CN102038714A - Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus - Google Patents
Application of bdellovibrio bdelloplast in preparing bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus Download PDFInfo
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- CN102038714A CN102038714A CN2010102686332A CN201010268633A CN102038714A CN 102038714 A CN102038714 A CN 102038714A CN 2010102686332 A CN2010102686332 A CN 2010102686332A CN 201010268633 A CN201010268633 A CN 201010268633A CN 102038714 A CN102038714 A CN 102038714A
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Abstract
The invention discloses application of bdellovibrio bdelloplast in preparing a bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus. The bdellovibrio is (Bdellovibrio sp.) BDM01. The application method for the bacterial agent comprises that: the bacterial agent is put into water body to prevent and control the red mouth disease of scophthalmus maximus to ensure that the final concentration of the bdellovibrio bdelloplast in the water body is 10-10<7>pfu/mL, or a feed is soaked by the bacterial agent for controlling the red mouth disease of scophthalmus maximus for 15 to 45 minutes, and the concentration of the bdellovibrio bdelloplast in the bacterial agent is 10-10<7>pfu/mL. When the bdellovibrio bdelloplast is applied to preparing the bacterial agent for preventing and controlling the red mouth disease of scophthalmus maximus, the bacterial agent can effectively prevent and control the red mouth disease of scophthalmus maximus, has high safety when applied to aquaculture, is environment-friendly, does not cause pathogenic bacteria resistance, and has good application prospect.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of application of mushroom, particularly Bdellovibrio trematodiasis plastid is prevented and treated application in the agent of the red mouth pathogenic bacteria of turbot in preparation.
Background technology
Turbot (Scophthalmus maximus) is a kind of famous and precious Flounder Pleuronectidae class that originates in Europe.Because having, it adapts to low water temperature life, fast growth, and delicious meat, acceptant man-made feeds are easy to advantages such as intensive culture, after the introduction China in 1992, become China's coastal important breed variety in the north rapidly.Yet overrun development and the running lack of standardization of breed have caused the disease problem outstanding day by day, the multiple manifest symptom and the extensive phenomena of mortality have occurred, and red mouth disease is exactly wherein a kind of, has caused heavy economic loss for the breed dealer.The cardinal symptom of red mouth disease shows as: the sick fish kiss end and the outside of belly upper jaw are hemorrhage, the part fish gill cover has the hemorrhage little pothole that festers, the oral cavity internal hemorrhage, the enlargement of the sick flake eyeball of part is given prominence to, needled a large amount of puss in the adventitia, the congested no food of intestinal, Di has a small amount of thick liquid, and liver hyperemia, color dimness are the rotten shape that looses, and the spleen enlargement is rubescent.In therapeutic process, the raiser is the economic pursuit benefit, and phenomenon such as medication happens occasionally carelessly, causes the pesticide residues problem to be on the rise.At present, prevent that the main method of disease from being the sterilizing medicine that adopts, but itself exist many shortcomings that as drug resistance and drug residue that antibiotic use causes, the residual incident of turbot nitrofurans medicine that took place in 2006 is just very representative.Therefore, how to carry out healthy aquaculture, Aquatic product circle that just becomes problem demanding prompt solution.
Bdellovibrio is to parasitize other antibacterials, and can cause the cracked bacterioid of host.Littler than general antibacterial, can pass through bacterial filter, the effect of similar phage is arranged.Unicellular, curved or funny point-like, shape in the shape of a spiral sometimes, size (0.2-0.6) * (0.8-1.2) micron, end is given birth to flagellum, mostly is one.Gram is negative, and cleavable causes a disease or potential pathogenic bacteria.The life cycle of Bdellovibrio divides two stages: free living, can move telotroch stage of not breeding and the trematodiasis plastid stage of in the periplasmic space of specific host antibacterial, carrying out growth and breeding.The Bdellovibrio telotroch is meant the growth forms before Bdellovibrio is invaded host bacteria.Bdellovibrio is in the telotroch stage, and identification also with the host irreversible fixation takes place, and invades the host then, and it is very fast that the Bdellovibrio telotroch is invaded the process of host bacteria, can finish in general several seconds.Bdellovibrio trematodiasis plastid then is Bdellovibrio carries out growth and breeding in the periplasmic space of specific host antibacterial a form.After Bdellovibrio entered host's bacterial cell periplasmic space, mainly the macromole by the degraded host cell became monomer, took in then to be used for the synthetic of its biomacromolecule.Along with cell proliferation and hydrolytic enzyme produce, make the host cell wall destroyed, thereby cracking discharge Bdellovibrio.
Bdellovibrio trematodiasis plastid is Bdellovibrio carries out growth and breeding in the periplasmic space of specific host antibacterial a form.Compare with telotroch, the trematodiasis plastid has preparation, preservation is more convenient, and the environment tolerance is strong, the characteristics though the bactericidal action time slightly more waits so long slowly.These characteristics make Bdellovibrio trematodiasis plastid be suitable as the biological cleaning factor of restraining or removing pathogenic bacterium in organism and the environment thereof equally, thereby have overcome the drug residue problem that the antibiotic abuse causes.At present, prevent with Bdellovibrio trematodiasis plastid and the method for the treatment of red mouth disease equal report at home and abroad.
Summary of the invention
In order to overcome the weak point that abuse of antibiotics in the fish culture causes various food-safety problems, the invention provides a kind of Bdellovibrio trematodiasis plastid and prevent and treat application in the agent of the red mouth pathogenic bacteria of turbot in preparation.
The present invention is achieved through the following technical solutions:
Bdellovibrio trematodiasis plastid is prevented and treated application in the agent of the red mouth pathogenic bacteria of turbot in preparation.
The preparation method of described Bdellovibrio trematodiasis plastid is: the host is in the nutrient broth fluid medium in inoculation, at 35 ℃ and 200rpm shaking table cultivation 12h, culture fluid is through 4 ℃, behind the centrifugal 10～20min of 5000～8000rpm, precipitation is added in the DNB fluid medium, insert Bdellovibrio BDM01, under 25～35 ℃ of temperature, cultivate 36～48h with 150～300rpm shaking table, obtain containing the culture fluid of Bdellovibrio trematodiasis plastid and Bdellovibrio telotroch, culture fluid behind centrifugal 15～20min, is removed the supernatant that contains the Bdellovibrio telotroch with 6000～8000rpm under 4 ℃ of temperature, the precipitation that obtains is Bdellovibrio trematodiasis plastid; Is that 7.2～7.6 phosphate buffer suspends with Bdellovibrio trematodiasis plastid with DNB fluid medium, water, normal saline or 0.2mol/L pH value, and obtaining concentration is 10
6～10
9The Bdellovibrio trematodiasis plastid bacterium liquid of pfu/mL.
Described Bdellovibrio was Bdellovibrio (Bdellovibrio sp.Strain) BDM01, was preserved in Chinese typical culture collection center on April 28th, 2008, and the preservation address is Chinese Wuhan Wuhan University, and deposit number is CCTCC NO:M 208066.
Described Bdellovibrio trematodiasis plastid is to splash to prevent and treat the agent of the red mouth pathogenic bacteria of turbot in water body in the application that preparation prevents and treats in the agent of the red mouth pathogenic bacteria of turbot, changes the microbial inoculum of evenly splashing in water body after the water at every turn, makes that Bdellovibrio trematodiasis plastid final concentration is 10-10 in the water body
7Pfu/mL.
Described Bdellovibrio trematodiasis plastid is that feedstuff is soaked 15～45min with preventing and treating the agent of the red mouth pathogenic bacteria of turbot in the application that preparation prevents and treats in the agent of the red mouth pathogenic bacteria of turbot, and Bdellovibrio trematodiasis plastid concentration is 10-10 in the microbial inoculum
7Pfu/mL throws feedstuff every day three times, and early, middle and late respectively once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.4% of the mass fraction of daily ration of feeding and fish average weight perfuses full pond.
The present invention has following advantage and effect with respect to prior art:
1, Bdellovibrio trematodiasis plastid of the present invention can effectively prevent and treat the red mouth disease of turbot in the application that preparation prevents and treats in the agent of the red mouth pathogenic bacteria of turbot.
As show shown in the 1-2, the application of the trematodiasis of Bdellovibrio described in the present invention plastid in the sick control of the red mouth of turbot can effectively reduce the probability that turbot is suffered from red mouth disease, and effect is remarkable on the red mouth disease of treatment, compare with the antibiotic method of the use of routine, it is a kind of method of environmental protection.
The red mouth disease of turbot is a kind of bacterial disease, and bacterial disease is a The main pathogenic fungi with some strain in Aeromonas (Aeromonas), Rhodopseudomonas (Pseudoumonas), the vibrio (Vibrio).Bdellovibrio has the effect of class phage, and it has splitting action to the Fish common pathogen, can play the effect of eliminating the water body pathogenic bacterium.In addition, Bdellovibrio also has certain Degradation to the ammonia nitrogen in the water, can purifying water body, enhance the transparency, and the control fish disease there is certain effect.
2, to prevent and treat in the application that is applied in aquaculture of aquatic animal in the agent of the red mouth pathogenic bacteria of turbot safety in preparation good for Bdellovibrio trematodiasis plastid of the present invention.
3, Bdellovibrio trematodiasis plastid of the present invention preparation prevent and treat in the agent of the red mouth pathogenic bacteria of turbot application effectively, environmental protection, do not produce the pathogen resistance, application prospect is good.
Concrete implementing measure
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
The preparation of embodiment 1 microbial inoculum
The Bdellovibrio bacteriovorus bacterial strain that uses is Bdellovibrio BDM01.Described Bdellovibrio (Bdellovibriosp.) BDM01 is preserved in Chinese typical culture collection center on April 28th, 2008, and deposit number is CCTCC NO:M 208066; It is carried out morphologic observation under ultramicroscope after the negative staining: Bdellovibrio BDM01 is unicellular, arc, and size is 1.7 * 1.0 μ m, and end is given birth to flagellum, and flagellum length is 3.5 μ m; It is cultivated the transparent circular plaque that can form diameter 3-4mm in three days with the double-layer plate method in 28 ℃.
Be equipped with the common nutrient broth of 100mL (NB) culture medium (peptone 10g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, distilled water 1000mL, inoculation 1mL concentration is 10 in conical flask pH7.4)
9The Aeromonas hydrophila of cfu/mL (Aeromonas hydrophila purchases in Guangdong Province DSMZ of Institute of Micro-biology, numbering GIM1.172), 200rpm, 28 ℃ of shaking tables were cultivated 18 hours, and culture fluid is abandoned supernatant respectively at 4 ℃, the centrifugal 15min of 5000rpm.Precipitation joined 100mL DNB fluid medium is housed (yeast extract 0.1g is dissolved in the 1000mL distilled water for nutrient broth 0.8g, caseinic acid hydrolysate 0.5g, and pH value is that adding 1mL concentration again is 10 in 7.4 the conical flask
3The plaque of the Bdellovibrio BDM01 of pfu/mL.Constant temperature shaking table 250rpm, 28 ℃ of cultivation 36h.Culture fluid is removed the supernatant that contains the Bdellovibrio telotroch in 4 ℃ of centrifugal 20min of 6000rpm, keeps precipitation (being Bdellovibrio trematodiasis plastid), adds the 1mL normal saline and suspends, and can obtain concentration is 10
8The Bdellovibrio trematodiasis plastid microbial inoculum of pfu/mL.
Embodiment 2
Select physical health, individual juvenile fish of uniform size, average weight is 55 ± 2.5g, according to 300 tails/m
2Cultivation density drop in the pond, be 4 months each experimental period of culturing the pond.
Embodiment 2.1
The outer draining of normal flow hydromining, whenever quantity of exchanged water is 8 streamflows round the clock, throws in conventional turbot pellet (breeding way of plant produced), not with microbial inoculum.
Embodiment 2.2
Changed water one time in per 40 days, and threw in conventional turbot pellet, not with microbial inoculum.
Embodiment 2.3
The embodiment 1 that splashes in water obtains microbial inoculum, makes that Bdellovibrio trematodiasis plastid concentration reaches 10pfu/mL in the water body, and the conventional turbot pellet of feeding adds bacterium after changing a water in per 40 days.
Embodiment 2.4
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 102pfu/mL in the water body, and the conventional turbot pellet of feeding adds bacterium after changing a water in per 40 days.
Embodiment 2.5
The microbial inoculum that feeding obtains through embodiment 1 soaks the conventional turbot pellet of 30min, and Bdellovibrio trematodiasis plastid concentration reaches 10pfu/mL in the used microbial inoculum, bait throwing in every day three times, early, middle and late each (7:00 once, 12:00,17:00), the process of at every turn throwing something and feeding continues 30min.The mass fraction of daily ration of feeding and fish average weight is 4%, perfuses full pond, changes one time water in per 40 days.
Embodiment 2.6
The microbial inoculum that feeding obtains through embodiment 1 soaks the feedstuff of 30min, and Bdellovibrio trematodiasis plastid concentration reaches 10 in the used microbial inoculum
2Pfu/mL, bait throwing in every day three times, it is early, middle and late that respectively once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.The mass fraction of daily ration of feeding and fish average weight is 4%, perfuses full pond, changes one time water in per 40 days.
Embodiment 2.7
The microbial inoculum that the embodiment 1 that splashes in water obtains, make that Bdellovibrio trematodiasis plastid concentration reaches 10pfu/mL in the water body, changed in per 40 days behind water with microbial inoculum, the microbial inoculum that the while feeding obtains through embodiment 1 soaks the conventional turbot pellet of 30min, bait throwing in every day three times, early, middle and late each once (7:00,12:00,17:00), the process of at every turn throwing something and feeding continues 30min.The mass fraction of daily ration of feeding and fish average weight is 4%, perfuses full pond, and Bdellovibrio trematodiasis plastid concentration reaches 10pfu/mL in the used microbial inoculum.
Embodiment 2.8
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
2Pfu/mL adds bacterium after changing a water in per 40 days, and feeding soaks the conventional turbot pellet of 30min through Bdellovibrio trematodiasis plastid simultaneously, bait throwing in every day three times, early, middle and late each once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.Bdellovibrio trematodiasis plastid concentration reaches 10 in the used microbial inoculum
2Pfu/mL.
Experimental result
The sick incidence rate of the red mouth of table 1.
Result of the test shows, under normal factory breeding way, the incidence rate of red mouth disease is about 10%, adopts behind the Bdellovibrio trematodiasis plastid the sick incidence rate of red mouth to have significantly and descends, and minimum is 1.10%.Changing under the regimen condition of same frequency, the sick incidence rate of the red mouth of blank nearly 21%, and red mouth disease does not appear after having used Bdellovibrio trematodiasis plastid, illustrate that Bdellovibrio trematodiasis plastid can effectively prevent the generation of red mouth disease.
Embodiment 3
Select that the turbot of just suffering from red mouth disease is characterized as fish kiss end and the outside of belly upper jaw is hemorrhage, the fish gill cover has the hemorrhage little pothole that festers, and the eyes enlargement is outstanding, culture in capacity is 1 ton breed pond, each cultures pond 150 tails, and test period is 7 days, writes down disease fish quantity every day.
Embodiment 3.1
For normal flowing water (adopt outer draining, whenever quantity of exchanged water is 8 streamflows round the clock), throw in conventional turbot pellet (breeding way of plant produced)
Embodiment 3.2
For throwing in conventional turbot pellet, do not add Bdellovibrio trematodiasis plastid, duration of test does not change water.
Embodiment 3.3
Clean for adding sharp bacterium, sharp bacterium mixed only add low amounts of water after in conventional turbot pellet, stirring evenly and make it bonding, directly throw something and feed.The each medication 12g of every 500kg fish.
Embodiment 3.4
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
3Pfu/mL, the conventional turbot pellet of feeding, duration of test does not change water.
Embodiment 3.5
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
5Pfu/mL, the conventional turbot pellet of feeding, duration of test does not change water.
Embodiment 3.6
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
7Pfu/mL, the conventional turbot pellet of feeding, duration of test does not change water.
Embodiment 3.7
The microbial inoculum that feeding obtains through embodiment 1 soaks the conventional turbot pellet of 30min, and used Bdellovibrio trematodiasis plastid concentration reaches 10
3Pfu/mL, bait throwing in every day three times, early, middle and late each once, (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.The mass fraction of daily ration of feeding and fish average weight is 4%, perfuses full pond, and duration of test does not change water.
Embodiment 3.8
The microbial inoculum that feeding obtains through embodiment 1 soaks the conventional turbot pellet of 30min, and used Bdellovibrio trematodiasis plastid concentration reaches 10
5Pfu/mL, bait throwing in every day three times, early, middle and late each once, (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.The mass fraction 4% of daily ration of feeding and fish average weight perfuses full pond, and duration of test does not change water.
Embodiment 3.9
The microbial inoculum that feeding obtains through embodiment 1 soaks the feedstuff of 30min, and used Bdellovibrio trematodiasis plastid concentration reaches 10
7Pfu/mL, bait throwing in every day three times, early, middle and late each once, (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.The mass fraction of daily ration of feeding and fish average weight is 4%, perfuses full pond, and duration of test does not change water.
Embodiment 3.10
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
3Pfu/mL, duration of test do not change water, and simultaneously feeding soaks the conventional turbot pellet of 30min through Bdellovibrio trematodiasis plastid, bait throwing in every day three times, early, middle and late each once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.Used Bdellovibrio trematodiasis plastid concentration reaches 10
3Pfu/mL.
Embodiment 3.11
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
5Pfu/mL, duration of test do not change water, and simultaneously feeding soaks the conventional turbot pellet of 30min through Bdellovibrio trematodiasis plastid, bait throwing in every day three times, early, middle and late each once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.Used Bdellovibrio trematodiasis plastid concentration reaches 10
5Pfu/mL.
Embodiment 3.12
The microbial inoculum that the embodiment 1 that splashes in water obtains makes that Bdellovibrio trematodiasis plastid concentration reaches 10 in the water body
7Pfu/mL, duration of test do not change water, and simultaneously feeding soaks the conventional turbot pellet of 30min through Bdellovibrio trematodiasis plastid, bait throwing in every day three times, early, middle and late each once (17:00), the process of at every turn throwing something and feeding continues 30min for 7:00,12:00.Used Bdellovibrio trematodiasis plastid concentration reaches 10
7Pfu/mL.
Experimental result
Table 2. turbot cure rate (%)
The result shows, uses Bdellovibrio trematodiasis plastid can 100% to cure red mouth disease, and Bdellovibrio trematodiasis plastid concentration is high more, and the sick recovery from illness of red mouth is fast more.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1. Bdellovibrio trematodiasis plastid is prevented and treated application in the agent of the red mouth pathogenic bacteria of turbot in preparation.
2. prevent and treat application in the agent of the red mouth pathogenic bacteria of turbot according to the described Bdellovibrio trematodiasis of claim 1 plastid in preparation, it is characterized in that: the preparation method of described Bdellovibrio trematodiasis plastid is:
The host is in the nutrient broth fluid medium in inoculation, at 35 ℃ and 200rpm shaking table cultivation 12h, culture fluid is through 4 ℃, behind the centrifugal 10～20min of 5000～8000rpm, precipitation is added in the DNB fluid medium, insert Bdellovibrio BDM01, under 25～35 ℃ of temperature, cultivate 36～48h with 150～300rpm shaking table, obtain containing the culture fluid of Bdellovibrio trematodiasis plastid and Bdellovibrio telotroch, culture fluid behind centrifugal 15～20min, is removed the supernatant that contains the Bdellovibrio telotroch with 6000～8000rpm under 4 ℃ of temperature, the precipitation that obtains is Bdellovibrio trematodiasis plastid; Is that 7.2～7.6 phosphate buffer suspends with Bdellovibrio trematodiasis plastid with DNB fluid medium, water, normal saline or 0.2mol/L pH value, and obtaining concentration is 10
6～10
9The Bdellovibrio trematodiasis plastid of pfu/mL.
3. Bdellovibrio trematodiasis plastid according to claim 1 is prevented and treated application in the agent of the red mouth pathogenic bacteria of turbot in preparation, it is characterized in that: described Bdellovibrio is Bdellovibrio (Bdellovibrio sp.Strain) BDM01, be preserved in Chinese typical culture collection center on April 28th, 2008, deposit number is CCTCC NO:M 208066.
4. prevent and treat application in the agent of the red mouth pathogenic bacteria of turbot according to the described Bdellovibrio trematodiasis of claim 1 plastid bacterium liquid in preparation, it is characterized in that: described Bdellovibrio trematodiasis plastid is to splash to prevent and treat the agent of the red mouth pathogenic bacteria of turbot in water body in the application that preparation prevents and treats in the agent of the red mouth pathogenic bacteria of turbot, makes that Bdellovibrio trematodiasis plastid final concentration is 10-10 in the water body
7Pfu/mL.
5. prevent and treat application in the agent of the red mouth pathogenic bacteria of turbot according to the described Bdellovibrio trematodiasis of claim 1 plastid bacterium liquid in preparation, it is characterized in that: described Bdellovibrio trematodiasis plastid is that feedstuff is soaked 15～45min with preventing and treating the agent of the red mouth pathogenic bacteria of turbot in the application that preparation prevents and treats in the agent of the red mouth pathogenic bacteria of turbot, and Bdellovibrio trematodiasis plastid concentration is 10-10 in the microbial inoculum
7Pfu/mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937330A (en) * | 2017-12-14 | 2018-04-20 | 华南理工大学 | The application of indoles or indoles with calcium ions and magnesium ions in promoting ocean bdellovibrio bdelloplast bacterial to be formed |
| CN107974418A (en) * | 2017-12-14 | 2018-05-01 | 华南理工大学 | One plant of ocean Bdellovibrio and the promotion plastidogenetic application of leech under ampicillin |
| CN107988136A (en) * | 2017-12-14 | 2018-05-04 | 华南理工大学 | One plant of ocean Bdellovibrio and the promotion plastidogenetic application of leech under ampicillin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275119A (en) * | 2008-01-24 | 2008-10-01 | 华南理工大学 | Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof |
| CN101642475A (en) * | 2009-08-28 | 2010-02-10 | 华南理工大学 | Microbial preparation for preventing and treating mastitis and applications thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275119A (en) * | 2008-01-24 | 2008-10-01 | 华南理工大学 | Application of bdellovibrio to eliminating pathogen in freshwater product and culture water thereof |
| CN101642475A (en) * | 2009-08-28 | 2010-02-10 | 华南理工大学 | Microbial preparation for preventing and treating mastitis and applications thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937330A (en) * | 2017-12-14 | 2018-04-20 | 华南理工大学 | The application of indoles or indoles with calcium ions and magnesium ions in promoting ocean bdellovibrio bdelloplast bacterial to be formed |
| CN107974418A (en) * | 2017-12-14 | 2018-05-01 | 华南理工大学 | One plant of ocean Bdellovibrio and the promotion plastidogenetic application of leech under ampicillin |
| CN107988136A (en) * | 2017-12-14 | 2018-05-04 | 华南理工大学 | One plant of ocean Bdellovibrio and the promotion plastidogenetic application of leech under ampicillin |
| CN107937330B (en) * | 2017-12-14 | 2020-07-28 | 华南理工大学 | Application of indole or indole and calcium magnesium ions in promoting formation of bdelloplast of marine bdellovibrio bacteriovorus |
| CN107974418B (en) * | 2017-12-14 | 2020-09-22 | 华南理工大学 | Marine bdellovibrio bacteriovorus and application thereof in promoting formation of bdelloplast under ampicillin |
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