CN102031235B - Enterococcus faecium ANSE228 and application thereof - Google Patents
Enterococcus faecium ANSE228 and application thereof Download PDFInfo
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- CN102031235B CN102031235B CN2010105399880A CN201010539988A CN102031235B CN 102031235 B CN102031235 B CN 102031235B CN 2010105399880 A CN2010105399880 A CN 2010105399880A CN 201010539988 A CN201010539988 A CN 201010539988A CN 102031235 B CN102031235 B CN 102031235B
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Abstract
The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.
Description
Technical field
The present invention relates to a kind of enterococcus faecalis and application thereof.
Background technology
Along with society and the reach of science, food safety receives people's attention day by day, because dangerous food not only is detrimental to health and life, even also possibly have a negative impact to the descendants.Green, safe food is appealed by society, and the safety of feed is exactly the safety of food.
Microbiotic has vital role as fodder additives for aspects such as promoting animal growth, raising disease resistance.But its a large amount of for a long time uses have caused the generation of some negative effects, have for example destroyed the microecological balance of animal self, have increased the disease of alimentary tract of livestock husbandry and birds odds, and can make pathogenic bacteria sport Resistant strain etc.In animal body residual of what is more important, microbiotic passed to the mankind through food chain, brings hidden danger to health of people.Given this, a lot of countries all forbid in feed and limit the use of microbiotic as fodder additives.Therefore, the exploitation of microbiotic substitute products has also become the emphasis of countries in the world researchs.Wherein probiotics is because its special advantages, like non-specific antibacterial and the germicidal action of wide spectrum, environmental protection, noresidue etc., using the alternative field of microbiotic just obtaining increasing attention.
This research with enterococcus faecalis (Enterococcus faecium) as research object; Integrated existing theory and practice understanding of benefit being given birth to enterococcus faecalis; Filter out have anti-microbial activity, the benefit of resistance and security gives birth to enterococcus faecalis; The zymotechnique of optimizing this bacterium is also studied it as the influence of probiotics to breeding performonce fo animals, and the application and the practice of giving birth to enterococcus faecalis for benefit lay the foundation.
Summary of the invention
The object of the present invention is to provide a kind of enterococcus faecalis.
Another object of the present invention is to provide the application of above-mentioned enterococcus faecalis.
The present invention also aims to provide a kind of probiotics.
A purpose more of the present invention is to provide the application of above-mentioned probiotics.
In order to realize the object of the invention; The present invention provides a kind of enterococcus faecalis (Enterococcusfaecium) ANSE228; Its deposit number is CGMCC No.4082; Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation date: on August 16th, 2010.Said enterococcus faecalis ANSE228 obtains through technologies such as separation repeatedly, purifying, rejuvenation, advantage such as the remarkable and anti-adversity of strong, the beneficial natural disposition of biologically active is good.
The cultural method of the ANSE228 of enterococcus faecalis described in the present invention is following: (seed liquor is that the single bacterium colony of picking is cultivated in the seed liquor substratum, and its viable bacteria concentration is with 10 to get enterococcus faecalis ANSE228 seed liquor 1-5ml
9The CFU/ml meter; The culture of seed liquid base is identical with the substratum and the culture condition of fermentation with culture condition), be inoculated into and carry out the shake flask fermentation cultivation in the 50-100ml substratum.Said substratum is made up of following component: Semen Maydis powder 10-25g/L, dregs of beans 10-30g/L, glucose 0-15g/L, potassium hydrogenphosphate 0.1-0.2g, potassium primary phosphate 1.0-2.0g, manganese sulfate monohydrate 0.5-2.0g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.5-2.0g, zero(ppm) water 800-1200mL, the pH value is 6.5-7.5.
Wherein, be preferably: Semen Maydis powder 15g, dregs of beans 20g, glucose 5g, potassium hydrogenphosphate 0.15g, potassium primary phosphate 1.5g, manganese sulfate monohydrate 1.0g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 1.5g, zero(ppm) water 1000mL, the pH value is 7.0.
The condition of shake flask fermentation described in the present invention is: leavening temperature 25-45 ℃, and fermentation time 18-36h, pH value 6.5-7.5 leaves standstill cultivation; Be preferably: 37 ℃ of leavening temperatures, fermentation time 24h, pH value 7.0.
10L fermentor tank pilot scale substratum is identical with the shake flask fermentation substratum.10L fermentor tank pilot scale fermentation condition is: liquid amount is the 4-7L substratum, and inoculum size is the 30-100ml shake-flask seed liquid, and leavening temperature is 25-45 ℃, fermentation time 18-36h, pH value 6.5-7.5, mixing speed 200-400r/min.Wherein, be preferably: liquid amount is the 5L substratum, and inoculum size is that 50ml shake-flask seed liquid leavening temperature is 37 ℃, fermentation time 24h, pH value 7.0, mixing speed 300r/min.
The present invention also provides enterococcus faecalis ANSE228 application in suppressing white dysentery Salmonellas, intestinal bacteria and/or streptococcus aureus.Said bacterial strain has significant beneficial natural disposition, can significantly suppress the growth and breeding of pathogenic bacterium such as white dysentery Salmonellas, intestinal bacteria and streptococcus aureus.
The present invention also provides said enterococcus faecalis ANSE228 application in the hot environment of SGF and/or simulation cholate and/or 75-90 ℃.Said bacterial strain has stronger resistance, can tolerate simulation hydrochloric acid in gastric juice, simulation cholate and hot environment, and can keep viable bacteria survival rate higher more than 80%.
The method of inspection of the beneficial natural disposition of enterococcus faecalis ANSE228 of the present invention is following:
On the aseptic technique platform, preparation thickness is that the MRS about 4mm is dull and stereotyped, is 10 with pathogenic bacterium (intestinal bacteria, streptococcus aureus and Salmonellas) concentration
6Bacteria suspension 100-200 μ L coat on this flat board, with the sterilization stainless steel tubule (the circular tubule of internal diameter 6mm, external diameter 8mm, high 10mm, the two ends of pipe are smooth) be placed on the substratum; Gently the pressurization, make it contact tight with substratum, treat several minutes after; In each tubule, drip the enterococcus faecalis ANSE228 activated spawn bacterium liquid of some amount respectively; Do not make it excessive, cultivate 18-36h, measure antibacterial circle diameter then for 35 ℃-40 ℃.Each tests three repetitions, averages.
Wherein, said MRS flat board is with the MRS substratum behind 121 ℃ of high-temperature sterilizations, pours the flat board after the sterilization into, and it is dull and stereotyped that the cooling back forms the thick MRS of ganoid 4mm.The prescription of said MRS substratum is: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, K
2HPO
42g, diammonium hydrogen citrate 2g, glucose 20g, sodium acetate 5g, Tween-801mL, MgSO
47H
2O0.58g, MnSO
44H
2O 0.25g, agar 16g, zero(ppm) water 1000ml, pH=7.0.
The method of inspection of enterococcus faecalis resistance of the present invention is following:
1, high temperature tolerance test: get 5mL enterococcus faecalis ANSE228 viable bacteria bacterium liquid and be injected in the centrifuge tube 1,, select suitable dilution diluent, coating on MRS dull and stereotyped 1 with ten times of stepwise dilutions of SPSS.Other gets 5mL enterococcus faecalis ANSE228 viable bacteria bacterium liquid and is injected in the centrifuge tube 2; Place 75-90 ℃ of water-bath to heat 10-20min; The enterococcus faecalis ANSE228 viable bacteria bacterium liquid of getting after the heating carries out ten times of stepwise dilutions with SPSS; Select and aforementioned identical dilution diluent coating on MRS dull and stereotyped 2.Flat board 1 is cultivated 18-36h with dull and stereotyped 2 under 30-45 ℃ of condition, calculate the quantity of enterococcus faecalis heating front and back.
2, the resistance test of SGF: stomach en-is dissolved in the 0.5%-0.85% saline water, and making its final concentration is 3g/L, and adjusts the pH value to 2.0-4.0 with the NaOH of concentrated hydrochloric acid or 10%.Get in the SGF that 0.5mL enterococcus faecalis ANSE228 bacteria suspension joins 4.5mL (i.e. ten times of stepwise dilutions), and fully mixed on vibrator rapidly, place 30-45 ℃ to leave standstill and cultivate 2-4h then.In 2h, 4h, take out nutrient solution respectively and count remaining viable count immediately, compare with former viable count.
3, the resistance test of simulation cholate:
Process the solution of 1g/L-1.5g/L with pancreatin, and in solution, add 0.3% pig cholate, use 10% NaOH adjustment pH to be 7.0-9.0, use 0.45 μ m micro-filtrate membrane filtration and degerming then.0.5ml enterococcus faecalis ANSE228 viable bacteria bacterium liquid is inoculated in the 4.5ml simulation cholate, obtains nutrient solution behind the cultivation 24h, count the viable count of remaining enterococcus faecalis ANSE228.With bacterium liquid ten times of stepwise dilutions in sterile saline, and carry out the dull and stereotyped coating of MRS, place 30-45 ℃ to leave standstill cultivation 18-36h (enterococcus faecalis generally will be cultivated just can grow up to obvious single bacterium colony more than the 18h) then.
SGF and the method for counting of simulating in the cholate resistance test:
Sample selects suitable extent of dilution to be used for counting with ten times of gradient dilutions of SPSS.Adopt the MRS nutrient agar, get the 0.5mL diluent and be coated on the flat board, cultivate 24h at 37 ℃.
The present invention also provides a kind of probiotics, and it contains above-mentioned enterococcus faecalis ANSE228.Said probiotics is the enterococcus faecalis ANSE228 that strong, the beneficial natural disposition of biological activity is remarkable and anti-adversity is good that obtains through technologies such as separation repeatedly, purifying, rejuvenation; Obtain fermented liquid through production technique such as liquid submerged fermentations, after drying, make then.
The present invention also provides the application of above-mentioned probiotics in breed, its be with probiotics as additive, add in the drinking-water or feed of cultivated animals.Wherein, the addition of said enterococcus faecalis ANSE228 in drinking-water is 10
8-10
11CFU/L drinking-water; The addition of said enterococcus faecalis ANSE228 in feed is 10
7-10
10The CFU/kg feed.Cultivated animals described in the present invention comprises fryer, laying hen and pig, also comprises fish, duck or ruminating animal, like ox or sheep.
Enterococcus faecalis ANSE228 of the present invention is less at its consumption, for example in feed, is merely 10
7Can significantly improve the production performance of animal during CFU/kg, make that the fryer feedstuff-meat ratio reduces by 33.14%, the piglet death rate is reduced to 0%, the laying hen feedstuff-egg ratio reduces by 19.8%, has significantly improved the production performance of animal, has reduced production cost.
Beneficial effect of the present invention is:
1. enterococcus faecalis ANSE228 of the present invention has significant beneficial natural disposition, can significantly suppress the growth and breeding of pathogenic bacterium such as white dysentery Salmonellas, intestinal bacteria and streptococcus aureus.
2. enterococcus faecalis ANSE228 of the present invention has stronger resistance, can tolerate simulation hydrochloric acid in gastric juice, simulation cholate and hot environment, and can keep higher viable bacteria survival rate, and its viable bacteria survival rate can reach more than 80%.Because temperature is higher in the process that feed processing is granulated, and reaches 75-90 ℃, so bacterial strain need tolerate that this TR could guarantee that animal has is viable bacteria, and viable bacteria just has beneficial natural disposition.Tolerance hydrochloric acid in gastric juice and cholate are in order to make enterococcus faecalis in animal digestive tract, can bring into play its effect.
3. the present invention has excavated the new purposes of known enterococcus faecalis, has opened up a new Application Areas.
4. after the probiotics that enterococcus faecalis ANSE228 processes among the present invention got into animal intestinal, the powerful rapid activation of enterococcus faecalis ANSE228, propagation formed useful dominant microflora simultaneously in the intestinal microecology environment.
5. enterococcus faecalis ANSE228 of the present invention has significant beneficial natural disposition and resistance, not only can improve the food utilization efficiency of animal; Practice thrift cost; And improved in the animal body stable state of environment in the digestive tube, and promote absorbing of nutrition, promote growth of animal; Effect such as increase economic efficiency is indicating good prospects for application.
Description of drawings
Fig. 1 is the inhibition zone of enterococcus faecalis ANSE228 to the white dysentery Salmonellas.
Fig. 2 is that enterococcus faecalis ANSE228 is to colibacillary inhibition zone.
Fig. 3 is the inhibition zone of enterococcus faecalis ANSE228 to golden yellow Salmonellas.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1 enterococcus faecalis ANSE228 preparation of fermentation liquid
(deposit number is CGMCC No.4082, and viable bacteria concentration is 10 to get enterococcus faecalis ANSE228
9CFU/ml), be inoculated in and carry out shake flask fermentation in the 50ml substratum and cultivate, leavening temperature is 37 ℃, pH value 7.0, leaves standstill cultivation, fermentation time 24h.
Wherein, the shake flask fermentation substratum is made up of following component: Semen Maydis powder 15g, dregs of beans 20g, glucose 5g, potassium hydrogenphosphate 0.15g, potassium primary phosphate 1.5g, manganese sulfate monohydrate 1.0g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 1.5g, zero(ppm) water 1000mL, the pH value is 7.0.
Shake flask fermentation carries out the fermentor tank pilot plant test after finishing, and gets 50ml shake flask fermentation seed liquor and is inoculated in the 10L fermentor tank, and liquid amount is that 5L, leavening temperature are 37 ℃, pH value 7.0, mixing speed 300r/min, fermentation time 36h.Fermentor tank pilot scale substratum is formed same shake flask fermentation.
After the fermentation ends, be kept in 4 ℃ of refrigerators fermented liquid subsequent use.
The checking of embodiment 2 enterococcus faecalis ANSE228 benefit natural disposition
Preparation thickness is that the MRS about 4mm is dull and stereotyped, is 10 with pathogenic bacterium (intestinal bacteria, streptococcus aureus and Salmonellas) concentration
6Bacteria suspension 100 μ L coat on this flat board, with aseptic technique with the sterilization stainless steel tubule (the circular tubule of internal diameter 6mm, external diameter 8mm, high 10mm, the two ends of pipe are smooth); Be placed on the substratum, pressurization makes it contact tight with substratum gently; After treating several minutes, the fermented liquid (activated spawn bacterium liquid) that the embodiment 1 of dropping 200-300 microlitre prepares in each tubule does not respectively make it excessive; Cultivate 24h under 37 ℃ of temperature, measure antibacterial circle diameter then.Each tests three repetitions, averages.The result is shown in Fig. 1-3.
Wherein, said MRS flat board is with the MRS substratum behind 121 ℃ of high-temperature sterilizations, pours the flat board after the sterilization into, and it is dull and stereotyped that the cooling back forms the thick MRS of ganoid 4mm.The prescription of said MRS substratum is: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, K
2HPO
42g, diammonium hydrogen citrate 2g, glucose 20g, sodium acetate 5g, Tween-801mL, MgSO
47H
2O 0.58g, MnSO
44H
2O 0.25g, agar 16g, zero(ppm) water 1000ml, pH=7.0.
Enterococcus faecalis ANSE228 is 1.20cm to the antibacterial circle diameter of white dysentery Salmonellas, and the antibacterial circle diameter of intestinal bacteria and streptococcus aureus is respectively 1.16cm and 1.12cm.
The checking of embodiment 3 enterococcus faecalis ANSE228 resistance
1, the fermented liquid that the 5mL embodiment 1 that keeps prepares of going bail for is injected in the centrifuge tube 1, adopts ten times of stepwise dilutions, coating on MRS dull and stereotyped 1.The centrifuge tube 2 that the fermented liquid that 5mL embodiment 1 prepares will be housed again places 80 ℃ of water-baths to heat 15min, and the enterococcus faecalis bacterium liquid of getting after the heating carries out ten times of stepwise dilutions, coating on MRS dull and stereotyped 2.Before will heating at last with heating after flat board all under 37 ℃ of conditions, cultivate 24h, calculate the quantity before and after the enterococcus faecalis ANSE228 heating.
The result shows that the viable bacteria survival rate has reached 85%.
2, the resistance test of SGF: stomach en-is dissolved in 0.5% saline water, and making its final concentration is 3g/L, and adjusts pH value to 2.0 with the NaOH of concentrated hydrochloric acid or 10%.Get in the SGF that 0.5mL enterococcus faecalis ANSE228 bacteria suspension joins 4.5mL (i.e. ten times of dilutions), and fully mixed on vibrator rapidly, place 37 ℃ to leave standstill and cultivate 2h then.After handling 2h, take out nutrient solution and count remaining viable count immediately, compare with former viable count.The result shows that the viable bacteria survival rate is 96%.
3, the resistance test of simulation cholate: process the solution of 1g/L with pancreatin, and in solution, add 0.3% pig cholate, using 10% NaOH adjustment pH is 8.0, uses 0.45 μ m micro-filtrate membrane filtration and degerming then.0.5ml enterococcus faecalis ANSE228 viable bacteria bacterium liquid is inoculated in the 4.5ml simulation cholate, obtains nutrient solution behind the cultivation 24h, count the viable count of remaining enterococcus faecalis ANSE228.With bacterium liquid ten times of stepwise dilutions in sterile saline, and carry out the dull and stereotyped coating of MRS, place 37 ℃ to leave standstill cultivation 24h then.The result shows that the viable bacteria survival rate is 96%.
Embodiment 4 growth of meat chicken PTs
Select 120 the 30 healthy fryer of age in days for use, weigh, be divided into 4 treatment group at random, 5 repetitions are established in each processing, and each repeats 6 chickens, and public mother respectively accounts for 1/2.Test drinking-water is respectively: (1) common drinking-water (control group); (2) enterococcus faecalis ANSE228 viable bacteria concentration is 10 in the drinking-water
10CFU/L; (3) enterococcus faecalis ANSE228 viable bacteria concentration is 10 in the drinking-water
9CFU/L; (4) enterococcus faecalis ANSE228 viable bacteria concentration is 10 in the drinking-water
8CFU/L.Test chicken free choice feeding and drinking-water, feeding and management and immune programme for children are with reference to the feeding of broiler administrative manual.
Table 1 enterococcus faecalis ANSE228 is to the growth of meat chicken Effect on Performance
Annotate: do not contain same letter person with line data shoulder mark and represent significant difference (P＜0.05) (down together)
This test-results shows that enterococcus faecalis ANSE228 has significantly improved fryer average daily gain (P=0.0123) and feed efficiency (P=0.0006).Simultaneously, enterococcus faecalis ANSE228 viable bacteria concentration is 10 in the drinking-water
10During CFU/L, the broiler fodder transformation efficiency is the highest.
The test of embodiment 5 laying hen production performances:
Test designs for single-factor, and 576 brown commodity of Luo Man are divided into 3 processing immediately for laying hen, and each handles 6 repetitions, and each repeats 8 cages, 4 chickens of every cage.Handle 1 and be negative contrast, the basal diet of feeding does not contain any medicated feed additive; Handling 2 is over against photograph, on the basal diet basis, adds the brown kangdisu sulfas 4mg/kg of Zinc-bacitracin 20mg/kg; Handle 3 and on the basal diet basis, add 3 * 10
9The enterococcus faecalis ANSE228 probiotics of CFU/kg.Test chicken free choice feeding and drinking-water, feeding and management and immune programme for children are with reference to the layer breeding administrative manual.
Table 2 enterococcus faecalis ANSE228 is to the influence of laying hen production performance
The result of this test shows that enterococcus faecalis ANSE228 can significantly improve feedstuff-egg ratio of laying hen and the egg productivity (p＜0.05) that improves laying hen; And can reduce production costs, improve the production performance of laying hen, increase economic efficiency; Effectively substitute Antibiotic Additive, be fit to large-scale promotion and use.
The test of embodiment 6 piglet growth performances
This experiment selected 35 ± 1 ages in days, 90 of ternary (Du * length * big) the hybridization weanling pigs that mean body weight 7.86 ± 0.06kg is healthy distribute to advance three processing immediately; Each handles 5 repetitions; Each repeats 6 piglets (public female half and half), initial body weight no significant difference (p＞0.05) between each repeating groups.
Three processing are respectively: blank group, microbiotic control group (75mg/kg duomycin) and test group (add 4 * 10
9CFU/kg enterococcus faecalis ANSE228 probiotics).Test pig free choice feeding and drinking-water, feeding and management and immune programme for children are with reference to the feeding piglet administrative manual.
Table 3 enterococcus faecalis ANSE228 is to the influence of piglet growth performance
This test-results shows that enterococcus faecalis ANSE228 can significantly improve the average daily gain (p＜0.05) of piglet, improves feedstuff-meat ratio, and has significantly reduced diarrhea rate and the death rate (p＜0.05) of piglet.Improved production performance and the economic benefit of piglet, can effectively substitute Antibiotic Additive, be fit to large-scale promotion and use.
Though, used general explanation, specific embodiments and experiment in the preceding text, the present invention has been done detailed description, on basis of the present invention, can to some modifications of do or improvement, this will be apparent to those skilled in the art.Therefore, these modifications or the improvement on the basis of not departing from spirit of the present invention, made all belong to the scope that requirement of the present invention is protected.
Claims (7)
1. an enterococcus faecalis (Enterococcus faecium) ANSE228, its deposit number is CGMCC No.4082.
2. a method of cultivating the said enterococcus faecalis ANSE228 of claim 1 is characterized in that, gets enterococcus faecalis ANSE228 seed liquor 1-5ml, is inoculated into to carry out the shake flask fermentation cultivation in the 50-100ml substratum.
3. cultural method according to claim 2; It is characterized in that; Said substratum is made up of following component: Semen Maydis powder 10-25g/L, dregs of beans 10-30g/L, glucose 0-15g/L, potassium hydrogenphosphate 0.1-0.2g, potassium primary phosphate 1.0-2.0g, manganese sulfate monohydrate 0.5-2.0g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.5-2.0g, zero(ppm) water 800-1200mL, the pH value is 6.5-7.5.
4. cultural method according to claim 2 is characterized in that, the condition of said shake flask fermentation is: leavening temperature 25-45 ℃, fermentation time 18-36h, pH value 6.5-7.5, leave standstill cultivation.
5. a probiotics is characterized in that, it contains the described enterococcus faecalis ANSE228 of claim 1.
6. the application of the described probiotics of claim 5 in breed is characterized in that, probiotics as additive, is added in the drinking-water or feed of cultivated animals, and said cultivated animals is fryer, laying hen, pig, duck, ox or sheep.
7. application according to claim 6 is characterized in that, the addition of said enterococcus faecalis ANSE228 in drinking-water is 10
8-10
11CFU/L drinking-water; The addition of said enterococcus faecalis ANSE228 in feed is 10
7-10
10The CFU/kg feed.
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| CN101701201B (en) * | 2009-11-27 | 2012-06-06 | 南京农业大学 | Enterococcus faecalis and application thereof |
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| US10322151B2 (en) | 2015-06-15 | 2019-06-18 | 4D Pharma Research Limited | Compositions comprising bacterial strains |
| CN106167785A (en) * | 2016-08-29 | 2016-11-30 | 内蒙古工业大学 | A kind of E. Faecium strains and application thereof |
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| US11779613B2 (en) | 2017-06-14 | 2023-10-10 | Cj Bioscience, Inc. | Compositions comprising a bacterial strain of the genus Megasphera and uses thereof |
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