B (en) | 2010-12-07 | 2010-12-07 | Sporidiobolus pararoseus bacterial strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102115716B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719367B (en) * | 2012-07-10 | 2013-06-12 | 湖南农业大学 | Catalytic synthesis method of L-theanine by using microorganism-produced gamma-glutamyl amino carboxamide synthase |
| CN103087930B (en) * | 2012-08-29 | 2015-03-25 | 漯河医学高等专科学校 | Sporidiobolus pararoseus strain and application thereof in foods and feeds |
| CN109554410B (en) * | 2018-12-14 | 2020-06-26 | 江南大学 | Feeding method for high-density fermentation of throw-locked yeast |
| CN109548984A (en) * | 2018-12-19 | 2019-04-02 | 江南大学 | A method of oil crops are processed using rhodotorula |
| CN110396478B (en) * | 2019-04-26 | 2022-06-24 | 盐城工学院 | Sporobolomyces and method for optimizing and extracting carotenoid by using response surface method |
| CN110257266B (en) * | 2019-05-14 | 2020-03-06 | 华南农业大学 | Near-rose color-locked yeast strain and application thereof in production of lactase |
| CN110241032B (en) * | 2019-08-09 | 2021-11-16 | 浙江树人学院（浙江树人大学） | Sporobolomyces rosenbergii and application thereof |
| CN110734867B (en) * | 2019-11-29 | 2021-04-20 | 自然资源部第三海洋研究所 | Deep-sea-derived yeast Q7-7 and application thereof |
| CN111793569A (en) * | 2020-08-05 | 2020-10-20 | 盐城工学院 | High-yield fermentation method and application of carotenoid high-yield spore-casting yeast strain |
| CN113373197B (en) * | 2021-07-21 | 2022-10-11 | 江南大学 | Application of Spodoptera frugiperda in production of carotenoid and exopolysaccharide |
| CN113355250B (en) * | 2021-07-21 | 2022-08-23 | 江南大学 | Lipid yeast and culture method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008000A (en) * | 2006-01-25 | 2007-08-01 | 新疆大学 | Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method |
-
2010
- 2010-12-07 CN CN 201010576329 patent/CN102115716B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101008000A (en) * | 2006-01-25 | 2007-08-01 | 新疆大学 | Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method |
Non-Patent Citations (1)
| Title |
|---|
| 范永仙等.微生物生产类胡萝卜素的研究进展.《食品与发酵工业》.2003,第29卷(第7期),全文. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102115716A (en) | 2011-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102115716B (en) | Sporidiobolus pararoseus bacterial strain and application thereof | |
| CN103782801B (en) | White mushroom liquid spawn and preparation method thereof | |
| CN104893983B (en) | Liquid state fermentation low citrinin, the preparation method of High color values monascorubin and product | |
| CN101831471B (en) | Method for producing polysaccharides by fermenting yellow serofluid with edible and medicinal fungi | |
| CN101988036B (en) | Aureobasidium pullulans strain as well as preparation method and application thereof in producing pigment-free pullulan | |
| CN106434373A (en) | High-density fermentation medium formula of sparassis crispa and pharmaceutical grade glucan preparation method of high-density fermentation medium formula | |
| CN101235353B (en) | Monascus mutant and method for preparing flavochrome by fermenting the same | |
| CN101168728A (en) | Shiraia strain for perylene producing quinone compound | |
| CN102630490A (en) | Artificial cultivation method for cordyceps militaris mycelium for increasing cordycepin content | |
| CN106010980B (en) | A kind of endogenetic fungus Brazil class shell roundlet spore bacterial strain and its application | |
| CN103484290B (en) | Brewing method of low-alcohol-content dry blueberry wine | |
| CN104372061A (en) | Preparation method of zinc-enriched liquid fermentation product of phellinus linteus | |
| CN110093281B (en) | Phellinus igniarius liquid submerged fermentation culture process | |
| CN105969702B (en) | Serratia marcescens RZ 21-C6 and its application | |
| CN109810909B (en) | Phaffia rhodozyma strain for high yield of lycopene and production method of lycopene | |
| CN100447233C (en) | Medium temperature type astaxanthin producing bacterial strain and its culture process | |
| CN104845893A (en) | Rhodotorula mucilaginosa and application thereof to fermentation production of malus micromalus astaxanthin | |
| CN102766663B (en) | Preparation method of active polysaccharides from phellinus linteus | |
| CN108841889B (en) | Method for producing griseofulvin serving as major component of tranexamycin by microbial fermentation | |
| CN106480155B (en) | Method suitable for promoting haematococcus pluvialis to produce astaxanthin under high-temperature condition | |
| CN103374537A (en) | Method for preparing enduracidin and strain produced thereby | |
| CN100543128C (en) | A kind of red torula of viscosity, β-Hu Luobusu and production method thereof of producing β-Hu Luobusu | |
| CN108796027B (en) | Method for producing carotenoid | |
| CN106718016A (en) | A kind of earthing matrix for improving agaricus bisporus fruit-body formation amount and preparation method thereof | |
| CN105087737A (en) | Method for preparing astaxanthin by fermenting Jerusalem artichoke and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |