CN101978888B - Method for preparing microalgae polypeptide powder and microalgae polypeptide powder-containing flavoring - Google Patents
Method for preparing microalgae polypeptide powder and microalgae polypeptide powder-containing flavoring Download PDFInfo
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Abstract
The present invention relates to a microalgae polypeptide powder-containing flavoring with rich nutrition, and a preparation method thereof. The method comprises the following steps of: crushing microalgae meal, adding the microalgae meal into water and mixing; adding protease and performing enzymolysis for 4 to 7 hours; and deactivating enzyme, concentrating enzymolysis solution, adding an embedding medium, embedding and finally performing spray-drying to obtain the microalgae polypeptide powder. Because the microalgae meal is used as the raw material, and high molecular protein in the microalgae meal is enzymolyzed into polypeptide and amino acid which can be easily absorbed by human bodies by using biological engineering enzymolysis technology, the produced product has high content of protein and amino acid, rich nutrition and high freshness and contains docosahexaenoic acid (DHA) microalgae oil; the product can be used for an flavoring type essence reaction essence group and used for the industries such as biological pharmacy, health-care products, household flavorings, instant noodle flavorings, puffed foods, baked foods, seafood processed products and the like, and is a good method for improving the added value of the microalgae meal.
Description
Technical field
The present invention relates to a kind of little algae deep process technology, particularly a kind of method that contains the flavouring of little algae polypeptide powder and the algae dregs of rice are prepared into little algae polypeptide powder.
Background technology
Little algae is a kind of nutritious and balanced, and is of value to the algae of health, not only is rich in DHA, also is rich in the multiple nutritional components such as protein, amino acid, polysaccharide.
The algae dregs of rice are the residues after little algae is refined grease, and its main component is protein, amino acid, and contain a certain amount of DHA algae oil, have higher nutritive value and medical value.Existing little algae deep processing mainly concentrates on the preparation of the little algae oil of DHA, and the algae dregs of rice after the extraction oil are usually processed as feed, and the composition that wherein enriches particularly protein is not well utilized, and is a kind of invisible wasting of resources.
Summary of the invention
The object of the invention is for above-mentioned existing problems and deficiency, provide a kind of utilize the algae dregs of rice for raw material prepare the Amino acid and protein content height, nutritious, freshness is high and contain the method for little algae polypeptide powder of DHA algae oil.
The invention still further relates to a kind of utilize that little algae polypeptide powder forms for core material is composite have nutritious, freshness is high and contain the nutritional flavouring agent of DHA algae wet goods characteristics.
Technical scheme of the present invention is achieved in that
The flavouring that contains little algae polypeptide powder of the present invention is comprised of the component of following weight proportion:
Little algae polypeptide powder 10-20 part, basic flavoring 28-52 part, tasty agents 15-26 part, be flavor agent 6-10 part, spice 2-4 part, filler 0-6.5 part, anticaking agent 0-2 part, seafood essence 0-0.2 part.
Wherein above-mentioned little algae polypeptide powder is core nutritious flavouring raw material, above-mentioned basic flavoring comprises: the sucrose of the salt of 20-40 part, 8-12 part, the glucose of 5-8 part, above-mentioned tasty agents comprises the monosodium glutamate of 15-25 part, the I+G of 0.5-1 part, above-mentionedly be the flavor agent and comprise the yeast extract of 3-5 part and the hydrolyzed vegetable protein of 3-5 part, to strengthen voluptuousness.Above-mentioned spice comprises the garlic powder of 1-2 part, the ginger powder of 1-2 part, can remove raw meat, increase flavour.It is fragrant that seafood essence can increase the product head.Above-mentioned filler comprises the cornstarch of 0-2.3 part and the maltodextrin of 0-3.8 part.
Above-mentioned flavouring is owing to be that to be that the core nutrient raw material is composite with little algae polypeptide powder form, thus have nutritious, freshness is high and contain DHA algae wet goods characteristics.
The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds water and mixes;
(2), added protease hydrolyzed 4-7 hour;
(3), with enzyme-deactivating, concentrated enzymolysis liquid then;
(4), add embedding medium, embedding, last spray-drying namely obtain little algae polypeptide powder.
Wherein the method for above-mentioned protease hydrolyzed is to adopt endopeptidase and peptide bond excision enzyme to carry out two enzyme substep enzymolysis, and wherein said endopeptidase is compound protease or papain, and described peptide bond excision enzyme is flavor protease.Form more oligopeptide because the former percent hydrolysis is high, easily make product with bitter taste, the latter forms is mainly amino acid, is mainly delicate flavour, and bitter taste is not obvious.With both take certain proportion (ratio of endopeptidase and excision enzyme is as 1:2-1:3) add and use the substep enzymolysis both can make among the present invention product amino acid content reach higher in, can make again its flavour reach better.
The algae dregs of rice among the present invention be marine microalgae (split the kettle algae (
Schizochytrium sp.), my Ken Shi kettle algae (
Ulkenia amoeboida), the hidden dinoflagellate of Kou Shi (
Crypthecodinium cohnii) etc.) refine the residue behind the grease.
The present invention is owing to adopt take the algae dregs of rice as raw material, and utilize the modern biological project zymolysis technique macromolecular protein digestion to be become to be easy to polypeptide and the amino acid of absorption of human body, compare with acid hydrolysis technology commonly used at present, enzymatic hydrolysis condition is gentle, be easy to control, avoided the generation of carcinogen-chloropropyl alcohol in the acid hydrolysis process, without the discharging of waste water and gas, belonged to environmentally friendly simultaneously.And Amino acid and protein content is high, nutritious in the product of the present invention, freshness is high and contain DHA algae oil, not only can be used for conditioning-type essence reaction perfume base, also can be used for bio-pharmaceuticals, nutrient and healthcare products, Domestic flavouring product, condiment for instant noodles, dilated food, bakery, the industries such as seafood processed goods are a kind of methods of good lifting algae dregs of rice added value.In addition the amino acid that extracts is applied in the flavouring as atural spice base-material or reaction base-material with being gustin, can greatly improves class and the technology content of flavouring, strengthen the flavouring industry market competitiveness at home and abroad.
The specific embodiment
The flavouring that contains little algae polypeptide powder of the present invention is comprised of the component of following weight proportion:
Little algae polypeptide powder 10-20 part, basic flavoring 28-52 part, tasty agents 15-26 part, be flavor agent 6-10 part, spice 2-4 part, filler 0-6.5 part, anticaking agent 0-2 part, seafood essence 0-0.2 part.
Wherein above-mentioned basic flavoring comprises: the sucrose of the salt of 20-40 part, 8-12 part, the glucose of 5-8 part, above-mentioned tasty agents comprises the monosodium glutamate of 15-25 part, the I+G of 0.5-1 part (flavour nucleotide disodium), above-mentioned be the flavor agent comprise the yeast extract of 3-5 part and the hydrolyzed vegetable protein of 3-5 part, above-mentioned spice comprises the garlic powder of 1-2 part, the ginger powder of 1-2 part, and above-mentioned filler comprises the cornstarch of 0-2.3 part and the maltodextrin of 0-3.8 part.
The preparation method of above-mentioned flavouring comprises the steps:
First seafood essence and salt, monosodium glutamate, sucrose, glucose are mixed, and then with little algae polypeptide powder, yeast extract, hydrolyzed vegetable protein HVP, garlic powder, ginger powder, I+G, anticaking agent, cornstarch, the maltodextrin mixing and stirring of above-mentioned weight proportion, namely obtain the flavouring of the present invention of powdery.
Below in conjunction with example, the preparation method of flavouring of the present invention is described in further detail.
Embodiment one:Take by weighing the component of following weight proportion: 20 kilograms of little algae polypeptide powders, 25 kilograms of salt, 12 kilograms of sucrose, 4 kilograms of yeast extracts, 20 kilograms of monosodium glutamates, 8 kilograms of glucose, 3 kilograms of hydrolyzed vegetable protein HVP, 1.5 kilograms in garlic powder, 2 kilograms of ginger powders, 1 kilogram of I+G, 0.2 kilogram in seafood essence, 2.3 kilograms of cornstarch, 2 kilograms of anticaking agents, first seafood essence and salt, monosodium glutamate, sucrose, glucose are mixed, and then with other component mixing and stirring, namely obtain flavouring of the present invention.
Embodiment two:Take by weighing the component of following weight proportion: 20 kilograms of little algae polypeptide powders, 20 kilograms of salt, 12 kilograms of sucrose, 5 kilograms of yeast extracts, 20 kilograms of monosodium glutamates, 8 kilograms of glucose, 5 kilograms of hydrolyzed vegetable protein HVP, 2 kilograms in garlic powder, 1 kilogram of ginger powder, 1 kilogram of I+G, 0.2 kilogram in seafood essence, 1 kilogram of anticaking agent, 3.8 kilograms of maltodextrins, first seafood essence and salt, monosodium glutamate, sucrose, glucose are mixed, and then with other component mixing and stirring, namely obtain flavouring of the present invention.
Embodiment three:Take by weighing the component of following weight proportion: 10 kilograms of little algae polypeptide powders, 40 kilograms of salt, 8 kilograms of sucrose, 5 kilograms of yeast extracts, 15 kilograms of monosodium glutamates, 7 kilograms of glucose, 3 kilograms of hydrolyzed vegetable protein HVP, 2 kilograms in garlic powder, 1 kilogram of ginger powder, 0.5 kilogram of I+G, 0.2 kilogram in seafood essence, 2 kilograms of anticaking agents, 2.3 kilograms of cornstarch, 3.8 kilograms of maltodextrins, first seafood essence and salt, monosodium glutamate, sucrose, glucose are mixed, and then with other component mixing and stirring, namely obtain flavouring of the present invention.
Embodiment four:Take by weighing the component of following weight proportion: 16 kilograms of little algae polypeptide powders, 25 kilograms of salt, 10 kilograms of sucrose, 4 kilograms of yeast extracts, 15 kilograms of monosodium glutamates, 5 kilograms of glucose, 4 kilograms of hydrolyzed vegetable protein HVP, 1.5 kilograms in garlic powder, 1.5 kilograms of ginger powders, 1 kilogram of I+G, first salt, monosodium glutamate, sucrose, glucose are mixed, and then with other component mixing and stirring, namely obtain flavouring of the present invention.
Embodiment five:Take by weighing the component of following weight proportion: 15 kilograms of little algae polypeptide powders, 30 kilograms of salt, 10 kilograms of sucrose, 3 kilograms of yeast extracts, 25 kilograms of monosodium glutamates, 5 kilograms of glucose, 3 kilograms of hydrolyzed vegetable protein HVP, 1 kilogram in garlic powder, 1 kilogram of ginger powder, 0.75 kilogram of I+G, 0.2 kilogram in seafood essence, 2 kilograms of anticaking agents, 2.05 kilograms of cornstarch, 2 kilograms of maltodextrins, first seafood essence and salt, monosodium glutamate, sucrose, glucose are mixed, and then with other component mixing and stirring, namely obtain flavouring of the present invention.
The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds water and mixes;
(2), added protease hydrolyzed 4-7 hour;
(3), with enzyme-deactivating, concentrated enzymolysis liquid then;
(4), add embedding medium, embedding, last spray-drying namely obtain little algae polypeptide powder.
Wherein the addition of above-mentioned water be generally algae dregs of rice powder weight 8-12 doubly.The hydrolysis temperature of above-mentioned protease is controlled at 45-60 ℃.Above-mentioned embedding medium is beta-schardinger dextrin-, converted starch or lactalbumin.
For making enzymatic hydrolysis condition of the present invention gentle, be easy to control, avoid simultaneously the generation of carcinogen-chloropropyl alcohol in the acid hydrolysis process and avoid waste discharge waste gas, the method of above-mentioned protease hydrolyzed is to adopt endopeptidase and peptide bond excision enzyme to carry out two enzyme substep enzymolysis, wherein said endopeptidase is compound protease or papain, and described peptide bond excision enzyme is flavor protease.The consumption of above-mentioned endopeptidase is the 1-2 ‰ of algae dregs of rice powder weight.The consumption of above-mentioned peptide bond excision enzyme is the 3-6 ‰ of algae dregs of rice powder weight.
Below in conjunction with example, the preparation method of little algae polypeptide powder of the present invention is described in further detail.
The content of following each component if no special instructions, all take the weight of algae dregs of rice powder as benchmark, as adds 1 ‰ protease, and then the addition of protease is 1 ‰ of algae dregs of rice powder weight.
Embodiment 1:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are ground into powder, getting algae dregs of rice powder adds 8 times of water gagings and mixes;
(2), under 45 ℃, add first 1 ‰ endopeptidase (compound protease, Novozymes Company) enzymolysis 1h, add again 3 ‰ peptide bond excision enzyme (flavor protease, promise are only believed company) enzymolysis 6h;
(3), be warming up on the basis of the above again 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrated enzymolysis liquid weight), add the converted starch that accounts for concentrated enzymolysis liquid weight 10% at concentrated enzymolysis liquid again, then 90 ℃ of left and right sides embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains little algae polypeptide powder of the present invention.
Embodiment 2:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds 10 times of water gagings and mixes;
(2), under 50 ℃, add first 2 ‰ endopeptidases (papain A1, Guangxi Pang Bo biotechnology) enzymolysis 1h, add again 4 ‰ peptide bond excision enzyme (flavor protease, Guangxi Pang Bo biotechnology) enzymolysis 4h;
(3), be warming up to 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrate weight), add the lactalbumin that accounts for concentrate weight 10% at concentrated enzymolysis liquid again, then in 90 ℃ of lower embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains little algae polypeptide powder of the present invention.
Embodiment 3:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds 12 times of water gagings and mixes;
(2), under 55 ℃, add first 1 ‰ endopeptidases (compound protease, Novozymes Company) enzymolysis 1h, add again 3 ‰ peptide bond excision enzyme (flavor protease, Guangxi Pang Bo biotechnology) enzymolysis 6h;
(3), be warming up to 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrate weight), the beta-schardinger dextrin-that adds again concentrate weight 10% at concentrated enzymolysis liquid, 90 ℃ of embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains product.
Embodiment 4:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds 10 times of water gagings and mixes;
(2), under 60 ℃, add first 2 ‰ endopeptidases (papain A1, Guangxi Pang Bo biotechnology) enzymolysis 1h, add again 4 ‰ peptide bond excision enzyme (flavor protease, Novozymes Company) enzymolysis 4h;
(3), be warming up to 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrate weight), the converted starch that adds again concentrate weight 10% at concentrated enzymolysis liquid, 90 ℃ of embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains little algae polypeptide powder of the present invention.
Embodiment 5:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds 12 times of water gagings and mixes;
(2), under 55 ℃, add first 2 ‰ endopeptidases (compound protease, Novozymes Company) enzymolysis 1h, add again 4 ‰ peptide bond excision enzyme (flavor protease, Guangxi Pang Bo biotechnology) enzymolysis 4h;
(3), be warming up to 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrate weight), the lactalbumin that adds again concentrate weight 10% at concentrated enzymolysis liquid, 90 ℃ of embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains little algae polypeptide powder of the present invention.
Embodiment 6:The preparation method of little algae polypeptide powder of the present invention may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds 12 times of water gagings and mixes;
(2), under 50 ℃, add first 2 ‰ endopeptidases (compound protease, Novozymes Company) enzymolysis 1h, add again 6 ‰ peptide bond excision enzyme (flavor protease, Novozymes Company) enzymolysis 3h;
(3), be warming up to 95 ℃, 30min is with enzyme-deactivating in insulation, then Vacuum Concentration must concentrate enzymolysis liquid, being concentrated into solid content is that 20%-25%(is that solid content weight is the 20%-25% of concentrate weight), the beta-schardinger dextrin-that adds again concentrate weight 10% at concentrated enzymolysis liquid, 90 ℃ of embeddings 1 hour, 180-200 ℃ of control EAT, leaving air temp 75-85 ℃, spray-drying obtains little algae polypeptide powder of the present invention.
Although the present invention describes with reference to specific embodiment, this description and not meaning that is construed as limiting the present invention.With reference to description of the invention, other of the disclosed embodiments change, and all can expect for those skilled in the art, and this variation should belong in the affiliated claim limited range.
Claims (8)
1. flavouring that contains little algae polypeptide powder is characterized in that being comprised of the component of following weight proportion:
Little algae polypeptide powder 10-20 part, basic flavoring 28-52 part, tasty agents 15-26 part, be flavor agent 6-10 part, spice 2-4 part, filler 0-6.5 part, anticaking agent 0-2 part, seafood essence 0-0.2 part;
Wherein above-mentioned basic flavoring comprises: salt 20-40 part, sucrose 8-12 part, glucose 5-8 part, above-mentioned tasty agents comprises monosodium glutamate 15-25 part, flavour nucleotide disodium 0.5-1 part, above-mentioned be the flavor agent comprise yeast extract 3-5 part and hydrolyzed vegetable protein 3-5 part, above-mentioned spice comprises garlic powder 1-2 part, ginger powder 1-2 part, and above-mentioned filler comprises cornstarch 0-2.3 part and maltodextrin 0-3.8 part.
2. the preparation method of little algae polypeptide powder in the flavouring that contains little algae polypeptide powder described in claim 1 may further comprise the steps:
(1), the algae dregs of rice are pulverized, getting algae dregs of rice powder adds water and mixes;
(2), added protease hydrolyzed 4-7 hour;
(3), with enzyme-deactivating, concentrated enzymolysis liquid then;
(4), add embedding medium, embedding, last spray-drying namely obtain little algae polypeptide powder.
3. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 2 is characterized in that: the addition of above-mentioned water be algae dregs of rice powder weight 8-12 doubly.
4. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 2, it is characterized in that: the hydrolysis temperature of above-mentioned protease is 45-60 ℃.
5. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 2, it is characterized in that: the method for above-mentioned protease hydrolyzed is to adopt endopeptidase and peptide bond excision enzyme to carry out two enzyme substep enzymolysis, wherein said endopeptidase is compound protease or papain, and described peptide bond excision enzyme is flavor protease.
6. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 5, it is characterized in that: the consumption of above-mentioned endopeptidase is the 1-2 ‰ of algae dregs of rice powder weight.
7. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 5, it is characterized in that: the consumption of above-mentioned peptide bond excision enzyme is the 3-6 ‰ of algae dregs of rice powder weight.
8. the preparation method of the little algae polypeptide powder in the described flavouring that contains little algae polypeptide powder according to claim 2, it is characterized in that: above-mentioned embedding medium is beta-schardinger dextrin-, converted starch or lactalbumin.
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| CN102613384B (en) * | 2012-04-18 | 2013-11-06 | 刘锦胜 | Method for preparing spiral seaweed polypeptide powder by using living spiral seaweeds |
| CN103070293A (en) * | 2013-01-22 | 2013-05-01 | 青岛琅琊台集团股份有限公司 | Method for extracting biological protein from aurantiochytrium sp residue |
| CN103880942B (en) * | 2014-03-06 | 2015-12-02 | 福州大学 | A kind of method utilizing enzymolysis protein to prepare metal chelating peptide |
| CN103919255B (en) * | 2014-04-03 | 2015-09-30 | 广东润科生物工程有限公司 | A kind of microcapsule embedded method of protein peptides |
| CN104120160B (en) * | 2014-06-10 | 2017-05-24 | 中国计量学院 | Preparation method for chlorella anti-oxidative peptide |
| CN104256252B (en) * | 2014-09-15 | 2017-01-11 | 福建农林大学 | Method for preparing seaweed product for reducing blood pressure via step-by-step extraction and bioconversion coupling |
| CN105192722A (en) * | 2015-10-27 | 2015-12-30 | 大连雅威特生物技术股份有限公司 | Oligopeptide composition, and delicious seasoning containing same and preparation method and application thereof |
| CN105495509B (en) * | 2015-12-10 | 2018-06-08 | 大连工业大学 | A kind of Undaria pinnatifida Suringar seasoning and its production method |
| CN106333337A (en) * | 2016-08-08 | 2017-01-18 | 大连雅特盐业有限公司 | Seasoning with addition of marine animal and plant product and preparation method thereof |
| CN106755229A (en) * | 2016-11-24 | 2017-05-31 | 福州大学 | A kind of preparation method of schizochytrium limacinum algae cake protein Peptides |
| CN108770882A (en) * | 2018-06-06 | 2018-11-09 | 广西宾德利生物科技有限公司 | Earthworm microalgae small-molecular peptides and the preparation method and application thereof |
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| CN1097961A (en) * | 1994-05-31 | 1995-02-01 | 宁超美 | The manufacture method of nutritious algae liquid |
| CN1125541A (en) * | 1995-12-28 | 1996-07-03 | 广东海洋生物食品有限公司 | Spirulina granule beverage and preparation method thereof |
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Address after: 515041 Guangdong province Shantou Changping Road 90, Suning Plaza Building 2 (8-23), 2106, 2107, 2105, 2108, 2109. Patentee after: Guangdong branch of biological engineering Limited by Share Ltd Address before: 510613 Guangdong city of Shantou Province East Road Longsheng industrial zone Patentee before: Guangdong Runke Bioengineering Co., Ltd. |
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Effective date of registration: 20200825 Address after: 363000 west section of 552 Road, Zhaoan Jindu industrial concentration zone, Zhangzhou City, Fujian Province (National Science and technology Xinghai industrial demonstration base) Patentee after: RUNKE BIOENGINEERING (FUJIAN) Co.,Ltd. Address before: 515041, 2 (8-23) level, 2, 8-23, level 90, Changping Road, Shantou, Guangdong. Patentee before: Guangdong Run Ke bioengineering LLC |