CN101899536B - Application of bioflocculant synthesized by bacillus licheniformis in refining sugar - Google Patents
Application of bioflocculant synthesized by bacillus licheniformis in refining sugar Download PDFInfo
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- CN101899536B CN101899536B CN 201010269454 CN201010269454A CN101899536B CN 101899536 B CN101899536 B CN 101899536B CN 201010269454 CN201010269454 CN 201010269454 CN 201010269454 A CN201010269454 A CN 201010269454A CN 101899536 B CN101899536 B CN 101899536B
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Abstract
The invention provides application of a safe, nontoxic and pollution-free bioflocculant synthesized by bacillus licheniformis in refining sugar and relates to a bioflocculant. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on January 14th, 2009, and the accession number of the bacillus licheniformis is CGMCC No. 2876. The bioflocculant synthesized by the bacillus licheniformis can be applied to refining sugar.
Description
Technical field
The present invention relates to a kind of biological flocculant, especially relate to the synthetic application of biological flocculant in sugaring of Bacillus licheniformis.
Background technology
Biological flocculant is a class by being difficult for the aggegations such as solid suspended particle, somatic cells and colloidal particle of degraded, the special polymer meta-bolites of precipitation in the made liquid of microorganisms, mainly contains the macromolecular compounds such as albumen, mucopolysaccharide, Mierocrystalline cellulose and DNA.Compare with traditional inorganic and organic synthesis polymeric flocculant, biological flocculant has character and the advantage of many uniquenesses: 1. nontoxic, safe; 2. easily be degraded by microorganisms non-secondary pollution; 3. have very strong turbidity removal and decoloration performance; 4. applied widely; 5. be easy to solid-liquid separation, form throw out few; 6. some biological flocculant also has pH and thermostability is strong, the characteristics that consumption is little (Takagi H, Kadowaki K.Poly-galactosamine producedby amicroorganism.Nat Technol.1985a, 49 (3): 121-128).
The applied range of biological flocculant, such as, the application in wastewater treatment comprises removal, the waste water decoloring of suspended substance in the waste water and removes biochemistry, chemical oxygen demand (COD) etc.; The application that separating substances is purified, comprise foodstuffs industry, separation of heavy metal ions and ore dressing (Zheng Huaili etc. biological flocculant and flocculation technique, Beijing: Chemical Industry Press, 2004,230-278).
In the sugar cane juice sugar industry, flocculation agent is used to remove the various nonsugars such as the colloid that affects crystallization of sucrose in the sugarcane juice, suspended particle, pigment, to obtain lower, clear, the pure purified juice of colour.At present, most of factory all uses organic synthesis polymeric flocculant polyacrylamide to be used for clarification and the decolouring of sugarcane juice.2004, (Xie Caifeng, the Huang Yuegang such as Xie Caifeng, Yang Liansheng. amphiprotic polyacrylamide is processed inferior method sugar refinery mixing juice, Chinese beet sugar industry, 2004 (3), 1:1-4) process the Cane Mixed Juice in inferior method sugar refinery with polyacrylamide, investigate its clarifying effect.But because the polyacrylamide difficult degradation easily causes secondary pollution and polymerization single polymerization monomer acrylamide to have strong neurotoxicity, present many countries and field have been forbidden or have been limited the quantity of and use this type of flocculation agent.The polyacrylamide Separum Nplo maximum permitted concentration of using such as U.S.'s approval is 1mg/mL; Britain regulation PAM adds dosage and on average must not surpass 0.5mg/mL, and maximum adds dosage must not surpass 1mg/mL.
Along with the enhancing of Economic development and people's environmental consciousness, seek a kind of cheapness, efficient, nontoxic, again can complete biodegradable, the novel green flocculation agent that do not produce secondary pollution become the demand in epoch.At present domestic more existing be applied to the report of sugar industry about biological flocculant.2004, (the Wu Chunlan such as Wu Chunlan, Lu Jiajiong etc. the preliminary study that microbial flocculant is used in Sugar Industry, Sugar Industry, 2004,2:22-29) preliminary study derives from biological flocculant that the microbial fermentation of DSMZ of the Chinese Academy of Sciences produces to the clarifying effect of sulfurous acid factory Cane Mixed Juice, the result shows, but the settling velocity of microbial flocculant Effective Raise mixing juice, and compression filter mud volume also has decolorizing effect, wherein, the consumption of biological flocculant is 2mL.Subsequently, 2006, (the Li Nan such as Li Nan, Deng Zhinian etc. the applied research of microbial flocculant in cane sugar factory, industrial microorganism, 2006,36 (1): 43-46) from active sludge, isolate the microorganism strains that a strain is initially identified as Verticillium, the biological flocculant that utilizes this bacterial strain to produce is processed sulfurous acid factory Cane Mixed Juice, investigates this biological flocculant to the clarify and decolorize effect of sugar cane juice, and this result of study shows, the flocculating rate of microbial fermentation solution in the sugarcane juice settling test is 69.2%, color value of clear juice IU is 87.4, and wherein, the consumption of biological flocculant is 3mL.But abroad, have no the report that relevant biological flocculant is applied to sugar industry.
Because refractory organics and the neurotoxicity of organic synthesis polymeric flocculant acrylamide, the insecurity that it is used in sugar industry and people pay attention to the hope of food safety and disagree, therefore, develop that a kind of safety non-toxic, flocculation activity are high, non-secondary pollution, the novel flocculant that with low cost, output is high is applied to cane sugar manufacture industry, has been trend of the times.
The applicant provides a kind of and has higher flocculation activity in the patent application of publication number for CN101503709, raw materials cost is low, the industrial application potentiality the are larger Bacillus licheniformis that utilizes prepares the method for biological flocculant.Microorganism is Bacillus licheniformis (Bacillus licheniformis), this microorganism was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 01 14th, 2009, registered on the books and be numbered CGMCC No.2876 in the preservation center.After the method is forwarded to the seed culture medium cultivation with the lawn on the fresh inclined-plane, being forwarded to fermention medium cultivates, get the biological flocculant fermented liquid, the centrifugal precipitation of removing is collected supernatant liquor, adds ethanol and leaves standstill rear centrifugal, remove supernatant liquor, in throw out, add again dehydrated alcohol, stir, centrifugal, remove supernatant liquor, in the ethanol throw out, add cetyl trimethylammonium bromide, centrifugal, remove supernatant liquor and get throw out, throw out is dissolved in the NaCl solution, add dehydrated alcohol, stir, leave standstill, the centrifugal supernatant liquor of removing gets throw out, and the throw out vacuum freezedrying is obtained the sterling biological flocculant.
Summary of the invention
The object of the invention is to the problems such as refractory organics, neurotoxicity for the employed polyacrylamide of present cane sugar manufacture industry, provide a kind of safe, nontoxic, free of contamination Bacillus licheniformis the synthetic application of biological flocculant in sugaring.
The microorganism that the present invention adopts is Bacillus licheniformis (Bacillus licheniformis), this microorganism has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 01 14th, 2009, register on the books and be numbered CGMCC No.2876 (publication number is CN101503709) in the preservation center.
The applicant discloses a kind of method of utilizing Bacillus licheniformis to prepare biological flocculant in publication number is the application for a patent for invention of CN101503709, after the method is forwarded to the seed culture medium cultivation with the lawn on the fresh inclined-plane, being forwarded to fermention medium cultivates, get the biological flocculant fermented liquid, the centrifugal precipitation of removing is collected supernatant liquor, adding ethanol leaves standstill rear centrifugal, removes supernatant liquor, adds dehydrated alcohol in throw out again, stir, centrifugal, remove supernatant liquor, in the ethanol throw out, add cetyl trimethylammonium bromide, centrifugal, remove supernatant liquor and get throw out, throw out is dissolved in the NaCl solution, add dehydrated alcohol, stir, leave standstill, the centrifugal supernatant liquor of removing gets throw out, and the throw out vacuum freezedrying is obtained the sterling biological flocculant.
The synthetic biological flocculant of described Bacillus licheniformis can be used in sugaring.
Concrete operation step of the present invention is as follows:
1) fermentation of biological flocculant is synthetic
After bacterium colony on the fresh flat board is forwarded to seed culture medium and cultivates, be forwarded to fermention medium and cultivate, get the biological flocculant fermented liquid;
2) cane juice clarification decoloration process
Get adding phosphoric acid 3mL in the 100mL Cane Mixed Juice, transferring pH with milk of lime is 6.8, heats while stirring, and keeps 65 ℃ of 2min, adds H
28O
30.4mL, again with lime slurry neutralization to pH6.8～7.2, again be heated to boiling, keep 2min, add flocculation agent 7～21U/mL, stir, leave standstill, in 1～20min, measure sedimentation filter mud height every 1min; Supernatant liquid filters, and leaches clearly juice accent pH7.0, measures hammer degree, total reducing sugar, reducing sugar, turbidity, colour.
Wherein, hammer degree, turbidity, colour press cane sugar manufacture chemistry management analysis method measure (Zheng Changgeng, Lee Yong etc. cane sugar manufacture chemistry management analysis method, 1995, Sugar Industry quality surveillance inspection center of the Commission of Light Industry compiles); Reducing sugar is pressed the DNS method and is measured; Total reducing sugar is pressed sulfuric acid-phynol method and is measured.
Description of drawings
Fig. 1 is that settling velocity contrasted after embodiment of the invention polyacrylamide PAM and biological flocculant XMMBF processed sugar cane juice.In Fig. 1, X-coordinate is settling time/min, and ordinate zou is filter mud volume/mL; ■ PAM, ● be biological flocculant.
Fig. 2 is purity, percent of decolourization, turbidity removal rate, the contrast of sucrose yield of sugarcane juice after embodiment of the invention polyacrylamide PAM and biological flocculant are processed.In Fig. 2, X-coordinate is classification, is followed successively by purity, percent of decolourization, turbidity removal rate, sucrose yield, and ordinate zou is ratio/%; A is PAM, and b is biological flocculant.
Fig. 3 is in the embodiment of the invention and purity, percent of decolourization, turbidity removal rate, the sucrose yield of the rear sugarcane juice of pH value 7.2 processing.In Fig. 3, X-coordinate is classification, is followed successively by purity, percent of decolourization, turbidity removal rate, sucrose yield, and ordinate zou is ratio/%.
Fig. 4 is purity, percent of decolourization, turbidity removal rate, the sucrose yield of sugarcane juice after embodiment of the invention biological flocculant 16U/mL processes.In Fig. 4, X-coordinate is classification, is followed successively by purity, percent of decolourization, turbidity removal rate, sucrose yield, and ordinate zou is ratio/%.
Embodiment
The present invention is further illustrated in connection with accompanying drawing for following examples.
Embodiment 1: process sugar cane juice with polyacrylamide and biological flocculant XMMBF respectively, investigate the clarify and decolorize effect to sugar cane juice.Flocculation system is: get adding phosphoric acid 3mL in the 100mL Cane Mixed Juice, transferring pH with milk of lime is 6.8, heats while stirring, and keeps 65 ℃ of 2min, adds H
2SO
30.4mL, again with lime slurry neutralization to pH7.0, again be heated to boiling, keep 2min, add flocculation agent (polyacrylamide PAM concentration is that 1.5ppm or biological flocculant XMMBF are 18U/mL), stir, leave standstill, in 20min, measure sedimentation filter mud height every 1min; Supernatant liquid filters, and leaches clearly juice accent pH7.0, measures hammer degree, total reducing sugar, reducing sugar, turbidity, colour, and the result as illustrated in fig. 1 and 2.From Fig. 1 and 2 can find out adopt biological flocculant to process after, settling velocity is very fast, and purity is higher, good decolorizing effect, the sucrose loss amount is less.
Embodiment 2: flocculating conditions as described in Example 1, investigate in the difference and the pH value on the impact of cane juice clarification decolorizing effect.Its difference is to add after the sulfurous acid, with lime slurry neutralization to pH7.2.The result as shown in Figure 3.
Embodiment 3: flocculating conditions as described in Example 1, investigate in the difference and the pH value on the impact of cane juice clarification decolorizing effect.Its difference is to add after the sulfurous acid, with lime slurry neutralization to pH6.8.
Embodiment 4: flocculating conditions as described in Example 1, investigate different biological flocculant XMMBF add-ons to the impact of cane juice clarification decolorizing effect.The amount that its difference is to add biological flocculant XMMBF is 16U/mL.The result as shown in Figure 4.
Embodiment 5: flocculating conditions as described in Example 1, investigate different biological flocculant XMMBF add-ons to the impact of cane juice clarification decolorizing effect.The amount that its difference is to add biological flocculant XMMBF is 7U/mL.
Embodiment 6: flocculating conditions as described in Example 1, investigate different biological flocculant XMMBF add-ons to the impact of cane juice clarification decolorizing effect.The amount that its difference is to add biological flocculant XMMBF is 8U/mL.
Embodiment 7: flocculating conditions as described in Example 1, investigate different biological flocculant XMMBF add-ons to the impact of cane juice clarification decolorizing effect.The amount that its difference is to add biological flocculant XMMBF is 12U/mL.
Embodiment 8: flocculating conditions as described in Example 1, investigate different biological flocculant XMMBF add-ons to the impact of cane juice clarification decolorizing effect.The amount that its difference is to add biological flocculant XMMBF is 21U/mL.
Embodiment 9: flocculating conditions as described in Example 1, investigate the different point of additions of biological flocculant to the impact of cane juice clarification decolorizing effect.Its difference is to change the point of addition of biological flocculant, i.e. 65 ℃ of heating also keep adding biological flocculant XMMBF after the 2min.
Embodiment 10: flocculating conditions as described in Example 1, investigate the different point of additions of biological flocculant to the impact of cane juice clarification decolorizing effect.Its difference is to change the point of addition of biological flocculant, namely before being heated to boiling, adds biological flocculant XMMBF in having transferred and behind the pH.
Embodiment 11: flocculating conditions as described in Example 1, investigate the change Heating temperature second time to the impact of cane juice clarification decolorizing effect.Its difference is that the temperature of heating is 70 ℃ for the second time.
Embodiment 12: flocculating conditions as described in Example 1, investigate the change Heating temperature second time to the impact of cane juice clarification decolorizing effect.Its difference is that the temperature of heating is 80 ℃ for the second time.
Embodiment 13: flocculating conditions as described in Example 1, investigate the change Heating temperature second time to the impact of cane juice clarification decolorizing effect.Its difference is that Heating temperature is 90 ℃ for the second time.
Claims (1)
1. the synthetic application of biological flocculant in sugaring of Bacillus licheniformis, described Bacillus licheniformis (Bacillus licheniformis) was preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 01 14th, 2009, registered on the books and be numbered CGMCC No.2876 in the preservation center; Utilize Bacillus licheniformis to prepare the method for biological flocculant as follows: after the lawn on the fresh inclined-plane is forwarded to seed culture medium and cultivates, being forwarded to fermention medium cultivates, get the biological flocculant fermented liquid, the centrifugal precipitation of removing is collected supernatant liquor, adds ethanol and leaves standstill rear centrifugal, remove supernatant liquor, in throw out, add again dehydrated alcohol, stir, centrifugal, remove supernatant liquor, in the ethanol throw out, add cetyl trimethylammonium bromide, centrifugal, remove supernatant liquor and get throw out, throw out is dissolved in the NaCl solution, add dehydrated alcohol, stir, leave standstill, the centrifugal supernatant liquor of removing gets throw out, and the throw out vacuum freezedrying is obtained the sterling biological flocculant.
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102260729B (en) * | 2011-06-24 | 2013-03-13 | 哈尔滨工业大学 | Bioflocculant fermentation method with mycelium pellet as vector |
| CN103255094B (en) * | 2013-05-20 | 2015-04-22 | 厦门六维生物科技有限公司 | Method for preparing bacillus licheniformis |
| CN104480036B (en) * | 2014-11-21 | 2017-06-06 | 华南理工大学 | Microbial flocculant proteus vulgaris and its application in cane juice clarification |
| CN105063244A (en) * | 2015-08-07 | 2015-11-18 | 广西南宁派腾科技有限公司 | Technology for decolorization and impurity removal of saccharum officinarum juice |
| CN105624176B (en) * | 2016-01-15 | 2019-02-05 | 厦门大学 | It is overexpressed engineering bacteria and the building of UDPglucose pyrophosphorylase gene |
| CN105624080B (en) * | 2016-01-15 | 2019-02-05 | 厦门大学 | Produce the bacillus licheniformis genetic engineering bacterium and its construction method of polysaccharide flocculant |
| CN106636151A (en) * | 2017-02-23 | 2017-05-10 | 厦门大学 | Engineering bacterium for over-expressing glycosyltransferase gene and construction method |
| CN110241267A (en) * | 2019-06-25 | 2019-09-17 | 广西浦北县湘桂糖业有限责任公司 | A kind of bioremediation of no sulphur cane juice clarification |
| US11826676B2 (en) * | 2021-08-19 | 2023-11-28 | Komline-Sanderson Corporation | Flocculant composition and method of forming same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU825635A1 (en) * | 1979-06-29 | 1981-04-30 | Mo T I Pishchevoj Promy | Method of clarifying sugar-containing solution |
| US6156563A (en) * | 1998-01-29 | 2000-12-05 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Method for clarifying cane sugar juice |
| CN1693483A (en) * | 2005-06-14 | 2005-11-09 | 福建师范大学 | Method of clarifying saccharic crop pressed juice mixture by using bentonite-chitin composite flocculant |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5257342A (en) * | 1975-10-21 | 1977-05-11 | Nansei Tougiyou Kk | Process for cleaning raw sugar juice |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU825635A1 (en) * | 1979-06-29 | 1981-04-30 | Mo T I Pishchevoj Promy | Method of clarifying sugar-containing solution |
| US6156563A (en) * | 1998-01-29 | 2000-12-05 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Method for clarifying cane sugar juice |
| CN1693483A (en) * | 2005-06-14 | 2005-11-09 | 福建师范大学 | Method of clarifying saccharic crop pressed juice mixture by using bentonite-chitin composite flocculant |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
Non-Patent Citations (1)
| Title |
|---|
| 陈咏竹等.γ-多聚谷氨酸的性质、发酵生产.《微生物学通报》.2004,第31卷(第1期),125. * |
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