CN101880712B - Preparation method of epoxy group modified bio-chip substrate - Google Patents
Preparation method of epoxy group modified bio-chip substrate Download PDFInfo
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- CN101880712B CN101880712B CN 201010167800 CN201010167800A CN101880712B CN 101880712 B CN101880712 B CN 101880712B CN 201010167800 CN201010167800 CN 201010167800 CN 201010167800 A CN201010167800 A CN 201010167800A CN 101880712 B CN101880712 B CN 101880712B
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Abstract
The invention relates to a preparation method of an epoxy group modified bio-chip substrate, which comprises the following steps: (1) immersing a high borosilicate glass sheet after ultrasonic processing in miscible liquids of concentrated sulfuric acid, hydrogen peroxide and ultra-pure water, leaving the high borosilicate glass sheet in the miscible liquids under the temperature of 80-130 DEG C for 15-30 minutes, then immersing the high borosilicate glass sheet in miscible liquids of concentrated hydrochloric acid and alcohol after cooling and washing, and cleaning and drying the high borosilicate glass sheet; (2) preparing absolute ethyl alcohol containing GPTMS and glacial acetic acid or isopropanol solution, magetic stirring, raising the temperature to 60-80 DEG C, and back flowing; (3) adjusting PH of silane coupling agent solution to be 7, and stirring; and adding the high borosilicate glass sheet after surface treatment immediately, laying aside, cleaning, and drying. The method of the invention has the advantages of simple process and low cost, and is applicable for industrial production; the obtained substrate has the advantages of low background, uniform surface, regular sampling point and strong bonding force, and increases the fixing efficiency significantly. Moreover, the substrate has high capabilities of loading and protein molecule combination and is a novel protein micro-array carrier.
Description
Technical field
The invention belongs to the preparation field of bio-chip substrate, particularly relate to a kind of preparation method of epoxy group modified bio-chip substrate.
Background technology
Biochip technology has begun to be widely used in aspects such as genomics research, single nucleotide polymorphism analysis, clinical medical inspection as a kind of important tool that can carry out the various article parallel analysis of high-throughput.It is arranged in a large amount of biomacromolecule probe, gene fragment, oligonucleotide or protein etc. on the fixed position of special carrier by ad hoc fashion; Under given conditions with sample effect to be checked; Scanner through precision writes down, detects, analyzes, and has advantages such as level of automation height, testing goal molecular amounts are more, high-throughput.
In general biochip selects silicon chip, sheet glass, tinsel, plastic sheet or polypropylene screen, the nitrocellulose filter etc. of process handled as carrier.Sheet glass has been widely used in the making of DNA and protein chip microarray owing to have cheapness, smooth surface, low fluorescence background and steady performance.Make probe stationary in glass sheet surface, must carry out physical chemistry modifying to glass sheet surface earlier.The method of glass sheet surface chemically modified at present is a lot, is broadly divided into two-dimensional surface modification and three-dimensional surface and modifies two types.
With the sheet glass to be that the two-dimensional surface of carrier is modified with amido modified, aldehyde group modified, sulfydryl modification and polylysine modification etc.; This type carrier most of through some intermolecular avidity or covalent linkage gene or albumen probe stationary on its surface, this method cost is lower, be convenient to preparation, good reproducibility, fluorescence background are relatively low.But still have certain defective, less like the probe stationary amount, uniform surface property is poor, point of sample is regular inadequately and surface functional group and probe between non-specific interaction takes place makes problems such as the sheet glass background increases.It is at glass sheet surface bag layer of gel or dendron shape polymer that three-dimensional surface is modified, and then introduces reactive group, thereby forms 3-D solid structure in glass sheet surface.Because three-dimensional porous structure, carrier surface and probe contact area increase greatly, thereby fixed amount also increases, and has improved fixed efficiency; In addition, moistening microenvironment makes the protein soln that is fixed in the above be difficult for evaporation in the gel, thereby has kept proteic activity.But because these nano level porous material structures are comparatively complicated, make distance control difficulty comparatively between various biomacromolecule probes on the microarray, therefore the interaction between probe and the porous material strengthens, and the background when causing detecting increases; In addition, the use of these macromole porous materials has also brought problem for the follow-up wash-out of microarray.Therefore there is the invention of the bio-chip substrate that good biomolecules binding ability and surface quality are high, background is low, strength of signal is high imperative.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation method of epoxy group modified bio-chip substrate, and this preparation method is simple, and cost is low, is suitable for suitability for industrialized production; Gained substrate background is low, uniform surface, point of sample is more regular, bonding force is strong, has improved fixed efficiency greatly, and it has the ability of high loads and conjugated protein molecule, is one type of newer protein microarray carrier.
The preparation method of a kind of epoxy group modified bio-chip substrate of the present invention comprises:
(1) high-boron-silicon glass sheet surface treatment: the high-boron-silicon glass sheet that supersound process is crossed is immersed in the mixed solution of the vitriol oil, hydrogen peroxide and ultrapure water (volume ratio is 4:1:20); 80～130 ℃ of held 15～30 minutes; Be soaked in after cooling, the ultrapure water flushing in concentrated hydrochloric acid and ethanol (volume ratio the is 1:1) mixing solutions 3～24 hours; Ultrapure water cleans 3～5 times, dries 15～30 minutes for 110～140 ℃;
(2) preparation of epoxy silane coupling solution: preparation contains the absolute ethyl alcohol or the aqueous isopropanol of 1～3vol%GPTMS, 1～3vol% Glacial acetic acid min. 99.5; Magnetic agitation is warming up to 60～80 ℃, refluxes 1～2 hour; Reduce to room temperature, whole process is carried out under the lucifuge condition;
(3) epoxy group modified: as to regulate the pH=7 of above-mentioned silane coupler solution, magnetic agitation 3～5 minutes; Add the high-boron-silicon glass sheet after surface treatment rapidly; At 25～30 ℃; Relative humidity is to place in 30～50% climatic chambers 2～24 hours, takes out the back and cleans with absolute ethyl alcohol earlier, and then wash with ultrapure water; Dried 15～30 minutes, and promptly got epoxy group modified bio-chip substrate for 110～140 ℃.
High-boron-silicon glass sheet physical dimension in the said step (1) is that 25.0mm ± 0.2mm * 75.6mm ± 0.2mm, thickness are 1.0mm ± 0.02mm, it is advantageous that: " zero " fluorescence background, polished smooth degree (± 20 dust), the sheet glass differences coefficient (CV)＜10% of atom that reached in surface.
Ultrasonic in the said step (1) is in ultrapure water ultrasonic 5～10 minutes.
The preparation of epoxy silane coupling solution in the said step (2): get 99.8% absolute ethyl alcohol 77.6ml, GPTMS2.4ml, Glacial acetic acid min. 99.5 2.4ml joins in the there-necked flask, is heated to 60 ℃ with oil bath, magnetic agitation refluxed 2 hours, reduces to room temperature then.
The preparation of epoxy silane coupling solution in the said step (2): get 1mlGPTMS, the 98ml Virahol, 1ml acetic acid joins in the there-necked flask, is heated to 80 ℃ with oil bath, magnetic agitation refluxed 1 hour, reduces to room temperature then.
Use strong aqua to regulate the pH of silane coupler solution in the said step (3).
Beneficial effect
(1) preparation method of bio-chip substrate provided by the present invention is simple, not only can be used to prepare epoxy group modified bio-chip substrate, also can be used for the preparation of amido modified bio-chip substrate simultaneously;
(2) the prepared bio-chip substrate of the present invention; Because the activity of surperficial epoxy group(ing) is stronger; Improve substrate and combined the amido modified dna probe and the ability of albumen probe; Thereby reduced the point sample concentration of dna probe and albumen probe in preparation biochip process, finally reduced the cost of biochip, promoted further developing of biochip industry;
(3) more regular, the strength of signal of low, the uniform surface of the bio-chip substrate background of gained of the present invention, point of sample reaches about 60,000 far above common amino sheet, aldehyde radical sheet etc.; Not only solved common amino sheet and common aldehyde radical sheet amido modified nucleic probe has been fixed problem weak or that do not fix; Improved fixed efficiency greatly, and it having the ability of high loads and conjugated protein molecule, is one type of newer protein microarray carrier; Its surperficial epoxy group(ing) activity is very strong; Not only can react with amino, can also with reactions such as other groups of protein surface such as carboxyl, sulfydryl, hydroxyl, as illustrated in fig. 1 and 2; The degree of variation of its point of epoxy substrate of the present invention is less, does not have conditions of streaking, and point is more mellow and fuller, and background is lower, is the substrate that a kind of ideal prepares biochip;
(4) the bio-chip substrate surface of gained of the present invention has firm specificity binding site, can be widely used in the carrier of dna microarray and arrays of immobilized protein.
Description of drawings
The reaction mechanism figure of Fig. 1 epoxy group(ing) and amido modified dna molecular
The reaction mechanism figure of Fig. 2 epoxy group(ing) and protein surface reactive group
Point sample matrix synoptic diagram in the test of Fig. 3 epoxy substrate point sample
Fluorescent scanning picture behind Fig. 4 epoxy substrate point sample.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
The preparation of epoxy group(ing) bio-chip substrate
(1) measure 99.8% absolute ethyl alcohol 77.6ml respectively, GPTMS2.4ml, Glacial acetic acid min. 99.5 2.4ml joins in the there-necked flask, is heated to 60 ℃ with oil bath, magnetic agitation refluxed 2 hours, reduces to room temperature then, and whole process is carried out under the lucifuge condition.
(2) the borosilicate glass sheet was put into the staining jar that ultrapure water is housed ultrasonic 10 minutes; Twice of ultrapure water flushing; Be soaked in then in the mixed solution of the vitriol oil, hydrogen peroxide and ultrapure water (volume ratio is 4: 1: 20); In 130 ℃ air dry oven, placed 15 minutes, cooling is with ultrapure water flushing 3 times; In concentrated hydrochloric acid and ethanol (volume ratio is 1: 1) mixing solutions, soaked 3 hours then, ultrapure water cleans 3 times, dries 30 minutes, and is cooled to room temperature for 110 ℃.
(3) in the silane coupler solution for preparing, add strong aqua, regulator solution pH=7 stirred 5 minutes at lucifuge condition lower magnetic force; Surface treated borosilicate glass sheet is put into epoxy silane coupling solution, put into climatic chamber then, relative humidity is controlled at 50%; Temperature is set at 30 ℃, places respectively 2,5,8,10,15,20,24 hours, takes out the back with absolute ethyl alcohol flushing 3 times; Ultrapure water flushing 3 times, 110 ℃ of oven dry promptly obtained the epoxy group(ing) substrate in 30 minutes.
Resulting surface-treated high-boron-silicon glass sheet and epoxy group(ing) substrate have carried out surperficial sign with AFM respectively among this embodiment.
Embodiment 2
The preparation of epoxy group(ing) bio-chip substrate
(1) measure 1mlGPTMS respectively, the 98ml Virahol, 1ml acetic acid joins in the there-necked flask, is heated to 80 ℃ with oil bath, magnetic agitation refluxed 1 hour, reduces to room temperature then, and whole process is carried out under the lucifuge condition.
(2) at first the borosilicate glass sheet is put into staining jar at ultrapure water ultrasonic 5 minutes; Ultrapure water is soaked in the mixed solution of the vitriol oil, hydrogen peroxide and ultrapure water (volume ratio is 4: 1: 20) after washing 3 times; Placed 30 minutes for 80 ℃, cooling, ultrapure water cleans 3 times; Be soaked in then in concentrated hydrochloric acid and ethanol (volume ratio is 1: the 1) mixing solutions 24 hours, 140 ℃ of oven dry were 15 minutes after ultrapure water cleaned 5 times.
(3) in the silane coupler solution for preparing, add strong aqua, regulator solution pH=7 stirred 3 minutes at lucifuge condition lower magnetic force; Surface treated borosilicate glass sheet is put into epoxy silane coupling solution, put into climatic chamber then, relative humidity is controlled at 30%; Temperature is set at 25 ℃, places 2,5,8,15,20,24 hours, takes out the back and cleans 3 times with absolute ethyl alcohol; Ultrapure water cleans 3 times, and 140 ℃ of oven dry promptly obtained the epoxy substrate after 15 minutes.
Embodiment 3
The detection of epoxy group(ing) bio-chip substrate crystallized ability
The used epoxy group(ing) substrate of present embodiment is by embodiment 1 described method preparation; Present embodiment is through Arrayit SpotBot
2 point sample instruments the epoxy substrate to be carried out the point sample test; Point sample matrix design synoptic diagram is as shown in Figure 3; And then through Axon GenePix
4000B:Cy3PMT600; The Power100 scanner detects the crystallized ability that its fluorescence signal intensity reflects the epoxy substrate, and concrete operations are following:
(1) prepares the point sample sample
A. under the room temperature, dna probe is clipped to 50%DMSO/50%H with the concentration branch of 0.05 μ M, 0.1 μ M, 0.5 μ M, 1 μ M
2In the damping fluid of O.Antibody probe is clipped to 20% glycerine/80%H with the concentration branch of 0.5 μ g/ μ l, 1 μ g/ μ l, 2.5 μ g/ μ l, 5 μ g/ μ l
2In the damping fluid of O;
B. sample evidence matrix design synoptic diagram is transferred in 96 orifice plates or 384 orifice plates, rapped microwell plate and make at the bottom of liquid flow to the hole, slide neatly is placed on the dot matrix instrument chassis;
C. start dot matrix instrument, the array of 5 No. 1 damping fluids 5 * 4 of dot matrix is to the substrate of selecting on the right of substrate from top to bottom, and the array of 5 No. 2 damping fluids 5 * 4 of dot matrix is to the substrate of putting on the left side of substrate.Temperature is controlled at 25 ℃ of room temperatures, and humidity is controlled at about 70%.
(2) combining of probe and epoxy group(ing): behind the point sample, chip is put into chip cartridges, in 37 ℃ of climatic chambers static 24 hours.
(3) develop a film
A. preparing washing liquid 1 (2xSSC/0.2%SDS), washings 2 (1xSSC);
B. chip is placed the chip staining jar, be immersed in the washings 1, put down, mention chip under the room temperature lightly 15 times; Chip is transferred in the staining plate that fills washings 2, put into, mention chip under the room temperature lightly 15 times;
C. shift out staining jar.Use the deionized water cleaning down, washing time is no less than 2 times; With the dry chip of centrifuging (200 * g for 10s).
(4) scanning
A. a slice sample is put into scanner, through dedicated scan appearance software PM intensity is adjusted to Cy3/PMT600, Power100 clicks start button, beginning coarse scan sample, and it is as shown in Figure 4 to obtain scanning picture;
B. carefully sweep signal best 25 * 4 protein arrays, 25 * 4DNA arrays.Read Cy3 background signal intermediate value, some signal intermediate value and SD value reading, and calculate its discrimination factor CV value and SNR S/N value, as shown in table 1;
C. finish scanning, take out sample.
Present embodiment is put into common amino sheet, aldehyde radical sheet, agar sugar-tablet and ThemoFisher epoxy substrate as a comparison simultaneously in the process of point sample.
Table 1 epoxy substrate scan-data
Dna probe
The albumen probe
Claims (6)
1. the preparation method of an epoxy group modified bio-chip substrate comprises:
(1) high-boron-silicon glass sheet surface treatment: the high-boron-silicon glass sheet that supersound process is crossed is immersed in the mixed solution of the vitriol oil, hydrogen peroxide and ultrapure water that volume ratio is 4:1:20; 80～130 ℃ of held 15～30 minutes; Be soaked in after cooling, the ultrapure water flushing in concentrated hydrochloric acid that volume ratio is 1:1 and the alcohol mixed solution 3～24 hours; Ultrapure water cleans 3～5 times, dries 15～30 minutes for 110～140 ℃;
(2) preparation of epoxy silane coupling solution: preparation contains the absolute ethyl alcohol or the aqueous isopropanol of 1～3vol%GPTMS, 1～3vol% Glacial acetic acid min. 99.5; Magnetic agitation is warming up to 60～80 ℃, refluxes 1～2 hour; Reduce to room temperature, whole process is carried out under the lucifuge condition;
(3) pH=7 of the above-mentioned silane coupler solution of adjusting, magnetic agitation 3～5 minutes; Add the high-boron-silicon glass sheet after surface treatment rapidly; At 25～30 ℃; Relative humidity is to place in 30～50% climatic chambers 2～24 hours, takes out the back and cleans with absolute ethyl alcohol earlier, and then wash with ultrapure water; Dried 15～30 minutes, and promptly got epoxy group modified bio-chip substrate for 110～140 ℃.
2. according to the preparation method of the said a kind of epoxy group modified bio-chip substrate of claim 1, it is characterized in that: the high-boron-silicon glass sheet physical dimension in the said step (1) is that 25.0mm ± 0.2mm * 75.6mm ± 0.2mm, thickness are 1.0mm ± 0.02mm.
3. according to the preparation method of the said a kind of epoxy group modified bio-chip substrate of claim 1, it is characterized in that: ultrasonic in the said step (1) is in ultrapure water ultrasonic 5～10 minutes.
4. according to the preparation method of the said a kind of epoxy group modified bio-chip substrate of claim 1; It is characterized in that: the preparation of epoxy silane coupling solution in the said step (2): get 99.8% absolute ethyl alcohol 77.6ml; GPTMS2.4ml, Glacial acetic acid min. 99.5 2.4ml joins in the there-necked flask, is heated to 60 ℃ with oil bath; Magnetic agitation refluxed 2 hours, reduce to room temperature then.
5. according to the preparation method of the said a kind of epoxy group modified bio-chip substrate of claim 1; It is characterized in that: the preparation of epoxy silane coupling solution in the said step (2): get 1mlGPTMS; The 98ml Virahol, 1ml acetic acid joins in the there-necked flask, is heated to 80 ℃ with oil bath; Magnetic agitation refluxed 1 hour, reduce to room temperature then.
6. according to the preparation method of the said a kind of epoxy group modified bio-chip substrate of claim 1, it is characterized in that: use strong aqua to regulate the pH of silane coupler solution in the said step (3).
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| CN106596961A (en) * | 2016-12-13 | 2017-04-26 | 湖南圣湘生物科技有限公司 | Construction method of micron-structure biochip high in sensitivity |
| CN108503565B (en) * | 2018-04-04 | 2019-08-09 | 大连理工大学 | A kind of bio-chip substrate, preparation method and application |
| CN108318684A (en) * | 2018-04-04 | 2018-07-24 | 南京农业大学 | A kind of the visible protein chip preparation method and detection method of detection pig parvoviral antibody |
| CN109001460B (en) * | 2018-06-13 | 2021-05-11 | 爱必信（上海）生物科技有限公司 | Protein chip biomarker screening method |
| CN111266142A (en) * | 2020-03-19 | 2020-06-12 | 京东方科技集团股份有限公司 | Detection chip, modification method thereof and reaction system |
| CN114479126B (en) * | 2022-03-07 | 2024-04-12 | 成都福实生物科技有限公司 | Method for preparing hydrogel capable of simulating in-vivo ECM stiffness microenvironment and application of hydrogel |
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| CN1552895A (en) * | 2003-06-04 | 2004-12-08 | 哈尔滨基太生物芯片开发有限责任公司 | Substrate surface derivatization treating technology for gene chip |
| EP1726661A1 (en) * | 2005-06-27 | 2006-11-29 | Hitachi Software Engineering Co., Ltd. | Method for manufacturing a biosensor element |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1552895A (en) * | 2003-06-04 | 2004-12-08 | 哈尔滨基太生物芯片开发有限责任公司 | Substrate surface derivatization treating technology for gene chip |
| EP1726661A1 (en) * | 2005-06-27 | 2006-11-29 | Hitachi Software Engineering Co., Ltd. | Method for manufacturing a biosensor element |
Non-Patent Citations (2)
| Title |
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| 李德亮等.二氧化硅表面的GPTMS修饰.《化学进展》.2008,第20卷(第7/8期),1115-1121. * |
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