CN101884666A - Chinese medicinal composition for treating cardiovascular and cerebrovascular diseases, and preparation method and application thereof - Google Patents
Chinese medicinal composition for treating cardiovascular and cerebrovascular diseases, and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a Chinese medicinal composition for preventing and treating cardiovascular and cerebrovascular diseases, and a preparation method and application thereof. In the Chinese medicinal composition, erigeron, panax ginseng or panax ginseng stem leaf and root of red-rooted salvia are used as raw material medicaments, active ingredients in the erigeron, the panax ginseng or the panax ginseng stem leaf and the root of red-rooted salvia are extracted respectively, and required formulations are prepared by the conventional preparation method of each formulation. The Chinese medicinal composition is used for preventing and treating diseases such as cerebral infarction, cerebral ischemia, coronary diseases, angina, myocardial ischemia, myocardial infarction, heart failure or arrhythmia and the like. In the Chinese medicinal composition, the root of red-rooted salvia, the panax ginseng and the erigeron are combined, so the curative effect is superior to the additive effect of independent medicaments, and the Chinese medicinal composition has the advantages of improving clinical curative effect along with no toxic or side effect and high safety. Compared with the prior art, the preparation method has the advantages of easy implementation, production cost reduction, high medicinal utilization ratio and excellent curative effect, and is the top choice in clinic.
Description
Technical field
The invention belongs to the field of Chinese medicines, Chinese medicine composition of specifically a kind of prevention and treatment cardiovascular and cerebrovascular disease and its production and use.
Background technology
Cardiovascular and cerebrovascular disease is a kind of serious threat mankind, the commonly encountered diseases of middle-aged and elderly people health more than 50 years old particularly, have " four high by more than one (being sickness rate height, disability rate height, mortality rate height, relapse rate height; complication is many) " though characteristics use most advanced, perfect at present treatment means, still can have the cerebrovascular accident survivor 50% or more to live and can not take care of oneself fully! Every year is died from the number of cardiovascular and cerebrovascular disease up to 1,500 ten thousand people in the whole world, occupies various causes of the death first place.
Radix Ginseng has strongly invigorating primordial QI, and multiple arteries and veins takes off admittedly, and invigorating the spleen to benefit the lung promotes the production of body fluid, the effect of calming the nerves, and the synergism that wherein contained different activities becomes branch to produce has extraordinary regulating and controlling effect to human immune system and cardio-cerebrovascular, has very strong benefiting action.Select for use Stem and leaf of Radix Ginseng to replace Radix Ginseng, not only drawn the tonification function of Radix Ginseng, more dispeled the dry property of Radix Ginseng, prevention and therapeutic effect are better; Herba Erigerontis has expelling cold and relieving exterior syndrome, relaxing muscles and tendons to promote blood circulation, and pain relieving, the effect of removing food stagnancy, to the reduction cerebral vascular resistance, the cerebral blood flow increasing amount, microcirculation improvement, the permeability that improves blood brain barrier has good result.
The collaborative Herba Erigerontis of useful Radix Ginseng or Stem and leaf of Radix Ginseng is used for the treatment of the medicine of cardiovascular and cerebrovascular disease in the prior art, for example: application number is that 200310111064.0 Chinese invention patent discloses a kind of pharmaceutical composition for the treatment of cardiovascular and cerebrovascular disease, is made up of breviscapine and stem and leaf of Radix Ginseng saponin; Application number is that 200710051044.7 Chinese invention patent application discloses a kind of Chinese medicine composition and preparation method thereof and medical usage, is made up of Radix Ginseng or Stem and leaf of Radix Ginseng and Herba Erigerontis; Application number is that 200710051043.2 and 200710051042.8 Chinese invention patent application discloses a kind of pharmaceutical composition respectively, is made up of Radix Ginseng or extract of Radix Ginseng total saponins, Herba Erigerontis total flavones extract and Herba Erigerontis caffeic acid esters's extract; Application number is that 200710077834.2 Chinese invention patent application discloses a kind of pharmaceutical composition for the treatment of cardiovascular and cerebrovascular disease, comprises ginsenoside and breviscapine.Though said medicine has certain curative effect for the treatment treating cardiac and cerebral vascular diseases, effect is still obvious inadequately, and need adhere to for a long time taking, and takes effect slowly.Only Radix Ginseng and Herba Erigerontis are share and are difficult to further improve again curative effect, if but add too much medicine, not only can increase cost, in conjunction with after effect also may not be better than the additive effect of drug alone.
Summary of the invention
One of purpose of the present invention is the Chinese medicine composition that a kind of curative effect is good, preparation is simple, with low cost, safe and reliable is provided, and said composition has good preventing and therapeutic effect to cardiovascular and cerebrovascular disease.
Another object of the present invention is to provide a kind of preparation method of above-mentioned Chinese medicine composition.
Another purpose of the present invention is to provide the medical usage of above-mentioned Chinese medicine composition.
Chinese medicine composition of the present invention comprises that following raw medicaments in portion by weight makes: 2～18 parts of Herba Erigerontiss, Radix Ginseng or 1～12 part of Stem and leaf of Radix Ginseng, 2～18 parts of Radix Salviae Miltiorrhizaes.
More preferably, comprise that following raw medicaments in portion by weight makes: Herba Erigerontis 6-15 part, Radix Ginseng or Stem and leaf of Radix Ginseng 1-3 part, Radix Salviae Miltiorrhizae 6-15 part.
Optimally, comprise that following raw medicaments in portion by weight makes: Herba Erigerontis 8.5-15 part, Radix Ginseng or 1 part of Stem and leaf of Radix Ginseng, Radix Salviae Miltiorrhizae 8.5-15 part.
Chinese medicine composition of the present invention can be prepared as any dosage form of the prior art, is the best to be prepared into tablet, capsule, granule, injection, pill, drop pill, oral liquid.
The preparation method of Chinese medicine composition of the present invention comprises the steps:
(1) method of extraction Radix Ginseng or Stem and leaf of Radix Ginseng is as follows:
1. Radix Ginseng or the Stem and leaf of Radix Ginseng of getting described weight portion are ground into coarse powder, add alcohol reflux, filter, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
2. add water in the gained medicinal residues in 1. and decoct, filter, get filtrate II, filtrate II and filtrate I are merged, concentrating under reduced pressure becomes water extract;
3. the water extract after concentrating in will be is 2. put cold back and is added ethanol, and low temperature is placed, and filters, and filtrate recycling ethanol adds water again, and low temperature is placed, and filters, and filtrate is condensed into extractum, promptly gets Radix Ginseng or extract of Radix Ginseng stem and leaf;
(2) method of extraction Herba Erigerontis is as follows:
1. the Herba Erigerontis of getting described weight portion is ground into coarse powder, adds the aqueous slkali percolation and extracts, and collects percolate;
2. the 0.5-2 that the gained percolate is concentrated into Herba Erigerontis coarse powder weight in will be 1. doubly, pH value transfers to 5.0-7.0, leaves standstill, and gets supernatant, Herba Erigerontis extract;
(3) method of extraction Radix Salviae Miltiorrhizae is as follows:
1. the Radix Salviae Miltiorrhizae powder of getting described weight portion is broken into fine powder, adds ethanol and carries out the reflux, extract, filtration, and filtrate recycling ethanol also is condensed into clear paste I, and medicinal residues device are in addition collected;
2. add water in the gained medicinal residues in 1. and decoct, collecting decoction filters, and filtrate decompression is concentrated into clear paste II, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) Radix Ginseng or extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps is made makes required dosage form according to the conventional preparation method of each dosage form.
More preferably, comprise the steps:
(1) method of extraction Radix Ginseng or Stem and leaf of Radix Ginseng is as follows:
1. get the Radix Ginseng of described weight portion or the coarse powder that Stem and leaf of Radix Ginseng was ground into the 24-65 mesh sieve, add 8-10 and doubly measure the 60-75% alcohol reflux 3-5 time, each 30-60 minute, filter, it is standby to get filtrate I, and medicinal residues device are in addition collected;
2. in 1., add 2-4 times of water gaging in the gained medicinal residues and decoct 2-3 time, each 1-2 hour, filter, get filtrate II, filtrate II and filtrate I are merged, becoming relative density in 50-90 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
3. 2. middle gained water extract is put the ethanol that cold back adding 3-5 doubly measures 75-95%, low temperature was placed 36-60 hour, filter, filtrate recycling ethanol, add 2-4 times of water gaging again, low temperature was placed 18-36 hour, filtered, filtrate is condensed into the extractum that relative density is 1.1-1.2, gets Radix Ginseng or extract of Radix Ginseng stem and leaf;
(2) method of extraction Herba Erigerontis is as follows:
1. the Herba Erigerontis of getting described weight portion was ground into the coarse powder of 24-65 mesh sieve, added aqueous slkali soaking after 1-5 hour, with the speed percolation extraction of 5-20ml/min.kg, collected the percolate that Herba Erigerontis coarse powder 5-15 doubly measures;
2. the 0.5-2 that the gained percolate is concentrated into Herba Erigerontis coarse powder weight in will be 1. doubly, pH value transfers to 5.5-6.5, leaves standstill 6-24 hour, gets supernatant, Herba Erigerontis extract;
(3) method of extraction Radix Salviae Miltiorrhizae is as follows:
1. the Radix Salviae Miltiorrhizae powder of getting described weight portion was broken into the fine powder of 80-100 mesh sieve, added 8-10 and doubly measured the 75-95% alcohol reflux 2-3 time, each 1-2 hour, filtered filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device collection in addition;
2. add 2-4 times of water gaging in the gained medicinal residues and decoct 2-3 time in 1., each 1-2 hour, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, with clear paste I and clear paste II merging, gets Radix Salviae Miltiorrhizae extract;
(4) Radix Ginseng or extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps is made makes required dosage form according to the conventional preparation method of each dosage form.
Aqueous slkali in the described step (2) is selected from the NaCO that weight percent concentration is 1-5%
3The NaHCO of solution, 0.4-15%
3The NaOH solution of solution, 1-10%.
The application of pharmaceutical composition of the present invention aspect preparation prevention and treatment cardiovascular and cerebrovascular diseases medicament is used for prevention and diseases such as treatment cerebral infarction, cerebral ischemia, coronary heart disease, angina pectoris, myocardial ischemia, myocardial infarction, heart failure or arrhythmia.
Effects such as Radix Salviae Miltiorrhizae has stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, the relieving restlessness that clears away heart-fire.Compared with prior art, beneficial effect of the present invention is: the present invention share Radix Salviae Miltiorrhizae and Radix Ginseng or Stem and leaf of Radix Ginseng, Herba Erigerontis, is different from the existing single effect of medicine, simply drug combination.For treatment treating cardiac and cerebral vascular diseases disease significant curative effect is arranged, produced cooperative effect, obviously be better than Radix Ginseng or Stem and leaf of Radix Ginseng and Herba Erigerontis and share, improved clinical efficacy, have no side effect, safety is good.And preparation method of the present invention saves production cost than the easier realization of prior art, and utilization ratio of drug is higher, and curative effect is better.
Below further specify the drug action of pharmaceutical composition of the present invention by pharmacodynamics test:
According to " study of tcm new drug guide ", the pharmacodynamics of compositions of the present invention comprises the following aspects: observed the influence that the pharmaceutical composition under the different proportioning situations forms the dissolving of clotting time of mice, rats in vitro blood clot, rat thrombus in vivo.
Test method and result:
One, the present composition and compositions related different proportioning are to the influence of white mice clotting time
Get 130 of white mice, random packet, every group 10, clotting time of mice is measured the blood capillary method that adopts, blood capillary is (interior through 1mm, long 10cm), each treated animal gastric infusion is after 1 hour, cuts off mousetail (terminal 0.5cm place) and extrudes 2 and bleed on sheet glass, blood sucked in the blood capillary glass tubing immediately, begin to clock after lying against desktop, the end 0.5cm capillaceous that fractureed every 30 seconds after two minutes, and slowly draw back to the left and right, whether observe the place of fractureing has the blood clotting silk, till the appearance of blood clotting silk, between lasting, institute is clotting time.The results are shown in Table 1:
Table 1 present composition and compositions related different proportioning are to the influence of white mice clotting time
Annotate: compare with normal saline group matched group: * P＜0.05, * * P＜0.01.
The result shows: I-III energy significant prolongation clotting time of mice (* P＜0.05); Can prolong clotting time of mice (* * P＜0.01) for IV-VI number very significantly; And ii proportioning prescription obviously is better than i proportioning prescription.
Two, the present composition and compositions related different proportioning are to the dissolution of rat extracorporeal blood grumeleuse
After rat is weighed, chloralose with 13.3% urethane and 0.5%, dosage by 0.6ml/100gb.wt. is anaesthetized, row one side common carotid artery intubate, extract the non-anticoagulant arterial blood of 0.6ml, respectively get 0.3ml and inject two centrifuge tubes rapidly, treat carefully to take out behind its clot that congeals into, blot the liquid part of being infected with, use scales/electronic balance weighing, blood clot is reloaded two centrifuge tubes, by grouping, add relative medicine 1ml, matched group only adds the equivalent normal saline, and room temperature leaves standstill after 24 hours and takes out, blots the liquid part of being infected with, use scales/electronic balance weighing, relatively the weight of blood clot before and after the administration.The results are shown in Table 2:
Table 2 present composition and compositions related different proportioning compare the dissolution of rat extracorporeal blood grumeleuse
Annotate: compare with normal saline group matched group: * P＜0.05, * * P＜0.01.
The result shows that the different proportionings of the present composition have tangible dissolution to sludged blood; And ii proportioning prescription obviously is better than i proportioning prescription.
Three, the present composition and compositions related different proportioning are to the influence of rat thrombus in vivo formation
The silk thread of will performing the operation soaks the back with scales/electronic balance weighing and record with normal saline, and silk thread is penetrated in the polyethylene tube, and 1cm is respectively stayed at two ends, and with the perfusion of 60u/ml heparin, it is standby to make intubate, gets 130 of the SD rats that 300-400 restrains, and is divided into 13 groups at random, 10 every group.Give rat oral gavage according to dose (1ml/100g b.wt), irritate behind the stomach chloralose intraperitoneal injection of anesthesia with 13.3%.Separate rat one side common carotid artery and external jugular vein, note external the end of a thread, and keep the bulldog clamp closed condition.After waiting to irritate stomach 1h, open bulldog clamp, allow blood flow naturally by intubate, form arteriovenous shunt, folder closes common carotid artery behind the 15min, takes out silk thread and blood clot in the intubate rapidly, blots the back and uses scales/electronic balance weighing, deducts the silk thread weight in wet base, is wet weight of thrombus.The results are shown in Table 3:
The influence that table 3 present composition and compositions related different proportioning form the rat thrombus in vivo
Annotate: compare with normal saline group matched group: * P＜0.05, * * P＜0.01.
The result shows that the different proportionings of the present composition all have the obvious suppression effect to the formation of thrombus in vivo, compare with normal saline, and thrombus weight obviously alleviates; And ii proportioning prescription obviously is better than i proportioning prescription.
The specific embodiment
Below in conjunction with embodiment Chinese medicine composition of the present invention and preparation method thereof is described in further detail.
Embodiment 1 tablet
The present composition is made by following raw material medicaments: Stem and leaf of Radix Ginseng 12g, Herba Erigerontis 2g, Radix Salviae Miltiorrhizae 2g.
The preparation method of the present composition (tablet) is:
(1) preparation extract of Radix Ginseng stem and leaf:
Get the coarse powder that Stem and leaf of Radix Ginseng 12g was ground into 65 mesh sieves, adding coarse powder weight 10 times of amounts, concentration is 75% alcohol reflux 5 times, each 30 minutes, filter, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
In medicinal residues, add 3 times of water gagings of medicinal residues weight and decoct 3 times, each 1 hour, filter, get filtrate II, filtrate II and filtrate I are merged, becoming relative density in 90 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 5 times of amounts, concentration be 95% ethanol, and low temperature was placed 60 hours, filtration, filtrate recycling ethanol adds 4 times of water gagings again, and low temperature was placed 36 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get extract of Radix Ginseng stem and leaf;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 2g was ground into 65 mesh sieves, add 5% NaCO
3After the solution soaking 1 hour,, collect the percolate of 5 times of amounts of Herba Erigerontis coarse powder with the speed percolation extraction of 20ml/min.kg;
Percolate is concentrated into 0.5 times of Herba Erigerontis coarse powder weight, and pH value transfers to 5.5, leaves standstill 6 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 2g was ground into 100 mesh sieves, add 10 times of amount 95% alcohol reflux 3 times, each 1 hour, filter, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device are in addition collected;
Add 3 times of water gagings and decoct 3 times in medicinal residues, each 1 hour, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) make tablet:
With extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps makes, add the starch of three kinds of extract total amounts 10%, the back tabletting of granulating is made the tablet of 0.5g/ sheet, labels, check, promptly.
Embodiment 2 capsules
The present composition is made by following raw material medicaments: Radix Ginseng 3g, Herba Erigerontis 6g, Radix Salviae Miltiorrhizae 6g.
The preparation method of the present composition (capsule) is:
(1) preparation Radix Ginseng extract:
Get the coarse powder that Radix Ginseng 3g was ground into 24 mesh sieves, adding coarse powder weight 10 times of amounts, concentration is 70% alcohol reflux 3 times, each 60 minutes, filter, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
Adding water in medicinal residues decocts 2 times: add 4 times of water gagings of medicinal residues weight for the first time, decocted 2 hours, add for the second time 3 times of water gagings of medicinal residues weight, decocted 1 hour, collecting decoction filters, and gets filtrate II, filtrate II and filtrate I are merged, and becoming relative density in 80 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 4 times of amounts, concentration be 85% ethanol, and low temperature was placed 48 hours, filtration, filtrate recycling ethanol adds 4 times of water gagings again, and low temperature was placed 36 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get Radix Ginseng extract;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 6g was ground into 24 mesh sieves, add 1% NaCO
3After the solution soaking 5 hours,, collect the percolate of 5 times of amounts of Herba Erigerontis coarse powder with the speed percolation extraction of 20ml/min.kg;
Percolate is concentrated into 2 times of Herba Erigerontis coarse powder weight, and pH value transfers to 7.0, leaves standstill 24 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 6g was ground into 80 mesh sieves, add 10 times of amount 95% alcohol reflux 2 times, each 1 hour, filter, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device are in addition collected;
Add 4 times of water gagings and decoct 2 times in medicinal residues, each 1 hour, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) make capsule:
With Radix Ginseng extract, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) step makes, add the starch of three kinds of extract total amounts 30%, granulate, drying, encapsulated, make capsule, check, promptly.
Embodiment 3 granules
The present composition is made by following raw material medicaments: Stem and leaf of Radix Ginseng 1g, Herba Erigerontis 8.5g, Radix Salviae Miltiorrhizae 8.5g.
The preparation method of the present composition (granule) is:
(1) preparation extract of Radix Ginseng stem and leaf:
Get the coarse powder that Stem and leaf of Radix Ginseng 1g was ground into 50 mesh sieves, adding coarse powder weight 8 times of amounts, concentration is 60% alcohol reflux 3 times, and each 30 minutes, collecting decoction filtered, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
Add 3 times of water gagings of medicinal residues weight and decoct 2 times in medicinal residues, each 1 hour, merging filtrate filtered, and gets filtrate II, and filtrate II and filtrate I are merged, and becoming relative density in 60 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 4 times of amounts, concentration be 85% ethanol, and low temperature was placed 48 hours, filtration, filtrate recycling ethanol adds 3 times of water gagings again, and low temperature was placed 24 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get extract of Radix Ginseng stem and leaf;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 8.5g was ground into 50 mesh sieves, the NaOH solution soaking of adding 10% with the speed percolation extraction of 10ml/min.kg, was collected the percolate of 10 times of amounts of Herba Erigerontis coarse powder after 3 hours;
Percolate is concentrated into and weight such as Herba Erigerontis coarse powder, and pH value transfers to 6.5, leaves standstill 12 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 8.5g was ground into 100 mesh sieves, add 10 times of amount 85% alcohol reflux 2 times, each 1 hour, filter, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device are in addition collected;
Add 3 times of water gagings of medicinal residues weight and decoct 2 times in medicinal residues, each 1 hour, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) make granule:
Extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract mixing with (1)-(3) steps makes add three kinds of extract total amount 50% dextrin and 20% sucrose, granulate, and drying is dressed up the granule of 3g/ bag, labels, and checks, promptly.
Embodiment 4 liquid drugs injections
The present composition is made by following raw material medicaments: Radix Ginseng 1g, Herba Erigerontis 10g, Radix Salviae Miltiorrhizae 10g.
The preparation method of the present composition (liquid drugs injection) is:
(1) preparation Radix Ginseng extract:
Get the coarse powder that Radix Ginseng 1g was ground into 65 mesh sieves, adding coarse powder weight 8 times of amounts, concentration is 60% alcohol reflux 4 times, and each 30 minutes, collecting decoction filtered, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
Adding water in medicinal residues decocts 3 times: add 4 times of water gagings of medicinal residues weight for the first time, decocted 1 hour, for the second time add 3 times of water gagings of medicinal residues weight, decocted 1 hour, add 2 times of water gagings of medicinal residues weight for the third time, decocted 1 hour, merging filtrate filters, and gets filtrate II, filtrate II and filtrate I are merged, and becoming relative density in 50 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 3 times of amounts, concentration be 75% ethanol, and low temperature was placed 36 hours, filtration, filtrate recycling ethanol adds 2 times of water gagings again, and low temperature was placed 18 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get Radix Ginseng extract;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 10g was ground into 50 mesh sieves, add 0.4% NaHCO
3After the solution soaking 1 hour,, collect the percolate of 5 times of amounts of Herba Erigerontis coarse powder with the speed percolation extraction of 5ml/min.kg;
Percolate is concentrated into 1.5 times of Herba Erigerontis coarse powder weight, and pH value transfers to 6, leaves standstill 12 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 10g was ground into 80 mesh sieves, add 8 times of amount 85% alcohol reflux 3 times, each 1 hour, filter, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device are in addition collected;
In medicinal residues, decoct with water 3 times, for the first time add 4 times of water gagings of medicinal residues weight, decocted 2 hours, add 3 times of water gagings of medicinal residues weight for the second time, decocted 1 hour, add 2 times of water gagings of medicinal residues weight for the third time, decocted collecting decoction 1 hour, filter, it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, clear paste I and clear paste II are merged, Radix Salviae Miltiorrhizae extract;
(4) make liquid drugs injection:
Get the Herba Erigerontis extract of above-mentioned (2) gained, add about 45 times of amount water for injection suspendibles after, with the about 7-8 of 0.3mol/L sodium hydroxide adjust pH, make the extract dissolving clear and bright, must Herba Erigerontis extract solution; Get the Radix Salviae Miltiorrhizae extract of above-mentioned (3) gained, add about 45 times of amount water for injection suspendibles after, with the about 7-8 of 0.3mol/L sodium hydroxide adjust pH, make the extract dissolving clear and bright, must Radix Salviae Miltiorrhizae extract solution; Get the Radix Ginseng extract of above-mentioned (1) gained again, add the dissolving of about 12 times of amount water for injection, get Radix Ginseng extract solution, three kinds of extract solution mixings;
Other gets the sodium pyrosulfite that is equivalent to Radix Ginseng extract, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract total amount 85%, with the dissolving of about 6 times of amount water for injection, with said extracted thing solution mixing, transfer pH to 6.5-7.0 with the 0.3mol/L sodium hydroxide, press 0.3g/100ml and add active carbon, add 10% ethanol to total extract concentration again and be about 2.5g/100ml, stirred 10 minutes, filter with the microporous filter membrane of 0.45 μ m, 0.22 μ m respectively, filtrate is propped up the branch ampoule of packing into, sealing by fusing by 5ml/, 115 ℃ of pressure sterilizings 30 minutes, label, check, promptly.
Radix Ginseng extract, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract mixing with (1)-(3) steps makes add three kinds of extract total amount 50% dextrin and 20% sucrose, granulate, and drying is dressed up the granule of 3g/ bag, labels, and checks, promptly.
Embodiment 5 freeze-dried powders
The present composition is made by following raw material medicaments: Stem and leaf of Radix Ginseng 1g, Herba Erigerontis 15g, Radix Salviae Miltiorrhizae 15g.
The preparation method of the present composition (freeze-dried powder) is:
(1) preparation extract of Radix Ginseng stem and leaf:
Get the coarse powder that Stem and leaf of Radix Ginseng 1g was ground into 50 mesh sieves, adding coarse powder weight 10 times of amounts, concentration is 75% alcohol reflux 4 times, and each 60 minutes, collecting decoction filtered, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
Add 3 times of water gagings of medicinal residues weight and decoct 3 times in medicinal residues, each 1 hour, merging filtrate filtered, and gets filtrate II, and filtrate II and filtrate I are merged, and becoming relative density in 90 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 5 times of amounts, concentration be 95% ethanol, and low temperature was placed 60 hours, filtration, filtrate recycling ethanol adds 4 times of water gagings again, and low temperature was placed 36 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get extract of Radix Ginseng stem and leaf;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 15g was ground into 24 mesh sieves, add 15% NaHCO
3After the solution soaking 1 hour,, collect the percolate of 15 times of amounts of Herba Erigerontis coarse powder with the speed percolation extraction of 20ml/min.kg;
Percolate is concentrated into 0.8 times of Herba Erigerontis coarse powder weight, and pH value transfers to 5.0, leaves standstill 18 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 15g was ground into 100 mesh sieves, add 10 times of amount 95% alcohol reflux 2 times, each 1 hour, filter, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device are in addition collected;
Add 3 times of water gagings of medicinal residues weight and decoct 2 times in medicinal residues, each 2 hours, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) make freeze-dried powder:
Get the Herba Erigerontis extract of above-mentioned (2) gained, add about 60 times of amount water for injection suspendibles after, with the about 7-7.5 of 1mol/L sodium hydroxide adjust pH, make the extract dissolving clear and bright, must Herba Erigerontis extract solution; Get the Radix Salviae Miltiorrhizae extract of above-mentioned (3) gained, add about 60 times of amount water for injection suspendibles after, with the about 7-7.5 of 1mol/L sodium hydroxide adjust pH, make the extract dissolving clear and bright, must Radix Salviae Miltiorrhizae extract solution; Get the extract of Radix Ginseng stem and leaf of above-mentioned (1) gained again, add the dissolving of about 15 times of amount water for injection, get extract of Radix Ginseng stem and leaf solution, three kinds of extract solution mixings;
Other gets the mannitol that is equivalent to 10 times of extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract total amounts, with the dissolving of about 4 times of amount water for injection, with said extracted thing solution mixing, add injection water to total extract concentration and be about 0.75g/100ml, press 0.4g/100ml again and add active carbon, stirred 15 minutes, microporous filter membrane with 0.45 μ m, 0.22 μ m filters respectively, filtrate is propped up the branch cillin bottle of packing into by 5ml/, place lyophilization in the freeze dryer, add a cover butyl rubber plug and aluminium lid, label, check, promptly.
Embodiment 6 pills
The present composition is made by following raw material medicaments: Stem and leaf of Radix Ginseng 1g, Herba Erigerontis 18g, Radix Salviae Miltiorrhizae 18g.
The preparation method of the present composition (pill) is:
(1) preparation extract of Radix Ginseng stem and leaf:
Get the coarse powder that Stem and leaf of Radix Ginseng 1g was ground into 24 mesh sieves, adding coarse powder weight 9 times of amounts, concentration is 70% alcohol reflux 5 times, and each 60 minutes, collecting decoction filtered, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
3 times of water gagings of adding medicinal residues weight decoct 2 times in medicinal residues, and 2 hours for the first time, 1 hour for the second time, merging filtrate filtered, and gets filtrate II, and filtrate II and filtrate I are merged, and becoming relative density in 60 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
Water extract is put cold back, and to add water extract weight 4 times of amounts, concentration be 85% ethanol, and low temperature was placed 60 hours, filtration, filtrate recycling ethanol adds 3 times of water gagings again, and low temperature was placed 36 hours, filter, filtrate is condensed into the extractum that relative density is 1.1-1.2, get extract of Radix Ginseng stem and leaf;
(2) preparation Herba Erigerontis extract:
Get the coarse powder that Herba Erigerontis 18g was ground into 50 mesh sieves, the NaOH solution soaking of adding 1% with the speed percolation extraction of 10ml/min.kg, was collected the percolate of 10 times of amounts of Herba Erigerontis coarse powder after 3 hours;
Percolate is concentrated into 1.2 times of Herba Erigerontis coarse powder weight, and pH value transfers to 6.0, leaves standstill 18 hours, gets supernatant, Herba Erigerontis extract;
(3) preparation Radix Salviae Miltiorrhizae extract:
Get the fine powder that Radix Salviae Miltiorrhizae 18g was ground into 100 mesh sieves, add 10 times of amount 75% alcohol reflux 3 times, 2 hours each time, second and third time 1 hour filtered, filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, and medicinal residues device are in addition collected;
In medicinal residues, add 3 times of water gagings of medicinal residues weight and decoct 3 times, 2 hours for the first time, second and third time 1 hour, collecting decoction filters, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) make pill:
Extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps is made, the refined honey that adds three kinds of extract total amounts 80% be as binding agent, mixing, and pill, drying is made honeyed pill, labels, check, promptly.
Embodiment 7 drop pills
This routine described crude drug and preparation method have different being only substantially with embodiment 3:
(4) make drop pill:
With extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract mixing that (1)-(3) step makes, spray drying is ground into fine powder, sieves, and is standby; Taking polyethylene glycol 4000 is heated to 80-90 ℃ and makes moltenly, adds the fine powder after the above-mentioned spray drying, stirs fusion, and maintains the temperature at 85 ℃; Above-mentioned mixed liquor is poured in the drop pill device, and 85 ℃ of water bath heat preservations splash in 5 ℃ the liquid paraffin, after the system of dripping finishes drop pill are continued to stay in the liquid coolant, and it is fully cooled off, and take out, and inhale and remove surface liquid paraffin, drying, promptly.
Embodiment 8 oral liquids
This routine described crude drug and preparation method have different being only substantially with embodiment 4:
(4) make oral liquid:
With Radix Ginseng extract, Herba Erigerontis extract and Radix Salviae Miltiorrhizae extract mixing that (1)-(3) step makes, adding water to relative density is 1.1-1.2, and mixing pours in the medicinal high-density polyethylene bottle of oral administration solid, promptly gets oral liquid.
Claims (9)
1. Chinese medicine composition for the treatment of cardiovascular and cerebrovascular disease is characterized in that comprising that following raw medicaments in portion by weight makes: 2～18 parts of Herba Erigerontiss, Radix Ginseng or 1～12 part of Stem and leaf of Radix Ginseng, 2～18 parts of Radix Salviae Miltiorrhizaes.
2. Chinese medicine composition as claimed in claim 1 is characterized in that comprising that following raw medicaments in portion by weight makes: Herba Erigerontis 6-15 part, Radix Ginseng or Stem and leaf of Radix Ginseng 1-3 part, Radix Salviae Miltiorrhizae 6-15 part.
3. Chinese medicine composition as claimed in claim 1 is characterized in that comprising that following raw medicaments in portion by weight makes: Herba Erigerontis 8.5-15 part, Radix Ginseng or 1 part of Stem and leaf of Radix Ginseng, Radix Salviae Miltiorrhizae 8.5-15 part.
4. as the arbitrary described Chinese medicine composition of claim 1-3, it is characterized in that described preparation of compositions is any dosage form of the prior art.
5. Chinese medicine composition as claimed in claim 4 is characterized in that described preparation of compositions is tablet, capsule, granule, pill, injection, drop pill, oral liquid.
6. the preparation method of a Chinese medicine composition as claimed in claim 1 comprises the steps:
(1) method of extraction Radix Ginseng or Stem and leaf of Radix Ginseng is as follows:
1. Radix Ginseng or the Stem and leaf of Radix Ginseng of getting described weight portion are ground into coarse powder, add alcohol reflux, filter, and it is standby to get filtrate I, and medicinal residues device are in addition collected;
2. add water in the gained medicinal residues in 1. and decoct, filter, get filtrate II, filtrate II and filtrate I are merged, concentrating under reduced pressure becomes water extract;
3. 2. middle gained water extract is put cold back adding ethanol, low temperature is placed, and filters, and filtrate recycling ethanol adds water again, and the low temperature placement filters, and filtrate is condensed into extractum, promptly gets Radix Ginseng or extract of Radix Ginseng stem and leaf;
(2) method of extraction Herba Erigerontis is as follows:
1. the Herba Erigerontis of getting described weight portion is ground into coarse powder, adds the aqueous slkali percolation and extracts, and collects percolate;
2. the 0.5-2 that the gained percolate is concentrated into Herba Erigerontis coarse powder weight in will be 1. doubly, pH value transfers to 5.0-7.0, leaves standstill, and gets supernatant, Herba Erigerontis extract;
(3) method of extraction Radix Salviae Miltiorrhizae is as follows:
1. the Radix Salviae Miltiorrhizae powder of getting described weight portion is broken into fine powder, adds ethanol and carries out the reflux, extract, filtration, and filtrate recycling ethanol also is condensed into clear paste I, and medicinal residues device are in addition collected;
2. add water in the gained medicinal residues in 1. and decoct, collecting decoction filters, and filtrate decompression is concentrated into clear paste II, and clear paste I and clear paste II are merged, and gets Radix Salviae Miltiorrhizae extract;
(4) Radix Ginseng or extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps is made makes required dosage form according to the conventional preparation method of each dosage form.
7. the preparation method of Chinese medicine composition as claimed in claim 6 comprises the steps:
(1) method of extraction Radix Ginseng or Stem and leaf of Radix Ginseng is as follows:
1. get the Radix Ginseng of described weight portion or the coarse powder that Stem and leaf of Radix Ginseng was ground into the 24-65 mesh sieve, add 8-10 and doubly measure the 60-75% alcohol reflux 3-5 time, each 30-60 minute, filter, it is standby to get filtrate I, and medicinal residues device are in addition collected;
2. in 1., add 2-4 times of water gaging in the gained medicinal residues and decoct 2-3 time, each 1-2 hour, filter, get filtrate II, filtrate II and filtrate I are merged, becoming relative density in 50-90 ℃ of concentrating under reduced pressure is the water extract of 1.1-1.2;
3. 2. middle gained water extract is put the ethanol that cold back adding 3-5 doubly measures 75-95%, low temperature was placed 36-60 hour, filter, filtrate recycling ethanol, add 2-4 times of water gaging again, low temperature was placed 18-36 hour, filtered, filtrate is condensed into the extractum that relative density is 1.1-1.2, gets Radix Ginseng or extract of Radix Ginseng stem and leaf;
(2) method of extraction Herba Erigerontis is as follows:
1. the Herba Erigerontis of getting described weight portion was ground into the coarse powder of 24-65 mesh sieve, added aqueous slkali soaking after 1-5 hour, with the speed percolation extraction of 5-20ml/min.kg, collected the percolate that Herba Erigerontis coarse powder 5-15 doubly measures;
2. the 0.5-2 that the gained percolate is concentrated into Herba Erigerontis coarse powder weight in will be 1. doubly, pH value transfers to 5.5-6.5, leaves standstill 6-24 hour, gets supernatant, Herba Erigerontis extract;
(3) method of extraction Radix Salviae Miltiorrhizae is as follows:
1. the Radix Salviae Miltiorrhizae powder of getting described weight portion was broken into the fine powder of 80-100 mesh sieve, added 8-10 and doubly measured the 75-95% alcohol reflux 2-3 time, each 1-2 hour, filtered filtrate recycling ethanol and to be condensed into relative density be 1.36 clear paste I, medicinal residues device collection in addition;
2. add 2-4 times of water gaging in the gained medicinal residues and decoct 2-3 time in 1., each 1-2 hour, collecting decoction filtered, and it is 1.36 clear paste II that filtrate decompression is concentrated into relative density, with clear paste I and clear paste II merging, gets Radix Salviae Miltiorrhizae extract;
(4) Radix Ginseng or extract of Radix Ginseng stem and leaf, Herba Erigerontis extract and the Radix Salviae Miltiorrhizae extract mixing that (1)-(3) steps is made makes required dosage form according to the conventional preparation method of each dosage form.
8. as the preparation method of claim 6 or 7 described Chinese medicine compositions, it is characterized in that: the aqueous slkali in the described step (2) is selected from the NaCO that weight percent concentration is 1-5%
3The NaHCO of solution, 0.4-15%
3The NaOH solution of solution, 1-10%.
As the arbitrary described Chinese medicine composition of claim 1-3 in the application aspect preparation prevention and the treatment cardiovascular and cerebrovascular diseases medicament.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435106A (en) * | 2014-11-10 | 2015-03-25 | 成都果睿医药科技有限公司 | Medicine composition containing salviae miltiorrhizae for treating cardiovascular and cerebrovascular diseases |
| CN107441163A (en) * | 2017-08-04 | 2017-12-08 | 上海华源安徽锦辉制药有限公司 | A kind of preparation method for improving red sage root dropping liquid stability |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961917A (en) * | 2005-11-11 | 2007-05-16 | 北京奇源益德药物研究所 | Pharmaceutical composition for treating cardiovascular and cerebrovascular diseases, preparation method and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961917A (en) * | 2005-11-11 | 2007-05-16 | 北京奇源益德药物研究所 | Pharmaceutical composition for treating cardiovascular and cerebrovascular diseases, preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435106A (en) * | 2014-11-10 | 2015-03-25 | 成都果睿医药科技有限公司 | Medicine composition containing salviae miltiorrhizae for treating cardiovascular and cerebrovascular diseases |
| CN107441163A (en) * | 2017-08-04 | 2017-12-08 | 上海华源安徽锦辉制药有限公司 | A kind of preparation method for improving red sage root dropping liquid stability |
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