CN102038264B - Preparation method for agaricus bisporus enzymatic hydrolysis peptide oral liquid - Google Patents
Preparation method for agaricus bisporus enzymatic hydrolysis peptide oral liquid Download PDFInfo
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- CN102038264B CN102038264B CN2010106178048A CN201010617804A CN102038264B CN 102038264 B CN102038264 B CN 102038264B CN 2010106178048 A CN2010106178048 A CN 2010106178048A CN 201010617804 A CN201010617804 A CN 201010617804A CN 102038264 B CN102038264 B CN 102038264B
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Abstract
The invention discloses a preparation method for agaricus bisporus enzymatic hydrolysis peptide oral liquid. The preparation method comprises the following steps of: selecting a fresh agaricus bisporus raw material for pulping to obtain agaricus bisporus slurry; placing the agaricus bisporus slurry in an enzymatic reactor; accurately weighing acid protease and papain and adding the acid protease and the papain into the enzymatic reactor; heating to the temperature of 50 to 60 DEG C; adjusting a pH value to be maintained between 4.5 and 5.5; performing enzymolysis for 5 to 6 hours with stirring; inactivating the protease when the proteases are digested to the predetermined hydrolysis degree of 65 percent; and centrifuging, concentrating, then preparing, clarifying, filling and sterilizing to obtain a finished product. The content of the peptide obtained by the preparation method is high; the prepared oral liquid has a golden color, a good taste and no fishy smell; the product has the advantages that: the safety is high, the nutritive value is high, the process is simple, the preparation method is easy to operate, the equipment investment is less, and the production cost is low; therefore, the preparation method is suitable for industrialized production.
Description
Technical field
The invention belongs to nutriment and health product technology field, relate to use a kind of with the oral liquid of two spore mushrooms as raw material.
Background technology
Active peptide is popular research topic of current Inpharm and food circle and the function factor that has development prospect; Both be absorbed by the body easily, and had delaying senility again, suppress tumour, regulate hormone, improve functions such as immune, antibiotic, antiviral, hypotensive and reducing blood lipid.Fields such as bio-pharmaceuticals, health products, food, biological reagent, animal feed have been applied at present, like insulin, Cerebrolysin, collagen peptide, newborn peptide, deer peptide, rice peptide, Soybean Peptide, egg white peptide, hemepeptide etc.
Two spore mushrooms are important edible mushrooms of a kind of worldwide cultivation, and China is producing country and the exported country of first in the world pair of spore mushroom, but its deep processing level is very backward, and high value-added product does not almost have.Two spore mushroom protein contents occupy first of the edible mushroom, and the dried mushroom protein of every 100g is 38～40g, contains 18 seed amino acids, and wherein 8 kinds is the necessary amino acid of human body, and lysine and leucine content are quite abundant, also contain abundant Cobastab
1, B
2, C, D, P, have health cares such as reducing blood lipid, antiviral, antitumor, enhancing body immunity.Therefore, be rich in the active peptide health products with two spore mushrooms productions and have good commercial value and application prospect, but do not appear in the newspapers as yet about two spore mushroom proteolysis peptides and products thereof both at home and abroad.
Summary of the invention
Technical problem to be solved by this invention provides the preparation method of simple, manageable pair of spore mushroom proteolysis of a kind of technology peptide oral liquid.
Solve following problem, the technical scheme that the present invention adopts is: the preparation method of a kind of pair of spore mushroom proteolysis peptide oral liquid may further comprise the steps:
Pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1-1.5, making beating obtains two spore mushroom slurries;
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.55%～0.60% and 0.20%～0.25% papain adds in the hydrolysis reactor; Be heated to 50～60 ℃; Regulate the pH value and make it maintain 4.5～5.5, stir enzymolysis 5～6h down;
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation;
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained;
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/2～1/4;
Allotment: adding accounts for concentrate quality 5～10% sucrose, 1～3% honey, 0.2～0.3% citric acid, 0.05～0.2% xanthans and sodium carboxymethylcellulose in the concentrate of above gained, the two spore mushroom proteolysis peptide oral liquids of allotment;
Clarification: with the food-grade shitosan that adds 0.1～1% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization again, can obtain finished product.
As preferred scheme, when pretreatment of raw material, with two spore mushroom raw material sections back and the mixed of water with 1:1, making beating 2-3min obtains two spore mushroom slurries.
As preferred scheme, when enzymolysis, two spore mushroom slurries are placed enzymolysis reactor; Accurately measuring the acid protease that accounts for two spore mushroom slurries quality 0.58% and 0.22% papain adds in the hydrolysis reactor; Be heated to 55 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 5.0, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
As preferred scheme, when concentrating: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/3.
The present invention is a raw material with two spore mushrooms; Adopt the two spore mushroom peptide oral liquids of complex enzyme biological enzymolysis technology preparation, this method selects suitable acid protease and papain to be hydrolyzed through pretreatment of raw material; Optimizing hydrolysising condition reaches more than 65% protein hydrolysis ratio; Go out again enzyme, centrifugation, concentrate, products therefrom adopts the analysis of Sephadex-50 gel filtration chromatography, the gained peptide molecular weight mainly is distributed between 600～3000D; The oral liquid golden yellow color that makes, good, the no fishy smell of mouthfeel, Product Safety is good, be of high nutritive value.
The enzyme digestion reaction mild condition of the method for the invention; Reaction temperature is low, and the time is short, and product is controlled easily; Whole process of production is pollution-free; Product Safety is good, and products obtained therefrom has that mouthfeel is good, peptide content is high, the nutritional labeling storage rate is high, be easy to digest and assimilate, and can be used for bio-pharmaceuticals and nutrient and healthcare products; And it is simple to have technology, processing ease, and equipment investment is few, and the advantage that production cost is low is suitable for suitability for industrialized production.
The specific embodiment
Embodiment 1
Pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1, making beating 2-3min obtains two spore mushroom slurries.
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.58% and 0.22% papain adds in the hydrolysis reactor; Be heated to 55 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 5.0, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation.
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained.
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/3.
Allotment: add in the concentrate of above gained and account for concentrate quality 8% sucrose, 2% honey, 0.2% citric acid, 0.1% xanthans and sodium carboxymethylcellulose (mixture of CMC-Na), the two spore mushroom proteolysis peptide oral liquids of allotment.
Clarification: with the food-grade shitosan that adds 0.5% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization, can obtain finished product.
Embodiment 2
Pretreatment of raw material: pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1, making beating obtains two spore mushroom slurries.
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.55% and 0.20% papain adds in the hydrolysis reactor; Be heated to 50 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 4.5, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation;
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained;
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/2;
Allotment: add in the concentrate of above gained and account for concentrate quality 5% sucrose, 1% honey, 0.2% citric acid, 0.05% xanthans and sodium carboxymethylcellulose (mixture of CMC-Na), the two spore mushroom proteolysis peptide oral liquids of allotment;
Clarification: with the food-grade shitosan that adds 0.1% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization, can obtain finished product.
Embodiment 3
Pretreatment of raw material: pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1.5, making beating obtains two spore mushroom slurries.
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.6% and 0.25% papain adds in the hydrolysis reactor; Be heated to 50 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 5.5, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation;
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained;
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/4;
Allotment: add in the concentrate of above gained and account for concentrate quality 10% sucrose, 3% honey, 0.3% citric acid, 0.2% xanthans and sodium carboxymethylcellulose (mixture of CMC-Na), the two spore mushroom proteolysis peptide oral liquids of allotment;
Clarification: with the food-grade shitosan that adds 1% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization, can obtain finished product.
Embodiment 4
Pretreatment of raw material: pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1, making beating obtains two spore mushroom slurries.
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.58% and 0.20% papain adds in the hydrolysis reactor; Be heated to 50 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 5, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation;
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained;
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/3;
Allotment: add in the concentrate of above gained and account for concentrate quality 6% sucrose, 3% honey, 0.2% citric acid, 0.1% xanthans and sodium carboxymethylcellulose (mixture of CMC-Na), the two spore mushroom proteolysis peptide oral liquids of allotment;
Clarification: with the food-grade shitosan that adds 0.4% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization, can obtain finished product.
Claims (5)
1. the preparation method of two spore mushroom proteolysis peptide oral liquids is characterized in that may further comprise the steps:
Pretreatment of raw material: select fresh two spore mushroom raw materials, with two spore mushroom raw material sections back and the mixed of water with 1:1-1.5, making beating obtains two spore mushroom slurries;
Enzymolysis: two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.55%～0.60% and 0.20%～0.25% papain adds in the hydrolysis reactor; Be heated to 50～60 ℃; Regulate the pH value and make it maintain 4.5～5.5, stir enzymolysis 5～6h down;
Enzyme goes out: when protein digestion during to predetermined degree of hydrolysis 65%, stop to stir, shift out reactor, be warmed up to 95 ℃ rapidly, heating 10min is with the protease deactivation;
Centrifugation: enzymolysis liquid is collected supernatant at the centrifugal 20min of 4000 rpm, is two spore mushroom hybrid peptides of two enzyme composite hydrolysis gained;
Concentrate: the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/2～1/4;
Allotment: adding accounts for concentrate quality 5～10% sucrose, 1～3% honey, 0.2～0.3% citric acid, 0.05～0.2% xanthans and sodium carboxymethylcellulose in the concentrate of above gained, the two spore mushroom proteolysis peptide oral liquids of allotment;
Clarification: with the food-grade shitosan that adds 0.1～1% in the two spore mushroom proteolysis peptide oral liquids of gained, high-speed stirred is even, leaves standstill again, clarifies 3～6h naturally, obtain the clear supernatant after, carry out can, sterilization, can obtain finished product.
2. the preparation method of according to claim 1 pair of spore mushroom proteolysis peptide oral liquid is characterized in that: when pretreatment of raw material, with two spore mushroom raw material sections back and the mixed of water with 1:1, making beating 2-3min obtains two spore mushroom slurries.
3. the preparation method of according to claim 1 and 2 pair of spore mushroom proteolysis peptide oral liquid is characterized in that: when enzymolysis, two spore mushroom slurries are placed enzymolysis reactor; Accurate weighing accounts for the acid protease of two spore mushroom slurries quality 0.58% and 0.22% papain adds in the hydrolysis reactor; Be heated to 55 ℃, regulate the pH value with the HCl solution of 0.1M and make it maintain 5.0, will constantly stir in the enzymolysis process; And keep stable temperature and pH value, enzymolysis 5～6h.
4. the preparation method of according to claim 1 and 2 pair of spore mushroom proteolysis peptide oral liquid is characterized in that: when concentrating, the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/3.
5. the preparation method of according to claim 3 pair of spore mushroom proteolysis peptide oral liquid is characterized in that: when concentrating, the supernatant of centrifugal collection is rotated evaporation and concentration, makes it volume and be reduced to original 1/3.
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| CN102224879B (en) * | 2011-06-09 | 2012-09-26 | 伍曾利 | Preparation method and application of champignon polypeptide |
| CN103110092B (en) * | 2013-02-06 | 2014-10-22 | 徐州工程学院 | Pleurotus eryngii active protein peptide health-care beverage |
| CN108497503A (en) * | 2017-06-15 | 2018-09-07 | 西南大学 | A kind of processing method of JINZHENGU protein anti-oxidation peptide oral solution |
| CN108968076A (en) * | 2018-07-03 | 2018-12-11 | 罗铁柱 | A kind of production method of agaricus bisporus proteolysis peptide oral liquid |
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| CN101613418A (en) * | 2009-07-22 | 2009-12-30 | 柏林 | A kind of method of extracting agaricus lentinan |
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