CN101825528B - Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection - Google Patents
Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection Download PDFInfo
- Publication number
- CN101825528B CN101825528B CN2010101540973A CN201010154097A CN101825528B CN 101825528 B CN101825528 B CN 101825528B CN 2010101540973 A CN2010101540973 A CN 2010101540973A CN 201010154097 A CN201010154097 A CN 201010154097A CN 101825528 B CN101825528 B CN 101825528B
- Authority
- CN
- China
- Prior art keywords
- trapping
- smoke
- capture
- cigarette
- flue gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to a three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection, which belongs to the technical field of cigarette smoke safety evaluation. The three-stage trapping method comprises the following steps: first-stage trapping: Cambridge filter trapping, second-stage trapping: PBS (polybutadiene styrene) solution trapping and third-stage trapping: organic solvent trapping, wherein in the second-stage trapping of the cigarette mainstream smoke, the PBS solution the pH value of which is 7.4 is adopted to trap the water-soluble components of the gas phase matter of the mainstream smoke, and in the third-stage trapping of the cigarette mainstream smoke, 70 percent of glycerol is adopted to trap the organic phase components of the gas phase matter of the mainstream smoke. The invention has the advantages of improving the trapping grade of the cigarette mainstream smoke, increasing the trapping comprehensiveness and the trapping efficiency of the smoke, promoting the accuracy and the scientificity of the smoke toxicity detection and the like.
Description
Technical field:
The present invention relates to a kind of three-stage trapping method that is used for the cigarette mainstream flue gas of flue gas toxity detection, belong to cigarette smoke safety evaluatio technical field.
Background technology:
The safety evaluatio of cigarette and cigarette additive is intended to promote the quality and the level of cigarette, and the consumer is responsible for.The capture method of cigarette mainstream flue gas has cambridge filter, electrostatic precipitation, injection bump, cold-trap, solid absorption and solvent to capture.Wherein, the application that cambridge filter captures and solvent captures is more extensive, and the capture effect is more satisfactory.Cambridge filter is used to capture the granule phase substance part of cigarette mainstream flue gas usually; The solution drip catcher is commonly used to capture the gas phase thing part of main flume.
At present, the secondary capture method is adopted in the capture of the cigarette mainstream flue gas of the current international practice usually, promptly adopts cambridge filter as the 1st grade of capture, is used to capture the granule phase substance part of cigarette mainstream flue gas; Connecting a solution drip catcher as the 2nd grade of capture thereafter, be used to capture the gas phase thing part of cigarette mainstream flue gas.What the flue gas trapping method that is used for the cigarette smoke cytotoxicity detection that official of Her Majesty the Queen in right of Canada as represented by the minister of Healt is recommended adopted promptly is the secondary capture method, and wherein the 2nd grade of used solution of capture is phosphate buffer (PBS solution).The cigarette smoke gas phase thing that PBS solution captures partly is a water soluble ingredient, and cigarette mainstream flue gas gas phase thing part complicated component removes water soluble ingredient, also has water insoluble ingredients.Different solutions and pH value thereof exist than big-difference the arresting efficiency and the capture composition of main flume gas phase part.Existing cigarette smoke capture method has been ignored the influence of the pH value of the capture of water insoluble ingredients of cigarette smoke gas phase thing and solution to flue gas trapping efficient.
Dimethyl diaminophenazine chloride cytotoxicity method is widely used for the cytotoxicity of evaluating cigarette flue gas and chemicals by domestic and international laboratory, this detection method is applicable to and detects other arresting efficiency of the different capture level of cigarette mainstream flue gas, and the pH value of solution is to the influence of flue gas trapping efficient.
At present, about the existing report of the research of the capture mode of cigarette mainstream flue gas.But, do not find open report with content same document of the present invention through the document search.
[1] Wang Qinmei, Lan Qinxian, Wu Jiatong etc., the homemade Virginian-type cigarette smoke condensate of 2007. parts
The dimethyl diaminophenazine chloride cell toxicity test. tobacco science and technology, 5:41-43.
[2]Health?Canada?Offical?Method?T-502，second?edition，2004.Neutral?reduptake?assay?for?mainstream?tobacco?smoke.
Summary of the invention:
The objective of the invention is to overcome the deficiency of prior art, and a kind of three-stage trapping method that is used for the cigarette mainstream flue gas that flue gas toxity detects is provided, to improve the comprehensive and arresting efficiency that cigarette smoke captures, for the safety evaluatio that comprehensively, accurately, scientifically carries out cigarette and cigarette additive provides technical support.
The present invention improves to obtain on the basis of existing technology.The present invention captures by the 3rd level of the cigarette mainstream flue gas of increase, captures the water insoluble ingredients of cigarette smoke gas phase thing; In the 3rd level of cigarette smoke captures, utilize 70% glycerine to carry out the capture of cigarette smoke gas phase thing organic phase composition simultaneously, improved the arresting efficiency of cigarette smoke; In the 2nd grade of capture, improved the arresting efficiency of the water soluble ingredient of cigarette smoke gas phase thing by the pH value of optimizing PBS solution to cigarette smoke.
The Chinese hamster ovary cell that the present invention relates to (Chinese Hamster Ovary Cell, Chinese hamster ovary celI) is laboratory normal experiment cell, buys from cell preservation both domestic and external mechanism to obtain.
Method of the present invention comprises that the 1st grade of capture-cambridge filter captures, the 2nd grade of capture-PBS solution captures and the capture of 3rd level capture-organic solvent; Wherein:
In the 2nd grade of capture of cigarette mainstream flue gas, PBS solution adopts the pH value of optimizing 7.4, carries out the capture of mainstream smoke gas phase object water soluble ingredient.
In the 3rd level of cigarette mainstream flue gas captures, select for use 70% glycerine to carry out the capture of mainstream smoke gas phase object organic phase composition.
The present invention has has increased the capture of cigarette mainstream flue gas rank, improved advantages such as the comprehensive and arresting efficiency of flue gas trapping, the accuracy that has promoted the flue gas toxity detection and science.
Description of drawings:
The flue gas sample that Fig. 1 is captured for the three-stage trapping mode compares the toxic action of Chinese hamster ovary celI
Fig. 2 is the flue gas sample that captures of the PBS solution of different pH values to the toxic action of Chinese hamster ovary celI relatively
Fig. 3 is the toxic action comparison of the flue gas sample of 3 kinds of organic solvents captures to Chinese hamster ovary celI
Embodiment:
Below be embodiments of the invention, but content of the present invention is not limited thereto.
Embodiment one: the three-stage trapping mode of cigarette mainstream flue gas
According to a conventional method, on semi-automatic turntable type smoking machine (Borgwaldt RM20/cs), carry out the connection of drip catcher.The 1st grade of cambridge filter captures and adopts the filter disc of 92mm to carry out; After the 1st grade of cambridge filter captures, connect a glass solution drip catcher that places in the ice-water bath, hold the PBS solution of 15mL in it, the pH value is 7.2; Connecting one with the 2nd grade of PBS solution drip catcher places glass solution drip catcher in the ice-water bath, holds the ethyl acetate of 15mL in it, captures as 3rd level.Instrument state detects and finishes, and according to a conventional method, carries out the suction of 20 Cigarettes and the capture of cigarette mainstream flue gas.
Flue gas is collected and is finished, and cambridge filter soaks with dimethyl sulfoxide (DMSO) (DMSO), and ultrasonic 20 minutes, extract TPM (TPM), be settled to 10mg TPM/mL DMSO; According to the amount of granule phase substance, the volume that solution is captured part (the 2nd grade PBS solution trapping solution and 3rd level ethyl acetate trapping solution) is settled at last and is equivalent to 10mg TPM/mL solution.
With reference to the method [2] of Her Majesty the Queen in right of Canada as represented by the minister of Healt, the flue gas sample for preparing is carried out the dimethyl diaminophenazine chloride cytotoxicity detect, cell line is Chinese hamster ovary cell (Chinese hamster ovary celI).The result shows (Fig. 1), and after cambridge filter capture of cigarette mainstream flue gas process and PBS solution captured, the 3rd level trapping solution pair cell that carries out with ethyl acetate still had certain toxic action.In cytotoxicity detected used volume range, ethyl acetate did not have toxic action (table 1) to Chinese hamster ovary celI.Therefore, for carry out the safety evaluatio of cigarette smoke comprehensive, scientifically, be necessary to carry out the capture of the 3rd level flue gas gas phase thing organic phase composition of cigarette mainstream flue gas.
Table 1:3 kind organic solvent compares the inhibiting rate of Chinese hamster ovary celI
Two: the 2 grades of pH values that capture used PBS solution of embodiment are optimized
Method by embodiment one is carried out, and selects for use the PBS solution (pH 7.0,7.2,7.4) of 3 pH values to carry out the capture of cigarette mainstream flue gas gas phase thing water soluble ingredient in the 2nd grade of capture.
The result shows (Fig. 2), and the flue gas sample that the PBS solution of pH 7.4 is captured in 3 pH values is the strongest to the toxic action of Chinese hamster ovary celI, and therefore, the PBS solution of pH 7.4 is the highest to the arresting efficiency of cigarette mainstream flue gas gas phase thing water soluble ingredient.
Embodiment captured the selection of used organic solvent in three: the 3
Method by embodiment one is carried out, and 3rd level is selected 3 kinds of organic solvents for use in capturing: ethyl acetate, 70% glycerine and acetone carry out the capture of cigarette mainstream flue gas gas phase thing organic phase composition.
The result shows (Fig. 3), and the flue gas sample that 70% glycerine is captured in 3 kinds of organic solvents is to the toxic action maximum of Chinese hamster ovary celI.In cytotoxicity detects used volume range, in 3 kinds of organic solvents 70% glycerine to the toxic action of Chinese hamster ovary celI a little less than, acetone is to the toxic action maximum (table 1) of Chinese hamster ovary celI.Therefore, 70% glycerine is applicable to the capture of carrying out cigarette mainstream flue gas gas phase thing organic phase.
Claims (1)
1. a three-stage trapping method that is used for the cigarette mainstream flue gas of flue gas toxity detection comprises that the 1st grade of capture-cambridge filter captures, the 2nd grade of capture-PBS solution captures, and it is characterized in that also comprising that 3rd level capture-organic solvent captures; Wherein:
A. in the 2nd of cigarette mainstream flue gas the grade of capture, the pH value that is used to capture the PBS solution of mainstream smoke gas phase object water soluble ingredient is 7.4;
B. during the 3rd level of cigarette mainstream flue gas captures, select for use 70% glycerine to carry out the capture of mainstream smoke gas phase object organic phase composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101540973A CN101825528B (en) | 2010-04-23 | 2010-04-23 | Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101540973A CN101825528B (en) | 2010-04-23 | 2010-04-23 | Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101825528A CN101825528A (en) | 2010-09-08 |
| CN101825528B true CN101825528B (en) | 2011-08-24 |
Family
ID=42689571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101540973A Active CN101825528B (en) | 2010-04-23 | 2010-04-23 | Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101825528B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102156103B (en) * | 2011-03-08 | 2013-03-06 | 重庆烟草工业有限责任公司 | Determination method for indirectly measuring sulfureted hydrogen in mainstream smoke gas phase of cigarette by copper color method |
| CN105866351A (en) * | 2016-05-31 | 2016-08-17 | 安徽中烟工业有限责任公司 | Method for quickly measuring harmful ingredients in main stream smoke of cigarette |
| CN110988204B (en) * | 2019-11-07 | 2023-03-07 | 云南中烟工业有限责任公司 | Method for measuring migration volume of colorant smoke in filter tip by simulating cigarette smoking |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003041519A1 (en) * | 2001-11-09 | 2003-05-22 | Vector Tobacco Inc. | Extraction method for polyaromatic hydrocarbon analysis |
| US20040225450A1 (en) * | 2003-05-09 | 2004-11-11 | Ogden Michael W. | Analysis of substrates exposed to tobacco and components derived from tobacco |
| US20100186524A1 (en) * | 2008-02-05 | 2010-07-29 | Enertechnix, Inc | Aerosol Collection and Microdroplet Delivery for Analysis |
| CN101255456A (en) * | 2008-04-01 | 2008-09-03 | 中国烟草总公司郑州烟草研究院 | Toxicology neasuring evaluation method of cigarette smoke mutagenicity |
| CN101671733A (en) * | 2009-09-24 | 2010-03-17 | 中国烟草总公司郑州烟草研究院 | Improved cigarette smoke in-vitro contamination method |
-
2010
- 2010-04-23 CN CN2010101540973A patent/CN101825528B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101825528A (en) | 2010-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101825528B (en) | Three-stage trapping method of cigarette mainstream smoke for smoke toxicity detection | |
| CN102565233B (en) | Method for determining volatile and semi-volatile secondary metabolite in fresh tobacco leaves | |
| TWI304327B (en) | Method of treating tobacco extract to remove magnesium ions, method of preparing regenerated taobacco material, and regenerated tobacco material | |
| CN201555749U (en) | Device for trapping cigarette main flow smoke gas gas-phase objects | |
| CN101947435B (en) | Chitosan polycation additive for reducing phenols in cigarette smoke | |
| CN104270968A (en) | Liquid tobacco compositions | |
| CN202676676U (en) | System for detecting content of ammonia in mainstream smoke | |
| CN102809621B (en) | Method for detecting content of eugenol in cigarette mainstream smoke | |
| CN104237402A (en) | Extracting and measuring method of perfluorinated compounds in bark and leaves | |
| CN103323419A (en) | Method for testing nicotine alkaloid in tobacco and tobacco products | |
| CN104822282A (en) | Treatment of tobacco material | |
| CN102507846B (en) | Method for measuring release quantity of ammonia in tobacco leaves | |
| CN101285798A (en) | Test method for cigarette added tea polyphenol stability | |
| CN102221591B (en) | Method for evaluating adsorption effect of filter tip additive on phenolic compound in smoke | |
| CN106198809B (en) | A kind of flat-flavor flue-cured tobacco identification method based on activity threshold | |
| CN103163265A (en) | Method for determining residual quantity of myclobutanil by SPE-GC/ECD (Solid Phase Extraction-Gas Chromatography-Electron Capture Detector) | |
| CN106290611A (en) | A kind of Luzhou-flavor flue-cured tobacco authentication method based on activity threshold | |
| CN102499479A (en) | Complex adsorbent capable of reducing release amount of heavy metals in mainstream smoke of cigarettes, preparation method for complex adsorbent and application of complex adsorbent | |
| RU2291658C2 (en) | Cigarette filter | |
| CN104223357B (en) | Green Tea Extract tobacco mellowing agent and preparation thereof, application in beating and double roasting leaves moisting | |
| CN106290610B (en) | A kind of delicate fragrance type flue-cured tobacco identification method based on activity threshold | |
| CN105004819A (en) | Method for measuring content of HCN (hydrogen cyanide) in main stream smoke of cigarettes | |
| CN102692483A (en) | Method for separating and measuring taste components of main stream smoke particulate matter | |
| CN104479865A (en) | Preparation method of lotus leaf extract and application of lotus leaf extract in cigarettes | |
| CN105067390A (en) | Method for measuring ammonia content in mainstream smoke of cigarettes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |