CN101805369A - Preparation methods of distearoyl phosphatidyl ethanolamine and amino polyethylene glycol derivatives thereof - Google Patents
Preparation methods of distearoyl phosphatidyl ethanolamine and amino polyethylene glycol derivatives thereof Download PDFInfo
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Abstract
The invention discloses preparation methods of distearoyl-phosphoethanolamine and amino polyethylene glycol derivatives thereof and relates to effective preparation methods of a medicinal carrier material phospholipids-distearoyl-phosphoethanolamine (DSPE for short) and amino polyethylene glycol derivatives of the distearoyl-phosphoethanolamine (NH2-PEG-DSPE for short). The preparation method of the distearoyl-phosphoethanolamine comprises the following steps of: firstly reacting phosphorus oxychloride with 1,2-distearoyl-glyceride to obtain phosphorylcholine monoester which is I for short; reacting the phosphorylcholine monoester with 2-hydroxyethyl-benzyqcarbamate to obtain DSPE containing amino-protecting group which is II for short; and carrying out catalytic hydrogenation on the II to remove the protecting group to obtain DSPE. The preparation method of the amino polyethylene glycol derivatives of the distearoyl-phosphoethanolamine comprises the following steps of: reacting DSPE with nitrine substituted PEG chloro-carbonic ester which is VI for short to obtain nitrine-polyethylene glycol DSPE derivatives which is III for short; and reducing the nitrine into amino by catalytic hydrogenation to obtain the amino polyethylene glycol derivatives of the distearoyl-phosphoethanolamine.
Description
Technical field
The present invention relates to a kind of medicinal carrier material phosphatide--(letter is NH for-methods of distearoyl phosphatidyl ethanolamine (letter is represented for DSPE) and amino polyethylene glycol derivatives thereof
2-PEG-DSPE represents) effective preparation method.
Background technology
Liposome is a kind of application medicinal carrier material very widely, it can form unique phospholipid bilayer orientation structure, both hydrophilic medicament can be encapsulated in interior water, lipophilic drugs can be encapsulated in phospholipid bilayer again, the lipid physical efficiency effectively delays drug release after the intravenous injection, improves bioavailability.In addition, liposome and microbial film consistency are good, and toxicity is low.Axunge plastid material is made up of phosphatide and additives usually, and wherein phosphatide is the main component that constitutes liposome physics and biological nature.
Methods of distearoyl phosphatidyl ethanolamine (DSPE) is the phosphatide a kind of commonly used of preparation liposome, and on chemical structure, it comprises a hydrophilic thanomin end group and a lipophilic stearic acid triglyceride tail base.Different with natural phospholipids such as Yelkin TTS, fabaceous lecithin, kephalins, methods of distearoyl phosphatidyl ethanolamine is artificial synthetic phospholipid, has advantages such as chemical ingredients is definite, quality controllable.The methods of distearoyl phosphatidyl ethanolamine terminal amino group linked to each other with polyoxyethylene glycol (PEG) by covalent linkage can obtain PEGization phosphatide, the liposome that application PEGization phosphatide prepares is commonly called long circulating liposomes, because the PEG fragment can be induced at molecular surface in vivo and be formed a moisture film, can effectively slow down in the serum Opsonin and reticuloendothelial system to the picked-up and the removing of liposome, thereby prolong liposome transformation period in the recycle system in vivo.The pertinent literature report of domestic at present, external visible Pegylation phosphatide (PEG-DSPE) and methoxy poly (ethylene glycol) phosphatide (m-PEG-DSPE), but yet there are no amino polyethylene glycol phosphatide (NH
2-PEG-DSPE) the open report of synthetic method.
Though DSPE has used for many years, its synthesis technique report is relatively limited.Generally, the DSPE synthetic route of bibliographical information at first is by selective protection glycerine terminal hydroxyl, prepares 1 through the acidylate deprotection, 2-distearyl glyceride (Chem.Phys.Lipids, 1980,26,405; Chem.Phys.Lipids, 1986,41,53; Tetrahedron Lett., 1989,30,3585), then with 1, the thanomin of 2-dialycerides, thanomin or band amino protecting group successively makes corresponding phospholipids with the phosphorylating agent reaction.In this route, can be directly and phosphorylating agent such as phosphorus oxychloride reaction (PNAS, 1978 with thanomin, dialycerides; 75; 4074), but the general reactive behavior height of phosphorylating agent, poor selectivity, cause this reaction to have that by product is many, separation and purification is difficult and defective such as yield is low.Therefore, phosphorylating agent or thanomin protection are become preferably select in advance, wherein the selection of blocking group is comparatively crucial for this.
The phosphorylating agent of report mainly contains at present: 1) phosphorus oxychloride.This reagent is the most classical, have raw material be easy to get, active high and react characteristics such as fast, but because three active chlorine atoms are arranged, two acidylate side reactions easily take place in the first step acylation process, cause product yield to reduce (Arch.Biochem.Biophys., 1958,78,294; HelyChim.Acta, 1991,74,1697; J.Org.Chem., 1996,61,192).2) phosphoryl chloride list (two) ester.For fear of many acidylates side reaction that phosphorus oxychloride had, phosphoryl chloride list (two) ester also is often used as phosphorylating agent, and common have (J.Org.Chem., 1977,42,2260. such as two chlorethoxyfos phenylesters, two chlorethoxyfos benzyl esters, phosphoryl chloride diphenyl ester; J.Med.Chem., 1990,33,641; J.Org.Chem., 2002,67,194).Such reagent can effectively be avoided the side reaction of many acidylates, and reaction conditions is comparatively gentle, yet compares with phosphorus oxychloride, and its source is limited on the one hand, also need add a step de-ester reaction on the other hand.3) inferior phosphoryl chloride monoesters.When dialycerides and pure amine structure were very huge, phosphoryl chloride can't react with it sometimes, adopts inferior phosphoryl chloride monoesters can effectively promote reactive behavior, generate corresponding inferior phosphoramide, obtain target phosphatide (Tetrahedron, 1992 through oxidation then, 48,2223; J.Org.Chem., 1994,59,4805).Such reagent can effectively be avoided the side reaction of many acidylates, but reaction relates to oxidation, is unfavorable for preparing the compound that contains oxidation-sensitive, also need increase by a step de-ester reaction in addition.4) phosphoryl chloride ring second diester.This reagent and dialycerides reaction can directly obtain glycerine-thanomin phosphatide (J.Org.Chem., 1999,64,7727) after ammonia is separated, and its reaction conditions is comparatively gentle, yet it is limited to originate, and cost is higher.
Selection to thanomin amido protecting group also has different reports at present: 1) tertbutyloxycarbonyl (Boc).This protecting group is to report maximum protecting groups at present, and its reaction is simple, and mild condition, shortcoming are that cost is higher relatively, and. deprotection needs acidic conditions also can exert an influence to some specific groups.2) trichloro-ethoxycarbonyl.As amino protecting group, it reacts equally rapidly and efficiently, and can slough protection (J.Med.Chem., 1975,18,1184) under the Zn/ acetic acid treatment.In addition, use ethylene bromohyrin when also being reported in preparation glycerine-thanomin phosphatide, after phosphorylated obtains phosphatide, replace bromine with ammoniacal liquor again and can obtain target product (Chem.Phy.Lipids, 1978,22,1) as raw material.But above-mentioned protecting group has a common shortcoming, is exactly that reaction process is difficult to follow the tracks of.
In the DSPE preparation technology who has reported, very high to the low temperature requirement mostly, the product productive rate is lower, and cost is also higher, is difficult to be applied to suitability for industrialized production.On the basis of comprehensive relatively more existing several different methods relative merits, the present inventor has researched and developed the synthesis technique of a kind of simple and effective ground preparation DSPE, and it is linked to each other with polyoxyethylene glycol, obtains NH through further deriving
2-PEG-DSPE.
Summary of the invention
Technical problem: the purpose of this invention is to provide a kind of methods of distearoyl phosphatidyl ethanolamine (DSPE) and amino polyethylene glycol derivatives (NH thereof
2-PEG-DSPE) preparation method.
Technical scheme: the synthetic route of DSPE involved in the present invention is carried out through a, b, c three-step reaction according to shown in the reaction formula 1; At first with phosphorus oxychloride and 1; the reaction of 2-distearyl glyceride obtains phosphinylidyne dichloro monoesters (letter is represented for I); secondly I and the effect of 2-hydroxyethylamino benzyl formate are obtained containing the DSPE (simple represent for II) of amino protecting group, then the II catalytic hydrogenation is sloughed protecting group and obtain DSPE.
Reaction formula 1
In a step reaction, under the nitrogen protection, a certain amount of alkali is dissolved in the anhydrous aprotic solvent, drip a certain amount of phosphorus oxychloride and 1 under 0 ℃ respectively, the solution of the above-mentioned solvent of 2-distearyl glyceride is then 0 ℃ of reaction, gained I reaction solution is directly used in b step reaction without separation; Wherein, alkali is tertiary amine, can be triethylamine, Tributylamine, pyridine or N, accelerine, preferred triethylamine; Aprotic solvent is chloroform, methylene dichloride, N, dinethylformamide or tetrahydrofuran (THF), preferred tetrahydrofuran (THF); A certain amount of phosphorus oxychloride, 1, the molar ratio of 2-distearyl glyceride and alkali are 0.9-1.3: 1: 1, preferred proportion was 1.2: 1: 1, and the phosphorus oxychloride of too high or too low amount can cause byproduct of reaction to increase or react not exclusively; Because exothermic heat of reaction drips phosphorus oxychloride and 1, should carry out below 0 ℃ during the solution of 2-distearyl glyceride, too high temperature can cause the increase of side reaction, and low excessively temperature then causes unnecessary energy consumption.
In b step reaction, under the nitrogen protection, with the reaction solution of a step reaction gained I, below 10 ℃, drip the solution of the anhydrous aprotic solvent of a certain amount of 2-hydroxyethylamino benzyl formate and alkali, in room temperature reaction, add water then and stop, obtain II through aftertreatment and column chromatography for separation; Wherein, alkali is tertiary amine, can be triethylamine, Tributylamine, pyridine or N, accelerine, preferred triethylamine; Aprotic solvent is chloroform, methylene dichloride, N, dinethylformamide or tetrahydrofuran (THF), preferred tetrahydrofuran (THF); The molar ratio of a certain amount of 2-hydroxyethylamino benzyl formate, alkali and I (calculated amount) is 1: 1: 1, and too high or too low ingredient proportion will cause the increase and the isolating difficulty of by product; Because exothermic heat of reaction should carried out below 10 ℃ when the thanomin of dropping carbobenzoxy-(Cbz) protection and the solution of alkali, too high temperature can cause the increase of side reaction, and low excessively temperature then causes unnecessary energy consumption; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and wherein methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride; Alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
In c step reaction, II dissolves in a certain proportion of alkyl alcohol and the methyl chloride mixed solvent with b step reaction gained, adds catalyzer, and carbobenzoxy-(Cbz) is sloughed in hydrogenation under the hydrogen of certain pressure and temperature of reaction, obtains product D SPE through aftertreatment then; Wherein, the alkyl alcohol in the mixed solvent is methyl alcohol or ethanol, and methyl chloride is methylene dichloride or chloroform, the mixed solvent that preferred alcohol and methylene dichloride are formed; The ratio of alkyl alcohol and methyl chloride is at 1-3 in the mixed solvent: between 1, preferred proportion is 1: 1; Catalyzer is palladium carbon or Raney nickel, preferred 10% palladium carbon.
NH involved in the present invention
2The synthetic route of-PEG-DSPE is carried out through d and e two-step reaction according to shown in the reaction formula 2; At first PEG chloro-formic ester (letter is represented for the VI) reaction of DSPE and nitrine replacement is obtained the DSPE derivative (letter is represented for III) of azido-Pegylation, by catalytic hydrogenation azido-is reduced to amino then and obtains NH
2-PEG-DSPE.
Reaction formula 2
In d step reaction, in the presence of alkali, VI is dissolved in the anhydrous methyl chloride, with equimolar DSPE reaction, obtain III through aftertreatment and column chromatography for separation; Wherein, alkali is tertiary amine, can be triethylamine, Tributylamine, pyridine or N, accelerine, preferred N, accelerine; The methyl chloride solvent is chloroform or methylene dichloride, preferred methylene dichloride; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and wherein methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride; Alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
In e step reaction, III dissolves in a certain proportion of alkyl alcohol and the methyl chloride mixed solvent with d step reaction gained, adds catalyzer, and hydrogenation under the hydrogen of certain pressure and temperature of reaction obtains product NH through aftertreatment
2-PEG-DSPE; Wherein, the alkyl alcohol in the mixed solvent is methyl alcohol or ethanol, and methyl chloride is methylene dichloride or chloroform, the mixed solvent that preferred alcohol and methylene dichloride are formed; The ratio of alkyl alcohol and methyl chloride is at 1-3 in the mixed solvent: between 1, preferred proportion is 1: 1; Catalyzer is palladium carbon or Raney nickel, preferred 10% palladium carbon.
The PEG chloro-formic ester that the employed nitrine of d step reaction replaces in the reaction formula 2 can be according to shown in the reaction formula 3, through f and the preparation of g two-step reaction; At first PEG-tosic acid monoesters (letter is represented for IV) and sodiumazide are carried out substitution reaction and obtain the PEG (letter is represented for V) that single azido-replaces, then it and triphosgene reaction are obtained corresponding chloro-formic ester (letter is represented for VI).
Reaction formula 3
In f step reaction, a certain amount of IV and sodiumazide are dissolved in N, react in the dinethylformamide, obtain V through aftertreatment and column chromatography for separation; Wherein, the molar ratio of a certain amount of IV and sodiumazide is 1: 1-3, preferred proportion are 1: 2, and sodiumazide low excessively or a large amount excessively can cause reacting not exclusively or the waste of reactant; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and wherein methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride; Alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
In g step reaction, in the presence of alkali, a certain amount of V is dissolved in the mixed solvent of anhydrous methyl chloride and anhydrous alkylbenzene, with the triphosgene effect, obtain VI through aftertreatment; Wherein, alkali is tertiary amine, can be triethylamine, Tributylamine, pyridine or N, accelerine, preferred N, accelerine; The molar ratio of a certain amount of V, alkali and triphosgene is 1: 1: 1-3, preferred proportion are 1: 1: 3; The methyl chloride solvent is methylene dichloride or chloroform, preferred methylene dichloride; Alkyl benzene solvent is toluene, ethylbenzene or dimethylbenzene, preferred toluene.
Raw material used in the DSPE synthesis technique of the present invention is cheap and easy to get, reaction conditions gentleness, and easy handling.Because carbobenzoxy-(Cbz) for the protecting group of bibliographical information, has significant uv-absorbing, can use thin-layer chromatography to follow the tracks of reaction, the control reaction process.What is more important owing to adopted the amino of carbobenzoxy-(Cbz) protection thanomin, can improve the selectivity of reaction effectively.In addition meaningfully, raw material 1,2-distearyl glyceride can be after reaction can partially recycled usefulness again, reduced raw materials cost.
NH of the present invention
2Technology related in the-PEG-DSPE synthesis technique is simple relatively; mild condition; raw materials usedly replace and obtain, both improved the selectivity of triphosgene acylation reaction, can introduce the terminal amino group of target product again easily by hydro-reduction by PEG being carried out single azido-.
Infrared spectra, nucleus magnetic hydrogen spectrum and mass spectrum have been determined the molecular structure of compound by the compound of the inventive method preparation.
Embodiment
The present invention obtains further instruction by following embodiment, but these explanations are not restriction the present invention.
The preparation of embodiment 1.2-hydroxyethylamino benzyl formate
The 12mL thanomin is mixed in the mixed solvent (1: 1) of 300mL dioxane and water, add 100mg N respectively, accelerine, 32mL Carbobenzoxy Chloride and 28mL triethylamine, mixed solution stirring at room 12h, removal of solvent under reduced pressure obtains viscous solution then.It with 200mL methylene dichloride and the dilution of 100mL water, is separated organic layer, and water layer is used dichloromethane extraction (50mL * 3) again.Merge organic layer, wash respectively 2 times with aqueous hydrochloric acid, water, the saturated aqueous common salt of 0.1M, anhydrous sodium sulfate drying filters, concentrate, the white crude product of 37.0g.The gained crude product with 65mL petrol ether/ethyl acetate mixed solvent (40mL ethyl acetate, 25mL sherwood oil) heating for dissolving, is got milky white solution, and cooling crystallization gets the 34.6g product, yield 91%.
The preparation of embodiment 2. Compound I I
In being inserted with the 1000mL drying three-necked bottle of thermometer, after adding the dry anhydrous tetrahydrofuran (THF) of 250mL down, be cooled to 0 ℃, nitrogen protection adds the 3.6g triethylamine, and slow then the dropping contains 0.34g POCl
3The 50mL anhydrous tetrahydrofuran solution, remain on 0 ℃ and continue down to stir 0.5h.With 18.6g 1,2-distearyl glyceride is dissolved in the 250mL anhydrous tetrahydro furan, then it is slowly dropped in the above-mentioned reaction solution, and the control reacting liquid temperature is not higher than 0 ℃, and about 2.5h finishes.Continue to stir 1h, stirred overnight at room temperature then down at 0 ℃.Below 10 ℃, anhydrous tetrahydrofuran solution (100mL) to the slow Dropwise 5 .8g of above-mentioned reaction solution 2-hydroxyethylamino benzyl formate and 3.6g triethylamine, behind the stirring at room reaction 24h, add 15mL water termination reaction again, the pressure reducing and steaming organic solvent, resistates with 1% hcl acidifying to pH=1, dichloromethane extraction (50mL * 3), anhydrous sodium sulfate drying, concentrating under reduced pressure, column chromatography (methylene dichloride: sherwood oil: methyl alcohol=10: 1: 1), reclaim earlier and obtain raw material 1,2-distearyl glyceride 5.6g, the rate of recovery about 30%; Separate then white wax shape product 10.2g, yield 40%.
IR(v，cm
-1)：3360s(-OH)，2917vs(Alkyl)，1740vs(O＝C-O)，1690s(O＝C-NH)，1537s(Ar)，1468vs(Ar)，1255vs(COO-C)；ESI-MS(m/z)：880[M-H]
-；
1H-NMR(300MHz，CDCl
3)：δ＝7.35-7.26(m，5H，Ar)，5.75(br，1H，N
H)，5.19(s，2H，Ar-C
H 2 )，5.11-5.07(m，1H，CH
2-C
H-CH
2)，4.11-4.01(m，4H，O-C
H 2 -C
H 2 -O)，3.73-3.62(m，2H，OP-OCH
2C
H 2 )，3.39-3.48(m，2H，OP-OC
H 2 )，2.24(br，4H，(C
H 2 CO)
2)，1.55(br，4H，(C
H 2 CH
2CO)
2)，1.41-1.12(m，56H，alkyl)，0.88(t，J＝6Hz，6H，C
H 3 )。
The preparation of embodiment 3.DSPE
1.9g II is joined in 600mL ethanol/dichloromethane (1: the 1) mixed solvent, be heated to 50 ℃ and get colorless cleared solution, add 0.5g 10% palladium carbon then, under 10 normal atmosphere hydrogen, in 50 ℃ of reaction 4h.After reaction finishes, filtered while hot, filtrate is concentrated into 150mL, the adularescent solid is separated out, 3h is left standstill in cooling, filter crude product.With ethanol/dichloromethane mixed solvent recrystallization, get white solid 1.3g (yield 82%).
IR(v，cm
-1)：3263s(-NH)，2917vs(Alkyl)，1737vs(O＝C-O)，1627s(-NH)，1265vs(COO-C)；ESI-MS(m/z)：746[M-H]
-，748[M+H]
+；
1H-NMR(300MHz，CDCl
3)：δ＝5.20-5.17(m，1H，CH
2-C
H-CH
2)，4.12-3.99(m，4H，O-C
H 2 -C
H 2 -O)，3.48-3.27(m，2H，OP-OC
H 2 )，3.14-3.08(m，2H，OP-OC
H 2 )，2.30(br，4H，(C
H 2 CO)
2)，1.58(br，4H，(C
H 2 CH
2CO)
2)，1.41-1.12(m，56H，alkyl)，0.88(t，J＝6Hz，6H，C
H 3 )。
The preparation of embodiment 4. compound IV
With 0.68g Tosyl chloride, 5.00g PEG2000 and 0.31gN, accelerine joins in the 25mL anhydrous methylene chloride, and backflow 3h is cooled to room temperature then, with 5%HCl solution washing reaction solution (25mL * 2), separate organic layer, anhydrous sodium sulfate drying filters, concentrate, column chromatography (methylene dichloride: sherwood oil: methyl alcohol=5: 1: 1), separate white wax shape product 2.80g, yield 55%.
IR (v, cm
-1): 3427s (OH), 2885vs (Alkyl), 1597s (Ar), 1467s (Ar), 1176vs (CH
2-O-CH
2), 1112vs (CH
2-O-CH
2);
1H-NMR (500MHz, CDCl
3): δ=7.79-7.81 (m, 2H, arom), 7.33-7.35 (m, 2H, arom), 4.16 (t, J=6Hz, 2H, PhSO
2-OC
H 2 ), 3.49-3.79 (m, PEG oxygen methyl hydrogen), 2.45 (s, 3H, PhC
H 3 ).
The preparation of embodiment 5. compound V
2.15g IV and 0.11g sodiumazide are added to 25mL N, in the dinethylformamide, back flow reaction 5h.The pressure reducing and steaming solvent, residue washes (25mL * 2) with water with the dissolving of 30mL methylene dichloride, separates organic layer, anhydrous sodium sulfate drying filters, and concentrates, column chromatography (methylene dichloride: sherwood oil: methyl alcohol=5: 1: 1), separate light yellow wax shape product 1.30g, yield 65%.
IR(v，cm
-1)：3490s(-OH)，2868vs(Alkyl)，2103s(N
3)，1112vs(CH
2-O-CH
2)。
The preparation of embodiment 6. compound VI
2.0g compound V is dissolved in 10mL anhydrous methylene chloride/50mL dry toluene mixed solvent, adds 0.9g triphosgene and 0.1g N, accelerine, stirred overnight at room temperature.After reaction finished, reaction solution had been concentrated into a large amount of white precipitates and has generated, and leaves standstill 1h, filtered, and filter cake gets product crude product 1.9g, yield 95% with ice-cold anhydrous diethyl ether washed twice.
IR(v，cm
-1)：2886vs(Alkyl)，2102s(N
3)，1747s(O＝C)，1147vs(CH
2-O-CH
2)，1115vs(CH
2-O-CH
2)。
The preparation of embodiment 7. compound III
1.00g VI is joined in the 25mL anhydrous methylene chloride, dissolve colorless cleared solution, add 0.07g N then, accelerine and 0.38g DSPE, reaction solution reflux and to spend the night.Be cooled to room temperature then,, concentrate with 5% aqueous hydrochloric acid washing reaction liquid (15mL * 2), organic layer anhydrous sodium sulfate drying, column chromatography (methylene dichloride: sherwood oil: methyl alcohol=10: 1: 1), separate white wax 0.39g, yield 30%.
IR(v，cm
-1)：2889vs(Alkyl)，2106s(N
3)，1719s?(O＝C)，1280s(COO-C)，1148vs(CH
2-O-CH
2)，1115vs(CH
2-O-CH
2)。
Embodiment 8.NH
2The preparation of-PEG-DSPE
0.84g III is joined in 200mL ethanol/dichloromethane (1: the 1) mixed solvent, be heated to 50 ℃ and get colorless cleared solution, add 0.30g 10% palladium carbon then, under 10 normal atmosphere hydrogen, in 50 ℃ of reaction 4h.After reaction finishes, filtered while hot, filter cake hot ethanol washed twice.Boil off solvent, get light yellow solid 0.31g, yield 36%.
IR (v, cm
-1): 3403s (NH
2), 2917vs (Alkyl), 1738vs (O=C-O), 1641s (NH
2), 1250vs (COO-C), 1110vs (CH
2-O-CH
2), 557s (NH
2); MALDI-TOF mass-spectrometric data: 1715-2244 between molecular weight area;
1H-NMR (300MHz, CDCl
3): δ=4.00-3.40 (br, O-C
H 2 ), 2.30-2.50 (m, 2H, C
H 2 CO), and 1.62-0.89 (m, alkyl).
Claims (10)
1. the preparation method of a methods of distearoyl phosphatidyl ethanolamine is characterized in that this preparation method according to shown in the following reaction formula 1, carries out through step a, b, c three-step reaction; At first with phosphorus oxychloride and 1; the reaction of 2-distearyl glyceride obtains phosphinylidyne dichloro monoesters; abbreviate I as; again I and the effect of 2-hydroxyethylamino benzyl formate are obtained containing the DSPE of amino protecting group; letter is for being II; then the II catalytic hydrogenation is sloughed protecting group and obtain methods of distearoyl phosphatidyl ethanolamine, abbreviate DSPE as
Reaction formula 1.
2. the preparation method of methods of distearoyl phosphatidyl ethanolamine according to claim 1, it is characterized in that a step reaction in the reaction formula 1 is under nitrogen protection, alkali is dissolved in the anhydrous aprotic solvent, under 0 ℃, drip phosphorus oxychloride and 1 respectively, the solution of the above-mentioned solvent of 2-distearyl glyceride, 0 ℃ of reaction, gained I reaction solution is directly used in b step reaction without separation then; Wherein, alkali is tertiary amine, triethylamine, Tributylamine, pyridine or N, accelerine, preferred triethylamine; Aprotic solvent is chloroform, methylene dichloride, N, dinethylformamide or tetrahydrofuran (THF), preferred tetrahydrofuran (THF); Phosphorus oxychloride, 1, the molar ratio of 2-distearyl glyceride and alkali are 0.9-1.3: 1: 1, preferred proportion was 1.2: 1: 1.
3. the preparation method of methods of distearoyl phosphatidyl ethanolamine according to claim 1, it is characterized in that in the b step reaction in the reaction formula 1, reaction solution with gained I in a step reaction, under nitrogen protection, below 10 ℃, drip the solution of the anhydrous aprotic solvent of 2-hydroxyethylamino benzyl formate and alkali, in room temperature reaction, add water then and stop, obtain II through column chromatography for separation; Wherein, alkali is tertiary amine, triethylamine, Tributylamine, pyridine or N, accelerine, preferred triethylamine; Aprotic solvent is chloroform, methylene dichloride, N, dinethylformamide or tetrahydrofuran (THF), preferred tetrahydrofuran (THF); The molar ratio of 2-hydroxyethylamino benzyl formate, alkali and I is 1: 1: 1; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride, and alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
4. the preparation method of methods of distearoyl phosphatidyl ethanolamine according to claim 1, it is characterized in that in the c step reaction in the described reaction formula 1, gained II in the described b step reaction is dissolved in alkyl alcohol and the methyl chloride mixed solvent, add catalyzer, carbobenzoxy-(Cbz) is sloughed in hydrogenation, obtains product D SPE; Wherein, alkyl alcohol is methyl alcohol or ethanol, and methyl chloride is methylene dichloride or chloroform, the mixed solvent that preferred alcohol and methylene dichloride are formed, and the ratio of alkyl alcohol and methyl chloride is at 1-3 in the mixed solvent: between 1, preferred proportion is 1: 1; Catalyzer is palladium carbon or Raney nickel, preferred 10% palladium carbon.
5. the preparation method of the amino polyethylene glycol derivatives of a methods of distearoyl phosphatidyl ethanolamine is characterized in that this preparation method according to shown in the following reaction formula 2, carries out through steps d and e two-step reaction; At first with the PEG chloro-formic ester of DSPE and nitrine replacement, abbreviate VI as, reaction obtains the DSPE derivative of azido-Pegylation, abbreviate III as, by catalytic hydrogenation azido-is reduced to amino then, obtains the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine, abbreviate NH as
2-PEG-DSPE,
Reaction formula 2.
6. the preparation method of the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine according to claim 5, it is characterized in that in the presence of alkali, VI being dissolved in the anhydrous methyl chloride in the d step reaction in the described reaction formula 2, with equimolar DSPE reaction, obtain III through column chromatography for separation; Wherein, alkali is tertiary amine, triethylamine, Tributylamine, pyridine or N, accelerine, preferred N, accelerine; The methyl chloride solvent is chloroform or methylene dichloride, preferred methylene dichloride; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride, and alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
7. the preparation method of the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine according to claim 5, it is characterized in that in the e step reaction in the described reaction formula 2, gained III in the d step reaction is dissolved in alkyl alcohol and the methyl chloride mixed solvent, add catalyzer hydrogenation, obtain NH
2-PEG-DSPE; Wherein, alkyl alcohol is methyl alcohol or ethanol, and methyl chloride is methylene dichloride or chloroform, the mixed solvent that preferred alcohol and methylene dichloride are formed, and the ratio of alkyl alcohol and methyl chloride is at 1-3 in the mixed solvent: between 1, preferred proportion is 1: 1; Catalyzer is palladium carbon or Raney nickel, preferred 10% palladium carbon.
8. the preparation method of the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine according to claim 5 is characterized in that PEG chloro-formic ester that described nitrine replaces according to shown in the following reaction formula 3, carries out through step f and g two-step reaction; At first, abbreviate IV as, carry out substitution reaction with sodiumazide and obtain the PEG that single azido-replaces, abbreviate V as, then it and triphosgene reaction are obtained the PEG chloro-formic ester that nitrine replaces, abbreviate VI as PEG-tosic acid monoesters,
Reaction formula 3.
9. the preparation method of the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine according to claim 8, it is characterized in that in the f step reaction in the described reaction formula 3, IV and sodiumazide are dissolved in N, react in the dinethylformamide, obtain V through column chromatography for separation; Wherein, the molar ratio of IV and sodiumazide is 1: 1-3, preferred proportion are 1: 2; The used eluent of column chromatography for separation is the mixed solvent of being made up of methyl chloride, sherwood oil and alkyl alcohol, and methyl chloride is methylene dichloride or chloroform, preferred methylene dichloride, and alkyl alcohol is methyl alcohol, ethanol or Virahol, particular methanol.
10. the preparation method of the amino polyethylene glycol derivatives of methods of distearoyl phosphatidyl ethanolamine according to claim 8, it is characterized in that in the g step reaction in the described reaction formula 3, in the presence of alkali, V is dissolved in the mixed solvent of anhydrous methyl chloride and anhydrous alkylbenzene, with the triphosgene effect, obtain VI; Wherein, alkali is tertiary amine, triethylamine, Tributylamine, pyridine or N, accelerine, preferred N, accelerine; The molar ratio of V, alkali and triphosgene is 1: 1: 1-3, preferred proportion are 1: 1: 3; The methyl chloride solvent is methylene dichloride or chloroform, preferred methylene dichloride, and alkyl benzene solvent is toluene, ethylbenzene or dimethylbenzene, preferred toluene.
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| CN102580608A (en) * | 2012-01-12 | 2012-07-18 | 浙江大学 | Amphiphilic small molecular surfactant based on cholesterol and phosphorylcholine and preparation method thereof |
| CN108624381A (en) * | 2018-06-07 | 2018-10-09 | 中国林业科学研究院林产化学工业研究所 | A kind of water base lubricant and preparation method thereof |
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| CN112552336A (en) * | 2020-12-10 | 2021-03-26 | 深圳先进技术研究院 | Preparation method of distearoyl phosphatidyl ethanolamine |
| CN113831265A (en) * | 2020-06-23 | 2021-12-24 | 中国科学技术大学 | Hydroxyl compound end modified functional group and method for modifying hydroxyl compound by using same |
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| CN102580608A (en) * | 2012-01-12 | 2012-07-18 | 浙江大学 | Amphiphilic small molecular surfactant based on cholesterol and phosphorylcholine and preparation method thereof |
| CN102580608B (en) * | 2012-01-12 | 2014-01-29 | 浙江大学 | Amphiphilic small molecular surfactant based on cholesterol and phosphorylcholine and preparation method thereof |
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| CN108624381A (en) * | 2018-06-07 | 2018-10-09 | 中国林业科学研究院林产化学工业研究所 | A kind of water base lubricant and preparation method thereof |
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| CN112552336A (en) * | 2020-12-10 | 2021-03-26 | 深圳先进技术研究院 | Preparation method of distearoyl phosphatidyl ethanolamine |
| WO2022120689A1 (en) * | 2020-12-10 | 2022-06-16 | 深圳先进技术研究院 | Preparation method for distearoyl phosphatidylethanolamine |
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