CN101961021A - Fluopyram-containing bactericidal composition - Google Patents
Fluopyram-containing bactericidal composition Download PDFInfo
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- CN101961021A CN101961021A CN201010532504XA CN201010532504A CN101961021A CN 101961021 A CN101961021 A CN 101961021A CN 201010532504X A CN201010532504X A CN 201010532504XA CN 201010532504 A CN201010532504 A CN 201010532504A CN 101961021 A CN101961021 A CN 101961021A
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Abstract
The invention relates to a bactericidal composition in the field of pesticide compounding. The active ingredients of the bactericidal composition are (A) fluopyram and (B) any one of bactericidal active ingredients, namely difenoconazole, hexaconazole, myclobutanil, tricyclazole and mancozeb, wherein the weight percentage ratio of the two active ingredients is 70-1:1-70, preferably 30-5:30-60. The active ingredients account for 5 to 90 percent, preferably 40 to 70 percent of the total mass of the bactericidal composition preparation, and the balance is auxiliary ingredients allowable and acceptable in a pesticide. A known method can be used for preparing wettable powder, cream, suspending agents, water dispersible granules, emulsion in water or microemulsion. The bactericidal composition can be used for preventing and controlling various diseases such as powdery mildew, downy mildew, rust disease, leaf spot disease, venturia inaequalis, rot and the like of crops such as vegetables and wheat and fruit trees.
Description
Technical field
The present invention relates to a kind of Synergistic sterilizing composition pesticide, be specifically related to a kind of fluorine pyrrole bacterium acid amides and composite Synergistic bactericidal composition of arbitrary fungicide active ingredient that is selected from Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole, the mancozeb of comprising.
Background technology
Fluorine pyrrole bacterium acid amides, English common name fluopyram, chemical name is N-{2-[3-chloro-5-(trifluoromethyl)-2-pyridine radicals] ethyl-α, α, α-three fluoro-O-toluamide }, be a kind of new benzamides series bactericidal agent, shift by the electronics that hinders succinate dehydrogenase in the respiratory chain and suppress mitochondrial respiratory.Be mainly used in the microbial disease of control capsule on the broad leaf crop.To the downy mildew on the vegetables such as cucumber, custard squash, powdery mildew, the downy mildew of fruit tree etc. causes that by germ multiple diseases has favorable effect.Its chemical structural formula is:
Difenoconazole (Difenoconazole) also Cheng Difenoconazole; belong to triazole bactericidal agent; it is a kind of interior wide-spectrum bactericide of inhaling; have good protection and therapeutic action simultaneously; be sterol demethylation inhibitor, Ascomycetes, Basidiomycetes, imperfect fungus, Oomycete are had lasting protection and therapeutic activity.As diseases such as leaf spot, powdery mildew, rust and scab preventive effect is preferably arranged to various vegetables and fruit tree.
Own azoles alcohol (hexaconazole) is the triazole bactericidal agent of Syngenta Co.,Ltd's exploitation.Molecular formula: C
14H
17Cl
2N
3O, chemical name: (RS)-2-(2,4 dichloro benzene base)-1-(1H-1,2,4-triazol-1-yl)-oneself-2-alcohol.Own azoles alcohol belongs to sterol demethylation inhibitor, destroys and stop the biosynthesis of the cell membrane important composition composition ergosterol of germ, causes cell membrane not form, and makes germ death.Suction in the tool, protection and therapeutic activity all have good control efficiency to powdery mildew, rust, tikka class disease and gray mold etc.
Nitrile bacterium azoles (myclobutanil), molecular formula: C
15H
17ClN
4, chemical formula is: the own nitrile of 2-(4-chlorphenyl)-2-(1H, 1,2,4-triazole-1-methyl).Nitrile bacterium azoles is a kind of absorption triazole bactericidal agent in protection and the therapeutic action that has, and the biosynthesis of main ergosterol to pathogen plays inhibitory action, and sac fungi, basidiomycetes are all had the better prevention effect.This agent lasting period is long, to crop safety, certain stimulating growth effect is arranged.Mainly be applicable to the powdery mildew, scab, rot, rust of control fruit tree and vegetable, ornamental crops etc.
Tricyclazole has another name called than gorgeous (beam), Tricyclazole; chemical name is the 5-methyl isophthalic acid; 2; the 4-triazol (3,4-b) benzothiazole, interior absorption is strong; low toxicity; belong to Synthetic inhibitor of melanin (MBIs), in agricultural production, can only can be used to soak root or foliar spray and handle at present as the protectant of rice blast.Tricyclazole is the main medicament of present China control rice blast; though studies show that rice blast is lower to the pesticide resistance risk of tricyclazole; but there is a main deficiency in this medicament: have only protective effect, and without any therapeutic action, promptly can only medication before rice blast infects generation.In case after infecting or rice leaf cause the paddy rice wound to prevent and treat with tricyclazole more later on because of wind and rain or other reasons, will not have protection effect.
Mancozeb (mancozeb) molecular formula: C
4H
6N
2S
4The MnZn chemical name: the complex salt of two two thiocarbamoyl manganese of ethylene and zinc is the complex that maneb and zinc are formed, and belongs to the wider protective fungicide of fungicidal spectrum.It mainly is the oxidation that suppresses pyruvic acid in the thalline.Effective to multiple diseases such as the anthracnose on fruit tree, the vegetables, early blight, simultaneously its normal and systemic fungicide is mixed, and is used for the generation of delaying drug resistance.
Above bactericide is long-term to be used separately, and preventive effect begins to descend, and has produced pesticide resistance to a certain degree, and simultaneously, above bactericide has in various degree inhibitory action to the growth of crop when high dose uses slightly, cause poisoning easily.The inventor discovers by a series of, fluorine pyrrole bacterium acid amides be selected from triazole type (Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole) and the mancozeb arbitrary fungicide active ingredient be mixed the target bacterium had extremely strong synergistic effect, improve bactericidal activity greatly, thereby finished the present invention.And about fluorine pyrrole bacterium acid amides and be selected from triazole type (Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole) and the mancozeb arbitrary fungicide active ingredient composite, do not see relevant report at present at home and abroad as yet.
Summary of the invention
The purpose of this invention is to provide the low bactericidal composition of a kind of efficient, low toxicity, use cost to satisfy the needs of agricultural production.Two kinds of active component combination backs produce synergistic effect, can also avoid the drug-fast generation of germ and delay the drug-fast generation speed of germ when improving preventive effect.
The objective of the invention is to realize by following measure:
A kind of bactericidal composition based on fluorine pyrrole bacterium acid amides, it is characterized in that with active ingredient (A) fluorine pyrrole bacterium acid amides and (B) the arbitrary fungicide active ingredient in a kind of triazole class compounds or the mancozeb carry out compositely, the percentage by weight of effective constituents A and B is 70~1: 1~70.
Triazole class compounds of the present invention is: Difenoconazole, own azoles alcohol, nitrile bacterium azoles and tricyclazole.
Above-mentioned triazole class compounds and mancozeb all are bactericide well known in the art, can obtain by various commercial channel, and as a kind of improvement of the present invention, the percentage by weight of effective constituents A and B preferred 30~5: 30~60.
Except active ingredient (A) fluorine pyrrole bacterium acid amides with (B) a kind of triazole class compounds or the mancozeb, comprise also in the composition of the present invention that auxiliary agent such as solvent, dispersant, emulsifier, stabilizing agent, antifreezing agent, wetting agent and other are of value to the known substance of active ingredient stable and drug effect performance in preparation, it all is the auxiliary component of using or allow use in the pesticidal preparations always, be not particularly limited, concrete composition and consumption require to determine by simple experiment according to form formula.
Bactericidal composition based on fluorine pyrrole bacterium acid amides of the present invention can be prepared into the suitable agriculture formulation of using with known method and be wetting powder, missible oil, suspending agent, water dispersible granules, aqueous emulsion or microemulsion, and the quality percentage composition of active ingredient is 5%~90% in the preparation.
Bactericidal composition based on fluorine pyrrole bacterium acid amides of the present invention can be used for preventing and treating diseases caused by the bacteria such as powdery mildew on cereal such as various vegetables, wheat and the fruit tree, downy mildew, rust, tikka class disease, scab, rot.Compared with prior art, beneficial effect of the present invention is: 1. composite have an obvious role in synergism, improved control efficiency, enlarged fungicidal spectrum; 2. compare with using the one-component bactericide, the risk that produces poisoning reduces; 3. composition is made up of two kinds of different active ingredients of the mechanism of action, helps avoiding drug-fast generation and delays the drug-fast generation speed of germ; 4. medicament is mixed and has reduced dosage, thereby has reduced cost and the pollution that has alleviated environment.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of being made within the spirit and principles in the present invention, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Indoor bioassay:
(1) experimental subjects: the powdery mildew of cucumber bacterium, the cucumber cultivation ground collection of not executing fluorine pyrrole bacterium amine and Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole, mancozeb and similar medicament from Shouguang, Shandong separates, and kind is a Chang Chun Mi Ci.
In pot for growing seedlings, place the greenhouse to cultivate susceptible cucumber variety Chang Chun Mi Ci cultivation, plant grows to three to four leaf after dates, gathers the blade of identical leaf age, is used for cultivation and the mensuration of powdery mildew of cucumber bacterium.
The powdery mildew of cucumber bacterium adopts live body plant method to cultivate under 20 ℃, the condition of 12h alternation of light and darkness, and every 30d changes to be commissioned to train and supports 1 time.With the conidium on the sterile water wash-out incidence of leaf, being mixed with spore concentration is 1 * 10 during inoculation
6The suspension of individual/mL.
(2) experimental pharmacy: the former medicine of 96% fluorine pyrrole bacterium acid amides, produce by Jiangsu Heyi Chemical Industry Co., Ltd.; 95% difenoconazole technical material, Jiangsu Qizhou Green Chemical Co., Ltd produces; The former medicine of 95% own azoles alcohol, Jiangsu Qizhou Green Chemical Co., Ltd produces; 95% bulk myclobutanil, Zhejiang Province Yifan Chemical Co., Ltd produces; The former medicine of 95% tricyclazole, the new chemical industry of Hangzhou standing grain Co., Ltd produces; The former medicine of 90% mancozeb, the eternal chemical industry in Shanghai Co., Ltd produces.
(3) test method: adopt the leaf dish method of preserving moisture to carry out toxicity test.Earlier the blade of gathering is prepared into the leaf dish that diameter is 1.5cm, mixing at random, place the series concentration soup that configures to soak 1h respectively, 50 leaf dishes of each concentration, test is with the blank that is treated to of adding medicine not, after soaking end, leaf faces up and puts on the wetting blotting paper of identical liquor strength, soup on the leaf dish is blotted, the spore suspension 10L for preparing is inoculated in leaf dish central authorities, after room temperature is placed 5min, places under 20 ℃, the condition of 12h alternation of light and darkness and cultivate, measure the onset area on the leaf dish behind the 10d, calculate EC
50
The percentage that accounts for the leaf disc area according to lesion area is divided sick level:
0 grade: scab
1 grade: lesion area accounts for 1%~20% of leaf disc area
2 grades: lesion area accounts for 21%~50% of leaf disc area
3 grades: lesion area accounts for more than 50% of leaf disc area
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) * 100
Content (%) CTC=(ATI/TTI) * 100 of the toxicity index X B of content (the %)+B of the toxicity index x A of TI I=A in using with in using with
If co-toxicity coefficient is big by 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100~120, be indicated as summation action.
Biologicall test example 1: the composite Toxicity Determination of fluorine pyrrole bacterium acid amides and Difenoconazole to the powdery mildew of cucumber bacterium.
The soup preparation of single agent toxicity test: fluorine pyrrole bacterium acid amides and difenoconazole technical material acetone are dissolution with solvents, are made into 10000 μ g/ml mother liquors, and is in 4 ℃ of preservations, stand-by.
Combination setting is mixed: 55% fluorine pyrrole bacterium acid amides Difenoconazole mass ratio is respectively 4.58%+50.42%, 5.5%+49.5%, 9.17%+45.83%, 13.75%+41.25% and 27.5%+27.5% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single agent fluorine pyrrole bacterium acid amides and Difenoconazole are carried out toxicity test, the two EC in order to last method
50Value is respectively 1.6mg/l and 3.5mg/l.
Virulence experiment result and analysis:
The different proportioning fluorine of table 1 pyrrole bacterium acid amides and Difenoconazole mixture are to the virulence of powdery mildew of cucumber bacterium
The composite co-toxicity of table 2 fluorine pyrrole bacterium acid amides and Difenoconazole to the powdery mildew of cucumber bacterium
Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
Fluorine pyrrole bacterium acid amides (A) | 1∶0? | 100.00? | 100.00? | /? | /? |
Difenoconazole (B) | 0∶1? | 45.71? | 45.71? | /? | /? |
?A+B? | 1∶11? | 27.57? | 27.63? | 99.8? | Addition |
?A+B? | 1∶9? | 31.11? | 28.13? | 110.6? | Addition |
?A+B? | 1∶5? | 34.91? | 30.12? | 115.9? | Addition |
?A+B? | 1∶3? | 43.86? | 32.61? | 134.5? | Synergy |
?A+B? | 1∶1? | 44.88? | 40.07? | 112.0? | Addition |
According to bactericide joint toxicity measuring result, fluorine pyrrole bacterium acid amides Difenoconazole mass ratio is that 1: 3 o'clock co-toxicity coefficient is 134.5, greater than 120, shows as synergistic effect, and other show as summation action greater than 100, thinks 1: 3 and is best proportioning.
Biologicall test example 2: fluorine pyrrole bacterium acid amides and the composite Toxicity Determination of own azoles alcohol to the powdery mildew of cucumber bacterium.
The soup preparation of single agent toxicity test: with fluorine pyrrole bacterium acid amides and the former medicinal acetone of own azoles alcohol is dissolution with solvents, is made into 10000 μ g/ml mother liquors, in 4 ℃ of preservations, stand-by.
Combination setting is mixed: the own azoles alcohol of 56% fluorine pyrrole bacterium acid amides mass ratio is respectively 4.7%+51.3%, 5.6%+50.4%, 9.3%+46.7%, 14%+42% and 28%+28% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single agent fluorine pyrrole bacterium acid amides and own azoles alcohol are carried out toxicity test, the two EC in order to last method
50Value is respectively 1.5mg/l and 5.6mg/l
Virulence experiment result and analysis:
The different proportioning fluorine of table 3 pyrrole bacterium acid amides and own azoles alcohol mixture are to virulence and the synergistic effect of powdery mildew of cucumber bacterium
Table 4 fluorine pyrrole bacterium acid amides and the composite co-toxicity of own azoles alcohol to the powdery mildew of cucumber bacterium
According to bactericide joint toxicity measuring result, the own azoles alcohol of fluorine pyrrole bacterium acid amides mass ratio is that 1: 3 o'clock co-toxicity coefficient is 144.2, greater than 120, shows as synergistic effect, and other show as summation action greater than 100, think 1: 3 and are best proportioning.
Biologicall test example 3: fluorine pyrrole bacterium acid amides and myclobutanil compositional are to the Toxicity Determination of powdery mildew of cucumber bacterium.
The soup preparation of single agent toxicity test: with fluorine pyrrole bacterium acid amides and bulk myclobutanil acetone is dissolution with solvents, is made into 10000 μ g/ml mother liquors, in 4 ℃ of preservations, stand-by.
Combination setting is mixed: 56% fluorine pyrrole bacterium acid amides nitrile bacterium azoles mass ratio is respectively 4.7%+51.3%, 5.6%+50.4%, 9.3%+46.7%, 14%+42% and 28%+28% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single agent fluorine pyrrole bacterium acid amides and nitrile bacterium azoles are carried out toxicity test, the two EC in order to last method
50Value is respectively 1.9mg/l and 7.5mg/l.
Virulence experiment result and analysis:
The different proportioning fluorine of table 5 pyrrole bacterium acid amides and nitrile bacterium azoles mixture are to virulence and the synergistic effect of powdery mildew of cucumber bacterium
Table 6 fluorine pyrrole bacterium acid amides and myclobutanil compositional are to the co-toxicity of powdery mildew of cucumber bacterium
Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
Fluorine pyrrole bacterium acid amides (A) | 1∶0? | 100.00? | 100.00? | /? | /? |
Nitrile bacterium azoles (B) | 0∶1? | 25.33? | 25.33? | /? | /? |
?A+B? | 1∶11? | 17.68? | 17.70? | 99.9? | Addition |
A+B? | 1∶9? | 20.22? | 18.37? | 110.1? | Addition |
A+B? | 1∶5? | 27.45? | 21.13? | 129.9? | Synergy |
A+B? | 1∶3? | 27.40? | 24.64? | 111.2? | Addition |
A+B? | 1∶1? | 37.06? | 35.09? | 105.6? | Addition |
According to bactericide joint toxicity measuring result, fluorine pyrrole bacterium acid amides nitrile bacterium azoles mass ratio is that 1: 5 o'clock co-toxicity coefficient is 129.9, greater than 120, shows as synergistic effect, and other show as summation action greater than 100, thinks 1: 5 and is best proportioning.
Biologicall test example 4: the composite Toxicity Determination of fluorine pyrrole bacterium acid amides and tricyclazole to the powdery mildew of cucumber bacterium.
The soup preparation of single agent toxicity test: with fluorine pyrrole bacterium acid amides and the former medicinal acetone of tricyclazole is dissolution with solvents, is made into 10000 μ g/ml mother liquors, in 4 ℃ of preservations, stand-by.
Combination setting is mixed: 60% fluorine pyrrole bacterium acid amides tricyclazole mass ratio is respectively 5%+55%, 6%+54%, 10%+50%, 15%+45% and 30%+30% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single agent fluorine pyrrole bacterium acid amides and tricyclazole are carried out toxicity test, the two EC in order to last method
50Value is respectively 0.7mg/l and 8.3mg/l.
Virulence experiment result and analysis:
The different proportioning fluorine of table 7 pyrrole bacterium acid amides and tricyclazole mixture are to virulence and the synergistic effect of powdery mildew of cucumber bacterium
The composite co-toxicity of table 8 fluorine pyrrole bacterium acid amides and tricyclazole to the powdery mildew of cucumber bacterium
Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
Fluorine pyrrole bacterium acid amides (A) | 1∶0? | 100.00? | 100.00? | /? | /? |
Tricyclazole (B) | 0∶1? | 8.43? | 8.43? | /? | /? |
?A+B? | 1∶11? | 10.05? | 9.64? | 104.3? | Addition |
?A+B? | 1∶9? | 12.11? | 10.55? | 114.7? | Addition |
?A+B? | 1∶5? | 21.13? | 14.22? | 148.6? | Synergy |
?A+B? | 1∶3? | 22.22? | 18.80? | 118.2? | Addition |
A+B? | 1∶1? | 34.38? | 32.53? | 105.7? | Addition |
According to bactericide joint toxicity measuring result, fluorine pyrrole bacterium acid amides tricyclazole mass ratio is that 1: 5 o'clock co-toxicity coefficient is 148.6, greater than 120, shows as synergistic effect, and other show as summation action greater than 100, thinks 1: 5 and is best proportioning.
Biologicall test example 5: the composite Toxicity Determination of fluorine pyrrole bacterium acid amides and mancozeb to the powdery mildew of cucumber bacterium.
The soup preparation of single agent toxicity test: former medicinal acetone is dissolution with solvents, is made into 10000 μ g/ml mother liquors, and is in 4 ℃ of preservations, stand-by.
Combination setting is mixed: 60% fluorine pyrrole bacterium acid amides mancozeb mass ratio is respectively 5%+55%, 6%+54%, 10%+50%, 15%+45% and 30%+30% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single agent fluorine pyrrole bacterium acid amides and mancozeb are carried out toxicity test, the two EC in order to last method
50Value is respectively 1.2mg/l and 7.8mg/l.
Virulence experiment result and analysis:
The different proportioning fluorine of table 9 pyrrole bacterium acid amides and mancozeb mixture are to the virulence of powdery mildew of cucumber bacterium
The composite co-toxicity of table 10 fluorine pyrrole bacterium acid amides and mancozeb to the powdery mildew of cucumber bacterium
Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
Fluorine pyrrole bacterium acid amides (A) | 1∶0? | 100.00? | 100.00? | /? | /? |
Mancozeb (B) | 0∶1? | 15.38? | 15.38? | /? | /? |
?A+B? | 1∶11? | 15.39? | 13.46? | 114.3? | Addition |
?A+B? | 1∶9? | 19.59? | 14.31? | 136.9? | Synergy |
?A+B? | 1∶5? | 20.43? | 17.69? | 115.5? | Addition |
?A+B? | 1∶3? | 23.85? | 21.92? | 108.8? | Addition |
?A+B? | 1∶1? | 34.58? | 34.62? | 99.9? | Addition |
According to bactericide joint toxicity measuring result, fluorine pyrrole bacterium acid amides mancozeb mass ratio is that 1: 9 o'clock co-toxicity coefficient is 136.9, greater than 120, shows as synergistic effect, and other show as summation action, is best proportioning so think 1: 9.
Field trial:
Test example 1: field trial is measured fluorine pyrrole bacterium acid amides and the composite control efficiency to powdery mildew of cucumber and custard squash powdery mildew of Difenoconazole
(1) investigation method
Experimental field be located in Shandong Province's Qingzhou City cucumber booth, in Shandong Province's Qingzhou City custard squash booth, 5 points are investigated in every sub-district, and every some investigation 2 strains are put down in writing occurring degree with staging.
Grade scale is as follows:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for whole leaf area 6%~10%;
5 grades: lesion area accounts for 11%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for whole more than 50% of leaf area.
(2) control time and number of times
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 7 days behind the 1st medicine, 7 days and preventive effect investigation in 15 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) result of the test and analysis
Each handles the effect of preventing and treating powdery mildew of cucumber table 11
As can be seen from Table 11, to prevent and treat the effect of powdery mildew of cucumber remarkable in the combination that is mixed of 55% fluorine pyrrole bacterium acid amides Difenoconazole SC 1: 3.Under the consumption of same every mu 9 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 76.02% and 82.58%, the 2 medicine respectively, reaches 83.98%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 10% Difenoconazole WG.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 77.39%, 10% Difenoconazole WG is 69.42%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to cucumber all do not occur, illustrates that it is safe to cucumber.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
Each handles the effect of control custard squash powdery mildew table 12
As can be seen from Table 12, the effect of 1: 3 the combination control custard squash powdery mildew that is mixed of 55% fluorine pyrrole bacterium acid amides Difenoconazole SC is remarkable.Under the consumption of same every mu 9 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 69.05.76% and 80.77%, the 2 medicine respectively, reaches 84.44%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 10% Difenoconazole WG.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 81.10%, 10% Difenoconazole WG is 78.99%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to custard squash all do not occur, illustrates that it is safe to custard squash.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
Test example 2: field trial measure fluorine pyrrole bacterium acid amides respectively with own azoles alcohol, nitrile bacterium azoles, tricyclazole, the single composite control efficiency of mancozeb to powdery mildew of cucumber and custard squash powdery mildew
(1) investigation method
Except that experimental field being located at the Yangling District Li Jia mausoleum, Shaanxi with the composite of nitrile bacterium azoles, the composite field trial of its excess-three kind compound and fluorine pyrrole bacterium acid amides all is located at the cucumber ground in Yangling District Li Tai township, Shaanxi, in the custard squash booth, 5 points are investigated in every sub-district, every some investigation 2 strains, put down in writing occurring degree with staging, grade scale is as follows:
0 grade: no scab;
1 grade: lesion area accounts for whole below 5% of leaf area;
3 grades: lesion area accounts for whole leaf area 6%~10%;
5 grades: lesion area accounts for 11%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for whole more than 50% of leaf area.
(2) control time and number of times
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 7 days behind the 1st medicine, 7 days and preventive effect investigation in 15 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) result of the test and analysis
1. fluorine pyrrole bacterium acid amides and own azoles alcohol are composite:
Each handles the effect of preventing and treating powdery mildew of cucumber table 13
As can be seen from Table 13, to prevent and treat the effect of powdery mildew of cucumber remarkable in the combination that is mixed of the own azoles alcohol of 56% fluorine pyrrole bacterium acid amides 1: 3.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 68.67% and 77.97%, the 2 medicine respectively, reaches 83.78%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 30% own azoles alcohol SC.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 75.99%, 30% own azoles alcohol SC is 72.98%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to cucumber all do not occur, illustrates that it is safe to cucumber.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
Each handles the effect of control custard squash powdery mildew table 14
As can be seen from Table 14, as can be seen from Table 4, the effect of 1: 3 the combination control custard squash powdery mildew that is mixed of the own azoles alcohol of 56% fluorine pyrrole bacterium acid amides SC is remarkable.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 70.88% and 79.67%, the 2 medicine respectively, reaches 85.01%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 30% own azoles alcohol SC.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 79.83%, 30% own azoles alcohol SC is 74.86%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to custard squash all do not occur, illustrates that it is safe to custard squash.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
2. fluorine pyrrole bacterium acid amides and myclobutanil compositional:
Each handles the effect of preventing and treating powdery mildew of cucumber table 15
As can be seen from Table 15, to prevent and treat the effect of powdery mildew of cucumber remarkable in the combination that is mixed of 56% fluorine pyrrole bacterium acid amides nitrile bacterium azoles 1: 5.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 73.76% and 78.86%, the 2 medicine respectively, reaches 83.71%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 40% nitrile bacterium azoles SC.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 78.07%, 40% nitrile bacterium azoles SC is 72.53%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to cucumber all do not occur, illustrates that it is safe to cucumber.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
Each handles the effect of control custard squash powdery mildew table 16
As can be seen from Table 16, as can be seen from Table 4, the effect of 1: 5 the combination control custard squash powdery mildew that is mixed of 56% fluorine pyrrole bacterium acid amides nitrile bacterium azoles SC is remarkable.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 76.01% and 82.92%, the 2 medicine respectively, reaches 85.60%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 40% nitrile bacterium azoles SC.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 81.20%, 40% nitrile bacterium azoles SC is 78.33%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to custard squash all do not occur, illustrates that it is safe to custard squash.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
3. fluorine pyrrole bacterium acid amides and tricyclazole are composite:
Each handles the effect of preventing and treating powdery mildew of cucumber table 17
As can be seen from Table 17, to prevent and treat the effect of powdery mildew of cucumber remarkable in the combination that is mixed of 60% fluorine pyrrole bacterium acid amides tricyclazole 1: 5.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 75.21% and 83.01%, the 2 medicine respectively, reaches 86.23%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 75% tricyclazole WP.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 80.65%, 75% tricyclazole WP is 78.96%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to cucumber all do not occur, illustrates that it is safe to cucumber.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
Each handles the effect of control custard squash powdery mildew table 18
As can be seen from Table 18, as can be seen from Table 4, the effect of 1: 5 the combination control custard squash powdery mildew that is mixed of 60% fluorine pyrrole bacterium acid amides tricyclazole SC is remarkable.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 77.65% and 83.72%, the 2 medicine respectively, reaches 86.91%.Result of the test proves that the single agent effect of 41.7% fluorine pyrrole bacterium acid amides SC obviously is better than the single agent of 75% tricyclazole WP.Behind the 2nd medicine 15 days, the highest preventive effect of the single agent of 41.7% fluorine pyrrole bacterium acid amides SC was that the highest preventive effect of the single agent of 83.01%, 75% tricyclazole WP is 80.60%.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to custard squash all do not occur, illustrates that it is safe to custard squash.Prevent desirable even more ideal control efficiency at the initial stage of a disease or in advance.
4. fluorine pyrrole bacterium acid amides and mancozeb are composite:
Each handles the effect of preventing and treating powdery mildew of cucumber table 19
As shown in Table 19, fluorine pyrrole bacterium acid amides and the mancozeb effect for powdery mildew of cucumber under 1: 9 proportioning is remarkable; Behind its 1 time, 2 times medicines behind 7 days and 2 medicines 15 days preventive effect significantly be better than two kinds of single agent, illustrate that complex preparation has significant synergistic effect, not only quick-acting is better for germ for it, and lasting effect is also very remarkable; In addition, through field trial checking, the agent of fluorine pyrrole bacterium acid amides list is better than the agent of mancozeb list for the preventive effect of powdery mildew of cucumber, and complex preparation and two kinds of single agent all in the using dosage scope to crop safety, have no adverse effects.
Each handles the effect of control custard squash powdery mildew table 20
As shown in Table 20, the effect of 1: 9 the combination control custard squash powdery mildew that is mixed of fluorine pyrrole bacterium acid amides mancozeb is remarkable.Under the consumption of same every mu 10 gram, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and be the most outstanding with lasting effect especially.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.15 days lasting effect is more remarkable after 7 days control efficiency behind the 1st, 2 medicine reaches 68,69% and 80,87%, the 2 medicine respectively, reaches 83.92%.Result of the test proves that 41.7% fluorine pyrrole bacterium acid amides list agent effect obviously is better than the single agent of 80% mancozeb WP.According to field range estimation, in the test dose scope, plant growth is normal, and each is handled medicament and poisoning phenomenon to custard squash all do not occur, illustrates that it is safe to custard squash.
In sum, the present invention contains the fluorine pyrrole bacterium acid amides Synergistic bactericidal composition composite with the arbitrary fungicide active ingredient that is selected from Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole, Mancozeb, powdery mildew of cucumber and Pumpkin powdery mildew there is good prevention effect, and it is to the target crop safety, compare with single dose, bactericidal composition of the present invention has the advantage that quick-acting is good, the lasting period is long, is difficult for producing poisoning. So research and development of the present invention and popularization are of great significance social tool.
Claims (7)
1. bactericidal composition is characterized in that: active ingredient is composite with any fungicide active ingredient that (B) is selected from Difenoconazole, own azoles alcohol, nitrile bacterium azoles, tricyclazole, the mancozeb for (A) fluorine pyrrole bacterium acid amides.
2. bactericidal composition according to claim 1 is characterized in that: wherein the percentage by weight of effective constituents A and B is 70~1:1~70.
3. bactericidal composition according to claim 1 is characterized in that: wherein the percentage by weight of effective constituents A and B is preferably 30~5:30~60.
4. bactericidal composition according to claim 1, it is characterized in that: except effective constituents A and B, also comprise known auxiliary agents such as solvent, dispersant, emulsifier, stabilizing agent, antifreezing agent, wetting agent in the preparation of composition of the present invention, the quality percentage composition of active ingredient is 5%~90% in the preparation.
5. bactericidal composition according to claim 1 is characterized in that: the quality percentage composition of active ingredient is preferably 40%~70% in the preparation.
6. bactericidal composition according to claim 4 is characterized in that: the formulation that described bactericidal composition can be made into is wetting powder, missible oil, suspending agent, water dispersible granules, aqueous emulsion or microemulsion.
7. bactericidal composition according to claim 1 is characterized in that: the application of described bactericidal composition is used to prevent and treat diseases caused by the bacteria such as powdery mildew on cereal such as various vegetables, wheat and the fruit tree, downy mildew, rust, tikka class disease, scab, rot.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102027934A (en) * | 2010-11-26 | 2011-04-27 | 惠州市中迅化工有限公司 | Bactericidal composition containing fluopyram and difenoconazole and application thereof |
CN103518741A (en) * | 2012-07-06 | 2014-01-22 | 陕西美邦农药有限公司 | Fluopyram-containing high-efficiency bactericidal composition |
CN104170829A (en) * | 2014-09-01 | 2014-12-03 | 山东碧奥生物科技有限公司 | Pesticide composition containing fluopyram and difenoconazole |
CN109566631A (en) * | 2017-09-29 | 2019-04-05 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
CN110710534A (en) * | 2018-07-13 | 2020-01-21 | 江苏龙灯化学有限公司 | Application of sterilization composition containing fluopyram and mancozeb |
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WO2005077183A1 (en) * | 2004-02-12 | 2005-08-25 | Bayer Cropscience Sa | Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the ergosterol biosynthesis |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2005077183A1 (en) * | 2004-02-12 | 2005-08-25 | Bayer Cropscience Sa | Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the ergosterol biosynthesis |
CN1917760A (en) * | 2004-02-12 | 2007-02-21 | 拜尔农科股份有限公司 | Fungicidal composition comprising a pyridylethylbenzamide derivative and a compound capable of inhibiting the ergosterol biosynthesis |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102027934A (en) * | 2010-11-26 | 2011-04-27 | 惠州市中迅化工有限公司 | Bactericidal composition containing fluopyram and difenoconazole and application thereof |
CN103518741A (en) * | 2012-07-06 | 2014-01-22 | 陕西美邦农药有限公司 | Fluopyram-containing high-efficiency bactericidal composition |
CN104170829A (en) * | 2014-09-01 | 2014-12-03 | 山东碧奥生物科技有限公司 | Pesticide composition containing fluopyram and difenoconazole |
CN109566631A (en) * | 2017-09-29 | 2019-04-05 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
CN110710534A (en) * | 2018-07-13 | 2020-01-21 | 江苏龙灯化学有限公司 | Application of sterilization composition containing fluopyram and mancozeb |
CN110710534B (en) * | 2018-07-13 | 2021-06-15 | 江苏龙灯化学有限公司 | Application of sterilization composition containing fluopyram and mancozeb |
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