CN101869100A - Low-temperature preservation bag - Google Patents
Low-temperature preservation bag Download PDFInfo
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- CN101869100A CN101869100A CN201010185273A CN201010185273A CN101869100A CN 101869100 A CN101869100 A CN 101869100A CN 201010185273 A CN201010185273 A CN 201010185273A CN 201010185273 A CN201010185273 A CN 201010185273A CN 101869100 A CN101869100 A CN 101869100A
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- bag
- edge sealing
- sealing line
- low temperature
- storage bag
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Abstract
The invention relates to a low-temperature preservation bag which comprises a bag body and a pipeline, wherein a liquid outlet pipe and a liquid inlet pipe which are communicated with the inside and the outside of the bag body are arranged at the mouth of the bag body, the liquid inlet pipe is communicated with the pipeline at the outside, the bag body is formed from an upper film and a lower film through edge sealing, the upper film and the lower film are outwards protruded at the part close to an edge sealing line, a certain gap is formed in the bag, and the edge sealing line is positioned at the middle position of the bonded part of the upper film and the lower film. The low-temperature preservation bag of the invention has the advantage of breakage resistance, and meanwhile, the manufacturing method is simple, and the cost is low.
Description
Technical field
The present invention relates to medicine equipment, particularly relate to a kind of profound hypothermia storage bag that active somatic cell is preserved that is used for of new structure, and the method for making this storage bag.
Background technology
Profound hypothermia (196 ℃) is preserved the metabolism that can reduce cell significantly, delay the forfeiture of its function, in view of adopting specific process to be cooled to profound hypothermia biological tissue or organ clinically, and long preservation, when to be needed, again it is pressed the specific process rewarming, so that obtain organism alive.Therefore it is crucial that a kind of suitable anti-profound hypothermia is preserved container, and this container will have certain transparency, so that observe, nontoxic to human body, good physicochemical performance is arranged, must have good resistance to low temperature simultaneously, preserve under-196 ℃ condition, reaching does not have breakage, ne-leakage.Existing flexible PVC bag is subjected to minimum impact and also can damages-40 ℃ of following embrittlement under extremely low temperature, be not suitable for profound hypothermia and preserve.What propose has fluorinated ethylene propylene (FEP) (FEP) profound hypothermia storage bag, polyimides and a tetrafluoroethene composite membrane profound hypothermia storage bag before.The product that such material is made though physicochemical property is good, costs an arm and a leg, and processed complex requires height, the high deficiency of low temperature breakage rate to equipment.There is the low temperature storage bag that adopts the ultra-high-molecular-weight polyethylene multi-layer film structure in Japan, though the low problem that has complex manufacturing technology of breakage rate.American-European most copolymers (EVA) that adopt composite polyolefine, ethylene-vinyl acetate etc. are made the storage bag of anti-the profound hypothermia.
The copolymer of composite polyolefine, ethylene-vinyl acetate (EVA) etc. is made the storage bag of anti-the profound hypothermia; bag mostly adopts single face hot plate and high-frequency induction welding technique; the edge sealing line is horizontal with following film; the edge sealing line is thinner with following film engagement edge place; easily producing stress concentrates; in freezing or course of defrosting, usually breakage can occur, and breakage rate is higher relatively.Patent EP1541342A2 shows that the breakage rate of the EVA profound hypothermia storage bag of Nexell company is 20%, and the breakage rate of the PE profound hypothermia storage bag of Charter company is 10%.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of profound hypothermia storage bag of new version is provided, and has pointed out its preparation method.The problem that profound hypothermia storage bag of the present invention occurs at reality has been carried out structure and improvement manufacture craft, makes this storage bag in profound hypothermia (liquid nitrogen preservations) time Stability Analysis of Structures, and breakage rate is low.
In order to reach the foregoing invention purpose, technical scheme provided by the invention is as follows:
A kind of low temperature storage bag, this storage bag is made up of bag and pipeline, oral area at bag is provided with two drain pipes and feed tube that is communicated with the bag inside and outside, described feed tube is communicated in outside pipeline, described bag is made of last film and following film edge sealing, it is characterized in that the described film of going up is outwards being heaved near edge sealing line position with following film, described edge sealing line is positioned at the centre position of film and following film bonding place.
In the present invention, described edge sealing line is divided into inside portion and outside portion on cross section, and the thickness of described inside portion is greater than the thickness of outside portion.
As a kind of design of optimization, the intersection between described inside portion and outside portion is positioned at 1/4～3/4 place of edge sealing line.
As further optimizing design scheme, described edge sealing line inside portion is big more the closer to the thickness of the inboard edge sealing line of bag, and this inside portion has the gradient of inclination from inside to outside.
Corresponding with above-mentioned inside portion, the thickness of described outside portion is homogeneous thickness, to guarantee the firm degree of edge sealing line.
As the design of an optimization, described drain pipe is positioned at the both sides of feed tube, and the part of drain pipe stretches in the inner chamber of bag, and a part is exposed to the bag outside, is provided with the barrier film that cuts off the inside and outside portion of bag in the pipe of drain pipe.
As a design of further optimizing, the part edge that described drain pipe is positioned at the bag outside is provided with the butterfly wing.Barrier film is arranged in the drain pipe, the heat sealable sealing in upper end, there are two pairs of butterfly wings the centre, and two pairs of interplane cut-out mouths that have a tube wall to approach of butterfly can be twisted into two parts going up the butterfly wing during use easily, pierce through barrier film with needle tubing and stretch in the storage bag, can avoid polluting.
A kind of mould of making above-mentioned low temperature storage bag, this mould is made up of cope match-plate pattern and lower bolster, correspondence is provided with flange on cope match-plate pattern and lower bolster, the shape of this flange is corresponding with the edge sealing line of storage bag, described flange top is an inclined-plane that tilts outside to inside certainly, the top of flange is provided with the groove that holds drain pipe and feed tube on the storage bag, and mould also comprises the tube core rods that 3 and liquid in-out pipe caliber match, and mould is with locating slot and alignment pin outward.
Based on technique scheme, the existing low temperature bag of the low temperature storage bag that the present invention makes has following technological merit:
1. profound hypothermia storage bag of the present invention is positioned at the existence of the part of edge sealing line inboard 1/2 because of the mold flanges inclined-plane, film is in alloying process, the EVA of fusion is to the bag flows inside, make thickness near the edge sealing line of bag inboard greater than thickness near bag outside edge sealing line, and big more the closer to the inboard thickness of bag, on, following film is outwards being heaved near edge sealing line position symmetry, can and make and up and down form certain clearance between the film, can well solve the shortcoming of the storage bag bag adhesion of adopting traditional single face high-frequency welding or heat pressing process, the increase near the inboard edge sealing line of bag thickness can strengthen weld strength in addition.Be positioned at the segment thickness homogeneous in the edge sealing line outside 1/2 in addition, can guarantee weld strength.
2. the edge sealing line welds together a part and two films of the following butterfly wing of drain pipe, and drain pipe is fixed on the bag, can twist into two parts going up the butterfly wing easily during convenient the use, takes out and preserves thing.
Description of drawings
Fig. 1 is the overall structure schematic diagram of low temperature storage bag of the present invention.
Fig. 2 is the cutaway view at low temperature storage bag edge sealing line of the present invention position.
Fig. 3 is the structural representation of drain pipe on the low temperature storage bag of the present invention.
Fig. 4 is the cutaway view at the bag edge sealing line position of three kinds of different designs.
Wherein,
1-bag 2-pipeline 3-edge sealing line 4-feed tube 5-drain pipe 51-butterfly wing 52-cuts off a mouthful 53-barrier film 54-air-vent 55-seal 8-label bag 9-and hangs mouth
Embodiment
Below we come low temperature storage bag of the present invention is described in further detail with specific embodiment in conjunction with the accompanying drawings, but can not limit protection scope of the present invention with this.
Please see Figure 1 earlier, Fig. 1 is the overall structure schematic diagram of low temperature storage bag of the present invention.As seen from the figure, the present invention relates to the low temperature storage bag, this storage bag is made up of bag 1 and pipeline 2.Described bag 1 is formed by the high frequency edge sealing by two films together, the edge sealing line 3 of welding be positioned at bag around.Port position at bag is provided with two drain pipes 5 and a feed tube 4, and described feed tube 4 is communicated in outside pipeline 2.The technological improvement of the property of the present invention is directed to is: with the cross section of storage bag horizontal positioned state: go up film and following film and outwards heaving near edge sealing line position, the edge sealing line of bag is positioned at the centre position of film and following film.With edge sealing line 1/2 medium line is the boundary, is positioned at the segment thickness homogeneous in the edge sealing line outside 1/2, can guarantee weld strength like this, makes bag have certain fastness; Be positioned at of the existence of the part of edge sealing line inboard 1/2 because of the mold flanges inclined-plane, the thickness of the edge sealing line of close bag inboard is greater than the thickness near bag outside edge sealing line, and big more the closer to the inboard thickness of bag, certainly, 1/2 place of selection edge sealing line is a kind of design of optimization as the line of demarcation of inside portion and outside portion, other positions that can also select the edge sealing line are as the line of demarcation, can also adopt in the edge sealing line 1/4～3/4 arbitrarily ratio divide, we call the inside portion to the position near the bag inboard, then be called the outside portion near the outside, then the inside portion is a wedge shape, from outside increase gradually to interior thickness, outside portion thickness homogeneous, its thickness equates with the inside portion thinnest part.The said structure feature can be found out that Fig. 2 is the cutaway view at low temperature storage bag edge sealing line of the present invention position by Fig. 2.As seen from the figure, last film in the low temperature storage bag of the present invention and following film are outwards being heaved near edge sealing line position, this design just can make the distinguishing characteristics that protrudes in bag in the prior art in the inboard of bag formation one, make the bag inboard having bigger distance between film and the following film on the close edge sealing line, form one than large space.Simultaneously, the edge sealing line has part to be projected in the bag, and its effect is the connection of further reinforcing upper and lower film.
By the analysis to prior art, the low temperature storage bag that adopts traditional welding technology is in freezing and course of defrosting, and the storage bag breakage rate is higher.The conclusion of experimental analysis is that problem goes out at edge sealing line that adopts traditional welding technology and following film to be on the same horizontal level, and the edge sealing line is thinner with following film engagement edge place, easily produces stress and concentrates, and it is damaged to cause the low temperature storage bag to occur, and breakage rate is higher relatively.After designing the such edge sealing line structure of the present invention, the edge sealing line is positioned at the centre position of film and following film, for symmetrical structure and inboard thickness are bigger, as shown in Figure 2.This design avoids occurring the stress concentration phenomenon that edge sealing line and film junction became uneven cause, and can also strengthen the weld strength of bag.Listed the side cutaway view of the edge sealing line structure of three kinds of low temperature storage bags among Fig. 6, wherein a type is the edge sealing line side cutaway view of the low temperature storage bag made of traditional handicraft, and the edge sealing line is on the same horizontal level with following film; The b type is the basic model side cutaway view of low temperature storage bag of the present invention, wherein going up film and following film is outwards heaving near edge sealing line position, the edge sealing line of bag is positioned at the centre position of film and following film, formed a big relatively space at the inner close edge sealing line position of bag like this, in addition, the edge sealing line of bag outside is a uniform thickness, the edge sealing operation when being convenient to the bag manufacturing like this; The c type is the improvement of b type, improvements are not uniform thickness of edge sealing line, but the edge sealing line inside portion and outside portion have been divided into, wherein the outside portion is a uniform thickness, then there is certain gradient in the inside portion, the inside portion has just formed the closer to the thick more phenomenon in bag inboard like this, and the gradient that increases part presents bilateral symmetry, can increase the tightness of edge sealing line greatly like this for bag, make the low temperature storage bag in use more firm, greatly reduce the probability that the low temperature storage bag breaks along edge sealing line place.
When design storage bag contour structures and external structure, described drain pipe 5 is arranged at the both sides of feed tube 4, the part of drain pipe 5 and feed tube 4 stretches in the inner chamber of bag, and a part is exposed to the bag outside.The purpose that feed tube 4 is set is perfusion material to be preserved in storage bag, is generally the liquid composition.Drain pipe 5 then is to finish in the preservation task, and heat up thaw after, will preserve material in the bag and extract out.
The part edge that described drain pipe 5 is positioned at the bag outside is provided with the butterfly wing 51, and the butterfly wing is divided into the butterfly wing and the following butterfly wing again.Drain pipe 5 upper ends can adopt seal 52 to carry out the heat seal sealing, and it and the interior barrier film 53 of pipe form airtight space, and the sterilization back has a sterilized space at barrier film 53 to drain pipe upper end heat seal seal place, can avoid pollution during use.The interplane cut-out mouth 52 that has a tube wall to approach of the above-mentioned last butterfly wing and following butterfly can be twisted into two parts going up the butterfly wing during use easily, pierces through barrier film 53 with needle tubing again and stretches in the storage bag.
In order to make above-mentioned low temperature storage bag, we have designed special mould, this mould is dimerous by cope match-plate pattern and lower bolster, correspondingly on a side of side of cope match-plate pattern and lower bolster is provided with flange, and the shape of this flange is corresponding with the edge sealing line of storage bag.Cope match-plate pattern flange on the cope match-plate pattern is the same substantially with lower bolster flange shape on the lower bolster, and is close when being convenient to the two and fitting, and then presses double-layer films.
The top of described cope match-plate pattern flange and lower bolster flange is an inclined-plane that tilts outside to inside certainly, flange is provided with certainly the inclined-plane of inclination outside to inside, above-mentioned inclined-plane operated by rotary motion is in 1/2 position of flange, be that a flange part is the plane for inclined-plane another part, this inclined-plane becomes any one angle in 3 °～8 ° the angular range with the flange plane, just because of oneself inclined-plane setting outside to inside, at film through cope match-plate pattern, during the lower bolster high-frequency welding, under the effect of pressure, make the molten film material of edge sealing line position along the inclined-plane to internal flow, and then can be thicker, in the inner close edge sealing line position curved structure on the cross section of bag near the inboard edge sealing line of bag.
The part of cope match-plate pattern flange is provided with in order to hold the groove of drain pipe and feed tube on the storage bag, also be fixed with at lower bolster comprise 3 the tube core rod to match with feed tube and drain pipe, be provided with alignment pin and lower bolster is provided with locating slot at cope match-plate pattern, be convenient to the corresponding applying of cope match-plate pattern and lower bolster, can also regulate simultaneously the spacing of the two, with the thickness of control edge sealing line.Simultaneously, the locating slot of mould periphery and alignment pin also can guarantee accurate to the uniformity of mould with assurance edge sealing line, guarantee the quality of product.
The more important is in the above-mentioned mould, because EVA has very low vitrification point so can keep certain elasticity at low temperatures, has the good low-temperature resistance performance, and bag and liquid in-out pipe all adopt EVA material and bag that goodish compatibility is arranged.The low temperature storage bag adopts high-frequency induction welding technique twice, the edge sealing line of bag is positioned at the centre of two films, can well guarantee edge sealing line edge film thickness uniformity up and down, last film and following film are outwards being heaved near edge sealing line position, form certain space in bag, can solve the storage bag bag adhesion of adopting traditional single face high-frequency welding or heat pressing process, an edge sealing line edge is thinner, easily stress is concentrated, crackly shortcoming when thawing.
Make low temperature storage bag of the present invention need a power 8KW can twice high frequency output automatic high frequency Bag Making Machine, utilize above-mentioned mould and Bag Making Machine to make the method following steps of low temperature storage bag:
The first step, cope match-plate pattern and lower bolster are individually fixed on the operational module up and down of above-mentioned high-frequency welder, the position of adjusting locating slot position and alignment pin makes its corresponding matching;
High-frequency welder half an hour is opened in second step, energising, sets predetermined welding current and time, makes High-frequency machine be in stable state;
The 3rd step, two films that cut of preparation;
The 4th step, described film is positioned on the lower bolster, and the liquid in-out pipe is through is put on the tube core rod in the middle of two films, place in the groove of bed die;
The 5th step, start power supply, start the heat seal button, after the described cope match-plate pattern of fitting carries out heat seal processing to the lower bolster, stop machine, separate described cope match-plate pattern and lower bolster, after the cooling adhesion-molded storage bag is taken off;
The 6th goes on foot, the storage bag of making is checked, and qualified back to be tested connects exterior line, encapsulates after the sterilization and preserves.
We have made 50 low temperature storage bags with special construction of the present invention, carry out experiment.Found through experiments, 50 low temperature storage bag none breakages in the liquid nitrogen frozen and the experiment of thawing have good frost resistance.
Claims (7)
1. low temperature storage bag, this storage bag is made up of bag and pipeline, oral area at bag is provided with two drain pipes and feed tube that is communicated with the bag inside and outside, described feed tube is communicated in outside pipeline, described bag is made of last film and following film edge sealing, it is characterized in that the described film of going up is outwards being heaved near edge sealing line position with following film, described edge sealing line is positioned at the centre position of film and following film bonding place.
2. a kind of low temperature storage bag according to claim 1 is characterized in that described edge sealing line is divided into inside portion and outside portion on cross section, and the thickness of described inside portion is greater than the thickness of outside portion.
3. a kind of low temperature storage bag according to claim 2 is characterized in that the intersection between described inside portion and outside portion is positioned at 1/4～3/4 place of edge sealing line.
4. a kind of low temperature storage bag according to claim 3 is characterized in that, there are an inclined-plane in described edge sealing line inside portion and horizontal plane, and is big more the closer to the thickness of the inboard edge sealing line of bag.
5. according to claim 3 or 4 described a kind of low temperature storage bags, it is characterized in that the thickness of described outside portion is homogeneous thickness.
6. a kind of low temperature storage bag according to claim 1, it is characterized in that described drain pipe is positioned at the both sides of feed tube, the part of drain pipe stretches in the inner chamber of bag, a part is exposed to the bag outside, is provided with the barrier film that cuts off the inside and outside portion of bag in the pipe of drain pipe.
7. a kind of low temperature storage bag according to claim 6 is characterized in that described drain pipe is provided with two pairs of butterfly wings, and the interplane cut-out mouth that has tube wall to approach of butterfly, the outside port of drain pipe are the heat seal sealing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010185273.XA CN101869100B (en) | 2010-05-26 | 2010-05-26 | Low-temperature preservation bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010185273.XA CN101869100B (en) | 2010-05-26 | 2010-05-26 | Low-temperature preservation bag |
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| Publication Number | Publication Date |
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| CN101869100A true CN101869100A (en) | 2010-10-27 |
| CN101869100B CN101869100B (en) | 2014-01-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201010185273.XA Active CN101869100B (en) | 2010-05-26 | 2010-05-26 | Low-temperature preservation bag |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260538A (en) * | 2016-03-30 | 2017-10-20 | 泰尔茂株式会社 | The manufacture method of fluid bag and fluid bag |
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| EP0253651A2 (en) * | 1986-07-16 | 1988-01-20 | Autologous Blood Corporation | Method for storing and processing blood |
| CN1032318A (en) * | 1987-09-30 | 1989-04-12 | 上海市血液中心 | Medical profound hypothermia storage bag |
| EP0457256A1 (en) * | 1990-05-15 | 1991-11-21 | Nissho Corporation | Blood bag and method for producing the same |
| WO1996014741A2 (en) * | 1994-11-14 | 1996-05-23 | Pall Corporation | Long-term blood component storage system and method |
| CN2389661Y (en) * | 1999-09-29 | 2000-08-02 | 上海市血液中心 | Cord blood separating and conserving multiple-bag |
| CN1287829A (en) * | 2000-10-10 | 2001-03-21 | 上海市血液中心血制品输血器材经营公司 | Blood platelet storage bag and its mfg. method |
| EP1389232B1 (en) * | 2001-05-09 | 2009-09-23 | Bayer Pharmaceuticals Corporation | A cryopreservation bag assembly for mammalian cell lines |
| CN201345874Y (en) * | 2009-01-08 | 2009-11-18 | 孔佑华 | Medical cell tissue cryopreservation bag |
| CN201758709U (en) * | 2010-05-26 | 2011-03-16 | 金卫医疗科技(上海)有限公司 | Low temperature storage bag |
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2010
- 2010-05-26 CN CN201010185273.XA patent/CN101869100B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0253651A2 (en) * | 1986-07-16 | 1988-01-20 | Autologous Blood Corporation | Method for storing and processing blood |
| CN1032318A (en) * | 1987-09-30 | 1989-04-12 | 上海市血液中心 | Medical profound hypothermia storage bag |
| EP0457256A1 (en) * | 1990-05-15 | 1991-11-21 | Nissho Corporation | Blood bag and method for producing the same |
| WO1996014741A2 (en) * | 1994-11-14 | 1996-05-23 | Pall Corporation | Long-term blood component storage system and method |
| CN2389661Y (en) * | 1999-09-29 | 2000-08-02 | 上海市血液中心 | Cord blood separating and conserving multiple-bag |
| CN1287829A (en) * | 2000-10-10 | 2001-03-21 | 上海市血液中心血制品输血器材经营公司 | Blood platelet storage bag and its mfg. method |
| EP1389232B1 (en) * | 2001-05-09 | 2009-09-23 | Bayer Pharmaceuticals Corporation | A cryopreservation bag assembly for mammalian cell lines |
| CN201345874Y (en) * | 2009-01-08 | 2009-11-18 | 孔佑华 | Medical cell tissue cryopreservation bag |
| CN201758709U (en) * | 2010-05-26 | 2011-03-16 | 金卫医疗科技(上海)有限公司 | Low temperature storage bag |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107260538A (en) * | 2016-03-30 | 2017-10-20 | 泰尔茂株式会社 | The manufacture method of fluid bag and fluid bag |
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| CN101869100B (en) | 2014-01-29 |
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