The specific embodiment
Embodiment 1:
(unit is weight percentage to take by weighing 40% Purple Perilla Seed Oil respectively; Down with), 38% soybean lecithin, 15% DHA, 5% vitamin E and 2% beeswax, soybean lecithin is crossed 80 mesh sieves, Purple Perilla Seed Oil and beeswax are heated to fusing and stirring; Put then and be chilled to room temperature; Soybean lecithin, Purple Perilla Seed Oil and beeswax mixture, DHA and the vitamin E got after sieving stir, ground 20 minutes, mixed raw material are incapsulated every 0.6g.
Embodiment 2:
Take by weighing 45% Purple Perilla Seed Oil, 32% soybean lecithin, 16% DHA, 4% vitamin E and 3% beeswax respectively; Soybean lecithin is crossed 80 mesh sieves; Purple Perilla Seed Oil and beeswax are heated to fusing and stirring, put then and be chilled to room temperature, soybean lecithin, Purple Perilla Seed Oil and beeswax mixture, DHA and the vitamin E got after sieving stir, ground 25 minutes; Mixed raw material is incapsulated every 0.6g.
Embodiment 3:
Take by weighing 50% Purple Perilla Seed Oil, 30% soybean lecithin, 10% DHA, 8% vitamin E and 2% beeswax respectively; Soybean lecithin is crossed 80 mesh sieves; Purple Perilla Seed Oil and beeswax are heated to fusing and stirring, put then and be chilled to room temperature, soybean lecithin, Purple Perilla Seed Oil and beeswax mixture, DHA and the vitamin E got after sieving stir, ground 30 minutes; Mixed raw material is incapsulated every 0.6g.
Embodiment 4:
Take by weighing 55% Purple Perilla Seed Oil, 28% soybean lecithin, 12% DHA, 2% vitamin E and 3% beeswax respectively; Soybean lecithin is crossed 80 mesh sieves; Purple Perilla Seed Oil and beeswax are heated to fusing and stirring, put then and be chilled to room temperature, soybean lecithin, Purple Perilla Seed Oil and beeswax mixture, DHA and the vitamin E got after sieving stir, ground 20 minutes; Mixed raw material is incapsulated every 0.6g.
Embodiment 5:
Take by weighing 60% Purple Perilla Seed Oil, 26% soybean lecithin, 11% DHA, 1% vitamin E and 2% beeswax respectively; Soybean lecithin is crossed 80 mesh sieves; Purple Perilla Seed Oil and beeswax are heated to fusing and stirring, put then and be chilled to room temperature, soybean lecithin, Purple Perilla Seed Oil and beeswax mixture, DHA and the vitamin E got after sieving stir, ground 25 minutes; Mixed raw material is incapsulated every 0.6g.
Embodiment 6:
The zoopery of auxiliary improving memory function of the present invention
1 material and method
1.1 sample: provided by the Wuxi City health bio tech ltd of being bestowed by heaven, the oral RD of human body is 2.4g every day, calculates with everyone 60kg body weight, amounts to dosage 0.04g/kg.bw.
1.2 animal used as test: totally 240 of SPF level male mice in kunming, body weight is 18~22g, and animal used as test production licence number SCXK (Henan) 2005-0001 is provided by Henan Province's Experimental Animal Center.Feed is provided by Changsha Kaifu District east wound animal used as test science service portion, and production licence number is SCXK (Hunan) 2006-0001.
1.3 experimental situation condition: experiment condition is shielding environment, 22~24 ℃ of experimental session experimental situation Wang Wen degree, humidity 52~58%.The animal used as test occupancy permit number is SCXK (Hunan) 2005-0001.
1.4 dose groups is selected and is tried thing and give mode: according to the oral recommended amounts of human body, establish capsule of the present invention low, in, do dosage and be respectively 0.20g/kg.bw, 0.40g/kg.bw, 1.20g/kg.bw (be equivalent to respectively human body RD 5,10,30 times).Basic, normal, high dosage receives the test solution preparation time to get capsule 4.00g of the present invention, 8.00g, 24.00g respectively to add vegetable oil to 200ml, and control group is given isopyknic solvent, irritates stomach every day once, irritates that body of stomach is long-pending to be 0.1ml/10g.bw, continuous 30 days.
1.5 key instrument: water maze, keep away dark appearance, diving tower appearance (providing) by the disease prevention and control center, Hunan Province.
1.6 experimental technique:
1.6.1 step down test: the male mice of body weight 18~22g is divided into three dose groups and a control group, 10 every group at random.The continuous irrigation stomach carries out step down test after 30 days.Animal is put into reaction chamber endoadaptation 3min, and passing to 36 volts of alternating currents immediately stimulates mouse.Then mouse is placed on the insulated platform, writes down each mouse and jump off the number of animals that every mouse is shocked by electricity in incubation period, 5min of platform number of times (jumping off the errors number of platform) and each group are jumped off platform for the first time, with this as school grade.Cyclical test behind the 24h, record are jumped off incubation period, the errors number of jumping off platform in the 3min of platform for the first time and are respectively organized the number of animals of jumping off platform.Stop to train the test of disappearing after 5 days.
1.6.2 keep away dark test: animal is selected, and test is divided into groups, and gives all same step down test of dosage, approach time that is tried thing.The continuous irrigation stomach begins to keep away dark test after 30 days.Write down every mouse and get into the errors number in darkroom and the number of animals that each group gets into the darkroom to getting into shocked by electricity required time (incubation period), 5min of darkroom from putting into bright chamber.The same time tests again behind the 24h, writes down the interior errors number in darkroom and the number of animals that each group gets into the darkroom of getting into of incubation period, 5min that every mouse gets into the darkroom.Stop to train the test of disappearing after 5 days.
1.6.3 water maze test: the male mice of body weight 18~22g is divided into three dose groups and a control group, 10 every group at random.Begun to carry out water maze test after 30 days to trying thing.Training period continues to be tried thing.Train once every day, continuous 5 days.Before the training in first day mouse is placed near the ladder, it is climbed up 3 times automatically, before each later on training mouse is placed near the ladder, it is climbed up 1 time automatically.During training in first day, retaining extremely begins training from the A point at the A place with baffle plate; Second day from B beginning, and this distance training 3 days is reached home in 2min to 80% animal; Training from the off in the 5th day; At last estimate (promptly respectively organize total time, total errors number of reaching home in 5 days and respectively organize the number of animals of reaching home in two minutes) with total school grade of 5 days.Stop to train the test of disappearing after 5 days.
1.7 experimental data statistics: the time of reaching home in the water maze test, total errors number, step down test and incubation period, the errors number of keeping away in the dark test belong to measurement data; Available variance analysis; But need carry out homogeneity test of variance earlier; If variance is neat, adopt one-way analysis of variance totally to compare, find differences again and to carry out comparing in twos between a plurality of dose groups and control group mean with the Dunnett method.If heterogeneity of variance then carries out the conversion of suitable variable to initial data, satisfy homogeneity test of variance after, add up with the data after the conversion; If do not reach the neat purpose of variance yet after the variable conversion, to use rank test instead and add up, discovery is overall more variant, then adopts Tamhane ' the sT2 check that does not require homogeneity of variance to compare in twos.Jump off in the number of animals of reaching home in the 2min in the water maze test (percentage), the step down test platform number of animals (percentage), to keep away in the dark test number of animals (percentage) that gets into the darkroom be enumeration data, uses X
2Check, the total routine number of four fold table is less than 40, or total routine number is equal to or greater than 40 but theoretical value occurs and be equal to or less than at 1 o'clock, uses definite probabilistic method instead.
1.8 the result judges: step down test, keep away and appoint the binomial trials result positive in three tests of dark test water maze test; And repeated test result's consistent (same the test two times result that is repeated is all positive) can judge that this given the test agent is that auxiliary improving memory function animal test results of the present invention is positive.
2 result of the tests for the first time
2.1 the present invention is to the influence of mouse body weight
Table 1 the present invention is to the influence (water maze test)
of mouse body weight
Table 2 the present invention is to the influence (keeping away dark test)
of mouse body weight
Table 3 the present invention is to the influence (step down test)
of mouse body weight
1-3 is visible by table, and per os continuous irrigation stomach gives the content of the present invention 30 days of mouse various dose, each dose groups mouse body weight and control group comparison, and there are no significant for difference (P>0.05).
2.2 the present invention is to the influence of mouse water maze test
Table 4 the present invention is to the reach home influence
of time of mouse in the water maze test
Visible by table 4, time that each dose groups mouse is reached home and control group comparing difference do not have conspicuousness (P>0.05).
Table 5 the present invention is to the reach home influence
of completely wrong mistake number of times of mouse in the water maze test
Visible by table 5, errors number and control group comparing difference before the high dose group mouse is reached home have conspicuousness (P<0.05).
Table 6 the present invention is to the influence of the number of animals of reaching home in the 2min in the water maze test
Visible by table 6, number of animals that each dose groups mouse is reached home and control group comparing difference do not have conspicuousness (P>0.05).
2.3 the present invention keeps away the influence of dark test to mouse
Table 7 the present invention keeps away the preclinical influence of dark test
to mouse
Visible by table 7, the incubation period and the control group that get into the darkroom during each dose groups mouse training compare difference that there are no significant (P>0.05).High dose group mouse test incubation period and control group compare, and difference has conspicuousness (P<0.05).
Table 8 the present invention keeps away the influence
of dark experimental mistake number of times to mouse
Visible by table 8, each dose groups training errors number and control group compare, and difference does not have conspicuousness (P>0.05).Each dose groups test errors number and control group compare, and difference does not have conspicuousness (P>0.05).
Table 9 the present invention keeps away the influence of dark test test wrong reaction rate to mouse
Visible by table 9, number of animals and control group that each dose groups gets into the darkroom compare, and difference does not have conspicuousness (P>0.05).
2.4 the present invention is to the influence of mouse step down test
Table 10 the present invention is to the preclinical influence of mouse step down test
Visible by table 10, the incubation period and control group comparison of jumping off platform during each dose groups mouse training and during test, difference does not have conspicuousness (P>0.05).
Table 11 the present invention is to the influence
of mouse step down test errors number
Visible by table 11, each dose groups mouse training errors number and test errors number control group compare, and difference does not have conspicuousness (P>0.05).
Table 12 the present invention is to the influence of mouse diving tower test wrong reaction rate
Visible by table 12, the number of animals and the control group that jump off platform during each dose groups mouse test compare, and difference does not have conspicuousness (P>0.05).
2.5 the influence that the present invention is disappeared and tested the mouse water maze
Table 13 the present invention is to the influence of mouse memory represents in the water maze test---the test of disappearing
Visible by table 13, water maze disappear in the test each dose groups mouse reach home time, the errors number of reaching home and reach home in 2 minutes number of animals and control group comparison, difference does not have conspicuousness (P>0.05).
2.6 the present invention keeps away the influence of the test of secretly disappearing to mouse
Table 14 the present invention is to keeping away the influence of mouse memory represents in the dark test---the test of disappearing
Visible by table 14, keeping away the errors number that each dose groups mouse in the test of secretly disappearing gets into the darkroom and comparing with number of animals that gets into the darkroom and control group, there are no significant for difference (P>0.05).Incubation period and control group that the high dose group mouse gets into the darkroom compare, and difference has conspicuousness (P<0.05).
2.7 the influence that the present invention is disappeared and tested the mouse diving tower
Table 15 the present invention is to the influence of mouse memory represents in the step down test---the test of disappearing
Visible by table 15, each dose groups mouse jumps off incubation period, the errors number of platform, the number of animals of jumping off platform and control group comparing difference does not have conspicuousness (P>0.05).
3 result of the tests for the second time
Table 16 the present invention is to the influence (water maze test)
of mouse body weight
Table 17 the present invention is to the influence (keeping away dark test)
of mouse body weight
Table 18 the present invention is to the influence (step down test)
of mouse body weight
16-18 is visible by table, and per os continuous irrigation stomach gives the content of the present invention 30 days of mouse various dose, each dose groups mouse body weight and control group comparison, and there are no significant for difference (P>0.05).
3.2 the present invention is to the influence of mouse water maze test
Table 19 the present invention is to the reach home influence
of time of mouse in the water maze test
Visible by table 19, time that each dose groups mouse is reached home and control group comparing difference do not have conspicuousness (P>0.05).
Table 20 the present invention is to the reach home influence
of completely wrong mistake number of times of mouse in the water maze test
Visible by table 20, errors number and control group comparing difference before the high dose group mouse is reached home have conspicuousness (P<0.05).
Table 21 the present invention is to the influence of the number of animals of reaching home in the 2min in the water maze test
Visible by table 21, number of animals of reaching home in the high dose group mouse 2min and control group comparing difference have conspicuousness (P<0.05).
3.3 the present invention keeps away the influence of dark test to mouse
Table 22 the present invention keeps away the preclinical influence of dark test
to mouse
Visible by table 22, the incubation period and the control group that get into the darkroom during each dose groups mouse training compare difference that there are no significant (P>0.05).High dose group mouse test incubation period and control group compare, and difference has conspicuousness (P<0.05).
Table 23 the present invention keeps away the influence
of dark experimental mistake number of times to mouse
Visible by table 23, each dose groups training errors number and control group compare, and difference does not have conspicuousness (P>0.05).Each dose groups test errors number and control group compare, and difference does not have conspicuousness (P>0.05).
Table 24 the present invention keeps away the influence of dark test test wrong reaction rate to mouse
Visible by table 24, number of animals and control group that each dose groups gets into the darkroom compare, and difference does not have conspicuousness (P>0.05).
3.4 the present invention is to the influence of mouse step down test
Table 25 the present invention is to the preclinical influence of mouse step down test
Visible by table 25, the incubation period and control group comparison of jumping off platform during each dose groups mouse training and during test, difference does not have conspicuousness (P>0.05).
Table 26 the present invention is to the influence
of mouse step down test errors number
Visible by table 26, each dose groups mouse training errors number and test errors number control group compare, and difference does not have conspicuousness (P>0.05).
Table 27 the present invention is to the influence of mouse diving tower test wrong reaction rate
Visible by table 27, the number of animals and the control group that jump off platform during each dose groups mouse test compare, and difference does not have conspicuousness (P>0.05).
3.5 the influence that the present invention is disappeared and tested the mouse water maze
Table 28 the present invention is to the influence of mouse memory represents in the water maze test---the test of disappearing
Visible by table 28, water maze disappear in the test each dose groups mouse reach home time, the errors number of reaching home and reach home in 2 minutes number of animals and control group comparison, difference does not have conspicuousness (P>0.05).
3.6 the present invention keeps away the influence of the test of secretly disappearing to mouse
Table 29 the present invention is to keeping away the influence of mouse memory represents in the dark test---the test of disappearing
Visible by table 29, number of animals and the control group of keeping away incubation period, errors number and the entering darkroom in each dose groups mouse entering darkroom in the test of secretly disappearing compare, and there are no significant for difference (P>0.05).
3.7 the influence that the present invention is disappeared and tested the mouse diving tower
Table 30 the present invention is to the influence of mouse memory represents in the step down test---the test of disappearing
Visible by table 30, each dose groups mouse jumps off incubation period, the errors number of platform, the number of animals of jumping off platform and control group comparing difference does not have conspicuousness (P>0.05).
4 results
Carry out memory test after 30 days for continuously mouse stomach with the content of the present invention of 0.20g/kg.bw, 0.40g/kg.bw, 1.20g/kg.bw dosage.
Result of the test shows for the first time: in the water maze test, the errors number that 1.20g/kg.bw dose groups mouse is reached home is less than control group, and difference has conspicuousness (P<0.05); Keep away in the dark test, 1.20g/kg.bw dose groups mouse test incubation period and control group compare, and difference has conspicuousness (P<0.05); In the step down test, each dose groups mouse each item test index and control group compare, and difference does not have conspicuousness (P>0.05).Incubation period and the control group of keeping away 1.20g/kg.bw dose groups mouse entering darkroom in the test of secretly disappearing compare, and difference has conspicuousness (P<0.05); Water maze disappear test and diving tower disappear each dose groups mouse each item test index and control group comparison in the test, difference does not have conspicuousness (P>0.05).
Result of the test shows for the second time: in the water maze test, the errors number that 1.20g/kg.bw dose groups mouse is reached home is less than control group, and the number of animals of reaching home in the 2min is more than control group, and difference has conspicuousness (P<0.05); Keep away in the dark test, 1.20g/kg.bw dose groups mouse test incubation period and control group compare, and difference has conspicuousness (P<0.05); In the step down test, each dose groups mouse each item test index and control group compare, and difference does not have conspicuousness (P>0.05).Water maze disappear test, keep away secretly disappear test and diving tower disappear each dose groups mouse each item test index and control group comparison in the test, difference does not have conspicuousness (P>0.05).
5 sum up
Under this laboratory condition, give mouse stomach 30 days continuously with the content of the present invention of 0.20g/kg.bw, 0.40g/kg.bw, 1.20g/kg.bw dosage, twice experimental result show, water maze, to keep away dark test front and back two times result all positive.Judge that according to " health food check and assessment technique standard " (2003 editions) the present invention has auxiliary improving memory function to animal subject.
Embodiment 7:
Auxiliary improving memory function human experiment experiment of the present invention
1 material and method
1.1 sample: capsule No. 1, No. 2 provides by the Wuxi City health bio tech ltd of being bestowed by heaven.The two is basically identical on packing, outward appearance, color and luster and mouthfeel, and one of them is a content of the present invention, and another is a placebo.Oral RD every day 2 times, each 2.
1.2 the experimenter selects
1.2.1 the standard of including in: the experimenter is from Hengyang City unit, 18~65 years old age, male or female.Physical condition is good; Do not have obvious brain, the heart, liver, kidney, hematologic disease, do not have the history of taking medicine for a long time, do not accept similarly test (memory quotient, IQ test); Do not take medicine or the health food relevant with improving memory in 1 year, the volunteer guarantees to cooperate.
1.2.2 exclusion standard: gestation or women breast-feeding their children, to the health food allergy sufferers; Merge to have the inclination, serious disease patients such as liver, kidney and hemopoietic system; Take the article relevant in a short time, have influence on judgement person as a result with being tried function; Do not meet the standard of including in,, can't judge effect or data not umbra sound effect or security judgement person not by the practical given the test agent of regulation.
1.3 experimental design and grouping
Adopt contrast, double blinding, method for designing at random.Carry out the test first time before the clothes appearance; Be divided into test-meal group and control group at random by memory quotient; Consider to influence result's the principal element such as the harmony at cultural level, age etc. as far as possible, with comparativity between the assurance group, after two groups of memory quotient equilibriums of check; Randomly drawing one group again is test group, and another group is control group.
1.4 experimental technique
Test-meal group and control group began from November 22nd, 2009, took sample by RD.Take by responsible sample of special messenger and supervision.Duration of test is not taken and is improved memory relevant health care food or medicine, does not participate in memory quotient or the IQ test irrelevant with this test.Oxygen enrichment carries out the test second time after 45 days continuously.
2 effect indexs
Use the test of Clinical Memory scale, look into scale score with the original branch of each subtest after the test: sensing memory, associative learning, image are freed recall, random shape is re-recognized, the contact of portrait characteristics is recalled.Each subtest scale score addition gets total scale score, looks into memory quotient with total scale score.
2.1 test equipment: record has the memory of sensing and the instruction of associative learning and the tape of stimulus; Picture material, the image image picture of freeing recall, two groups each 15, totally 30 (dividing first, second two covers); Random shape is re-recognized picture, is equipped with to appear with 20 in goal stimulus picture for the first time, and being equipped with to appear when re-recognizing for the second time with goal stimulus stimulates each 20 in picture with sneaking into, and totally 40, adds up to 60 pictures (branch first, second two covers); Picture is recalled in the contact of portrait characteristics, is used black and white for the first time fully and delineates people's face as 6, and the people's face that appears when recalling fully is as 6, and both contents are identical, and order is different, amounts to 12; Every portrait picture back side indicates characteristics (branch first, second two covers) such as the surname, occupation, hobby of this portrait.Recorder, stopwatch, record-paper.
2.2 method of testing and requirement
2.2.1 method of testing: forward and backward twice test of each experimenter reduces systematic error by same main examiner's testing.The test time point: forward and backward twice test of each experimenter carried out at same time point, avoids the influence of biological rhythm.
When testing for the second time, the main examiner does not see the grouping list, blind method test.
2.2.2: the general requirement during test: in a quiet room, the experimenter is tested by the personnel that received specialized training; Except that experimenter and main examiner, avoided other people on the scene as far as possible; Three subtests relevant with vision are arranged in this scale, and indoor light must guarantee to see clearly Chu stimulates picture; Get rid of because of hearing or dysphotia as far as possible and influence the memory achievement; Must be noted that the state of mind the when experimenter is tested, carry out under the situation that test needs normally, not oppose to accept to test in experimenter's mood, notice is relatively concentrated; Whether whether whether the experimenter is tired, and whether notice is concentrated, cooperate, nervous to test, and whether confident the grade all need be recorded on the homepage of record-paper; Same experimenter's test request is once finished; When comparing the subtest achievement, must be noted that what whether different subtests carried out under the identical state of mind with the age scale branch.Errorless can the inserting in the original subitem of original branch check of each item subtest achievement.
3 data statistics
This test data is a measurement data, can analyze with the t check two groups of each subtest scale scores and memory quotient.Own control can adopt paired t-test; The parallel t check of relatively adopting two sample copy means between group, the latter need carry out homogeneity test of variance, and the data of Non-Gaussian Distribution or heterogeneity of variance are carried out suitable variable conversion; Wait to satisfy normal state or variance neat after, carry out the t check with data converted; If translation data still can not satisfy the neat requirement of normal state variance, use t ' check or rank test instead; But the coefficient of variation too data of big (like CV>50%) is used rank test.Add up with INSTAT, SPSS statistical software.
4 results judge
Under the prerequisite that two groups of memory quotient are balanced before test; The memory quotient of test-meal group is higher than control group after the test-meal, and difference has conspicuousness, and the memory quotient after the test of the group of test-meal simultaneously is higher than the memory quotient before its test; And difference has conspicuousness, can judge that this given the test agent has the effect that improves memory function.
5 the present invention are to the influence of human body memory effect index
Table 31 the present invention is to pointing to the influence
of memory scale score
Compare with control group, #P<0.05 is compared with self in * P<0.05
Visible by table 31, the sensing memory scale score of test group compares with control group before the clothes appearance, and difference does not have conspicuousness (P>0.05); Relatively, difference all has conspicuousness (P<0.05) before the sensing memory scale score of clothes appearance back test group and control group and self the clothes appearance.
Table 32 the present invention is to the influence
of associative learning scale score
Compare with control group, #P<0.05 is compared with self in * P<0.05
Visible by table 32, the associative learning scale score of test group and control group compare before the clothes appearance, and difference does not have conspicuousness (P>0.05); Relatively, difference all has conspicuousness (P<0.05) before the associative learning scale score of clothes appearance back test group and control group and self the clothes appearance.
Table 33 the present invention is to the free recall influence
of scale score of image
Compare with control group, #P<0.05 is compared with self in * P<0.05
Visible by table 33, the image of the test group scale score of freeing recall compares with control group before the clothes appearance, and difference does not have conspicuousness (P>0.05); The image of clothes kind back test group is freed recall and is compared before scale score and control group and self are obeyed appearance, and difference all has conspicuousness (P<0.05).
Table 34 the present invention re-recognizes the influence
of scale score to random shape
Visible by table 34, the random shape of the preceding test group of clothes appearance re-recognizes scale score and control group compares, and difference does not have conspicuousness (P>0.05); The random shape of clothes kind back test group compares before re-recognizing scale score and control group and self clothes appearance, and difference does not have conspicuousness (P>0.05).
Table 35 the present invention is to the influence
of portrait characteristics contact memory scale score
Compare with control group, #P<0.05 is compared with self in * P<0.05
Visible by table 35, the portrait characteristics contact of test group is recalled scale score and is compared with control group before the clothes appearance, and difference does not have conspicuousness (P>0.05); Relatively, difference all has conspicuousness (P<0.05) before the portrait characteristics contact memory scale score of clothes appearance back test group and control group and self the clothes appearance.
Table 36 the present invention is to the influence
of memory quotient
Compare with control group, #P<0.05 is compared with self in * P<0.05
Visible by table 36, the memory quotient of test group and control group compare before the clothes appearance, and difference does not have conspicuousness (P>0.05); The memory quotient of clothes appearance back test group is significantly higher than control group (P<0.05); Memory quotient after the test group clothes appearance is significantly higher than clothes appearance preceding (P<0.05).
6 take off the mistake rate
After experiment in 45 days, control group has 3 routine experimenters to receive test product or can't be screened out by determine effect because of being interrupted to take; The test-meal group has 2 routine experimenters to receive test product or can't be screened out by determine effect because of being interrupted to take.Last efficiency test crowd control group 51 examples, test-meal group 52 examples.See table 37.
The mistake rate is taken off in table 37 test
7 sum up
Human feeding trial is the result show; The experimenter takes content of the present invention after 45 days continuously; Test group sensing memory, associative learning, image are freed recall, the portrait characteristics relatively are improved before getting in touch and recalling scale score and memory quotient and control group and self clothes appearance, and difference has conspicuousness (P<0.05).According to " health food check and assessment technique standard " (version in 2003) evaluation criterion, the effect that prompting the present invention has auxiliary improvement human body memory function.