CN101837079B

CN101837079B - Anti-inflammatory analgesic or anti-inflammatory immune medicinal composition, preparation method and application thereof

Info

Publication number: CN101837079B
Application number: CN201010185787.5A
Authority: CN
Inventors: 张樱山; 张国霞; 卢伍党
Original assignee: Gansu Qizheng Tibetan Medicine Co Ltd
Current assignee: Gansu traditional Chinese Medicine Modern Pharmaceutical Engineering Research Institute Co Ltd
Priority date: 2010-05-28
Filing date: 2010-05-28
Publication date: 2014-03-26
Anticipated expiration: 2030-05-28
Also published as: CN101837079A

Abstract

The invention discloses an anti-inflammatory analgesic or anti-inflammatory immune medicinal composition, a preparation method and application thereof. The medicinal composition comprises the following raw material medicament components in part by weight: 100 to 1,000 parts of lamiophlomis rotata, 100 to 1,500 parts of aconitum sinomontanum, 10 to 1,500 parts of myrobalam, 10 to 100 parts of elecampane, 10 to 100 parts of acorus calamus and the like. The composition of the invention has remarkable anti-inflammatory analgesic and anti-inflammatory immune effects, and has a broad prospect.

Description

A kind of anti-inflammatory and antalgic or anti inflammatory immunity pharmaceutical composition and its production and use

Technical field

The present invention relates to a kind of pharmaceutical composition and its production and use, particularly pharmaceutical composition of a kind of anti-inflammatory and antalgic or anti inflammatory immunity and its production and use.

Background technology

Radix Lamiophlomidis Rotatae is the dry herb of dicotyledon Labiatae Radix Lamiophlomidis Rotatae Lamiophlomis rotata (Benth.) Kudo..Its property is sweet, bitter, flat.Often be born in high mountain rubble beach, flood land or the stone matter grassy marshland of height above sea level 3900-5100m.The highlandss such as distribution Tibet, Qinghai, Sichuan, Gansu.There is promoting blood circulation and hemostasis, wind-expelling pain-stopping effect, for traumatic injury, traumatic hemorrhage, rheumatic arthralgia, grasserie, fracture, lumbar strain.

Aconitum sinomontanum Nakai is the dry root of ranunculaceae plant Aconitum sinomontanum Nakai Aconitum Sinomontanum Nakai, and excavate autumn, removes fibrous root and aerial parts, washes away earth, dries.Be distributed in that each county to the east of Yuxian, Hebei Xiaowutai Shan Mountain, Shanxi, Qinghai Sun-Moon Mountain, Shaanxi, Gansu, Hubei send, the province such as Sichuan and Guizhou.Its root is poisonous, is used as medicine, can reducing swelling and alleviating pain, promoting blood circulation to remove blood stasis, dispel the wind, cure mainly fracture, rheumatic lumbago and scelalgia, furuncle, syphilis, cardiopalmus, stomachache, traumatic injury.

Fructus Chebulae is the dry mature fruit of Combretum Racemosum plant Fructus Chebulae Terminalia chebula Retz. or Terminalia chebula Retz. var. tomentella Kurt. Terminaliachebula Retz.var.tomentella Kurt., its fruit medicine.Two seasons of autumn and winter take, and dry.Be distributed in the ground such as Guangdong, Yunnan, Guangxi, Tibet.Astringing intestine to stop diarrhea is astringed the lung, pathogenic fire reducing sore-throat relieving.For chronic diarrhea chronic dysentery, the proctoptosis of having blood in stool, chronic cough is more than, pharyngalgia hoarseness.

The Radix Aucklandiae is the dry root of feverfew Radix Aucklandiae Aucklandia lappa Decne.Autumn, two seasons of winter excavate, and remove silt and fibrous root, cutting, and large vertical profile again becomes lobe, after being dried, hits rough bark.Cultivate the high Mountain area in height above sea level 2500-4000m, in nice and cool Plain and knob, also can grow.Be grown in alpine meadow and shrubbery, be wild plant more, not yet by artificial introducing and planting.Being distributed in the ground such as China Shaanxi, Gansu, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Yunnan, Tibet has introducing and planting, and more with northwestern Yunnan Province plantation, output is larger.There are promoting the circulation of QI to relieve pain, strengthening the spleen to promote digestion effect.For breast gastral cavity distending pain, heavy after dysentery, food stagnation does not disappear, anorexia.The real intestinal antidiarrheal of Radix Aucklandiae (roasted), for the stomachache of having loose bowels.

Rhizoma Acori Calami is Tibetan's conventional crude drugs, is the dry rhizome of aroid Rhizoma Acori Calami Acorus calamus L..Two seasons of autumn and winter excavate, and remove fibrous root and silt, dry.With dry rhizome, be used as medicine.Be born in the Marsh Wetland below height above sea level 2600m.All there is distribution each provinces and regions, the whole nation, and subtropical zone, the temperate zone in the whole Northern Hemisphere all have.Stomach warming, anti-inflammatory analgetic.Be used for mending stomach sun, dyspepsia, food is stagnant, diphtheria, anthrax.

Rhizoma Corydalis is the dry tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang, with tuber, is used as medicine.It is pungent, bitter, temperature.Return liver, spleen channel.When early summer stem and leaf is withered, excavate, remove fibrous root, clean, put in boiling water, to boil to proper and during without the white heart, take out, dry.Be distributed in Jiangsu, Zhejiang, main product Zhejiang.Have and invigorate blood circulation, promoting the circulation of QI, the effect of pain relieving, for the breast side of body, epigastric pain, amenorrhea dysmenorrhea, postpartum stagnation, tumbling and swelling.

Summary of the invention

The object of the invention is to provide a kind of pharmaceutical composition for anti-inflammatory and antalgic and anti inflammatory immunity; Another object of the present invention is to provide the preparation method of this pharmaceutical composition; The object of the invention is also to provide the new pharmaceutical use of this pharmaceutical composition.

The present invention seeks to be achieved through the following technical solutions:

Option A:

The crude drug of pharmaceutical composition of the present invention consists of: Radix Lamiophlomidis Rotatae 100-1000 weight portion, Aconitum sinomontanum Nakai 100-1500 weight portion, Fructus Chebulae's (enucleation) 10-500 weight portion, Radix Aucklandiae 10-100 weight portion, Rhizoma Acori Calami 10-100 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 200-600 weight portion, Aconitum sinomontanum Nakai 300-1000 weight portion, Fructus Chebulae's (enucleation) 30-100 weight portion, Radix Aucklandiae 20-40 weight portion, Rhizoma Acori Calami 20-40 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 610-900 weight portion, Aconitum sinomontanum Nakai 1001-1400 weight portion, Fructus Chebulae's (enucleation) 120-400 weight portion, Radix Aucklandiae 41-90 weight portion, Rhizoma Acori Calami 41-90 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 100-200 weight portion, Aconitum sinomontanum Nakai 100-300 weight portion, Fructus Chebulae's (enucleation) 10-30 weight portion, Radix Aucklandiae 10-20 weight portion, Rhizoma Acori Calami 10-20 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 900 weight portions, Aconitum sinomontanum Nakai 1300 weight portions, Fructus Chebulae's (enucleation) 200 weight portions, the Radix Aucklandiae 80 weight portions, Rhizoma Acori Calami 80 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 334 weight portions, Aconitum sinomontanum Nakai 400 weight portions, Fructus Chebulae's (enucleation) 67 weight portions, the Radix Aucklandiae 33 weight portions, Rhizoma Acori Calami 33 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 110 weight portions, Aconitum sinomontanum Nakai 1450 weight portions, Fructus Chebulae's (enucleation) 20 weight portions, the Radix Aucklandiae 95 weight portions, Rhizoma Acori Calami 15 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 950 weight portions, Aconitum sinomontanum Nakai 110 weight portions, Fructus Chebulae's (enucleation) 15 weight portions, the Radix Aucklandiae 15 weight portions, Rhizoma Acori Calami 95 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 250 weight portions, Aconitum sinomontanum Nakai 950 weight portions, Fructus Chebulae's (enucleation) 35 weight portions, the Radix Aucklandiae 35 weight portions, Rhizoma Acori Calami 25 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 550 weight portions, Aconitum sinomontanum Nakai 350 weight portions, Fructus Chebulae's (enucleation) 95 weight portions, the Radix Aucklandiae 25 weight portions, Rhizoma Acori Calami 35 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 400 weight portions, Aconitum sinomontanum Nakai 650 weight portions, Fructus Chebulae's (enucleation) 450 weight portions, the Radix Aucklandiae 20 weight portions, Rhizoma Acori Calami 20 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 550 weight portions, Aconitum sinomontanum Nakai 800 weight portions, Fructus Chebulae's (enucleation) 255 weight portions, the Radix Aucklandiae 55 weight portions, Rhizoma Acori Calami 55 weight portions.

In option A, pharmaceutical composition crude drug of the present invention adds conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Any one in option A in the preferred following methods of the preparation method of pharmaceutical composition of the present invention:

Method 1: Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20%-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

50%-100% soak with ethanol 1-5 hour by Aconitum sinomontanum Nakai medical material by 1-5 times of weight, continues to use 50%-100% ethanol percolation, collects percolate, concentrated, hydro-oxidation sodium is adjusted PH to 8-13, standing, centrifugal, precipitation is washed to neutrality, is dried to obtain Aconitum sinomontanum Nakai extract; Or by the 50%-100% soak with ethanol 1-5 hour of Aconitum sinomontanum Nakai medical material use 1-5 times of weight, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 7-14, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract;

Fructus Chebulae's (enucleation) medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Or by the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time for Fructus Chebulae's (enucleation) medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, be dried and obtain Fructus Chebulae extract;

Radix Aucklandiae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; Or by the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time for Radix Aucklandiae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, be dried and obtain Radix Aucklandiae extract;

Rhizoma Acori Calami medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Acori Calami extract that to obtain; Or by the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time for Rhizoma Acori Calami medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, be dried and obtain Rhizoma Acori Calami extract;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 1 is more preferably: Radix Lamiophlomidis Rotatae medical material boiled 3 times with the decocting of 12,10,8 times of weight respectively, decocts respectively 2,1.5,1 hours at every turn, collect, merge extractive liquid,, and concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 80% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 10, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Or by 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-300 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract;

Fructus Chebulae's (enucleation) medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Or by 70% ethanol extraction of 12 times of weight 3 times for Fructus Chebulae's (enucleation) medical material, extract 2.5 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Fructus Chebulae extract that to obtain; at every turn

Radix Aucklandiae medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; Or by 70% ethanol extraction of 12 times of weight 3 times for Radix Aucklandiae medical material, extract 2.5 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Radix Aucklandiae extract that to obtain; at every turn

Rhizoma Acori Calami medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Acori Calami extract that to obtain; Or by 70% ethanol extraction of 12 times of weight 3 times for Rhizoma Acori Calami medical material, extract 2.5 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Acori Calami extract that to obtain; at every turn

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 2: Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-3 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material are mixed in proportion, with the decocting of 5-20 times of weight, boil 1-3 time, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material are mixed in proportion, the 20%-dehydrated alcohol extraction of use 5-20 times of weight 1-3 time extracts 0.5-10 hour at every turn, and collection, merge extractive liquid, are concentrated, the dry mixed extract that to obtain;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 2 is more preferably: Radix Lamiophlomidis Rotatae medical material boiled 3 times with the decocting of 12,10,8 times of weight respectively, decocts respectively 2,1.5,1 hours at every turn, collect, merge extractive liquid,, and concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 12 50% ethanol extraction 3 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are mixed in proportion, with the decocting of 12 times of weight, boil 3 times, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are mixed in proportion, with 70% ethanol extraction of 12 times of weight 3 times, extract 2.5 hours at every turn, collect, merge extractive liquid,, concentrated, the dry mixed extract that to obtain;

Method 3: Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-3 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

50%-100% soak with ethanol 1-5 hour by Aconitum sinomontanum Nakai medical material by 1-5 times of weight, continues to use 50%-100% ethanol percolation, collects percolate, concentrated, hydro-oxidation sodium is adjusted PH to 8-13, standing, centrifugal, precipitation is washed to neutrality, is dried to obtain Aconitum sinomontanum Nakai extract; Or by the 50%-100% soak with ethanol 1-5 hour of Aconitum sinomontanum Nakai medical material use 1-5 times of weight, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, use dissolving with hydrochloric acid residue, centrifugal, get supernatant, add ammonia spirit, supernatant is passed through to cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, with ethanolysis absorption, collect alcoholic solution again, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 7-14, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, cleaning, drying, be ground into fine powder, be mixed in proportion to obtain mixing fine powders;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixing fine powders are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 3 is more preferably: Radix Lamiophlomidis Rotatae medical material boiled 3 times with the decocting of 12,10,8 times of weight respectively, decocts respectively 2,1.5,1 hours at every turn, collect, merge extractive liquid,, and concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 12 50% ethanol extraction 3 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 1-180 μ m, be mixed in proportion to obtain mixed powder;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 10-100 μ m, be mixed in proportion to obtain mixed powder;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 4: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: gets Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, adds 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, and each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material and be ground into respectively the medicated powder that particle diameter is 40-180 μ m, be mixed to get mixed powder; By above dry cream and mixed powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: gets Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, adds 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, and each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material and be ground into respectively the superfine powder that particle diameter is 1-40 μ m, be mixed to get mixing superfine powder; By above dry cream and mixing superfine powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, 60% alcohol reflux 2 times that adds 8 times of weight after mixing, each 1.5 hours, merge extractive liquid,, filter, filtrate is concentrated, dry getting dry extract, separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, 60% alcohol reflux 2 times that adds 8 times of weight after mixing, each 1.5 hours, merge extractive liquid,, filter, filtrate is concentrated, dry getting dry extract, separately get Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make tablet.

Method 5: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, clean, dry, be ground into fine powder, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 5 is more preferably: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, clean, dry, be ground into the medicated powder that particle diameter is 40-180 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 5 is more preferably: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are carried out respectively to remove impurity, clean, dry, be ground into the superfine powder that particle diameter is 1-40 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 6: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material, 40%-100% alcohol reflux 1-3 time that adds 1-10 times of weight after mixing, each 0.5-5 hour, collect, merge extractive liquid, filter, reclaim ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 6 is more preferably: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material, 70% alcohol reflux 2 times that adds 5 times of weight after mixing, each 2 hours, collect, merge extractive liquid, filter, reclaim ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Option b:

The crude drug of pharmaceutical composition of the present invention consists of: Radix Lamiophlomidis Rotatae 100-1000 weight portion, Aconitum sinomontanum Nakai 100-1500 weight portion, Fructus Chebulae's (enucleation) 10-500 weight portion, Radix Aucklandiae 10-100 weight portion, Rhizoma Acori Calami 10-100 weight portion, Rhizoma Corydalis 30-100 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 200-600 weight portion, Aconitum sinomontanum Nakai 300-1000 weight portion, Fructus Chebulae's (enucleation) 30-100 weight portion, Radix Aucklandiae 20-40 weight portion, Rhizoma Acori Calami 20-40 weight portion, Rhizoma Corydalis 50-80 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 610-900 weight portion, Aconitum sinomontanum Nakai 1001-1400 weight portion, Fructus Chebulae's (enucleation) 120-400 weight portion, Radix Aucklandiae 41-90 weight portion, Rhizoma Acori Calami 41-90 weight portion, Rhizoma Corydalis 81-99 weight portion.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 900 weight portions, Aconitum sinomontanum Nakai 1300 weight portions, Fructus Chebulae's (enucleation) 200 weight portions, the Radix Aucklandiae 80 weight portions, Rhizoma Acori Calami 80 weight portions, Rhizoma Corydalis 90 weight portions.

The crude drug composition of pharmaceutical composition of the present invention is preferably: Radix Lamiophlomidis Rotatae 334 weight portions, Aconitum sinomontanum Nakai 400 weight portions, Fructus Chebulae's (enucleation) 67 weight portions, the Radix Aucklandiae 33 weight portions, Rhizoma Acori Calami 33 weight portions, Rhizoma Corydalis 42 weight portions.

In option b, pharmaceutical composition crude drug of the present invention adds conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Any one in option b in the preferred following methods of the preparation method of pharmaceutical composition of the present invention:

Method 1: Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-3 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the decocting of 5-20 times of weight, boil 1-3 time, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, the 20%-dehydrated alcohol extraction of use 5-20 times of weight 1-3 time extracts 0.5-10 hour at every turn, and collection, merge extractive liquid, are concentrated, the dry mixed extract that to obtain;

Method 1 is more preferably: Radix Lamiophlomidis Rotatae medical material boiled 3 times with the decocting of 12,10,8 times of weight respectively, decocts respectively 2,1.5,1 hours at every turn, collect, merge extractive liquid,, and concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 12 50% ethanol extraction 3 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the decocting of 12 times of weight, boil 3 times, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with 70% ethanol extraction of 12 times of weight 3 times, extract 2.5 hours at every turn, collect, merge extractive liquid,, concentrated, the dry mixed extract that to obtain;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into fine powder, be mixed in proportion to obtain mixing fine powders;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 1-180 μ m, be mixed in proportion to obtain mixed powder;

Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 10-100 μ m, be mixed in proportion to obtain mixed powder;

Method 3: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 3 is more preferably: gets Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, adds 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, and each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material and be ground into respectively the medicated powder that particle diameter is 40-180 μ m, be mixed to get mixed powder; By above dry cream and mixed powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 3 is more preferably: gets Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, adds 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, and each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material and be ground into respectively the superfine powder that particle diameter is 1-40 μ m, be mixed to get mixing superfine powder; By above dry cream and mixing superfine powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 3 is more preferably: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, 60% alcohol reflux 2 times that adds 8 times of weight after mixing, each 1.5 hours, merge extractive liquid,, filter, filtrate, concentrated, dry, gets dry extract, separately get Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable tablet;

Method 4: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into fine powder, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into the medicated powder that particle diameter is 40-180 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation;

Method 4 is more preferably: Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into the superfine powder that particle diameter is 1-40 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 5: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material, 40%-100% alcohol reflux 1-3 time that adds 1-10 times of weight after mixing, each 0.5-5 hour, collect, merge extractive liquid, filter, reclaim ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 5 is more preferably: get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material, 70% alcohol reflux 2 times that adds 5 times of weight after mixing, each 2 hours, collect, merge extractive liquid, filter, reclaim ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 6: Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20%-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Corydalis tuber medicinal material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Corydalis extract that to obtain; Or by the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time for corydalis tuber medicinal material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, be dried and obtain Rhizoma Corydalis extract;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract, Rhizoma Acori Calami extract and Rhizoma Corydalis extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form, include but not limited to tablet, capsule, powder, soft capsule, drop pill, honeyed pill, pill, granule, soft extract with bee honey agent, slow releasing preparation, quick releasing formulation, controlled release preparation, oral liquid, ejection preparation or external preparation.

Method 6 is more preferably: Radix Lamiophlomidis Rotatae medical material boiled 3 times with the decocting of 12,10,8 times of weight respectively, decocts respectively 2,1.5,1 hours at every turn, collect, merge extractive liquid,, and concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Corydalis tuber medicinal material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Corydalis extract that to obtain; Or by 70% ethanol extraction of 12 times of weight 3 times for corydalis tuber medicinal material, extract 2.5 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Corydalis extract that to obtain; at every turn

Pharmaceutical composition of the present invention has following beneficial effect:

(1) the invention provides a kind of new pharmaceutical composition with anti-inflammatory and antalgic and anti inflammatory immunity, met clinical needs.

(2) each proportioning of the present composition has been carried out acetic acid and is caused mouse writhing experiment, thermostimulation and cause the experiment of mice pain whipping, mice caused by dimethylbenzene xylene ear swelling test, carrageenin and cause rat paw edema experiment, the impact of adjuvant type rats with arthritis immune system (the related immune factor in organ index and serum) is tested, thereby has drawn the optimum formula of pharmaceutical composition of the present invention.

(3) present composition has significant anti-inflammatory and antalgic and antiinflammatory immunity function, is with a wide range of applications.

(4) the various dosage forms of the present composition can be by those skilled in the art, according to the conventional production method preparation of pharmaceutical field.

Experimental example and embodiment are used for further illustrating but are not limited to the present invention below.

1 three kinds of pharmaceutical composition Dichlorodiphenyl Acetates of experimental example cause the impact of mouse writhing

1. medicine and reagent: diclofenac potassium (positive control drug), Novartis Pharma AG, lot number: X0035; Glacial acetic acid, Rui Jin, Tianjin special chemicals company limited, analytical pure, lot number: 20070522;

Pharmaceutical composition first: (preparation method: get 30% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material 334g, merge extractive liquid,, reclaims ethanol to without alcohol taste, continue to add water to extracting liquid volume 5 times, centrifugal, get supernatant concentration to dry, obtain Radix Lamiophlomidis Rotatae extract; Separately get 60% soak with ethanol of 3 times of weight for Aconitum sinomontanum Nakai medical material 400g, emit soak, and continuation ethanol percolation, collect alcoholic solution, and reclaim ethanol, use dissolving with hydrochloric acid residue, centrifugal, get supernatant, add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; The extract of above Radix Lamiophlomidis Rotatae and Aconitum sinomontanum Nakai is mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable tablet.)；

Pharmaceutical composition second of the present invention: (in description, embodiment 10 is prepared from according to the present invention);

Pharmaceutical composition third of the present invention: (in description, embodiment 25 is prepared from according to the present invention);

When evaluating, three kinds of pharmaceutical composition pharmacologically actives all establish high and low dose group, electronic balance, Haikang, Shanghai Electronic Instruments Plant; Stopwatch, mouse stomach device, syringe.

2. animal: Kunming mouse, male and female half and half, body weight 20 ± 2g, is provided the quality certification number by Lanzhou Institute of Biological Products's animal center: the moving word 08-005 of doctor.

3. experimental technique: choose 80 of healthy Kunming mouses, by body weight, be divided at random 8 groups, every group 10, male and female half and half, be respectively: I group is blank group, positive group of II group (diclofenac potassium 10mg/kg/d), III group: be pharmaceutical composition first low dose group (293mg (crude drug)/kg/d), IV group is pharmaceutical composition first high dose group (1172mg (crude drug)/kg/d), V group is pharmaceutical composition second low dose group of the present invention (343mg (crude drug)/kg/d), VI is pharmaceutical composition second high dose group of the present invention (1372mg (crude drug)/kg/d), VII is pharmaceutical composition the third low dose group of the present invention (358mg (crude drug)/kg/d), VIII is pharmaceutical composition the third high dose group of the present invention (1432mg (crude drug)/kg/d).Each is organized mice and all feeds under the same conditions, freely ingest, take the photograph water, room temperature is controlled at 20 ± 1 ℃, and humidity is 60% left and right, gastric infusion is six days continuously, after last administration 60min, the acetum 0.2ml/ of lumbar injection 0.6% only, records mouse writhing number of times (abdominal part indent in 15min, stretch hind leg, buttocks is raised), calculate medicine and the suppression ratio of writhing is passed judgment on to the action effect of pharmaceutical composition, the results are shown in Table 1.

Suppression ratio %=(blank group writhing number of times-administration group writhing number of times)/blank group writhing number of times * 100%

Three kinds of pharmaceutical composition Dichlorodiphenyl Acetates of table 1 cause mouse writhing number of times impact (

n=10)

Note: administration group and the comparison of blank group: ¹p < 0.05, ²p < 0.01; Medicine second, third and medicine first is low, high dose group compares respectively: ³p < 0.05, ⁴p < 0.01.

4. statistical analysis: each organizes mouse writhing mean ± standard deviation for number of times

represent, adopt SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, variance has homogeneous to be checked with LSD, and heterogeneity of variance Dunnett ' s T3 check is carried out comparing between each group, P < 0.05, and P < 0.01 represents that difference has significance.

5. experimental result is as shown in table 1: pharmaceutical composition first low dosage suppression ratio is 10.32%, writhing number of times compares not statistically significant (P > 0.05) with blank group, the suppression ratio of high dose is 33.33%, and writhing number of times relatively has significant difference (P < 0.01) with blank group; Pharmaceutical composition second of the present invention, third suppression ratio low, high dose are respectively 21.83%, 53.10%, 22.42% and 60.47%, and writhing number of times more all has significant difference (P < 0.01) with blank group; Pharmaceutical composition second of the present invention, third is compared and is all had significant difference (P < 0.05 with pharmaceutical composition first high dose group respectively, P < 0.01), pharmaceutical composition second of the present invention, high dose group low with third difference (P > 0.05) of comparing that there are no significant.Sum up, three kinds of pharmaceutical compositions all can suppress the writhing number of times that acetic acid causes mice when low, high dose, pharmaceutical composition second of the present invention, third inhibition are significantly better than pharmaceutical composition first, and the action effect of pharmaceutical composition third of the present invention is slightly better than pharmaceutical composition second of the present invention.

2 three kinds of pharmaceutical compositions of experimental example cause the experimentation of mice pain whipping to thermostimulation

1. medicine and reagent: diclofenac potassium (positive control), Novartis Pharma AG, lot number: X0035;

Pharmaceutical composition first: (preparation method: get 30% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material 334g, merge extractive liquid,, reclaims ethanol to without alcohol taste, continue to add water to extracting liquid volume 5 times, centrifugal, get supernatant concentration to dry, obtain Radix Lamiophlomidis Rotatae extract; Separately get 60% soak with ethanol of 3 times of weight for Aconitum sinomontanum Nakai medical material 400g, emit soak, and continuation ethanol percolation, collect alcoholic solution, and reclaim ethanol, use dissolving with hydrochloric acid residue, centrifugal, get supernatant, add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; The extract of above Radix Lamiophlomidis Rotatae and Aconitum sinomontanum Nakai is mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable tablet.

When evaluating, all establish three kinds of pharmaceutical composition pharmacologically actives high and low dose group; Electronic balance, Haikang, Shanghai Electronic Instruments Plant; Stopwatch, Shenzhen epoch Powerise digital technology Co., Ltd; The water-bath of temperature-controlled, Changzhou Guohua Electric Appliance Co., Ltd., model: HH-501, mouse stomach device, pipettor.

2. animal: Kunming mouse, male and female half and half, body weight 20 ± 2g, is provided the quality certification number by Lanzhou Institute of Biological Products's animal center: the moving word 08-002 of doctor.

3. experimental technique: choose that qualified (mice is immersed the time getting rid of to afterbody in 54-55 ℃ of warm water from tail point, claim TFL, claim again the threshold of pain, with pain threshold, be greater than 10 seconds, be less than 30 seconds for qualified) 80 of healthy mices, be divided at random 8 groups, be: I group is blank group, positive group of II group (diclofenac potassium 10mg/kg/d), III group: be pharmaceutical composition first low dose group of the present invention (293mg (crude drug)/kg/d), IV group is pharmaceutical composition first high dose group of the present invention (1172mg (crude drug)/kg/d), V group is pharmaceutical composition second low dose group of the present invention (343mg (crude drug)/kg/d), VI is pharmaceutical composition second high dose group of the present invention (1372mg (crude drug)/kg/d), VII is pharmaceutical composition the third low dose group of the present invention (358mg (crude drug)/kg/d), VIII is pharmaceutical composition the third high dose group of the present invention (1432mg (crude drug)/kg/d).Before administration, first with stopwatch, measure the pain threshold of respectively organizing mice, and keep a record, gastric infusion is six days continuously, 1h after last gastric infusion, 2h, 4h, 6h measures respectively and respectively organizes mice pain threshold, every time point determining three times is with mean value computation, if TFL surpasses 60 seconds, stops test and calculates according to 60 seconds.After experiment finishes, t check between adding up and organize according to surveyed pain threshold, calculates threshold of pain raising percentage rate, according to the analgesic effect between Comparison of experiment results different pharmaceutical compositions, various dose, the results are shown in Table 2 and table 3.

Percentage rate=(pain threshold after the administration of administration group-blank group pain threshold)/blank group pain threshold * 100% is improved in the threshold of pain

Three kinds of pharmaceutical compositions of table 2 on thermostimulation cause mice pain threshold impact (

n=10)

Note: administration group compares with blank group: ¹p < 0.05, ²p < 0.01.

Percentage rate (n=10) is improved in the threshold of pain that three kinds of pharmaceutical compositions of table 3 cause mice to thermostimulation

4. statistical analysis: each organizes mice mean ± standard deviation for pain threshold represent, adopt SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, when variance has homogeneous, with LSD, check, heterogeneity of variance Dunnett ' s T3 check is carried out comparing between each group, P < 0.05, and P < 0.01 represents that difference has significance.

5. experimental result shows: before drug treating, respectively organize mice pain threshold and the blank group of there was no significant difference of comparing, blank group at 1h, 2h, and 4h, during 6h, mice pain threshold slightly changes but there was no significant difference, as shown in table 2.Raising percentage rate when pharmaceutical composition first low dosage 1h, 2h is respectively 24.56% and 23.53%, pain threshold and the blank group of significant difference of having compared (P < 0.05), 4h, 6h pain threshold and the blank group of there was no significant difference of comparing (P > 0.05), the action effect of high dose group obviously strengthens, pain threshold and the blank group of significant difference of having compared (P < 0.05, P < 0.01), and the threshold of pain is improved percentage rate and is weakened gradually along with the prolongation of time, threshold of pain when pharmaceutical composition second of the present invention, the third low dose group 1h, 2h is improved percentage rate and is respectively 32.46%, 30.25%, 35.09% and 31.93%, with the model group significant difference (P < 0.01) of having compared, and its value reduces gradually along with the prolongation of time, 3h, 4h pain threshold are with the model group significant difference (P < 0.05) of having compared, pharmaceutical composition second of the present invention, the third high dose group pain threshold is with model group statistical significance (the P < 0.05 that compared, P < 0.01), pharmaceutical composition second of the present invention, third is low, high dose group pain threshold is compared respectively, pharmaceutical composition the third analgesic effect of the present invention is omited well but there was no significant difference (P > 0.05), sum up, pharmaceutical composition second of the present invention, third compares with pharmaceutical composition first, analgesic effect significance strengthens, pharmaceutical composition the third analgesic effect of the present invention is slightly better than pharmaceutical composition second of the present invention.

3 three kinds of pharmaceutical compositions of experimental example suppress the experimentation of mice caused by dimethylbenzene xylene auricle edema

1. medicine and reagent: dexamethasone acetate tablets, Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number: 070634; Dimethylbenzene, Xi'an chemical reagent factory, lot number: 010922;

When evaluating, all establish three kinds of pharmaceutical composition pharmacologically actives high and low dose sample; Ordinary electronic balance, Haikang, Shanghai Electronic Instruments Plant; Ten thousand/electronic analytical balance, Beijing Sai Duolisi balance company limited; Microsyringe (0.05ml) Shanghai Medicine Laser Instrument Plant, stopwatch, mouse stomach device, pipettor, card punch (7mm).

2. animal: Kunming mouse, male and female half and half, body weight 20 ± 2g, is provided the quality certification number by Lanzhou Institute of Biological Products's animal center: the moving word 08-0010 of doctor.

3. experimental technique: choose 80 of healthy Kunming mouses, be divided at random 8 groups, every group 10, I group is blank group, III group: pharmaceutical composition first low dose group (293mg (crude drug)/kg/d), IV group is pharmaceutical composition first high dose group (1172mg (crude drug)/kg/d), V group is pharmaceutical composition second low dose group of the present invention (343mg (crude drug)/kg/d), VI is pharmaceutical composition second high dose group of the present invention (1372mg (crude drug)/kg/d), VII is pharmaceutical composition the third low dose group of the present invention (358mg (crude drug)/kg/d), VIII is pharmaceutical composition the third high dose group of the present invention (1432mg (crude drug)/kg/d).Before experiment, respectively organize continuous gastric infusion six days, blank group gives isopyknic purified water, 30min after last gastric infusion, with warm water, by auris dextra scrub, draw 0.03ml/ dimethylbenzene evenly smear and carry out proinflammatory effect on mouse right ear two sides with microsyringe, left ear in contrast, after dripping proinflammatory agent 30min, the dislocation of animal cervical vertebra is put to death, along helix exterior feature, cut left and right auricle, at the same corresponding site of ears, with the card punch of diameter 7mm, sweep away auricle, with analytical balance, weigh.Take two auricle weight differences as swelling level index, each group difference relatively, and obtain and press down swollen rate, the results are shown in Table 4.

Swelling (mg)=auris dextra weight-left ear weight

Press down swollen rate (%)=(model control group swelling-administration group swelling)/model control group swelling * 100%

Three kinds of pharmaceutical composition xylol of table 4 cause mice auricle swelling inhibitory action (

n=10)

Note: with the comparison of blank group: ¹p < 0.05, ²p < 0.01; Medicine second, third low with medicine first, high dose group are compared: ³p < 0.05, ⁴p < 0.01.

4. statistical analysis: each organizes mice auricle swelling mean ± standard deviation for degree

represent, adopt SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, when variance has homogeneous, with LSD, check, heterogeneity of variance Dunnett ' s T3 check is carried out comparing between each group, P < 0.05, and P < 0.01 represents that difference has significance.

5. experimental result as shown in Table 4: three kinds of pharmaceutical compositions all can suppress the swelling of mice caused by dimethylbenzene xylene auricle when low, high dose, have certain antiinflammatory action.Pharmaceutical composition first low dose group is compared with blank group, its suppression ratio is 8.84%, ear swelling degree is with the blank group not statistically significant of comparing, the suppression ratio of high dose group is 38.36%, ear swelling degree and the blank group of significant difference of having compared (P < 0.01); Pharmaceutical composition second of the present invention, third low, high dose group suppression ratio are respectively 19.73%, 47.62%, 22.45% and 52.33%, and ear swelling degree is compared and all had significant difference (P < 0.01) with blank group; Pharmaceutical composition second of the present invention, the third high dose group are compared and are all had significant difference (P < 0.05 with pharmaceutical composition first high dose group respectively, P < 0.01), the ear swelling suppression ratio of pharmaceutical composition third of the present invention higher than pharmaceutical composition second of the present invention but the two is compared not statistically significant.Sum up, pharmaceutical composition second of the present invention, third suppresses mice auricle swelling successful and is better than compositions first, and wherein pharmaceutical composition the third effect of the present invention slightly well.

4 three kinds of pharmaceutical compositions of experimental example suppress the experimentation that carrageenin causes rat paw edema

1. medicine and reagent: dexamethasone acetate tablets, Zhejiang Province XianJu Pharmacy stock Co., Ltd, lot number: 070634; Carrageenin, Sigma company;

When evaluating, all establish three kinds of pharmaceutical composition pharmacologically actives high and low dose group, YLS-7B Mus foot volume measuring apparatus, ordinary electronic balance, Haikang, Shanghai Electronic Instruments Plant; Shaver.

2. animal: SD rat, 80, male and female half and half, body weight 180-220g, is provided by Gansu college of traditional Chinese medicine Experimental Animal Center, the quality certification number: SCXK (sweet) 2008-007.

3. experimental technique: get 80 of healthy SD rats, male and female half and half, 180-220g, be divided at random 8 groups, every group 10, be respectively: I group: blank group, II group: positive controls (dexamethasone 3mg/kg/d), III group: pharmaceutical composition first low dose group (146.5mg (crude drug)/kg/d), IV group is pharmaceutical composition first high dose group (586mg (crude drug)/kg/d), V group is pharmaceutical composition second low dose group of the present invention (171.5mg (crude drug)/kg/d), VI is pharmaceutical composition second high dose group of the present invention (686mg (crude drug)/kg/d), VII is pharmaceutical composition the third low dose group of the present invention (179mg (crude drug)/kg/d), VIII is pharmaceutical composition the third high dose group of the present invention (716mg (crude drug)/kg/d).Before experiment, respectively organize continuous gastric infusion six days, blank group gives isopyknic purified water, before last gastric infusion, with Mus foot volume measuring apparatus, measure the following volume of right ankle joint oiliness writing brush labelling, 30min after administration, first from the subcutaneous upwards injection part in toes middle part with the syringe of No. 26 syringe needles, after turn syringe needle and cause inflammation to the complete 1% carrageenin 0.1ml of hemostasis, respectively at causing 1 after inflammation, 2, 4, within 6 hours, with Mus foot volume measuring apparatus, measure the following volume of right ankle joint oiliness writing brush labelling, the same position of the rat of take difference in volume is swelling degree evaluation index, calculate respectively swelling and suppression ratio, the results are shown in Table 5 and table 6.

The scorching front foot volumetric values of the scorching metapedes volumetric values of swelling (ml)=cause-cause

Suppression ratio (%)=(the average swelling of the average swelling-administration of blank group group)/average swelling * 100% of blank group

Three kinds of pharmaceutical composition on Carrageenan of table 5 cause rat paw edema inhibitory action (

n=10)

Note: with the comparison of blank group: ¹p < 0.05, ²p < 0.01; Pharmaceutical composition second, third is compared with pharmaceutical composition first: ³p < 0.05, ⁴p < 0.01.

Three kinds of pharmaceutical compositions of table 6 cause the suppression ratio (n=10) of rat paw edema to carrageenin processed

4. statistical analysis: each organizes rat paw edema mean ± standard deviation for degree

5. experimental result shows: each administration group before drug treating and the blank group rat paw edema degree there was no significant difference of comparing, after administration, three kinds of each dosage groups of pharmaceutical composition all can suppress rat paw edema, each dosage group swelling of pharmaceutical composition first is with the blank group not statistically significant (P > 0.05) of comparing, pharmaceutical composition first high dose group, pharmaceutical composition second of the present invention, the swelling of pharmaceutical composition third each each time point of dosage group of the present invention is compared with blank group, all there is significant difference (P < 0.01), pharmaceutical composition second low dose group (1h of the present invention, 2h, 6h time point), pharmaceutical composition second high dose group of the present invention, pharmaceutical composition the third low dose group of the present invention, pharmaceutical composition high dose group (1h of the present invention, 2h, 4h time point) ear swelling degree is compared with pharmaceutical composition first, all there is significant difference (P < 0.05, P < 0.01), pharmaceutical composition second of the present invention, pharmaceutical composition of the present invention third foot swelling suppression ratio low, high dose group equi-time point are compared, pharmaceutical composition third of the present invention is slightly high, its swelling there was no significant difference (P > 0.05) of comparing, sum up, pharmaceutical composition second of the present invention, the third antiphlogistic effects are compared with pharmaceutical composition first and are obviously strengthened, and wherein pharmaceutical composition the third antiphlogistic effects of the present invention is slightly better than pharmaceutical composition second of the present invention.

The impact experiment of 5 three kinds of pharmaceutical compositions of experimental example on adjuvant type rats with arthritis immune system (the related immune factor in organ index and serum)

1. medicine and reagent: methotrexate, Shanghai Pharmaceutical's letter friendship pharmacy head factory, lot number 081002; Bacillus calmette-guerin vaccine, 50mg/ props up, institute of biological products, Beijing, lot number 20080104; Liquid paraffin, Xu Dong chemical plant, Beijing, lot number 080606; Anhydrous lanolin, Shi Ying chemical plant, ChangPing, Beijing City, lot number 060601;

When evaluating, three kinds of pharmaceutical composition pharmacologically actives all establish high and low dose group, electronic balance, Haikang, Shanghai Electronic Instruments Plant.

2. animal: wister rat, male, body weight 190～210g,, is provided the quality certification number: SCXK (capital) 2006-0003 by 110 by Beijing dimension tonneau China laboratory animal technology company limited.

3. model is prepared and experimental technique

Get 1 (50mg) of lyophilized bacillus calmette-guerin vaccine element, 80 ℃ of water-bath 1h deactivation, sneak in the mixture 10ml of sterilized liquid paraffin oil and lanoline (2: 1), make Freund ' s Freund's complete adjuvant, in 100 rats (remain 10 and be made as Normal group), right back sufficient sole of the foot intradermal injection 0.1ml causes inflammation, within the 3rd day, rejects the right back sufficient sole of the foot without swelling rat, retains 80, be divided at random 8 groups, 10 every group.It is blank group that experiment is respectively I group, II group is model group, positive group of III group (methotrexate 1mg/kg/d), IV group is pharmaceutical composition first low dose group (293mg (crude drug)/kg/d), V group is pharmaceutical composition first high dose group (1172mg (crude drug)/kg/d), VI group is pharmaceutical composition second low dose group of the present invention (343mg (crude drug)/kg/d), VII group is pharmaceutical composition second high dose group of the present invention (1372mg (crude drug)/kg/d), VIII group is pharmaceutical composition the third low dose group of the present invention (358mg (crude drug)/kg/d), IX group is pharmaceutical composition the third high dose group of the present invention (1432mg (crude drug)/kg/d).Each is organized rat and all feeds under the same conditions, freely ingest, take the photograph water, room temperature is controlled at 20 ± 1 ℃, and humidity is 60% left and right, I, II group are given and normal saline, the III-IX is given and positive drug and pharmaceutical composition of the present invention respectively, and gastric infusion is 30 days continuously, and each is organized rat drug treating and finishes rear 24h broken end blood sampling 4ml, anticoagulant, centrifugal ,-20 ℃ of preservations, measure IL-1 β, TNF-α, PGE ₂, superoxide dismutase (SOD), malonaldehyde (MDA), serum corticosterone, win rat chest gland, spleen and adrenal gland, gravimetry, calculates organ index, the results are shown in Table 7.

4. result is added up: each is organized data and all uses mean ± standard deviation

represent, adopt SPSS10.0 statistical software to carry out date processing.Carry out one factor analysis of variance, variance has homogeneous to be checked with LSD, heterogeneity of variance Dunnett, and s T3 check is carried out comparing between each group, P < 0.05, P < 0.01 represents that difference has significance, between group, relatively with q, checks.

5. experimental result is as shown in the table

Three kinds of pharmaceutical compositions of table 7.1 on the impact of rat chest gland index, index and spleen index, Weight-index (

n=10)

Note: and blank group is relatively: ¹p < 0.05, ²p < 0.01, and model group comparison: ³p < 0.05, ⁴p < 0.01, pharmaceutical composition second, the third high dose group respectively with first comparison, pharmaceutical composition second, the third low dose group respectively with first comparison: ⁵p < 0.05, ⁶p < 0.01.

Three kinds of pharmaceutical compositions of table 7.2 are to IL-1 β, TNF-α, PGE in rat blood serum ₂the impact of content (

n=10)

Three kinds of pharmaceutical compositions of table 7.3 on the impact of SOD, MDA, serum corticosterone in rat blood serum ( n=10)

Note: and blank group is relatively: ¹p < 0.05, ²p < 0.01, and model group comparison: ³p < 0.05, ⁴p < 0.01, pharmaceutical composition second, the third high dose group respectively with the comparison of pharmaceutical composition first, pharmaceutical composition second, the third low dose group respectively with the comparison of pharmaceutical composition first: ⁵p < 0.05, ⁶p < 0.01.

6. experimental result is as table 7.1, 7.2, shown in 7.3 3 tables: pharmaceutical composition first, second, third can obviously reduce the thymus index of rat, index and spleen index, Weight-index, IL-1 β in serum, TNF-α, PGE2, MDA content, wherein, pharmaceutical composition first, second, the correlation factor significant difference (P < 0.05, P < 0.01) of having compared with model group in the third high dose group rat immunity system organ index and serum, pharmaceutical composition second, the third high dose group can reach significance and reduce correlation factor in rat immunity system organ index and serum, compares with pharmaceutical composition first, and action effect is better, wherein IL-1 β in serum, PGE2, the MDA content significant difference (P < 0.05, P < 0.01) of having compared, pharmaceutical composition second, the third high dose group (correlation factor in rat immunity system organ index and serum) is compared with blank group, part serum factor there was no significant difference (P > 0.05), three kinds of pharmaceutical compositions all can raise SOD in rat blood serum, serum corticosterone, compositions second, the third action effect are good compared with compositions first.Sum up, pharmaceutical composition first, second, third all can reduce the relevant organ index of adjuvant arthritis rat immunity system by significance, and correlation factor in serum is all had a certain impact, three kinds of compositions high dose group are compared with positive group, blank group, organ index and part serum factor there was no significant difference (P > 0.05), show that pharmaceutical composition first, second, third pair of RA rat all have certain therapeutical effect, pharmaceutical composition second, the third effect are better than pharmaceutical composition first.

Following embodiment all can realize the effect of above-mentioned experimental example.

The specific embodiment

Embodiment 1: the preparation of capsule

Radix Lamiophlomidis Rotatae 900g, Aconitum sinomontanum Nakai 1300g, Fructus Chebulae's (enucleation) 200g, Radix Aucklandiae 80g, Rhizoma Acori Calami 80g.

Radix Lamiophlomidis Rotatae medical material is boiled 3 times with the decocting of 12,10,8 times of weight respectively, decoct respectively 2,1.5,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 80% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 10, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation) medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Radix Aucklandiae medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; Rhizoma Acori Calami medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Acori Calami extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable capsule.

Embodiment 2: the preparation of granule

Radix Lamiophlomidis Rotatae 250g, Aconitum sinomontanum Nakai 950g, Fructus Chebulae's (enucleation) 35g, Radix Aucklandiae 35g, Rhizoma Acori Calami 25g.

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the superfine powder that particle diameter is 10-40 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable granule.

Embodiment 3: the preparation of injection

Radix Lamiophlomidis Rotatae 550g, Aconitum sinomontanum Nakai 350g, Fructus Chebulae's (enucleation) 95g, Radix Aucklandiae 25g, Rhizoma Acori Calami 35g.

By 40% ethanol extraction of 18 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 9 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 40% ethanolysis absorption, collect 40% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 60% soak with ethanol of 5 times of weight 3 hours for Aconitum sinomontanum Nakai medical material, continue use 60% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 13, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), Rhizoma Acori Calami, the Radix Aucklandiae are mixed in proportion, with the decocting of 12 times of weight, boil 3 times, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable injection.

Embodiment 4: the preparation of spray

Radix Lamiophlomidis Rotatae 110g, Aconitum sinomontanum Nakai 1450g, Fructus Chebulae's (enucleation) 20g, Radix Aucklandiae 95g, Rhizoma Acori Calami 15g.

By 50% ethanol extraction of 15 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 75% soak with ethanol of 5 times of weight 3 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by cation exchange resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 12, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; By 20% ethanol extraction of 5 times of weight 2 times for Fructus Chebulae's (enucleation) medical material, extract 2 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Fructus Chebulae extract that to obtain; By 70% ethanol extraction of 20 times of weight 3 times for Radix Aucklandiae medical material, extract 1.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Radix Aucklandiae extract that to obtain; By the dehydrated alcohol extraction of 10 times of weight 3 times for Rhizoma Acori Calami medical material, extract 0.5 hour at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Acori Calami extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable spray.

Embodiment 5: the preparation of tablet

Radix Lamiophlomidis Rotatae 334g, Aconitum sinomontanum Nakai 400g, Fructus Chebulae's (enucleation) 67g, Radix Aucklandiae 33g, Rhizoma Acori Calami 33g.

Get Aconitum sinomontanum Nakai, Radix Lamiophlomidis Rotatae medical material, after mixing, add twice of 8 times of amount 60% alcohol reflux, each 1.5 hours, merge extractive liquid, filter, it is 1.30-1.35 (60 ℃) that filtrate is concentrated into relative density, add lactose 32g, mix, 70 ℃ following dry, get dry extract, separately get Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae pulverizing medicinal materials becomes fine powder, getting recipe quantity, to become particle diameter with dry cream co-grinding be the superfine powder of 1-40 μ m, add lactose-calcium hydrogen phosphate-pregelatinized Starch (2: 2: 1.5) appropriate, mix, use 92% alcohol granulation, 50 ℃ are dried to half-dried, 40 mesh sieve granulate, granule continues to dry, the Pulvis Talci that adds grain amount 0.5%, 0.3% magnesium stearate mix homogeneously, tabletting, film coating, obtain.

Embodiment 6: the preparation of ointment

Radix Lamiophlomidis Rotatae 950g, Aconitum sinomontanum Nakai 110g, Fructus Chebulae's (enucleation) 15g, Radix Aucklandiae 15g, Rhizoma Acori Calami 95g.

By 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 90% soak with ethanol of 2 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-600 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 11, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation) medical material is boiled 2 times with the decocting of 15 times of weight, decoct 2.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; By 50% ethanol extraction of 18 times of weight 3 times for Radix Aucklandiae medical material, extract 2 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Radix Aucklandiae extract that to obtain; Rhizoma Acori Calami medical material is boiled 2 times with the decocting of 8 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Rhizoma Acori Calami extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable ointment.

Embodiment 7: the preparation of soft capsule

Radix Lamiophlomidis Rotatae 210g, Aconitum sinomontanum Nakai 320g, Fructus Chebulae's (enucleation) 35g, Radix Aucklandiae 25g, Rhizoma Acori Calami 25g.

Radix Lamiophlomidis Rotatae medical material is boiled 3 times with the decocting of 8 times of weight respectively, decoct respectively 1 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 100% soak with ethanol of 4 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 100% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 10, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the micropowder that particle diameter is 1-40 μ m, be mixed in proportion to obtain admixed finepowder; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, admixed finepowder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable soft capsule.

Embodiment 8: the preparation of powder

Radix Lamiophlomidis Rotatae 550g, Aconitum sinomontanum Nakai 800g, Fructus Chebulae's (enucleation) 255g, Radix Aucklandiae 55g, Rhizoma Acori Calami 55g.

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 100-150 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable powder.

Embodiment 9: the preparation of patch

Radix Lamiophlomidis Rotatae 110g, Aconitum sinomontanum Nakai 110g, Fructus Chebulae's (enucleation) 15g, Radix Aucklandiae 10g, Rhizoma Acori Calami 10g.

Radix Lamiophlomidis Rotatae medical material is boiled 1 time with the decocting of 20 times of weight respectively, decoct 2 hours, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 95% soak with ethanol of 2 times of weight 3 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-450 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 12, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), Rhizoma Acori Calami, the Radix Aucklandiae are mixed in proportion, with 70% ethanol extraction of 12 times of weight 3 times, extract 2.5 hours at every turn, collect, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable patch.

Embodiment 10: the preparation of tablet

Get Aconitum sinomontanum Nakai, Radix Lamiophlomidis Rotatae medical material, after mixing, add twice of 8 times of amount 60% alcohol reflux, each 1.5 hours, merge extractive liquid, filter, it is 1.30-1.35 (60 ℃) that filtrate is concentrated into relative density, add lactose 32g, mix, 70 ℃ following dry, get dry extract, separately get Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae pulverizing medicinal materials becomes fine powder, get recipe quantity and dry cream mixed powder is broken into fine powder, add lactose-calcium hydrogen phosphate-pregelatinized Starch (2: 2: 1.5) appropriate, mix, use 92% alcohol granulation, 50 ℃ are dried to half-dried, 40 mesh sieve granulate, granule continues to dry, the Pulvis Talci that adds grain amount 0.5%, 0.3% magnesium stearate mix homogeneously, tabletting, film coating, obtain tablet.

Embodiment 11: the preparation of powder

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 60% alcohol reflux 2 times of 8 times of weight, each 1.5 hours, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract, separately gets Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material and is ground into respectively fine powder, is mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable powder.

Embodiment 12: the preparation of aerosol

Radix Lamiophlomidis Rotatae 550g, Aconitum sinomontanum Nakai 950g, Fructus Chebulae's (enucleation) 95g, Radix Aucklandiae 35g, Rhizoma Acori Calami 35g.

Radix Lamiophlomidis Rotatae medical material is boiled 2 times with the decocting of 12,10 times of weight respectively, decoct respectively 1.5,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 85% soak with ethanol of 4 times of weight 5 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-600 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; By the dehydrated alcohol extraction of 5 times of weight 3 times for Fructus Chebulae's (enucleation) medical material, extract 2.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Fructus Chebulae extract that to obtain; Radix Aucklandiae medical material is boiled 3 times with the decocting of 18 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; By 50% ethanol extraction of 15 times of weight 3 times for Rhizoma Acori Calami medical material, extract 2.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Acori Calami extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable aerosol.

Embodiment 13: the preparation of soft capsule

Radix Lamiophlomidis Rotatae medical material is boiled 3 times with the decocting of 8 times of weight respectively, decoct respectively 1 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 100% soak with ethanol of 4 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 100% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 10, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into fine powder, be mixed in proportion to obtain mixing fine powders; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixing fine powders are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable soft capsule.

Embodiment 14: the preparation of drop pill

Radix Lamiophlomidis Rotatae 110g, Aconitum sinomontanum Nakai 1100g, Fructus Chebulae's (enucleation) 15g, Radix Aucklandiae 15g, Rhizoma Acori Calami 15g.

By 60% ethanol extraction of 10 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 1.5 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 9 times of extracting liquid volume, centrifugal, get supernatant by HPD-450 macroporous adsorbent resin, first wash with water to effluent colourless, again with 60% ethanolysis absorption, collect 60% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 75% soak with ethanol of 2 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 75% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 12, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), Rhizoma Acori Calami, the Radix Aucklandiae are mixed in proportion, with the decocting of 20 times of weight, boil 3 times, decoct respectively 1.5,1,0.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable drop pill.

Embodiment 15: the preparation of tablet

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 40-100 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable tablet.

Embodiment 16: the preparation of quick releasing formulation

Radix Lamiophlomidis Rotatae 890g, Aconitum sinomontanum Nakai 1100g, Fructus Chebulae's (enucleation) 395g, Radix Aucklandiae 85g, Rhizoma Acori Calami 85g.

Radix Lamiophlomidis Rotatae medical material is boiled 2 times with the decocting of 10 times of weight respectively, decoct respectively 2.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 80% soak with ethanol of 3 times of weight 3 hours for Aconitum sinomontanum Nakai medical material, continue use 80% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 12, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 100-150 μ m, be mixed in proportion to obtain mixed powder; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable quick releasing formulation.

Embodiment 17: the preparation of pill

Radix Lamiophlomidis Rotatae 400g, Aconitum sinomontanum Nakai 650g, Fructus Chebulae's (enucleation) 65g, Radix Aucklandiae 20g, Rhizoma Acori Calami 20g.

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 60% alcohol reflux 2 times of 8 times of weight, each 1.5 hours, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract, separately gets Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material and is ground into respectively fine powder, is mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add polyvinylpyrrolidone and dehydrated alcohol appropriate, according to common process, make clinical or pharmaceutically acceptable pill.

Embodiment 18: the preparation of oral liquid

By 70% ethanol extraction of 10 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 1 hour, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 5 times of extracting liquid volume, centrifugal, get supernatant by HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with 70% ethanolysis absorption, collect 70% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-300 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami medical material are mixed in proportion, with the decocting of 5 times of weight, boil 3 times, decoct respectively 1.5,1,0.5 hours at every turn, collection, merge extractive liquid,, concentrated, dry that mixed extract mixes Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, admixed finepowder, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable oral liquid.

Embodiment 19: the preparation of granule

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, add 50% alcohol reflux 3 times of 12 times of weight after mixing, each 0.5 hour, merge extractive liquid,, filtered, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material and be ground into respectively fine powder, be mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add lactose-pregelatinized Starch (2: 3) 320g, mix, use 95% alcohol granulation, 50 ℃ are dried to half-driedly, and 40 mesh sieve granulate, obtain granule.

Embodiment 20: the preparation of slow releasing preparation

Radix Lamiophlomidis Rotatae 550g, Aconitum sinomontanum Nakai 800g, Fructus Chebulae's (enucleation) 255g, Radix Aucklandiae 55g, Rhizoma Acori Calami 55g

Radix Lamiophlomidis Rotatae medical material is boiled 2 times with the decocting of 12 times of weight respectively, decoct respectively 2,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 70% soak with ethanol of 1 times of weight 4 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-600 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 11, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), Rhizoma Acori Calami, Radix Aucklandiae medical material are mixed in proportion, with 50% ethanol extraction of 15 times of weight 2 times, extract 1.5 hours at every turn, collect, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable slow releasing preparation.

Embodiment 21: the preparation of liniment

By 5 50% ethanol extraction 3 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 80% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 10, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), Rhizoma Acori Calami, the Radix Aucklandiae are mixed in proportion, with 70% ethanol extraction of 12 times of weight 3 times, extract 2.5 hours at every turn, collect, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable liniment.

Embodiment 22: the preparation of ointment

Radix Lamiophlomidis Rotatae 615g, Aconitum sinomontanum Nakai 1100g, Fructus Chebulae's (enucleation) 125g, Radix Aucklandiae 50g, Rhizoma Acori Calami 50g

By 20% ethanol extraction of 5 times of weight 3 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 15 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 20% ethanolysis absorption, collect 20% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 80% soak with ethanol of 2 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by cation exchange resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 11, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation) medical material is boiled 2 times with the decocting of 8 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Radix Aucklandiae medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; By 70% ethanol extraction of 18 times of weight 2 times for Rhizoma Acori Calami medical material, extract 1.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Acori Calami extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable ointment.

Embodiment 23: the preparation of effervescent tablet

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 40-180 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable effervescent tablet.

Embodiment 24: the preparation of patch

Radix Lamiophlomidis Rotatae 780g, Aconitum sinomontanum Nakai 1200g, Fructus Chebulae's (enucleation) 260g, Radix Aucklandiae 65g, Rhizoma Acori Calami 65g, Rhizoma Corydalis 90g.

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material, 70% alcohol reflux 2 times that adds 5 times of weight after mixing, each 2 hours, collection, merge extractive liquid,, filter, and reclaims ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable patch.

Embodiment 25: the preparation of tablet

Radix Lamiophlomidis Rotatae 334g, Aconitum sinomontanum Nakai 400g, Fructus Chebulae's (enucleation) 67g, Radix Aucklandiae 33g, Rhizoma Acori Calami 33g, Rhizoma Corydalis 42g.

Get Aconitum sinomontanum Nakai, Radix Lamiophlomidis Rotatae medical material, after mixing, add twice of 8 times of amount 60% alcohol reflux, each 1.5 hours, merge extractive liquid, filter, it is 1.30-1.35 (60 ℃) that filtrate is concentrated into relative density, add lactose 60g, mix, 70 ℃ following dry, get dry extract, separately get Fructus Chebulae's (enucleation), Rhizoma Acori Calami, the Radix Aucklandiae, corydalis tuber medicinal material is ground into fine powder, get recipe quantity and dry cream mixed powder is broken into fine powder, add lactose-calcium hydrogen phosphate-pregelatinized Starch (2: 2: 1.5) 100g, mix, use 92% alcohol granulation, 50 ℃ are dried to half-dried, 40 mesh sieve granulate, granule continues to dry, the Pulvis Talci that adds grain amount 0.5%, 0.3% magnesium stearate mix homogeneously, tabletting, film coating, obtain tablet.

Embodiment 26: the preparation of ointment

Radix Lamiophlomidis Rotatae 900g, Aconitum sinomontanum Nakai 1300g, Fructus Chebulae's (enucleation) 200g, Radix Aucklandiae 80g, Rhizoma Acori Calami 80g, Rhizoma Corydalis 90g.

Radix Lamiophlomidis Rotatae medical material is boiled 2 times with the decocting of 10,8 times of weight respectively, decoct respectively 2,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by HPD-300 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the decocting of 12 times of weight, boil 3 times, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable ointment.

Embodiment 27: the preparation of capsule

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, Rhizoma Corydalis are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 40-100 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable capsule.

Embodiment 28: the preparation of quick releasing formulation

Radix Lamiophlomidis Rotatae 890g, Aconitum sinomontanum Nakai 1100g, Fructus Chebulae's (enucleation) 390g, Radix Aucklandiae 45g, Rhizoma Acori Calami 85g, Rhizoma Corydalis 85g.

By 70% ethanol extraction of 10 times of weight 3 times for Radix Lamiophlomidis Rotatae medical material, each extraction 1.5 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 7 times of extracting liquid volume, centrifugal, get supernatant by HPD-100 macroporous adsorbent resin, first wash with water to effluent colourless, again with 70% ethanolysis absorption, collect 70% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 95% soak with ethanol of 5 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, continue use 95% ethanol percolation, collect percolate, concentrated, hydro-oxidation sodium is adjusted PH to 11, standing centrifugal, and precipitation is washed to neutrality, is dried and obtains Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 10-100 μ m, be mixed in proportion to obtain mixed powder; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable quick releasing formulation.

Embodiment 29: the preparation of granule

Radix Lamiophlomidis Rotatae 550g, Aconitum sinomontanum Nakai 350g, Fructus Chebulae's (enucleation) 95g, Radix Aucklandiae 25g, Rhizoma Acori Calami 35g, Rhizoma Corydalis 45g.

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae's (enucleation), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material, 70% alcohol reflux 2 times that adds 5 times of weight after mixing, each 2 hours, collection, merge extractive liquid,, filter, and reclaims ethanol extremely without alcohol taste, be dried to obtain mixed extract, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable granule.

Embodiment 30: the preparation of capsule

Radix Lamiophlomidis Rotatae 620g, Aconitum sinomontanum Nakai 1300g, Fructus Chebulae's (enucleation) 135g, Radix Aucklandiae 85g, Rhizoma Acori Calami 45g, Rhizoma Corydalis 95g.

By 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by cation exchange resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract; By 80% soak with ethanol of 3 times of weight 2 hours for Aconitum sinomontanum Nakai medical material, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by cation exchange resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 10, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract; Fructus Chebulae's (enucleation) medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Radix Aucklandiae medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Aucklandiae extract that to obtain; By 70% ethanol extraction of 12 times of weight 3 times for Rhizoma Acori Calami medical material, extract 2.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Acori Calami extract that to obtain; By 70% ethanol extraction of 12 times of weight 3 times for corydalis tuber medicinal material, extract 2.5 hours at every turn, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Rhizoma Corydalis extract that to obtain; Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract, Rhizoma Acori Calami extract and Rhizoma Corydalis extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable capsule.

Claims

1. anti-inflammatory and antalgic or an anti inflammatory immunity pharmaceutical composition, is characterized in that the crude drug of this pharmaceutical composition consists of:

Radix Lamiophlomidis Rotatae 100-1000 weight portion, Aconitum sinomontanum Nakai 100-1500 weight portion, Fructus Chebulae 10-500 weight portion, Radix Aucklandiae 10-100 weight portion, Rhizoma Acori Calami 10-100 weight portion.

2. pharmaceutical composition as claimed in claim 1, is characterized in that the crude drug of this pharmaceutical composition consists of:

Radix Lamiophlomidis Rotatae 200-600 weight portion, Aconitum sinomontanum Nakai 300-1000 weight portion, Fructus Chebulae 30-100 weight portion, Radix Aucklandiae 20-40 weight portion, Rhizoma Acori Calami 20-40 weight portion.

3. pharmaceutical composition as claimed in claim 2, is characterized in that the crude drug of this pharmaceutical composition consists of:

Radix Lamiophlomidis Rotatae 900 weight portions, Aconitum sinomontanum Nakai 1300 weight portions, Fructus Chebulae (pitted) 200 weight portions, the Radix Aucklandiae 80 weight portions, Rhizoma Acori Calami 80 weight portions.

4. pharmaceutical composition as claimed in claim 2, is characterized in that the crude drug of this pharmaceutical composition consists of:

Radix Lamiophlomidis Rotatae 334 weight portions, Aconitum sinomontanum Nakai 400 weight portions, Fructus Chebulae (pitted) 67 weight portions, the Radix Aucklandiae 33 weight portions, Rhizoma Acori Calami 33 weight portions.

5. any one pharmaceutical composition as described in claim 1-4, is characterized in that adding Rhizoma Corydalis 30-100 weight portion.

6. the preparation method of any one pharmaceutical composition as described in claim 1-5, is characterized in that the method is:

Pharmaceutical composition crude drug of the present invention adds conventional adjuvant, according to common process, makes clinical or pharmaceutically acceptable dosage form.

7. the preparation method of any one pharmaceutical composition as described in claim 1-4, is characterized in that the method is:

Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20%-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

50%-100% soak with ethanol 1-5 hour by Aconitum sinomontanum Nakai medical material by 1-5 times of weight, continuation 50%-100% ethanol percolation, collects percolate, concentrated, and hydro-oxidation sodium is adjusted PH to 8-13, standing, centrifugal, and precipitation is washed to neutrality, is dried to obtain Aconitum sinomontanum Nakai extract; Or by the 50%-100% soak with ethanol 1-5 hour of Aconitum sinomontanum Nakai medical material use 1-5 times of weight, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, and use dissolving with hydrochloric acid residue, centrifugal, get supernatant, supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, is first washed with water to effluent colourlessly, then adsorb with ethanolysis, collect alcoholic solution, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 7-14, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract;

Fructus Chebulae (pitted) medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Or by the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time for Fructus Chebulae (pitted) medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, be dried and obtain Fructus Chebulae extract;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, Fructus Chebulae extract, Radix Aucklandiae extract and Rhizoma Acori Calami extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

8. preparation method as claimed in claim 7, is characterized in that the method is:

Radix Lamiophlomidis Rotatae medical material is boiled 3 times with the decocting of 12,10,8 times of weight respectively, decoct respectively 2,1.5,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae (pitted) medical material is boiled 3 times with the decocting of 12 times of weight, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Fructus Chebulae extract that to obtain; Or by 70% ethanol extraction of 12 times of weight 3 times for Fructus Chebulae (pitted) medical material, extract 2.5 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, the dry Fructus Chebulae extract that to obtain; at every turn

9. the preparation method of any one pharmaceutical composition as described in claim 1-5, is characterized in that the method is:

Radix Lamiophlomidis Rotatae medical material is boiled 1-3 time with the decocting of 5-20 times of weight, decoct 0.5-3 hour at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, extract 0.5-10 hour at every turn, and collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, be dried and obtain Radix Lamiophlomidis Rotatae extract; Or by the 20%-70% ethanol extraction of 5-20 times of weight 1-3 time for Radix Lamiophlomidis Rotatae medical material, each 0.5-10 hour that extracts, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, continue to add water to 8-20 times of volume, centrifugal, get supernatant by cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, again with the absorption of 20-70% ethanolysis, collect ethanol, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the decocting of 5-20 times of weight, boil 1-3 time, decoct 0.5-5 hour at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or by Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the 20%-dehydrated alcohol extraction of 5-20 times of weight 1-3 time, extract 0.5-10 hour at every turn, collect, merge extractive liquid,, concentrated, be dried to obtain mixed extract;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract and mixed extract are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

10. preparation method as claimed in claim 9, is characterized in that the method is:

Radix Lamiophlomidis Rotatae medical material is boiled 3 times with the decocting of 12,10,8 times of weight respectively, decoct respectively 2,1.5,1 hours at every turn, collection, merge extractive liquid,, concentrated, the dry Radix Lamiophlomidis Rotatae extract that to obtain; Or by 12 50% ethanol extraction 3 times for Radix Lamiophlomidis Rotatae medical material, extract 2 hours, collect, merge extractive liquid,, reclaim ethanol to without alcohol taste, add water centrifugal, get supernatant concentration to dry, the dry Radix Lamiophlomidis Rotatae extract that to obtain; at every turn Or by 50% ethanol extraction of 12 times of weight 2 times for Radix Lamiophlomidis Rotatae medical material, each extraction 2 hours, collection, merge extractive liquid,, reclaim ethanol extremely without alcohol taste, add water to 10 times of extracting liquid volume, centrifugal, get supernatant by HPD-300 macroporous adsorbent resin, first wash with water to effluent colourless, again with 50% ethanolysis absorption, collect 50% alcoholic solution, reclaim ethanol, be dried to obtain Radix Lamiophlomidis Rotatae extract;

Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are mixed in proportion, with the decocting of 12 times of weight, boil 3 times, decoct 1.5 hours at every turn, collection, merge extractive liquid,, concentrated, the dry mixed extract that to obtain; Or by Fructus Chebulae (pitted), Rhizoma Acori Calami, Radix Aucklandiae medical material or Fructus Chebulae (pitted), Rhizoma Acori Calami, the Radix Aucklandiae, corydalis tuber medicinal material are mixed in proportion, with 70% ethanol extraction of 12 times of weight 3 times, extract 2.5 hours at every turn, collect, merge extractive liquid,, concentrated, be dried to obtain mixed extract;

The preparation method of 11. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

50%-100% soak with ethanol 1-5 hour by Aconitum sinomontanum Nakai medical material by 1-5 times of weight, continuation 50%-100% ethanol percolation, collects percolate, concentrated, and hydro-oxidation sodium is adjusted PH to 8-13, standing, centrifugal, and precipitation is washed to neutrality, is dried to obtain Aconitum sinomontanum Nakai extract; Or by the 50%-100% soak with ethanol 1-5 hour of Aconitum sinomontanum Nakai medical material use 1-5 times of weight, emit soak, and continue with ethanol percolation to inanimate object alkali reaction, collect alcoholic solution, and reclaim ethanol, use dissolving with hydrochloric acid residue, centrifugal, get supernatant, add ammonia spirit, supernatant is passed through to cation exchange resin or HPD-300 or HPD-100 or HPD-450 or HPD-600 macroporous adsorbent resin, first wash with water to effluent colourless, with ethanolysis absorption, collect alcoholic solution again, reclaim ethanol; Reclaim liquid and add ammonia spirit, regulate after pH value 7-14, with chloroform extraction, merge chloroform soln, be recycled to dryly, obtain Aconitum sinomontanum Nakai extract;

Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into fine powder, be mixed in proportion to obtain mixing fine powders;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixing fine powders are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

12. preparation methoies as claimed in claim 11, is characterized in that the method is:

Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 1-180 μ m, be mixed in proportion to obtain mixed powder;

Radix Lamiophlomidis Rotatae extract, Aconitum sinomontanum Nakai extract, mixed powder are mixed, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

13. preparation methoies as claimed in claim 11, is characterized in that the method is:

Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaning, drying, be ground into the medicated powder that particle diameter is 10-100 μ m, be mixed in proportion to obtain mixed powder;

The preparation method of 14. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material is ground into respectively fine powder, is mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

The preparation method of 15. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material is ground into respectively the medicated powder that particle diameter is 40-180 μ m, is mixed to get mixed powder; By above dry cream and mixed powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

The preparation method of 16. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 20%-100% alcohol reflux 1-3 time of 5-12 times of weight, each 0.5-5 hour, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract; Separately get Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material is ground into respectively the superfine powder that particle diameter is 1-40 μ m, is mixed to get mixing superfine powder; By above dry cream and mixing superfine powder mix homogeneously, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

17. preparation methoies as claimed in claim 14, is characterized in that the method is:

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, 60% alcohol reflux 2 times that adds 8 times of weight after mixing, each 1.5 hours, merge extractive liquid,, filter, filtrate is concentrated, dry getting dry extract, separately get Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material is ground into respectively fine powder, is mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

18. preparation methoies as claimed in claim 17, is characterized in that the method is:

Get Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai medical material, after mixing, add 60% alcohol reflux 2 times of 8 times of weight, each 1.5 hours, merge extractive liquid,, filters, and filtrate is concentrated, dry getting dry extract, separately gets Fructus Chebulae (pitted), Rhizoma Acori Calami, Radix Aucklandiae medical material and is ground into respectively fine powder, is mixed to get mixing fine powders; By above dry cream and mixing fine powders mix homogeneously, add conventional adjuvant, according to common process, make tablet.

The preparation method of 19. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into fine powder, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

The preparation method of 20. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into the medicated powder that particle diameter is 40-180 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

The preparation method of 21. any one pharmaceutical compositions as described in claim 1-5, is characterized in that the method is:

Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami medical material or Radix Lamiophlomidis Rotatae, Aconitum sinomontanum Nakai, Fructus Chebulae (pitted), the Radix Aucklandiae, Rhizoma Acori Calami, corydalis tuber medicinal material are carried out respectively to remove impurity, cleaned, dry, be ground into the superfine powder that particle diameter is 1-40 μ m, be mixed in proportion, add conventional adjuvant, according to common process, make clinical or pharmaceutically acceptable dosage form.

The preparation method of 22. any one pharmaceutical compositions described in claim 6-17 or 19-21, is characterized in that: wherein said clinical or pharmaceutically acceptable dosage form refers to tablet, capsule, powder, pill, granule, soft extract with bee honey agent, oral liquid formulations, ejection preparation or external preparation.

23. preparation methoies as claimed in claim 22, is characterized in that: described pill is drop pill or honeyed pill.

The preparation method of 24. any one pharmaceutical compositions described in claim 6-17 or 19-21, is characterized in that: wherein said clinical or pharmaceutically acceptable dosage form is slow releasing preparation, quick releasing formulation or controlled release preparation.

The application of 25. any one pharmaceutical compositions as described in claim 1-5 in preparing anti-inflammation analgesis medicament.

The application of 26. any one pharmaceutical compositions as described in claim 1-5 in preparing anti inflammatory immunity medicine.

The application of 27. any one pharmaceutical compositions as described in claim 1-5 in preparation treatment soft tissue injury medicine.

Application in the skeletal muscle arthralgia that 28. any one pharmaceutical compositions as described in claim 1-5 cause in preparation treatment osteoarthritis, swelling, joint stuffiness medicine.

The application of 29. any one pharmaceutical compositions as described in claim 1-5 in preparing drugs for rheumatoid arthritis.