CN102061251A - Novel optical bioreactor - Google Patents
Novel optical bioreactor Download PDFInfo
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- CN102061251A CN102061251A CN2010105612716A CN201010561271A CN102061251A CN 102061251 A CN102061251 A CN 102061251A CN 2010105612716 A CN2010105612716 A CN 2010105612716A CN 201010561271 A CN201010561271 A CN 201010561271A CN 102061251 A CN102061251 A CN 102061251A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention relates to a novel optical bioreactor, which comprises a cylindrical reactor shell. The optical bioreactor is characterized in that: a light transmission guide tube is arranged in the cavity of the cylindrical reactor shell, a light emitting diode (LED) lamp capable of raising light intensity and temperature is arranged in the light transmission guide tube, and a light source xenon lamp is arranged on the periphery of the cylindrical reactor shell. The LED lamp can freely adjust the illumination intensity and the light color, the xenon lamp can adjust the growth environment temperature of alga, and inner circular current type air-liquid circulation is adopted, so the growth requirements of different oil producing alga culture organisms can be effectively met, and yield and quality of the oil producing alga organisms are greatly promoted.
Description
Technical field
The present invention relates to a kind of novel bioreactor, be applicable to and produce produce oil microalgae biology.
Background technology
China's exploitation substitutes in the fuel source of oil, and produce oil microalgae (Blang's grape algae, diatoms etc.) is in dominant position.The exemplary illumination automatic control bioreactor that design and running is large-scale reaches the little algae of commercial mass production produce oil, and isolate bio-fuel-oil from little algae, can be used as automobile fuel oil.Develop the small-sized primary stage of bioreactor place both at home and abroad at present, especially aspect equipment enlarging, yet there are no breakthrough.The bioreactor of pilot scale and production usefulness because of volume is big, generally make with the high-quality stainless steel, but natural lighting is not all right; As use the high boron glass manufacturing apparatus, too high also being difficult to of cost applies.Therefore, how addressing the above problem is research purpose of the present invention.
Summary of the invention
The object of the present invention is to provide a kind of novel bioreactor, this reactor can not only be regulated the illumination color, and can also regulate intensity of illumination, satisfy the needs of different algae culture environment, thereby the output of produce oil microalgae biology and quality are all promoted greatly.
The invention is characterized in: a kind of novel bioreactor, comprise reactor tubular shell, it is characterized in that: described reactor tubular shell inner cavity is equipped with the printing opacity thrust-augmenting nozzle, the LED lamp that can heighten light intensity and temperature is installed in the described printing opacity thrust-augmenting nozzle, and described cylindrical shell reactor tubular shell periphery is provided with the light source xenon lamp.
Advantage of the present invention: but the present invention utilizes LED fluorescent tube free adjustment intensity of illumination, utilize xenon lamp can obtain spectrum as the sun, and can regulate the growing environment temperature, adopt circulation form air feed, thereby can effectively satisfy the growth needs that different algaes are cultivated, and the output of produce oil microalgae biology and all liftings greatly of quality.
Description of drawings
Fig. 1 is an embodiment of the invention process flow sheet.
Embodiment
With reference to figure 1, a kind of novel bioreactor comprises reactor tubular shell 1, and described reactor tubular shell 1 inner chamber is equipped with printing opacity thrust-augmenting nozzle 2, the LED lamp 3 that can heighten light intensity and temperature is installed in the described printing opacity thrust-augmenting nozzle 2, and described reactor tubular shell 1 periphery is provided with light source xenon lamp 4.
Above-mentioned reactor tubular shell 1 lower lumen is equipped with the air nozzle 7 that is connected with external supply air line.
Above-mentioned supply air line comprises prefilter 8 successively along air flow, air flowmeter 9, and air filter 10 and valve part 11, described valve part 11 is connected with air nozzle 7.
Above-mentioned reactor tubular shell 1 lower, outer perimeter is arranged with chuck 5, and described chuck 5 bottoms are connected with the chilled(cooling) water supply (CWS) pipeline, and top is connected with the water coolant recycling pipeline.
Above-mentioned cooling water tube curb chilled(cooling) water supply (CWS) comprises magnetic valve 12 and valve part 13 on flowing to successively, the water side of described valve part 13 is connected with the water-in of chuck 5 bottoms, the feed-water end of described valve part 13 also is connected with a water circulating pump 14, and described water circulating pump 14 is connected with constant temperature water tank 15.
Above-mentioned water coolant recycling pipeline reclaims to flow to along water coolant and includes the magnetic valve 16 that is connected with chuck 5 top water outlets, and described water outlet also is connected with constant temperature water tank 15.
Also be connected with the steam supply pipeline of sterilization usefulness on the above-mentioned reactor tubular shell 1, described steam supply pipe curb steam supply comprises vapor filter 17 and valve part 18 on flowing to, and the vapour outlet of described valve part 18 is connected with reactor tubular shell 1 steam inlet.
The pH value that also is provided with on the above-mentioned reactor tubular shell 1 in order to high temperature resistant pH value detecting sensor to be installed detects hole 19, detects hole 20 in order to the dissolved oxygen that the dissolved oxygen amount detecting sensor is installed, in order to the thermometer hole 21 and the material-feeding port 22 of temperature detection sensor to be installed.
Above-mentioned LED lamp, xenon lamp, pH value detecting sensor, dissolved oxygen amount detecting sensor and temperature detection sensor are connected with the PLC Controlling System respectively.
Offer on above-mentioned reactor tubular shell 1 perisporium in order to the through hole of high boron glass 6 to be installed.
The present invention uses the high-quality stainless steel and makes the shell of photoreactor (reactor tubular shell), the thrust-augmenting nozzle that interior dress high boron glass is transparent, high gas speed sterile air are by upwards ejection of nozzle, gas-liquid upwards flows through interior thrust-augmenting nozzle earlier, after leaving thrust-augmenting nozzle, gas-liquid separation, liquid flows downward between thrust-augmenting nozzle and reactor tubular shell 1 annular space, has formed the gas common loop reactor.New type light source LED fluorescent tube is installed in (length is short slightly than bioreactor), LED fluorescent tube make it directly not contact with the produce oil micro-algae culture medium in the thrust-augmenting nozzle, LED lamp luminescence intensity 0 ~ 13600lx, light intensity is adjustable.The color of light also can be cultivated needs according to the little algae of produce oil and be changed.The heat of LED fluorescent tube heating can be discharged automatically.Because of thrust-augmenting nozzle transparence in the bioreactor reaches 90%, so the inside and outside produce oil micro-algae culture medium of thrust-augmenting nozzle of flowing through all can obtain abundant luminous energy.
The top cover of stainless steel bioreactor and end cover adopt big visor, and (visor is with containing B
2O
3Be main high strength, the manufacturing of high transmission rate high boron glass), xenon lamp (sun lamp) is installed at the visor place.
Automatically control section has adopted advanced PLC Controlling System, and main control parameters has: intensity of illumination (lx) and illumination color, feed supplement, pH, dissolved oxygen, temperature etc., wherein back three is on-line Control.
The above only is preferred embodiment of the present invention, and all equalizations of being done according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (10)
1. novel bioreactor, comprise reactor tubular shell, it is characterized in that: described reactor tubular shell inner cavity is equipped with the printing opacity thrust-augmenting nozzle, and the LED lamp that can heighten light intensity and temperature is installed in the described printing opacity thrust-augmenting nozzle, and described reactor tubular shell periphery is provided with the light source xenon lamp.
2. novel bioreactor according to claim 1 is characterized in that: described reactor tubular outer casing underpart inner chamber is equipped with the air nozzle that is connected with external supply air line.
3. novel bioreactor according to claim 2 is characterized in that: described supply air line comprises prefilter successively along air flow, air flowmeter, and air filter and valve part, described valve part is connected with air nozzle.
4. novel bioreactor according to claim 1 is characterized in that: described reactor tubular outer casing underpart periphery is arranged with chuck, and described chuck bottom is connected with the chilled(cooling) water supply (CWS) pipeline, and top is connected with the water coolant recycling pipeline.
5. novel bioreactor according to claim 4, it is characterized in that: described cooling water tube curb chilled(cooling) water supply (CWS) comprises magnetic valve and valve part on flowing to successively, the water side of described valve part is connected with the water-in of chuck bottom, the feed-water end of described valve part also is connected with a water circulating pump, and described water circulating pump is connected with constant temperature water tank.
6. according to claim 4 and 5 described novel bioreactors, it is characterized in that: described water coolant recycling pipeline reclaims to flow to along water coolant and includes the magnetic valve that is connected with chuck top water outlet, and described water outlet also is connected with constant temperature water tank.
7. novel bioreactor according to claim 1, it is characterized in that: the steam supply pipeline that also is connected with sterilization usefulness on the described reactor tubular shell, described steam supply pipe curb steam supply comprises vapor filter and valve part on flowing to, and the vapour outlet of described valve part is connected with reactor tubular shell steam inlet.
8. novel bioreactor according to claim 1, it is characterized in that: the pH value that also is provided with on the described reactor tubular shell in order to high temperature resistant pH value detecting sensor to be installed detects the hole, detect the hole in order to the dissolved oxygen that the dissolved oxygen amount detecting sensor is installed, in order to the thermometer hole and the material-feeding port of temperature detection sensor to be installed.
9. according to claim 1 and 8 described novel bioreactors, it is characterized in that: described LED lamp, xenon lamp, pH value detecting sensor, dissolved oxygen amount detecting sensor and temperature detection sensor are connected with the PLC Controlling System respectively.
10. novel bioreactor according to claim 1 is characterized in that: offer on the described reactor tubular case peripheral wall in order to the through hole of high boron glass to be installed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105612716A CN102061251A (en) | 2010-11-26 | 2010-11-26 | Novel optical bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105612716A CN102061251A (en) | 2010-11-26 | 2010-11-26 | Novel optical bioreactor |
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| CN102061251A true CN102061251A (en) | 2011-05-18 |
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| CN2010105612716A Pending CN102061251A (en) | 2010-11-26 | 2010-11-26 | Novel optical bioreactor |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783414A (en) * | 2011-05-19 | 2012-11-21 | 河南省海乐森药用细胞工程技术有限公司 | External circulation mist-type bioreactor for large-scale cultivation of plant hairy roots |
| CN103773673A (en) * | 2014-02-25 | 2014-05-07 | 中国科学院水生生物研究所 | Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof |
| CN104195033A (en) * | 2014-09-09 | 2014-12-10 | 青岛汇君环境能源工程有限公司 | Flat-plate type photobioreactor |
| CN108359580A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | A kind of microvesicle bioreactor for economic microdisk electrode |
| CN108395993A (en) * | 2018-02-28 | 2018-08-14 | 清华大学深圳研究生院 | A kind of application method of microvesicle bioreactor for economic microdisk electrode |
| CN109259180A (en) * | 2018-08-28 | 2019-01-25 | 季华实验室 | Brewing method, brewing apparatus and brewing system |
| CN109266513A (en) * | 2018-08-28 | 2019-01-25 | 季华实验室 | Brewing apparatus, brewing system and brewing method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2473210Y (en) * | 2001-03-26 | 2002-01-23 | 中国科学院化工冶金研究所 | Cell cultivation and air float collection coupling reactor |
| CN2878404Y (en) * | 2005-12-14 | 2007-03-14 | 中国科学院海洋研究所 | Two-direction counter current micro-alga culture device |
| CN101265450A (en) * | 2007-11-30 | 2008-09-17 | 天津科技大学 | Scale culture and promotion technique for trans TNF-alpha gene blue algae growth and gene expression by light reactor |
| CN101550394A (en) * | 2009-05-15 | 2009-10-07 | 武汉钢铁(集团)公司 | Airlift type photobioreactor |
-
2010
- 2010-11-26 CN CN2010105612716A patent/CN102061251A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2473210Y (en) * | 2001-03-26 | 2002-01-23 | 中国科学院化工冶金研究所 | Cell cultivation and air float collection coupling reactor |
| CN2878404Y (en) * | 2005-12-14 | 2007-03-14 | 中国科学院海洋研究所 | Two-direction counter current micro-alga culture device |
| CN101265450A (en) * | 2007-11-30 | 2008-09-17 | 天津科技大学 | Scale culture and promotion technique for trans TNF-alpha gene blue algae growth and gene expression by light reactor |
| CN101550394A (en) * | 2009-05-15 | 2009-10-07 | 武汉钢铁(集团)公司 | Airlift type photobioreactor |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783414A (en) * | 2011-05-19 | 2012-11-21 | 河南省海乐森药用细胞工程技术有限公司 | External circulation mist-type bioreactor for large-scale cultivation of plant hairy roots |
| CN103773673A (en) * | 2014-02-25 | 2014-05-07 | 中国科学院水生生物研究所 | Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof |
| CN103773673B (en) * | 2014-02-25 | 2015-02-18 | 中国科学院水生生物研究所 | Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof |
| CN104195033A (en) * | 2014-09-09 | 2014-12-10 | 青岛汇君环境能源工程有限公司 | Flat-plate type photobioreactor |
| CN108359580A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | A kind of microvesicle bioreactor for economic microdisk electrode |
| CN108395993A (en) * | 2018-02-28 | 2018-08-14 | 清华大学深圳研究生院 | A kind of application method of microvesicle bioreactor for economic microdisk electrode |
| CN108359580B (en) * | 2018-02-28 | 2020-04-21 | 清华大学深圳国际研究生院 | Microbubble photobioreactor for economic microalgae culture |
| CN108395993B (en) * | 2018-02-28 | 2021-06-15 | 清华大学深圳研究生院 | Use method of microbubble photobioreactor for economic microalgae culture |
| CN109259180A (en) * | 2018-08-28 | 2019-01-25 | 季华实验室 | Brewing method, brewing apparatus and brewing system |
| CN109266513A (en) * | 2018-08-28 | 2019-01-25 | 季华实验室 | Brewing apparatus, brewing system and brewing method |
| CN109259180B (en) * | 2018-08-28 | 2022-07-19 | 季华实验室 | Brewing method, brewing device and brewing system |
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Effective date of abandoning: 20110518 |
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