CN101946634A - Manufacturing method for morchella mother culture - Google Patents
Manufacturing method for morchella mother culture Download PDFInfo
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- CN101946634A CN101946634A CN2010102794055A CN201010279405A CN101946634A CN 101946634 A CN101946634 A CN 101946634A CN 2010102794055 A CN2010102794055 A CN 2010102794055A CN 201010279405 A CN201010279405 A CN 201010279405A CN 101946634 A CN101946634 A CN 101946634A
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- bonatii
- populus
- leachate
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Abstract
The invention provides a manufacturing method for morchella mother culture. The method is characterized by comprising the following steps of: 1, boiling 1 to 3 parts of fresh insect-free populus bonatii smashed branches or populus bonatii leaves in 10 parts of water based on a mass ratio for 25 to 30 minutes, filtering the mixture, and taking juice to prepare populus bonatii leachate; 2, mixing the populus bonatii leachate and PDA culture solution in a mass ratio of 1: 1, adding 0.2 percent of Na3PO4.12H2O, 0.2 percent of KH2PO4, 0.05 percent of MgSO4 and 0.005 percent of VB1 based on the mass percent of the mixed liquor, uniformly stirring the mixture, and sterilizing the mixture at the temperature of 121 +/1 DEG C for 30 minutes to prepare a culture medium; and 3, cooling the culture medium, inoculating the morchella mother culture, culturing the inoculated culture medium at the constant temperature of 23 DEG C for 10 to 12 days, and culturing the culture medium in an incubator at the temperature of between 8 and 12 DEG C for 4 to 6 days. The manufacturing method has the advantages of strong hypha, strong twisting force, and easy formation of sclerotium on protospecies or cultivar.
Description
Technical field:
The present invention relates to the female preparation method of planting of a kind of hickory chick.
Background technology:
Hickory chick is that a kind of famous and precious food (medicine) is used bacterium, has begun bionical domesticating and cultivating at present.Female the manufacturing of planting of hickory chick is the first step of bionical domesticating and cultivating, also is the key link.The female kind of hickory chick is mother culture media with the PDA medium generally at present, the mycelia of this mother culture media shows characteristics such as mycelia is very thin, kink power is not strong on test tube or culture dish, original seed or the also more very thin and difficult formation sclerotium of cultivated species mycelia with this mother culture media is made cause production ratio lower.Therefore, research a kind of female plant healthy and strong, kink power is strong and the female kind of the high-quality preparation method that can form sclerotium on original seed or cultivated species has great facilitation to the raising output of hickory chick.
Summary of the invention:
The present invention seeks to solve the problem that the female kind system method of above-mentioned existing hickory chick exists, a kind of mycelia stalwartness is provided, kink power is strong and on original seed or cultivated species, forms the female preparation method of planting of hickory chick of sclerotium easily.
The present invention is achieved in that
1, the water of 1～3 part the fresh broken branch of free from insect pests roundleaf poplar or roundleaf Poplar leaves being put into 10 parts by mass ratio boils 25～30min, crosses leaching juice and makes roundleaf poplar leachate.
2, after roundleaf poplar leachate and PDA culture fluid are pressed mass ratio 1: 1 and mixed, add Na again by the mass percent of mixed liquor
3PO
412H
2O0.2%, KH
2PO
40.2%, MgSO
40.05%, V
B10.005%, medium is made at 121 ± 1 ℃ of 30min that sterilize down in the back that stirs.
3, connect after the medium cooling that hickory chick is female to be planted, the medium that connects kind after cultivating 10～12d under 23 ℃ of constant temperature, is put into 8～12 ℃ incubator again and cultivated 4～6d, the female kind of hickory chick promptly completes.
The present invention can make and show on the hickory chick mother culture media that the mycelial growth leading portion is neat, bacterium colony is rounded, radial spreading, the characteristics that nucleated mycelium is many, kink power is strong, on original seed or cultivated species, continue these characteristics again equally and can become 30% sclerotium than common hickory chick bacterial classification multiform, thereby the output than existing hickory chick bacterial classification plantation gained improves 15% on output, thereby the domesticating and cultivating of hickory chick there is obvious facilitation.
Further specify particular content of the present invention below in conjunction with embodiment.
Embodiment
Embodiment
1, selects for use the fresh free from insect pests roundleaf of 200g poplar branch to be cut into 1～2cm segment or pulverizing 100g prophyll poplar dried leaf, add in the 1L water and boil 25～30min, cross leaching juice and make roundleaf poplar leachate.
2, after roundleaf poplar leachate and PDA culture fluid are pressed mass ratio 1: 1 and mixed, add Na again by the mass percent of mixed liquor
3PO
412H
2O0.2%, KH
2PO
40.2%, MgSO
40.05%, V
B10.005%, medium is made at 121 ± 1 ℃ of 30min that sterilize down in the back that stirs.
3, connect after the medium cooling that hickory chick is female to be planted, the medium that connects kind after cultivating 11d under 23 ℃ of constant temperature, is put into 10 ℃ incubator again and cultivated 5d, the female kind of hickory chick promptly completes.
Claims (1)
1. a hickory chick mother plants preparation method, it is characterized in that:
(1) water of 1～3 part the fresh broken branch of free from insect pests roundleaf poplar or roundleaf Poplar leaves being put into 10 parts by mass ratio boils 25～30min, crosses leaching juice and makes roundleaf poplar leachate;
(2) after roundleaf poplar leachate and PDA culture fluid are pressed mass ratio 1: 1 and mixed, add Na again by the mass percent of mixed liquor
3PO
412H
2O0.2%, KH
2PO
40.2%, MgSO
40.05%, V
B10.005%, medium is made at 121 ± 1 ℃ of 30min that sterilize down in the back that stirs;
(3) connect the female kind of hickory chick after the medium cooling, the medium that connects kind after cultivating 10～12d under 23 ℃ of constant temperature, is put into 8～12 ℃ incubator again and cultivated 4～6d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102794055A CN101946634A (en) | 2010-09-13 | 2010-09-13 | Manufacturing method for morchella mother culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102794055A CN101946634A (en) | 2010-09-13 | 2010-09-13 | Manufacturing method for morchella mother culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101946634A true CN101946634A (en) | 2011-01-19 |
Family
ID=43450358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102794055A Pending CN101946634A (en) | 2010-09-13 | 2010-09-13 | Manufacturing method for morchella mother culture |
Country Status (1)
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| CN (1) | CN101946634A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823429A (en) * | 2012-09-03 | 2012-12-19 | 朱斗锡 | Novel morel cultivation method |
| CN103688745A (en) * | 2013-12-02 | 2014-04-02 | 兴化市板桥食用菌有限公司 | Pholiota adiposa cultivating method |
| CN107653194A (en) * | 2017-11-14 | 2018-02-02 | 上海市农业科学院 | A kind of culture medium and cultural method for being used to cultivate preeminent hickory chick |
| CN109504612A (en) * | 2019-01-23 | 2019-03-22 | 上海市农业科学院 | A kind of cultural method and its culture medium of Morchella esculenta (L.) Pers mycelium |
| CN109566272A (en) * | 2019-01-23 | 2019-04-05 | 上海市农业科学院 | A kind of cultural method and its culture medium of Morchella esculenta (L.) Pers mycelium |
| CN112400607A (en) * | 2020-10-14 | 2021-02-26 | 安徽乐永园农业科技有限公司 | Preparation and use methods of culture medium suitable for culturing morchella |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823429A (en) * | 2012-09-03 | 2012-12-19 | 朱斗锡 | Novel morel cultivation method |
| CN103688745A (en) * | 2013-12-02 | 2014-04-02 | 兴化市板桥食用菌有限公司 | Pholiota adiposa cultivating method |
| CN103688745B (en) * | 2013-12-02 | 2015-10-28 | 兴化市板桥食用菌有限公司 | A kind of yellow umbrella mushroom cultivation method |
| CN107653194A (en) * | 2017-11-14 | 2018-02-02 | 上海市农业科学院 | A kind of culture medium and cultural method for being used to cultivate preeminent hickory chick |
| CN107653194B (en) * | 2017-11-14 | 2019-09-27 | 上海市农业科学院 | It is a kind of for cultivating the culture medium and cultural method of preeminent hickory chick |
| CN109504612A (en) * | 2019-01-23 | 2019-03-22 | 上海市农业科学院 | A kind of cultural method and its culture medium of Morchella esculenta (L.) Pers mycelium |
| CN109566272A (en) * | 2019-01-23 | 2019-04-05 | 上海市农业科学院 | A kind of cultural method and its culture medium of Morchella esculenta (L.) Pers mycelium |
| CN109566272B (en) * | 2019-01-23 | 2022-09-20 | 上海市农业科学院 | Method for culturing morchella mycelium and culture medium thereof |
| CN112400607A (en) * | 2020-10-14 | 2021-02-26 | 安徽乐永园农业科技有限公司 | Preparation and use methods of culture medium suitable for culturing morchella |
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Application publication date: 20110119 |