CN101878785A - Tick-killing biological control agent and preparation method thereof - Google Patents
Tick-killing biological control agent and preparation method thereof Download PDFInfo
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- CN101878785A CN101878785A CN2010102117079A CN201010211707A CN101878785A CN 101878785 A CN101878785 A CN 101878785A CN 2010102117079 A CN2010102117079 A CN 2010102117079A CN 201010211707 A CN201010211707 A CN 201010211707A CN 101878785 A CN101878785 A CN 101878785A
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- tick
- beauveria bassiana
- biological control
- control agent
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to a tick-killing biological control agent. Suspension emulsion or powder can be prepared from 90 to 99 mass percent of spore powder of beauveria bassiana and 1 to 10 mass percent of celangulin which serve as main raw materials. The spore powder of beauveria bassiana is obtained by performing vacuum drying and pulverization on conidia which is obtained by performing liquid-solid two-phase fermentation on the beauveria bassiana, and vibrating the pulverized conidia by using a 120 to 200-mesh sieve, wherein the spore content is counted by using a blood counting chamber. The tick-killing biological control agent can be prepared into various required formulations such as the powder or the suspension emulsion and the like. The tick-killing biological control agent has the advantages of strong pathogenicity to ticks, safety for humans, domestic animals and other living beings, no environmental pollution, difficult generation of medicament resistance, better comprehensive prevention and control effect, high compatibility between the beauveria bassiana and the celangulin in the compounded biological tick-killing agent, better effect of killing the ticks compared with singly used beauveria bassiana or celangulin, lower cost, simpler production process and easy operation.
Description
Technical field
The present invention relates to the technology of insecticidal preparation, especially for a kind of tickicide biological control novel formulation of prevention and control tick and preparation method thereof.
Background technology
Tick (Tick) is under the jurisdiction of Acarina, Ixodoidea, is the common class epizoa of animal body surface, is distributed widely in all over the world, can be by biting affects humans, mammal, birds, reptiles and amphibian animal.The harm of tick directly translates into by the productivity of biting the uneasy and wound infection of animal disturbance and causing and descends, and is a large amount of when parasitic, and the toxin of tick secretion causes that animal benumbs, and causes the tick paralysis.In addition, tick is Lyme disease, spotted fever, Q heat, tick-borne encephalitis, Hemorrhagic fever, this parasitosis of BABEI, theileriosis and does not have transmission of disease media such as slurry disease that its effect of bringing into play in the groove at some disease of natural focus is only second to the mosquito class.Tick and tick pass sick (TTBD) and not only cause serious economic loss to livestock breeding, return the production of human health and animal and bring threat, have become the public health problem that global medical circle and animal doctor educational circles extraordinarily pay close attention to.At present to the controlling mode of tick mainly based on the chemicals control, but concern along with chemical sproof generation of tick and public health problems such as people's environmental pollution and medicament residue, being applied in to a certain extent of this method is restricted, and people transfer to sight on the green prevention and control means of biological control.
Beauveria bassiana (Beauveria bassiana) is a class broad spectrum activity insect pathogenic fungus, can infect 700 various insects of 149 sections of 15 orders, and have pathogenic strong, be easy to cultivate, raw material is cheap and easy to get, to plurality of advantages such as warm blooded animal are harmless, free from environmental pollution, thereby become present domestic and international application insect pathogenic fungus the most widely.Domestic research at beauveria bassiana focuses mostly at forestry pest in using, and the control of tick class be yet there are no report.The foreign scholar has done many relevant experiments with beauveria bassiana control tick, the result shows that its each vegetative stage to multiple tick (ovum, young tick, if tick, become tick and the female tick of full blood) all has lethal effect to a certain degree, and white muscardine fungi is the strongest to the lethal effect of larval stage, can reach 100% lethality rate.Moreover, beauveria bassiana also influences the casting off a skin etc. of hatching, tick of the laying eggs of tick, tick, even can reduce tick offspring's fertility, has broad prospects in the research and development of tickicide novel formulation.Though white muscardine fungi has plurality of advantages, the maximum that influences the performance of its effectiveness hinder be insecticidal rate slowly, aspects such as prevention and control effect instability, therefore seriously limited its extensive use aspect the preventing and treating of tick.
For this reason, the tickicide medicine of fungi tickicide preparation and low dosage is mixed, and both can solve a fungi tickicide difficult problem slowly, can reduce medicine again to the pollution of environment and the problems such as pesticide resistance of alleviation tick, really reaches the purpose to the green prevention and control of tick.At present, there are following problem in chemical tickicide medicine and beauvericin insecticide mixture preparation: (1) chemicals and white muscardine fungi preparation biocompatibility are relatively poor, influence the performance fully of fungi preparation effect; (2) the chemicals environmental pollution is serious, and chemical residual is bigger, and is bigger to people and animals' harm; (3) be that the formulation biological study is not deep enough, lack the suitable formulation of high technology content; (4) though good effect is showed in the biological control of tick in the laboratory, also be not applied in the practice.
Summary of the invention
Tick-killing biological control agent that technical problem to be solved by this invention provides that a kind of compatibility is good, efficient, the white muscardine fungi of low toxicity, low-residual and Celastrus angulatus are composite and preparation method thereof.
A kind of tick-killing biological control agent is a primary raw material with beauveria bassiana spore powder and Celastrus angulatus, presses formulated suspension emulsion of mass ratio or the pulvis of beauveria bassiana spore powder 90-99%, Celastrus angulatus 1-10%.
The mass ratio of described white muscardine fungi and Celastrus angulatus is: beauveria bassiana spore powder 93-96%, Celastrus angulatus 4-7%.
Described beauveria bassiana spore powder is after 120-200 order shaker vibration obtains beauveria bassiana spore powder by liquid-solid two-phase fermentation, vacuum drying, pulverizing.
Suspension emulsion of the present invention is prepared from as follows:
A. weigh content and be the beauveria bassiana spore powder of 20,000,000,000/gram, add Celastrus angulatus in proportion and mix, it is in 1: 2 the vegetable oil that mix preparation is dissolved in mass ratio;
B. the 1-5% that presses the mix preparation total amount adds ultraviolet protective agent, and the 1-3% interpolation emulsifier by total amount mixes.
Described pulvis is prepared from as follows:
A. weighing content is the beauveria bassiana spore powder of 20,000,000,000/gram, adds Celastrus angulatus in proportion and mixes;
B. the 1-5% that presses the mix preparation total amount adds ultraviolet protective agent, adds the kaolin of gross weight 95%, mixes.
Described vegetable oil is: rape seed oil, siritch or salad oil; Described ultraviolet protective agent is: ascorbic acid or fluorescein sodium.Described emulsifier is: aliphatic alcohol polyethenoxy (3) ether (AEO-3) or aliphatic alcohol polyethenoxy (4) ether (AEO-4).
Celastrus angulatus belongs to the plant insecticide of a kind of efficient, wide spectrum, low toxicity, low-residual, in test dose animal is not had teratogenesis, mutagenesis, carcinogenesis, based on stomach poison function.Adopt the Celastrus angulatus and the white muscardine fungi preparation of sublethal dose or inferior sublethal dose to be mixed, shorten the action time of white muscardine fungi preparation on the one hand owing to the adding Celastrus angulatus, make microbial inoculum synergy; On the other hand owing to add white muscardine fungi, Celastrus angulatus just can play the tickicide effect at sublethal dose or inferior sublethal dose, reduced drug dose significantly, can alleviate the problem that insect develops immunity to drugs to chemicals and chemicals pollutes environment to a great extent, in the hope of reaching the purpose of tick green prevention and control.
The conidium that tick-killing biological control agent of the present invention adopts white muscardine fungi to obtain by the liquid-solid two-phase fermentation obtains beauveria bassiana spore powder after vacuum drying, pulverizing, the vibration of 120-200 mesh sieve, count spore content with blood counting chamber.This tick-killing biological control agent can be made into the formulation of various needs, for example pulvis or suspension emulsion etc.
The present invention has following advantage:
1, complex biological tickicide preparation is strong to the tick pathogenicity, and is simultaneously to bio-safeties such as people, animal, free from environmental pollution;
2, complex biological tickicide preparation is difficult for developing immunity to drugs, and integrated control effect is better;
3, the compatibility of white muscardine fungi and Celastrus angulatus is better in the complex biological tickicide preparation, compares with independent use white muscardine fungi or Celastrus angulatus, and the tickicide effect strengthens;
4, production cost is lower, and production technology is simpler, and is easy to operate.
Embodiment
Embodiment 1
White muscardine fungi Celastrus angulatus suspension emulsion
1, weighs content and be 20,000,000,000/9.0 kilograms of the beauveria bassiana spore powders of gram, adds 1.0 kilograms of Celastrus angulatus mixing, add 20 liters of rape seed oils mix preparation is dissolved;
2, add 0.1 kilogram of ascorbic acid, 0.1 kilogram of AEO-3, abundant stirring and evenly mixing gets emulsifier in blender, packing.
Embodiment 2
White muscardine fungi Celastrus angulatus suspension emulsion
1, weighs content and be 20,000,000,000/9.6 kilograms of the beauveria bassiana spore powders of gram, adds 0.4 kilogram of Celastrus angulatus mixing, add 20 liters of salad oils mix preparation is dissolved;
2, add 0.5 kilogram of fluorescein sodium, 0.3 kilogram of AEO-4, abundant stirring and evenly mixing gets emulsifier in blender, packing.
Embodiment 3
White muscardine fungi Celastrus angulatus pulvis
1, weighs content and be 20,000,000,000/9.9 kilograms of the beauveria bassiana spore powders of gram, adds 0.1 kilogram of Celastrus angulatus mixing;
2, add 9.5 kilograms of kaolin, 0.1 kilogram of ascorbic acid, in blender, fully pack behind the stirring and evenly mixing.
Embodiment 4
The preparation of white muscardine fungi Celastrus angulatus pulvis
1, weighs content and be 20,000,000,000/9.3 kilograms of the beauveria bassiana spore powders of gram, adds 0.7 kilogram of Celastrus angulatus mixing;
2, add 9.5 kilograms of kaolin, 0.5 kilogram of fluorescein sodium, in blender, fully pack behind the stirring and evenly mixing.
Claims (7)
1. a tick-killing biological control agent is characterized in that with beauveria bassiana spore powder and Celastrus angulatus be primary raw material, presses formulated suspension emulsion of mass ratio or the pulvis of beauveria bassiana spore powder 90-99%, Celastrus angulatus 1-10%.
2. a kind of tick-killing biological control agent according to claim 1 is characterized in that the mass ratio of described beauveria bassiana spore powder and Celastrus angulatus is: beauveria bassiana spore powder 93-96%, Celastrus angulatus 4-7%.
3. a kind of tick-killing biological control agent according to claim 1 and 2 is characterized in that described beauveria bassiana spore powder is after 120-200 order shaker vibration obtains beauveria bassiana spore powder by liquid-solid two-phase fermentation, vacuum drying, pulverizing.
4. a kind of tick-killing biological control agent according to claim 1 is characterized in that described suspension emulsion is prepared from as follows:
A. weigh content and be the beauveria bassiana spore powder of 20,000,000,000/gram, add Celastrus angulatus in proportion and mix, it is in 1: 2 the vegetable oil that mix preparation is dissolved in mass ratio;
B. the 1-5% that presses the mix preparation total amount adds ultraviolet protective agent, and the 1-3% interpolation emulsifier by total amount mixes.
5. a kind of tick-killing biological control agent according to claim 1 is characterized in that described pulvis is prepared from as follows:
A. weighing content is the beauveria bassiana spore powder of 20,000,000,000/gram, adds Celastrus angulatus by proportioning and mixes;
B. the 1-5% that presses the mix preparation total amount adds ultraviolet protective agent, adds the kaolin of gross weight 95%, mixes.
6. a kind of tick-killing biological control agent according to claim 4 is characterized in that described vegetable oil is: rape seed oil, siritch or salad oil; Described emulsifier is: aliphatic alcohol polyethenoxy (3) ether or aliphatic alcohol polyethenoxy (4) ether;
7. according to claim 4 or 5 described a kind of tick-killing biological control agents, it is characterized in that described ultraviolet protective agent is ascorbic acid or fluorescein sodium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102117079A CN101878785A (en) | 2010-06-26 | 2010-06-26 | Tick-killing biological control agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102117079A CN101878785A (en) | 2010-06-26 | 2010-06-26 | Tick-killing biological control agent and preparation method thereof |
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| CN101878785A true CN101878785A (en) | 2010-11-10 |
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| CN2010102117079A Pending CN101878785A (en) | 2010-06-26 | 2010-06-26 | Tick-killing biological control agent and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067886A (en) * | 2011-01-24 | 2011-05-25 | 吉林省农业科学院 | Beauveryia bassiana pesticide suspoemulsion and preparation method thereof |
| CN102428965A (en) * | 2011-09-22 | 2012-05-02 | 中国水稻研究所 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
| CN103081912A (en) * | 2013-01-25 | 2013-05-08 | 浙江农林大学 | Celastrus angulatus and cnidium lactone compounded pesticide and preparation method thereof |
| CN106305847A (en) * | 2016-08-22 | 2017-01-11 | 温美群 | Sorghum seed-dressing agent |
| CN106942281A (en) * | 2017-05-02 | 2017-07-14 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and Celastrus angulatus |
-
2010
- 2010-06-26 CN CN2010102117079A patent/CN101878785A/en active Pending
Non-Patent Citations (6)
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| 任巧云等: "八种杀蜱药物与球孢白僵菌分生孢子的生物相容性 ", 《中国兽医科学》 * |
| 任巧云等: "八种杀蜱药物与球孢白僵菌分生孢子的生物相容性", 《中国兽医科学》, no. 05, 20 May 2009 (2009-05-20) * |
| 赵锦年，陈爱芬: "被害树干植入具球孢白僵菌的麦麸颗粒饲料防治松墨天牛 ", 《河北林业科技》 * |
| 赵锦年，陈爱芬: "被害树干植入具球孢白僵菌的麦麸颗粒饲料防治松墨天牛", 《河北林业科技》, no. 04, 30 December 1994 (1994-12-30) * |
| 顿玉慧等: "新型球孢白僵菌孢子悬乳剂的高效杀蚜活性及其评价方法 ", 《微生物学报》 * |
| 顿玉慧等: "新型球孢白僵菌孢子悬乳剂的高效杀蚜活性及其评价方法", 《微生物学报》, no. 06, 4 December 2003 (2003-12-04) * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067886A (en) * | 2011-01-24 | 2011-05-25 | 吉林省农业科学院 | Beauveryia bassiana pesticide suspoemulsion and preparation method thereof |
| CN102428965A (en) * | 2011-09-22 | 2012-05-02 | 中国水稻研究所 | Specialized form spore powder of helminthosporium carposaprum echinochloa crusgalli |
| CN103081912A (en) * | 2013-01-25 | 2013-05-08 | 浙江农林大学 | Celastrus angulatus and cnidium lactone compounded pesticide and preparation method thereof |
| CN103081912B (en) * | 2013-01-25 | 2014-12-10 | 浙江农林大学 | Celastrus angulatus and cnidium lactone compounded pesticide and preparation method thereof |
| CN106305847A (en) * | 2016-08-22 | 2017-01-11 | 温美群 | Sorghum seed-dressing agent |
| CN106942281A (en) * | 2017-05-02 | 2017-07-14 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and Celastrus angulatus |
| CN106942281B (en) * | 2017-05-02 | 2018-03-20 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and Celastrus angulatus |
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Application publication date: 20101110 |