CN102068714A - Collagen sponge and preparation method thereof - Google Patents
Collagen sponge and preparation method thereof Download PDFInfo
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- CN102068714A CN102068714A CN2011100220084A CN201110022008A CN102068714A CN 102068714 A CN102068714 A CN 102068714A CN 2011100220084 A CN2011100220084 A CN 2011100220084A CN 201110022008 A CN201110022008 A CN 201110022008A CN 102068714 A CN102068714 A CN 102068714A
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Abstract
The invention discloses collagen sponge and a preparation method thereof. The collagen sponge does not contain a chemical cross-linking agent, has the porosity of over 90 percent, and is prepared by performing radiation cross-linking on aqueous solution of collagen and then freeze-drying the aqueous solution of collagen; and sterilization is performed during cross-linking so that the biosafety problem caused during preparation is solved. The collagen sponge prepared by the method has no residue of chemical reagents, better biocompatibility and structural uniformity, and adjustable degradation rate and mechanical strength, and can be used as biomedical materials such as dressing, tissue engineering stents, artificial tissue components, tissue fillers, embolic agents and the like.
Description
Technical field
The present invention relates to the preparation of biomaterial, particularly a kind of collagen sponge and preparation method thereof, this collagen sponge is applicable to but is not limited to biomedical materials field.
Background technology
Collagen is the celloglobulin family the widest, that content is the abundantest, kind is more that distributes in the animal body, and the collagen that is present in the various organ-tissues accounts for more than 30% of human body protein total amount.As the main component of extracellular matrix, collagen is regarded as the most useful biomaterial.Low, biodegradable, the easy absorption of collagen antigenicity, nontoxic, good biocompatibility, and have anastalsis, in tissue repair, playing the part of important role.
Known, collagen sponge is used for artificial skin is implanted in site of injury, by its loose structure and this role in tissue repair of collagen that is suitable for the cell growth, can promote cell proliferation and then accelerate organization healing speed.Mostly traditional collagen sponge is not carry out crosslinking Treatment or crosslinked with chemical cross-linking agents such as glutaraldehydes by the collagen solution lyophilization is made.Do not carry out the too fast and insufficient strength of crosslinked collagen sponge degraded; The residual toxicity of chemical cross-linking agents such as glutaraldehyde can have a strong impact on cell growth and tissue regeneration, thereby influences therapeutic effect.
Chinese invention patent prospectus CN101066469A provides a kind of preparation method of collagen mandruka.This invention is mixed in proportion the acetic acid solution of collagen and chitosan, and the control freeze drying process obtains the porous collagen protein sponge.But this technology is not carried out crosslinking Treatment to collagen, and degradation rate of the sponge that obtains and mechanical strength are very restricted in actual applications.
The preparation method of the medical collagen sponge that Chinese invention patent ublic specification of application CN1416907A provides is that collagen solution is mixed with glycerol, and lyophilization obtains spongy material, again it is fixedly obtained medical collagen sponge down at 100-150 ℃.This method realizes that the method for collagen cross-linking is the heat cross-linking method, can cause collagenous degeneration, influences its biological activity.
Chinese invention patent ublic specification of application CN101005865A provides the manufacture method of two kinds of collagen sponges, is intended to reach high qualification rate by simple and easy technology.Add chloroform behind the pH of this invention with acetic acid/hydrochloric acid adjusting collagen solution, freezing collagen expanding foam solution, drying obtains collagen sponge, and the reuse glutaraldehyde solution is fumigated and is obtained the crosslinked with collagen sponge; Another kind method is that the collagen diluent lyophilization that will contain glutaraldehyde obtains collagen sponge.Though this method has realized the crosslinked of collagen sponge, use therein foaming agent and glutaraldehyde cross-linking agent meeting are residual in material, and these chemical reagent will have a strong impact on the biocompatibility of collagen.
The preparation method of the collagen sponge membrane that provides among the Chinese invention patent prospectus CN1587391A is with collagen and the lyophilization of 6-chondroitin sulfate cellulose solution elder generation, makes collagen sponge membrane in 105 ℃ of dryings again, and is crosslinked with realizing in this film immersion glutaraldehyde solution.Though water-washing process is arranged, unreacted glutaraldehyde can not be removed fully, can influence the histocompatibility of goods inevitably.
Summary of the invention
The present invention is intended to abandon the traditional handicraft of using a large amount of poisonous chemical agents, and a kind of collagen sponge that adopts green synthesis process to obtain is provided, and its good biocompatibility, structural homogeneity are good.
Another object of the present invention is to provide a kind of preparation method of collagen sponge, this method is carried out lyophilization after adopting crosslinking with radiation again, and preparation process is sterilized simultaneously, the safety problem of having avoided preparation process to cause.With the method can obtain that biocompatibility is better, structural homogeneity is better, degradation rate and the adjustable collagen sponge of mechanical strength.
For realizing the object of the invention to solve the technical problems, by the following technical solutions:
A kind of collagen sponge does not contain chemical cross-linking agent, and porosity is by earlier collagenic aqueous solution being carried out crosslinking with radiation, carrying out lyophilization then and be prepared from more than 90%.
Can also contain the poly-polysaccharide of water-soluble natural in the described collagen sponge, the weight ratio of the poly-polysaccharide of collagen protein and water-soluble natural is 9: 1～1: 9, is preferably 8: 2～4: 6.
The poly-polysaccharide of described water-soluble natural can be selected from one or more in Aloe polysaccharide, carboxymethyl chitosan, hydroxypropyl chitosan, sodium alginate, propylene glycol alginate, carboxymethyl cellulose, hydroxypropyl emthylcellulose, the carboxymethyl hydroxypropyl cellulose.
Can also contain somatomedin and/or medicine in the described collagen sponge to promote wound healing and tissue regeneration, somatomedin and content of medicines are decided according to concrete application requirements.
Described somatomedin can select fibroblast growth factor that those skilled in the art use always, hEGF etc. can promote the somatomedin that skin tissue regeneration is repaired.
Described medicine can be selected medicines such as this area disinfectant commonly used, antibiotic, burn-scald medicine, thromboembolism preventing suppository, makes it to have corresponding auxiliary treatment effect, and its consumption can adopt this area conventional amount used.
The preparation method of collagen sponge of the present invention is: collagen protein is made collagenic aqueous solution separately or with other component blend, pack then and carry out crosslinking with radiation and become collagen hydrogel, carry out lyophilization again and obtain collagen sponge.
The concentration expressed in percentage by weight of collagen protein is 0.2%-10% in the described collagenic aqueous solution, is preferably 0.75%～1.5%.Described collagen protein generally is a molecular weight greater than 3000 collagen protein.Described other component includes but not limited to that water-soluble natural gathers polysaccharide, somatomedin and medicine.In collagenic aqueous solution, the ratio of the poly-polysaccharide of collagen protein and water-soluble natural is 9: 1～1: 9, is preferably 8: 2～4: 6; The content of somatomedin is generally 0.001%～0.02% (weight); Medicament contg is decided according to the kind and the scope of application of medicine.
Described crosslinking with radiation carries out crosslinking with radiation for adopting gamma-rays, electron beam or ultraviolet.
When described crosslinking with radiation adopted gamma-rays or electron beam, irradiation dose was 3～125kGy, is preferably 3～30kGy.
When described crosslinking with radiation was ultraviolet, wavelength was 200-400nm, and power is 1.11～135mW/cm
2, operating distance is 1-20cm, exposure time is 30 minutes～24 hours.
Described cryodesiccated temperature is at-10～-80 ℃, preferred-20～-60 ℃.
If necessary, porous collagen sponge can be pulverized, sieved, obtain the graininess collagen sponge of different-grain diameter.
Collagen sponge of the present invention can be used as bleeding-stopping dressing, burns wound dressing, ulcer dressing etc.The porous collagen material also can be used as tissue engineering bracket, promotes cell proliferation, tissue repair.Cover the macromolecule membrane with water penetration on the collagen sponge that the present invention obtains, form permeable regulating course, this double-decker can be used for large tracts of land holostrome skin injury, as artificial dermis.The collagen sponge that the present invention obtains also can be used for the stomach wall reparation, plays the effect of anti, also can be used as purposes such as tissue filler in plastic surgery operations.The collagen sponge granule can be used as suppository and is used for various interventional therapys, as artery embolization for treatment of hysteromyoma, hemangioma, arteriovenous malformotion, malignant tumor and various massive hemorrhage etc.
The collagen sponge that utilizes method provided by the invention to obtain does not have chemical agent residue, has better biocompatibility and structural homogeneity.Fibroblast growing state on collagen porous support of the present invention is good, and the rate of increase was greater than 100% in 2 days.
Collagen sponge of the present invention and preparation method thereof has following advantage and beneficial effect at least:
(1) the present invention adopts natural macromolecular collagen, and does not add the material that acetic acid etc. has skin irritation in the solvent.
(2) with the poly-polysaccharide-modified collagen of water-soluble natural, can increase the swelling behavior and the pliability of collagen sponge, and can promote the granulation growth, use very crucial as dressing it.
(3) replace with chemical crosslinking technology such as glutaraldehydes with the crosslinking with radiation technology generation, can avoid the chemical agent residue toxicity of product, play the effect of sterilization simultaneously.
(4) the tropocollagen molecule amount selected for use of the present invention is bigger, can obtain good cross-linking effect at low concentration.
(5) porous material that obtains with the crosslinking with radiation technology has better structural homogeneity, helps the adhesion and the skin tissue regeneration of skin tissue cell.
(6) collagen porous material antienzyme degradation capability and the mechanical strength that obtains with radiotechnology significantly improves, and can regulate by control preparation technology.
(7) aperture of the adjustable porous material of control freeze drying process.
(8) controllability of this process is fine, and the structural homogeneity that obtains product is good than chemical crosslink technique also, has special advantages as bio-medical material.
Description of drawings
Fig. 1 is the sem photograph of the prepared collagen sponge of embodiment 6.
The specific embodiment
Below by embodiment the present invention is described in further detail, but the scope that does not limit the present invention in any way.
Embodiment 1
The moisture collagen of 5g (moisture about 98%) is mixed with 5g water, stir, obtain solid content and be 1% aqueous solution with the deaeration blender.With the aqueous solution test tube of packing into, remove bubble, seal the back with cobalt-60 irradiation 25kGy, obtain collagen hydrogel.This collagen hydrogel-60 ℃ of lyophilizations, is obtained collagen sponge.
Embodiment 2
With moisture collagen (the moisture about 98%) test tube of packing into, remove bubble, sealing and being placed on wavelength is 254nm, power is 1.66mW/cm
2Under the ultraviolet device, operating distance is 5cm, and irradiation 4 hours obtains collagen hydrogel.This collagen hydrogel-20 ℃ of lyophilizations, is obtained collagen sponge.
Embodiment 3
The 1 moisture collagen of gram (moisture about 98%) is mixed with 9g water, stir, obtain solid content and be 0.2% aqueous solution with the deaeration blender.With the aqueous solution test tube of packing into, remove bubble, seal the back and use electron beam irradiation 125kGy, obtain collagen hydrogel.This collagen hydrogel-60 ℃ of lyophilizations, is obtained collagen sponge.
Embodiment 4
0.1 gram carboxymethyl cellulose, the 20 moisture collagens of gram (moisture about 98%) are mixed, and the test tube of packing into after stirring with the deaeration blender removes bubble, seals the back with cobalt-60 irradiation 50kGy, obtains collagen-carboxymethyl cellulose hydrogel.This collagen-carboxymethyl cellulose hydrogel-60 ℃ of lyophilizations, is obtained sponge.
Embodiment 5
With moisture collagen (the moisture about 98%) test tube of packing into, remove bubble, seal back cobalt-60 irradiation 5kGy, obtain collagen hydrogel, measure the about 30kPa of its modulus of compressibility, in 0.1mg/ml collagen protein enzymatic solution, one hour mass loss rate about 70%.This collagen hydrogel-60 ℃ of lyophilizations, is obtained the collagen sponge of porous network structure.
Embodiment 6
With moisture collagen (the moisture about 98%) test tube of packing into, remove bubble, seal back cobalt-60 irradiation 15kGy, obtain collagen hydrogel, measure the about 35kPa of its modulus of compressibility, in 0.1mg/ml collagen protein enzymatic solution, one hour mass loss rate about 20%.This collagen hydrogel-20 ℃ of lyophilizations, is obtained the collagen sponge of porous network structure, as shown in Figure 1.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a collagen sponge does not contain chemical cross-linking agent, and porosity is more than 90%, is by collagenic aqueous solution is carried out crosslinking with radiation, and lyophilization is prepared from then.
2. collagen sponge as claimed in claim 1 is characterized in that, contains the poly-polysaccharide of water-soluble natural in the described collagen sponge, and wherein the weight ratio of the poly-polysaccharide of collagen protein and water-soluble natural is 9: 1～1: 9.
3. collagen sponge as claimed in claim 2, it is characterized in that the poly-polysaccharide of described water-soluble natural is selected from one or more in Aloe polysaccharide, carboxymethyl chitosan, hydroxypropyl chitosan, sodium alginate, propylene glycol alginate, carboxymethyl cellulose, hydroxypropyl emthylcellulose and the carboxymethyl hydroxypropyl cellulose.
4. collagen sponge as claimed in claim 1 is characterized in that, contains somatomedin and/or medicine in the described collagen sponge.
5. the preparation method of a collagen sponge is made collagenic aqueous solution separately or with other component blend with collagen protein, packs then to carry out crosslinking with radiation and become collagen hydrogel, and lyophilization obtains collagen sponge again.
6. preparation method as claimed in claim 5 is characterized in that, the concentration expressed in percentage by weight of collagen protein is 0.2%-10% in the described collagenic aqueous solution, and the molecular weight of described collagen protein is greater than 3000.
7. preparation method as claimed in claim 5 is characterized in that, described other component is poly-polysaccharide, somatomedin and/or a medicine of water-soluble natural, and wherein, in collagenic aqueous solution, the ratio of the poly-polysaccharide of collagen protein and water-soluble natural is 9: 1～1: 9; The weight percentage of somatomedin is 0.001%～0.02%.
8. preparation method as claimed in claim 5 is characterized in that, described crosslinking with radiation carries out crosslinking with radiation for adopting gamma-rays, electron beam or ultraviolet.
9. preparation method as claimed in claim 8 is characterized in that, adopting the irradiation dose of gamma-rays or crosslinking electron beam irradiation is 3～125kGy; Adopting the crosslinked ultraviolet wavelength of ultraviolet radiation is 200-400nm, and power is 1.11～135mW/cm
2, operating distance is 1-20cm, exposure time is 30 minutes～24 hours.
10. preparation method as claimed in claim 5 is characterized in that, described cryodesiccated temperature is at-10～-80 ℃.
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| CN102228715A (en) * | 2011-06-10 | 2011-11-02 | 青岛明药堂医药科技开发有限公司 | Liquid dressing containing chitosan derivative and preparation method thereof |
| CN102698313A (en) * | 2012-01-11 | 2012-10-03 | 北京大学 | Nano-silver antibacterial hydrogel and preparation method thereof |
| CN102908666A (en) * | 2012-10-11 | 2013-02-06 | 天津市赛瑞生物技术有限公司 | Collagen scaffold material for cornea |
| CN102989031A (en) * | 2012-11-07 | 2013-03-27 | 中国海洋大学 | High-expansibility medical polysaccharide-based material and application thereof |
| CN103007339A (en) * | 2012-12-06 | 2013-04-03 | 浙江医鼎医用敷料有限公司 | Biological powder medical dressing and preparation method thereof |
| CN103012859A (en) * | 2012-12-19 | 2013-04-03 | 青岛明月生物医用材料有限公司 | Chitosan and propylene glycol alginate blending material as well as preparation method and application thereof |
| CN103071181A (en) * | 2013-02-01 | 2013-05-01 | 刘昌桂 | Hydrogel as well as preparation method and purpose of hydrogel |
| CN104231288A (en) * | 2014-08-07 | 2014-12-24 | 厦门凝赋生物科技有限公司 | High-strength collagen gel and preparation method thereof |
| CN104353106A (en) * | 2014-11-05 | 2015-02-18 | 张自强 | Composition of fast hemostatic sponge gel and preparation method of composition |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6974679B2 (en) * | 2000-05-26 | 2005-12-13 | Coletica | Support with collagen base for tissue engineering and manufacture of biomaterials |
| US7108721B2 (en) * | 2000-05-11 | 2006-09-19 | Massachusetts Institute Of Technology | Tissue regrafting |
| CN1944495A (en) * | 2006-09-29 | 2007-04-11 | 北京大学 | Water gel containing natural high molecule and its radiation preparing method |
| CN101574539A (en) * | 2009-06-15 | 2009-11-11 | 北京大学 | Gelatin sponge and preparation method thereof |
-
2011
- 2011-01-19 CN CN2011100220084A patent/CN102068714A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7108721B2 (en) * | 2000-05-11 | 2006-09-19 | Massachusetts Institute Of Technology | Tissue regrafting |
| US6974679B2 (en) * | 2000-05-26 | 2005-12-13 | Coletica | Support with collagen base for tissue engineering and manufacture of biomaterials |
| CN1944495A (en) * | 2006-09-29 | 2007-04-11 | 北京大学 | Water gel containing natural high molecule and its radiation preparing method |
| CN101574539A (en) * | 2009-06-15 | 2009-11-11 | 北京大学 | Gelatin sponge and preparation method thereof |
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