CN101857644B - Alcohol alkali extraction method of aspartic polysaccharide and production process - Google Patents
Alcohol alkali extraction method of aspartic polysaccharide and production process Download PDFInfo
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- CN101857644B CN101857644B CN201010180715A CN201010180715A CN101857644B CN 101857644 B CN101857644 B CN 101857644B CN 201010180715 A CN201010180715 A CN 201010180715A CN 201010180715 A CN201010180715 A CN 201010180715A CN 101857644 B CN101857644 B CN 101857644B
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Abstract
The invention discloses an alcohol alkali extraction method of aspartic polysaccharide and a production process. The extraction method comprises the following steps: taking radix asparagi decoction pieces; adding extraction solvent into the radix asparagi decoction pieces according to the mass volume ratio of 1:15-1:6, wherein, the alcohol concentration of the extraction solvent is 5-50%; adjusting the pH value to be 5-13; extracting for 0.5-2.5 hours in water bath at the temperature of 60-100 DEG C after soaking for 0.5-2.5 hours and filtering; and processing the residues at least once according to the above steps; and combining all the filtrates to obtain the extracting solution. The production process by adopting the extraction method also comprises the following steps: concentrating the extracting solution, adding 95% of alcohol for precipitating; discarding supernatant; adding water with the same amount as the concentrated solution, heating, dissolving and precipitating, centrifuging and collecting the supernatant; then adding 95% of alcohol with the volume 5 times than the concentrated solution for precipitating, centrifuging, and discarding the supernatant; adding a proper amount of 95% of alcohol and stirring, centrifuging and discarding the supernatant; adding proper amount of absolute ethyl alcohol and stirring; collecting the deposit after centrifuging, and freeze drying to obtain the finished products. The method of the invention obviously improves extraction efficiency of aspartic polysaccharide and total sugar content.
Description
Technical field
The present invention relates to a kind of pure alkali extraction method and production technique of Radix Asparagi polysaccharide.
Background technology
Radix Asparagi polysaccharide (Asparagus Cochinchinensis polysaccharide) can significantly improve the protection ratio and the disease resistance of vaccine as the vaccine immunopotentiator of chicken, has a extensive future.Yet there are the low and low problem of total sugar content of extraction yield in classical water extraction and the potass extraction method of extracting the Radix Asparagi polysaccharide at present, so that the production efficiency of Radix Asparagi polysaccharide is low, under-supply, are difficult to meet the need of market.
Summary of the invention
The technical problem that the present invention will solve provides the pure alkali extraction method and the production technique of the high and Radix Asparagi polysaccharide that total sugar content is high of a kind of extraction yield.
For the pure alkali extraction method that solves the problems of the technologies described above Radix Asparagi polysaccharide of the present invention adopts following technical scheme: get the Radix Asparagi medicine materical crude slice; Adding alcohol concn by mass volume ratio 1: 15～1: 6 is 5～50% extraction solvent; Regulate pH to 5～13, soak after 0.5～2.5 hour and extracted 0.5～2.5 hour for 60～100 ℃, filter in water-bath; The same processing of residue at least once, merge each time filtrate extracting solution.
Adopt the production technique of the Radix Asparagi polysaccharide of above-mentioned pure alkali extraction method, comprise the steps: that also concentrated extracting solution gets liquid concentrator to containing crude drug 0.5g/ml, add 5 times of 95% ethanol of volume and carry out alcohol precipitation, discard supernatant to liquid concentrator; Add with the water heating for dissolving deposition that concentrates liquid measure, centrifugal collection supernatant; Add 5 times of 95% ethanol of volume again and carry out alcohol precipitation, the centrifugal supernatant that discards to liquid concentrator; Add an amount of 95% ethanol and stir, the centrifugal supernatant of abandoning again; Add an amount of absolute ethyl alcohol and stir, centrifugal back collecting precipitation, lyophilize promptly gets the Radix Asparagi polysaccharide.
The pure alkali extraction method of Radix Asparagi polysaccharide of the present invention and the extraction yield of production technique and total sugar content all are higher than present classical water extraction and potass extraction method, are applied to produce the Radix Asparagi polysaccharide and can raise the efficiency, meet the need of market.
Embodiment
Below be the embodiment of the pure alkali extraction method of Radix Asparagi polysaccharide of the present invention:
Embodiment 1
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying to constant weight, the adding alcohol concn is 10% extraction solvent 1500ml, regulates pH to 9; Soak after 1.5 hours and extracted 2.5 hours for 80 ℃ in water-bath; Four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
Embodiment 2
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying to constant weight, the adding alcohol concn is 5% extraction solvent 600ml, regulates pH to 13; Soak after 2.5 hours and extracted 1.5 hours for 60 ℃ in water-bath; Four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
Embodiment 3
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying to constant weight, the adding alcohol concn is 30% extraction solvent 1000ml, regulates pH to 5; Soak after 0.5 hour and extracted 0.5 hour for 100 ℃ in water-bath; Four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
Embodiment 4
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying to constant weight, the adding alcohol concn is 50% extraction solvent 800ml, regulates pH to 7; Soak after 2.0 hours and extracted 1.0 hours for 90 ℃ in water-bath; Four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
Below be the classical water extraction of Radix Asparagi polysaccharide and the reference examples of potass extraction method:
Contrast the classical water extraction of 1 Radix Asparagi polysaccharide
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying, add to decoct behind the zero(ppm) water 1500ml and extracted 2.5 hours to constant weight, four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
Contrast the potass extraction method of 2 Radix Asparagi polysaccharide
Get the Chinese medicine Radix Asparagi medicine materical crude slice 100g of drying, add zero(ppm) water 1500ml, regulate pH to 9, soaks after 1.5 hours in 80 ℃ of extractions of water-bath 2.5 hours to constant weight, four layers of filtered through gauze, the same processing of residue once, merge filtrate for twice extracting solution.
The production technique of embodiment 5 Radix Asparagi polysaccharide
The extracting solution that concentrates the foregoing description 1 to 4 and contrast 1 and 2 respectively gets liquid concentrator 200ml to containing crude drug 0.5g/ml, adds 5 times of 95% ethanol to liquid concentrator of volume and carries out alcohol precipitation, slowly adds, and the limit edged stirs, and the room temperature hold over night discards supernatant subsequently; Add 200mL zero(ppm) water heating for dissolving deposition, the centrifugal 10min of 3000r/min collects supernatant; Add 5 times of 95% ethanol to liquid concentrator of volume again and carry out alcohol precipitation, room temperature is placed and is spent the night, and the centrifugal 10min of 3000r/min abandons supernatant; Add an amount of 95% ethanol and stir, centrifugal the same, abandon supernatant again; Add an amount of absolute ethyl alcohol and stir, centrifugal again, last collecting precipitation promptly gets the Radix Asparagi polysaccharide after the lyophilize.
The mensuration of Radix Asparagi polysaccharide and result
Adopt the phenolsulfuric acid method to measure the foregoing description gained Radix Asparagi polysaccharide content, wherein, the Crude polysaccharides extraction yield calculates by following formula:
Crude polysaccharides extraction yield=Crude polysaccharides quality/Radix Asparagi prepared slice quality * 100%.
Mensuration the results list is following:
| Embodiment or reference examples | 1 | 2 | 3 | 4 | Contrast 1 | Contrast 2 |
| Crude polysaccharides extraction yield (%) | 18.64 | 13.95 | 15.74 | 14.98 | 2.99 | 9.01 |
| Total sugar content (%) | 89.80 | 89.34 | 91.55 | 92.60 | 41.13 | 81.95 |
It is thus clear that, adopt pure alkaline process to extract and produce the Radix Asparagi polysaccharide, improved Crude polysaccharides extraction yield and total sugar content significantly, for large-scale production Radix Asparagi polysaccharide from now on provides good operational path, can effectively guarantee the market supply of the vaccine immunopotentiator of chicken.
Claims (2)
1. the pure alkali extraction method of a Radix Asparagi polysaccharide; It is characterized in that this extraction method comprises the steps: to get the Radix Asparagi medicine materical crude slice, adding alcohol concn by mass volume ratio 1: 15～1: 6 is 5～50% extraction solvent, regulates pH to 5～13; Soak after 0.5～2.5 hour and extracted 0.5～2.5 hour for 60～100 ℃ in water-bath; Filter, the same processing of residue at least once, merge each time filtrate extracting solution.
2. according to the production technique of the pure alkali extraction method of the said Radix Asparagi polysaccharide of claim 1; It is characterized in that this technology also comprises the steps: to concentrate said extracting solution and gets liquid concentrator to containing crude drug 0.5g/ml; Add 5 times of 95% ethanol of volume and carry out alcohol precipitation, discard supernatant to liquid concentrator; Add with the water heating for dissolving deposition that concentrates liquid measure, centrifugal collection supernatant; Add 5 times of 95% ethanol of volume again and carry out alcohol precipitation, the centrifugal supernatant that discards to liquid concentrator; Add an amount of 95% ethanol and stir, the centrifugal supernatant of abandoning again; Add an amount of absolute ethyl alcohol and stir, centrifugal back collecting precipitation, lyophilize promptly gets the Radix Asparagi polysaccharide.
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| CN201010180715A CN101857644B (en) | 2010-05-24 | 2010-05-24 | Alcohol alkali extraction method of aspartic polysaccharide and production process |
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| CN201010180715A CN101857644B (en) | 2010-05-24 | 2010-05-24 | Alcohol alkali extraction method of aspartic polysaccharide and production process |
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| CN101857644B true CN101857644B (en) | 2012-10-03 |
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| CN103143012B (en) * | 2013-03-26 | 2015-06-10 | 广西大学 | Traditional Chinese medicine composition and applications in preparation of porcine pseudorabies vaccine immunopotentiator |
| CN110140812A (en) * | 2019-06-03 | 2019-08-20 | 广西大学 | Prawn Chinese herbal medicine immunopotentiator and preparation method thereof |
| CN114343079A (en) * | 2021-12-20 | 2022-04-15 | 广西大学 | Nitrite nitrogen stress resistant Chinese herbal medicine feed additive for penaeus vannamei boone and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557842A (en) * | 2004-02-02 | 2004-12-29 | 中南大学湘雅二医院 | Process for extracting polygonatum polysaccharides, preparing process for medical preparation and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1557842A (en) * | 2004-02-02 | 2004-12-29 | 中南大学湘雅二医院 | Process for extracting polygonatum polysaccharides, preparing process for medical preparation and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| 杜旭华等.抗癌植物药的开发研究――Ⅳ.中药天冬的多糖类抗癌活性成分的提取与分离.《沈阳药科大学学报》.1990,(第03期), * |
| 田洛等.醇碱提取法提取黄芪多糖.《吉林大学学报(理学版)》.2006,(第04期), * |
| 范荣军等.五味子多糖提取工艺的比较研究.《中成药》.2008,(第06期), * |
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