CN101806737A - Portable fluorescence detector - Google Patents
Portable fluorescence detector Download PDFInfo
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- CN101806737A CN101806737A CN 201010154808 CN201010154808A CN101806737A CN 101806737 A CN101806737 A CN 101806737A CN 201010154808 CN201010154808 CN 201010154808 CN 201010154808 A CN201010154808 A CN 201010154808A CN 101806737 A CN101806737 A CN 101806737A
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- fluorescence detector
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- portable fluorescence
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Abstract
The invention belongs to fluorescence detectors, in particular to a portable fluorescence detector. The prior art has the defects of large size, inconvenient carrying and unavailable field detection at any time as required and cannot meet the requirements for environment protection, food sanitation and medical detection. In the portable fluorescence detector, a rear shell plate (16) is provided with a USB (universal serial bus) interface (14) and an RS-232 interface (15); a lower shell body (18) is provided with an external power supply interface (17) and a cell box (19); a bottom plate is provided with an LED light source (11), colored glass (5), a focus lens (4), a sample pool (3), a focus lens (6), a color filter (7) and a solid receiver (8); and a panel (21) is provided with an optical filter socket (22), a display screen (9) and a key (10). The invention has the advantages of convenient carrying, low energy consumption, directly read detection result and convenient data transmission, storage and calculation, and can be used for carrying out field detection as required.
Description
Technical field
The invention belongs to a kind of fluoroscopic examination instrument, specifically a kind of portable fluorescence detector.
Background technology
It is the significant problem that is related to mankind itself's living safety that environmental protection, food hygiene, medical science detect, and is the big problem of whole mankind's common concern.Environmental pollution, pollution of waterhead, the uneasy congruence of food produce significant threat to human beings'health.Contain germ, virus, toxic compounds, carcinogen in the food, the frequent generation of incident and potable water, sitotoxismus accident is infected in the propagation that causes the food-borne pathogens of infecting both domestic animals and human, presses for people and invents that a kind of volume is little, easy to carry, on-the-spot to detect sensitive, accurate, fireballing detecting instrument.The fluoroscopic examination of microorganism, toxic compounds has become the detection that must carry out in potable water, food, the Environmental security.When the principle of fluoroscopic examination was the rayed of microorganism or the certain wavelength of compound quilt, they can be stimulated and send fluorescence, measure the fluorescent energy that sends and are fluoroscopic examination.
Prior art fluoroscopic examination instrument is a desk-top instrument, and the shortcoming of prior art fluorescence detector is: volume is big, carries inconvenience, can not do on-the-spot the detection at any time as required; Can't satisfy the needs of environmental protection, food hygiene, medical science detection.Therefore it is crucial inventing sensitive, accurate, the fireballing portable fluorescence detector of little, easy to carry, the on-the-spot detection of a kind of volume.
Summary of the invention
The purpose of this invention is to provide a kind of volume little, easy to carry, detect sensitive, accurate, fireballing portable fluorescence detector.
The object of the present invention is achieved like this:
Portable fluorescence detector is made up of housing, base plate, panel, and housing is divided into upper shell, back casing, lower house; Back casing is provided with USB interface; Lower house is provided with external power interface, battery case; Base plate is located on the lower house, and base plate is provided with led light source, coloured glass, focus lamp, sample cell, focus lamp, color filter, solid receiver; Panel is provided with display screen, button.
Upper shell, lower house connect as a whole by connecting screw.
The top of upper shell is connected with cover plate, and cover plate is positioned at the top of optical filter socket.
Main points of the present invention are:
1, adopting low-power consumption high brightness monochromatic LED is light source: replace the exciter lamp source of prior art, the fluorescence detector volume is reduced greatly, reduced power consumption simultaneously.
2, the highly sensitive solid receiver of utilization substitutes the photomultiplier of prior art, is beneficial to be convenient for carrying.
3, anti-interference preposition amplification design, the detection sensitivity height.
4, use the USB flash disk Rapid storage technique, can reach PC to data and store and calculate, greatly facilitate carrying and using of portable fluorescence detector.
5, panel is provided with display screen, can directly read testing result.
6, housing is provided with battery case and power supply adaptor interface, makes this detector can alterating and direct current dual-purpose, convenient on-the-spot the detection.
The principle of fluorescence detector of the present invention is: fluorescence be that material radiates the long fluorescence of wavelength after extinction, the fluorescence efficiency of fluorescent material is relevant in the extinction degree of fluorescence intensity of solution and this solution and the solution.The extinction degree of solution depends on the content of fluorescent material under certain condition, so can measure the content of material by fluorescence intensity.The light beam that goes out when radiation of light source is through shining behind the exciter filter on the sample, and the fluorescent material in the sample absorbs emitting fluorescence behind the exciting light, and fluorescence shines on detecting device after being filtered by focus lamp optically focused and emission optical filter, converts light signal to electric signal.Deliver to microprocessor through the signal amplification circuit amplification and handle, and then offer the user in the mode of numeral demonstration and printing.
Fluorescence detector is because the constraint of the sensitivity of be stimulated light source and receiver, it is excitation source that the prior art fluorescence detector is all used xenon lamp, deuterium Deng, mercury lamp, with photomultiplier is receiver, and this is that signal is very little because fluoroscopic examination mostly is to do trace analysis.The present invention adopts the LED of high brightness to do light source, adopts the solid photelectric receiver, and high strength led light source and hypersensitive detection technique of fluorescence are combined, and has invented portable fluorescence detector.Battery of the present invention, power supply AC/DC; instrument has rs-232 mouth and u dish delivery outlet, can not be subjected to power limitations, does on-the-spot the detection and lab analysis at any time; directly draw testing result, the scene in fields such as instant food detection greatly, environmental protection, clinical medicine is detected.Advantages such as portable fluorescence detector of the present invention has simply, quick, accurate, cheap, easy to detect, processing ease.
Excitation wavelength of the present invention: 470nm; Wavelength of transmitted light: 520nm.Display window: 128X64LCD; Data output: RS-232, USB flash disk; The sample cell frame: 10x10mm standard colorimetric pool frame, also replaceable is φ 13mm test tube pond frame.Power requirement: 1.5X4AAA alkalescence rechargable battery, perhaps DC6V 1A adapter.
The present invention is a kind of portable fluorescence detector, can be widely used in industries such as food inspection, environmental protection, clinical medicine, can be combined into multi-purpose detecting device with technology such as fluorescent dye, quantum dot fluorescence, fluorescence probes.This instrument is combined with coloured glass and focus lamp by the LED of low-power consumption forms excitation light path, and the monochromatic light of certain bandwidth is shone on the sample cell, is passed on the receiver by the fluorescence of emission light path with sample cell again, and the emission light path comprises focal length mirror and interference filter.Sample cell can have the standard cuvette, also can be the 200ul miniature tube.Portable photofluorometer has rs-232 mouth and u dish delivery outlet, and data result is shown by display screen, also can copy by inserting USB flash disk by serial line interface RS-232 output; Can reach PC to data and carry out any storage and calculating.
Optical system of the present invention comprises with the lower part:
Light source: the present invention adopts long-term durability luminous diode to do light source, and in order to prolong the serviceable life of light source, instrument only uses the LED fluorescent tube when measuring, and arranged between each sample measurement the interval time in 2 seconds.
Coloured glass: form monochromatic light as exciting light with colored light sources.
Focus lamp: above-mentioned light beam focuses on the sample after passing focus lamp.
Optical filter: the fluorescence that sample sends is by high permeability optical filter 520nm.
Focus lamp: fluorescent light beam focuses on photelectric receiver by focus lamp.
Photelectric receiver: arrive photelectric receiver by focus lamp, by the photoelectric receiver measuring intensity of fluorescence.
Keyboard: the F1 of keyboard, F2, F3, F4 can be according to the corresponding difference in functionalitys in different interfaces.
Sample cell frame: 10x10mm standard colorimetric pool frame or 200ul trace pipe support.
Photofluorometer volume of the present invention is little, in light weight, is convenient for carrying; Be applicable to a lot of fluorescence probes mensuration, can be used to nucleic acid and albumen are carried out quantitatively that test item is decided according to the matched reagent box.
Condition of work of the present invention is: place on the smooth and steady workstation, sunlight and light direct projection must not be arranged, must not have the intense electromagnetic field to disturb; Temperature 10-30 ℃.
This instrument has two kinds of working methods: 1, main frame+external power adapter; 2, main frame includes 4 joint 1.5AAA alkaline batteries.
Adaptor power supplies: input: AC 100-240V 0.3A 50/60Hz; Output :+6V 2A; Main frame input :+6V 0.5A.Battery supply: No. 7 AAA 1.5V alkaline batteries of 4 joints; Mains fluctuations do not exceed nominal voltage ± 10%.
Advantage of the present invention is:
1, volume is little, and is easy to carry.
2, energy consumption is low.
3, detection sensitivity height.
4, testing result display digit is immediately directly read.
5, have rs-232 mouth and u dish delivery outlet, data pass transmission, storage and convenience of calculation.
6, detecting operation simply, easily and fast, accurately.
7, can carry out the scene at any time and detect as required.
8, low price.
Description of drawings
Fig. 1 is a portable fluorescence detector structural map of the present invention.
Fig. 2 is a portable fluorescence detector outside drawing of the present invention.
Among the figure: 1 is housing; 2 is base plate; 3 is sample cell; 4 is focus lamp; 5 is coloured glass; 6 is focus lamp; 7 is optical filter; 8 is solid receiver; 9 is display screen; 10 is button; 11 is led light source; 12 is cover plate; 13 is upper shell; 14 is the USB socket; 15 is RS-232 interface; 16 is back casing; 17 is the power supply adaptor socket; 18 is lower house; 19 is battery case; 20 is connecting screw; 21 is panel; 22 is the optical filter socket.
Embodiment
Below by embodiment to this bright being described further.
Embodiment 1:
Portable fluorescence detector, physical dimension: 180mmx84mmx70mm; Weight: 0.5Kg.Serviceability temperature scope: 10 ℃-30 ℃; Transport temperature scope :-40 ℃-+55 ℃; Storage temperature range: 0 ℃-+40 ℃.Using maximum relative humidity is 70%.The main frame hand-held; Adapter is direct insertion; The method of operation: operation continuously.
Portable fluorescence detector is made up of housing 1, base plate 2, panel 21, and housing 1 is divided into upper shell 13, back coverboard 16, lower house 18.Base plate 2 is connected with lower house 18, and base plate 2 is provided with led light source 11, coloured glass 5, focus lamp 4, sample cell 3, focus lamp 6, color filter 7, solid receiver 8; Panel 21 is provided with optical filter socket 22,9,4 buttons 10 of display screen.Back coverboard 16 is provided with usb 14, RS-232 interface 15.Lower house 18 is provided with external power interface 17, the adapted adaptor power supplies: input: AC 100-240V 0.3A 50/60Hz; Output: ...+6V 2A; The main frame input: ...+6V 0.5A.Lower house 18 also is provided with battery case 19; Adorn No. 7 battery AAA 1.5V alkaline batteries of 4 joints in the battery case.Upper shell 13, lower house 18 connect as a whole by connecting screw 20.The top of upper shell 13 is connected with cover plate 12, and cover plate 12 is positioned at the top of optical filter socket 22.The cover plate 12 that closes, led light source 11, coloured glass 5, focus lamp 4, sample cell 3, color filter 7, solid receiver 8 are housed in the cover plate 12, the lucifuge protection.
The present invention starts shooting and uses preheating time: 5 seconds.Excitation wavelength: 470nm; Wavelength of transmitted light: 520nm.Display window: 128X64LCD; Interface: RS-232; Data output: RS-232, USB flash disk.Sample cell frame: 10x10mm standard colorimetric pool frame or 200ul trace pipe support.
Instrument of the present invention is pressed the GB4793.1-2007 safety standard and is carried out.Safety indexes is under 2000 meters conditions of sea level elevation, and temperature is lower than 31 ℃, and maximum relative humidity is that operation in 80% o'clock also can meet safety standard.
Embodiment 2:
Water sample detection, step is as follows:
1, detection was calibrated fluorophotometer the same day:
(1) is equipped with zeroing liquid: select blank cuvette for use, add the 3ml pure water;
(2) be equipped with calibration solution: select another empty cuvette for use, add 3ml pure water, 20 μ lDNA-fluorescent dye mixings.
(3) calibration fluorophotometer: open instrument ON/OFF switch, instrument master menu (Main Menu) occurs:
Enter " Read Sample " window by F1; By entering " ReadSample " window once more after the F1 adjusting sensitivity; Press F2 CAL key, press the display screen indication and insert zeroing cuvette (BLANKcuvette) and press READ.Take out zeroing cuvette substitution CAL cuvette; Press READ once more, this moment, instrument calibration finished; The fluorescence reading is 1,000AU ± 5%.
2, detect the toxicity of water sample:
(1) get the sky cuvette, add 2ml Baxter pure water, and 20 μ l DNA-fluorescent dye, mixing and cover lids.This cuvette can be used to open-air scene immediately and detect.
(2) add the water sample of 1ml, reverse and mix for 5 times.
(3) cuvette that will contain water sample inserts fluorophotometer, with figure notation towards last.
(4) close the fluorophotometer lid, and press the READ key.
(5) in one minute, guarantee not have the drift of reading for 5 times by the READ key; Note down this reading.
(6) display screen shows, pattern detection result is: 99.
The above is the preferred embodiments of the present invention only, is not limited to the present invention, and for a person skilled in the art, the present invention can have change and change.Within the spirit and principles in the present invention all, any modification of being done, improvement etc. all should be included within protection scope of the present invention.
Claims (3)
1. a portable fluorescence detector is made up of housing (1), base plate (2), panel (21), it is characterized in that: housing (1) is divided into upper shell (13), back coverboard (16), lower house (18); Back coverboard (16) is provided with USB interface (14), RS-232 interface (15); Lower house (18) is provided with external power interface (17), battery case (19); Base plate (2) is connected with lower house (18), and base plate (2) is provided with led light source (11), coloured glass (5), focus lamp (4), sample cell (3), focus lamp (6), color filter (7), solid receiver (8); Panel (21) is provided with optical filter socket (22), display screen (9), button (10).
2. portable fluorescence detector according to claim 1 is characterized in that: upper shell (13), lower house (18) connect as a whole by connecting screw (20).
3. portable fluorescence detector according to claim 1 is characterized in that: the top of upper shell (13) is connected with cover plate (12), and cover plate (12) is positioned at the top of optical filter socket (22).
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010154808 CN101806737A (en) | 2010-04-23 | 2010-04-23 | Portable fluorescence detector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010154808 CN101806737A (en) | 2010-04-23 | 2010-04-23 | Portable fluorescence detector |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495036A (en) * | 2011-12-07 | 2012-06-13 | 长春美泰科技有限公司 | On-site detector for fluorescent bleaching agent in living paper products |
| CN103115911A (en) * | 2013-03-05 | 2013-05-22 | 中国原子能科学研究院 | Closed type fluorescence analyzer |
| CN103278484A (en) * | 2013-05-11 | 2013-09-04 | 徐培实 | Portable fluorescent brightener detector |
| CN103558192A (en) * | 2013-08-13 | 2014-02-05 | 未名兴旺***作物设计前沿实验室（北京）有限公司 | Apparatus for detecting red fluorescent protein seed |
| WO2014032615A1 (en) * | 2012-08-31 | 2014-03-06 | Bao Diana | Portable metal ion detector |
| CN104614071A (en) * | 2014-12-09 | 2015-05-13 | 浙江福立分析仪器有限公司 | Miniature optical path structure of spectrophotometer and application method thereof |
| CN105372215A (en) * | 2014-09-02 | 2016-03-02 | 什邡市祥力机电有限公司 | Pesticide residue quantity detecting device and method |
| CN105368709A (en) * | 2015-11-25 | 2016-03-02 | 中国科学院长春光学精密机械与物理研究所 | Detection device for tuberculosis diagnosis |
| CN106770091A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of isothermal portable fluorescence detector for detecting coronary heart disease |
| CN107328749A (en) * | 2017-08-31 | 2017-11-07 | 河海大学 | A kind of water quality fluorescent solutions apparatus for measuring concentration and measuring method |
| CN107817231A (en) * | 2017-10-13 | 2018-03-20 | 浙江清华长三角研究院 | A kind of portable detector for being directed to coliform and EHEC |
| CN109060742A (en) * | 2018-08-06 | 2018-12-21 | 张家林 | A kind of Portable thermal circulation fluorescence detector |
| CN109307666A (en) * | 2018-11-21 | 2019-02-05 | 清华大学 | A kind of Portable fluorescence spectral detection system and the method using system differentiation recycled water and drinking water |
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Patent Citations (4)
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| US20040126279A1 (en) * | 2002-08-02 | 2004-07-01 | Renzi Ronald F. | Portable apparatus for separating sample and detecting target analytes |
| CN101038256A (en) * | 2007-04-03 | 2007-09-19 | 西安天隆科技有限公司 | Hand-held adenosine triphosphate fluorescent detecting instrument |
| CN101231244A (en) * | 2008-02-15 | 2008-07-30 | 中国科学院上海微***与信息技术研究所 | Fluorescence detection micro-current control biochemical analyzer evoked by high-power LED |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495036A (en) * | 2011-12-07 | 2012-06-13 | 长春美泰科技有限公司 | On-site detector for fluorescent bleaching agent in living paper products |
| CN104995499A (en) * | 2012-08-31 | 2015-10-21 | D·包 | Portable metal ion detector |
| WO2014032615A1 (en) * | 2012-08-31 | 2014-03-06 | Bao Diana | Portable metal ion detector |
| CN103115911A (en) * | 2013-03-05 | 2013-05-22 | 中国原子能科学研究院 | Closed type fluorescence analyzer |
| CN103278484A (en) * | 2013-05-11 | 2013-09-04 | 徐培实 | Portable fluorescent brightener detector |
| CN103558192A (en) * | 2013-08-13 | 2014-02-05 | 未名兴旺***作物设计前沿实验室（北京）有限公司 | Apparatus for detecting red fluorescent protein seed |
| CN105372215A (en) * | 2014-09-02 | 2016-03-02 | 什邡市祥力机电有限公司 | Pesticide residue quantity detecting device and method |
| CN104614071A (en) * | 2014-12-09 | 2015-05-13 | 浙江福立分析仪器有限公司 | Miniature optical path structure of spectrophotometer and application method thereof |
| CN105368709A (en) * | 2015-11-25 | 2016-03-02 | 中国科学院长春光学精密机械与物理研究所 | Detection device for tuberculosis diagnosis |
| CN106770091A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of isothermal portable fluorescence detector for detecting coronary heart disease |
| CN107328749A (en) * | 2017-08-31 | 2017-11-07 | 河海大学 | A kind of water quality fluorescent solutions apparatus for measuring concentration and measuring method |
| CN107817231A (en) * | 2017-10-13 | 2018-03-20 | 浙江清华长三角研究院 | A kind of portable detector for being directed to coliform and EHEC |
| CN109060742A (en) * | 2018-08-06 | 2018-12-21 | 张家林 | A kind of Portable thermal circulation fluorescence detector |
| CN109060742B (en) * | 2018-08-06 | 2021-10-26 | 张家林 | Portable thermal cycle fluorescence detector |
| CN109307666A (en) * | 2018-11-21 | 2019-02-05 | 清华大学 | A kind of Portable fluorescence spectral detection system and the method using system differentiation recycled water and drinking water |
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Open date: 20100818 |