CN101891539A - Water hyacinth biogas slurry-based plant source foliar insecticide-fertilizer, preparation method and application - Google Patents
Water hyacinth biogas slurry-based plant source foliar insecticide-fertilizer, preparation method and application Download PDFInfo
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Abstract
The invention discloses a water hyacinth biogas slurry-based plant source foliar insecticide-fertilizer. The plant source foliar insecticide-fertilizer comprises the following components in percentage by weight: 0.1 to 1 percent of eugenol, 0.5 to 3 percent of water hyacinth biogas slurry, 0.1 to 1 percent of absolute ethanol and the balance of water. The invention also discloses a preparation method and the application of the plant source foliar insecticide-fertilizer. The water hyacinth biogas slurry-based plant source foliar insecticide-fertilizer of the invention has an active ingredient which is derived from a plant or an extract thereof with an insecticidal function and a nutritional ingredient which is derived from industrial, agricultural and animal husbandry production waste treated by innocuous technology, has effects of prompting plant growth and development, increasing production and yield, improving quality, improving nutrient utilization rate, preventing and controlling injurious insects, non-pollution and the like, and is suitable for non-polluted, green and organic agriculture product production. The foliar insecticide-fertilizer has the characteristics of remarkable application effect, easily-obtained raw materials, simple production process, great market potential, obvious economic benefit and social benefit, and very board application prospect.
Description
Technical field
The invention belongs to field of agricultural production technologies, be specifically related to a kind of plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry and preparation method thereof and production method.
Background technology
For a long time, China still is in a kind of " embarrassment " position as " agricultural chemicals big country ", promptly produce and the riskiest pesticide that uses far above world average level, eco-friendly biological pesticide is corresponding to world average level.Because being used to prevent and treat the agricultural chemicals of corps diseases, China mostly is chemical pesticide extremely, yet the life-time service chemical pesticide causes a series of adverse consequencess, mainly show several respects: the one, ecotope constantly worsens, chemical pesticide not only has restraining effect to disease, has also destroyed the natural eubiosis simultaneously; The 2nd, the life-time service chemical pesticide makes disease produce resistance, thereby has increased the amount of application and the access times of agricultural chemicals; The 3rd, because a large amount of uses of chemical pesticide cause agricultural chemicals accumulation gradually in environment, produced the pesticidal contamination environmental problem, especially soil quality constantly reduces, and has reduced the sustainable use ability of soil; The 4th, the poisoning of the residual initiation of chemical pesticide happens occasionally, because of most of chemical pesticide residual period, is longer, agricultural-food particularly vegetables, melon and fruit be vulnerable to severe contamination, people's the healthy potential threat that just exists.Simultaneously because for a long time to the too dependence of chemical fertilizer, excessive use, thereby produced the series of negative influence: the one, using of a large amount of chemical fertilizer descends soil quality, the soil salinization, harden, phenomenon such as acidifying occurs; The 2nd, uneven fertilising causes soil nutrient unbalance, and crop physiological disease takes place, and from malicious product accumulation aggravation, soil microflora is unbalance; The 3rd, quality of agricultural product constantly descends; The 4th, superfluous nutrient enters water body and has increased the weight of body eutrophication.
The Phytophthora capsici disease is a kind of worldwide soil-borne disease, has a strong impact on the agriculture production of China and countries in the world, causes enormous economic loss.By the medicine fertilizer of making after the improvement of vegetable source natural compound, to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, no poisoning, advantages such as the non-resistant property of medicine on the one hand; Owing to nutritive element balance in the medicine fertilizer, can effectively reduce the usage quantity of chemical fertilizer on the other hand; The 3rd, in the control disease, can effectively improve quality of agricultural product.Therefore, manage to take effective technical measures, develop out the focus that environmentally friendly medicine fertilizer just becomes research of agricultural science.
Summary of the invention
Technical problem to be solved by this invention provides a kind of plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry, and this medicine fertilizer is a kind of friendly medicine fertilizer of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry.
The technical problem that the present invention will solve at last provides the application of above-mentioned medicine fertilizer.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry, it comprises the component of following weight percentage: Eugenol 0.1～1%, water hyacinth biogas slurry 0.5～3%, dehydrated alcohol 0.1～1%, all the other are water.
Wherein, described water hyacinth biogas slurry prepares as follows and gets: after Herba Eichhorniae is pulverized, 0.5～2 times the water that adds Herba Eichhorniae weight, adopt anaerobic activated sludge or existing water hyacinth biogas slurry to carry out microbial inoculant, inoculum size is 10～40% (v/v), 25～50 ℃ of bottom fermentations 10～15 days, extract natural pond liquid, remove solid impurity after filtration promptly.
The preparation method of above-mentioned plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry adds water hyacinth biogas slurry in the entry earlier and mixes, and adds in the above-mentioned mixing solutions after Eugenol being dissolved in dehydrated alcohol then again.
The above-mentioned application of plant source foliar insecticide-fertilizer in promoting plant growth based on water hyacinth biogas slurry.
Wherein, described crop is capsicum, summer squash, cucumber, eggplant, tomato or green vegetables.
The above-mentioned application of plant source foliar insecticide-fertilizer in control crop Phytophthora capsici disease based on water hyacinth biogas slurry.
Wherein, described crop is capsicum, summer squash, cucumber, eggplant, tomato, watermelon, pumpkin or potato.
Beneficial effect: its effective component of plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry of the present invention derives from plant or its extract with insecticidal function, waste is produced in industrial or agricultural, livestock industry that nutritive ingredient derives from after innoxious technical finesse, have promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.This Pesticidal foliage fertilizer effect significantly, starting material are easy to get, and production technique is simple, market potential is big, economic benefit and social benefit are obvious, has very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, processing condition and result thereof only are used to illustrate the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
The preparation of water hyacinth biogas slurry:
After Herba Eichhorniae pulverized, add 1.2 times water of Herba Eichhorniae weight, adopt anaerobic activated sludge to carry out microbial inoculant, inoculum size is 15% (v/v), 30 ℃ of bottom fermentations 10 days, extracts natural pond liquid, removes solid impurity after filtration promptly.Wherein contain following component by mass percent: NH in the liquid of natural pond
4 +0.038%, NO
3 -0.037%, P
2O
50.022%, K
2O0.152%, amino acid 0.252%, organic matter 0.209%, water content 99.29%.
The component that comprises following weight percentage based on the plant source foliar insecticide-fertilizer of water hyacinth biogas slurry: Eugenol 0.3%, water hyacinth biogas slurry 1.5%, dehydrated alcohol 0.44%, all the other are water.
Preparation method based on the plant source foliar insecticide-fertilizer of water hyacinth biogas slurry: the water hyacinth biogas slurry of formula ratio is added in the entry mix earlier, Eugenol is dissolved in adds again behind the dehydrated alcohol in the above-mentioned mixing solutions (promptly dilute water hyacinth biogas slurry) then.
Embodiment 2:
With the method for embodiment 1, the prescription difference of different the are based on plant source foliar insecticide-fertilizer of water hyacinth biogas slurry, it comprises the component of following weight percentage: Eugenol 1%, water hyacinth biogas slurry 3%, dehydrated alcohol 1%, all the other are water.
Embodiment 3:
With the method for embodiment 1, the prescription difference of different the are based on plant source foliar insecticide-fertilizer of water hyacinth biogas slurry, it comprises the component of following weight percentage: Eugenol 0.1%, water hyacinth biogas slurry 0.5%, dehydrated alcohol 0.1%, all the other are water.
Embodiment 4: based on the plant source foliar insecticide-fertilizer of water hyacinth biogas slurry to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1.5% (w/w) water hyacinth biogas slurry (water hyacinth biogas slurry that makes by embodiment 1 method), 98.5% (w/w) water.
Formula I I:0.3% (w/w) Eugenol, dehydrated alcohol 0.4%, 99.3% (w/w) water.
The biological Pesticidal foliage fertilizer prescription of the plant-sourced of formula I II: embodiment 1.
Seed is with the H of 0.3% (w/w)
2O
2After the sterilization, clean, select the culture dish (diameter 15cm) that homogeneous, the normal seed of form place shop 3 metafiltration paper then, train seedling with distilled water.5d after waiting to emerge, the material of choosing the growing way unanimity is transferred to it in plastic tub alms bowl, behind the seedling age 20d, sprays with containing different treatment liquid respectively, and each is handled and repeats 3 times.Treatment solution sprays once every 5d, samples behind the 60d, measures and respectively handles green vegetables relevant physiological biochemical indicator.(annotate: contrast CK is a clear water)
Measuring method
Fresh weight is measured: after the off-test, every basin is the consistent seedling of 3 upgrowth situations of picking at random, measures its fresh weight with the clean back of blotting of deionized water then.
Protein content determination: close generation method with reference to Lee, adopt the Xylene Brilliant Cyanine G method.(Li Hesheng. plant physiology and biochemistry experimental principle and technology. Beijing Higher Education Publishing House, 2000)
Nitrate content is measured: with reference to methods such as Lu Qiming, measure and adopt ultraviolet spectrophotometry.(Lu Qiming, Chen Min, Liao Zongwen. the improvement of determined by ultraviolet spectrophotometry nitrate nitrogen on vegetables. Agricultural University Of South China's journal, 1997,8 (4): 104-106.)
Vc measures: with reference to high friend's method, get about testing sample 2g, add a small amount of 2% hydrochloric acid, make homogenate in tissue mashing machine, move in the 100mL volumetric flask, to scale, absorbent cotton filters with 1% oxalic acid constant volume, get 10mL filtrate, with the 2,6 dichlorophenol indophenol solution titration determination Vc content of having demarcated.(high friend. the agrochemistry common analysis. Xi'an: Shaanxi science tech publishing house, 1980.)
Soluble sugar is measured: with reference to the Zou Qi method, adopt the anthrone colorimetry.(Zou Qi. plant physiology experiment instructs. Beijing: Chinese agriculture press, 2000.)
Table 1 different treatment is to the influence of green vegetables, capsicum, cucumber, tomato biomass and quality
As shown in Table 1, green vegetables are handled back fresh weight, protein content, Vc content and soluble sugar in formula I and are compared respectively according to having increased by 15.6%, 45.1%, 39.9% and 14.1%, and the nitrate content comparison is according to reducing 11.8%; Formula I I handles back green vegetables fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 6.3%, 32.9%, 19.8% and 5.1%, and the nitrate content comparison is according to reducing 2.2%; Formula I II handles back fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 31.2%, 59.8%, 52.3% and 20.5%, and the nitrate content comparison is according to reducing 17.3%.
Capsicum is handled back fresh weight, protein content, Vc content and soluble sugar in formula I and compares respectively according to having increased by 43.2%, 12.0%, 44.9% and 46.6%, and the nitrate content comparison is according to reducing 22.5%; Formula I I handles back green vegetables fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 18.3%, 7.5%, 9.7% and 17.9%, and the nitrate content comparison is according to reducing 7.8%; Formula I II handles back fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 65.9%, 20.7%, 60.8% and 72.0%, and the nitrate content comparison is according to reducing 27%.
Cucumber is handled back fresh weight, protein content, Vc content and soluble sugar in formula I and compares respectively according to having increased by 24.2%, 25.5%, 39.4% and 28.2%, and the nitrate content comparison is according to reducing 23.7%; Formula I I handles back green vegetables fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 11.0%, 19.3%, 32.7% and 15.2%, and the nitrate content comparison is according to reducing 13.0%; Formula I II handles back fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 37.6%, 39.2%, 47.3% and 36.4%, and the nitrate content comparison is according to reducing 53.9%.
Tomato is handled back fresh weight, protein content, Vc content and soluble sugar in formula I and compares respectively according to having increased by 9.0%, 13.9%, 44.4% and 22.6%, and the nitrate content comparison is according to reducing 10.5%; Formula I I handles back green vegetables fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 1.9%, 2.9%, 18.1% and 8.3%, and the nitrate content comparison is according to reducing 2.4%; Formula I II handles back fresh weight, protein content, Vc content and soluble sugar and compares respectively according to having increased by 14.3%, 19.3%, 64.2% and 35.2%, and the nitrate content comparison is according to reducing 16.3%.
On having as can be known, with formula I II handle to green vegetables, capsicum, cucumber and tomato biomass and quality to improve effect best.
Embodiment 5: based on the plant source foliar insecticide-fertilizer of water hyacinth biogas slurry at preventive and therapeutic effect to crop Phytophthora capsici disease.
Formula I: 1.5% (w/w) water hyacinth biogas slurry (water hyacinth biogas slurry that makes by embodiment 1 method), 98.5% (w/w) water.
Formula I I:0.3% (w/w) Eugenol, dehydrated alcohol 0.4%, 99.3% (w/w) water.
The biological Pesticidal foliage fertilizer prescription of the plant-sourced of formula I II: embodiment 1.
Seed is with the H of 0.3% (w/w)
2O
2After the sterilization, clean, select the culture dish (diameter 15cm) that homogeneous, the normal seed of form place shop 3 metafiltration paper then, train seedling with distilled water.5d after waiting to emerge, the material of choosing the growing way unanimity is transferred to it in plastic tub alms bowl, and behind the seedling age 20d, inoculation sprays with containing different treatment liquid respectively, and each is handled and repeats 3 times.Treatment solution sprays once every 5d, samples after company sprays 2 times.(annotate: contrast CK is a clear water)
Measuring method
The mensuration of disease index and prevention effect: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the prevention effect of graw mold of tomato. the Henan agricultural sciences, and 2010,4:77-79.)
As shown in Table 2, on capsicum, formula I is 28.09% to the preventive effect of Phytophthora capsici; Formula I I is 47.59% to the preventive effect of Phytophthora capsici; Formula I II is 72.16% to the preventive effect of Phytophthora capsici.
For cucumber, formula I is 25.82% to the preventive effect of Phytophthora capsici; Formula I I is 40.83% to the preventive effect of Phytophthora capsici; Formula I II is 65.30% to the preventive effect of Phytophthora capsici.
For tomato, formula I is 24.02% to the preventive effect of Phytophthora capsici; Formula I I is 43.83% to the preventive effect of Phytophthora capsici; Formula I II is 67.55% to the preventive effect of Phytophthora capsici.
As from the foregoing, handle best with formula I II to the prevention effect of Phytophthora capsici on capsicum, cucumber and the tomato.
Table 2 different treatment is to Phytophthora capsici prevention effect on capsicum, cucumber, the tomato
Claims (7)
1. plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry is characterized in that it comprises the component of following weight percentage: Eugenol 0.1～1%, and water hyacinth biogas slurry 0.5～3%, dehydrated alcohol 0.1～1%, all the other are water.
2. the plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry according to claim 1, it is characterized in that described water hyacinth biogas slurry prepares as follows and gets: after Herba Eichhorniae is pulverized, 0.5～2 times the water that adds Herba Eichhorniae weight, adopt anaerobic activated sludge or existing water hyacinth biogas slurry to carry out microbial inoculant, inoculum size is 10～40% (v/v), 25～50 ℃ of bottom fermentations 10～15 days, extract natural pond liquid, remove solid impurity after filtration promptly.
3. the preparation method of the described plant source foliar insecticide-fertilizer based on water hyacinth biogas slurry of claim 1 is characterized in that earlier water hyacinth biogas slurry being added in the entry mixing, and adds in the above-mentioned mixing solutions after Eugenol being dissolved in dehydrated alcohol then again.
4. the described application of plant source foliar insecticide-fertilizer in promoting plant growth of claim 1 based on water hyacinth biogas slurry.
5. application according to claim 4 is characterized in that described crop is capsicum, summer squash, cucumber, eggplant, tomato or green vegetables.
6. the described application of plant source foliar insecticide-fertilizer in control crop Phytophthora capsici disease of claim 1 based on water hyacinth biogas slurry.
7. application according to claim 6 is characterized in that described crop is capsicum, summer squash, cucumber, eggplant, tomato, watermelon, pumpkin or potato.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108450259A (en) * | 2018-06-07 | 2018-08-28 | 和县海豪蔬菜种植有限责任公司 | A kind of implantation methods of high yield high nutrition green vegetables |
| CN113912461A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Foliar fertilizer for resisting plant leaf fungal diseases |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108513897B (en) * | 2018-03-25 | 2020-12-15 | 浙江鼎兴企业管理有限公司 | Underwater cultivation method of scindapsus aureus |
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Patent Citations (5)
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| EP1623623A1 (en) * | 2003-05-13 | 2006-02-08 | Idemitsu Kosan Co., Ltd. | Antibacterial antiviral composition |
| CN101036459A (en) * | 2007-04-26 | 2007-09-19 | 江苏省农业科学院 | Eugenol micro-emulsion |
| CN101275150A (en) * | 2008-05-13 | 2008-10-01 | 江苏省农业科学院 | Fermentation method for changing methane into resource by using water hyacinth and application of product produced thereby |
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| CN113912461A (en) * | 2021-11-18 | 2022-01-11 | 海南大学 | Foliar fertilizer for resisting plant leaf fungal diseases |
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