CN101810249A - Method for producing complex-probiotic group by solid fermentation - Google Patents
Method for producing complex-probiotic group by solid fermentation Download PDFInfo
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- CN101810249A CN101810249A CN201010010060A CN201010010060A CN101810249A CN 101810249 A CN101810249 A CN 101810249A CN 201010010060 A CN201010010060 A CN 201010010060A CN 201010010060 A CN201010010060 A CN 201010010060A CN 101810249 A CN101810249 A CN 101810249A
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Abstract
The invention relates to the field of feed additive, in particular to a method for producing a complex-probiotic group by solid fermentation. The method comprises the specific steps: culturing the complex-probiotic group in a solid fermentation culture medium according to the 3%-4% inoculum size by weight percent, and then carrying out fermentation culture for 72 hours at the temperature of 32-37 DEG C. The culture medium provided by the invention can keep stable reproduction of the complex-probiotic group, can realize high-efficiency fermentation production, simultaneously does not change the functional type metabolin of the complex-probiotic group, can lead the complex-probiotic group to be fermented and produced with high efficiency; and the quantity of the complex-probiotic group after being fermented can be more than 3 billion per gram.
Description
Technical field
What the present invention relates to is feed additive field, the method that a kind of specifically complex-probiotic group by solid fermentation is produced.
Background technology
Probiotics is green feed addictive, can adjust and keep normal microecological balance in the enteron aisle, strengthen body's immunity, promote digesting and assimilating of nutriment, strengthen immunity of organisms, improve feed conversion rate, promote the effect of animal growth and development thereby reach.Because the on a large scale forbidding of antibiotic in feed, the importance of probiotics in animal-breeding improves day by day.
Because therefore the action effect instability of single culture needs the exploitation compound micro-ecological preparation, and supporting exploitation solid culture technology.
Summary of the invention
The purpose of this invention is to provide the method that a kind of complex-probiotic group by solid fermentation is produced.
For achieving the above object, the technical solution used in the present invention is: the method that complex-probiotic group by solid fermentation is produced: the inoculum concentration of 1%-4% is incubated at solid fermentation with complex-probiotic group and cultivates and concentrate by weight percentage, then under temperature 25-37 ℃, fermented and cultured 48-72h.Be preferably: the inoculum concentration of 3%-4% is incubated at solid fermentation with complex-probiotic group and cultivate concentrates by weight percentage, then under temperature 32-37 ℃, and fermented and cultured 72h.Described complex-probiotic group is protein Candida, enterococcus faecalis, lactobacillus acidophilus and/or bacillus subtilis.
Culture medium, by weight percentage, dregs of beans 20%-39%, wheat bran 60%-79%, mineral salt 0.2%-1%.Described mineral salt is magnesium sulfate and ammonium sulfate, counts 1: 9 by weight.
The advantage that the present invention had: culture medium provided by the invention can keep the Steady breed of complex-probiotic group, and can realize the high efficiency fermenting and producing, functional form metabolite to compound probiotic does not change simultaneously, and can make the high efficiency fermenting and producing of complex-probiotic group, fermentation back composite flora beneficial microbe quantity can reach more than 3,000,000,000/g.
The specific embodiment
Embodiment 1
The inoculum concentration of 1%-4% will be bred cultivation according to candida utili, enterococcus faecalis, lactobacillus acidophilus and the bacillus subtilis that conventional method is cultivated in following culture medium separately by weight percentage, then mixing according to percentage by weight is that 3%-4% is inoculated in solid fermentation and cultivate concentrates, then under temperature 25-37 ℃, fermented and cultured 48-72h (referring to table 1, table 2, table 3).
Culture medium is: by weight percentage, and dregs of beans 20%, wheat bran 79%, mineral salt 1%.
Described dregs of beans moisture :≤13.5%, thick protein 〉=43.0%, crude fibre≤5.0%, coarse ash≤6.0%, methionine 〉=0.6%, lysine 〉=2.5%.Mineral salt is magnesium sulfate and ammonium sulfate, counts 1: 9 by weight.。
Described candida utili propagation is incubated at potato and soaks powder (commercial) 3g, and glucose 20g, agar powder 15g, distilled water add in the 1000ml culture medium, and 121 ℃ of autoclavings 20 minutes are standby.
Described enterococcus faecalis propagation is incubated at glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.Described lactobacillus acidophilus propagation is incubated at glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.Described bacillus subtilis propagation is incubated at peptone 10g, agar 20g, and sodium chloride 5g, meat soak powder 10g, and water adds to 1000ml, in the pH7.2 culture medium, 121 ℃ of autoclavings 20 minutes, standby.
Table 1 solid fermentation orthogonal test factor and level
| Level | Bacterium liquid inoculum concentration (%) | Temperature range (℃) | Fermentation time (h) |
| ??1 | ??1～2 | ??25～30 | ??48 |
| ??2 | ??3～4 | ??32～37 | ??72 |
Table 2 solid fermentation orthogonal test scheme and result of the test
| Test group | Bacterium liquid inoculum concentration (%) | Temperature range (℃) | Fermentation time (h) | Viable count (10 9Individual/g) |
| ??1 | ??1～2 | ??25～30 | ??48 | ??6.57 |
| ??2 | ??1～2 | ??32～37 | ??72 | ??8.32 |
| ??3 | ??3～4 | ??25～30 | ??48 | ??9.40 |
| ??4 | ??3～4 | ??32～37 | ??72 | ??17.50 |
| ??5 | ??1～2 | ??32～37 | ??48 | ??6.90 |
| ??6 | ??1～2 | ??25～30 | ??72 | ??8.14 |
| ??7 | ??3～4 | ??32～37 | ??48 | ??15.50 |
| ??8 | ??3～4 | ??25～30 | ??72 | ??16.10 |
Table 3 solid fermentation orthogonal experiments is analyzed
Can be found out that by above-mentioned analysis result the factor primary and secondary that influences the solid fermentation total viable count is followed successively by in proper order: bacterium liquid inoculum concentration, fermentation time and temperature range, desirable fermentation condition are that bacterium liquid inoculum concentration is 3%～4%, 32～37 ℃ of fermentation time 72h, temperature.
Embodiment 2
Difference from Example 1 is: culture medium, by weight percentage, dregs of beans 39%, wheat bran 60%, mineral salt 1%.
Embodiment 3
Difference from Example 1 is: culture medium, by weight percentage, dregs of beans 25%, wheat bran 74.8%, mineral salt 0.2%.
Embodiment 4
Difference from Example 1 is: culture medium, by weight percentage, dregs of beans 30%, wheat bran 69.5%, mineral salt 0.5%.
Claims (5)
1. the complex-probiotic group by solid fermentation method of producing is characterized in that: the inoculum concentration of 1%-4% is incubated at solid fermentation with complex-probiotic group and cultivates and concentrate by weight percentage, then under temperature 25-37 ℃, and fermented and cultured 48-72h.
2. press the method that the described complex-probiotic group by solid fermentation of claim 1 is produced, it is characterized in that: the inoculum concentration of 3%-4% is incubated at solid fermentation with complex-probiotic group and cultivate concentrates by weight percentage, then under temperature 32-37 ℃, fermented and cultured 72h.
3. by the method for claim 1 or 2 described complex-probiotic group by solid fermentation productions, it is characterized in that: described complex-probiotic group is protein Candida, enterococcus faecalis, lactobacillus acidophilus and/or bacillus subtilis.
4. the method for producing by claim 1 or 2 described complex-probiotic group by solid fermentation is characterized in that: described culture medium by weight percentage, dregs of beans 20%-39%, wheat bran 60%-79%, mineral salt 0.2%-1%.
5. by the method for the described complex-probiotic group by solid fermentation production of claim 4, it is characterized in that: described mineral salt is magnesium sulfate and ammonium sulfate, counts 1: 9 by weight.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178044A (en) * | 2011-05-24 | 2011-09-14 | 上海创博生态工程有限公司 | Preparation method of soybean meal fermented feed |
| CN102422987A (en) * | 2011-12-26 | 2012-04-25 | 三门峡龙飞生物工程有限公司 | Xylanase-containing composite probiotic feed additive and preparation method thereof |
| CN103027191A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using bean pulp and bran as protein raw materials and preparation method thereof |
| CN103027184A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials and a preparation method thereof |
| CN103027185A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using corn protein powder and bran as protein raw materials and preparation method thereof |
| CN103027186A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Feeding attracting type fermented feed and preparation method thereof |
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN103621792A (en) * | 2013-11-29 | 2014-03-12 | 吉林省科技评估有限责任公司 | Feed additive containing L-lysine composite probiotics and preparation method thereof |
| CN105132320A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Culture medium and method for cultivating lactobacillus through high-density solid state fermentation |
| WO2021233925A1 (en) * | 2020-05-20 | 2021-11-25 | Société des Produits Nestlé S.A. | Beneficial effect of poaceae and/or leguminoseae fiber on survivability and metabolic activity of bifidobacterium longum |
-
2010
- 2010-01-08 CN CN201010010060A patent/CN101810249A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178044A (en) * | 2011-05-24 | 2011-09-14 | 上海创博生态工程有限公司 | Preparation method of soybean meal fermented feed |
| CN102422987A (en) * | 2011-12-26 | 2012-04-25 | 三门峡龙飞生物工程有限公司 | Xylanase-containing composite probiotic feed additive and preparation method thereof |
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN103027191A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using bean pulp and bran as protein raw materials and preparation method thereof |
| CN103027184A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials and a preparation method thereof |
| CN103027185A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Phagostimulant fermented feed using corn protein powder and bran as protein raw materials and preparation method thereof |
| CN103027186A (en) * | 2012-12-13 | 2013-04-10 | 浙江德清博诚生物科技有限公司 | Feeding attracting type fermented feed and preparation method thereof |
| CN103621792A (en) * | 2013-11-29 | 2014-03-12 | 吉林省科技评估有限责任公司 | Feed additive containing L-lysine composite probiotics and preparation method thereof |
| CN105132320A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Culture medium and method for cultivating lactobacillus through high-density solid state fermentation |
| WO2021233925A1 (en) * | 2020-05-20 | 2021-11-25 | Société des Produits Nestlé S.A. | Beneficial effect of poaceae and/or leguminoseae fiber on survivability and metabolic activity of bifidobacterium longum |
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Application publication date: 20100825 |