CN102048958B - Compound dendrobium candidum hypoglycemic composition and preparation method thereof - Google Patents
Compound dendrobium candidum hypoglycemic composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a compound dendrobium candidum hypoglycemic composition and a preparation method thereof. The compound dendrobium candidum hypoglycemic composition is prepared from the following Chinese traditional medicine raw materials in weight ratio: 2-40g of dendrobium candidum, 5-80g of prepared rehmannia root, 5-80g of Chinese wolfberry, 5-80g of dogwood, 5-80g of dodder, 5-80g of milk vetch, 5-80g of wolfberry bark, 2-40g of coptis, 5-80g of rhizoma atractylodis, 5-80g of gallnut, 5-80g of root-bark of tree peony and 2-40g of licorice. The compound dendrobium candidum hypoglycemic composition provided by the invention can improve the immunity of the human body, regulate the equilibrium between yin and yang of the human body, obviously reduce the blood sugar level, obviously improve the symptoms of diabetes, enhance the memory, nourish the consumptive disease of the five internal organs, resist the aging, inhibit the tumor and resist the anoxia, has an obvious effect on diabetes with deficiency of both qi and yin and blood stasis syndrome and vascular complications thereof, is suitable for treating diabetes complicated with coronary heart disease, cerebral vascular diseases, peripheral vascular diseases, retinopathy, diabetic nephropathy and the like, and does not have toxic or side effects.
Description
Technical field
The present invention relates to a kind of blood-sugar lowering tcm drug composition and method of making the same, specifically, relate to a kind of compound recipe Herba Dendrobii compound for reducing blood suger and preparation method thereof.
Background technology
Along with high development of social economy; The progressively raising of living standard, the continuous prolongation of the modernization of life pattern and the average life span, the sickness rate of countries in the world diabetes also progressively raises thereupon; Its chronic complicating diseases brings lifelong deformity to the patient, severe patient even cause death.Therefore the incidence and development of active prevention diabetes and delaying complications of diabetes has become the medical problem of global salience.
In March, 2010, the media releasing that chairman IDF Jean Claude professor Mbanya holds can go up, and has announced adult Chinese diabetes epidemiology situation; Announce that Chinese diabetics number is up to 9,240 ten thousand; Also have 1.482 hundred million adults to be in prediabetes, compare 5,080 ten thousand diabeticss of India, at present; Patient's number of China " has surpassed India, has become the epiphytotics " center " of world's diabetes.
World Economic Forum's global risk overview assessment report in 2009 points out, chronic disease is one of serious risk of facing of global economy.The Epidemiological study prediction of result that IDF is up-to-date, the expenditure of the Chinese government in this year aspect diabetes will reach 6,900,000,000 dollars, and expenditure increased by 1,900,000,000 dollars more in the past.And the WHO prediction, in 10 years of 2005-2015, China will reach 557,700,000,000 dollars in the expenditure of prevention and control diabetes and cardiovascular disease.No matter be IDF or the report of WHO, all show that to us diabetes will become financial burden heavy in the following Chinese development.
And aspect treatment, particularly the treatment situation of diabetics is but also pessimistic.It is comparatively desirable that only there is about one one-tenth patient's glycemic control in China at present.And by the complication that diabetes cause, like pedopathy, nephropathy, oculopathy, encephalopathy, heart disease, dermatosis, sexually transmitted disease (STD) etc., be the principal element that causes diabetics painful with dead.
Traditional hypoglycemic medicine is a lot, like insulin, various blood sugar lowering Western medicine, also has naturopathy.The world of medicine generally adopts diabetes treatment by western medicine at present, and toxic and side effects is big, and is difficult to stop the diabetes progress, and various medicines all have its limitation, and is undesirable like post-prandial glycemia control, weight increase, risk of hypoglycemia increase etc.At present to diabetes particularly diabetic complication still do not have gratifying effective ways so far.In the face of this present situation, under the safety prerequisite, improve the important research field that glycemic control becomes treating diabetes and reduces its complication lastingly.
Summary of the invention
The object of the present invention is to provide a kind of compound recipe Herba Dendrobii compound for reducing blood suger and preparation method thereof; The effect that this compound recipe Herba Dendrobii compound for reducing blood suger has mediator's body immunity function, blood glucose and blood pressure level; Improve diabetic symptom, the hold concurrently diabetes and the vascular complication thereof of blood stasis pattern of syndrome of deficiency of both QI and YIN had positive effect.
The objective of the invention is to realize through following technical scheme:
The present invention's compound recipe Herba Dendrobii compound for reducing blood suger is processed by following Chinese medicinal raw materials in proportion by weight medicine: Herba Dendrobii 2g-40g, Radix Rehmanniae Preparata 5g-80g, Fructus Lycii 5g-80g, Fructus Corni 5g-80g, Semen Cuscutae 5g-80g, Radix Astragali 5g-80g, Cortex Lycii 5g-80g, Rhizoma Coptidis 2g-40g, Rhizoma Atractylodis 5g-80g, Galla Chinensis 5g-80g, Cortex Moutan 5g-80g, Radix Glycyrrhizae 2g-40g.
Its preferred weight proportioning is: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.
The present invention's compound recipe Herba Dendrobii compound for reducing blood suger can be on the basis of said component; Add known adjuvants such as lactose, sucrose, dextrin, soluble starch, Mel, Pulvis Talci, magnesium stearate; Be processed into preparations such as granule, tablet, pill, capsule, oral liquid, powder, medicated wine or unguentum respectively, its mass percent is following:
Herba Dendrobii 2g-40g, Radix Rehmanniae Preparata 5g-80g, Fructus Lycii 5g-80g, Fructus Corni 5g-80g, Semen Cuscutae 5g-80g, Radix Astragali 5g-80g, Cortex Lycii 5g-80g, Rhizoma Coptidis 2g-40g, Rhizoma Atractylodis 5g-80g, Galla Chinensis 5g-80g, Cortex Moutan 5g-80g, Radix Glycyrrhizae 2g-40g, adjuvant 10g-100g.
Crude drug Herba Dendrobii in the present invention's the compound recipe Herba Dendrobii compound for reducing blood suger belongs to aerial orchid section herbaceous plant, and put down in writing in the Compendium of Material Medica of Ming Dynasty's Li Shizhen (1518-1593 A.D.): it has the asthenia of the eliminating impediment therapeutic method to keep the adverse QI flowing downwards, tonifying five ZANG-organs, the smart effect of reinforcing YIN-essence benefit.Obey thick the intestines and stomach, exhausted gas in mending for a long time; Come gastric qi, longue meat, Fructus Alpiniae Oxyphyllae removes frightened, makes light of one's life by commiting suicide and prolongs life.The present age, chemistry and pharmacological research were found, contained abundant polysaccharide component in the Herba Dendrobii, had the T of growth cell, B cell, NK cell and macrophage function effect.Polysaccharide component in the Herba Dendrobii has the effect of obvious enhancing T cellular immune function to cancer patient; Help improving the immunity of human body; With the invasion and attack of defending the extraneous source of disease factor with remove the old and feeble casualties, mutant cell in the body, so as to keeping the physiology steady statue of body.
In compound recipe Herba Dendrobii compound for reducing blood suger of the present invention, the Herba Dendrobii reinforcing stomach reg fluid, nourishing YIN and clearing away heat, Radix Rehmanniae Preparata nourishes kidney yin, and the two is monarch drug altogether; Fructus Corni, Fructus Lycii are ministerial drug, close principal agent to strengthen the nourishing kidney-yin effect; Assistant Semen Cuscutae, Radix Astragali benefiting qi and supporting yang, treating YIN within YANG; Rhizoma Coptidis, Cortex Lycii clearing stomach blood sugar lowering; The Cortex Moutan blood circulation promoting and blood stasis dispelling; The Galla Chinensis reducing urination; Rhizoma Atractylodis, Radix Glycyrrhizae and middle spleen invigorating, all medicines share, and play Replenishing QI and nourishing YIN altogether, and blood sugar lowering is invigorated blood circulation, to reach the merit of equilibrium between yin and yang.
The present invention's compound recipe Herba Dendrobii compound for reducing blood suger method for preparing may further comprise the steps:
(1) take by weighing Herba Dendrobii, Semen Cuscutae, Fructus Lycii, Cortex Lycii, Galla Chinensis by said weight ratio, independent or mixed powder is broken into powder (breaking method can be that standard machinery is pulverized, and crosses 200 mesh sieves, also can be crushed to micronization with super micron mill), and is subsequent use;
(2) take by weighing Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Coptidis, Rhizoma Atractylodis, the Radix Astragali, Cortex Moutan, Radix Glycyrrhizae by said weight ratio, be cut into decoction pieces or break into coarse powder, mix; Add and be equivalent to the doubly water of (preferred 8 times) of said crude drug weight 7-9; Lixiviate 110-130 minute, filter, get extracting solution (i.e. filtrating); In filtering residue, add again and be equivalent to the doubly water of (preferred 6 times) of said crude drug weight 5-7, lixiviate 80-100 minute, filter, get extracting solution (i.e. filtrating), merge extracted twice liquid; Again extracting solution is evaporated to the concentrated solution that relative proportion is 1.0-1.2 (preferred 1.10), subsequent use;
(3) be prepared into tablet, capsule, pill, granule, powder, oral liquid, medicated wine or unguentum etc. according to common process:
1. tablet, capsule, pill preparation: get the concentrated solution spraying drying powder-forming that step (2) makes, the powder that makes with step (1) again, mix homogeneously adds known corresponding adjuvant, according to corresponding dosage form common process processing;
2. granule, powder preparation: get the concentrated solution spray drying that step (2) makes, get the micropowder that step (1) makes again, mix homogeneously adds known corresponding adjuvant, according to corresponding dosage form common process processing;
3. the medicated powder that step (1) makes is got in oral liquid, medicated wine, unguentum preparation, adds water or ethanol floods or percolation or decoction, concentrate according to common process, and mixes, stir with the concentrated solution that step (2) makes again, the adding adjuvant, mixing.
The present invention's compound recipe Herba Dendrobii compound for reducing blood suger can improve the human body immunity, materials such as blood, essence, body fluid in the abundant added body, mediator's yin-yang; Significantly the blood sugar lowering level with improve diabetic symptom; Memory reinforcing, the asthenia of tonifying five ZANG-organs; Defying age; Suppress tumor; Anti-hypoxia; The hold concurrently diabetes and the vascular complication thereof of blood stasis pattern of syndrome of deficiency of both QI and YIN had positive effect; Be applicable to diabetes complicated coronary heart disease, cerebrovascular and lower limb vascular, retinopathy; Multiple pathological changes such as diabetic nephropathy, the may command diabetes develop, improve the quality of living; And it is without any side effects.
The specific embodiment
Below in conjunction with embodiment the present invention is described further, but these embodiment must not be used to explain the restriction to protection domain of the present invention.
Embodiment 1
The agent of present embodiment Herba Dendrobii compound granular, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Preparation: (1) takes by weighing Herba Dendrobii, Semen Cuscutae, Fructus Lycii, Cortex Lycii, Galla Chinensis by said weight proportion, is crushed to micronization with super micron mill, and is subsequent use; (2) take by weighing Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Coptidis, Rhizoma Atractylodis, the Radix Astragali, Cortex Moutan, Radix Glycyrrhizae by said weight proportion and be cut into decoction pieces, add the water that is equivalent to 8 times of said crude drug weight, lixiviate 120 minutes is filtered, extracting solution; Add the water that is equivalent to 6 times of said crude drug weight again, lixiviate 90 minutes is filtered, and gets extracting solution, merges extracted twice liquid; Again extracting solution being evaporated to relative proportion is 1.10 concentrated solution, subsequent use; (3), get the extractum fine powder with the concentrated solution spray drying; Sucrose is drying and crushing under 60 ℃ of temperature, crosses 80 mesh sieves; Get micropowder and sucrose powder that extractum fine powder and step (1) make, mix homogeneously adds the ethanol of the mixed material weight 5% be equivalent to said extractum fine powder, micropowder that step (1) makes and cane sugar powder with spray pattern; Granulation, drying, 20 order granulate obtain granule; Packing.
Instructions about how to take medicine, consumption: the agent of this Herba Dendrobii compound granular, the adult takes three times every day, each 2g.
After the agent of this Herba Dendrobii hypoglycemic granule took one month for 10 obvious persons of diabetes polyuria polydipsia symptom, symptom obviously disappeared, and health status is clearly better;
After the agent of this Herba Dendrobii hypoglycemic granule took one month for 10 diabetes muscle power and the obvious person of weight loss symptom, body constitution strengthened, and health status is clearly better.
The agent of this Herba Dendrobii hypoglycemic granule give 10 obvious persons of diabetes visual deterioration symptom take two each and every one month after, vision is recovered basically.
After the agent of this Herba Dendrobii hypoglycemic granule takes one month for the nervous obvious person of constipation symptom that breathes hard, suffers from diarrhoea of 10 diabetes, breathes smooth and easy, diarrhoea constipation transference cure, health status improvement.
The agent of this Herba Dendrobii hypoglycemic granule takes after two months for 10 diabetic urine retentions and the obvious person of sexual impotence symptom, and symptom obviously alleviates, and health status takes a turn for the better.
The agent of this Herba Dendrobii hypoglycemic granule takes after two months for 10 diabetic complication nephrotics, and renal function is clearly better.
After the agent of this Herba Dendrobii hypoglycemic granule took one month for 10 diabetic complication hyperpietics, blood pressure obviously descended, and health status takes a turn for the better.
After the agent of this Herba Dendrobii hypoglycemic granule took one month for 10 diabetic complication cardiovascular and cerebrovascular vessel patients, symptom significantly alleviated, and health status takes a turn for the better.
After the agent of this Herba Dendrobii hypoglycemic granule took one month for 10 patient with diabetic feet, it is normal that numb limbs and tense tendons recovers, and health status takes a turn for the better.
Embodiment 2
Herba Dendrobii compound granular of the present invention agent, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 10g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 15g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Method for preparing is with embodiment 1.
Embodiment 3
Herba Dendrobii compound decoction of the present invention, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Preparation: take by weighing the said raw material of Chinese medicine of respectively distinguishing the flavor of, total amount is 157g, adds 8 times of water gagings, soaks half an hour, be heated to boil after, decocted extracting juice one hour; In medicinal residues, add 6 times of water gagings again, be heated to and boil, decoct half an hour, extracting juice; Merge first and second medicine juice, stir, promptly get decoction.
Instructions about how to take medicine, consumption: said decoction divides obeyed in three times one day.
Embodiment 4
Herba Dendrobii compound decoction of the present invention, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 10g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 15g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Method for preparing is with embodiment 3.
Embodiment 5
Herba Dendrobii compound recipe unguentum of the present invention, its crude drug compound recipe composition proportion is:
Major ingredient: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Preparation: (1) takes by weighing Herba Dendrobii, Semen Cuscutae, Fructus Lycii, Cortex Lycii, Galla Chinensis pulverizing by said weight proportion, crosses 200 mesh sieves; In medicated powder, add and be equivalent to 8 times of water gagings of said crude drug weight, decoct, filter, merging filtrate is evaporated to relative proportion 1.10, and is subsequent use; (2) take by weighing Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Coptidis, Rhizoma Atractylodis, the Radix Astragali, Cortex Moutan, Radix Glycyrrhizae by said weight proportion, be cut into decoction pieces, add the water that is equivalent to 8 times of said crude drug weight, lixiviate 120 minutes is filtered, and gets extracting solution; Add the water that is equivalent to 6 times of said crude drug weight again, lixiviate 90 minutes is filtered, and gets extracting solution, merges extracted twice liquid; Again extracting solution being evaporated to relative proportion is 1.10 concentrated solution, subsequent use; (3) combining medicine that step (1), (2) is made, mix homogeneously is evaporated to relative proportion 1.45, adds Mel, sucrose, stirs, and it is 1.50 extractum that reheat is concentrated into relative proportion, sterilization, canned, packing.
Embodiment 6
Herba Dendrobii compound recipe unguentum of the present invention, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 10g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 15g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Mel 30 g, sucrose 10g.
Method for preparing is with embodiment 5.
Embodiment 7
Herba Dendrobii compound capsule of the present invention agent, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Pulvis Talci 0.3g, magnesium stearate 0.3g.
Preparation: (1) takes by weighing Herba Dendrobii, Semen Cuscutae, Fructus Lycii, Cortex Lycii, Galla Chinensis by said weight proportion and is crushed to micronization with super micron mill, and is subsequent use; (2) take by weighing Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Coptidis, Rhizoma Atractylodis, the Radix Astragali, Cortex Moutan, Radix Glycyrrhizae by said weight proportion, be cut into decoction pieces, add the water that is equivalent to 8 times of said crude drug weight, lixiviate 120 minutes is filtered, and gets extracting solution; Add the water that is equivalent to 6 times of said crude drug weight again, lixiviate 90 minutes is filtered, and gets extracting solution, merges extracted twice liquid; Again extracting solution being evaporated to relative proportion is 1.10 concentrated solution, subsequent use; (3), get the extractum fine powder with the concentrated solution spray drying; The drug powder mix homogeneously that more said extractum fine powder and step (1) is made adds adjuvant (Pulvis Talci 0.3g, magnesium stearate 0.3g) mix homogeneously, filling capsule, and packing.
Embodiment 8
Herba Dendrobii compound capsule of the present invention agent, its crude drug proportioning is:
Major ingredient: Herba Dendrobii 10g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 15g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.Adjuvant: Pulvis Talci 0.3g, magnesium stearate 0.3g.
Method for preparing is with embodiment 7.
The oral liquid curative effect of embodiment 2-8 and embodiment 1 product curative effect are approaching.
The agent of Herba Dendrobii hypoglycemic granule, embodiment 7 capsules with the embodiment of the invention 1 carry out the safety toxicology test:
Laboratory sample and character: sample is a brown powder, is modulated into each concentration with distilled water, supplies examination.
Laboratory animal: Wistar rat and Kunming kind white mice are provided by drug research institute of Central South University animal center receptacle.
1, acute toxicity testing: with acute toxicity half lethal dose toxicity grading, these article are not measured LD
50(half lethal dose), mice maximum tolerated dose are 200g/kg/ day, observe continuously seven days, and 20 mices all survive, and abnormal response do not occur.Granule of the present invention is given the maximum tolerance of mouse stomach administration multiple 600 times for adult's oral dose every day.
2, MTT cytotoxicity experiment: adopt tetrazolium bromide (MTT) method that granule of the present invention is carried out cytotoxicity analysis, the experiment triplicate is not measured LD
50(half lethal dose), ED
0(maximum no-effect dose)=1ml (medicinal liquid)/10ml (culture fluid).
3, micronucleus test: sample bone marrow polychromatic erythrocyte micronucleus test is the result be negative.
4, Ames (Salmonella typhimurium back mutation experiment) experiment: experimental result is negative.
5, long term toxicity test:
Give the low middle high dose 3g of the rat ig embodiment of the invention 7 capsules, 6g, 12g/kg, be equivalent to 25 times, 50 times and 100 times of clinical adult's dose every day respectively, once a day; Continuous one month, the laboratory animal growing state was good, hematological examination; Blood biochemical is learned inspection; Uroscopy, main dirty body is histological examination result when compare with matched group, all do not have significant difference.Prove that preparation safety of the present invention is nontoxic.
Experimental technique and result:
5.1 growing state and body weight
Divide four groups at random with animal, 10 every group, male and female half and half, design is according to dosage fed after 30 days continuously, weighs.
Each administration group and normal control group rat relatively, the ordinary circumstance Non Apparent Abnormality (movable normal, feed is normal, growth, grow normal).Concrete condition is seen table 1.
Table 1: embodiment 7 products are added up the result of the test that influences of growing state
Through statistical analysis: sample does not have obvious influence to the weight of animals.
5.2 dirty body ratio
1. each the administration group of finding of naked eye and the normal control group rat heart, liver, spleen, lung, kidney, stomach, intestinal are all normal.
2. microscopically is observed each administration group and the normal control group rat heart, liver, spleen, lung, kidney, to liver,spleen,kidney and testis or ovary is weighed and compare with body weight, calculates dirty body ratio.Equal no abnormality seen pathological changes.Concrete condition is seen table 2.
Table 2: embodiment 7 products influence the result of the test statistics to liver,spleen,kidney and testis or ovary
Through statistical analysis: the dirty body of processed group is compared than with matched group, does not all have significant difference.
5.3 hematological examination
In the blood test, RBC (RBC), PLT (platelet count), WBC (numeration of leukocyte), Hb (hemoglobinometry) male and female zero difference are so average.Concrete condition is seen table 3.
Table 3: embodiment 7 products influence the result of the test statistics mutually to blood
Through statistical analysis: the processed group hematological examination is the result compare with matched group, all do not have significant difference.
5.4 blood biochemistry checking
Embodiment 7 products influence result of the test to blood parameters and see table 4.
Table 4: embodiment 7 products influence the result of the test statistics to blood parameters
Through statistical analysis: the processed group blood parameters is compared with matched group, does not all have significant difference.
5.5 routine urianlysis
Embodiment 7 products influence result of the test to the urine index and see table 5.
Table 5: embodiment 7 products influence the result of the test statistics to the urine index
Through statistical analysis: processed group routine urinalysis index is compared with matched group, does not all have significant difference.
Carry out relevant immunomodulating, blood sugar lowering, hypotensive activity test with Herba Dendrobii compound preparation granule of the present invention.
(1) immunoregulation effect test
Experiment establish low in three dose groups of high dose 1.8g, 3.6g, 6.0g/kg/d (be equivalent to respectively clinical adult's dose every day 6g/d 15 times, 30 times and 50 times), establish a matched group simultaneously.
1. the tardy paraphilia reaction test of dinitrofluorobenzene inducing mouse, the result sees table 6.
Table 6: embodiment 7 products are to the influence of mouse immune cell function
* P 0.05, compare with matched group that there were significant differences, < 0.01 has utmost point significant difference to * * P.
2. serum hemolysin is measured, and the result sees table 7.
Table 7: embodiment 7 products are to the influence of mouse humoral immune function
* P 0.05, compare with matched group that there were significant differences, < 0.01 has utmost point significant difference to * * P.
3. mononuclear phagocyte functional experiment, the result sees table 8.
Table 8: embodiment 7 products are to the influence of mononuclear-macrophage phagocytic function
* P 0.05, compare with matched group that there were significant differences.
Visible by table 8, each dose groups mouse monokaryon macrophage phagocytic index all is higher than matched group, and low dose group has notable statistics difference (P < 0.05).
The tardy paraphilia reaction and display of dinitrofluorobenzene inducing mouse Herba Dendrobii Jiangtang capsule of the present invention can significantly increase the mouse immune cell function in this experiment; Serum hemolysin is measured and is shown that Herba Dendrobii Jiangtang capsule of the present invention can significantly increase the mouse humoral immune function; The clearance test of mice carbon shows that the Herba Dendrobii Jiangtang capsule can significantly increase mouse monokaryon-macrophage phagocytic function.Experimental result shows that Herba Dendrobii Jiangtang capsule of the present invention has immunoregulation effect preferably.
(2) hypoglycemic activity test
The Herba Dendrobii Jiangtang capsule is to the influence of normal mouse blood sugar and serum insulin
Divide into groups and medication: 40 of mices are divided into four groups at random; Every group 10, wherein matched group is given the equivalent normal saline, and test group is used a low middle Senior Three dosage 1.8,3.6,6.0 g/kg/d of Herba Dendrobii Jiangtang capsule respectively; Each group all adopts ig administration, successive administration 7d.The 2h broken end is got blood after the last administration, uses the determination of glucose oxidase blood glucose value, uses the measured by radioimmunoassay insulin.The result sees table 9.
Table 9: embodiment 7 products are to the influence of normal mouse blood sugar and serum insulin
The result shows, normal mouse Herba Dendrobii Jiangtang capsule 1.8,3.6,6.0 g/kg/d, and blood glucose and insulin change not obvious behind the ig.
The Herba Dendrobii Jiangtang capsule is to the influence of adrenal gland's disposition hyperglycemia mouse blood sugar and hepatic glycogen content.
Divide into groups and medication: 50 of mices are divided into four groups at random; Every group 10, wherein matched group and epinephrine matched group are given the equivalent normal saline, and test group is used a low middle Senior Three dosage 1.8,3.6,6.0 g/kg/d of Herba Dendrobii Jiangtang capsule respectively; Each group all adopts ig administration, successive administration 7d.70min after the last administration, administration group and adrenal gland's matched group are all with epinephrine 0.2mg/kg, and sc breaks end behind the 50min and gets blood survey blood glucose, and gets liver rapidly, measures hepatic glycogen content with the iodine reagent method.The result sees table 10.
Table 10: embodiment 7 products are to the influence of adrenal gland's disposition hyperglycemia mouse blood sugar and hepatic glycogen content
* P 0.05, compare with the epinephrine matched group that there were significant differences, < 0.01 has compared utmost point significant difference with the epinephrine matched group to * * P.## P < 0.01 utmost point significant difference of having compared with normal control.
Experimental result shows that blood glucose value obviously raises behind the mice sc epinephrine, and hepatic glycogen content obviously reduces, and Herba Dendrobii Jiangtang capsule 1.8,3.6,6.0 g/kg can make the blood glucose value of adrenal gland's disposition hyperglycemia mice obviously reduce, and hepatic glycogen content obviously increases.
(3) hypotensive activity test
40 of grouping and medication: SD
property kidney type high pressure rats; Body weight 180-220g; Be divided into four groups by pressure value, 10 every group, make every group of mediodespidine average basically identical; Wherein matched group is given the equivalent normal saline; Its excess-three group is used a low middle Senior Three dosage 1.8,3.6,6.0 g/kg/d of Herba Dendrobii compound preparation respectively, and each group all adopts the ig administration, decides blood pressure with the rat blood pressure instrumentation behind the successive administration 10d.The result sees table 11.
Table 11: embodiment 7 products are to the influence of rat blood pressure
* P 0.05, compare with matched group that there were significant differences.
Experimental result shows that embodiment 7 products have certain hypotensive effect to kidney type high pressure rat.
Herba Dendrobii Jiangtang capsule of the present invention; Taking for a long time can mediator's body immunity function, blood sugar regulation and blood pressure level usefulness, and can improve diabetic symptom; The hold concurrently diabetes and the vascular complication thereof of blood stasis pattern of syndrome of deficiency of both QI and YIN had positive effect; Be applicable to diabetes complicated coronary heart disease, cerebrovascular and lower limb vascular, retinopathy, multiple pathological changes such as diabetic nephropathy, the may command diabetes develop, improve the quality of living.Without any side effects.Through clinical trial, total effective rate reaches 100%, and concrete result of the test is reported as follows:
One, subjects
Obvious person's 10 examples of diabetes polyuria polydipsia symptom; Diabetes muscle power and obvious person's 10 examples of weight loss symptom; Obvious person's 10 examples of diabetes visual deterioration symptom; Nervous obvious person's 10 examples of constipation symptom of breathing hard, suffer from diarrhoea of diabetes; Obvious person's 10 examples of diabetic urine retention and sexual impotence symptom; Diabetic complication nephrotic 10 examples; Diabetic complication hyperpietic 10 examples; Diabetic complication cardiovascular and cerebrovascular vessel patient 10 examples; Patient with diabetic feet 10 examples.
Two, usage and consumption
Oral Herba Dendrobii capsule of the present invention, every day 3 times (rise morning once, before lunch, the dinner each once), 2g/ time, a week is a course of treatment, serve on 4 courses of treatment; For consolidating curative effect, health invigorating was withdrawn after two weeks, added 2 courses of treatment again.
Three, curative effect judgment standard
The doing well,improving degree: the improvement degree is divided into produce effects, effective, invalid.
Produce effects: 100% original transference cure.
Effectively: original transference cure or alleviate more than 60%.
Invalid: original sx below 60% or do not have fully alleviates.
Four, clinical observation result
Total effective rate 100%, as shown in the table:
Claims (4)
1. compound recipe Herba Dendrobii compound for reducing blood suger; It is characterized in that, process: Herba Dendrobii 2g-40g, Radix Rehmanniae Preparata 5g-80g, Fructus Lycii 5g-80g, Fructus Corni 5g-80g, Semen Cuscutae 5g-80g, Radix Astragali 5g-80g, Cortex Lycii 5g-80g, Rhizoma Coptidis 2g-40g, Rhizoma Atractylodis 5g-80g, Galla Chinensis 5g-80g, Cortex Moutan 5g-80g, Radix Glycyrrhizae 2g-40g by the raw material of Chinese medicine medicine of following weight.
2. compound recipe Herba Dendrobii compound for reducing blood suger according to claim 1; It is characterized in that each raw material of Chinese medicine medicine composition weight is: Herba Dendrobii 6g, Radix Rehmanniae Preparata 10g, Fructus Lycii 10g, Fructus Corni 10g, Semen Cuscutae 10g, Radix Astragali 20g, Cortex Lycii 10g, Rhizoma Coptidis 5g, Rhizoma Atractylodis 10g, Galla Chinensis 10g, Cortex Moutan 10g, Radix Glycyrrhizae 6g.
3. the method for preparing of compound recipe Herba Dendrobii compound for reducing blood suger according to claim 1 or claim 2 is characterized in that, may further comprise the steps:
(1) take by weighing Herba Dendrobii, Semen Cuscutae, Fructus Lycii, Cortex Lycii, Galla Chinensis by said weight ratio, independent or mixed powder is broken into powder, and is subsequent use;
(2) take by weighing Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Coptidis, Rhizoma Atractylodis, the Radix Astragali, Cortex Moutan, Radix Glycyrrhizae by said weight ratio, be cut into decoction pieces or break into coarse powder, mix, add and be equivalent to said crude drug weight 7-9 water doubly, lixiviate 110-130 minute, filter, get extracting solution; In filtering residue, add again and be equivalent to said crude drug weight 5-7 water doubly, lixiviate 80-100 minute, filter, get extracting solution, merge extracted twice liquid; Again extracting solution is evaporated to the concentrated solution that relative proportion is 1.0-1.2, subsequent use;
(3) be prepared into tablet, capsule, pill, granule, powder, oral liquid, medicated wine or unguentum according to common process:
1. tablet, capsule, pill preparation: get the concentrated solution spraying drying powder-forming that step (2) makes, the powder that makes with step (1) again, mix homogeneously adds known corresponding adjuvant, according to corresponding dosage form common process processing;
2. granule, powder preparation: get the concentrated solution spray drying that step (2) makes, get the micropowder that step (1) makes again, mix homogeneously adds known corresponding adjuvant, according to corresponding dosage form common process processing;
3. the medicated powder that step (1) makes is got in oral liquid, medicated wine, unguentum preparation, adds water or ethanol floods or percolation or decoction, concentrate according to common process, and mixes, stir with the concentrated solution that step (2) makes again, the adding adjuvant, mixing.
4. according to the method for preparing of the said compound recipe Herba Dendrobii of claim 3 compound for reducing blood suger; It is characterized in that; Step (2); The amount of water of lixiviate for the first time is equivalent to 8 times of said crude drug weight, and the lixiviate of lixiviate for the second time amount of water is equivalent to 6 times of said crude drug weight, and it is 1.10 that extracting solution is evaporated to relative proportion.
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| CN102641430B (en) * | 2012-05-05 | 2013-09-11 | 刘健 | Traditional Chinese medicine decoction for treating diabetic nephropathy (DN) |
| CN103784882A (en) * | 2014-02-18 | 2014-05-14 | 吕志强 | Compound traditional Chinese medicine for treating diabetic nephropathy and preparation method thereof |
| EP3151843B1 (en) | 2015-04-29 | 2020-07-15 | Infinitus (China) Company Ltd. | Compositions comprising cyclocarya paliurus extract and preparation method and uses thereof |
| CN106256267A (en) * | 2015-06-15 | 2016-12-28 | 王林华 | A kind of Herba Dendrobii is the new product formula of raw material |
| CN105687771A (en) * | 2016-02-18 | 2016-06-22 | 云南久丽康源石斛开发有限公司 | Herba dendrobii capsule with auxiliary blood glucose lowering function and method for preparing herba dendrobii capsule |
| CN109200193A (en) * | 2018-09-27 | 2019-01-15 | 商丘医学高等专科学校 | Tiepi Fengdou is to diabetic nephropathy and mesangial cell signal detecting method |
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| CN101391033A (en) * | 2008-10-23 | 2009-03-25 | 王湛 | Internal medicine for treating diabetes |
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