CN102020492A - Efficient wheat straw degradation composite bacteria organic fertilizer and preparation method thereof - Google Patents
Efficient wheat straw degradation composite bacteria organic fertilizer and preparation method thereof Download PDFInfo
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- CN102020492A CN102020492A CN2010105310307A CN201010531030A CN102020492A CN 102020492 A CN102020492 A CN 102020492A CN 2010105310307 A CN2010105310307 A CN 2010105310307A CN 201010531030 A CN201010531030 A CN 201010531030A CN 102020492 A CN102020492 A CN 102020492A
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Abstract
The invention relates to an efficient wheat straw degradation composite bacteria organic fertilizer. The efficient wheat straw degradation composite bacteria organic fertilizer is formed by fermenting wheat straw minces with efficient wheat straw degradation composite bacteria, wherein, the efficient wheat straw degradation composite bacteria are composed of a carrier and a compound bacterial agent, the carrier is corn cob powder, and the compound bacterial agent is composed of a bacterial agent I and a bacterial agent II; and the compound bacterial agent is fully mixed with wheat straw substances in an aerobic fermentation device, and wheat straws are degraded through synergistic effect of different strains, thus the degradation efficiency is improved and the degradation time is shortened, and the substances can be rapidly decomposed and recycled and then are converted into the fertilizer capable of improving soil. By utilizing the efficient wheat straw degradation bacterial agent, the problems such as land occupation caused by landfill or fire hazard, traffic hazard, environmental pollution and the like caused by incineration can be effectively solved.
Description
Technical field
The present invention relates to environmental technology field, especially a kind of wheat straw stalk efficient degradation composite bacteria fertilizer and preparation method thereof
Background technology
The described wheat straw stalk of present method contains Mierocrystalline cellulose, hemicellulose, xylogen, organic matter and nitrogen, phosphorus, potassium and trace element etc., nutritious, directly burning, not only pollutant atmosphere, form disaster hidden-trouble, cause traffic hazard and influence area, city image, and calorific value is low, is not easy to utilize; If carry out landfill disposal, though can rely on the ability of soil self that it is degraded, degradation time is long, consumes the nutrient in the soil, and utilization ratio is low; If it is banked up for a long time, land occupation not only, and also as time goes by, the thing self of banking up fermentation produces stench and percolate, and the havoc surrounding enviroment produce greatly influence to people's orthobiosis.
On the other hand, China's cultivated land resource is in short supply, and farmland quality is low; In order to solve the input that characteristics that the fundamental realities of the country that grain security problem China has a large population and a few land causes soil nutrient to be exhausted have determined constantly to increase fertilizer, but the long-term application chemical fertilizer can cause that earth hardens, disease and pest and polluted-water etc.Because advantages such as fertilizer has improving the soil, strengthen crop opposing disease and pest ability, pollution-free make the demand of fertilizer significantly promote.
To sum up, press for and seek wheat straw stalk resource technology, alleviating of harm and the influence of wheat straw stalk, and realize the abundant recovery and the cycling and reutilization of wheat straw-like materials environment, reluctant wheat straw stalk is turned waste into wealth, realize energy-saving and emission-reduction and low-carbon economy developing goal.Therefore, from the angle that environment protection and waste utilize again, press for a kind of wheat straw stalk and of can rapidly and efficiently degrading with its method of utilizing again.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of wheat straw stalk efficient degradation composite bacteria fertilizer and preparation method thereof is provided.This method utilizes the wheat straw stalk to produce fertilizer by the microorganism Synergistic degradation, realizes the waste cycling and reutilization, economizes the land resource, and eliminates disaster hidden-trouble and hidden peril of accident, improves the level of resources utilization, improves the soil.
The present invention is achieved through the following technical solutions:
A kind of wheat straw stalk efficient degradation composite bacteria fertilizer, this wheat straw stalk efficient degradation composite bacteria fertilizer is to be formed by the broken end of wheat straw stalk efficient degradation composite bacteria fermentation wheat straw stalk, wheat straw stalk efficient degradation composite bacteria is made up of carrier and composite fungus agent, carrier is the corn cob powder, and composite fungus agent is formed with the II microbial inoculum by I number; Its parts by weight are: I microbial inoculum 80-90 part; II microbial inoculum 10-20 part; The I microbial inoculum by in genus bacillus or the pseudomonas at least a kind form; The II microbial inoculum by in streptomycete or the Zymomonas mobilis at least a kind form.
And, described genus bacillus by in subtilis or Bacillus licheniformis or bacillus acidocldarius or the bacillus polymyxa at least 3 kinds form;
Pseudomonas is a Pseudomonas stutzeri;
Streptomycete is a streptomyces albus;
Zymomonas mobilis is a zymomonas mobilis.
The preparation method of this wheat straw stalk efficient degradation composite bacteria fertilizer may further comprise the steps:
(1) respectively behind corresponding inoculation of medium different strain, equal 160r/min in 45 ℃ of shaking tables, shaking culture 24-72h cultivates the back dilution that finishes and forms bacterium liquid, and making every milliliter of bacterium number is 400,000,000, i.e. 400,000,000/ml;
(2) different bacterium liquid are adsorbed onto on the carrier by the bacterium number of every gram carrier 200,000,000 respectively, mix respectively according to the composition of I microbial inoculum with the II microbial inoculum, promptly constitute I microbial inoculum and II microbial inoculum;
(3) the wheat straw stalk is crushed to 0.5-0.8cm through disintegrating apparatus and forms the broken end of wheat straw stalk, get I microbial inoculum and II microbial inoculum by 0.1% of the broken last weight of wheat straw stalk, wherein the I microbial inoculum accounts for 80-90%, the II microbial inoculum accounts for 10-20%, in the broken end of wheat straw stalk, add the I microbial inoculum, keep the broken last humidity 50-60% of wheat straw stalk; Behind good oxygen condition bottom fermentation 1-2d, add the II microbial inoculum, keep the broken last humidity 40-55% of wheat straw stalk, can make wheat straw stalk efficient degradation composite bacteria fertilizer behind the broken end fermentation of the wheat straw stalk 4-5d.
Advantage of the present invention and beneficial effect are:
1. the present invention adopts microbiobacterial agent cooperative fermentation degraded wheat straw stalk, and different microorganisms is secreted complementary enzyme in degraded, substrate is fully degraded, thereby improves the efficient of industrial fermentation process; The single microorganism fermentation has produces the incomplete phenomenon of enzyme, and promptly the enzyme that is produced is the part enzyme in the required enzyme system of degraded, and the incomplete phenomenon of degraded takes place.
2. the microbiobacterial agent of the present invention's employing can shorten degradation time.Add fermenting agent of the present invention during the fermentation, make the time of producing fertilizer shorten to 4-5d.Than time that single culture fermentation or other flora fermentation process are consumed (organic fertilizer production 〉=7d), the present invention has shortened degradation cycle more than 40%, not only simplify production technique, improved usage ratio of equipment, and reduced reaction cost effectively more than 20%.
3. equipment required for the present invention is simple, and technology is easy, has realized the harmless treatment of wheat straw stalk, produces the fertilizer that can improve the soil simultaneously, has solved current wheat straw stalk and has been difficult to the difficult problem of degrading, utilizing, has extremely far-reaching social effect and economic worth.
Embodiment
The present invention is described in further detail by following examples, but the technology contents that present embodiment is narrated is illustrative, rather than determinate, should not limit to protection scope of the present invention according to this.
Embodiment 1:
A kind of wheat straw stalk efficient degradation composite bacteria fertilizer, this wheat straw stalk efficient degradation composite bacteria fertilizer is to be formed by the broken end of wheat straw stalk efficient degradation composite bacteria fermentation wheat straw stalk, wheat straw stalk efficient degradation composite bacteria is made up of carrier and composite fungus agent, carrier is the corn cob powder, composite fungus agent is formed with the II microbial inoculum by I number, its parts by weight are: 80 parts of I microbial inoculums, 20 parts of II microbial inoculums.
Wherein, I microbial inoculum composition and kind umber are: 20 parts of subtilises, 35 parts of Bacillus licheniformis, 20 parts of bacillus polymyxa, 25 parts of Pseudomonas stutzeris.
The II microbial inoculum is formed and the kind umber is: 40 parts of streptomyces albus, 60 parts of zymomonas mobilis.
The preparation method of this wheat straw stalk efficient degradation composite bacteria fertilizer may further comprise the steps:
(1) respectively behind corresponding inoculation of medium different strain, equal 160r/min in 45 ℃ of shaking tables, shaking culture 36h cultivates the back dilution that finishes and forms bacterium liquid, and making every milliliter of bacterium number is 400,000,000, i.e. 400,000,000/ml;
Substratum is:
Genus bacillus, pseudomonas, Zymomonas mobilis, streptomycete culture medium are: yeast extract 2.0g/L, glucose 8.0g/L, dipotassium hydrogen phosphate 6.5g/L, potassium primary phosphate 4.0g/L, SODIUMNITRATE 3.0g/L, ammonium chloride 1.0g/L, sodium-chlor 3.0g/L, sal epsom 0.05g/L, manganous sulfate 0.01g/L, ferrous sulfate 0.005g/L, pH7.5.
(2) different bacterium liquid are adsorbed onto on the carrier by the bacterium number of every gram carrier 200,000,000 respectively, mix respectively according to the composition of I microbial inoculum with the II microbial inoculum, promptly constitute I microbial inoculum and II microbial inoculum;
(3) the wheat straw stalk is crushed to 0.8cm through disintegrating apparatus and forms the broken end of wheat straw stalk, get I microbial inoculum and II microbial inoculum by 0.1% of the broken last weight of wheat straw stalk, wherein the I microbial inoculum accounts for 80%, and the II microbial inoculum accounts for 20%, in the broken end of wheat straw stalk, add the I microbial inoculum, keep the broken last humidity 50% of wheat straw stalk; Behind good oxygen condition bottom fermentation 1d, add the II microbial inoculum, keep the broken last humidity 40% of wheat straw stalk, can make wheat straw stalk efficient degradation composite bacteria fertilizer behind the broken end fermentation of the wheat straw stalk 4d.
Embodiment 2:
A kind of wheat straw stalk efficient degradation composite bacteria fertilizer, this wheat straw stalk efficient degradation composite bacteria fertilizer is to be formed by the broken end of wheat straw stalk efficient degradation composite bacteria fermentation wheat straw stalk, wheat straw stalk efficient degradation composite bacteria is made up of carrier and composite fungus agent, carrier is the corn cob powder, composite fungus agent is formed with the II microbial inoculum by I number, its parts by weight are: 83 parts of I microbial inoculums, 17 parts of II microbial inoculums.
Wherein, I microbial inoculum composition and kind umber are: 35 parts of subtilises, 38 parts of Bacillus licheniformis, 27 parts of bacillus acidocldariuses.
The II microbial inoculum is formed and the kind umber is: 100 parts of streptomyces albus.
The preparation method of this wheat straw stalk efficient degradation composite bacteria fertilizer may further comprise the steps:
(1) respectively behind corresponding inoculation of medium different strain, equal 160r/min in 45 ℃ of shaking tables, shaking culture 48h cultivates the back dilution that finishes and forms bacterium liquid, and making every milliliter of bacterium number is 400,000,000, i.e. 400,000,000/ml;
Genus bacillus, streptomycete culture medium are: yeast extract 2.0g/L, glucose 8.0g/L, dipotassium hydrogen phosphate 6.5g/L, potassium primary phosphate 4.0g/L, SODIUMNITRATE 3.0g/L, ammonium chloride 1.0g/L, sodium-chlor 3.0g/L, sal epsom 0.05g/L, manganous sulfate 0.01g/L, ferrous sulfate 0.005g/L, pH7.5.
(2) different bacterium liquid are adsorbed onto on the carrier by the bacterium number of every gram carrier 200,000,000 respectively, mix respectively according to the composition of I microbial inoculum with the II microbial inoculum, promptly constitute I microbial inoculum and II microbial inoculum;
(3) the wheat straw stalk is crushed to 0.5cm through disintegrating apparatus and forms the broken end of wheat straw stalk, get I microbial inoculum and II microbial inoculum by 0.1% of the broken last weight of wheat straw stalk, wherein the I microbial inoculum accounts for 83%, and the II microbial inoculum accounts for 17%, in the broken end of wheat straw stalk, add the I microbial inoculum, keep the broken last humidity 55% of wheat straw stalk; Behind good oxygen condition bottom fermentation 2d, add the II microbial inoculum, keep the broken last humidity 45% of wheat straw stalk, can make wheat straw stalk efficient degradation composite bacteria fertilizer behind the broken end fermentation of the wheat straw stalk 5d.
Embodiment 3:
A kind of wheat straw stalk efficient degradation composite bacteria fertilizer, this wheat straw stalk efficient degradation composite bacteria fertilizer is to be formed by the broken end of wheat straw stalk efficient degradation composite bacteria fermentation wheat straw stalk, wheat straw stalk efficient degradation composite bacteria is made up of carrier and composite fungus agent, carrier is the corn cob powder, composite fungus agent is formed with the II microbial inoculum by I number, its parts by weight are: 90 parts of I microbial inoculums, 10 parts of II microbial inoculums.
Wherein, I microbial inoculum composition and kind umber are: 25 parts of Bacillus licheniformis, 35 parts of bacillus acidocldariuses, 15 parts of bacillus polymyxa, 25 parts of Pseudomonas stutzeris.
The II microbial inoculum is formed and the kind umber is: 55 parts of streptomyces albus, 45 parts of zymomonas mobilis.
Carrier is made of the corn cob powder.
The preparation method of this wheat straw stalk efficient degradation composite bacteria fertilizer may further comprise the steps:
(1) respectively behind corresponding inoculation of medium different strain, equal 160r/min in 45 ℃ of shaking tables, shaking culture 72h cultivates the back dilution that finishes and forms bacterium liquid, and making every milliliter of bacterium number is 400,000,000, i.e. 400,000,000/ml;
Substratum is:
Genus bacillus, pseudomonas, Zymomonas mobilis, streptomycete culture medium are: yeast extract 2.0g/L, glucose 8.0g/L, dipotassium hydrogen phosphate 6.5g/L, potassium primary phosphate 4.0g/L, SODIUMNITRATE 3.0g/L, ammonium chloride 1.0g/L, sodium-chlor 3.0g/L, sal epsom 0.05g/L, manganous sulfate 0.01g/L, ferrous sulfate 0.005g/L, pH7.5.
(2) different bacterium liquid are adsorbed onto on the carrier by the bacterium number of every gram carrier 200,000,000 respectively, mix respectively according to the composition of I microbial inoculum with the II microbial inoculum, promptly constitute I microbial inoculum and II microbial inoculum;
(3) the wheat straw stalk is crushed to 0.6cm through disintegrating apparatus and forms the broken end of wheat straw stalk, get I microbial inoculum and II microbial inoculum by 0.1% of the broken last weight of wheat straw stalk, wherein the I microbial inoculum accounts for 90%, and the II microbial inoculum accounts for 10%, in the broken end of wheat straw stalk, add the I microbial inoculum, keep the broken last humidity 65% of wheat straw stalk; Behind good oxygen condition bottom fermentation 1d, add the II microbial inoculum, keep the broken last humidity 55% of wheat straw stalk, can make wheat straw stalk efficient degradation composite bacteria fertilizer behind the broken end fermentation of the wheat straw stalk 4d.
Claims (3)
1. wheat straw stalk efficient degradation composite bacteria fertilizer, it is characterized in that: this wheat straw stalk efficient degradation composite bacteria fertilizer is to be formed by the broken end of wheat straw stalk efficient degradation composite bacteria fermentation wheat straw stalk, wheat straw stalk efficient degradation composite bacteria is made up of carrier and composite fungus agent, carrier is the corn cob powder, and composite fungus agent is formed with the II microbial inoculum by I number; Its parts by weight are: I microbial inoculum 80-90 part; II microbial inoculum 10-20 part; The I microbial inoculum by in genus bacillus or the pseudomonas at least a kind form; The II microbial inoculum by in streptomycete or the Zymomonas mobilis at least a kind form.
2. wheat straw stalk efficient degradation composite bacteria fertilizer according to claim 1 is characterized in that: described genus bacillus by in subtilis or Bacillus licheniformis or bacillus acidocldarius or the bacillus polymyxa at least 3 kinds form;
Pseudomonas is a Pseudomonas stutzeri;
Streptomycete is a streptomyces albus;
Zymomonas mobilis is a zymomonas mobilis.
3. the preparation method of a wheat straw stalk efficient degradation composite bacteria fertilizer as claimed in claim 1 or 2, it is characterized in that: this method may further comprise the steps:
(1) respectively behind corresponding inoculation of medium different strain, equal 160r/min in 45 ℃ of shaking tables, shaking culture 24-72h cultivates the back dilution that finishes and forms bacterium liquid, and making every milliliter of bacterium number is 400,000,000, i.e. 400,000,000/ml;
(2) different bacterium liquid are adsorbed onto on the carrier by the bacterium number of every gram carrier 200,000,000 respectively, mix respectively according to the composition of I microbial inoculum with the II microbial inoculum, promptly constitute I microbial inoculum and II microbial inoculum;
(3) the wheat straw stalk is crushed to 0.5-0.8cm through disintegrating apparatus and forms the broken end of wheat straw stalk, get I microbial inoculum and II microbial inoculum by 0.1% of the broken last weight of wheat straw stalk, wherein the I microbial inoculum accounts for 80-90%, the II microbial inoculum accounts for 10-20%, in the broken end of wheat straw stalk, add the I microbial inoculum, keep the broken last humidity 50-60% of wheat straw stalk; Behind good oxygen condition bottom fermentation 1-2d, add the II microbial inoculum, keep the broken last humidity 40-55% of wheat straw stalk, can make wheat straw stalk efficient degradation composite bacteria fertilizer behind the broken end fermentation of the wheat straw stalk 4-5d.
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| CN102286361A (en) * | 2011-07-13 | 2011-12-21 | 南开大学 | Biomass fermentation reaction device and application |
| CN102503608A (en) * | 2011-09-29 | 2012-06-20 | 南开大学 | Method for preparing organic fertilizer from agricultural wastes |
| CN102765972A (en) * | 2012-07-17 | 2012-11-07 | 上海交通大学 | Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent |
| US20140352376A1 (en) * | 2013-05-28 | 2014-12-04 | BiOWiSH Technologies, Inc. | Fertilizer compositions methods of making and using same |
| CN104973897A (en) * | 2015-07-29 | 2015-10-14 | 武汉领潮生物科技有限公司 | Straw organic fertilizer |
| CN105669321A (en) * | 2016-01-07 | 2016-06-15 | 扬州国本生物科技有限公司 | Bio-organic fertilizer with plant straw as substrate and preparation method thereof |
| CN105924246A (en) * | 2016-04-25 | 2016-09-07 | 上海交通大学 | Preparation method for biological fermentation integrated organic fertilizer |
| CN107082656A (en) * | 2017-04-01 | 2017-08-22 | 南开大学 | A kind of method for carrying out producing acid treatment to stalk with aerobic acid-producing bacteria |
| CN110938574A (en) * | 2019-12-27 | 2020-03-31 | 黑龙江省农业科学院耕作栽培研究所 | Corn straw decomposition microbial inoculum |
| CN111117934A (en) * | 2020-01-21 | 2020-05-08 | 南京工业大学 | Pseudomonas stutzeri and application thereof |
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| CN111117934A (en) * | 2020-01-21 | 2020-05-08 | 南京工业大学 | Pseudomonas stutzeri and application thereof |
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Application publication date: 20110420 |