CN101999554B - Special animal nutrient for milk cows and preparation method thereof - Google Patents
Special animal nutrient for milk cows and preparation method thereof Download PDFInfo
- Publication number
- CN101999554B CN101999554B CN2010105924830A CN201010592483A CN101999554B CN 101999554 B CN101999554 B CN 101999554B CN 2010105924830 A CN2010105924830 A CN 2010105924830A CN 201010592483 A CN201010592483 A CN 201010592483A CN 101999554 B CN101999554 B CN 101999554B
- Authority
- CN
- China
- Prior art keywords
- weight
- bitter
- fulvic acid
- fermentation culture
- bean pulp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to a special animal nutrient for milk cows and a preparation method thereof. The special animal nutrient for milk cows comprises the following components in percentage by weight: 40-60% of fulvic acid, 0.2-0.5% of taraxacum total flavone and 39.5-59.5% of fenugreek pulp fermented culture. The preparation method comprises the following steps: extracting fulvic acid; preparing fenugreek pulp fermented culture; and evenly mixing 40-60% of fulvic acid, 0.2-0.5% of taraxacum total flavone and 39.5-59.5% of fenugreek pulp fermented culture. The special animal nutrient for milk cows has obvious effects of improving the gastrointestinal function, enhancing the immunity of the organism, lowering the morbidity of mastitis and increasing the milk yield, has the advantages of high safety and convenience as well as stable performance, and is easy to store.
Description
Technical field
The present invention relates to a kind of animal nutrient and preparation method thereof, particularly relate to a kind of special animal nutrient for milk cows and preparation method thereof.
Background technology
In raising dairy cattle; all kinds of mammitises of milk cow are having a strong impact on the development of milk cattle cultivating industry always; after milk cow suffered from mammitis, the output of milk approximately descended about 20%, and therefore various types of mammitises are that the incidence of disease is high, popular soon, large a kind of disease type causes damage.At present the whole world approximately has 1/3 milk cow to suffer from various types of mammitises and recessive mastitis, particularly China, and the incidence of disease of mastitis for milk cows generally is higher than abroad, and illness rate can be up to more than 50%.
Since the forties in last century, antibiotic came out, antibiotic was the essential drugs of all kinds of mammitises of control milk cow always, and has brought into play indelible magical effect within one period.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body, can be distributed to blood circulation each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone, antibiotic can cause antigenic quality to reduce, directly affect immunologic process, thereby the inoculation to vaccine produces harmful effect, and the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can affect simultaneously in the body profitable strain growth acting on pathogen, especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys, in vivo the harmful bacteria of the hiding amount reproduction of taking advantage of the occasion, and cause endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroying microecological balance, and another or multiple endogenous or external source germ immediately reinfect body are caused.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most antibiotics is excreted by ight soil, also can remain in the milk by the lactation process.After being excreted in the environment, the stable antibiotic of properties still can have a very long time, thereby cause the medicament residue in the environment, these remaining medicines are slowly accumulated in human body and other plant body by livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium, lose the resistance to some disease, or because accumulating in a large number body is produced toxic action.
Summary of the invention
The purpose of this invention is to provide a kind of special animal nutrient for milk cows and preparation method thereof, overcome effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.
For achieving the above object, the invention provides a kind of special animal nutrient for milk cows, comprise the constituent of following percentage by weight: fulvic acid 40%-60%, Total Flavonoids from Taraxacum mongolicum 0.2%-0.5% and bitter bean pulp fermentation culture 39.5%-59.5%.
On the basis of technique scheme, described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, S. cervisiae, bacillus subtilis and bacillus licheniformis.
Further, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90%-97%, corn flour 2%-8% and beet molasses 0.5%-2%.
Preferably, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The present invention also provides a kind of preparation method of special animal nutrient for milk cows, comprising:
Extract fulvic acid;
Prepare bitter bean pulp fermentation culture;
Be that the described bitter bean pulp fermentation culture that the described fulvic acid of 40%-60%, Total Flavonoids from Taraxacum mongolicum that percentage by weight is 0.2%-0.5% and percentage by weight are 39.5%-59.5% mixes with percentage by weight.
On the basis of technique scheme, described extraction fulvic acid specifically comprises:
Fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower the extraction 2-3 hour;
The slagging-off of said extracted liquid is rear by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, in temperature 70 C-80 ℃ lower activation 1.5-2 hour, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
It is brown coal, weathered coal or peat that described fulvic acid extracts raw material.
Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature.
Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The invention provides a kind of special animal nutrient for milk cows and preparation method thereof, have the following advantages:
1, improves functions of intestines and stomach, enhancing immunity of organisms.After fulvic acid enters alimentary canal in the special animal nutrient for milk cows of the present invention, can stimulate the coat of the stomach mucous membrane to increase gastric secretion, increase appetite; Because fulvic acid has surface-active, can promote nutrients to enter sooner body again, improve absorptivity and conversion ratio; Can also stimulate beneficial microorganism growth in the milk cow stomach and intestine, suppress the breeding of spoilage organisms, prevent the poisoning of milk cow hydrochloric acid in gastric juice; Fulvic acid has stronger absorption and complexing power, can effectively adsorb poisonous and harmful substance and bacteriotoxin in the stomach, has detoxifcation, alleviates excreta stink and significantly astringing to arrest diarrhea effect, is conducive to the improvement of stable breeding drylot feeding environment.Simultaneously, fulvic acid and Total Flavonoids from Taraxacum mongolicum can promote the activity of multiple probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) propagation and enhancing plurality of enzymes (polyphenol oxidase, peroxidase, ascorbic acid oxidase etc.) in the special animal nutrient for milk cows of the present invention, strengthen body immunocompetent cell and body immune system rejection ability, body immunity is improved.
2, reduce the mammitis incidence of disease, the raising output of milk.Under the effect of various enzymes, the active group in the special animal nutrient for milk cows of the present invention in the fulvic acid molecule can decomposite nascent oxygen, thereby has bactericidal action; Simultaneously, fulvic acid has activation to adrenal cortex, can promote the secretion of adrenaline and cortex hormone of aadrenaline, therefore has the effect of anti-inflammatory and pain relieving.The effect that Total Flavonoids from Taraxacum mongolicum also has antibiotic, anti-inflammatory and relieves inflammation or internal heat as the extract of Chinese medicine dandelion.In view of fulvic acid and Total Flavonoids from Taraxacum mongolicum all have the effect of bactericidal antiphlogistic, special animal nutrient for milk cows of the present invention all has good prevention effect to all kinds of mammitises of milk cow and recessive mastitis etc., and the output of milk of milk cow is significantly improved.
3, safe ready.Special animal nutrient for milk cows of the present invention only need be admixed in proportion to feed in the feed and get final product, and is simple and convenient; Simultaneously, special animal nutrient for milk cows of the present invention does not contain antibiotic and/or antibiotics material, and the raiser can relievedly use milk cow, can not remain in the milk, also can slowly not accumulate in human body by livestock products and environment, the mankind are caused healthy hidden danger.
4, stable performance, easily preservation.The shortcoming of probiotic in the special animal nutrient for milk cows of the present invention (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) is poor heat stability, is in modified, granulation, the heat treatment such as expanded or long-term storage all easily causes inactivation under normal temperature condition; And the active ingredients such as fulvic acid, Total Flavonoids from Taraxacum mongolicum have also been added simultaneously in the special animal nutrient for milk cows of the present invention, become the composite parts product, this compound product can remedy each composition deficiency to each other in the process of processing, preservation, use, make the effect maximization of special animal nutrient for milk cows of the present invention.
Below by embodiment, technical scheme of the present invention is described in further detail.
Description of drawings
Fig. 1 is preparation method's flow chart of special animal nutrient for milk cows of the present invention;
Fig. 2 is the flow chart that the present invention extracts fulvic acid.
The specific embodiment
Further specify the technical scheme of special animal nutrient for milk cows of the present invention below by specific embodiment.
Special animal nutrient for milk cows the first embodiment of the present invention
Special animal nutrient for milk cows comprises the constituent of following percentage by weight: fulvic acid 40%, Total Flavonoids from Taraxacum mongolicum 0.5% and bitter bean pulp fermentation culture 59.5%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and the number of viable of S. cervisiae is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Special animal nutrient for milk cows the second embodiment of the present invention
Special animal nutrient for milk cows comprises the constituent of following percentage by weight: fulvic acid 50%, Total Flavonoids from Taraxacum mongolicum 0.3% and bitter bean pulp fermentation culture 49.7%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Special animal nutrient for milk cows the 3rd embodiment of the present invention
Special animal nutrient for milk cows comprises the constituent of following percentage by weight: fulvic acid 60%, Total Flavonoids from Taraxacum mongolicum 0.2% and bitter bean pulp fermentation culture 39.8%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Special animal nutrient for milk cows the 4th embodiment of the present invention
Special animal nutrient for milk cows comprises the constituent of following percentage by weight: fulvic acid 60%, Total Flavonoids from Taraxacum mongolicum 0.5% and bitter bean pulp fermentation culture 39.5%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
In the technical scheme of the invention described above special animal nutrient for milk cows the first embodiment to the four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Special animal nutrient for milk cows of the present invention can admix according to the ratio of 3-5 kg/ton that feeding cow gets final product in the feed.
Technical scheme by the invention described above special animal nutrient for milk cows the first embodiment to the four embodiment can be found out, compared with prior art, special animal nutrient for milk cows of the present invention has the remarkable result that improves functions of intestines and stomach, enhancing immunity of organisms, the reduction mammitis incidence of disease, the raising output of milk, safe ready, stable performance, easy preservation.
Fig. 1 is preparation method's flow chart of special animal nutrient for milk cows of the present invention, is specially:
Fig. 2 is the flow chart that the present invention extracts fulvic acid, and in technical scheme shown in Figure 1, step 1 is specially:
Step 11, fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it was in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower extraction 2-3 hour;
The part by weight adding mass concentration of step 12, the slagging-off of said extracted liquid is rear by 100: 1-2 is 30% hydrogen peroxide, in temperature 70 C-80 ℃ lower activation 1.5-2 hour, obtains activating solution;
Step 13, with molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
On the basis of technique scheme, it is brown coal, weathered coal or peat that described fulvic acid extracts raw material.Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature.Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
Preparation method first embodiment of special animal nutrient for milk cows of the present invention
Step 101, brown coal powder is broken to 70 orders, it is that extraction is 2.5 hours under temperature 70 C, obtains extract in 4% the sodium hydroxide solution that the brown coal after pulverizing are added mass concentration by 1: 8 part by weight;
Step 102, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1.5 part by weight after the centrifugation slagging-off, activate 2 hours in 75 ℃ of temperature are lower, obtain activating solution;
Step 103, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 50, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.5;
Step 104, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 5 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 60 hours in 20 ℃ of temperature, aerobic fermentation is 16 hours again, obtain bitter bean pulp fermentation culture, number of viable is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 1 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 105, be that 40% described feed grade fulvic acid, percentage by weight are that 0.5% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 59.5% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method second embodiment of special animal nutrient for milk cows of the present invention
Step 201, weathered coal is crushed to 60 orders, it is that extraction is 2 hours under temperature 60 C, obtains extract in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight;
Step 202, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1 part by weight after the centrifugation slagging-off, under temperature 70 C, activate 1.5 hours, obtain activating solution;
Step 203, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 204, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours in 25 ℃ of temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 205, be that 50% described feed grade fulvic acid, percentage by weight are that 0.3% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 49.7% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 3rd embodiment of special animal nutrient for milk cows of the present invention
Step 301, peat dust is broken to 80 orders, it is in 2% the sodium hydroxide solution that the peat after pulverizing is added mass concentration by 1: 9 part by weight, in 80 ℃ of lower extractions of temperature 3 hours, obtains extract;
Step 302, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 2 part by weight after the centrifugation slagging-off, activate 1.8 hours in 80 ℃ of temperature are lower, obtain activating solution;
Step 303, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 80, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=7;
Step 304, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 10 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 38 hours in 40 ℃ of temperature, aerobic fermentation is 36 hours again, obtain bitter bean pulp fermentation culture, number of viable is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 10 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 305, be that 60% described feed grade fulvic acid, percentage by weight are that 0.2% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 39.8% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 4th embodiment of special animal nutrient for milk cows of the present invention
Step 401, weathered coal is crushed to 60 orders, it is that extraction is 2 hours under temperature 60 C, obtains extract in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight;
Step 402, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1 part by weight after the centrifugation slagging-off, under temperature 70 C, activate 1.5 hours, obtain activating solution;
Step 403, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 404, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours in 25 ℃ of temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 405, be that 60% described feed grade fulvic acid, percentage by weight are that 0.5% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 39.5% described bitter bean pulp fermentation culture mixes with percentage by weight.
In the technical scheme of preparation method first embodiment to the of the invention described above special animal nutrient for milk cows four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Can be admixed according to the ratio of 3-5 kg/ton by the special animal nutrient for milk cows of the preparation method of the invention described above special animal nutrient for milk cows preparation that feeding cow gets final product in the feed.
Technical scheme by preparation method the first embodiment to the four embodiment of the invention described above special animal nutrient for milk cows can be found out, compared with prior art, by the special animal nutrient for milk cows of the preparation method of special animal nutrient for milk cows of the present invention preparation have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the mammitis incidence of disease, improve the output of milk, safe ready, stable performance, the remarkable result easily preserved.
The remarkable efficacy of special animal nutrient for milk cows of the present invention is described below by concrete test.
The 40 close cow heads of production status such as body weight, parity, lactation fate, the output of milk, butterfat percnetage are selected in test, be divided into two processed group, every processed group 20 cow heads, with reference to raising dairy cattle standard (China, 2004) preparation basal diet, take the examination ox of the basal diet of feeding as control group, special animal nutrient for milk cows of the present invention is added into according to the ratio of 5 kg/ton consists of the test daily ration in the basal diet, take the examination ox of feeding experiment daily ration as test group.Experimental period is 44 days, and wherein, the phase of raising is 7 days in advance, and the formal test phase is 37 days.
Result of the test shows:
1, milk production of cow significantly improves.Within the whole formal test phase, the average output of milk of control group is by the 30.55kg/ head. day, be increased to the 31.20kg/ head. day, increased the 0.65kg/ head. day; The average output of milk of test group is by the 30.16kg/ head. day, be increased to the 32.21kg/ head. day, increase the 2.05kg/ head. day, test group has increased the 1.4kg/ head than the average output of milk of control group. day.
2, milk quality significantly improves.Within the whole formal test phase, the thin spore of the body in the test group milk drops to 370,000/ML by 1,012 ten thousand/ML, the 9750000/ML that descended, and the essentially no variation of control group.
In addition, lot of experimental data proves, behind the edible feed that adds special animal nutrient for milk cows of the present invention, can prolong 36-39 days lactation period to milk cow for a long time, and the incidence of disease of all kinds of mammitises and recessive mastitis significantly reduces.
Special animal nutrient for milk cows of the present invention has the remarkable result that improves functions of intestines and stomach, enhancing immunity of organisms, the reduction mammitis incidence of disease, the raising output of milk, safe ready, stable performance, easy preservation.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the spirit and scope of technical solution of the present invention.
Claims (2)
1. special animal nutrient for milk cows is characterized in that, comprises the constituent of following percentage by weight: fulvic acid 40%-60%, Total Flavonoids from Taraxacum mongolicum 0.2%-0.5% and bitter bean pulp fermentation culture 39.5%-59.5%; Described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, composite microbial inoculant liquid; Described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%; Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu; The bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature.
2. the preparation method of special animal nutrient for milk cows according to claim 1 is characterized in that:
It is brown coal, weathered coal or peat that described fulvic acid extracts raw material;
Described extraction fulvic acid specifically comprises:
Fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower the extraction 2-3 hour;
The slagging-off of said extracted liquid is rear by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, in temperature 70
℃Activation is 1.5-2 hour under-80 ℃, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed hymatomalenic acid, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid;
Prepare bitter bean pulp fermentation culture;
Be that the described bitter bean pulp fermentation culture that the described fulvic acid of 40%-60%, Total Flavonoids from Taraxacum mongolicum that percentage by weight is 0.2%-0.5% and percentage by weight are 39.5%-59.5% mixes with percentage by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105924830A CN101999554B (en) | 2010-12-08 | 2010-12-08 | Special animal nutrient for milk cows and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105924830A CN101999554B (en) | 2010-12-08 | 2010-12-08 | Special animal nutrient for milk cows and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101999554A CN101999554A (en) | 2011-04-06 |
| CN101999554B true CN101999554B (en) | 2013-04-17 |
Family
ID=43807443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105924830A Expired - Fee Related CN101999554B (en) | 2010-12-08 | 2010-12-08 | Special animal nutrient for milk cows and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101999554B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669486B (en) * | 2012-06-11 | 2014-02-26 | 内蒙古永业农丰生物科技有限责任公司 | Special animal biogen for shrimps and preparation method thereof |
| CN102669487B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Animal biogen special for pigs and preparation method thereof |
| CN102669436B (en) * | 2012-06-11 | 2013-12-11 | 内蒙古永业农丰生物科技有限责任公司 | Special animal life element for fish and preparation method for same |
| CN102669485B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Animal biotin special for broilers and preparing method thereof |
| CN102669484B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Special animal biogen for laying hens and preparation method of special animal biogen |
| CN105614085B (en) * | 2016-01-12 | 2020-12-08 | 内蒙古农业大学 | Preparation method of high-activity cow fermented concentrated feed and special composite microbial agent thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843157A (en) * | 2006-05-15 | 2006-10-11 | 河北农业大学 | Chinese herbal medicine feed additive for dairy cows |
| CN1935182A (en) * | 2005-09-20 | 2007-03-28 | 张伟巍 | Chinese herbal medicine compound immunopotentiator for animal |
| CN1951208A (en) * | 2005-10-21 | 2007-04-25 | 内蒙古永业生物技术有限责任公司 | Nutrient for animal and preparation process thereof |
-
2010
- 2010-12-08 CN CN2010105924830A patent/CN101999554B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935182A (en) * | 2005-09-20 | 2007-03-28 | 张伟巍 | Chinese herbal medicine compound immunopotentiator for animal |
| CN1951208A (en) * | 2005-10-21 | 2007-04-25 | 内蒙古永业生物技术有限责任公司 | Nutrient for animal and preparation process thereof |
| CN1843157A (en) * | 2006-05-15 | 2006-10-11 | 河北农业大学 | Chinese herbal medicine feed additive for dairy cows |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101999554A (en) | 2011-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108813098B (en) | Microbial fermentation composite antibiotic-free feed containing natural antibiotic components | |
| CN1969657B (en) | Probiotic feed additive used for pig | |
| CN103053802B (en) | Chinese herb fermented feed for improving laying rate of laying fowl and production method of feed | |
| CN101999554B (en) | Special animal nutrient for milk cows and preparation method thereof | |
| WO2013183805A1 (en) | Additive composition for shrimp feed and a method for producing the same | |
| CN102366023A (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN102379374B (en) | High-activity microbial protein forage and preparation method thereof | |
| CN1853497A (en) | Improvement of cow milk output and milk quality by Chinese medicinal herb composite microbial forage additive | |
| CN101999555B (en) | Animal nutrient special for sheep and method for preparing the same | |
| CN103141702B (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN109511810A (en) | A kind of preparation method and applications of the microbiological feed for milking sow | |
| CN103798541A (en) | Antibiotic-free pig feed used in growing stage | |
| CN109007370A (en) | Complex function additive for feed for piglets and preparation method thereof | |
| CN106509440A (en) | Health-care feed additive for multiparous sows | |
| CN101999556B (en) | Special animal nutrient for beef cattle and preparation method thereof | |
| CN110521785A (en) | Probiotics fermention functional food and its preparation | |
| CN104206789A (en) | Feed additive for preventing cow mastitis and brucellosis and preparation method thereof | |
| CN112352874A (en) | Anti-piglet diarrhea probiotic preparation and preparation method thereof | |
| CN102367416A (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN107594084A (en) | A kind of non-resistant ecological feed formula of growing and fattening pigs | |
| CN104982665A (en) | Preparation method of probiotic fermentation cattle feed additive | |
| CN110973397A (en) | Compound Chinese herbal medicine micro-ecological antibiotic-free feed additive for laying hens and preparation method | |
| CN102669486B (en) | Special animal biogen for shrimps and preparation method thereof | |
| CN102669485B (en) | Animal biotin special for broilers and preparing method thereof | |
| CN109221697A (en) | Weaned piglets probiotic feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: INNER MONGOLIA SLOAN BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: INNER MONGOLIA YONGYE BIOTECHNOLOGY CO., LTD. Effective date: 20140519 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 010070 HOHHOT, INNER MONGOLIA AUTONOMOUS REGION TO: 011517 HOHHOT, INNER MONGOLIA AUTONOMOUS REGION |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20140519 Address after: 011517 the Inner Mongolia Autonomous Region Shengle Economic Zone on the south side of four street Hohhot city Shengle (munte earth company) Patentee after: INNER MONGOLIA SILONG BIOTECHNOLOGY CO., LTD. Address before: 010070 the Inner Mongolia Autonomous Region Jinshan Jinshan Avenue Hohhot economic and Technological Development Zone Yongye Industrial Park Patentee before: Inner Mongolia Yongye Nongfeng Biotechnology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130417 Termination date: 20201208 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |