CN101810886A - New application of carbonyl iron powder - Google Patents
New application of carbonyl iron powder Download PDFInfo
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- CN101810886A CN101810886A CN201010128060A CN201010128060A CN101810886A CN 101810886 A CN101810886 A CN 101810886A CN 201010128060 A CN201010128060 A CN 201010128060A CN 201010128060 A CN201010128060 A CN 201010128060A CN 101810886 A CN101810886 A CN 101810886A
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Abstract
The invention discloses a new application of carbonyl iron powder, in particular to the application of the carbonyl iron powder in the preparation of a magnetic medium for tumor magnetic thermotherapy. The diameter of the carbonyl iron powder can be 1-50 micro meters, and the preferred diameter is 7-10 micro meters. Compared with the prior art, the carbonyl iron powder is used for the arterial embolism magnetic thermotherapy medium, and can effectively prevent the curative effect from being influenced by the venous return of the medium, thereby ensuring high safety; the carbonyl iron powder has good biocompatibility, thus surface modification is not required; and temperature rise tests can show that the medium has good heating effect, thereby achieving good curative effect.
Description
Technical field
The present invention relates to tumor magnetic induction treatment and use the magnetic medium field, especially relate to a kind of purposes of carbonyl iron dust.
Background technology
Tumor is to cause one of human dead topmost disease, and the research of treatment tumor is emphasis and the focus that medical circle is paid close attention to most all the time.
Traditional tumor therapeuticing method is operation, radiation and chemotherapy.Infantile tumour can adopt operative treatment, the operation but common only 10%～20% tumour patient is had an opportunity, and perform the operation and bring bigger misery to patient easily, postoperative recurs easily and shifts; Tumor had belonged to middle and advanced stage when most patients was made a definite diagnosis, and radiation and chemotherapy also destroys normal cell when killing tumor cell, and toxic and side effects is bigger.
Medical engineering and medical physics technology rapid development have been quickened clinicalization of naturopathy progress, high-new physical therapeutic technic is brought into play enormous function in the clinical treatment of tumor, various naturopathy means such as ray, electricity, light, ultrasonic, radio frequency and microwave etc. are applied to the clinical therapy of tumor field more and more, particularly merge the use in conjunction of multidisciplinary advanced comprehensive treatment means (interventional therapy, chemotherapy, targeting thermotherapy, micro-nano technology etc.), thereby be expected to further improve clinical therapy of tumor effect and patient's life quality.
In recent years, the basic physical principle that the tumor magnetic induction thermotherapy technology that develops rapidly will " be responded under the ferromagnetic material alternating magnetic field and heat up " is applied to the clinical treatment of tumor.Brand-new treatment theory, the accurate targeting of treatment, excite the outstanding advantages such as height mixing together of the biomedical specificity base research of active immunity, make this treatment provide to have very that important theory is worth and application prospect, and be expected to again make a breakthrough on the clinical treatment of tumor.
The magnetic induction thermotherapy be a kind of internal target of organizing to thermotherapy, be expected to overcome many limitation that present thermotherapy technology exists.The magnetic induction thermotherapy comprises two steps, first-selection is that magnetic-particle is imported in the tumor tissues, then tumor locus is exposed in the alternating magnetic field that adds, the magnetic particle in the tumor tissues will be induced at alternating magnetic field and be given birth to heat down, plays the effect of internal target to thermotherapy of organizing.According to how ferromagnetic particle imported to the approach in the tumor tissues, the research of magnetic induction thermotherapy 4 branches of having derived are respectively plantation thermotherapies in thermotherapy and the tissue in arterial thrombosis thermotherapy, direct injection thermotherapy, the cell.Be used for the thermotherapy of clinical treatment carcinoma of prostate, nervous system neoplasms and other tumors except plantation thermotherapy in organizing at present, other several magnetic induction thermotherapy technology still are in the preclinical study stage.
In the arterial thrombosis thermotherapy, because being ferromagnetic material, the base therapy mechanism of magnetic induction thermotherapy under alternating magnetic field, responds to the physics characteristic that heats up, therefore selecting suitable thermal medium is the basic problem of magnetic induction thermotherapy.Except that satisfying excellent biological compatibility and magnetic hysteresis intensification requirement, the medium yardstick also is the key factor of image curative effect for magnetic induction thromboembolism thermotherapy medium.The research of the embolism materials of arterial thrombosis thermotherapy at present mainly concentrates on γ-Fe
2O
3And Fe
3O
4, particulate yardstick can be micron-sized magnetic microsphere or nano level magnetic liquid, some results of study show that the nanoscale medium can infiltrate venous return and affects the treatment.The present direct injection thermotherapy that adopts is with the nano-level iron oxysome or be aided with the macromolecular material coating, and the yardstick of above-mentioned material, safety and the efficiency of heating surface all can not satisfy magnetic induction thromboembolism thermotherapy requirements for clinical application.
Summary of the invention
The new purposes that the purpose of this invention is to provide carbonyl iron dust.
The new purposes of carbonyl iron dust provided by the present invention is the application of carbonyl iron dust in the magnetic medium (thermotherapy medium) that preparation magnetic induction thermotherapy is used.
Wherein, the diameter of described carbonyl iron dust can be 1～50 μ m, and preferred diameter is 1～10 μ m.
The magnetic medium that described magnetic induction thermotherapy is used specifically can be the magnetic medium that arterial thrombosis magnetic induction thermotherapy is used.
Described magnetic medium is the suspension of carbonyl iron dust and iodized oil.
The concentration of carbonyl iron dust can be 10～100mg/ml in the described suspension, and preferred concentration range for is 20～100mg/mL, most preferably is 60mg/mL.
Compared with prior art, carbonyl iron dust provided by the invention is used for arterial thrombosis magnetic induction thermotherapy medium, can effectively avoid medium to enter venous return and affect the treatment, safety is good, and carbonyl iron dust has excellent biological compatibility not to be needed it is carried out finishing, can prove that by elevated temperature test this medium of employing has good intensification effect, thereby good effect.
Description of drawings
Fig. 1 is the sketch map of the intensification situation of variable concentrations carbonyl iron dust-iodized oil suspension when magnetic field intensity is 110Gs among the embodiment 1.
The sketch map of maximum temperature when Fig. 2 is 110Gs for variable concentrations carbonyl iron dust-iodized oil suspension among the embodiment 1 in field supply intensity.
Fig. 3 is the sketch map of the dependency of carbonyl iron dust among the embodiment 1-iodized oil suspension intensification and dosage.
Fig. 4 is the sketch map of the intensification situation of 60mg/ml carbonyl iron dust-iodized oil suspension under different magnetic field intensity among the embodiment 2.
The specific embodiment
In order to make those skilled in the art better understand technical scheme of the present invention, the present invention is described in further detail below in conjunction with the drawings and specific embodiments.Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and material if no special instructions, all can obtain from commercial channels.
The suspension that carbonyl iron dust provided by the invention and iodized oil are made can be used for tumor magnetic induction thermotherapy, the particularly magnetizing mediums of arterial thrombosis thermotherapy.
The heat production effect test of carbonyl iron dust-iodized oil suspension under action of alternating magnetic field
The carbonyl iron dust of embodiment 1, variable concentrations-iodized oil suspension is in certain Determination on condition that heats up under the alternating magnetic field that adds
The preparation of carbonyl iron dust-iodized oil suspension:
Take by weighing 30,40,50,60, the 70mg carbonyl iron dust, particle size range is 1～10 μ m, is positioned over respectively in the 1ml centrifuge tube, respectively add the 1ml iodized oil in each centrifuge tube, ultrasonic mixing 5min, the extracting repeatedly of reuse 1ml micro sample adding appliance forms uniform suspension until carbonyl iron dust and iodized oil.
The concrete influence of measuring concentration to carbonyl iron dust-iodized oil suspension intensification situation:
Variable concentrations carbonyl iron dust-iodized oil suspension is placed the constant temperature jacket of small-sized magnetic induction experimental provision, with two thermoelectricity on a rare occasion root insert in the centrifuge tube, detect the suspension temperature; Another root inserts in the constant temperature jacket, the testing environment temperature.It is 110Gs that field supply intensity is set, and the temperature that controls environment writes down the intensification situation of variable concentrations suspension about 37 ℃.
The intensification situation shows the carbonyl iron dust of variable concentrations-iodized oil suspension under the alternating magnetic field in certain adding, when field supply intensity is 110Gs, carbonyl iron dust-iodized oil suspension is along with the rising of concentration, the obvious more (see figure 1) that heats up, following high more (see figure 2) of the maximum temperature that can reach in magnetic field.
Make rectilinear regression and correlation analysis by the temperature that suspension reached and its corresponding concentration to each concentration of 3min, 10min, can draw: when carbonyl iron dust reached maximum temperature in the temperature-rise period neutralization, suspension concentration became positive straight line dependency relation (p with its corresponding temperature
3min=0.0059; p
10min=0.0040), and the better (r of linear relationship
3 Min=0.943; r
10min=0.956) (see figure 3).
Embodiment 2, the carbonyl iron dust-iodized oil suspension Determination on condition that under difference adds alternating magnetic field, heats up
With concentration be carbonyl iron dust-iodized oil suspension of 60mg/ml according to the experimental technique among the embodiment 1, detect respectively that suspension is 50,80 in field supply intensity, the intensification situation under the 110Gs.
Conclusion: certain density carbonyl iron dust-iodized oil suspension Determination on condition that under difference adds alternating magnetic field, heats up: carbonyl iron dust-when iodized oil suspension concentration is 60mg/ml, increase along with magnetic field intensity, suspension heats up more obvious, and under the current intensity of 110Gs, the maximum temperature that suspension can reach is apparently higher than the maximum temperature (see figure 4) under 80Gs and 50Gs current intensity.
The micron order magnetic particle is under alternating magnetic field, by hysteresis or lax mechanism (rearrangement of magnetic vector in the magnetic field) heat production of Neel.The magnetic particle of unit mass identical component (inferior magnetic domain particle) is more than 1000 times of multidomain particles in the heat production rate under the identical alternating magnetic field, therefore, can judge that micron order magnetic granule is a kind of more satisfactory thermal medium.
Carbonyl iron dust-iodized oil suspension is placed under the alternating magnetic field by variable concentrations, and when field supply immobilized, along with the rising of suspension concentration, temperature rose fast more, and temperature is high more in the time of steadily, and the ambient temperature of monitoring does not change.
By each concentration suspension is returned and correlation analysis as the straight line line linearity of going forward side by side in the temperature of rising stage (3min) and plateau (10min), carbonyl iron dust is when the temperature-rise period neutralization reaches maximum temperature as can be seen, suspension concentration and elevated temperature are in linear relation, and are better linearity dependency relation (see figure 3).This points out us to realize the control of heating by control suspension concentration.
And certain density carbonyl iron dust-iodized oil suspension is placed under the alternating magnetic field, along with the rising of magnetic field intensity, it is fast more that temperature rises, and temperature is high more steadily the time, and the ambient temperature of monitoring does not change.
Can draw thus, the heat production under action of alternating magnetic field of the carbonyl iron dust that present embodiment is selected for use-iodized oil suspension is effective, and heating property is stable.And in suspension concentration is 60mg/ml, when magnetic field intensity is 110Gs, can reach ideal oncotherapy temperature (more than 45 ℃).
The experiment grouping:
A. negative control group (RPIM-1640 culture medium);
B.10mg/ml-100% lixiviating solution group (10mg/ml carbonyl iron dust, 100% lixiviating solution);
C.10mg/ml-50% lixiviating solution group (10mg/ml carbonyl iron dust, 50% lixiviating solution);
D. positive controls (64g/L phenol solution)
Experimental technique:
The preparation of carbonyl iron dust lixiviating solution: (medical apparatus and instruments biological assessment standard the 5th part: the cell toxicity test in vitro method) prescribed material surface area and lixiviate medium volume ratio should be (0.5-6) cm according to standard GB/T16886.521997
2/ ml.Carbonyl iron dust (particle size range is 1～10 μ m) with the lixiviate of RPIM-1640 culture fluid, is placed 37 ℃ of incubator lixiviate 24h.
Cell culture and mtt assay detect: (available from Beijing consonance cell bank, catalog number (Cat.No.) L929) carries out In vitro culture with the L-929 cell, and reach growing state through going down to posterity stable.Take the logarithm this cell of trophophase is made cell suspension after 0.25% trypsinization, with 1 * 10
4The cell concentration of individual/ml is inoculated in 96 well culture plates, and (200 μ l/ holes, n=8), every hole inoculates 2 * 10
3Individual cell places 5%CO
2, cultivate 48h in 37 ℃ of incubators.Observation of cell is adherent good, discards original fluid, and every culture plate is pressed negative control, different group experimental grouies, and positive control, liquid, every group 8 hole are changed in the grouping of blank partition.Routine is positioned over 5%CO
2, cultivate in 37 ℃ of incubators.
The cell of cultivating 2 days, 4 days, 7 days (changing liquid once in per 2 days) is carried out mtt assay to be detected.In 96 orifice plate cell culture holes, add 5mg/ml MTT (20 μ l/ hole) back and continue to cultivate 4h, discard stock solution and add DMSO 150 μ l/ holes, concussion culture plate 15min treats to use enzyme-linked immunosorbent assay instrument to measure the absorbance (OD value) in each hole at the 490nm wavelength after the bluish violet crystallization stripping.The cell toxicant level is according to State Standard of the People's Republic of China GB/T16886.10-20050 " medical apparatus and instruments biological assessment the 5th part: vitro cytotoxicity test " classification.Experimental result sees Table 1-3.
OD value, cell proliferation degree and the cell toxicant level of each group cultivation 2d of table 1 (x ± s, n=8)
| Group | Absorbance (OD) | Cell proliferation degree RGR (%) | The cell toxicant level |
| 10mg/ml-100% lixiviating solution group | ??0.773±0.097 | ?81.88 | ??1 |
| 10mg/ml-50% lixiviating solution group | ??0.952±0.176 | ?100.8 | ??0 |
| Negative control group | ??0.943±0.176 | ?100 | ??0 |
| Positive controls | ??0 | ?0 | ??5 |
OD value, cell proliferation degree and the cell toxicant level of each group cultivation 4d of table 2 (x ± s, n=8)
| Group | Absorbance (OD) | Cell proliferation degree RGR (%) | The cell toxicant level |
| 10mg/ml-100% lixiviating solution group | ??1.562±0.213 | ?91.62 | ??1 |
| 10mg/ml-50% lixiviating solution group | ??1.659±0.266 | ?97.3 | ??1 |
| Negative control group | ??1.705±0.306 | ?100 | ??0 |
| Positive controls | ??0 | ?0 | ??5 |
OD value, cell proliferation degree and the cell toxicant level of each group cultivation 7d of table 3 (x ± s, n=8)
| Group | Absorbance (OD) | Cell proliferation degree RGR (%) | The cell toxicant level |
| 10mg/ml-100% lixiviating solution group | ??2.212±0.508 | ?90.25 | ??1 |
| Group | Absorbance (OD) | Cell proliferation degree RGR (%) | The cell toxicant level |
| 10mg/ml-50% lixiviating solution group | ??2.510±0.506 | ?102.44 | ??0 |
| Negative control group | ??2.450±0.306 | ?100 | ??0 |
| Positive controls | ??0 | ?0 | ??5 |
By table 1,2,3 as can be seen, and at the 2nd, 4,7 day of each group cultivation, 100% lixiviating solution group cultured cell poison level was 1,50% lixiviating solution group cultured cell poison level and is respectively 0 or 1, compares equal no significant difference with negative control group.It can be said that the basic no cytotoxicity effect of carbonyl iron dust that bright test detected.
Present embodiment has carried out Cytotoxic evaluation (table 1,2,3) respectively to the carbonyl iron dust lixiviating solution of variable concentrations.Carbonyl iron dust lixiviating solution with variable concentrations is cultivated l cell L-929, at the 2nd, 4,7 day, 100% lixiviating solution group cultured cell poison level is respectively 1, compares equal no significant difference with negative control group, and 50% lixiviating solution group cultured cell poison level is respectively 0 or 1.It can be said that the basic no cytotoxicity effect of carbonyl iron dust that bright test detected, have the good cell compatibility, meet the basic demand that organism is used, confirmed that carbonyl iron dust has the bio-safety basis that human body is used.
Male Mus (about the body weight 200g) 15 of experimental subject: Wistar is divided into two groups at random.One group is PBS buffer injection group (matched group), 6; Another group is carbonyl iron powder (particle size range is 1～10 μ m)-PBS suspension injection group (experimental group), 9.
Experimental technique: autoclaved carbonyl iron dust and PBS buffer are prepared in the ratio of 30mg/ml.Experimental group is injected once, and injection volume is 2.5ml/kg, the PBS buffer of matched group injection same amount.Two groups are all adopted tail vein injection.
Blood sampling: injection back the 3rd day, all rats are anaesthetized with absolute ether, and eyeball is got blood, and every of matched group and experimental group are got 2ml blood and are not added anticoagulant, and 3000 commentaries on classics/min get 1ml serum and do the blood parameters test behind the centrifugal 10min; Other gets 2 bleed anticoagulant test leukocyte, erythrocyte number and content of hemoglobin.
Pathological examination: after getting blood, put to death rat, get liver, nephridial tissue, 10% formaldehyde fixed, row pathological examination.
By the result of table 4 experimental group and matched group blood parameters more as can be seen: rat behind tail vein injection, the glutamate pyruvate transaminase of experimental group (ALT), glutamic oxaloacetic transaminase, GOT (AST), creatinine (CREA), carbamide (UREA), uric acid (UA) content and matched group no difference of science of statistics (p＞0.05).This shows carbonyl iron dust to not causing the change of blood parameters value after the rat tail vein injection, and the hepatic and renal function of blood rat is not had obvious influence.
The result of table 4 experimental group and matched group blood parameters relatively
| Grouping | ??ALT??(U/L) | ??AST??(U/L) | ??CREA??(umol) | ??UREA??(mmol/L) | ??UA??(umol/L) |
| Experimental group 1 | ??93 | ??208 | ??37 | ??7.31 | ??32 |
| Experimental group 2 | ??79 | ??199 | ??32 | ??6.68 | ??62 |
| |
??71 | ??173 | ??31 | ??5.39 | ??74 |
| Grouping | ??ALT??(U/L) | ??AST??(U/L) | ??CREA??(umol) | ??UREA??(mmol/L) | ??UA??(umol/L) |
| Experimental group 4 | ??89 | ??177 | ??35 | ??6.56 | ??77 |
| Experimental group 5 | ??90 | ??210 | ??29 | ??7.02 | ??58 |
| Experimental group 6 | ??78 | ??224 | ??26 | ??6.17 | ??51 |
| Experimental group 7 | ??82 | ??218 | ??25 | ??5.99 | ??69 |
| Experimental group 8 | ??70 | ??227 | ??28 | ??6.33 | ??73 |
| Experimental group 9 | ??77 | ??230 | ??32 | ??6.26 | ??65 |
| The experimental group average | ??81±8.21 | ??207.33±20.82 | ??30.56±3.97 | ??6.41±0.57 | ??62.33±14.07 |
| Matched group 1 | ??77 | ??254 | ??26 | ??7.74 | ??74 |
| Matched group 2 | ??86 | ??220 | ??33 | ??6.25 | ??79 |
| Matched group 3 | ??92 | ??226 | ??28 | ??6.89 | ??62 |
| Matched group 4 | ??106 | ??221 | ??29 | ??5.98 | ??80 |
| Matched group 5 | ??73 | ??192 | ??37 | ??6.93 | ??59 |
| Matched group 6 | ??74 | ??181 | ??30 | ??6.17 | ??53 |
| The matched group average | ??84.67±12.80 | ??215.67±26.02 | ??30.5±3.94 | ??6.66±0.66 | ??67.83±11.34 |
| Average is (p) relatively | ??0.5082 | ??0.5032 | ??0.9792 | ??0.4503 | ??0.4396 |
The result of table 5 experimental group and matched group leukocyte, erythrocyte number and content of hemoglobin relatively
| Grouping | ??WBC(10 9/L) | ??RBC(10 12/L) | ??HGB(g/L) |
| Experimental group 1 | ??3.8 | ??5.42 | ??119 |
| Experimental group 2 | ??11.2 | ??4.95 | ??110 |
| |
??6.7 | ??5.65 | ??123 |
| |
??10.2 | ??5.27 | ??112 |
| Experimental group 5 | ??9.8 | ??6.25 | ??121 |
| Experimental group 6 | ??8.9 | ??6.45 | ??131 |
| Grouping | ??WBC(10 9/L) | ??RBC(10 12/L) | ??HGB(g/L) |
| Experimental group 7 | ??13.9 | ??6.33 | ??121 |
| |
??9.7 | ??5.96 | ??117 |
| |
??12.3 | ??5.13 | ??106 |
| The experimental group average | ??10.72±2.34 | ??5.72±0.56 | ??117.78±7.56 |
| Matched group 1 | ??3.6 | ??5.10 | ??115 |
| Matched group 2 | ??6.4 | ??5.41 | ??112 |
| Matched |
??8.8 | ??5.27 | ??119 |
| Matched |
??7.2 | ??4.69 | ??104 |
| Matched group 5 | ??18.2 | ??5.76 | ??118 |
| Matched group 6 | ??9.6 | ??5.92 | ??121 |
| The matched group average | ??8.97±4.98 | ??5.36±0.45 | ??114.83±6.18 |
| Average is (p) relatively | ??0.3712 | ??0.2170 | ??0.4431 |
By the result of table 5 experimental group and matched group leukocyte, erythrocyte number and content of hemoglobin more as can be seen: rat behind tail vein injection, the wherein leukocyte count of experimental group, RBC number and content of hemoglobin and matched group no difference of science of statistics (p＞0.05).This shows that carbonyl iron dust does not change rat tail vein injection back leukocyte count, RBC number and content of hemoglobin, does not cause immunoreation.
The present embodiment result shows, the PBS suspension of tail vein injection carbonyl iron dust does not cause the changes of biochemical indexes (table 4) of rats'liver, renal function, do not cause the change (table 5) of rat RBC number, leukocyte count and content of hemoglobin, the histological examination result shows that obviously damage and inflammatory reaction do not appear in rats'liver, the nephridial tissue behind tail vein injection carbonyl iron dust-PBS suspension 3d.This shows that carbonyl iron dust does not cause immunoreation after injecting blood, liver, renal function is not impacted yet, and has good biocompatibility.Therefore, when carrying out arterial thrombosis, can not cause the variation of hematological indices with carbonyl iron dust, and liver, nephrotoxicity.
Embodiment 5,
Hepatic artery is selected thromboembolism: new zealand white rabbit 2-3kg (10, female) is divided into two groups at random: A group experimental group (5) is inserted tube injection carbonyl iron dust-iodized oil suspension; B group matched group (5) is only inserted not injection suspension of conduit.
Art fasting in preceding 24 hours.The experimental group animal is adopted 2% pentobarbital sodium auricular vein injection general anesthesia, rabbit lain on the back be fixed in operating-table, the depilation of left side inguinal region, sterilization, drape, cut skin, passivity is isolated femoral artery and is about 2cm, and near, far-end is respectively worn 1 on No. 4 lines, mentions after the far-end ligation, the 18G plastic bushing is needled into femoral artery, release nook closing member and see the ejection of cerise blood, gently carry the near-end silk thread and can reduce hemorrhagely, insert microtubular and seal wire and take over control the valve that backflows along the plastic sheath bobbin.Selectivity inserts coeliac artery with conduit, after radiography confirms, the super selection of conduit is inserted into proper hepatic artery, slowly injects carbonyl iron dust (particle size range is 1～10 μ m)-iodized oil suspension 0.8-1.0ml of 60mg/ml, after injection finishes, withdraw from conduit, the femoral artery ligation, local stitching, intramuscular injection benzylpenicillin 400,000 units, 3 days, close cage and raise.Can inject lignocaine by conduit in the tremulous pulse intervention procedure and suppress vasospasm.
Thermotherapy: arterial thrombosis is after 8 hours, with the experimental group animal with 2% pentobarbital sodium auricular vein injecting anesthetic, get the epigastrium median incision and cut the abdominal cavity, fully expose liver, insert the thermocouple monitoring variations in temperature at hepatic tissue ishemic part and rectum position respectively.Animal is placed under the small-sized magnetic induction experimental provision, and it is 110Gs that magnetic field intensity is set, and 300kHz is about heating 20min.
Histopathologic examination: thermotherapy is all put to death the experimental group rabbit after finishing, and matched group is randomly drawed 2 rabbits and put to death, and gets normal liver tissue solution soaking in 10% formaldehyde immediately, paraffin embedding, section, HE dyeing and prussian blue staining, row histopathologic examination.
Tremulous pulse interventional embolization result:
Celiac arteriogram shows that it has two big branches: one is the arteria gastrolienalis of lower-left traveling, and pumping blood is to the harmonization of the stomach spleen; Another is the arteria gastroherpatica to upper right traveling, pumping blood is to stomach, liver, pancreas and a part of duodenum, this tremulous pulse is punished out three branches in left, center, right at about 0.5cm-1.0cm, wherein, right medial branch (5/8) or the middle lateral branch (3/8) that props up are the feeding artery of liver and gallbladder, i.e. proper hepatic artery.Proper hepatic artery is divided into left branch of proper hepatic artery and ramus dexter arteriae hepaticae propriae, and cystic artery is from ramus dexter arteriae hepaticae propriae, and the left branch of proper hepatic artery majority is the direct continuity of proper hepatic artery.
Add the alternating magnetic field thermotherapeutic result:
Heat after 20 minutes, 0.111 ± 0.050 ℃/s of experimental group hepatic tissue programming rate average out to, average 0.001 ± 0.001 ℃/s of rectum programming rate, each point for measuring temperature temperature rising number of degrees sees Table 6.Edge hepatic tissue temperature heats up when the heating beginning immediately, about 5 minutes Shi Ke rise to more than 42 ℃ in heating, when being heated to 20 minutes, experimental group animal liver tissue temperature is arranged even rising to more than 50 ℃, but it is less that each animal rectal temperature changes, and whole heating process all is no more than 28 ℃.Obvious intensification does not appear in matched group.
Table 6 adds the temperature that experimental group Hepar Leporis seu Oryctolagi tissue and rectum record at each time point under the alternating magnetic field
| Time (sec) | ??0 | ??60 | ??120 | ??300 | ??600 | ??1200 |
| Hepatic tissue 1 | ??27 | ??34 | ??40 | ??42 | ??43 | ??43 |
| Hepatic tissue 2 | ??28 | ??42 | ??45 | ??45 | ??46 | ??50 |
| |
??26 | ??46.6 | ??46.6 | ??47.5 | ??48.1 | ??48.6 |
| |
??27 | ??30.5 | ??51.3 | ??52.5 | ??52.3 | ??52.5 |
| Hepatic tissue 5 | ??28 | ??36 | ??43 | ??43.3 | ??44.2 | ??45.1 |
| Rectum 1 | ??28 | ??27 | ??27 | ??28 | ??28 | ??28 |
| Rectum 2 | ??27 | ??28 | ??28 | ??27 | ??27 | ??27 |
| Time (sec) | ??0 | ??60 | ??120 | ??300 | ??600 | ??1200 |
| |
??25 | ??26 | ??27 | ??27 | ??26 | ??27 |
| |
??26 | ??26 | ??27 | ??26 | ??27 | ??26 |
| Rectum 5 | ??26 | ??26 | ??27 | ??27 | ??26 | ??26 |
The pathological examination result:
After the HE dyeing, as seen downright bad in a organized way around the carbonyl iron dust particle deposition.
Prussian blue staining is observed, and visible carbonyl iron dust is assembled in Hepatic artery blood conduit, and has the part iron powder to enter sinus hepaticus, liver rope, Disse chamber.Also have carbonyl iron dust to enter hepatocyte, even enter liver cell nuclear.And matched group does not see that iron powder distributes.
The foregoing description shows that it has good heat production performance in adding alternating magnetic field, and behind the trans-hepatic artery thromboembolism, body is interior through inducing heating also can make hepatic tissue reach oncotherapy temperature (more than 45 ℃) in 20 minutes.The present embodiment temperature-measuring results shows that the average in vivo per minute of carbonyl iron dust-iodized oil suspension rises about 6 ℃ approximately, and the intensification effect is obvious.
On vertical as can be known, carbonyl iron dust and carbonyl iron dust and iodized oil suspension can be applied to the magnetic medium in the tumor arterial thrombosis magnetic induction thermotherapy, adopting the diameter of carbonyl iron dust is 1～10 μ m, concentration is that carbonyl iron dust-iodized oil suspension of 60mg/ml is used for arterial thrombosis magnetic induction thermotherapy medium and can effectively avoids medium to enter venous return affecting the treatment, safety is good, and carbonyl iron dust has excellent biological compatibility not to be needed it is carried out finishing, can prove that by elevated temperature test this medium of employing has good intensification effect, thereby good effect.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. the application of carbonyl iron dust in the magnetic medium that preparation magnetic induction thermotherapy is used.
2. application according to claim 1 is characterized in that: the diameter of described carbonyl iron dust is 1～50 μ m.
3. application according to claim 2 is characterized in that: the diameter of described carbonyl iron dust is 1～10 μ m.
4. according to arbitrary described application among the claim 1-3, it is characterized in that: the magnetic medium that described magnetic induction thermotherapy is used is the magnetic medium that arterial thrombosis magnetic induction thermotherapy is used.
5. application according to claim 4 is characterized in that: the magnetic medium that described arterial thrombosis magnetic induction thermotherapy is used is the suspension of carbonyl iron dust and iodized oil.
6. application according to claim 5 is characterized in that: the concentration of carbonyl iron dust is 10～100mg/ml in the described suspension.
7. application according to claim 6 is characterized in that: the concentration of carbonyl iron dust is 20～100mg/ml in the described suspension.
8. application according to claim 7 is characterized in that: the concentration of carbonyl iron dust is 60mg/ml in the described suspension.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108525128A (en) * | 2018-03-26 | 2018-09-14 | 清华大学 | Application of the liquid metal as tumour magnetic thermotherapy medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1849726A (en) * | 2003-09-09 | 2006-10-18 | 莱尔德技术股份有限公司 | Microwave-absorbing form-in-place paste |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1849726A (en) * | 2003-09-09 | 2006-10-18 | 莱尔德技术股份有限公司 | Microwave-absorbing form-in-place paste |
Non-Patent Citations (2)
| Title |
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| 江薇等: "动脉栓塞热疗用羰基铁粉磁热效应和细胞毒性研究", 《中国微创外科杂志》 * |
| 罗永弟: "超细羰基铁发展、市场及应用前景", 《四川冶金》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108525128A (en) * | 2018-03-26 | 2018-09-14 | 清华大学 | Application of the liquid metal as tumour magnetic thermotherapy medium |
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Application publication date: 20100825 |