CN101787345B - Device for collecting and separating body fluid sample used for cell examination and method therefor - Google Patents
Device for collecting and separating body fluid sample used for cell examination and method therefor Download PDFInfo
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- CN101787345B CN101787345B CN 201010120630 CN201010120630A CN101787345B CN 101787345 B CN101787345 B CN 101787345B CN 201010120630 CN201010120630 CN 201010120630 CN 201010120630 A CN201010120630 A CN 201010120630A CN 101787345 B CN101787345 B CN 101787345B
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Abstract
The invention relates to a device for collecting and separating a body fluid sample used for cell examination and a method therefor. The device comprises a sample collection container and a cell smear device, wherein the sample collection container comprises a container body with an opening at the top part; a lateral liquid discharge hole provided with a liquid discharge hole switch is arranged at the lower part of the side wall of the container body; the bottom of the container body is of the V shape or the turbination; and a T-shaped collection pipe is arranged at the bottom of the V shape or the turbination. The method comprises: adding various body fluid samples into the container body for resting, so that the visible components such as cells and the like in the body fluid samples can be naturally precipitated into the collection pipe, and the liquid supernatant of the body fluid can be discharged by the liquid discharge hole; and then taking down the collection pipe and directly fixing the collection pipe on the cell smear device, and adopting a general centrifugation smear machine to prepare a cell smear directly. The device and the method can successfully collect large amount of the visible components such as cells, sediment and the like in the body fluid sample, can achieve the aim of obviously improving the relevance ratio of positive cells and can be widely used for the cytological examination of various body fluid samples.
Description
Technical field
The invention belongs to cytolgical examination utensil in the medicine equipment of medical field, particularly be used for body fluid sample collection, tripping device and the method for cytoscopy in a kind of available clinical body fluid sample (hydrothorax, ascites, urine, cerebrospinal fluid, drainage-fluid) and cell culture fluid, the inoculum.
Background technology
Carrying out cytolgical examination for body fluid samples such as hydrothorax, ascites, urine, cerebrospinal fluid, various body cavity drainage-fluids is daily curative activity, particularly the important component part of cell pathology worker routine work.Can effectively collect, separate quality, cytopathologic diagnosis and positive rate that the formed elements in the above-mentioned body fluid sample, particularly various cast-off cells are directly connected to cell smear, determine that also can the patient be made a definite diagnosis timely and effectively treatment.Can the formed elements that therefore, effectively collect, separate in the body fluid samples such as hydrothorax, ascites, urine, cerebrospinal fluid, various body cavity drainage-fluids be the important prerequisite that guarantees this work.
But, in daily medical practice, general various body cavity drainage-fluid is usually more, hydrothorax particularly, ascites, the body fluid samples such as urine are bulky especially, frequent hundreds of milliliter at least, thousands of milliliters at most, therefore, if just extracting a small amount of sample carries out cytolgical examination and has undoubtedly the huge risk of failing to pinpoint a disease in diagnosis, but, owing to lack at present suitable utensil, therefore, will be to all body fluid samples (such as hundreds of milliliters hydrothorax, ascites, or thousands of milliliters twenty-four-hour urine fluid samples) cast-off cells collects in, separation be one loaded down with trivial details and be difficult to obtain the treatment process of promising result, generally at present adopt following method: (1) is used and is outwelled supernatant after larger container (such as infusion bottle) leaves standstill, and the liquid smear of drawing again container bottom carries out cytolgical examination; (2) adopt large capacity centrifuge tube to above-mentioned body fluid sample gradation, carry out centrifugation in batches, be collected in one, then smear carries out cytolgical examination; Obviously, aforesaid method is very loaded down with trivial details, but also might because the part cell adheres to still to cause on the tube wall and fails to pinpoint a disease in diagnosis, also have simultaneously the risk of polluting.
As seen, adopt existing method that body fluid sample is carried out loaded down with trivial details, the easy pollution of cytolgical examination work, efficient shortcoming low, easy to cause missed diagnosis.
Equally, also there are the problems referred to above for cell, bacterium in the cell cultures in life science field, the microbial culture in separating.
Therefore, adapt to the clinical and cytopathology need of work of actual medical, research and development can improve body fluid sample collection, the tripping device using method of body fluid sample positive rate, have important economic benefit and social benefit.
Summary of the invention
The object of the invention is to defective or deficiency for above-mentioned prior art existence, provide a kind of body fluid sample for cytoscopy to collect, tripping device and method, be used for collecting, the formed elements such as cell provides basic basis to cytopathology inspection and research in the various body fluid thereby separate, and provide a kind of use said apparatus quick collection, the method of separating the formed elements such as cell in the various body fluid samples, the method can overcome now methodical defective, particularly overcomes in the situation that the body fluid sample amount reaches hundreds if not thousands of milliliters to collect, the shortcoming that prior art exists during cast-off cells in the separating sample.
For achieving the above object, the inventive system comprises sample collection container and cell smearing device, said sample collection container comprises that the top is provided with the container body of opening, lower sidewall at container body is provided with one with the horizontal outage of outage switch, the bottom of container body is " V " font or turbination, is provided with "T"-shaped collection tube at " V " font or turbination bottom.
Container body of the present invention and "T"-shaped collection tube adopt the standby connection type bag-like container of integration system, and at opening filling orifice are installed, and container body top is provided with for keeping the stable container suspender belt hole of container.
Container body of the present invention is bottle or tank body, "T"-shaped collection tube adopts dismountable mode to be arranged on the bottom of container body, comprise the identical pickup groove with common slide glass of a size with the cell smearing device that "T"-shaped collection tube matches, be provided be used to the aperture of discharging unnecessary liquid and unloading slide glass in the pickup groove bottom, pickup groove upper limb both sides are respectively arranged with the retaining clip that is used for the retaining clip open holes of U-shaped retaining clip being installed and being locked retaining clip of two symmetries and link up with, be disposed with slide glass and pad above the pickup groove, the pad center offers a through hole identical with "T"-shaped collection tube openings of sizes, and cell smearing device is installed on the "T"-shaped collection tube by the U-shaped retaining clip; Be provided with the lid with filling orifice on the opening at container body top; "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube tube wall and with "T"-shaped collection tube mouth of pipe place and same plane, size is identical with slide glass or be slightly less than the collection tube retaining plate of slide glass; The bottom of container body is provided for keeping the stable pan straddle of container or container top that container suspender belt hole is set.
The body fluid sample collection, tripping device and the method that are used for cytoscopy, comprise the following steps: 1) select the container body of suitable size according to the amount of pending body fluid, make the container body horizontal positioned by pan straddle or container suspender belt hole, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body from the upper end, perhaps be connected with drainage tube, directly collect body fluid; 2) body fluid leaves standstill more than the 20min after joining container body, so that the various formed elements natural sediments such as cell in the above-mentioned body fluid sample are in the "T"-shaped collection tube of container body bottom " V " font or turbination bottom; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube after, open the outage switch of container body sidewall the body fluid supernatant discharged by outage; 4) if the body fluid sample amount is large, surpass humor collecting container primary treatment ability, then repeatedly carry out step 1)～3) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube smoothly; 5) after all body fluid sample was finished dealing with, the tilt container body was poured out remaining body fluid supernatant; If container body and "T"-shaped collection tube adopt the standby connection type bag-like container of integration system, then directly cut "T"-shaped collection tube with scissors, re-use common cell smear and prepare apparatus and prepare cell smear; If container body is bottle or tank body, then take off "T"-shaped collection tube, add successively Upper gasket, slide glass and pickup groove at its top, utilize the U-shaped retaining clip pad, slide glass and pickup groove to be fixed on the retaining plate at "T"-shaped collection tube mouth of pipe position, then put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear.
The present invention uses the bottom to be the collection container body fluid sample of " V " font or inversed-cone shape, utilize action of gravity, make various formed elements natural subsidence such as cell in the body fluid sample, be slipped to the collection tube that is positioned at the minimum position of container, discharge the body fluid sample supernatant liquor by the outage that is positioned at the bottle side wall bottom, reach the purpose of the formed elementss such as cast-off cells in smooth collection, the chorista fluid samples.Simultaneously, by using the cell smearing device that is complementary with existing centrifugal smearing machine rotor, can directly the formed elementss such as cast-off cells in the collection tube evenly be coated on the slide glass preparation cell smear.
If the collection tube of collection container bottom and bottle (or tank body or bag) are for adopting the connection type structure of integrated design preparation, then directly adopt scissors to cut collection tube, use centrifugal smearing machine to carry out further separation and the film-making of formed elements in the body fluid sample.
Certainly, these apparatus and method also can be used for separating and the smear preparation of cell (bacterium) in cell cultures and the inoculum.
Description of drawings
Fig. 1 is the structural representation of the sample collection container of the embodiment of the invention 1 integrated design; Fig. 2 is the structural representation of the sample collection container of the embodiment of the invention 2; Fig. 3 is the structural representation of the embodiment of the invention 2 cell smearing devices; Fig. 4 is the scheme of installation of the embodiment of the invention 2 "T"-shaped collection tubes and cell smearing device; Fig. 5 is the structural representation after the embodiment of the invention 2 "T"-shaped collection tubes and cell smearing device are installed; Wherein: 1 container body 1,2 openings, 3 lids, 4 filling orifices, 5 outages, 6 outage switches, 7 "T"-shaped collection tubes, 8 collection tube retaining plates, 9 pan straddles, 10 container suspender belt holes, 11 pickup grooves, 12 apertures, 13,14 retaining clips hook, 15 retaining clips, 16 pads, 17 slide glasss.
Embodiment
The present invention is described in further detail according to specific embodiment that technical scheme of the present invention is finished below in conjunction with accompanying drawing and contriver.
Referring to Fig. 1～5, Fig. 1～5 also are specific embodiments of the invention, but the invention is not restricted to this embodiment.
Embodiment 1: referring to Fig. 1, the present invention includes sample collection container and cell smearing device, said sample collection container comprises that the top is provided with the container body 1 of opening 2, lower sidewall at container body 1 is provided with one with the horizontal outage 5 of outage switch 6, the bottom of container body 1 is " V " font or turbination, be provided with "T"-shaped collection tube 7 at " V " font or turbination bottom, wherein container body 1 adopts the standby connection type bag-like container of integration system with "T"-shaped collection tube 7, and at opening 2 filling orifice 4 is installed, container body 1 top is provided with for keeping the stable container suspender belt hole 10 of container.
The body fluid sample of its cytoscopy is collected, separation method is: the container body 1 of 1) selecting suitable size according to the amount of pending body fluid, make container body 1 horizontal positioned by container suspender belt hole 10, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and the inoculum filling orifice 4 from container body 1 upper end is joined in the container body 1, perhaps be connected with drainage tube, directly collect body fluid; 2) body fluid leaves standstill more than the 20min after joining container body 1, so that the various formed elements natural sediments such as cell in the above-mentioned body fluid sample are in the "T"-shaped collection tube 7 of container body 1 bottom " V " font or turbination bottom; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube 7 after, open the outage switch 6 of container body 1 sidewall the body fluid supernatant discharged by outage 5; 4) if the body fluid sample amount is large, surpass humor collecting container primary treatment ability, then repeatedly carry out step 1)～3) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube 7 smoothly; 5) after all body fluid samples were finished dealing with, tilt container body 1 was poured out remaining body fluid supernatant, directly cuts "T"-shaped collection tube 7, re-used common cell smear and prepared apparatus and prepare cell smear; Above-mentioned "T"-shaped collection tube 7 also can be used as centrifuge tube, uses generic centrifuge to carry out the further separation of formed elements in the body fluid sample.If the formed elements quantity such as the interior cast-off cells of "T"-shaped collection tube are more, can save backup, also can draw the part cellular constituent as different purposes with pipettor according to the real work needs.
Embodiment 2: referring to Fig. 2～5, the present invention includes sample collection container and cell smearing device, said sample collection container comprises that the top is provided with the bottle of opening 2 or the container body 1 that tank body is made, be provided with the lid 3 with filling orifice 4 on the opening 2 at container body 1 top, the bottom of container body 1 is provided for keeping the stable pan straddle of container 9 or container top that container suspender belt hole 10 is set, the lower sidewall of container body 1 is provided with one with the horizontal outage 5 of outage switch 6, the bottom of container body 1 is " V " font or turbination, be provided with dismountable "T"-shaped collection tube 7 at " V " font or turbination bottom, said cell smearing device comprises a size and common slide glass 17 identical pickup grooves 11, be provided be used to the aperture 12 of discharging unnecessary liquid and unloading slide glass 17 in pickup groove 11 bottoms, pickup groove 11 upper limb both sides are respectively arranged with the retaining clip that is used for the retaining clip open holes 13 of U-shaped retaining clip 15 being installed and being locked retaining clip 15 of two symmetries and link up with 14, be disposed with slide glass 17 and pad 16 above the pickup groove 11, pad 16 centers offer a through hole identical with "T"-shaped collection tube 7 openings of sizes, said "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube 7 tube walls and with "T"-shaped collection tube 7 mouth of pipe place and same planes, size is identical with slide glass 17 or be slightly less than the collection tube retaining plate 8 of slide glass 17, and cell smearing device is installed on the collection tube retaining plate 8 of "T"-shaped collection tube 7 outers by U-shaped retaining clip 15.
The body fluid sample of its cytoscopy is collected, separation method, comprise the following steps: 1) select the container body 1 of suitable size according to the amount of pending body fluid, make container body 1 horizontal positioned by pan straddle 9 or container suspender belt hole 10, directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body 1 from the upper end, perhaps be connected with drainage tube, directly collect body fluid; 2) body fluid leaves standstill more than the 20min after joining container body 1, so that the various formed elements natural sediments such as cell in the above-mentioned body fluid sample are in the "T"-shaped collection tube 7 of container body 1 bottom " V " font or turbination bottom; 3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube 7 after, open the outage switch 6 of container body 1 sidewall the body fluid supernatant discharged by outage 5; 4) if the body fluid sample amount is large, surpass humor collecting container primary treatment ability, then repeatedly carry out step 1)～3) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube 7 smoothly; 5) after all body fluid sample is finished dealing with, tilt container body 1 is poured out remaining body fluid supernatant, container body 1 is bottle or tank body, then take off "T"-shaped collection tube 7, add successively Upper gasket 16, slide glass 17 and pickup groove 11 at its top, utilize U-shaped retaining clip 15 that pad 16, slide glass 17 and pickup groove 11 are fixed on the retaining plate 8 at "T"-shaped collection tube 7 mouth of pipe positions, then put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear.
According to design and the method for the invention described above, the device of the application of the invention can be very convenient be collected, the formed elements such as the cell in the chorista fluid samples and prepare cell smear fast.Certainly, these apparatus and method also can be used for separating and the smear preparation of cell (bacterium) in cell cultures and the inoculum.
Claims (7)
- A body fluid sample that is used for cytoscopy collect, tripping device, comprise sample collection container and cell smearing device, it is characterized in that: said sample collection container comprises that the top is provided with the container body of opening (2) (1), lower sidewall at container body (1) is provided with one with the horizontal outage (5) of outage switch (6), the bottom of container body (1) is " V " font or turbination, is provided with "T"-shaped collection tube (7) at " V " font or turbination bottom.
- Body fluid sample for cytoscopy according to claim 1 collect, tripping device, it is characterized in that: said container body (1) adopts the standby connection type bag-like container of integration system with "T"-shaped collection tube (7), and at opening (2) filling orifice (4) is installed, container body (1) top is provided with for keeping the stable container suspender belt hole (10) of container.
- 3. the body fluid sample for cytoscopy according to claim 1 is collected, tripping device, it is characterized in that: said container body (1) is bottle or tank body, "T"-shaped collection tube (7) adopts dismountable mode to be arranged on the bottom of container body (1), comprise a size and the identical pickup groove of common slide glass (17) (11) with the cell smearing device that "T"-shaped collection tube (7) matches, be provided be used to the aperture (12) of discharging unnecessary liquid and unloading slide glass (17) in pickup groove (11) bottom, pickup groove (11) upper limb both sides are respectively arranged with the retaining clip that is used for the retaining clip open holes (13) of U-shaped retaining clip (15) being installed and being locked retaining clip (15) of two symmetries and link up with (14), be disposed with slide glass (17) and pad (16) above the pickup groove (11), pad (16) center offers a through hole identical with "T"-shaped collection tube (7) openings of sizes, and cell smearing device is installed on the "T"-shaped collection tube (7) by U-shaped retaining clip (15).
- 4. body fluid sample collection, tripping device for cytoscopy according to claim 3 is characterized in that: be provided with the lid (3) with filling orifice (4) on the opening (2) at said container body (1) top.
- Body fluid sample for cytoscopy according to claim 3 collect, tripping device, it is characterized in that: said "T"-shaped collection tube peristome be provided with one vertical with "T"-shaped collection tube (7) tube wall and with "T"-shaped collection tube (7) mouth of pipe place and same plane, big or small identical with slide glass (17) or be slightly less than the collection tube retaining plate (8) of slide glass (17).
- Body fluid sample for cytoscopy according to claim 3 collect, tripping device, it is characterized in that: pan straddle (9) or container top that the bottom of said container body (1) is provided for keeping container stable arrange container suspender belt hole (10).
- 7. a use is carried out the method for collecting, separating for the body fluid sample of cytoscopy such as device as described in claim 2 or 3, it is characterized in that, comprises the following steps:1) selects the container body (1) of suitable size according to the amount of pending body fluid, make container body (1) horizontal positioned by pan straddle (9) or container suspender belt hole (10), directly hydrothorax, ascites, urine, cerebrospinal fluid, draining fluids and cell culture fluid and inoculum are joined in the container body (1) from the upper end, perhaps be connected with drainage tube, directly collect body fluid;2) body fluid leaves standstill more than the 20min after joining container body (1), so that the various formed elements natural sediments such as cell in the above-mentioned body fluid sample are in the "T"-shaped collection tube (7) of container body (1) bottom " V " font or turbination bottom;3) treat that various formed elements natural subsidence such as cell in the sample are slipped in the "T"-shaped collection tube (7) after, open the outage switch (6) of container body (1) sidewall the body fluid supernatant discharged by outage (5);4) if the body fluid sample amount is large, surpass humor collecting container primary treatment ability, then repeatedly carry out step 1)～3) method collects the formed elementss such as cell in the sample in the "T"-shaped collection tube (7) smoothly;5) after all body fluid sample was finished dealing with, tilt container body (1) was poured out remaining body fluid supernatant;If container body (1) and "T"-shaped collection tube (7) adopt the standby connection type bag-like container of integration system, then directly cut "T"-shaped collection tube (7) with scissors, re-use common cell smear and prepare apparatus and prepare cell smear;If container body (1) is bottle or tank body, then take off "T"-shaped collection tube (7), add successively Upper gasket (16), slide glass (17) and pickup groove (11) at its top, utilize U-shaped retaining clip (15) pad (16), slide glass (17) and pickup groove (11) to be fixed on the retaining plate (8) at "T"-shaped collection tube (7) mouth of pipe position, then put it in the rotor or rotary head hanging basket of cell centrifugation smearing machine direct centrifugal preparation cell smear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010120630 CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010120630 CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
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| Publication Number | Publication Date |
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| CN101787345A CN101787345A (en) | 2010-07-28 |
| CN101787345B true CN101787345B (en) | 2013-04-17 |
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| CN 201010120630 Expired - Fee Related CN101787345B (en) | 2010-03-10 | 2010-03-10 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN114018667A (en) * | 2015-09-14 | 2022-02-08 | 国立大学法人滋贺医科大学 | Cell-retaining substrate holder for producing observation specimen, kit including same, and method for producing observation specimen |
| CN109022281A (en) * | 2018-08-16 | 2018-12-18 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel cell culture vessel |
| TWI661044B (en) * | 2018-10-19 | 2019-06-01 | 薩摩亞商Ｓｃｌ生物科技有限公司 | Culture bottle and culture bottle assembly |
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|---|---|---|---|---|
| DE3321886C1 (en) * | 1983-06-16 | 1984-05-24 | Dieter Prof. Dr.-Ing. 7500 Karlsruhe Wurz | Method and apparatus for obtaining samples of, in particular, microbial constituents in gas flows |
| CN1503910A (en) * | 2000-11-08 | 2004-06-09 | ���˶ٵϿ�ɭ��˾ | Method and device for collecting and stabilizing biological sample |
| CN201000400Y (en) * | 2006-12-30 | 2008-01-02 | 杭州晶格科学仪器有限公司 | Diaphragm type liquid foot cell pellet mill |
| CN101511991A (en) * | 2006-09-04 | 2009-08-19 | 贝尔坦技术有限公司 | Device for collecting and separating particles and microorganisms present in the ambient air |
| CN201372284Y (en) * | 2009-03-14 | 2009-12-30 | 刘善利 | Blood specimen collection and culture bottle |
-
2010
- 2010-03-10 CN CN 201010120630 patent/CN101787345B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3321886C1 (en) * | 1983-06-16 | 1984-05-24 | Dieter Prof. Dr.-Ing. 7500 Karlsruhe Wurz | Method and apparatus for obtaining samples of, in particular, microbial constituents in gas flows |
| CN1503910A (en) * | 2000-11-08 | 2004-06-09 | ���˶ٵϿ�ɭ��˾ | Method and device for collecting and stabilizing biological sample |
| CN101511991A (en) * | 2006-09-04 | 2009-08-19 | 贝尔坦技术有限公司 | Device for collecting and separating particles and microorganisms present in the ambient air |
| CN201000400Y (en) * | 2006-12-30 | 2008-01-02 | 杭州晶格科学仪器有限公司 | Diaphragm type liquid foot cell pellet mill |
| CN201372284Y (en) * | 2009-03-14 | 2009-12-30 | 刘善利 | Blood specimen collection and culture bottle |
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