CN102020620B - Method for separating and purifying effective constituents from salvia miltiorrhiza extracting solution by applying membrane technology - Google Patents
Method for separating and purifying effective constituents from salvia miltiorrhiza extracting solution by applying membrane technology Download PDFInfo
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Abstract
The invention relates to a method for separating and purifying effective constituents from a salvia miltiorrhiza extracting solution by applying a membrane technology. The method is characterized by comprising the following steps of: (1) preparing the salvia miltiorrhiza extracting solution; (2) carrying out solid-liquid separation; (3) carrying out microfiltration to remove sediments; (4) carrying out ultrafiltration separation; (5) carrying out nanofiltration concentration; and (6) carrying out vacuum drying. The invention takes a membrane with selective permeability as a separating medium, and the effective constituents contained in the salvia miltiorrhiza extracting solution are passed or intercepted according the size of the aperture of a filter membrane, therefore the purposes of separation and concentration are achieved; and in addition, the method better realizes the separation of the effective parts of traditional Chinese medicines, sufficiently retains the medical effect of the traditional Chinese medicines and can also outstandingly shorten the production period, reduce the production cost and the energy consumption and ensure the stability of product quality when extracted separates are used for preparing traditional Chinese medicine preparations and medicinal liquors.
Description
(1) technical field: the present invention relates to a kind of from red rooted salvia the method for extracting effective components, particularly use the method for membrane technique separation and purification effective constituent from Radix Salviae Miltiorrhizae extract.
(2) prior art: the red sage root is the dry root and rhizome of dicotyledons Labiatae Labiatae red sage root Salvia miltiorrhiza Bge., and the beginning is stated from Shennong's Herbal, classifies as top gradely, is China's a kind of Chinese medicine commonly used.The red sage root has significant pharmacological action as the medicine of the cardiovascular aspect of treatment.Effective component in red sage can be strengthened myocardial contraction, improve heart function, does not increase MCO; To the blood vessel aspect, can coronary artery dilating, increase MBF; The expansion peripheral blood vessel, blood flow increases, and cerebral blood flow (CBF) descends, so be usually used in preparing Chinese traditional compound medicine or medicinal liquor.
Recent years,,, the red sage root has been carried out systematic research in conjunction with multiple subject technologies such as chemistry, molecular biology and cytobiologies along with the development of modern medicine technology.The water soluble component of the red sage root mainly contains salvianolic acid A, B, C, D, E, Salvianic acidA etc., and wherein salvianolic acid class activity is the strongest.Confirm that through pharmacological evaluation salvianolic acid B has anti peroxidation of lipid, eliminate radical and anti thrombotic action.Therefore the red sage root is widely used in bio-pharmaceuticals and protective foods aspect, has wide utilization prospect.
The separation of Radix Salviae Miltiorrhizae extract at present is many to be ultimate principle and separating impurity (organic solvent deposit, extraction etc.) with the polarity difference between composition; And concentrate also basically with single-action, economic benefits and social benefits simmer down to master, these traditional technologys in actual production in various degree exist more deficiency.At first be that loss of effective components is serious; The original content of part effective constituent in the Chinese medicine is just very low; Again through impurity removal process such as solvent deposition, extraction or diatomite filtrations; These compositions possibly all be removed, and because factors such as absorption during contamination precipitation and embedding have also caused the bigger loss of other effective constituent; Be contaminate environment in addition, in the diatomite filtration process, produce a large amount of discarded zeyssatite, caused bigger pollution, and introduce some new impurity (silicate etc.) easily, affect greatly to product stability to environment.Secondly impurity-eliminating effect is undesirable; Materials such as the tannin in the Radix Salviae Miltiorrhizae extract, starch, resin and albumen; Be difficult to eliminate with traditional method, thereby the solid yield is very high, when Radix Salviae Miltiorrhizae extract and other Chinese medicine work in-process or the allotment of basic wine, also can produces considerable influence the stability of product.Some traditional separation method (macroporous resin adsorption separation etc.) is overemphasized single component besides, makes Chinese medicine lose original compound characteristic, influence drug effect, and application security is relatively poor in food service industry.And in concentration process, mainly exist the thickening temperature height, and concentration time is long, and loss of effective components is serious, and a step concentrates the specification of quality that is difficult to realize high specific density, and equipment is prone to problems such as fouling.
(3) summary of the invention: the object of the invention just provides the application membrane technique and extracts from the red sage root
The method of separation and purification effective constituent in the liquid; To select the film of perviousness be separating medium to this method to have, according to the size in filter membrane aperture through or be trapped, reach separation, spissated purpose, realized the separation of effective ingredient in Chinese, fully kept the drug effect of Chinese medicine.While is according to the physico-chemical property of Radix Salviae Miltiorrhizae extract; With corresponding investigation index, material, film pipe model and the operational condition of film are optimized, realized the matching of film device and red rooted salvia; Improved the resistant to pollution ability of film device, the separation efficiency of film device is further improved; Simultaneously can also significantly shorten the production cycle, reduce production costs and energy consumption, quality product is stable when guaranteeing to prepare Chinese medicine preparation and medicinal liquor.
The inventive method comprises the steps:
(1) preparation of Radix Salviae Miltiorrhizae extract
Red rooted salvia is extracted with three jars of adverse current methods, get extracting solution;
(2) solid-liquid separation
Radix Salviae Miltiorrhizae extract is carried out spinning, and the time is 20-30min, and rotating speed is more than the 6000r/min, gets centrifugate;
(3) micro-filtration removes and sinks
It is that 0.05～0.2um, membrane material are that the ceramic membrane unit of AL2O3 carries out the micro-filtration separating treatment that above-mentioned centrifugate is adopted membrane pore size; 30 ℃～60 ℃ of service temperatures, working pressure 0.2Mpa～0.5Mpa; After micro-filtration separate to finish, with the pure water of 3 times of amounts of trapped fluid given a baby a bath on the third day after its birth time in the trapped fluid top, temperature is washed on the top: 30～60 ℃, top are washed pressure: 0.2Mpa～0.5Mpa, micro-filtrate; Require the micro-filtrate clear, turbidity≤3.00NTU, effective constituent salvianolic acid B transmitance>=95%;
(4) ultra-filtration and separation
Above-mentioned micro-filtrate is carried out ultrafiltration, working pressure with the polyvinylidene fluoride (PVDF) ultrafiltration membrane pipe of 1000～10000 molecular weight: 0.8～1.0Mpa; Service temperature: 40～45 ℃, after ultra-filtration membrane separated end, it was inferior with the pure water of 2 times of amounts of trapped fluid to be given a baby a bath on the third day after its birth in the trapped fluid top, and temperature is washed on the top: 40～45 ℃, pressure: 0.8～1.0Mpa is washed on the top, gets ultrafiltrated;
Require the ultrafiltrated clear, salvianolic acid B transmitance>=95%, turbidity≤1.50, salvianolic acid B component content>=60% in the solid substance;
(5) nanofiltration concentrates
It is 200 molecular weight that above-mentioned ultrafiltrated is adopted the film model, and membrane material is that the nf membrane unit of polymeric amide carries out concentration, 40～45 ℃ of thickening temperatures, working pressure: 0.15～0.18Mpa, cycles of concentration: 10～15 times, get trapped fluid; Red sage root nanofiltration trapped fluid specification of quality: salvianolic acid B rejection >=95%, salvianolic acid B component content >=80% in the solid substance;
(6) vacuum-drying
Red sage root nanofiltration trapped fluid is carried out vacuum-drying under 50～80 ℃, promptly get.
The process for extracting of said red rooted salvia is red rooted salvia on average to be added among three jars of the A, B, C to carry out countercurrent extraction earlier; Extract temperature: 60～70 ℃; Extract solvent and be 30% ethanol of 8～12 times of amounts of medicinal material weight; Form the stage in gradient, A jar extraction time is respectively 5min, 15min, and B jar extraction time is 10min; In the extraction stage, each jar extraction time is 2h; Extracting ending phase, extraction time is 2h; After the end merging of each jar extracting solution is promptly got Radix Salviae Miltiorrhizae extract.
It is the key factor of membrane separation purification effective component in red sage that the material of above-mentioned film and model are selected.Select for use suitable membrane can play good separation and purification effect, reduce film simultaneously and pollute.
The micro-filtration ceramic-film tube that we select 0.05～0.2um aperture for use except that heavy unit can be removed most granule foreign composition except that heavy, and the step Pollution of Ultrafiltration Membrane is protected the ultra-filtration membrane core after reducing.Ceramic membrane has acid and alkali-resistance, resistant to elevated temperatures characteristics simultaneously, and membrane flux is big, convenience for washing.
Organic film material at present commonly used has FM, polyacrylonitrile, polyetherketone, polysulfones, polymeric amide etc., by its avidity classification to water, is broadly divided into two types: hydrophobic film material and hydrophilic film material.Hydrophilic film such as FM, polyacrylonitrile material is few to solute absorption, and molecular weight cut-off is less, but poor heat stability, physical strength, chemical proof property, antibiotic erosional competency are not high usually; Hydrophobic film such as polysulfones, pvdf mechanical strength is high, high temperature resistant, anti-solvent, anti-biological degradation, but the permeable speed of film is low, and contamination resistance is lower.Its anti-pH scope of the organic membrane of unlike material is also roughly different, and PAN, PS, PVDF are pH2～13 in general, and CA is pH 5～6.Radix Salviae Miltiorrhizae extract is to get with 30% extraction using alcohol, the relative molecular weight of its effective constituent generally between 500～2000, pH value 4～8, according to above physico-chemical property, therefore selecting material for use is the nf membrane of the ultra-filtration membrane and the polymeric amide of pvdf.
The dry thing technical indicator of gained of the present invention:
Product purity: the effective component in red sage rate of transform >=85%, active constituent content >=90%.
Product color: inclusion-free color and luster.
Product fragrance: have the distinctive smell of these article, do not have tangible peculiar smell.
Shape of product: loose powder shape
Product moisture :≤5.0% according to GB/T 14769 execution.
The product ash content :≤5.0% according to GB/T 14770 execution.
Fineness degree (80 order percent of pass): >=95%
Red sage root index components detection method:
" red rooted salvia index components detection method in the Chinese pharmacopoeia, through checking, this method was stable, circulation ratio is better according to version in 2005.Concrete grammar is following:
The content of danshinolic acid B measuring method:
1, adopts high effective liquid chromatography for measuring (with reference to Chinese Pharmacopoeia 2005 editions)
2, the test of chromatographic condition and system suitability is a weighting agent with the octadecylsilane chemically bonded silica; With methyl alcohol-acetonitrile-formic acid-water (30: 10: 1: 59) be moving phase; The detection wavelength is 286nm.Number of theoretical plate calculates by the salvianolic acid B peak should be not less than 2000.
3, it is an amount of that the preparation precision of reference substance solution takes by weighing the salvianolic acid B reference substance, adds 75% methyl alcohol and process the solution that every 1ml contains 0.14mg, promptly gets.
4, the about 0.2g of these article powder (crossing sieve No. three) is got in the preparation of need testing solution, and accurate the title decides, and puts in the tool plug Erlenmeyer flask, the accurate 75% methyl alcohol 50ml that adds; Claim to decide weight, reflux 1 hour is taken out, and puts cold; Claim again to decide weight, supply the weight that subtracts mistake, shake up with 75% methyl alcohol; Filter, get subsequent filtrate, promptly get.
5, accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of assay method inject liquid chromatograph, measure, and promptly get.
Chemical ingredients is very complicated in the Radix Salviae Miltiorrhizae extract; Except that containing anthraquinone class, flavonoid, carbohydrate, amino acid, odor component (being mainly organic acid and ester thereof), steroidal, secoiridoid and glycosides compound thereof, also contain amounts of protein, colloid, tannin, starch, Mierocrystalline cellulose, inorganic salt and other material and many particulates, floss.Chemical ingredients relative molecular mass wider distribution; Be tens to millions of dalton; Wherein the material of high molecular weight mainly is non-medical composition or the relatively poor compositions of medical such as colloid and Mierocrystalline cellulose; And the relative molecular mass of effective constituent such as anthraquinone class, carbohydrate, steroidal, secoiridoid, glycoside etc. is less, generally all below 50000.Like the main effective constituent salvianolic acid B (C36H30O16) in the red sage root, its molecular weight has only 718.61.This shows; The relative molecular mass difference of effective constituent and non-pharmaceutical compound is very big in the Radix Salviae Miltiorrhizae extract; So select for use the ultra-filtration membrane of suitable molecular weight cutoff that extracting solution is separated; Can well realize removing impurity and spissated purpose,, guarantee the stability of product so that quantitatively allocate with other Chinese medicine work in-process or basic wine.
This technology and traditional technology are essentially different, and traditional separation purifying technique mainly is to utilize the difference of different components solubleness in different solvents and realize separating; Concentrated then be the difference of utilizing volatility between solute and the solvent, make the solvent vaporization with the method that adds heat energy, solute then is nonvolatile, thereby obtains a favourable vapor liquid equilibrium condition, reaches spissated purpose.
And this technology is that to select the film of perviousness be separating medium to have; Under the effect of certain motivating force (pressure difference); In the material composition of different molecular weight according to the filter membrane aperture the size through or be trapped; Reach separation, spissated purpose, it has realized the separation of effective ingredient in Chinese, has fully kept the drug effect of Chinese medicine.Simultaneously in this technology; We are according to the physico-chemical property of Radix Salviae Miltiorrhizae extract; Transmitance with turbidity, membrane flux variation tendency and the index components (is red sage root index components with the salvianolic acid B) that see through liquid serve as the investigation index, and material, film pipe model and the operational condition of film are optimized, and has realized the matching of film device and red rooted salvia; Improved the resistant to pollution ability of film device, the separation efficiency of film device is further improved.Using each salvianolic acid product of this technology gained shows through detection: the highest yield of Salvianic acidA, Lithospermic acid A, rosmarinic acid, salvianolic acid A and salvianolic acid B is respectively 85.55%, 86.65%, 99.27%, 92.78% and 99.34%, far above traditional technology.Content of effective is up to more than 90% in the extract.
Separate in the concentration process at Radix Salviae Miltiorrhizae extract, compare with traditional extraction separation concentration technology, the major advantage of existing technology has:
1, the present invention adopts the multiple tank countercurrent extraction to replace traditional dipping and thermal backflow, has shortened extraction time effectively, has reduced solvent load, has improved the extraction efficiency and the effective constituent rate of transform.
2, there is not phase transformation in the membrane separation concentrated compression process; Help preserving the physicochemical property of effective component in red sage; And the application of ultrafiltration, nanofiltration can as often as possible keep the plurality of active ingredients of red rooted salvia, particularly can keep some trace ingredientss in the Radix Salviae Miltiorrhizae extract.
3, improved the quality of compound preparation or medicinal liquor, than the existence of non-effective component of high molecular weight or low effective component, the concentration of effective ingredient in Chinese has been reduced, drug effect weakens, and brings big difficulty when participating in preparation compound Chinese medicinal preparation or medicinal liquor simultaneously.And the concentrated technology of membrane sepn can be removed non-effective component of relative molecular mass or low effective component to greatest extent, thereby this technology can make quality product obtain bigger raising.
4, owing to do not consume organic solvent; And do not add other any material in the separation concentration process, therefore compare with traditional method, membrane sepn concentrates technology can reduce operation; Shorten the production cycle, reduce production costs; Reduce environmental pollution, and whole technology can carry out continuously, be beneficial to scale operation.
5, the present invention has higher selectivity to the separate targets composition, can change the specification and the operational condition of microfiltration membrane according to market to requirement of products, thereby the target substance of controlling products obtained therefrom is easily formed.
(4) embodiment:
Embodiment 1
1, the preparation of Radix Salviae Miltiorrhizae extract
Will the red rooted salvia behind clean it up on average add among three jars of the A, B, C and carry out countercurrent extraction; Extract temperature: 60 ℃, extract solvent and be 30% ethanol of 8 times of amounts of medicinal material weight, form the stage in gradient; A jar extraction time is respectively 5min, 15min, and B jar extraction time is 10min; In the extraction stage, each jar extraction time is 2h; Extracting ending phase, extraction time is 2h; After the end merging of each jar extracting solution is promptly got Radix Salviae Miltiorrhizae extract;
2, solid-liquid separation
Owing to contain more solid particle polluter in the Radix Salviae Miltiorrhizae extract, directly remove and heavyly can make film forming severe contamination with ceramic membrane, reduce the work-ing life of film, thereby the pre-treatment of soup is the key that guarantees the film use properties, and can prolongs the work-ing life of film.
Adopt the Alfa Laval disc centrifuge to carry out high speed centrifugation Radix Salviae Miltiorrhizae extract, the time is 20-30min, and rotating speed is more than the 6000r/min, gets centrifugate; Deposition after centrifugal is directly drained, and requires the centrifugate clear;
3, micro-filtration removes and sinks
The micro-filtration separating treatment is mainly removed some bacteriums, suspended particle and the colloidal type material in the Radix Salviae Miltiorrhizae extract; It is that 0.05um, membrane material are that the ceramic membrane unit of AL2O3 carries out the micro-filtration separating treatment that above-mentioned centrifugate is adopted membrane pore size; 30 ℃ of service temperatures, working pressure 0.2Mpa; After micro-filtration separated end, it was inferior with the pure water of 3 times of amounts of trapped fluid to be given a baby a bath on the third day after its birth in the trapped fluid top, and temperature is washed on the top: 30 ℃, pressure: 0.2Mpa is washed on the top, gets micro-filtrate; Require the micro-filtrate clear, turbidity≤3.00NTU, effective constituent salvianolic acid B transmitance>=95%;
4, ultra-filtration and separation
Above-mentioned micro-filtrate is carried out ultrafiltration with the polyvinylidene fluoride (PVDF) ultrafiltration membrane pipe of 1000 molecular weight; Working pressure: 0.8Mpa, service temperature: 40 ℃, after ultra-filtration membrane separates end; Pure water with 2 times of amounts of trapped fluid is given a baby a bath on the third day after its birth inferior to the trapped fluid top; Temperature is washed on the top: 40 ℃, pressure: 0.8Mpa is washed on the top, gets ultrafiltrated;
Require the ultrafiltrated clear, salvianolic acid B transmitance>=95%, turbidity≤1.50, salvianolic acid B component content>=60% in the solid substance;
5, nanofiltration concentrates
It is 200 molecular weight that above-mentioned ultrafiltrated is adopted the film model, and membrane material is that the nf membrane unit of polymeric amide carries out concentration, 40 ℃ of thickening temperatures, working pressure: 0.15Mpa, cycles of concentration: 10 times, get trapped fluid; Red sage root nanofiltration trapped fluid specification of quality: salvianolic acid B rejection >=95%, salvianolic acid B component content >=80% in the solid substance;
6, vacuum-drying
Red sage root nanofiltration trapped fluid is carried out vacuum-drying under 50 ℃, promptly get.
Embodiment 2
1, the extraction temperature in the preparation of Radix Salviae Miltiorrhizae extract is 70 ℃, extracts solvent and be 30% ethanol of 12 times of amounts of medicinal material weight; Surplus with embodiment 1
2, solid-liquid separation
With embodiment 1
3, micro-filtration removes and sinks
It is that 0.2um, membrane material are that the ceramic membrane unit of AL2O3 carries out the micro-filtration separating treatment that above-mentioned centrifugate is adopted membrane pore size; 60 ℃ of service temperatures, working pressure 0.5Mpa; After micro-filtration separate to finish, with the pure water of 3 times of amounts of trapped fluid given a baby a bath on the third day after its birth time in the trapped fluid top, temperature is washed on the top: 60 ℃, top are washed pressure: 0.5Mpa, micro-filtrate, surplus with embodiment 1;
4, ultra-filtration and separation
Above-mentioned micro-filtrate is carried out ultrafiltration, working pressure with the polyvinylidene fluoride (PVDF) ultrafiltration membrane pipe of 10000 molecular weight: 1.0Mpa, service temperature: 45 ℃, temperature is washed on the top: 45 ℃, pressure: 1.0Mpa is washed on the top, ultrafiltrated; Surplus with embodiment 1;
5, nanofiltration concentrates
45 ℃ of thickening temperatures, working pressure: 0.18Mpa, cycles of concentration: 15 times, get trapped fluid; Surplus with embodiment 1;
6, vacuum-drying
Red sage root nanofiltration trapped fluid is carried out vacuum-drying under 80 ℃, promptly get.
Embodiment 3
1, the extraction temperature in the preparation of Radix Salviae Miltiorrhizae extract is 65 ℃, extracts solvent and be 30% ethanol of 10 times of amounts of medicinal material weight; Surplus with embodiment 1
2, solid-liquid separation
With embodiment 1
3, micro-filtration removes and sinks
It is that 0.09um, membrane material are that the ceramic membrane unit of AL2O3 carries out the micro-filtration separating treatment that above-mentioned centrifugate is adopted membrane pore size; 60 ℃ of service temperatures, working pressure 0.5Mpa; After micro-filtration separate to finish, with the pure water of 3 times of amounts of trapped fluid given a baby a bath on the third day after its birth time in the trapped fluid top, temperature is washed on the top: 45 ℃, top are washed pressure: 0.3Mpa, micro-filtrate, surplus with embodiment 1;
4, ultra-filtration and separation
Above-mentioned micro-filtrate is carried out ultrafiltration, working pressure with the polyvinylidene fluoride (PVDF) ultrafiltration membrane pipe of 5000 molecular weight: 0.9Mpa, service temperature: 42 ℃, temperature is washed on the top: 42 ℃, pressure: 0.9Mpa is washed on the top, ultrafiltrated; Surplus with embodiment 1;
5, nanofiltration concentrates
43 ℃ of thickening temperatures, working pressure: 0.17Mpa, cycles of concentration: 13 times, get trapped fluid; Surplus with embodiment 1;
6, vacuum-drying
Red sage root nanofiltration trapped fluid is carried out vacuum-drying under 70 ℃, promptly get.
Employed 30% ethanol (v/v) is food grade among the present invention.
Claims (2)
1. use the method for membrane technique separation and purification effective constituent from Radix Salviae Miltiorrhizae extract, it is characterized in that comprising the steps:
(1) preparation of Radix Salviae Miltiorrhizae extract
Red rooted salvia is extracted with three jars of adverse current methods, get extracting solution;
(2) solid-liquid separation
Radix Salviae Miltiorrhizae extract is carried out spinning, and the time is 20-30min, and rotating speed is more than the 6000r/min, gets centrifugate;
(3) micro-filtration removes and sinks
It is that 0.05～0.2 μ m, membrane material are Al that above-mentioned centrifugate is adopted membrane pore size
2O
3The ceramic membrane unit carry out the micro-filtration separating treatment; 30 ℃～60 ℃ of service temperatures, working pressure 0.2MPa～0.5MPa; After micro-filtration separate to finish, with the pure water of 3 times of amounts of trapped fluid given a baby a bath on the third day after its birth time in the trapped fluid top, temperature is washed on the top: 30～60 ℃, top are washed pressure: 0.2MPa～0.5MPa, micro-filtrate; Require the micro-filtrate clear, turbidity≤3.00NTU, effective constituent salvianolic acid B transmitance>=95%;
(4) ultra-filtration and separation
Above-mentioned micro-filtrate is carried out ultrafiltration, working pressure with the polyvinylidene fluoride (PVDF) ultrafiltration membrane pipe of 1000～10000 molecular weight: 0.8～1.0MPa; Service temperature: 40～45 ℃, after ultra-filtration membrane separated end, it was inferior with the pure water of 2 times of amounts of trapped fluid to be given a baby a bath on the third day after its birth in the trapped fluid top, and temperature is washed on the top: 40～45 ℃, pressure: 0.8～1.0MPa is washed on the top, gets ultrafiltrated;
Require the ultrafiltrated clear, salvianolic acid B transmitance>=95%, turbidity≤1.50, salvianolic acid B component content>=60% in the solid substance;
(5) nanofiltration concentrates
It is 200 molecular weight that above-mentioned ultrafiltrated is adopted the film model, and membrane material is that the nf membrane unit of polymeric amide carries out concentration, 40～45 ℃ of thickening temperatures, working pressure: 0.15～0.18MPa, cycles of concentration: 10～15 times, get trapped fluid; Red sage root nanofiltration trapped fluid specification of quality: salvianolic acid B rejection >=95%, salvianolic acid B component content >=80% in the solid substance;
(6) vacuum-drying
Red sage root nanofiltration trapped fluid is carried out vacuum-drying under 50～80 ℃, promptly get.
2. the method for application membrane technique according to claim 1 separation and purification effective constituent from Radix Salviae Miltiorrhizae extract; It is characterized in that: the process for extracting of said red rooted salvia is red rooted salvia on average to be added among three jars of the A, B, C to carry out countercurrent extraction earlier; Extract temperature: 60～70 ℃, extract solvent and be 30% ethanol of 8～12 times of amounts of medicinal material weight, form the stage in gradient; A jar extraction time is respectively 5min, 15min, and B jar extraction time is 10min; In the extraction stage, each jar extraction time is 2h; Extracting ending phase, extraction time is 2h; After the end merging of each jar extracting solution is promptly got Radix Salviae Miltiorrhizae extract.
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| CN109568352A (en) * | 2018-12-18 | 2019-04-05 | 合肥信达膜科技有限公司 | The film extraction process of effective component in a kind of traditional Chinese medicine liquid |
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