CN102091100B - Method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris - Google Patents
Method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris Download PDFInfo
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- CN102091100B CN102091100B CN200910218363A CN200910218363A CN102091100B CN 102091100 B CN102091100 B CN 102091100B CN 200910218363 A CN200910218363 A CN 200910218363A CN 200910218363 A CN200910218363 A CN 200910218363A CN 102091100 B CN102091100 B CN 102091100B
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Abstract
The invention relates to a method for improving main medicinal ingredients through solid fermentation of Indian buead and cordyceps militaris. The method mainly comprises the following steps of: (1) preparing Indian buead and cordyceps militaris strains; (2) preparing a solid fermentation culture medium of the Indian buead and the cordyceps militaris; and (3) inoculating and culturing the Indian buead and the cordyceps militaris mycelium, post-treating and detecting medicinal ingredient content. Synthetic capacity of the main medicinal ingredients of the cordyceps militaris is improved by fully utilizing abundant enzyme systems of the Indian buead and the cordyceps militaris. The method is simple and convenient in operation and good in effect, and can be popularized and applied on a large scale.
Description
Technical field
The invention belongs to the microbial fermentation technology field, relate to a kind of method that adopts Poria cocos and Cordyceps militaris (L.) Link. to ferment altogether to improve main pharmaceutical compound.
Background technology
Cordyceps militaris (L.) Link. (Cordyceps militaris L.Link) belongs to xenogenesis together with entomophyte, is the medicine-food two-purpose fungi of generally acknowledging both at home and abroad.Cordyceps militaris (L.) Link. contains cordycepin, adenosine, polysaccharide, lectin isoreactivity material, has anti-cancer, and anti-inflammatory is antiviral, effects such as enhancing body immunizing power.Among the peoplely be used for pneumonia, suffer from a deficiency of the kidney, treatment of diseases such as pain in the back, its in reducing blood-fat, prevent and treat arteriosclerosis, protection heart and brain tissues, tranquilizing soporific, enhancing macrophage activity, aspects such as anticancer, anti-inflammatory, antibiotic, anoxia have good effects.Adopting the mycelial technological method of artificial culture Cordyceps militaris (L.) Link., can obtain to be higher than the Chinese caterpillar fungus culture of natural Cordyceps militaris (L.) Link. pharmaceutical use at short notice, reduce the cost that people take Cordyceps militaris (L.) Link. greatly, is the effective means that effectively solves the Chinese caterpillar fungus imbalance between supply and demand.
Poria cocos (Poria cocos (schw.) wolf) is the traditional rare traditional Chinese medicine of China, and according to Compendium of Material Medica record, Poria cocos slightly sweet flavor, property are flat, the thoughts of returning home, lung, the spleen channel, the tool invigorating the spleen, calm the nerves, improve looks, calmness, diuretic properties, also can strengthen the health immunological competence.The main chemical composition of Poria cocos comprises pachyman, triterpene substance and protein etc.
For improving the content of the main pharmaceutical compound of Cordyceps militaris (L.) Link. fungus filament, inoculation Poria cocos bacterial classification in Chinese caterpillar fungus culture medium makes full use of the two abundant enzyme system, effectively transforms culture medium.Zhou Lihong etc. have reported the method that monascus and Cordyceps militaris (L.) Link. solid ferment altogether, cordycepin content improved 2.5 times (Zhou Lihong etc., edible mushrooms, 2008,3:40-42).The present invention makes full use of the advantage that the Poria cocos bacterial classification efficiently transforms carbon source and nitrogenous source in the substratum, promotes the Cordyceps militaris (L.) Link. growth, for the accumulation of Cordyceps militaris (L.) Link. fungus filament meta-bolites lays the foundation, the biosynthesis ability of the main pharmaceutical compound of Cordyceps militaris (L.) Link. is significantly strengthened.
Summary of the invention
The invention provides a kind of method that adopts Poria cocos and Cordyceps militaris (L.) Link. solid to ferment altogether to improve main pharmaceutical compound.
The operation steps of this method is following:
(1) preparation of bacterial classification: Cordyceps militaris spawn of preserving on the PDA slant agar substratum and Poria cocos bacterial classification are inoculated into liquid PDA substratum respectively, 25 ℃ of culture temperature, shaking speed 120r/min, activation culture 4 days, for use;
(2) preparation of fermention medium altogether: by the substratum PC-1 that preparation Poria cocos and Cordyceps militaris (L.) Link. shown in the table 1 are fermented altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Substratum is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, 121 ℃ of autoclaving 60min, activatory Cordyceps militaris spawn and Poria cocos bacterial classification in the cooling back inoculation step (1), mixing.The Cordyceps militaris spawn inoculum size is 5%～15% of a substratum gross weight, and Poria cocos bacterial classification inoculum size is 5%～15% of a substratum gross weight.The dark cultivation, 25 ℃ of culture temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
Table 1PC-1 medium component
Annotate: above content is mass percent.
Key point of the present invention is: the inoculum size of Poria cocos bacterial classification and Cordyceps militaris spawn in the substratum, above key point if can be got hold of, and can significantly improve Poria cocos and the Cordyceps militaris (L.) Link. solid main pharmaceutical ingredient content of fermenting culture altogether.
The present invention is easy and simple to handle, and is respond well, may be used on large-scale promotion Poria cocos and Cordyceps militaris (L.) Link. solid and is total in the actual production process of fermentative prodn.
Embodiment
Following examples are used to explain the present invention, but are not limitations of the present invention.
Embodiment 1:
(1) preparation of bacterial classification: Cordyceps militaris spawn of preserving on the PDA slant agar substratum and Poria cocos bacterial classification are inoculated into liquid PDA substratum respectively, 25 ℃ of culture temperature, shaking speed 120r/min, activation culture 4 days, for use;
(2) preparation of fermention medium altogether: by the substratum PC-1 that preparation Poria cocos and Cordyceps militaris (L.) Link. shown in the table 1 are fermented altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Substratum is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, and 121 ℃ of autoclaving 60min inoculate activatory Cordyceps militaris spawn and Poria cocos bacterial classification, mixing after the cooling.The Cordyceps militaris spawn inoculum size is 5% of a substratum gross weight, and Poria cocos bacterial classification inoculum size is 10% of a substratum gross weight.The dark cultivation, 25 ℃ of culture temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria cocos and the Cordyceps militaris (L.) Link. solid content of the main pharmaceutical compound of fermenting culture altogether, its result sees table 2.
The main pharmaceutical ingredient content detected result of table 2 inoculation 10% Poria cocos and 5% Cordyceps militaris spawn culture
Embodiment 2:
(1) preparation of bacterial classification: Cordyceps militaris spawn of preserving on the PDA slant agar substratum and Poria cocos bacterial classification are inoculated into liquid PDA substratum respectively, 25 ℃ of culture temperature, shaking speed 120r/min, activation culture 4 days, for use;
(2) preparation of fermention medium altogether: by the substratum PC-1 that preparation Poria cocos and Cordyceps militaris (L.) Link. shown in the table 1 are fermented altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Substratum is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, and 121 ℃ of autoclaving 60min inoculate activatory Cordyceps militaris spawn and Poria cocos bacterial classification, mixing after the cooling.The Cordyceps militaris spawn inoculum size is 10% of a substratum gross weight, and Poria cocos bacterial classification inoculum size is 5% of a substratum gross weight.The dark cultivation, 25 ℃ of culture temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria cocos and the Cordyceps militaris (L.) Link. solid content of the main pharmaceutical compound of fermenting culture altogether, its result sees table 3.
The main pharmaceutical ingredient content detected result of table 3 inoculation 5% Poria cocos and 10% Cordyceps militaris spawn culture
Embodiment 3:
(1) preparation of bacterial classification: Cordyceps militaris spawn of preserving on the PDA slant agar substratum and Poria cocos bacterial classification are inoculated into liquid PDA substratum respectively, 25 ℃ of culture temperature, shaking speed 120r/min, activation culture 4 days, for use;
(2) preparation of fermention medium altogether: by the substratum PC-1 that preparation Poria cocos and Cordyceps militaris (L.) Link. shown in the table 1 are fermented altogether, material-water ratio is 1: 1.2, and adjust pH is 5.8.Substratum is packed into by the amount of 60 gram/bottles in the 500ml triangular flask, and 121 ℃ of autoclaving 60min inoculate activatory Cordyceps militaris spawn and Poria cocos bacterial classification, mixing after the cooling.The Cordyceps militaris spawn inoculum size is 15% of a substratum gross weight, and Poria cocos bacterial classification inoculum size is 10% of a substratum gross weight.The dark cultivation, 25 ℃ of culture temperature were cultivated after 15 days, and the taking-up culture is dried to constant weight in 40 ℃, is crushed to 80 order powder then.
(3) measure Poria cocos and the Cordyceps militaris (L.) Link. solid content of the main pharmaceutical compound of fermenting culture altogether, its result sees table 4.
The main pharmaceutical ingredient content detected result of table 4 inoculation 15% Poria cocos and 15% Cordyceps militaris spawn culture
Claims (1)
1. method that adopts Poria cocos and Cordyceps militaris (L.) Link. solid to ferment altogether to improve main pharmaceutical compound is characterized in that the operation steps of this method comprises:
(1) preparation of bacterial classification: Cordyceps militaris spawn of preserving on the PDA slant agar substratum and Poria cocos bacterial classification are inoculated into liquid PDA substratum respectively, 25 ℃ of culture temperature, shaking speed 120r/min, activation culture 4 days, for use;
(2) preparation of fermention medium altogether: the composition of the PC-1 substratum that preparation Poria cocos and Cordyceps militaris (L.) Link. are fermented altogether is calculated by weight to: Semen Maydis powder 25%, bran 30%, pine wood chip 30%, analysis for soybean powder 14.85%, KH
2PO
40.05%, MgSO
40.10%, material-water ratio is 1: 1.2, and adjust pH is 5.8;
(3) substratum is packed in the 500ml triangular flask by the amount of 60 gram/bottles, and 121 ℃ of autoclaving 60min inoculate activatory Cordyceps militaris spawn and Poria cocos bacterial classification after the cooling; Mixing, Cordyceps militaris spawn inoculum size are 5%～15% of substratum gross weight, and Poria cocos bacterial classification inoculum size is 5%～15% of a substratum gross weight; The dark cultivation, 25 ℃ of culture temperature are cultivated after 15 days; Take out culture, be dried to constant weight in 40 ℃, be crushed to 80 order powder then.
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| CN102293122B (en) * | 2011-06-24 | 2012-07-25 | 王满玉 | Cultivating method for cordyceps militaris by using manyprickle acathopanax root |
| CN104686199A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis highland barley |
| CN109689879A (en) * | 2016-09-06 | 2019-04-26 | 杏辉天力（杭州）药业有限公司 | Poria cocos tunning and its preparation method |
| CN107586726A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris rhizoma Gastrodiae liquid fermentation method and the application of cordycepin content |
| CN107586811A (en) * | 2017-10-13 | 2018-01-16 | 贵阳中医学院 | Improve Cordyceps militaris bark of eucommia liquid fermentation method and the application of cordycepin content |
| CN112136972A (en) * | 2019-06-28 | 2020-12-29 | 鲁东大学 | Method for producing feed additive by combined fermentation of cordyceps militaris and traditional Chinese medicines |
| CN113730476A (en) * | 2021-09-17 | 2021-12-03 | 无锡康能特医食品科技有限公司 | Paecilomyces hepiali strain fermentation composition and preparation method and application thereof |
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| 周礼红，蒋春玲.红曲霉与蛹虫草固体共发酵初步研究.《食用菌》.2008,第2008年卷(第3期),40-42页. * |
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