CN101775358B - Accharomyces cerevisiae and application thereof in adsorption of aflatoxin - Google Patents
Accharomyces cerevisiae and application thereof in adsorption of aflatoxin Download PDFInfo
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Abstract
The invention discloses accharomyces cerevisiae and an application thereof in adsorption of aflatoxin B1. The bacterial strain is accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382. After being fermented, the bacterial strain produces thallus which has the adsorption on the aflatoxin B1. The test of the adsorption of accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382 on the aflatoxin B1 in a culture medium shows that the adsorption rate of the thallus of the accharomyces cerevisiae on the aflatoxin B1 reaches 81.16%; and the test of the adsorption of accharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1GGMCCNo 3382 on the aflatoxin B1 in a peanut butter shows that the adsorption rate of the thallus of the accharomyces cerevisiae on the aflatoxin B1 in the peanut butter reaches 88.32%.
Description
Technical field
The present invention relates to an Accharomyces cerevisiae and application thereof, particularly relate to yeast saccharomyces cerevisiae and the application in adsorption of aflatoxin thereof.
Background technology
Toxins, afla (Aflatoxin AFT) is flavus (A.flavus), the mould mycetogenetic secondary metabolites such as (A.nomius) in Aspergillus parasiticus (A.parasiticus) and Tequ, and common have an AFB
1, AFB
2, AFG
1, AFG
2, AFM
1, AFM
2, AFB
2a, AFG
2a, AFBM
2aAnd AFGM
2aDeng, AFB wherein
1(AFB
1) toxicity the strongest.A large amount of epidemiology surveys and research all confirm AFB
1Can bring out the foundation of human liver cell JEG-3 with hepatitis B virus synergy.AFB
1Also can influence animal embryo, cause immunosuppression and repeated infection, reduce giving milk and egg productivity of some animal.Toxins, afla mainly is present in corn, in the foods such as peanut, has a strong impact on the outlet of China's peanut and goods thereof; In addition, feed also often receives the pollution of Toxins, afla.Therefore, the poison-removing method that presses for a kind of highly effective and safe is eliminated the harm of this toxoid to human and livestock health.
At present, the biology poison-removing method of Toxins, afla mainly contains following several kinds:
1, the enzyme liberating Toxins, afla that produces of microbial metabolism: for example from Nocardia bacteria DSM 12676, have a liking for the degrading enzyme that extracts the narrow food Zymomonas mobilis of wheat, Armillariella tabescens, mycobacterium DSM 44556T, Rhodococcus and the oyster cap fungus; This method degradation rate is higher; Condition effect such as but enzyme is subject to pH, temperature and inactivation, in addition; The also security of not research proof Toxins, afla degraded product at present is so method possibly produce secondary pollution.
2, microbial cells itself and cell wall extracts adsorption of aflatoxin thereof: for example lactobacillus rhamnosus GG, lactobacillus rhamnosus LC-705, lactobacterium casei cheese subspecies CGMCC 1.539 and some yeast saccharomyces cerevisiae; Above-mentioned bacterial strains can combine with Toxins, afla through physics mode; Form toxin-thalline mixture; This type of mixture is prone to and food separation, can reach to remove AFB in the food
1Purpose.This method is easy, economical, can not produce secondary pollution, has development prospect preferably.
Summary of the invention
The purpose of this invention is to provide an Accharomyces cerevisiae, this bacterial strain thalline itself can adsorption of aflatoxin, and this thalline can be used for preparing the biotechnological formulation of adsorption of aflatoxin, is used for the biological detoxication of Toxins, afla.
Yeast saccharomyces cerevisiae provided by the present invention is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1.
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 2nd, 2009 and (is called for short CGMCC; The address is: the Datun Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica), preservation registration number is: CGMCC № 3382.
S. cervisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is that dough from traditional spontaneous fermentation of being used for making steamed bun separates and obtains.
Another object of the present invention provides a kind of adsorption of aflatoxin B
1Biotechnological formulation.
Adsorption of aflatoxin B provided by the present invention
1Biotechnological formulation, be the thalline that ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 obtains.
Said thalline is to obtain after the centrifugal removal supernatant of fermented liquid with yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
In the above-mentioned biotechnological formulation, the fermention medium of said ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 consists of: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water; Said percentage composition is the quality percentage composition;
The pH value of said fermention medium is 7.0-7.2.
Said fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, shaking table rotation radius 20mm～26mm, shaking culture 30h～34h.
The thalline of said acquisition is also through 121 ℃ of heating.The step of said heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer (pH6.0) again at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline.
The 3rd purpose of the present invention provides above-mentioned adsorption of aflatoxin B
1The preparation method of biotechnological formulation.
Adsorption of aflatoxin B provided by the present invention
1The preparation method of biotechnological formulation, be ferment wine brewing yeast S.cerevisiae) LYMT-Y1 CGMCC № 3382, from its fermented liquid, separate the thalline that obtains and be adsorption of aflatoxin B
1Biotechnological formulation.
Among the above-mentioned preparation method, the fermention medium of said ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382 consists of: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water; Said percentage composition is the quality percentage composition;
The pH value of said fermention medium is 7.0-7.2.
Said fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, shaking table rotation radius 20mm～26mm, shaking culture 30h～34h.
The thalline of said acquisition is also through 121 ℃ of heating.The step of said heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer (pH6.0) again at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline.
Ferment wine brewing yeast of the present invention (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 and above-mentioned biotechnological formulation are at adsorption of aflatoxin B
1In application also belong to protection scope of the present invention.
In the said application, said biotechnological formulation or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382 adsorption of aflatoxin B
1Temperature condition be 20-30 ℃, be preferably 23 ℃; The time of said absorption is 30-120min, preferred 30min.In order to reach the good adsorption effect, as required, the addition of said yeast saccharomyces cerevisiae (S.cerevisiae) LYMT-Y1 CGMCC № 3382 can be 2.5 * 10
9Individual yeast cell/ml treats more than the absorption system, in order to save cost, raises the efficiency, and the addition of said yeast saccharomyces cerevisiae can be 2.5 * 10
9-1.7 * 10
10Individual yeast cell/ml treats absorption system.
Experiment showed, 3382 pairs of AFBs of yeast saccharomyces cerevisiae of the present invention (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Has very strong adsorptive power.AFB in yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) the LYMT-Y1 absorption substratum
1Experiment show, when the thalline addition is 1.7 * 10
10Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 30 ℃, and adsorption time is 2h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 94.32%, AFB
1Concentration be reduced to 1.31ng/g from 23.11ng/g; When the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 25 ℃, and adsorption time is 1h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 81.16%, AFB
1Concentration be reduced to 3.76ng/g from 19.95ng/g.AFB in yeast saccharomyces cerevisiae (S.cerevisiae) the LYMT-Y1 absorption peanut paste
1Experiment show that the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 23 ℃, and adsorption time is 30min, and shaking speed is 200rpm, and the thalline of yeast saccharomyces cerevisiae is to AFB in the peanut paste
1Adsorption rate reach 88.32%, AFB in the peanut paste
1Concentration be reduced to 2.63ng/g from 22.50ng/g.
Therefore, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 and with its preparation biotechnological formulation at AFB
1Removal in have a good application prospect.
Description of drawings
Fig. 1 is the concrete form of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
Fig. 2 is the flat-plate bacterial colony form of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1.
Embodiment
Experimental technique described in the following embodiment like no specified otherwise, is ordinary method; Said reagent and biomaterial like no specified otherwise, all can obtain from commercial sources.
Realize that the substratum that the present invention adopted is specific as follows said:
Yeast (YPD) substratum: yeast soaks powder 1%, peptone 2%, and glucose 2%, all the other are water, said percentage composition is the quality percentage composition.The agar that in the YPD liquid nutrient medium, adds 1.5% quality percentage composition promptly obtains the YPD solid medium.
Separation, purifying and the evaluation of embodiment 1, yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382.
In March, 2009, in Bechtop, to take and be placed in traditional spontaneous fermentation dough of making steamed bun that vibration 15min prepares bacteria suspension in the sterile distilled water, shaking speed is 180rpm; Bacteria suspension is coated on the YPD culture medium flat plate after with the sterilized water doubling dilution, cultivates under 25 ℃ of conditions, behind about 40h, bacterium colony is covered with whole flat board, places the YPD liquid nutrient medium with several the different bacterium colonies on the transfering loop picking flat board, and 30h is cultivated in concussion.The thalline that then centrifugal above-mentioned fermented liquid is obtained is applied to AFB
1Adsorption test, thus filter out AFB
1The bacterial strain that adsorptive power is the strongest.Dyed back is observed under optical electron microscope and the Physiology and biochemistry experiment confirms that this bacterial strain is a yeast saccharomyces cerevisiae.The above-mentioned bacterial strain of confirming as yeast saccharomyces cerevisiae is carried out enlarged culturing and with its called after LYMT-Y1.
According to saccharomycetic characteristic and identification handbook (English Barnett, work such as J.A.; Hu Ruiqing translates, press of Qingdao Marine University, 1991.6) the middle method of describing, bacterial strain LYMT-Y1 CGMCC № 3382 is carried out morphological specificity, cultural characters and physio-biochemical characteristics identify that concrete outcome is following:
(1) morphological specificity of thalline
This strain cell ovalize, gemmation, each cell produces one or more gemma, and gemma is ovalization also.The concrete form of this strain cell is seen Fig. 1.
(2) cultural characters
Bacterium colony is creamy white, smooth, circular, neat in edge, colony diameter 3-4mm, aerobic, bacterium colony is soft and moistening, the even picking easily of quality.The flat-plate bacterial colony of this bacterial strain is cultivated form and is seen Fig. 2.
(3) physio-biochemical characteristics
Fermentation :+; Nitrate salt :-; The urine enzyme :-; The blue B of diazo (DBB) experiment :-.Annotate: "+" expression positive reaction, "-" expression negative reaction.
Based on above characteristic; Bacterial strain LYMT-Y1 is accredited as yeast saccharomyces cerevisiae (Saccharomyces cerevisiae); And be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 2nd, 2009 and (be called for short CGMCC; The address is: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preservation registration number is CGMCC № 3382.
AFB in embodiment 2,3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 1
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, and 30 ℃, rotation radius 20mm is behind the 180rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration; Get an amount of fermented liquid according to count results; The centrifugal then yeast saccharomyces cerevisiae thalline that obtains; Above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0), and at 121 ℃ of heating 30min, centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was accomplished.
Pipette 2ml YPD substratum, detect through performance liquid chromatography and obtain AFB in this substratum
1Concentration be 23.11 ± 0.87ng/g, this substratum is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 1.7 * 10
10Individual yeast cell/ml is provided with 30 ℃ of adsorption temps, adsorption time 2h; Shaking speed 200rpm; After effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the substratum of detoxification with the mixing solutions of above-mentioned thalline and substratum; Detect AFB in the detoxification substratum through performance liquid chromatography
1Concentration be 1.31 ± 0.10ng/g, this thalline is to AFB
1Adsorption rate reach 94.32 ± 0.43%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple MVs.
Reference examples 1
Pipette 2ml YPD substratum same as described above, detect and obtain AFB in this substratum
1Concentration be 23.11 ± 0.87ng/g, this substratum is added in the blank centrifuge tube, 30 ℃ of operative temperatures are set; Action time 2h, shaking speed 200rpm, the effect finish after; With substratum 10; The centrifugal 10min of 000rpm collects upper solution, detects AFB in the substratum through performance liquid chromatography
1Concentration be 23.11 ± 0.87ng/g.
Experimental result shows, 3382 pairs of AFBs of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, when the thalline addition is 1.7 * 10
10Individual yeast cell/ml, 121 ℃ of heating of thalline warp 30min, 30 ℃ of adsorption temps, adsorption time 2h, during shaking speed 200rpm, this thalline is to AFB in the substratum
1Adsorption rate reach 94.32 ± 0.43%, can make AFB in the substratum
1Concentration be reduced to 1.31 ± 0.10ng/g from 23.11 ± 0.87ng/g.
AFB in embodiment 3,3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 2
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, 25 ℃ of cultivations, rotation radius 26mm is behind the 200rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration; Get an amount of fermented liquid according to count results; The centrifugal then yeast saccharomyces cerevisiae thalline that obtains; Above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0) at 121 ℃ of heating 30min, and centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was accomplished.
Pipette 2ml YPD substratum, detect through performance liquid chromatography and obtain AFB in this substratum
1Concentration be 19.95 ± 0.30ng/g, this substratum is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 2.5 * 10
9Individual yeast cell/ml is provided with 25 ℃ of adsorption temps, adsorption time 1h; Shaking speed 200rpm; After effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the substratum of detoxification with the mixing solutions of above-mentioned thalline and substratum; Detect AFB in the detoxification substratum through performance liquid chromatography
1Concentration be 3.76 ± 0.05ng/g, this thalline is to AFB
1Adsorption rate reach 81.16 ± 0.26%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple MVs.
Reference examples 2
Pipette 2ml YPD substratum same as described above, detect and obtain AFB in this substratum
1Concentration be 19.95 ± 0.30ng/g, this substratum is added in the blank centrifuge tube, 25 ℃ of operative temperatures are set; Action time 1h, shaking speed 200rpm, the effect finish after; With substratum 10; The centrifugal 10min of 000rpm collects upper solution, detects AFB in the substratum through performance liquid chromatography
1Concentration be 19.95 ± 0.30ng/g.
Experimental result shows, AFB in 3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, thalline through 121 ℃ the heating 30min, 25 ℃ of adsorption temps, adsorption time 1h, during shaking speed 200rpm, this thalline is to AFB
1Adsorption rate reach 81.16 ± 0.26%, can make AFB in the substratum
1Concentration be reduced to 3.76 ± 0.05ng/g from 19.95 ± 0.30ng/g.
After handling according to the method for the foregoing description 2-3 and Comparative Examples 1-2, AFB in the substratum
1Concentration all significantly reduced.AFB in 3382 pairs of substratum of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test show, when the thalline addition is 1.7 * 10
10Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 30 ℃, and adsorption time is 2h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB in the substratum
1Adsorption rate reach 94.32%, AFB
1Concentration be reduced to 1.31ng/g from 23.11ng/g; When the thalline addition is 2.5 * 10
9Individual/ml, the thalline processing mode is 121 ℃ of heating 30min, and adsorption temp is 25 ℃, and adsorption time is 1h, and when shaking speed was 200rpm, the thalline of yeast saccharomyces cerevisiae was to AFB
1Adsorption rate reach 81.16%, AFB
1Concentration be reduced to 3.76ng/g from 19.95ng/g.
AFB in embodiment 4,3382 pairs of peanut pastes of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorption test 1.
Yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC № 3382 is inoculated in the YPD liquid nutrient medium, and 25 ℃, shaking table rotation radius 20mm is after shaking speed is 180rpm shaking culture 33h.With blood counting chamber counting fermented liquid concentration; Get an amount of fermented liquid according to count results; The centrifugal then yeast saccharomyces cerevisiae thalline that obtains; Above-mentioned thalline is suspended in the phosphoric acid buffer (pH6.0) at 121 ℃ of heating 30min, and centrifugal removal phosphoric acid buffer (pH6.0) obtained the yeast saccharomyces cerevisiae thalline after heating was accomplished.
Take by weighing the 2g peanut paste, detect through performance liquid chromatography and obtain AFB in this peanut paste
1Concentration be 22.50 ± 1.31ng/g, this peanut paste is joined in the centrifuge tube that fills above-mentioned yeast saccharomyces cerevisiae thalline, the thalline addition is 2.5 * 10
9Individual yeast cell/ml is provided with 23 ℃ of adsorption temps, adsorption time 30min; Shaking speed 200rpm; After effect finished, 10, the centrifugal 10min collection of 000rpm upper solution can obtain the peanut paste of detoxification with the mixing solutions of above-mentioned thalline and peanut paste; Detect AFB in the detoxification peanut paste through performance liquid chromatography
1Concentration be 2.63 ± 0.30ng/g, this thalline is to AFB
1Adsorption rate reach 88.32 ± 1.31%.Three repetitions of every processing, the result value of above-mentioned acquisition are three multiple MVs.
Reference examples 3
Take by weighing 2g peanut paste same as described above, detect and obtain AFB in this peanut paste
1Concentration be 22.50 ± 1.31ng/g, this peanut paste is added in the blank centrifuge tube, 23 ℃ of operative temperatures are set; Action time 30min, shaking speed 200rpm, the effect finish after; With peanut paste 10; The centrifugal 10min of 000rpm collects upper solution, detects AFB in the peanut paste through performance liquid chromatography
1Concentration be 2.63 ± 0.30ng/g.
Experimental result shows, 3382 pairs of peanut paste AFBs of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1 CGMCC №
1Adsorptive power very strong, thalline through 121 ℃ the heating 30min, 23 ℃ of adsorption temps, adsorption time 30min, when shaking speed was 200rpm, this thalline was to AFB in the peanut paste
1Adsorption rate reach 88.32 ± 1.31%, can make AFB in the peanut paste
1Concentration be reduced to 2.63 ± 0.30ng/g from 22.50 ± 1.31ng/g.
Claims (11)
1. yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1, its preservation registration number is CGMCC № 3382.
2. the application of yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382 in the biotechnological formulation of preparation adsorption of aflatoxin B1.
3. method for preparing the biotechnological formulation of adsorption of aflatoxin B1; Be ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1CGMCC № 3382; The centrifugal supernatant of removing of its fermented liquid is obtained thalline, be the biotechnological formulation of adsorption of aflatoxin B1.
4. method according to claim 3; It is characterized in that: the consisting of of the fermention medium of said ferment wine brewing yeast (Saccharomyces cerevisiae) LYMT-Y1CGMCC № .3382: yeast soaks powder 1%; Peptone 2%, glucose 2%, all the other are water; Said percentage composition is the quality percentage composition; The pH value of said fermention medium is 7.0-7.2.
5. method according to claim 3 is characterized in that: said fermentation culture conditions is 25 ℃～30 ℃, and shaking speed is 180rpm～200rpm, rotation radius 20mm～26mm, shaking culture 30h～34h.
6. according to any described method among the claim 3-5, it is characterized in that: the thalline of said acquisition is also through 121 ℃ of heating.
7. method according to claim 6 is characterized in that: the step of said heating is that the thalline with centrifugal acquisition is suspended in the phosphoric acid buffer at 121 ℃ of heating 30min, centrifugal again acquisition yeast saccharomyces cerevisiae thalline again.
8. the adsorption of aflatoxin B of any described method preparation among the claim 3-7
1Biotechnological formulation.
9. the biotechnological formulation that arbitrary described method prepares among the claim 3-7 is at adsorption of aflatoxin B
1In application.
10. application according to claim 9 is characterized in that: said adsorption of aflatoxin B
1Temperature condition be 20-30 ℃; The time of said absorption is 30-120min.
11. application according to claim 10 is characterized in that: said adsorption of aflatoxin B
1Temperature condition be 23 ℃; The time of said absorption is 30min.
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Effective date of registration: 20220126 Address after: Room 320, floor 3, building 49, yard 2, Yuanmingyuan West Road, Haidian District, Beijing 100193 Patentee after: Beijing Zhongnong taste detection technology Co.,Ltd. Address before: 100193 No. 2 Old Summer Palace West Road, Beijing, Haidian District Patentee before: INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES |