CN101940241A - Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof - Google Patents
Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof Download PDFInfo
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- CN101940241A CN101940241A CN 201010225899 CN201010225899A CN101940241A CN 101940241 A CN101940241 A CN 101940241A CN 201010225899 CN201010225899 CN 201010225899 CN 201010225899 A CN201010225899 A CN 201010225899A CN 101940241 A CN101940241 A CN 101940241A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
- C11B1/08—Production of fats or fatty oils from raw materials by pressing by hot pressing
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
- C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
- C11C3/10—Ester interchange
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Abstract
The invention provides pangasianodon gigas lipid, application and a method for preparing breast milk lipid substitutes thereof. The lipid from the pangasianodon gigas is catfish fat or a fractionation liquid component thereof extracted from whole fish of the pangasianodon gigas or other positions after fillets are removed. The pangasianodon gigas lipid is used for preparing the breast milk lipid substitutes. The palmitic acid content on the Sn-2 position in the breast milk lipid substitutes prepared by the pangasianodon gigas lipid is relatively high, which is accordant to that on the Sn-2 position in the breast milk lipid, namely reaches over 60 percent. Meanwhile, AA and DHA exist in the pangasianodon gigas fat or the fractionation liquid component thereof, so the breast milk lipid substitutes does not need the extra AA and DHA or only needs to add a small quantity of the AA or DHA .
Description
Technical field
The invention belongs to the edible oil and fat field, relate in particular to a kind of grease and application in the human milk fat substituted thing of preparation thereof that derives from the Mekong Delta catfish.
Background technology
Breast milk is the most perfect food that is fit to infants growth and development.Therefore WHO advocated behind baby due breast feeding at least 6 months, to guarantee infants growth and development, to improve immunity and infection prevention disease.Contain 4～4.5% the fat of having an appointment in the breast milk, wherein 98% is triglycerides.The kind complexity of aliphatic acid in the breast milk, saturated fatty acid comprise, medium chain, middle long-chain and chain saturated fatty acids are as laurate (5-7%), palmitic acid (20-24%), stearic acid (7.1-9%); Monounsaturated fatty acids comprises that oleic acid (31-38%), palmitoleic acid (2.5-3.8%), n-3 polyunsaturated fatty acid are except that containing linoleic acid; The n-3 polyunsaturated fatty acid except that containing alpha-linolenic acid, also contain DHA (DHA, 0.3-1.9%).
Fat in the initial babies ' formula milk powder mainly is the cow's milk fat in the whole milk powder, but no matter is that aliphatic acid is formed or lipid structure in the cow's milk fat, all with human milk fat significant difference is arranged.The saturated fatty acid of low carbon chain (C4-C10) content is higher in the cow's milk fat, and polyunsaturated fatty acid such as C18:2 content are lower, and other polyunsaturated fatty acid C18:3, C20:4 and C22:6 etc. almost do not have.In addition, the saturated fatty acid in the cow's milk mainly concentrates on Sn-1, and on 3, the saturated fatty acid in the breast milk (mainly being C16:0) is then most of on the Sn-2 position.
As previously mentioned, saturated aliphatic acid (SFA) content significantly is lower than cow's milk fat in the human milk fat, and unrighted acid (TUFA) and polyunsaturated fatty acid (PUFA) content are significantly higher than cow's milk fat.In infant formula, can eliminate these differences by adding vegetable oil.At present, at home in the babies ' formula milk powder contained oil source mainly contain: 1. use the one-level refining vegetable oil of single variety to mix with cow's milk is fatty; 2. the multiple refining vegetable oil of Shi Yonging mixes with cow's milk fat; 3. all adopt vegetable fat, palmitic acid is wherein provided by palm oil.The vegetable oil kind that relates to has: high oleic sunflower oil, Canola Oil, soybean oil, palm oil, palm kernel oil, coconut oil, sunflower oil, the multiple oil plant of safflower wet goods.
Many patents have been arranged in the world, as US5,601,860 etc., be the method that directly is in harmonious proportion with vegetable oil.Main with the oil that contains essential fatty acid (as soybean wet goods) the mediation gained of the oil (as the palm wet goods) that is rich in C16:0 with other.Directly replace people's butterfat with milk, as previously mentioned, wherein sweet three ester aliphatic acid and people's butterfat have difference.As being equipped with vegetable oil again, also can make sweet three ester structures be difficult to meet the requirements.With the vegetable oil is raw material, carries out ester exchange at random with chemical catalyst such as sodium methoxide, as patent EP0376628.
Mixing by the several plant grease can obtain the product that aliphatic acid is formed and human milk fat is close, but (sweet three ester types are mainly the USU type because human milk fat has special lipid structure, and palmitic acid mainly is present on the Sn-2 position), so be that the mixed vegetable oil of palmitic acid main source can only reach aliphatic acid and forms with human milk fat approachingly with the palm oil, but can not reach the level that is close with the human milk fat structure.This compound lard is hydrolyzed into free fatty and monoglyceride through pancreatic lipase.And enteral free palmitic acid is easy and metal ion forms insoluble calcium soap, thereby causes the dual loss of calcium ion and energy.Recent two decades comes, some nutrition and clinic study person have contrasted the grease of this two class formation, and (palmitic acid mainly is positioned at Sn-2 position and Sn-1,3 positions) influence that infant nutrition is absorbed finds that it is the mixed plant grease in main palmitic acid source that grease that palmitic acid mainly is positioned at the Sn-2 position is better than with palm oil.Promptly with the similar palmitic acid status of a sovereign of human milk fat structure in the grease of Sn-2 position, help improving fat absorptivity, strengthen calcium absorption, improve bone density and reduce the infant firmly just, prevent constipation.
As far back as going deep into of research that the eighties absorbs along with infant nutrition in last century, the preparation research that mainly is positioned at the structure grease of Sn-2 position about palmitic acid launches.The key for preparing human milk fat substituted thing is to locate the grease of the high palmitic acid content in Sn-2 position.The high palmitic acid content glyceride stock in high Sn-2 position of report has two classes at present: the palm oil of palmitic stearin and ester exchange.Lard also is the higher grease of a kind of Sn-2 palmitic acid content, and the ratio of palmitic acid was 20-30% during aliphatic acid was formed, and the palmitic acid content on the Sn-2 position is then up to more than 60%.But there is religious taboo in lard as edible oil, can not be widely used as glyceride stock.Natural distributed is on Sn-1 or Sn-3 position for saturated aliphatic acid in the vegetable oil (mainly being stearic acid and palmitic acid), and the palmitic acid content on the Sn-2 position seldom.The palmitic acid ratio was up to more than 40% during palmitic aliphatic acid was formed, but most palmitic acid is distributed on Sn-1 or the Sn-3 position, and the palmitic acid content on the Sn-2 position is less than 10%.
In order to obtain the vegetable fat of the high palmitic acid content in Sn-2 position, can adopt the method for oil modification: ester exchange and branch are carried.(1) ester exchange: with palm oil under the effect of chemical catalyst (sodium methoxide or caustic alcohol), carry out ester exchange reaction at random, palmitic acid is evenly distributed on Sn-1, the Sn-3 and Sn-3 position of glyceride, thus make on the Sn-2 position content of palmitic acid reach total aliphatic acid form in the level of palmitic acid more than 40%.(2) the grease branch is carried: utilize the fusing point difference of palmitic various sweet three esters of formation and the difference of solubility at a certain temperature, palm oil is divided into solid, liquid two parts, make the content of the PPP (tripalmitin) in the solid portion improve, thereby the palmitic acid content on the Sn-2 position is brought up to the expection level.But the palmitic acid content in these two kinds of resulting glyceride stocks of method on the Sn-2 position can not surpass 33.3%.
According to the grease of the above-mentioned high palmitic acid content in Sn-2 position and the aliphatic acid difference between the breast milk, select the fatty acid mixed or the aliphatic acid low-carbon-ester mixture of rational proportion, at Sn-1, carry out ester exchange reaction under the catalysis of 3 specific lipases, reactant deodorization under vacuum condition, remove fatty acid mixed or aliphatic acid low-carbon-ester mixture unnecessary and that reaction cements out, can obtain the grease approaching with the human milk fat structure.But because the limitation of the palmitic acid content on the Sn-2 position in the raw material, adopt the palmitic acid content on the Sn-2 position in the final products that this method obtains also not high.
The preparation of present human milk fat substituted thing mainly contains two kinds of methods: ester exchange reaction and grease and fatty acid mixed the ester exchange reaction lipase-catalyzed under of compound lard under lipase-catalyzed.
The ester exchange reaction preparation of compound lard under lipase-catalyzed: report among the WO1994/268551 of Unilever house journal, with a certain proportion of palm oil and palm-kernel oil 1, carry out ester exchange under 3 selected fat enzymatics, ester exchange offspring and high oleic sunflower oil, sunflower oil and coconut oil are allocated by a certain percentage, obtain saturated fatty acid and account for 30% sweet three ester admixtures, wherein the saturated fatty acid on the Sn-2 position accounts for more than 40% of total saturated fatty acid, and is approaching with human milk fat.
Grease and fatty acid mixed prepare in the ester exchange reaction under lipase-catalyzed:
In the U.S. Pat 4876107 palm oil high-melting-point branch is put forward component (composition is 80%PPP and 20%POP) and mix at 3: 1 with weight ratio, be dissolved in the normal hexane, issue the rearrangement reaction of oil generation fat in the lipase effect that derives from Mucor under 40 ℃ with oleic acid.Filter and remove enzyme, remove normal hexane by distillation again, free fatty is removed in deodorization under the vacuum condition, and product is allocated with butterfat, sunflower oil, soybean oil and coconut wet goods again, obtains human milk fat substituted thing.
In the U.S. Pat 5658768 palmitic stearin is mixed by weight 1: 0.75 with high oleic sunflower oil aliphatic acid, in the lipase-catalyzed reaction down of immobilization Novo SP-392, remove unnecessary aliphatic acid by distillation again, product under two sweet ester specific lipase Amanolipase G effects with fatty acid response, reduce the content of two sweet esters in the product.50% this product mixes back ester exchange reaction under lipase Novo SP-392 catalysis with 30% liquid vegetable oil, 20 coconut oil, reduce the content of three saturated glyceride in the grease, thereby obtain human milk fat substituted thing.
Sweet three esters with the high palmitic acid content in Sn-2 position (〉=90%) in the European patent EP 0496456 mix with weight ratio with canola oil aliphatic acid at 1: 1, and by the packed column reaction of immobilized lipase NovoSP-392 is housed, 70 ℃ of reaction temperatures are not added solvent.Reaction finishes, and 93% aliphatic acid is transferred in sweet three esters, has produced 5% sweet diester simultaneously.Unnecessary aliphatic acid is removed by distillation, and removes the free fatty of trace through neutralization.Sweet diester is then through unmodified packed column absorption removal.Above-mentioned product is dissolved in the acetone again, and the solvent branch is carried under-5 ℃ of low temperature, is human milk fat substituted thing after the liquid part refining that obtains.
In addition, similar with above-mentioned patent, also have sweet three esters of high-purity three palmitic acids (PPP) and oleic acid or methyl oleate at the lipase-catalyzed report that carries out acidolysis reaction down.
But above-mentioned preparation method has various defectives, account for total palmitic acid as the palmitic acid on the human milk fat substituted thing Sn-2 position of the ester exchange reaction preparation of compound lard under lipase-catalyzed and can only reach about 40%, account for total palmitic acid with palmitic acid on the Sn-2 position of human milk fat reality and reach far apart more than 67%.And adopt grease and the fatty acid mixed ester exchange reaction under lipase-catalyzed, because raw material mainly adopts palm oil, the ratio that palmitic acid in the palm oil on the Sn-2 position accounts for total palmitic acid lower (not being higher than 33.3%), the palmitic acid content on the Sn-2 position after the enzyme process ester exchange in the end-product is not high yet.
Also have report use China's lard raw material (sweet three esters are mainly the USU type) cheap and easy to get Lipozyme IM lipase-catalyzed down and soya fatty acid carry out acidolysis reaction, enzyme addition 13.6%, the mol ratio of lard and aliphatic acid is 1: 2.4,61 ℃ of reaction temperatures can obtain human milk fat substituted product with low cost.But there is religious taboo in lard as edible oil, can not be widely used as glyceride stock.
Summary of the invention
The objective of the invention is to the above problem of existing at existing human milk fat substituted thing that is used for infant formula and preparation method thereof, provide a kind of can be used for preparing with human milk fat form the grease that derives from the Mekong Delta catfish of approaching human milk fat substituted thing and this grease be applied to prepare human milk fat substituted thing.
The grease that derives from the Mekong Delta catfish of the present invention is extraction or the catfish fat that extracts from all the other later positions of removal fillet or the branch extract body component of described catfish fat from the full fish of Mekong Delta catfish (Pangasianodon gigas).
Described Mekong Delta catfish (Pangasianodon gigas) is meant Ba Sha catfish (basa catfish) and short, bristly hair or beard catfish (tra catfish).Can adopt wherein one or both.
As preferred version, the extracting method of described catfish fat is: Mekong Delta catfish raw material is cleaned, full fish pulverized maybe will remove raw material remaining behind the fillet and pulverize, pass through continuously cooking then, press filtration is used Steam Heating 20-60 minute with filtrate, centrifugal then, the crude fat that obtains dewaters, and refining then promptly gets catfish fat.
As preferred version, the branch extracting method of the branch extract body component of described catfish fat is: Mekong Delta catfish fat is heated to 60 ℃, after stablizing 20 minutes, be cooled to 30 ℃ with 10 ℃/hour speed, be cooled to 15 ℃, thermostatical crystallization 3-5 hour with 1.5-2.5 ℃/hour speed again, aforementioned all processes add band and stir, after solid crystal to be separated out partly reaches the 10-50% of gross weight, under 15 ℃ of temperature, begin to carry out isolated by filtration, after filtering, obtain branch extract body component.
A kind ofly adopt the method that derives from the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish of the present invention, comprise the steps:
(1) extracts the fat that derives from Mekong Delta catfish (Pangasianodon gigas);
(2) the catfish fat that previous step is obtained suddenly carries out the grease branch and carries and obtain its minute extract body component under 10-30 ℃ of temperature conditions;
(3) grease that step (1) or step (2) are obtained lipase-catalyzed down and fatty acid mixed or fatty acid mixed low-carbon-ester carry out the enzyme process ester exchange reaction, or with the grease that step (1) or step (2) obtain mix with other vegetable oil resulting compound lard lipase-catalyzed down and fatty acid mixed or fatty acid mixed low-carbon-ester carry out the enzyme process ester exchange reaction, the weight ratio of described grease or compound lard and fatty acid mixed or fatty acid mixed low-carbon-ester is (2: 1)-(1: 2), ester exchange reaction promptly gets human milk fat substituted thing after resulting grease of reaction and refining are isolated in decompression distillation.
The extracting method in described (1) step is, Mekong Delta catfish raw material is cleaned, full fish pulverized maybe will remove raw material remaining behind the fillet and pulverize, through advancing continuously cooking, press filtration is used Steam Heating 20-60 minute with filtrate then, centrifugal then, the crude fat that obtains dewaters, and refining then promptly gets catfish fat.
The temperature that described (2) step grease branch is carried is preferably 15-25 ℃.
Described aliphatic acid low-carbon-ester is preferably fatty-acid ethyl ester.
Fatty acid mixed in described (3) step derives from the hydrolysate of any compound lard of soybean oil, Canola Oil, sunflower oil, corn oil, coconut oil, palm-kernel oil; Described fatty acid mixed low-carbon-ester derives from the alcoholysis product of any compound lard of soybean oil, Canola Oil, sunflower oil, corn oil, coconut oil, palm-kernel oil.
Described lipase is Sn-1,3 specific lipases, preferred LIPOZYME RLIM.
In the described enzyme process ester exchange reaction, directly in reactant, add lipase and carry out intermittent reaction, or reactant is undertaken by the enzyme packed reaction column.
Technique effect of the present invention is: the present invention by fatty acid mixed or fatty acid mixed low-carbon-ester under 1,3 specific lipase catalysis and Mekong Delta catfish fatty or its minute sweet three ester admixtures that obtain of extract body component reaction.Because the palmitic acid content on Mekong Delta catfish fat or its minute extract body component S n-2 position is higher relatively, palmitic acid content on the Sn-2 position in the reacting final product is also higher relatively, can reach with human milk fat on the Sn-2 position palmitic acid content consistent, promptly reach more than 60%.Simultaneously since Mekong Delta catfish fat or its minute extract body component have AA and DHA, this human milk fat substituted thing does not need additionally to add AA and DHA or only need add a spot of AA or DHA.
The specific embodiment
Embodiment one:
1, extract Mekong Delta catfish fat:
Mekong Delta Ba Sha catfish (basa catfish) and short, bristly hair or beard catfish (tracatfish) are cleaned, remove fillet then, the raw material of remainder is pulverized, through carry out continuously cooking in continuous cooking machine, Steam Heating 20-60 minute used in press filtration after the slagging-off then, then that the crude fat that obtains is centrifugal, through the dehydration of flash distillation operation, final purification obtains Mekong Delta catfish fat finished product again.
Mature technology is adopted in refining described in the embodiment of the invention, and concrete steps comprise: come unstuck, depickling, decolouring and deodorization.The deodorization stage wherein is by carrying out under 240 ℃ of temperature, 65 minutes time of staying, the vacuum 2.3mbar condition.
2, the grease branch is carried:
The Mekong Delta catfish fat that above-mentioned steps is obtained is heated to 55-60 ℃, after stablizing 20 minutes, be cooled to 30 ℃ with 10 ℃/hour speed, be cooled to 15 ℃ with 1.5-2.5 ℃/hour speed again, thermostatical crystallization 3-5 hour, aforementioned all processes added band and stir, after solid crystal to be separated out partly reaches the 10-50% of gross weight, begin to carry out isolated by filtration under 15 ℃ of temperature, initial filter pressure is 3bar, is forced into 12bar and stops.After filtering, obtain branch extract body component.
3, preparation mixing-in fat acetoacetic ester:
Sunflower oil, perilla herb oil and coconut oil are mixed by 6: 2: 2 weight ratio, heat to 100 ℃, stir simultaneously, kept 20 minutes, then dehydration.1% heavy catalyst KOH joins in the ethanol with dehydration back oil, and the mol ratio of ethanol and grease is 6: 1, stirring and dissolving.Treat that grease is cooled to 60 ℃, add the above-mentioned ethanolic solution that contains catalyst, begin reaction, 3 hours time.Adding concentration in reaction back is 8% citric acid solution washing, and the mol ratio of citric acid and KOH is 1: 1, again washing for the second time.60 ℃ following vacuum suction 1-2 hour.Get end-product mixing-in fat acetoacetic ester.
4, enzyme process ester exchange:
With the Mekong Delta catfish mixing-in fat acetoacetic ester that divides extract body component and above-mentioned the 3rd step to obtain of above-mentioned the 2nd step gained is to mix at 1: 1 by weight, with mixture by being filled with the reaction column of lipase LIPOZYME RMIM, 55 ℃ of reaction temperatures, flow velocity be per hour by with the reactant of enzyme equivalent weight.Product is again at 120-150 ℃ of following molecular clock, and refining obtains human milk fat substituted thing.
Lipase LIPOZYME RMIM is available from the Novozymes Company of Denmark.
Embodiment two:
1, the extraction of Mekong Delta catfish fat:
Mekong Delta Ba Sha catfish (basa catfish) and short, bristly hair or beard catfish (tracatfish) are cleaned, through continuously cooking, press filtration, Steam Heating 20-60 minute used in the slagging-off back, then that the crude fat that obtains is centrifugal, through the dehydration of flash distillation operation, final purification obtains Mekong Delta catfish fat finished product again.
2, the preparation of fatty acid mixed:
Low erucic acid rape oil, soybean oil and coconut oil are mixed by 6: 2: 2 weight ratio, heat to 100 ℃, stir simultaneously, keep 20min, dehydration.Add alkali lye earlier and carry out saponification, isolate glycerine, add hydrochloric acid again and carry out acidifying, obtain fatty acid mixed.
3, enzyme process ester exchange:
Mekong Delta catfish fat is 1: 1 with the weight ratio of fatty acid mixed, and the addition of lipase LIPOZYME RMIM is 5% of a grease weight, 55 ℃ of reaction temperatures, 4 hours ester exchange reaction time.Again at 150-180 ℃ of following molecular clock, refining obtained human milk fat substituted thing after reaction finished.
Embodiment three:
1, extract Mekong Delta catfish fat:
Concrete extraction step is seen embodiment one.
2, the grease branch is carried:
Concrete branch is put forward step and is seen embodiment one.
3, the preparation of mixing-in fat acetoacetic ester:
Corn oil, linseed oil and coconut oil are mixed by 6: 2: 2 weight ratio, heat to 100 ℃, stir simultaneously, keep 20min, dehydration.1% heavy catalyst KOH of oil joins (mol ratio of ethanol and grease is 6: 1) in the ethanol, stirring and dissolving.Treat that grease is cooled to 60 ℃, be added with the ethanolic solution of catalyst, begin reaction, 3 hours time.Add citric acid solution (mol ratio of citric acid and KOH is 1: 1) washing.60 ℃ following vacuum suction 1-2 hour.Get end-product mixing-in fat acetoacetic ester.
4, enzyme process ester exchange:
The branch extract body component of Mekong Delta catfish and the weight ratio of the mixing-in fat acetoacetic ester that above-mentioned steps obtains are 1: 1, and the addition of lipase LIPOZYME RMIM is 5% of a grease weight, 55 ℃ of reaction temperatures, 4 hours reaction time.Again at 120-150 ℃ of following molecular clock, refining obtained human milk fat substituted thing after ester exchange reaction finished.
Embodiment four:
1, the extraction of Mekong Delta catfish fat:
Concrete extraction step is seen embodiment one.
2, the preparation of mixing-in fat acetoacetic ester:
Sunflower oil, linseed oil and palm-kernel oil are mixed by 6: 1: 2 weight ratios, heat to 100 ℃, stir simultaneously, keep 20min, dehydration.1% heavy catalyst KOH of oil joins (mol ratio of ethanol and grease is 6: 1) in the ethanol, stirring and dissolving.Treat that grease is cooled to 60 ℃, be added with the ethanolic solution of catalyst, begin reaction, 3 hours time.Add citric acid solution (mol ratio of citric acid and KOH is 1: 1) washing.60 ℃ following vacuum suction 1-2 hour.Get end-product mixing-in fat acetoacetic ester.
3, enzyme process ester exchange:
Mekong Delta catfish fat is mixed with palm oil after the ester exchange at random by 5: 5 part by weight, the weight ratio of the mixing-in fat acetoacetic ester that obtains in compound lard and the above-mentioned steps 2 is 1: 1, with the mixture of compound lard and miscella fat acetoacetic ester by being filled with the reaction column of lipase LIPOZYME RMIM, 55 ℃ of reaction temperatures, flow velocity be per hour by with the reactant of enzyme equivalent weight.Product is again at 120-150 ℃ of following molecular clock, and refining obtains human milk fat substituted thing.
Claims (10)
1. a grease that derives from the Mekong Delta catfish is characterized in that: be extraction or the catfish fat that extracts from all the other later positions of removal fillet or the branch extract body component of described catfish fat from the full fish of Mekong Delta catfish (Pangasianodon gigas).
2. the grease that derives from the Mekong Delta catfish according to claim 1, it is characterized in that: the extracting method of described catfish fat is, Mekong Delta catfish raw material is cleaned, full fish is pulverized maybe will remove behind the fillet raw material of remainder and pulverize, pass through continuously cooking then, press filtration, filtrate is used Steam Heating 20-60 minute, and centrifugal then, the crude fat that obtains dewaters, refining then promptly gets catfish fat.
3. the grease that derives from the Mekong Delta catfish according to claim 1 and 2, it is characterized in that: the branch extracting method of the branch extract body component of described catfish fat is, Mekong Delta catfish fat is heated to 55-60 ℃, after stablizing 20 minutes, be cooled to 30 ℃ with 10 ℃/hour speed, be cooled to 10-30 ℃ with 1.5-2.5 ℃/hour speed again, thermostatical crystallization 3-5 hour, aforementioned all processes add band and stir, after solid crystal to be separated out partly reaches the 10-50% of gross weight, under temperature 10-30 ℃, begin to carry out isolated by filtration, after filtering, obtain branch extract body component.
4. the grease that derives from the Mekong Delta catfish according to claim 1 is characterized in that: described Mekong Delta catfish (Pangasianodon gigas) is Ba Sha catfish (basa catfish) and short, bristly hair or beard catfish (tra catfish).
5. the described application of grease in the human milk fat substituted thing of preparation that derives from the Mekong Delta catfish of the arbitrary claim of claim 1 to 4.
6. one kind is adopted the described method that derives from the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish of the arbitrary claim of claim 1 to 4, it is characterized in that comprising the steps:
(1) extracts the fat that derives from Mekong Delta catfish (Pangasianodon gigas);
(2) the catfish fat that previous step is obtained suddenly carries out the grease branch and carries and obtain its minute extract body component under 10-30 ℃ of temperature conditions;
(3) grease that step (1) or step (2) are obtained lipase-catalyzed down and fatty acid mixed or fatty acid mixed low-carbon-ester carry out the enzyme process ester exchange reaction, or with the grease that step (1) or step (2) obtain mix with other vegetable oil resulting compound lard lipase-catalyzed down and fatty acid mixed or fatty acid mixed low-carbon-ester carry out the enzyme process ester exchange reaction, the weight ratio of described grease or compound lard and fatty acid mixed or fatty acid mixed low-carbon-ester is (2: 1)-(1: 2), ester exchange reaction promptly gets human milk fat substituted thing after resulting grease of reaction and refining are isolated in decompression distillation.
7. employing according to claim 6 derives from the method for the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish, it is characterized in that: the extracting method in described (1) step is, Mekong Delta catfish raw material is cleaned, full fish is pulverized maybe will remove behind the fillet raw material of remainder and pulverize, then through advancing continuously cooking, press filtration, filtrate is used Steam Heating 20-60 minute, and centrifugal then, the crude fat that obtains dewaters, refining then promptly gets catfish fat.
8. derive from the method for the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish according to claim 6 or 7 described employings, it is characterized in that: the temperature that described (2) step grease branch is carried is preferably 15-25 ℃.
9. derive from the method for the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish according to claim 6 or 7 described employings, it is characterized in that: described aliphatic acid low-carbon-ester is preferably fatty-acid ethyl ester.
10. derive from the method for the human milk fat substituted thing of oil and fat preparation of Mekong Delta catfish according to claim 6 or 7 described employings, it is characterized in that: the fatty acid mixed in described (3) step derives from the hydrolysate of any compound lard of soybean oil, Canola Oil, sunflower oil, corn oil, coconut oil, palm-kernel oil; Described fatty acid mixed low-carbon-ester derives from the alcoholysis product of any compound lard of soybean oil, Canola Oil, sunflower oil, corn oil, coconut oil, palm-kernel oil.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010225899 CN101940241B (en) | 2010-07-12 | 2010-07-12 | Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof |
| PCT/CN2011/077001 WO2012006934A1 (en) | 2010-07-12 | 2011-07-08 | Pangasianodon gigas oil, use thereof, and method of preparing human milk fat substitute |
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|---|---|---|---|
| CN 201010225899 CN101940241B (en) | 2010-07-12 | 2010-07-12 | Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof |
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| CN101940241A true CN101940241A (en) | 2011-01-12 |
| CN101940241B CN101940241B (en) | 2013-05-08 |
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| CN 201010225899 Active CN101940241B (en) | 2010-07-12 | 2010-07-12 | Pangasianodon gigas lipid, application and method for preparing breast milk lipid substitutes thereof |
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| Country | Link |
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| CN (1) | CN101940241B (en) |
| WO (1) | WO2012006934A1 (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012006934A1 (en) * | 2010-07-12 | 2012-01-19 | 深圳精益油脂技术有限公司 | Pangasianodon gigas oil, use thereof, and method of preparing human milk fat substitute |
| CN103689101A (en) * | 2013-12-17 | 2014-04-02 | 江苏科技大学 | Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source |
| CN103766506A (en) * | 2012-10-23 | 2014-05-07 | 丰益(上海)生物技术研发中心有限公司 | Grease composition |
| CN104862350A (en) * | 2015-05-06 | 2015-08-26 | 江南大学 | Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride |
| CN104855542A (en) * | 2015-05-06 | 2015-08-26 | 江南大学 | Preparation method of human milk alternative fat combining enzymatic acidolysis and physical mixing |
| CN105400594A (en) * | 2015-10-20 | 2016-03-16 | 广州市至润油脂食品工业有限公司 | Fresh water fish oil fractionation method |
| WO2016101891A1 (en) * | 2014-12-24 | 2016-06-30 | Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd | An oil/fat composition and a preparation method for the same |
| CN105994652A (en) * | 2016-06-08 | 2016-10-12 | 华南理工大学 | Formula milk powder for infants and young children |
| CN106359663A (en) * | 2016-08-24 | 2017-02-01 | 深圳精益油脂技术有限公司 | Breast milk fat substitute prepared from oil of tilapia, application and method |
| CN108651896A (en) * | 2018-04-24 | 2018-10-16 | 大连工业大学 | A kind of production method of instant Ba Sha fillet |
| CN108740360A (en) * | 2018-08-14 | 2018-11-06 | 广州市至润油脂食品工业有限公司 | Include the feed fat composition and its preparation method and application of Ba Sha fish oil |
| CN109077186A (en) * | 2018-08-14 | 2018-12-25 | 广州市至润油脂食品工业有限公司 | A kind of preparation method of weanling pig grease and its application in baby pig feedstuff |
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| US20020058702A1 (en) * | 1999-02-12 | 2002-05-16 | Junkaun Wang | Stabilized lipid composition, process of preparation and nutritional product containing it |
| CN101181079A (en) * | 2007-12-19 | 2008-05-21 | 湘西自治州金凤凰农业生物科技有限公司 | Spot fork-tail catfish oil and preparation method thereof |
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012006934A1 (en) * | 2010-07-12 | 2012-01-19 | 深圳精益油脂技术有限公司 | Pangasianodon gigas oil, use thereof, and method of preparing human milk fat substitute |
| CN103766506A (en) * | 2012-10-23 | 2014-05-07 | 丰益(上海)生物技术研发中心有限公司 | Grease composition |
| CN103689101A (en) * | 2013-12-17 | 2014-04-02 | 江苏科技大学 | Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source |
| CN103689101B (en) * | 2013-12-17 | 2015-08-12 | 江苏科技大学 | The preparation method of the human milk fat substituted product of synthesis in silkworm chrysalis oil source |
| WO2016101891A1 (en) * | 2014-12-24 | 2016-06-30 | Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd | An oil/fat composition and a preparation method for the same |
| CN105779141A (en) * | 2014-12-24 | 2016-07-20 | 丰益(上海)生物技术研发中心有限公司 | Grease composition and preparation method thereof |
| CN104862350A (en) * | 2015-05-06 | 2015-08-26 | 江南大学 | Method for preparing 1, 3-dilinolein-2-palmitic acid triglyceride |
| CN104855542A (en) * | 2015-05-06 | 2015-08-26 | 江南大学 | Preparation method of human milk alternative fat combining enzymatic acidolysis and physical mixing |
| WO2016176987A1 (en) * | 2015-05-06 | 2016-11-10 | 江南大学 | Preparation method for structured lipid rich in 1,3-dioleate-2-palmitic acid triglyceride |
| WO2016176988A1 (en) * | 2015-05-06 | 2016-11-10 | 江南大学 | Preparation method combining enzymatic acid hydrolysis and physical mixing for breast milk fat substitute |
| CN104855542B (en) * | 2015-05-06 | 2018-10-30 | 江南大学 | The human milk of a kind of combination enzyme process acidolysis and physical mixed substitutes the preparation method of fat |
| CN105400594A (en) * | 2015-10-20 | 2016-03-16 | 广州市至润油脂食品工业有限公司 | Fresh water fish oil fractionation method |
| CN105994652A (en) * | 2016-06-08 | 2016-10-12 | 华南理工大学 | Formula milk powder for infants and young children |
| CN106359663A (en) * | 2016-08-24 | 2017-02-01 | 深圳精益油脂技术有限公司 | Breast milk fat substitute prepared from oil of tilapia, application and method |
| CN108651896A (en) * | 2018-04-24 | 2018-10-16 | 大连工业大学 | A kind of production method of instant Ba Sha fillet |
| CN108740360A (en) * | 2018-08-14 | 2018-11-06 | 广州市至润油脂食品工业有限公司 | Include the feed fat composition and its preparation method and application of Ba Sha fish oil |
| CN109077186A (en) * | 2018-08-14 | 2018-12-25 | 广州市至润油脂食品工业有限公司 | A kind of preparation method of weanling pig grease and its application in baby pig feedstuff |
| CN109077186B (en) * | 2018-08-14 | 2022-03-15 | 广州市至润油脂食品工业有限公司 | Preparation method of oil for weaned piglets and application of oil in piglet feed |
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| CN101940241B (en) | 2013-05-08 |
| WO2012006934A1 (en) | 2012-01-19 |
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