CN101990825B - Method for preparing mycorrhiza industrial products - Google Patents
Method for preparing mycorrhiza industrial products Download PDFInfo
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- CN101990825B CN101990825B CN2010102639882A CN201010263988A CN101990825B CN 101990825 B CN101990825 B CN 101990825B CN 2010102639882 A CN2010102639882 A CN 2010102639882A CN 201010263988 A CN201010263988 A CN 201010263988A CN 101990825 B CN101990825 B CN 101990825B
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- clay
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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Abstract
The invention discloses a method for preparing mycorrhiza industrial products, and belongs to the technical field of mycorrhizae production method. The production process includes: treating symbiotic clay at the room temperature so as to enable the symbiotic clay be suitable for fungus culture, inoculating arbuscular mycorrhizal fungi to be propagated to the symbiotic clay, and putting into a culture medium for culturing for some time; then mixing contagious clay serving as an initial point with main plant seeds on the ground, through fertilizing and watering, propagating a large number of mushrooms; regulating the mushrooms to form spores, and separating by a wet screening method to prepare the industrial products. The preparation method can be widely used without limitation. The products are solid produces and are suitable for mass production with high enrichment degree, high accumulation speed of strain criminal body quantity, medium infection rate and pure product. When the preparation method of the invention is applied to tree planting and forestation, the survival rate of transplanting of nursery stocks can be improved, and vegetation recovery can be promoted, meanwhile the preparation method is applied to reafforestation, forestry reconstruction, land improvement, the planting of problem sites and reviviscence of valuable frees.
Description
Technical field
The present invention relates to the preparation method of the mycorhiza of a kind of root system of plant and mycosymbiosis formation, particularly a kind of preparation method of mycorhiza industrial goods.
Background technology
A kind of symbiosis association that mycorhiza is that root system of plant and fungi form in the soil.According to nearly 90% higher plant on the statistics earth and mycorrhizal fungi (being called for short the mycorhiza bacterium) formation symbiotic relation.In this symbiotic relation, plant provides carbohydrate for the mycorhiza bacterium, and the mycorhiza bacterium is then improved plant to mineral nutrient absorbing in the soil through its huge mycelia network.It is whole to set up favourable, conditional each other physiology between mycorhiza bacterium and the plant, and morphological feature is respectively arranged, and this is the typical case's representative that realizes symbiotic relation between the eucaryote.The effect of mycorhiza mainly is to enlarge the root system absorbing surface, increases the absorbing capacity to the outer element (particularly phosphorus) of primitive root hair absorption region.Mycorhiza bacterium mycelium both to all soil expansions of root, communicated with the host plant tissue again, from host plant, absorbed the nutrition of organic substance conducts oneself such as carbohydrate on the one hand, from soil, absorbed nutrient, moisture supply plant on the other hand again.
Arbuscular mycorrhiza (Arbuscular mycorrhizae AM) is called for short the AM fungi, and it is one type of fungi of most widespread in the soil symbiosis fungi, at least can with 200,000 plants row commensalisms more than the kind of 200 sections.The mycorhiza that the big pluripotent human worker of prior art turns out the AM fungi, but its spore is processed the technology of industrial goods, not report in the document.If process industrial goods, it is convenient then to preserve, and raising the output also improves quality.
Existingly can roughly be summarised as about the document of mycorhiza and the report of patent: the application targetedly of corresponding product is cultivated and turned out to the mycorhiza of (1) a certain fungi; Like application number is 02159722.7, and name is called " method of producing the AMF microbial inoculum "; (2) artificial culture goes out the mycorhiza of a kind of fungi, be applied in a certain specific field, and be 200310123406.0 like application number, name is called " use the mycorhiza technology and recover the method for metal tailings vegetation "; (3) more relevant of how to cultivate artificial mycorhiza are improved one's methods, like the improvement of the disclosed relevant medium of a lot of patent documentations, the improvement of method for distilling, improvement of detection method or the like.
In disclosed document, also there is corresponding defects, strong excessively like (1) specific aim, just disclose the method that a kind of fungi is processed mycorhiza, and this mycorhiza is applied in a kind of application of specific technical field, compatibility is not high, and the product effect is more single; (2) it is bigger the mycorhiza of having turned out on the market to be applied to the limitation of frontier; (3) a lot of mycorhiza products all are to produce fluid product, because fluid product is contaminated easily, are not suitable for a large amount of commercial production.
According to above-mentioned bibliographical information, if can produce the solid-state industrial goods of the stronger mycorhiza of a kind of compatibility, and be applied in the various aspects in a certain field, then be fit to a large amount of production, application and marketing type are extremely strong.
Summary of the invention
For solving the problems of the technologies described above, the present invention provides a kind of preparation method of mycorhiza industrial goods, and the technical problem of solution is that mycorhiza is processed solid-state industrial goods, can pack use, and appearance color is pure, no living contaminants; And solved its application problem in afforestation, comprised that reforestation, forest land reconstruction, soil are used instead, problem location is planted and valuable trees demutation.
The present invention realizes through following technical scheme:
A kind of preparation method of mycorhiza industrial goods is to realize through following step:
(1) be 15-22 ℃ in temperature, relative moisture is to carry out the expansion of clay under the condition of 60%-70%, makes the clay size reach 2-4mm, and puts into medium, and room temperature is laid;
AM fungi strain to be bred is inoculated in the medium cultivates;
Infectious clay as rise sending out a point, being mixed with the AM fungi after the said cultivation, being seeded in undergroundly again with main plant seed, make the breeding of bacterium mushroom;
(2) growing plants is applied fertilizer and waters; Make plant roots penetrate the clay after enlarging, also be diffused into the bacterium mushroom plant root of whole growth, reach the best breeding of bacterium mushroom after; Through changing cultural method plant is die, the bacterium mushroom forms fungal spore in the col of clay;
(3) adopt wet screening from clay, to isolate fungal spore, the clay after the separation saves as the part of bacterium mushroom, and fungal spore is processed graininess, and packing is processed solid-state industrial goods.
Said medium is peat sandy soil medium, and the mass ratio of said medium and said clay is 1: 1.
Said infectious clay be acid-base value to be controlled at the pH value be 5-7, the clay that forms after moist heat sterilization 1-2 hour.
The best breeding of said bacterium mushroom is through controlling the adjusting of fertilizer, control water and weeding and pest-killing, after sowing 3-4 is individual month, and a kind of state of bacterium mushroom that reaches.
It is said that plant is die is to realize through the number of times that adjusting is watered through changing cultural method.
The fungal spore pH value that said wet screening screens is controlled at 5-7.
Clay after the said separation contains tiny tree root, saves as the part of bacterium mushroom, and the clay after the said separation is dried under 15% moisture content.
Beneficial effect of the present invention is:
Can be widely used, no limitation, and be solid-state industrial goods, being fit to a large amount of production, enrichment is high, and bacterial classification crime body quantity accumulative total speed is high, and infection rate is medium, and product is pure; And the present invention can be utilized in the afforestation, and reforestation, forest land reconstruction, soil are used instead, problem location is planted and valuable trees demutation can be used.
Embodiment
Below in conjunction with embodiment, preparation of the present invention and the application in afforestation are further specified.
Embodiment 1
(1) be 15 ℃ in temperature, relative moisture is to carry out the expansion of clay under 60% the condition, makes the clay size reach 2mm, and puts into peat sandy soil medium, and regulating and making the mass ratio of peat sandy soil medium and said clay is 1: 1, and room temperature is laid; AM fungi strain to be bred is inoculated in the peat sandy soil medium cultivates, transferring the acid-base value of clay to be controlled at the pH value is 5, and moist heat sterilization 1 hour forms infectious clay;
Infectious clay as rise sending out a point, being mixed with the AM fungi after the said cultivation, being seeded in undergroundly again with main plant seed, make the breeding of bacterium mushroom;
(2) growing plants is applied fertilizer and waters; Make plant roots penetrate the clay after enlarging, also be diffused into the bacterium mushroom plant root of whole growth, through controlling the adjusting of fertilizer, control water and weeding and pest-killing; After sowing 3 months; After reaching the best breeding of bacterium mushroom, thereby change cultural method through the number of times that adjusting is watered plant is die, the bacterium mushroom forms fungal spore in the col of clay;
(3) adopt wet screening from clay, to isolate fungal spore, the clay after the separation saves as the part of bacterium mushroom, and fungal spore is processed graininess, and packing is processed solid-state industrial goods.Wherein the fungal spore pH value that screens of wet screening is controlled at 5; Clay after the separation contains tiny tree root, saves as the part of bacterium mushroom, and the clay after the said separation is dried under 15% moisture content.
Embodiment 2
(1) be 22 ℃ in temperature, relative moisture is to carry out the expansion of clay under 70% the condition, makes the clay size reach 4mm, and puts into peat sandy soil medium, and regulating and making the mass ratio of peat sandy soil medium and said clay is 1: 1, and room temperature is laid; AM fungi strain to be bred is inoculated in the peat sandy soil medium cultivates, transferring the acid-base value of clay to be controlled at the pH value is 7, and moist heat sterilization 2 hours forms infectious clay;
Infectious clay as rise sending out a point, being mixed with the AM fungi after the said cultivation, being seeded in undergroundly again with main plant seed, make the breeding of bacterium mushroom;
(2) growing plants is applied fertilizer and waters; Make plant roots penetrate the clay after enlarging, also be diffused into the bacterium mushroom plant root of whole growth, through controlling the adjusting of fertilizer, control water and weeding and pest-killing; After sowing 4 months; After reaching the best breeding of bacterium mushroom, thereby change cultural method through the number of times that adjusting is watered plant is die, the bacterium mushroom forms fungal spore in the col of clay;
(3) adopt wet screening from clay, to isolate fungal spore, the clay after the separation saves as the part of bacterium mushroom, and fungal spore is processed graininess, and packing is processed solid-state industrial goods.Wherein the fungal spore pH value that screens of wet screening is controlled at 7; Clay after the separation contains tiny tree root, saves as the part of bacterium mushroom, and the clay after the said separation is dried under 15% moisture content.
Embodiment 3
(1) be 19 ℃ in temperature, relative moisture is to carry out the expansion of clay under 65% the condition, makes the clay size reach 3mm, and puts into peat sandy soil medium, and regulating and making the mass ratio of peat sandy soil medium and said clay is 1: 1, and room temperature is laid; AM fungi strain to be bred is inoculated in the peat sandy soil medium cultivates, transferring the acid-base value of clay to be controlled at the pH value is 6, and moist heat sterilization 2 hours forms infectious clay;
Infectious clay as rise sending out a point, being mixed with the AM fungi after the said cultivation, being seeded in undergroundly again with main plant seed, make the breeding of bacterium mushroom;
(2) growing plants is applied fertilizer and waters; Make plant roots penetrate the clay after enlarging, also be diffused into the bacterium mushroom plant root of whole growth, through controlling the adjusting of fertilizer, control water and weeding and pest-killing; After sowing 4 months; After reaching the best breeding of bacterium mushroom, thereby change cultural method through the number of times that adjusting is watered plant is die, the bacterium mushroom forms fungal spore in the col of clay;
(3) adopt wet screening from clay, to isolate fungal spore, the clay after the separation saves as the part of bacterium mushroom, and fungal spore is processed graininess, and packing is processed solid-state industrial goods.Wherein the fungal spore pH value that screens of wet screening is controlled at 6; Clay after the separation contains tiny tree root, saves as the part of bacterium mushroom, and the clay after the said separation is dried under 15% moisture content.
More than the product of 3 embodiment through quality control, spore quantity, TU transmission unit quantity and the effect on plant all meet standard-required.
Below be the application of the present invention in afforestation.
Embodiment 4
Forest land reconstruction under the pine tree
Forest land reconstruction with the unbalanced softwood forest single structure that distributes be converted near natural, be full of vitality, have the transition stage in management advantage forest land.
Certain forest land gross area is 108.8 ten thousand hectares; The soil texture is Z2 (sandy); Fraction plantation Chinese parasol tree, summer oak, durmast and Carpinus betulus, wherein 86.13 (77.8%) is the pine tree woods, the pine tree woods of existence only accounts for 13% naturally; Seeds distribute and depart from nature on a large scale, need carry out the forest land reconstruction and make it be in more natural, healthy state.
1 year, planted inhomogeneous broad leaved forest a sandy softwood forest test, like maple, Oak Tree and fagus, the industrial goods that the present invention produces are all used in all plantations.
Observed the growth progress on the 3rd year.The durmast root has the mycorhiza structure, and summer oak root has the mycorhiza structure, observes living away from home of endotrophic mycorrhiza fungi structure (mycelia, clump branch and spore) and ectotrophic mycorrhiza at the Oak Tree root.Maple is also taken a sample; It is well parasitic to observe vesicle-AMF; Some tip of a root can see something of the rhizomorph of ectotrophic mycorrhiza fungi, because do not observe the ectotrophic mycorrhiza typical case root tip of a root, rhizomorph also possibly come from adjacent pine tree.
The 4th year, there are not dead trees, better elongation growth.
Conclusion: in the plantation of broad leaved forest, use mycorhiza and impel good growth.The mycorhiza symbiosis has improved the resistance for arid.Particularly very arid summer.
Embodiment 5
The soil is used instead
There is concrete regulation various countries to the heavier mask of crops, thereby exist in former arable land not in situation about using as agricultural.At first to consider this soil whether exist corrosion dangerous be in unfavorable conditions (sandy, arid, nutrition is barren).It is that the fallow land reconstruction is caused forest land or energy crop plantation that another one is selected.The soil lacks trees, the needed symbiosis fungi of especially special trees through the agricultural use of decades.This possibly cause bad plant growing, the slow growth at high mortality and initial stage.
On an arable land, plant Oak Tree, trees are used the combined treatment of ectotrophic mycorrhiza fungi, Nei Sheng army mycorrhiza fungi or two types respectively.
Observe the especially strong and better growth of the trees performance that ectotrophic mycorrhiza or combined inoculation are handled after 2 years.What the trees of only handling with endotrophic mycorrhiza grew in 1 year is not fine.Proof ectotrophic mycorrhiza fungi is that the Oak Tree growth is necessary, the ectotrophic mycorrhiza fungi is applied to full wafer ploughs, and observes well-grown once more.
Except that the foregoing description, the present invention also can dare to reforesting.Problem location plantation and valuable trees demutation.
Increased the space effective property of nutrient elements such as Soil Nitrogen, phosphorus, potassium.The existence of mycorhiza is the important symbol of healthy ecology system.Mycorhiza can promote plant absorbing to utilize mineral nutrient and moisture, improves crop anti-adversity and disease resistance, and the structure of improving the soil strengthens soil fertility, improves the nursery stock transplanting survival rate, promotes revegetation.
Claims (4)
1. the preparation method of mycorhiza industrial goods is characterized in that being realizing through following step:
(1) be 15-22 ℃ in temperature, relative moisture is to carry out the expansion of clay under the condition of 60%-70%, makes the clay size reach 2-4mm, and puts into medium, and room temperature is laid;
AM fungi strain to be bred is inoculated in the medium cultivates, transferring the acid-base value of clay to be controlled at the pH value is 5-7, after moist heat sterilization 1-2 hour, forms infectious clay;
Infectious clay as rise sending out a point, being mixed with the AM fungi after the said cultivation, being seeded in undergroundly again with main plant seed, make the breeding of bacterium mushroom;
(2) growing plants is applied fertilizer and waters; Make plant roots penetrate the clay after enlarging, also be diffused into the bacterium mushroom plant root of whole growth, through controlling the adjusting of fertilizer, control water and weeding and pest-killing; After sowing 3-4 is individual month; After reaching the best breeding of bacterium mushroom, thereby change cultural method through the number of times that adjusting is watered plant is die, the bacterium mushroom forms fungal spore in the col of clay;
(3) adopt wet screening from clay, to isolate fungal spore, the clay after the separation saves as the part of bacterium mushroom, and fungal spore is processed graininess, and packing is processed solid-state industrial goods.
2. the preparation method of mycorhiza industrial goods as claimed in claim 1 is characterized in that said medium is peat sandy soil medium, and the mass ratio of said medium and said clay is 1: 1.
3. the preparation method of mycorhiza industrial goods as claimed in claim 1 is characterized in that the fungal spore pH value that said wet screening screens is controlled at 5-7.
4. the preparation method of mycorhiza industrial goods as claimed in claim 1 is characterized in that the clay after the said separation contains tiny tree root, saves as the part of bacterium mushroom, and the clay after the said separation is dried under 15% moisture content.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010102639882A CN101990825B (en) | 2010-08-24 | 2010-08-24 | Method for preparing mycorrhiza industrial products |
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| CN2010102639882A CN101990825B (en) | 2010-08-24 | 2010-08-24 | Method for preparing mycorrhiza industrial products |
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| CN101990825B true CN101990825B (en) | 2012-04-18 |
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| CN110063160A (en) * | 2018-01-20 | 2019-07-30 | 杭州绿风生态环境建设集团有限公司 | A kind of arbuscular mycorrhizal fungi screening technique conducive to garden plant growth |
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| CN1255528C (en) * | 2002-12-30 | 2006-05-10 | 中国农业大学 | Method for producing bush mycorrhizal fungi preparation |
| CN101341818B (en) * | 2008-08-26 | 2011-11-23 | 新疆农业科学院微生物应用研究所 | Arbuscular mycorrhizal fungi dry powder seed-dressing agent and preparation method thereof |
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