CN101810881A - Preparation method of sericin/hydroxyapatite composite porous bracket - Google Patents
Preparation method of sericin/hydroxyapatite composite porous bracket Download PDFInfo
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- CN101810881A CN101810881A CN201010157889A CN201010157889A CN101810881A CN 101810881 A CN101810881 A CN 101810881A CN 201010157889 A CN201010157889 A CN 201010157889A CN 201010157889 A CN201010157889 A CN 201010157889A CN 101810881 A CN101810881 A CN 101810881A
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Abstract
The invention discloses a preparation method of a sericin/hydroxyapatite composite porous bracket. The method comprises the following steps: respectively synthesizing hydroxyapatite powder and preparing a sericin solution, mixing according to different mass proportions, and using the freeze-drying method to prepare a sericin/hydroxyapatite composite porous bracket material. The prepared sericin/hydroxyapatite composite porous bracket material has the advantages of simple preparation process, short preparation time, easily-satisfied preparation conditions, no porogenic agent addition, less and cheap reagent and low cost. Besides, the prepared bracket material has higher porosity and mechanical property, and the pores are uniformly dispersed and are communicated mutually.
Description
Technical field
The present invention relates to the processing and utilization field of sericin, sericin/hydroxyapatite composite porous bracket preparation methods in particularly a kind of bone tissue engineer.
Background technology
Bone injury and bone are damaged to be disease very common in the daily life.Traditional medicine mainly adopts from the method for body and heteroplastic transplantation carries out the damaged reparation of bone, but this method occurs drawbacks such as new damage and the reflection of generation immunologic rejection, is not rational bone restorative procedure.And bone tissue engineer is with a spot of histiocyte repairing bone defect behind cultured and amplified in vitro, can reach perfect form reparation according to any plastotype of the damaged situation of bone simultaneously, has opened up new approach for realizing the not damaged reparation.
Sericin (Silk Sericin is called for short SS) is a kind of globular preteins, and relative molecular weight is 1.4～31.4 ten thousand, is made up of 18 seed amino acids.Sericin has good water-solubility, water absorption, biocompatibility, and the activity of energy restraint of tyrosinase has characteristics such as non-oxidizability, anticoagulation, antitumor.As a kind of native protein resource, sericin can be applicable to biomaterial and field of medicaments etc.
(hydroxyapatite, HA Fig. 1) are a kind of bioactive ceramics to hydroxyapatite, and its chemical composition and crystalline texture are similar to the apatite in the skeleton.Exchange reaction can take place with the aminoacid that contains carboxyl, protein, organic acid etc. in the Ca2+ in the HA molecule, have good bone conduction performance and biological activity, can form firm synostosis with osseous tissue and promote bone growth, phase is more stable, avirulence, inflammatory are that the well behaved bone of generally acknowledging is repaired substitution material.Fragility is big, toughness is relatively poor, mechanical property is not enough but also exist, and degraded slowly and easily from shortcomings such as implantation site migrations, this has all limited its use as human material implantation body.
Summary of the invention
The objective of the invention is synthesizing hydroxylapatite powder and preparation sericin solution respectively, both are pressed the different quality mixed, prepare the sericin/hydroxyapatite composite porous bracket material by freeze-drying method.
The preparation method of a kind of sericin/hydroxyapatite composite porous bracket of the present invention may further comprise the steps:
(1) measures a certain amount of sericin solution and place beaker,, keep sericin consistent with the ratio of total amount in solution of hydroxyapatite in proportion to wherein adding hydroxyapatite powder;
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min;
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs;
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately;
(5) freezing 12h rear plate sample is taken out, under 20 ℃ of conditions of room temperature, rise again and handle 4h;
(6) flat samples after will rising again is put into-20 ℃ of refrigerator and cooled once more and is frozen, and puts into-80 ℃ of freezer dryer lyophilizations then, makes the sericin/hydroxyapatite composite porous bracket of different proportion.
Described sericin solution, its preparation method may further comprise the steps:
(1) will remove Bombyxmori Linnaeus cocoon layer behind the pupa, put into the boiling water processing of coming unstuck;
(2) behind the 30min, stop steaming and decocting, filter roguing;
(3) the sericin solution after will filtering is put into Rotary Evaporators and is concentrated, and obtains the sericin solution of purpose concentration.
Described hydroxylapatite powder, its preparation method may further comprise the steps:
(1) gets saturated Ca (OH)
2Solution places 60 ℃ of water-baths, drips the H of 10%wt
3PO
4Solution, and Ca (OH)
2Solution and H
3PO
4The volume ratio of solution is 70: 1～2, during constantly fast stir and keep water temperature;
(2) with the method for dropping ammonia, the pH that adjusts solution remains on 8.7;
(3) drip H
3PO
4Continue to stir 3h behind the solution, stirring finishes to be placed on room temperature and leaves standstill 48h;
(4) the bottom precipitation is collected in the supernatant of inclining; Precipitation is cleaned 2-3 time with dehydrated alcohol earlier, and the reuse distilled water cleans 3-4 time;
(5) precipitation that will obtain is put into refrigerator and cooled and is frozen, and moves in the freeze dryer dryly then, grinds, and obtains hydroxyapatite powder.
The weight ratio of sericin and hydroxyapatite is 10: 1～1: 1 in a kind of preparation method of sericin/hydroxyapatite composite porous bracket, described step (1).
The preparation method of sericin solution, the consumption of water is to the cocoon layer submergence in the described step (1).
The preparation method of sericin solution, also comprise the sericin solution that obtains purpose concentration after, extract sericin solution, carry out sds gel electrophoresis, measure the sericin molecular weight.
The preparation method of hydroxylapatite powder, wherein step (1) is to get the saturated Ca of 700ml (OH)
2Solution places 60 ℃ of water-baths, drips the H of 10%wt
3PO
4Solution 11ml, during use magnetic stirring apparatus constantly to stir fast and keep water temperature.
Usefulness of the present invention is:
Sericin and hydroxyapatite are made the bone tissue engineer compound support frame material, and sericin can be used as the binding agent of hydroxyapatite particle on the one hand, suppresses the hydroxyapatite particle in the disengaging and the migration of implanting point; The mechanical property of material be can improve on the other hand, the toughness of polymer and the intensity and the hardness of inorganic particulate obtained.
The present invention adopts blending method to prepare the sericin/hydroxyapatite composite porous bracket material, and comprehensive both premium properties obtain a kind of biological activity bone renovating material of function admirable.
The molecular weight ranges of the sericin that the present invention is used is 97.2～200.0KDa, and the sericin/hydroxyapatite composite porous bracket material preparation process of preparation is simple, takes weak point, and preparation condition satisfies easily; Do not add porogen, agents useful for same is few and cheap, and cost is low; The prepared timbering material porosity of the present invention is higher, hole be uniformly dispersed and Kong Yukong between connect mutually; Has certain mechanical property.
Description of drawings
Fig. 1 is the pore structure photo of hydroxyapatite.
Fig. 2 is the pore structure photo of 9: 1 sericin/hydroxyapatite composite porous bracket material for the concentration ratio of inventive embodiments.
Fig. 3 is the pore structure photo of 7: 3 sericin/hydroxyapatite composite porous bracket material for the concentration ratio of inventive embodiments.
Fig. 4 is the pore structure photo of 5: 5 sericin/hydroxyapatite composite porous bracket material for the concentration ratio of inventive embodiments.
The specific embodiment
Below in conjunction with embodiment technical scheme of the present invention is described:
Embodiment 1: the preparation method of sericin solution may further comprise the steps:
(1) will remove Bombyxmori Linnaeus cocoon layer behind the pupa, put into the boiling water processing of coming unstuck; The consumption of water is being advisable the cocoon layer submergence;
(2) behind the 30min, stop steaming and decocting, filter roguing;
(3) the sericin solution after will filtering is put into Rotary Evaporators and is concentrated, and obtains the sericin solution of purpose concentration;
(4) extract sericin solution, carry out sds gel electrophoresis, measure the sericin molecular weight, the molecular weight ranges of sericin is 97.2～200.0KDa.
The preparation method of embodiment 2, hydroxylapatite powder may further comprise the steps:
(1) gets the saturated Ca of 700ml (OH)
2Solution places 60 ℃ of water-baths, drips the H of 10%wt
3PO
4Solution 11ml, during use magnetic stirring apparatus constantly to stir fast and keep water temperature;
(2) adopt the digital display pH meter to monitor the pH of solution, with the method for dropping ammonia, the pH that adjusts solution remains on 8.7;
(3) drip H
3PO
4Continue behind the solution with the 3h of rotating speed stirring faster, stirring finishes to be placed on room temperature and leaves standstill 48h;
(4) the bottom precipitation is collected in the supernatant of inclining; Precipitation is cleaned 2-3 time with dehydrated alcohol earlier, and the reuse distilled water cleans 3-4 time;
(5) precipitation that will obtain is put into refrigerator and cooled and is frozen, and moves in the freeze dryer dryly then, grinds, and obtains hydroxyapatite powder.
The preparation method of embodiment 3, a kind of sericin/hydroxyapatite composite porous bracket may further comprise the steps:
(1) measures a certain amount of sericin solution and place beaker,, keep sericin consistent with the ratio of total amount in solution of hydroxyapatite in proportion to wherein adding hydroxyapatite powder;
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min;
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs;
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately;
(5) freezing 12h rear plate sample is taken out, under 20 ℃ of conditions of room temperature, rise again and handle 4h;
(6) flat samples after will rising again is put into-20 ℃ of refrigerator and cooled once more and is frozen, and puts into-80 ℃ of freezer dryer lyophilizations then, makes the sericin/hydroxyapatite composite porous bracket of different proportion.
The Mechanics Performance Testing of embodiment 4, sericin/hydroxyapatite composite material
Adopt universal testing machine (AGS-J type, SHIMADZU company, Japan) that each sample is carried out Determination of Modulus.Diameter 5mm is made in each timbering material unification, and high 5mm's is cylindric, the support upper and lower surface is parallel to each other and smooth as far as possible.Ambient temperature and humidity are consistent.Every group of sample got three parallel samples, averages.
The preparation method of embodiment 5, a kind of sericin/hydroxyapatite composite porous bracket may further comprise the steps:
(1) accurately measures a certain amount of sericin solution and place beaker, in proportion to wherein adding hydroxyapatite powder.Make HA: the ratio of SS is 1: 9.
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min.
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs.
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately.
(5) freezing 12h rear plate is taken out, at ambient temperature, rise again and handle 4h, the sample of molding is taken out carefully.
(6) sample is put into once more-20 ℃ of refrigerator and cooled and frozen, put into-80 ℃ of freezer dryer lyophilizations then, finally make sericin/hydroxyapatite composite porous bracket HA10.Its density is 33mg/ml, and porosity is 50.1%, and elastic modelling quantity is 7.41MPa, Fig. 2.
The preparation method of embodiment 6, a kind of sericin/hydroxyapatite composite porous bracket may further comprise the steps:
(1) accurately measures a certain amount of sericin solution and place beaker, in proportion to wherein adding hydroxyapatite powder.Make HA: the ratio of SS is 3: 7.
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min.
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs.
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately.
(5) freezing 12h rear plate is taken out, at ambient temperature, rise again and handle 4h, the sample of molding is taken out carefully.
(6) sample is put into once more-20 ℃ of refrigerator and cooled and frozen, put into-80 ℃ of freezer dryer lyophilizations then, finally make sericin/hydroxyapatite composite porous bracket HA30.Its density is 39.7mg/ml, and porosity is 51.3%, and elastic modelling quantity is 10.56MPa, Fig. 3.
The preparation method of embodiment 7, a kind of sericin/hydroxyapatite composite porous bracket may further comprise the steps:
(1) accurately measures a certain amount of sericin solution and place beaker, in proportion to wherein adding hydroxyapatite powder.Make HA: the ratio of SS is 5: 5.
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min.
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs.
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately.
(5) freezing 12h rear plate is taken out, at ambient temperature, rise again and handle 4h, the sample of molding is taken out carefully.
(6) sample is put into once more-20 ℃ of refrigerator and cooled and frozen, put into-80 ℃ of freezer dryer lyophilizations then, finally make sericin/hydroxyapatite composite porous bracket HA50.Its density is 52.7mg/ml, and porosity is 33%, and elastic modelling quantity is 15.64MPa, Fig. 4.
At last, it is also to be noted that what more than enumerate only is specific embodiments of the invention.Obviously, the invention is not restricted to above examples of implementation, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (7)
1. the preparation method of a sericin/hydroxyapatite composite porous bracket is characterized in that may further comprise the steps:
(1) measures a certain amount of sericin solution and place beaker,, keep sericin consistent with the ratio of total amount in solution of hydroxyapatite in proportion to wherein adding hydroxyapatite powder;
(2) mixed solution of sericin and hydroxyapatite is put into ultrasonic cleaner, oscillation treatment 3～5min;
(3) taking out mixed solution, is 0.5% glutaraldehyde cross-linking agent to the concentration that wherein slowly drips trace, and magnetic stirring apparatus slowly stirs;
(4) mixed solution is moved into 96 porocyte culture plates fast, and put into-20 ℃ of refrigerator precooling 12h immediately;
(5) freezing 12h rear plate sample is taken out, under 20 ℃ of conditions of room temperature, rise again and handle 4h;
(6) flat samples after will rising again is put into-20 ℃ of refrigerator and cooled once more and is frozen, and puts into-80 ℃ of freezer dryer lyophilizations then, makes the sericin/hydroxyapatite composite porous bracket of different proportion.
2. preparation method according to claim 1 is characterized in that: described sericin solution, and its preparation method may further comprise the steps:
(1) will remove Bombyxmori Linnaeus cocoon layer behind the pupa, put into the boiling water processing of coming unstuck;
(2) behind the 30min, stop steaming and decocting, filter roguing;
(3) the sericin solution after will filtering is put into Rotary Evaporators and is concentrated, and obtains the sericin solution of purpose concentration.
3. preparation method according to claim 1 is characterized in that: described hydroxylapatite powder, and its preparation method may further comprise the steps:
(1) gets saturated Ca (OH)
2Solution places 60 ℃ of water-baths, drips the H of 10%wt
3PO
4Solution, and Ca (OH)
2Solution and H
3PO
4The volume ratio of solution is 70: 1～2, during constantly fast stir and keep water temperature;
(2) with the method for dropping ammonia, the pH that adjusts solution remains on 8.7;
(3) drip H
3PO
4Continue to stir 3h behind the solution, stirring finishes to be placed on room temperature and leaves standstill 48h;
(4) the bottom precipitation is collected in the supernatant of inclining; Precipitation is cleaned 2-3 time with dehydrated alcohol earlier, and the reuse distilled water cleans 3-4 time;
(5) precipitation that will obtain is put into refrigerator and cooled and is frozen, and moves in the freeze dryer dryly then, grinds, and obtains hydroxyapatite powder.
4. preparation method according to claim 1 is characterized in that: the weight ratio of sericin and hydroxyapatite is 10: 1～1: 1 in the described step (1).
5. preparation method according to claim 2 is characterized in that: the consumption of water is to the cocoon layer submergence in the described step (1).
6. preparation method according to claim 2 is characterized in that: after also comprising the sericin solution that obtains purpose concentration, extract sericin solution, carry out sds gel electrophoresis, measure the sericin molecular weight.
7. preparation method according to claim 3 is characterized in that: wherein step (1) is to get the saturated Ca of 700ml (OH)
2Solution places 60 ℃ of water-baths, drips the H of 10%wt
3PO
4Solution 11ml, during use magnetic stirring apparatus constantly to stir fast and keep water temperature.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321270A (en) * | 2011-08-22 | 2012-01-18 | 陕西师范大学 | Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent |
| CN102526801A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Preparation method for flexible sericin protein/hydroxyapatite composite membrane |
| CN102532573A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Rapid molding method of sericin/hydroxyapatite composite membrane |
| CN103951831A (en) * | 2014-02-28 | 2014-07-30 | 华中科技大学同济医学院附属协和医院 | Preparation method and application of sericin hydrogel |
| CN106512080A (en) * | 2016-11-24 | 2017-03-22 | 江苏爱西施科技服务咨询股份有限公司 | Preparation method for sericin protein hydrogel |
| CN110665055A (en) * | 2019-07-10 | 2020-01-10 | 赣南医学院第一附属医院 | Sericin/nano-hydroxyapatite tissue engineering bone graft and preparation method and application thereof |
| CN113372131A (en) * | 2021-04-26 | 2021-09-10 | 西安理工大学 | Micro-nano pore channel structure tetragonal phase BaTiO3Preparation method of/HA hollow microspheres |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502672A (en) * | 2009-03-19 | 2009-08-12 | 浙江大学 | Method for preparing hydroxyapatite/silk fibroin compound porous stand material |
-
2010
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502672A (en) * | 2009-03-19 | 2009-08-12 | 浙江大学 | Method for preparing hydroxyapatite/silk fibroin compound porous stand material |
Non-Patent Citations (2)
| Title |
|---|
| AKARI TAKEUCHI ET AL.: "Heterogeneous nucleation of hydroxyapatite on protein: structural effect of silk sericin", 《JOURNAL OF THE ROYAL SOCIETY INTERFACE》 * |
| 金君等: "类牙釉状丝胶蛋白/羟基磷灰石复合材料的合成及表征", 《化学学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321270A (en) * | 2011-08-22 | 2012-01-18 | 陕西师范大学 | Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent |
| CN102321270B (en) * | 2011-08-22 | 2012-12-12 | 陕西师范大学 | Preparation method of silk fibroin/hydroxyapatite/collagen composite porous stent |
| CN102526801A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Preparation method for flexible sericin protein/hydroxyapatite composite membrane |
| CN102532573A (en) * | 2012-02-20 | 2012-07-04 | 浙江大学 | Rapid molding method of sericin/hydroxyapatite composite membrane |
| CN102532573B (en) * | 2012-02-20 | 2013-10-16 | 浙江大学 | Rapid molding method of sericin/hydroxyapatite composite membrane |
| CN103951831A (en) * | 2014-02-28 | 2014-07-30 | 华中科技大学同济医学院附属协和医院 | Preparation method and application of sericin hydrogel |
| CN106512080A (en) * | 2016-11-24 | 2017-03-22 | 江苏爱西施科技服务咨询股份有限公司 | Preparation method for sericin protein hydrogel |
| CN110665055A (en) * | 2019-07-10 | 2020-01-10 | 赣南医学院第一附属医院 | Sericin/nano-hydroxyapatite tissue engineering bone graft and preparation method and application thereof |
| CN110665055B (en) * | 2019-07-10 | 2022-08-26 | 赣南医学院第一附属医院 | Sericin/nano-hydroxyapatite tissue engineering bone graft and preparation method and application thereof |
| CN113372131A (en) * | 2021-04-26 | 2021-09-10 | 西安理工大学 | Micro-nano pore channel structure tetragonal phase BaTiO3Preparation method of/HA hollow microspheres |
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