CN102018711A - Application of chelerythrine in preparing medicine for killing aquatic animal ectoparasites and preventer - Google Patents
Application of chelerythrine in preparing medicine for killing aquatic animal ectoparasites and preventer Download PDFInfo
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- CN102018711A CN102018711A CN2010105651424A CN201010565142A CN102018711A CN 102018711 A CN102018711 A CN 102018711A CN 2010105651424 A CN2010105651424 A CN 2010105651424A CN 201010565142 A CN201010565142 A CN 201010565142A CN 102018711 A CN102018711 A CN 102018711A
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Abstract
The invention provides application of chelerythrine for preparing a medicine for killing aquatic animal ectoparasites and a preventer, belonging to the technical field of application of chemical products. The preventer is characterized by comprising the following materials in percentage by weight: 5%-25% of the chelerythrine, 5%-10% of a surface active agent, 10%-20% of a lytic agent, and the balance of solvent, wherein the surface active agent is Tween-20; the lytic agent is dimethyl sulfoxide; and the solvent is absolute ethyl alcohol. The chelerythrine has good effect of killing the aquatic animal ectoparasites, is low in toxicity, low in residue, low in toxicity to the nontarget organisms and free of pollution to the environment, and is safe and reliable in use. The chelerythrine extracted from the natural plants is utilized as the active ingredient of the preventer, so that the preparation method is simple, the production cost is low and the application method is simple; and the preventer is directly splashed into ponds based on the dosage of 1-2 mL/m<3> to effectively prevent or kill the aquatic animal ectoparasites, and has good prevention or killing effects.
Description
Technical field
The invention belongs to chemical products purposes technical field, be specially chelerythrine and kill the application in the aquatic animal vermin medicine and prevent and treat agent in preparation.
Background technology
Chelerythrine is that its structural formula is from a kind of chemical compound of the whole herb with root extraction separation of bloodroot Herba Chelidonii (Chelidonium majus L.):
The molecular formula of this chemical compound is C
21H
18NO
4, molecular weight is 348, is pale yellow powder or shallow white crystal, and free alkali is colourless, and its quaternary ammonium salt is xanchromatic.The aqueous solution of free alkali and salt shows violet fluorescence, and is easily molten in organic solvents such as chloroform, methanol, ethanol, indissoluble in the petroleum ether, fusing point 195-205 ℃.Application in this chemical compound actual production is less.
Killing parasite at present on the Aquatic product mainly is to transplant people's medicine, veterinary drug and part pesticide, as killing parasite with chemicalses such as metrifonate, avilamycin, ivermectins, though certain effect is arranged, but what life-time service brought is a series of problems such as environmental pollution, drug residue, drug resistance, also becomes the Green Trade Barrier problem of present food security and product water outlet.Chelerythrine is the medicine from extracted form natural plant, have low toxicity, low-residual, poison lower non-target organism, advantages such as environmentally safe, " the medicine public hazards " that can reduce or avoid present chemicals to bring are the development trend and a big focus of Aquatic product disease control.
Summary of the invention
At the above-mentioned problems in the prior art, the objective of the invention is to design the technical scheme that provides a kind of chelerythrine to kill the application in the aquatic animal vermin medicine and prevent and treat agent in preparation, its insect killing effect is good, and also have low toxicity, low-residual, lower, advantages such as environmentally safe to the non-target organism murder by poisoning.
Described chelerythrine is killed application in the aquatic animal vermin medicine in preparation.
Described chelerythrine is killed application in the external little melonworm of aquatic animal, dactylogyrider, the Trichodina medicine in preparation.
A kind of external parasite preventing and treating agent for aquatic product animals of killing of described chelerythrine preparation is characterized in that being made up of the following material of weight percentage: chelerythrine 5%-25%, and surfactant 5%-10%, lytic agent 10%-20%, surplus is a solvent; Described surfactant is a tween 20, and described lytic agent is a dimethyl sulfoxide, and described solvent is a dehydrated alcohol.
Described a kind of external parasite preventing and treating agent for aquatic product animals of killing, the weight percentage that it is characterized in that described chelerythrine is 10%-20%, preferred 15%-18%.
Described a kind of external parasite preventing and treating agent for aquatic product animals of killing, the weight percentage that it is characterized in that described surfactant is 6%-8%.
Described a kind of external parasite preventing and treating agent for aquatic product animals of killing, the weight percentage that it is characterized in that described lytic agent is 15%-18%.
Described a kind of preparation method of killing external parasite preventing and treating agent for aquatic product animals is characterized in that may further comprise the steps:
1) according to the above-mentioned substance ratio, take by weighing chelerythrine, surfactant, lytic agent, solvent, standby;
2) chelerythrine and lytic agent are mixed to dissolving fully;
3) surfactant, solvent are added step 2) stir in the mixed solution of gained, promptly get and kill external parasite preventing and treating agent for aquatic product animals.
Above-mentioned chelerythrine is killed application in the aquatic animal vermin medicine in preparation, and insect killing effect is good, and also have low toxicity, low-residual, non-target organism is poisoned lower, advantages such as environmentally safe, safe and reliable.This control agent is an effective ingredient with the chelerythrine of extracted form natural plant, and preparation method is simple, and production cost is low, and using method is simple, directly presses 1-2 mL/m
3Dosage splash and in the pond, can effectively prevent and treat or kill the aquatic animal vermin, control or killing effect are good.
The percentage composition that relates in the present patent application, unless otherwise indicated, all the other are weight percentage.
The specific embodiment
Now in conjunction with embodiments of the invention, the present invention is described in detail, so that advantage of the present invention is more obvious, and by test explanation beneficial effect of the present invention.
Embodiment 1
Take by weighing chelerythrine 5g, stirring and dissolving in the 10g dimethyl sulfoxide takes by weighing 5g surfactant tween 20, etoh solvent 80g, and mixing and stirring promptly gets prevents and treats agent.
Embodiment 2
Take by weighing chelerythrine 10g, stirring and dissolving in the 20g dimethyl sulfoxide takes by weighing 10 g surfactant tween 20s, etoh solvent 60g, and mixing and stirring promptly gets prevents and treats agent.
Embodiment 3
Take by weighing chelerythrine 15g, stirring and dissolving in the 15g dimethyl sulfoxide takes by weighing 6g surfactant tween 20, etoh solvent 64g, and mixing and stirring promptly gets prevents and treats agent.
Embodiment 4
Take by weighing chelerythrine 20g, stirring and dissolving in the 12g dimethyl sulfoxide takes by weighing 7g surfactant tween 20, etoh solvent 61g, and mixing and stirring promptly gets prevents and treats agent.
Embodiment 5
Take by weighing chelerythrine 25g, stirring and dissolving in the 18g dimethyl sulfoxide takes by weighing 8g surfactant tween 20, etoh solvent 49g, and mixing and stirring promptly gets prevents and treats agent.
The foregoing description can be enumerated a lot, and this differs and one enumerates again, so long as select according to the parameter area of technical scheme of the present invention, can prepare the aquatic animal parasite control agent that reaches beneficial effect of the present invention.Above-mentioned control agent can directly be splashed in the pond, and using dosage is: 1 ~ 2 mL/m
3
Below make chelerythrine chloride and above-mentioned control agent clear to verminal control of aquatic animal and killing effect by test.
Test 1: chelerythrine is killed the test of pesticide effectiveness of the little melonworm of parasitic Fish
1. materials and methods
1.1 test fish
(8.7 ± 0.3cm) from Zhejiang Institute of Fresh Water Aquatic Products seed base for the Ctenopharyngodon idellus of the little melonworm of severe infections.The test fish is supported 7 d temporarily in cement pit after, picked at random 10 tail fishes are got the holobranch microscopy and observe little melonworm quantity on the statistics gill, and the little melonworm infection rate of test fish is 100%, and every tail fish on average infects about 70 of little melonworm.Chelerythrine lytic agent dmso solution.
1.2. external insecticidal test
In vitro tests is carried out on 24 porocyte culture plates, and the Ctenopharyngodon idellus holobranch of the little melonworm of severe infections is put into the plate that fills distilled water, treat that little melonworm is swum out of in the gill after, with suction pipe the little melonworm of maturation is collected that to put into another plate standby.Medicinal liquid (0.2,0.3,0.4,0.5,0.6,0.7,0.8 and 0.9 mL/m that in each cell hole, adds about 2ml variable concentrations
3) and 20 little melonworm adults, per hour microscopy is observed once, the little melonworm mortality rate in each hole of statistics behind the 4h.Test is provided with DMSO matched group and fully sudden and violent gas nature water matched group, every group of test triplicate.
The dead criterion of little melonworm: the polypide cilium does not move, and kytoplasm does not flow, and after birth breaks, the nucleus fragmentation.
1.3 insecticidal test in the body
The insecticidal test concentration that chelerythrine is set according to external insecticidal test is 0.2,0.3,0.4,0.5,0.6,0.7,0.8 and 0.9 mL/m
3Contain the natural water of abundant aeration in volume is the glass jar of 80cm * 60cm * 40cm, pH 7.0～7.5,25 ± 1 ℃ of water temperatures.In water body, add the chelerythrine of variable concentrations according to the concentration that configures, and stir.Each glass jar is thrown in and is infected Ctenopharyngodon idellus 10 tails that little melonworm is arranged, and gets the film-making of Ctenopharyngodon idellus holobranch, microscopy behind medication 48 h, adds up every tail Ctenopharyngodon idellus in gill portion little melonworm survival quantity, calculates average killing rate, and test repeats 2 times, and carries out the DMSO controlled trial simultaneously.
The little melonworm of killing rate (%)=(the little melonworm of matched group on average survive the little melonworm of quantity-test group on average survive quantity)/matched group quantity * 100 % of on average surviving
Fish quantity * 100% of fish average mortality (%)=(the fish survival quantity during the fish quantity of input-microscopy)/throw in
The criterion of little melonworm death is: the polypide cilium does not move, and kytoplasm does not flow, and after birth breaks, the nucleus fragmentation.
1.4 date processing
Test data is handled with SPSS16.0 statistics software, all parameters all use mean ± standard deviation (
± SD) expression.
2. result of the test
2.1 chelerythrine external kills little melonworm test
Result of the test shows: chelerythrine has stronger killing action to little melonworm, and along with its killing action of increase of concentration strengthens gradually, its concentration is 0.9 mL/m
3The time, be 100% to the killing rate of little melonworm, the LC of its 4h
50And LC
90Be respectively 0.437,0.853 mL/m
3
External insecticidal test result also shows: chelerythrine has great destruction to the structure of little melonworm.After the 4h effect, the cell membrane of little melonworm destroys fully, the kytoplasm outflow, and nucleus is invisible, the cilium stop motion.And the little melonworm structure of matched group is intact, the ciliary movement freedom, and macronucleus is high-visible, and this may be the parasiticidal main mechanism of action of chelerythrine.
2.2 insecticidal test in the body of chelerythrine
Result of the test shows: chelerythrine has stronger killing action to little melonworm, and its concentration is 0.2,0.3,0.4,0.5,0.6,0.7,0.8 and 0.9 mL/m
3The time, the killing rate of the little melonworm on the parasitic Ctenopharyngodon idellus is respectively: 16.1%, 17.3%, 32.9%, 53.9%, 75.3%, 82.3%, 89.4% and 96.8%.0.2,0.3,0.4 mL/m
3The Ctenopharyngodon idellus mortality rate of concentration group is 20%, 0.6 mL/m
3The Ctenopharyngodon idellus mortality rate of concentration group is 10%, and the Ctenopharyngodon idellus mortality rate of matched group is 40%, and all the other test group mortality rates are 0, and the result shows that chelerythrine has the test fishing gear of little melonworm that stronger protective effect is arranged to infection.
Test 2: chelerythrine is killed the test of pesticide effectiveness of parasitic Fish dactylogyrider
1.1 test fish
(5.7 ± 0.2cm) available from Changzhou Zhai Qiao for the Carassius auratus of severe infections dactylogyrider.And according to the ratio about 1:5 with do not infect parasitic Carassius auratus and raise together with infection, infect after 15-20 days, picked at random 10 tail fishes are got the holobranch microscopy and observe dactylogyrider quantity on the statistics gill, test fish dactylogyrider infection rate is 100%, and every tail fish on average infects about 50 of dactylogyrider.Chelerythrine lytic agent dmso solution.
1.2 effective drug determination of activity
The insecticidal activity assay method, carry out according to methods such as Wang Gaoxue etc. and Miguel Rubio-Godoy: during the test of pesticide effectiveness, contain the subsoil water of abundant aeration in the glass jar of certain volume, pH 7.0～7.5,25 ± 1 ℃ of water temperatures.In water body, add each sample of aforementioned preparation according to the concentration that configures, and stir.Each glass jar is thrown in and is infected Carassius auratus 20 tails that dactylogyrider is arranged, and the film-making of depletion fish holobranch, microscopy when 48 h are added up every tail Carassius auratus in gill portion dactylogyrider survival quantity, calculate average killing rate, and carry out controlled trial simultaneously.
The dactylogyrider of killing rate (%)=(dactylogyrider of matched group on average survive the dactylogyrider of quantity-test group on average survive quantity)/matched group quantity * 100 % of on average surviving
Fish quantity * 100% of fish average mortality (%)=(the fish survival quantity during the fish quantity of input-microscopy)/throw in
The highest valid density of sample, the i.e. pairing solvent extraction substrate concentration of the highest killing rate.
2. result of the test shows: chelerythrine has stronger killing action to dactylogyrider, and its concentration is 0.2,0.3,0.4,0.5,0.6,0.7,0.8 and 0.9 mL/m
3The time, the killing rate of the dactylogyrider on the parasitic Ctenopharyngodon idellus is respectively: 15.3%, 16.7%, 35.4%, 57.9%, 77.5%, 86.2%, 90.6% and 100%.0.2,0.3,0.4 mL/m
3The Ctenopharyngodon idellus mortality rate of concentration group is 20%, 0.5 mL/m
3The Ctenopharyngodon idellus mortality rate of concentration group is 10%, and the Ctenopharyngodon idellus mortality rate of matched group is 30%, and all the other test group mortality rates are 0, and the result shows that chelerythrine has the test fishing gear of dactylogyrider that stronger protective effect is arranged to infection.
Test 3: chelerythrine is killed the test of pesticide effectiveness of parasitic Fish Trichodina
1. materials and methods
1.1 test fish
(8.7 ± 0.3cm) from Zhejiang Institute of Fresh Water Aquatic Products seed base for the Carassius auratus of severe infections Trichodina.The test fish is supported 7 d temporarily in cement pit after, picked at random 10 tail fishes are got the holobranch microscopy and observe Trichodina quantity on the statistics gill, and test fish Trichodina infection rate is 100%, and every tail fish on average infects about 70 of Trichodina.Chelerythrine lytic agent dmso solution.
1.2 the parasite killing test of pesticide effectiveness
The insecticidal test concentration that chelerythrine is set according to trial test is 0.2,0.3,0.4,0.5,0.6,0.7,0.8 and 0.9 mL/m
3Contain the natural water of abundant aeration in volume is the glass jar of 80cm * 60cm * 40cm, pH 7.0～7.5,25 ± 1 ℃ of water temperatures.In water body, add the chelerythrine of variable concentrations according to the concentration that configures, and stir.Each glass jar is thrown in and is infected Carassius auratus 10 tails that Trichodina is arranged, and the film-making of depletion fish holobranch, microscopy behind medication 48 h are added up every tail Carassius auratus in gill portion Trichodina survival quantity, calculate average killing rate, and test repeats 2 times, and carries out the DMSO controlled trial simultaneously.
The Trichodina of killing rate (%)=(Trichodina of matched group on average survive the Trichodina of quantity-test group on average survive quantity)/matched group quantity * 100 % of on average surviving
Fish quantity * 100% of fish average mortality (%)=(the fish survival quantity during the fish quantity of input-microscopy)/throw in
The criterion of Trichodina death is: the polypide cilium does not move, and kytoplasm does not flow, and after birth breaks, the nucleus fragmentation.
2. result of the test shows: chelerythrine has stronger killing action to Trichodina, and its concentration is 0.2,0.3,0.4,0.5,0.6,0.7 and 0.8 mL/m
3The time, the killing rate of the Trichodina on the parasitic Ctenopharyngodon idellus is respectively: 24.8%, 37.7%, 52.9%, 66.4%, 85.6%, 95.4%, 97.4% and 100%.0.2,0.3,0.4 mL/m
3The Ctenopharyngodon idellus mortality rate of concentration group is 20%, and the Ctenopharyngodon idellus mortality rate of matched group is 40%, and all the other test group mortality rates are 0, and the result shows that chelerythrine has the test fishing gear of Trichodina that stronger protective effect is arranged to infection.
Above-mentioned test 1-3 shows: chelerythrine is obvious to verminal control of aquatic animal and killing effect.As effective ingredient, promptly make above-mentioned described control agent behind the adding auxiliary agent with chelerythrine.
Test 4: above-mentioned control agent sees Table 1-3 to verminal control of aquatic animal and killing effect test.
Result of the test shows: above-mentioned control agent is obvious to verminal control of aquatic animal and killing effect.
Claims (7)
1. chelerythrine is killed application in the aquatic animal vermin medicine in preparation.
2. chelerythrine as claimed in claim 1 is killed application in the external little melonworm of aquatic animal, dactylogyrider, the Trichodina medicine in preparation.
3. a kind of external parasite preventing and treating agent for aquatic product animals of killing of chelerythrine preparation according to claim 1, it is characterized in that forming: chelerythrine 5%-25% by the following material of weight percentage, surfactant 5%-10%, lytic agent 10%-20%, surplus is a solvent; Described surfactant is a tween 20, and described lytic agent is a dimethyl sulfoxide, and described solvent is a dehydrated alcohol.
4. will remove 3 described a kind of external parasite preventing and treating agent for aquatic product animals of killing as right, the weight percentage that it is characterized in that described chelerythrine is 10%-20%, preferred 15%-18%.
5. will remove 3 described a kind of external parasite preventing and treating agent for aquatic product animals of killing as right, the weight percentage that it is characterized in that described surfactant is 6%-8%.
6. will remove 3 described a kind of external parasite preventing and treating agent for aquatic product animals of killing as right, the weight percentage that it is characterized in that described lytic agent is 15%-18%.
7. to go 3 described a kind of preparation methoies of killing external parasite preventing and treating agent for aquatic product animals as right, it is characterized in that may further comprise the steps:
1) according to the above-mentioned substance ratio, take by weighing chelerythrine, surfactant, lytic agent, solvent, standby;
2) chelerythrine and lytic agent are mixed to dissolving fully;
3) surfactant, solvent are added step 2) stir in the mixed solution of gained, promptly get and kill external parasite preventing and treating agent for aquatic product animals.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397034A (en) * | 2014-11-12 | 2015-03-11 | 苏州市相城区盛胡特种养殖专业合作社 | Environment-friendly type pesticide for aquatic products and preparation method thereof |
| CN105031128A (en) * | 2015-09-18 | 2015-11-11 | 武汉华扬动物药业有限责任公司 | Traditional Chinese medicine composition for preventing or treating ectoparasite of aquatic culture animals and preparation method and application thereof |
| CN108653261A (en) * | 2018-05-04 | 2018-10-16 | 浙江省淡水水产研究所 | Application of the 6- n-pentyloxies Rhein in prevention saprolegniasis and ich |
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| CN101099738A (en) * | 2007-01-10 | 2008-01-09 | 西北农林科技大学 | Application of sanguinarine in preparing aquatic animal medicine and method for preparing its preparation |
| CN101209043A (en) * | 2006-12-27 | 2008-07-02 | 长沙世唯科技有限公司 | Application of sanguinarine or toddaline in prevention and cure of schistosomiasis |
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Patent Citations (2)
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| CN101209043A (en) * | 2006-12-27 | 2008-07-02 | 长沙世唯科技有限公司 | Application of sanguinarine or toddaline in prevention and cure of schistosomiasis |
| CN101099738A (en) * | 2007-01-10 | 2008-01-09 | 西北农林科技大学 | Application of sanguinarine in preparing aquatic animal medicine and method for preparing its preparation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397034A (en) * | 2014-11-12 | 2015-03-11 | 苏州市相城区盛胡特种养殖专业合作社 | Environment-friendly type pesticide for aquatic products and preparation method thereof |
| CN105031128A (en) * | 2015-09-18 | 2015-11-11 | 武汉华扬动物药业有限责任公司 | Traditional Chinese medicine composition for preventing or treating ectoparasite of aquatic culture animals and preparation method and application thereof |
| CN108653261A (en) * | 2018-05-04 | 2018-10-16 | 浙江省淡水水产研究所 | Application of the 6- n-pentyloxies Rhein in prevention saprolegniasis and ich |
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| CN102018711B (en) | 2013-10-30 |
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