CN102103145B - Colloidal gold test strip for double-amplification system and preparation method thereof - Google Patents
Colloidal gold test strip for double-amplification system and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of medical clinical immediate tests, and relates to a colloidal gold test strip for a double-amplification system and a preparation method thereof. The colloidal gold test strip for the double-amplification system comprises a test strip bottom lining, and a sample pad, a polyester film coated with a gold-labeled antibody, a coating film and absorbent paper which are lapped with and adhered to the test strip bottom lining in turn, wherein the middle part of the coating film is parallelly provided with a detection line coated with streptavidin and an F(ab)2 fragment of a monoclonal antibody of an antigen to be detected, and a control line coated with a rabbit anti-rat immunoglobulin G (IgG) antibody; the F(ab)2 fragment of the monoclonal antibody of the antigen to be detected and biotin are coated in the sample pad; and the gold-labeled antibody is colloidal gold labeled protein A and the monoclonal antibody of the antigen to be detected. The test strip has the advantages of high specificity, sensitivity and detection speed, has low cost, is easy to operate, can be widely applied to the detection of the antigen to be detected, and lays a foundation for the timely discovery and prediction and effective detection of diseases, and an instrument and equipment are not required to be used.
Description
Technical field
The invention belongs to clinical medicine real-time test field, relate to colloidal gold strip of a kind of pair of amplification system and preparation method thereof, specifically relate to a kind of colloidal gold strip that detects cardiac muscle troponin I and preparation method thereof.
Background technology
At present, the whole world has 1,700 ten thousand people to die from angiocardiopathy every year, the acute myocardial infarction AMI of dying from over half is wherein arranged, China's acute myocardial infarction rate is about 0.02%～0.06%, the number of dying from acute myocardial infarction AMI every year has surpassed 1,000,000, and " rejuvenation " trend appears in the morbidity crowd, and cardiovascular and cerebrovascular disease has now become China's population main causes of death, and the incidence of disease of the ischemia myocardial damage that is particularly caused by myocardial infarction increases year by year.Therefore diagnose with active treatment most important to myocardial damage in early days, the expert points out, catch pectoralgia show effect rear 6 hours " prime time " be the key for the treatment of patient life, cTnI is because of its outstanding sensitivity and specificity, be used as at present " goldstandard " of diagnosis of myocardial damage, both at home and abroad it be widely used in the diagnosis of acute myocardial infarction AMI, judgement, the layering of unstable angina pectoris risk factor and the prognosis judgement that thrombolysis leads to again, the clinical monitoring that acute myocarditis continues myocardial damage etc.
A lot of to the detection method of cardiac muscle troponin I, and immune-gold labeled method is to grow up after 90, quick because of it, easy and simple to handle, stable reagent, but room temperature accumulating, the characteristics that are difficult for polluting are widely used.It is immune affine technology, marking technology, the combination of immune labeled and chromatographic technique.With coated antibody, the colloidal gold labeled monoclonal antibody immobilization is combined with sample sorbing material etc., prepares the immunochromatography diagnostic test, only sample solution need to be dripped on test strips during use, just can judged result in 10 minutes.
Because there is the problem of sensitivity in collaurum, also there is not further to improve at present the method for collaurum sensitivity.Be directed to this technical background, we are in existing technical two amplification systems of having developed.
Summary of the invention:
The objective of the invention is the above-mentioned deficiency for prior art, sensitivity and specificity problem that the colloidal gold strip that a kind of pair of amplification system be provided exists to solve prior art.
Another object of the present invention provides the preparation method of this test strips.
Purpose of the present invention can be achieved by the following technical solutions:
The colloidal gold strip of a kind of pair of amplification system, comprise the test strips end liner, and the sample pad, the polyester film that is coated with golden labeling antibody, coated film and the thieving paper that mutually overlap in turn stickup on the test strips end liner, a described coated film parallel coated streptavidin-determined antigen monoclonal antibody F (ab) that is provided with in middle part
2The control line of the detection line of fragment and a coated rabbit anti-mouse igg antibody has been coated with determined antigen monoclonal antibody F (ab) in the described sample pad
2Fragment-biotin, described golden labeling antibody are colloid gold label ProteinA-determined antigen monoclonal antibody.
The F (ab) of described streptavidin-determined antigen monoclonal antibody
2Fragment is the F (ab) of streptavidin and cardiac muscle troponin I monoclonal antibody
2After the fragment coupling in conjunction with product.
Described colloid gold label ProteinA-determined antigen monoclonal antibody is the ProteinA-cardiac muscle troponin I monoclonal antibody of colloid gold label.
The preparation method of above-mentioned colloidal gold strip comprises the steps:
1) preparation coated film is with the F (ab) of coated damping fluid dilution streptavidin-determined antigen monoclonal antibody
2Fragment and rabbit anti-mouse igg antibody, and two kinds of antibody after will diluting are sprayed on respectively the middle part of coated film abreast, two kinds of antibody infiltrate the control line that forms respectively determined antigen detection line and rabbit anti-mouse igg antibody behind the coated film;
2) polyester film of preparation coated with gold labeling antibody: by gold grain mark ProteinA, the Fc fragment non-specific binding of the monoclonal antibody by ProteinA and determined antigen is combined in the monoclonal antibody of determined antigen on the gold grain indirectly;
3) preparation determined antigen monoclonal antibody F (ab)
2The sample pad that fragment-biotin is coated;
4) test strips assembling.
The F (ab) of described streptavidin-determined antigen monoclonal antibody
2Fragment is the F (ab) of streptavidin and cardiac muscle troponin I monoclonal antibody
2After the fragment coupling in conjunction with product.
Step 2) described golden labeling antibody is by colloid gold particle mark ProteinA, by the Fc section non-specific binding of ProteinA and cardiac muscle troponin I monoclonal antibody, with indirectly being combined on the colloid gold particle that the cardiac muscle troponin I monoclonal antibody is amplified.
Beneficial effect and principle thereof: colloidal gold strip of the present invention utilizes the colloidal gold immunochromatographimethod technology, when determined antigen is flowed through sample pad, by the F (ab) of the determined antigen monoclonal antibody in the sample pad
2F in fragment-biotin (ab)
2Fragment is caught, by F (ab)
2The determined antigen that fragment is caught is flowed through on the polyester film, and the golden labeling antibody that another site of determined antigen is applied on the polyester film is caught, and forms the compound of antibody-Ag-Ab.Because the Fc section of the monoclonal antibody of ProteinA and determined antigen has strong binding ability, the present invention utilizes first colloid gold label ProteinA, thereby and then be combined the monoclonal antibody that makes determined antigen with the Fc of the monoclonal antibody of determined antigen section by ProteinA and indirectly amplify and be combined on the gold grain, this combination can strengthen the adhesion of the monoclonal antibody of collaurum and determined antigen, overcome in the conventional method collaurum and determined antigen monoclonal antibody binding ability that the monoclonal antibody because of the direct mark determined antigen of collaurum causes not strong, and the site that monoclonal antibody is combined with determined antigen is easily occupied the defective that causes specificity to descend by collaurum.The improvement of the present invention's gold labeling antibody can improve sensitivity on the one hand greatly, can improve specificity on the other hand, and this is to amplify for the first time.Antibody-antigen-Jin labeling antibody compound chromatography upwards under capillary effect, biotin can be combined at the streptavidin in detection line 6 subsequently, because 4 subunits of streptavidin can in conjunction with the biotin of 4 molecules, therefore form for the second time and amplify.Because streptavidin is difficult for being fixed on the cellulose nitrate coated film, and antibody fragment can be easy to be fixed on the coated film.So F (ab) of the determined antigen monoclonal antibody in the detection line 6
2Fragment plays streptavidin is fixed on effect on the coated film indirectly.Unnecessary golden labeling antibody continues chromatography to the control line position, because mouse source myocardium myo calcium monoclonal antibody and rabbit anti-mouse igg antibody generation immune response, thereby control line is developed the color because of the bridging collaurum, no matter therefore whether contain determined antigen in the sample, as long as test strips can normally be used, control line all can develop the color.Twice amplification can improve sensitivity and specificity greatly, for detection and the prediction of determined antigen provides more accurately result.This test strips has high specificity, and is highly sensitive, the advantage that detection speed is fast, and need not use instrument and equipment, and with low cost, simple to operate, can be widely used in the detection of determined antigen, in time finding and the prediction disease, effectively detect and lay a good foundation.
Description of drawings
The cross-sectional configuration schematic diagram of Fig. 1 colloidal gold strip of the present invention;
Wherein: 1. end liner; 2. sample pad; 3. polyester film; 4. coated film; 5. thieving paper; 6. detection line; 7. control line.
Embodiment
The present invention is further detailed explanation below in conjunction with the drawings and specific embodiments.
Embodiment 1
As shown in Figure 1, the colloidal gold strip of a kind of pair of amplification system, comprise test strips end liner 1, and the sample pad 2, the polyester film 3 that is coated with golden labeling antibody, coated film 4 and the thieving paper 5 that mutually overlap in turn stickup on the test strips end liner 1, be coated with detection line 6 and control line 7 on the coated film 4, the parallel F (ab) that is provided with a coated streptavidin-cardiac muscle troponin I monoclonal antibody 4C2 in coated film 4 middle parts
2The detection line 6 of fragment and the control line 7 of a coated rabbit anti-mouse igg antibody have been coated with the F (ab) of mouse cardiac muscle troponin I monoclonal antibody 4C2 in the sample pad 2
2Fragment-biotin, golden labeling antibody are by gold grain mark ProteinA, by the Fc section non-specific binding of ProteinA and cardiac muscle troponin I monoclonal antibody M155, with indirectly being combined on the gold grain that cardiac muscle troponin I monoclonal antibody M155 amplifies.Above streptavidin, the 4C2 monoclonal antibody, the M155 monoclonal antibody, rabbit anti-mouse igg antibody is all available from Fitzgerald company.
The concrete preparation method of colloidal gold strip is as follows:
The preparation of coated film:
The preparation of coated damping fluid: with 0.025M, the PBS of pH7.4 uses the 0.22u membrane filtration, place 4 ℃ for subsequent use, the term of validity 7 days.
The preparation of confining liquid: will contain 1%BSA, 1% sucrose, 0.025M, the PBS of pH7.5 uses the 0.22U membrane filtration, place 4 ℃ for subsequent use, the term of validity is 3 days.
The F (ab) of cardiac muscle troponin I monoclonal antibody 4C2
2The preparation of fragment: monoclonal antibody 4C2 crosses the SPA-SepharoseCL-4B gel chromatography column and removes the Fc fragment through tryptic digestion, collects F (ab)
2Fragment.
Streptavidin-F (ab)
2The preparation of fragment: with the F (ab) of streptavidin and monoclonal antibody 4C2
2Then fragment is utilized affinity column to carry out purifying and is obtained by the glutaraldehyde chemical coupling.
Streptavidin-F (ab)
2The preparation of fragment detection line 6: with antibody F (ab)
2Fragment is pressed the concentration of 4mg/ml, and peristaltic pump is awarded liquid measure 0.4ml/min, line speed 50m/20min, and 20 ℃ of forced air dryings are 12 hours in drying box.
The preparation of control line 7: rabbit anti-mouse igg antibody is pressed the concentration of 8mg/ml, peristaltic pump is awarded liquid measure 0.4ml/min, line speed 50m/20min, and 20 ℃ of forced air dryings are 12 hours in drying box.
Coated film 4 preparations: will contain the nitrocellulose filter of detection line 6 and control line 7 with above-mentioned confining liquid in 37 ℃ of sealings 60 minutes, take out rearmounted 37 ℃ of lower drying and processings two hours, envelope is for subsequent use.
The preparation of the polyester film 3 of coated with gold labeling antibody:
The preparation of aqueous solution of chloraurate: with the dissolving of the tri-distilled water of 10g gold chloride 1000ml, be made into 1% aqueous solution, place 4 ℃ for subsequent use, the term of validity 3 months.
The preparation of trisodium citrate: dissolve trisodium citrate with tri-distilled water, be made into 1% aqueous solution, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity 7 days.
The preparation of 1% wet chemical: 1g sal tartari is dissolved with tri-distilled water with 100ml, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity is 7 days.
The preparation that the gold labeling antibody is preserved liquid: with the sucrose of 15g, the Sodium azide of 20ul, in the 1%BSA dissolving of the pH=7.4 of 100ml, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity is 7 days.
The preparation of the particle of collaurum: 1% gold chloride is diluted to 0.01% with tri-distilled water, place 95 ℃ of reactions 10 minutes, add the 1ml trisodium citrate, continue reaction 15 minutes, until the colloidal gold solution color by indigo plant after purple stain is red, add the 2ml1% solution of potassium carbonate after the cooling for subsequent use.Outward appearance should be pure, and is bright, without precipitation and floating thing.
The preparation of gold labeling antibody: regulate collaurum pH value to 7.5 with 1% solution of potassium carbonate, collaurum by 20 microgram ProteinA/ml adds ProteinA, mixing afterreaction 15 minutes, with 10000rpm centrifugal 10 minutes, remove supernatant, precipitation is sealed washed twice with 1%BSA, remove for the last time supernatant, to precipitate the BSA dissolving with 1/10th 1%pH=7.4, then press the monoclonal antibody of 5ug antibody/ml collaurum adding cardiac muscle troponin I, mixing reaction 15 minutes with mark cleansing solution washed twice, is preserved the liquid dissolving with golden labeling antibody again.Place 4 ℃ for subsequent use, the term of validity is 7 days.
The collaurum that mark is good is layered on the polyester film uniformly, and discharge rate is the 0.9ul/cm*3 road, drying, and envelope, for subsequent use.
The processing of sample pad: sample pad is soaked a few hours with the 100mMPBS damping fluid, after the taking-up drying, press the F (ab) of 20ug-mouse cardiac muscle troponin I monoclonal antibody 4C2
2The F (ab) of the amount spraying mouse cardiac muscle troponin I monoclonal antibody 4C2 of fragment-biotin/every sample pad (22mm*300mm)
2Fragment-biotin.
End liner 1, sample pad 2 and thieving paper 5 are the general parts in this area.Above-mentioned sample pad 2, the polyester film 3 that is coated with golden labeling antibody, coated film 4 and thieving paper 5 are overlapped stickup in turn mutually obtain test paper plate, the test strips that at last test paper plate is cut into different in width gets final product.
Above-mentioned colloidal gold strip is in the application that detects cardiac muscle troponin I.
This test strips can be used for detecting the fluid sample of cardiac muscle troponin I, during operation, whole blood, blood plasma or serum are dripped on the sample pad 2 of this test strips, when the result who occurs is when an aubergine band respectively occurring on the detection line 6 of test strips and control line 7 positions, positive result shows and contains cardiac muscle troponin I in the sample; When only occurring an aubergine band on control line 7 positions of test strips, negative result, show contain in the sample cardiac muscle troponin I or wherein the concentration of contained cardiac muscle troponin I below the detection lower limit of this test strips; Be null result when on controlled 8 positions of test strips, the aubergine band not occurring.
The concrete preparation method of the colloidal gold strip of embodiment 2 employing conventional methods is as follows:
Cardiac muscle troponin I monoclonal antibody 4C2 and M155 (pairing antibody) are all available from Fitzgerald.
The preparation of coated film:
1) preparation of coated damping fluid: with 0.025M, the PBS of pH7.4 uses the 0.22u membrane filtration, place 4 ℃ for subsequent use, the term of validity 7 days.
2) preparation of confining liquid: will contain 1%BSA, 1% sucrose, 0.025M, the PBS of pH7.5 uses the 0.22U membrane filtration, place 4 ℃ for subsequent use, the term of validity is 3 days.
3) F of monoclonal antibody 4C2 (ab)
2The preparation of section: monoclonal antibody is crossed the SPA-SepharoseCL-4B gel chromatography column and is removed the Fc fragment through tryptic digestion, collects F (ab)
2Fragment.
4) F (ab)
2The preparation of section detection line 6: antibody press the concentration of 4mg/ml, peristaltic pump is awarded liquid measure 0.4ml/min, the speed of ruling 50m/20min, and 20 ℃ of forced air dryings are 12 hours in drying box.
5) preparation of control line 7: rabbit anti-mouse igg antibody is pressed the concentration of 8mg/ml, peristaltic pump is awarded liquid measure 0.4ml/min, line speed 50m/20min, and 20 ℃ of forced air dryings are 12 hours in drying box.
6) coated film 4 preparations: will contain the cellulose nitrate NC film of detection line and control line with above-mentioned confining liquid in 37 ℃ of sealings 60 minutes, take out rearmounted 37 ℃ of lower drying and processings two hours, envelope is for subsequent use.
The preparation of the polyester film of coated with gold labeling antibody:
1) preparation of aqueous solution of chloraurate: with the dissolving of the tri-distilled water of 10g gold chloride 1000ml, be made into 1% aqueous solution, place 4 ℃ for subsequent use, the term of validity 3 months.
2) preparation of trisodium citrate: dissolve trisodium citrate with tri-distilled water, be made into 1% aqueous solution, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity 7 days.
3) preparation of 1% wet chemical: 1g sal tartari is dissolved with tri-distilled water with 100ml, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity is 7 days.
4) golden labeling antibody is preserved the preparation of liquid: with the sucrose of 15g, the Sodium azide of 20ul, in the 1%BSA dissolving of the pH=7.4 of 100ml, with 0.22U membrane filtration mistake, place 4 ℃ for subsequent use, the term of validity is 7 days.
5) preparation of the particle of collaurum: 1% gold chloride is diluted to 0.01% with tri-distilled water, place 95 ℃ of reactions 10 minutes, add the 1ml trisodium citrate, continue reaction 15 minutes, until the colloidal gold solution color by indigo plant after purple stain is red, add the 2ml1% solution of potassium carbonate after the cooling for subsequent use.Outward appearance should be pure, and is bright, without precipitation and floating thing.
6) preparation of golden labeling antibody: regulate collaurum pH value to 7.5 with 1% solution of potassium carbonate, collaurum by 20 microgram monoclonal antibody M155/ml adds, mixing afterreaction 15 minutes, with 10000rpm centrifugal 10 minutes, remove supernatant, precipitation is sealed washed twice with 1%BSA, with mark cleansing solution washed twice, preserve the liquid dissolving with golden labeling antibody again, place 4 ℃ for subsequent use, the term of validity is 7 days.
7) collaurum that mark is good is layered on the polyester film uniformly, and discharge rate is the 0.9ul/cm*3 road, drying, and envelope, for subsequent use.
End liner 1, sample pad 2 and thieving paper 5 are the general parts in this area.Above-mentioned sample pad 2, the polyester film 3 that is coated with golden labeling antibody, coated film 4 and thieving paper 5 are overlapped stickup in turn mutually obtain test paper plate, the test strips that at last test paper plate is cut into different in width gets final product.
Two kinds of method sensitivity and specificity are relatively
Remarks: "+" expression positive findings, the quantitaes colour developing degree of "+" i.e. is the order of severity of the positive, and more representatives colour developings are that positive severity is higher more deeply, and it is negative findings that "-" expression does not develop the color.
The colloidal gold strip of the large system of both sides of the present invention can significantly improve detection sensitivity and specificity as seen from the above table.
The monoclonal antibody of determined antigen of the present invention is not limited to employed cardiac muscle troponin I monoclonal antibody 4C2 and M155 among the embodiment, and any antibody that can be combined with determined antigen or antibody or antigen all can be applied to the two amplification systems of the present invention.In addition, the present invention is not limited to the collaurum field that is applied in, and also can be used for the immune response in fluorescence field and latex field.
In above embodiment, various processes and the method do not described in detail are conventional methods as known in the art.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (2)
1. the colloidal gold strip of two amplification systems, comprise the test strips end liner, and mutually overlap in turn sample pad, the polyester film that is coated with golden labeling antibody, coated film and the thieving paper of pasting on the test strips end liner, it is characterized in that a described coated film parallel coated streptavidin-determined antigen monoclonal antibody F(ab that is provided with in middle part)
2The control line of the detection line of fragment and a coated rabbit anti-mouse igg antibody has been coated with determined antigen monoclonal antibody F(ab in the described sample pad)
2Fragment-biotin, described golden labeling antibody are colloid gold label ProteinA-determined antigen monoclonal antibody; The F(ab of described streptavidin-determined antigen monoclonal antibody)
2Fragment is the F(ab of streptavidin and cardiac muscle troponin I monoclonal antibody)
2After the fragment coupling in conjunction with product; The ProteinA-myocardium protein I monoclonal antibody that described colloid gold label ProteinA-determined antigen monoclonal antibody is colloid gold label.
2. the preparation method of a colloidal gold strip claimed in claim 1 is characterized in that comprising the steps:
1) preparation coated film is with the F(ab of coated damping fluid dilution streptavidin-determined antigen monoclonal antibody)
2Fragment and rabbit anti-mouse igg antibody, and two kinds of antibody after will diluting are sprayed on respectively the middle part of coated film abreast, two kinds of antibody infiltrate the control line that forms respectively determined antigen detection line and rabbit anti-mouse igg antibody behind the coated film;
2) polyester film of preparation coated with gold labeling antibody: by gold grain mark ProteinA, the Fc fragment non-specific binding of the monoclonal antibody by ProteinA and determined antigen is combined in the monoclonal antibody of determined antigen on the gold grain indirectly;
3) preparation determined antigen monoclonal antibody F(ab)
2The sample pad that fragment-biotin is coated;
4) test strips assembling;
Wherein, the F(ab of described streptavidin-determined antigen monoclonal antibody)
2Fragment is the F(ab of streptavidin and cardiac muscle troponin I monoclonal antibody)
2After the fragment coupling in conjunction with product;
Step 2) described golden labeling antibody is by colloid gold particle mark ProteinA, by the Fc section non-specific binding of ProteinA and cardiac muscle troponin I monoclonal antibody, with indirectly being combined on the colloid gold particle that the cardiac muscle troponin I monoclonal antibody is amplified.
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| CN102841208B (en) * | 2011-06-24 | 2014-11-26 | 北京乐普医疗科技有限责任公司 | Colloidal gold test paper for quickly detecting troponin I and preparation method for colloidal gold test paper |
| CN102841207A (en) * | 2011-06-24 | 2012-12-26 | 北京乐普医疗科技有限责任公司 | Fluorescent immune chromatographic test strip for quantitively detecting troponin I and preparation method thereof |
| ES2702033T3 (en) | 2011-11-21 | 2019-02-27 | Abay Sa | Signal amplification in immunoassays |
| WO2013082943A1 (en) * | 2011-12-06 | 2013-06-13 | Li Jiutong | Fluorescence assay and device |
| CN102590517A (en) * | 2012-01-19 | 2012-07-18 | 南京基蛋生物科技有限公司 | Immunochromatography test paper and preparation method thereof |
| CN103293303B (en) * | 2012-03-01 | 2016-12-14 | 上海鑫谱生物科技有限公司 | A kind of fluorescence analysis method and device |
| CN104076140B (en) * | 2014-07-09 | 2017-01-11 | 国家纳米科学中心 | Construction and application of chemiluminiscence-colloidal gold immunochromatography test paper |
| TWI691716B (en) | 2014-08-13 | 2020-04-21 | 美商艾巴希斯公司 | Signal amplification in plasmonic specific-binding partner assays |
| CN104714018A (en) * | 2015-04-14 | 2015-06-17 | 武汉华美生物工程有限公司 | Colloidal gold micropore type detecting kit and preparation method thereof |
| JP6944438B2 (en) | 2015-08-04 | 2021-10-06 | ゾエティス サービシズ リミテッド ライアビリティ カンパニー | Signal amplification in solution-based plasmon-specific binding partner assay |
| CN105929154A (en) * | 2016-04-22 | 2016-09-07 | 深圳真瑞生物科技有限公司 | Test strip and kit for rapid detection of antibody against brucellosis |
| IL295118A (en) | 2017-01-30 | 2022-09-01 | Zoetis Services Llc | Solution-based plasmon specific-binding partner assays and metallic nanostructures |
| CN106932567B (en) * | 2017-02-27 | 2018-10-30 | 天津市泌尿外科研究所 | A kind of preparation method for the immune targeted nano gold that recombinant protein A mediates |
| CN106970219A (en) * | 2017-04-28 | 2017-07-21 | 北京金豪制药股份有限公司 | One kind is based on HIV in colloidal gold method detection urine（1+2）Antibody reagent |
| CN109856407B (en) * | 2018-12-26 | 2022-11-18 | 北京勤邦生物技术有限公司 | Canine distemper virus antibody fluorescence detection test strip and preparation method and application thereof |
| CN111896747A (en) * | 2020-08-02 | 2020-11-06 | 广州德成生物科技有限公司 | Immunochromatography test paper for detecting novel coronavirus IgM and IgG |
| CN113834934A (en) * | 2021-11-29 | 2021-12-24 | 北京百普赛斯生物科技股份有限公司 | Immunochromatography test strip and preparation method thereof |
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| CN1166953C (en) * | 2001-05-30 | 2004-09-15 | 上海晶泰生物技术有限公司 | Fast gold mark test reagent kit for one-step multiple-index test of myocardial infarction |
| EP1882184A4 (en) * | 2005-05-20 | 2008-07-30 | Calypte Biomedical Corp | Oral fluid rapid immunochromatography test |
| CN1982337A (en) * | 2005-12-14 | 2007-06-20 | 中国医学科学院放射医学研究所 | Synthesis of polypeptide antibody from anti-human myocardial troponin I, its production and use |
| CN101105497B (en) * | 2006-07-13 | 2011-10-19 | 上海凯创生物技术有限公司 | Cardiac muscle troponin I detection reagent kit and its preparation method |
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