CN101768620A - Method for producing biogas by micro-reaction thermal-explosion straw fermentation - Google Patents
Method for producing biogas by micro-reaction thermal-explosion straw fermentation Download PDFInfo
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- CN101768620A CN101768620A CN201010108974A CN201010108974A CN101768620A CN 101768620 A CN101768620 A CN 101768620A CN 201010108974 A CN201010108974 A CN 201010108974A CN 201010108974 A CN201010108974 A CN 201010108974A CN 101768620 A CN101768620 A CN 101768620A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention discloses a method for producing biogas by micro-reaction thermal-explosion straw fermentation. The farm product straws and the wastes from forest and wood processing are used as the raw material. The raw material is firstly pretreated for micro-reaction thermal-explosion by attenuate sulfur trioxide gas and then fermented in anaerobic condition to produce biogas; the pretreated straws, methanogenic bacteria or (and) pseudomonas aeruginosa are fermented in anaerobic condition for 7-14 days and under 40-50 DEG C in an anaerobic fermentation vat. The method guarantees high usage of straw fibrin, tremendous biogas production, and no residue except for biogas and biogas slurry, effectively solving the problem that fibrin encrusts to form residues.
Description
One, technical field
The present invention relates to utilize the method for biomass fermentation methane productions such as straw, specifically a kind of method of micro-reaction thermal-explosion straw fermentation methane production.
Two, background technology
Waste (stalk, rice husk, corn cob, bagasse), mountain forest weeds, forest and converted products, waste paper and sawdust etc. after farm crop are used all can be used as lignocellulosic material, carry out Chemicals such as follow-up fermentation system ethanol, butanols, butyric acid and lactic acid thereby produce sugar by the biological process hydrolysis.At present, people's attention mainly concentrates on the exploitation of bioenergies such as cellulosic ethanol, uses trees, stalk and the contained cellulose raw producing and ethanol fuel of other plants, and the greenhouse gases of release are compared with gasoline can reduce 82%～85%.
In fact, the biogas that biomass such as stalk produce through anaerobically fermenting is a kind of more energy of cleaning, it can realize the high-quality utilization [renewable energy source of biomass resources such as stalk under the situation of small-scale and less capital contribution, 2008,26 (1): 50～54], be particularly suitable for rural area and samll cities and towns demand to clean energy.But; because of the protection that has xylogen in the stalk and the material that contains microorganism toxicity make directly is fermentation raw material when producing biogas with the stalk, and Mierocrystalline cellulose wherein is utilized hardly, and gas producing efficiency is low; and discharging difficulty behind the aerogenesis, even cause methane-generating pit to stop aerogenesis.
Yan Zhiying etc. [Sichuan Agricultural University's journal, 2009,27 (2): 176～179] adopt through 4 days pretreated stalks of composite fungus agent stack retting and produce biogas, and cellulosic utilization ratio is only about 14%, so still do not solve the problem of discharging difficulty.[CN101338273 such as Ye Xiaomei, applying date 2008.08.15] utilize the stalk fermentation after the heap corruption to produce biogas, and solved the problem of matter stream and exchange of substance difficulty in the fermentation of stalk dry method, but the deslagging difficult problem behind this solution wet type producing methane through anaerobic fermentation more not by natural pond liquid return-flow system.
People [WO/2008/044929A1] such as Van Groenestijn have invented the method that employing high temperature (150-220 ℃) boiling lignocellulose stalk prepares biogas, this method has improved cellulosic utilization ratio, but adopts the pyritous method still to need to consume lot of energy in its process.
People such as Zheng Zheng [CN101519668] have invented the method for utilizing preparing marsh gas by fermenting steam-exploded wood fiber straws, this method has been improved lignocellulose stalk anaerobic fermentation STRUCTURE DEPRESSION problem, the utilization ratio of stalk cellulose increases substantially, methane content is up to 65%～75% in the biogas, solved a deslagging difficult problem of producing behind the biogas.This method is packed into after with straw chopping, and to carry out vapour in the quick-fried equipment of vapour quick-fried, obtains Powdered and thread steam puffed stalk material, again the steam puffed stalk material carried out anaerobic fermentation in anaerobic biological reactor and go out biogas.Song Yongmin and Chen Hongzhang [environmental engineering journal, 2008,11 (2): 1564-1570] utilize cellulase degradation to separate through the quick-fried pretreated stalk of vapour, and methane is produced in fermentation then, and gas production rate reaches 153.0mL/g, are 2.9 times of steam puffed stalk not.But this method is subjected to the restriction of conditions such as high pressure steam and high-tension apparatus equally.
Three, summary of the invention
The present invention is directed to the defective of deslagging difficulty in the straw fermentation methane production method, aim to provide a kind of novel method of utilizing straw and mountain forest abandoned biomass fermentation methane production, technical problem to be solved is to prevent that the straw fermentation methane production from generating the natural pond slag.
Solution to this problem is that biomass such as straw are carried out pre-treatment and selected suitable anaerobically fermenting condition.Described pre-treatment is exactly with biomass such as micro-reaction thermal-explosion method processing straws, particular content is: diffuse to the radical reaction and the heat release in position of stalk inside and xylogen, Mierocrystalline cellulose and hemicellulose etc. with the sulfur trioxide gas of dilution, by the power of combining closely and the wherein cellulosic crystalline texture between this micro-reaction thermal-explosion destruction xylogen and the Mierocrystalline cellulose, eliminated of the inhibition of toxic substances such as xylogen to stalk and mountain forest abandoned biomass producing methane through anaerobic fermentation process, the utilization ratio height of stalk cellulose, gas production rate is big, does not almost have natural pond slag discharging.This method applicant has applied for Chinese invention patent, application number: 200910116615.X.
Technical scheme of the present invention is a raw material with crop stalk and forestry and wood working waste (being called for short straw down), the micro-reaction thermal-explosion pre-treatment and the anaerobically fermenting that comprise straw, described anaerobically fermenting be exactly pretreated straw and methanogen in the anaerobically fermenting pond in 40～50 ℃ of bottom fermentations 14～15 days, the inoculum size of methanogen is 10～20% (v/m) of straw.
Preferably: pretreated straw is the inoculation Trichoderma earlier, and solid-state aerobic fermentation inoculated methanogen after 7 days under the normal temperature, in 40～50 ℃ of following anaerobically fermentings 8～10 days, and respectively do for oneself 10～20% (v/m) of straw of the inoculum size of Trichoderma and methanogen.
Best: straw is inoculated Trichoderma earlier after the pre-treatment, solid-state aerobic fermentation inoculates methanogen and Pseudomonas aeruginosa in 40～50 ℃ of following anaerobism bottom fermentations 7～8 days under the normal temperature after 7 days, respectively do for oneself 10～20% (v/m) of straw of the inoculum size of Trichoderma, methanogen and Pseudomonas aeruginosa.
The effect of Trichoderma is to be that fermenting substrate generates cellulase and then enzymolysis Mierocrystalline cellulose with pretreated straw.The effect of Pseudomonas aeruginosa is the aldehydes matter in the degraded straw.
Also can directly add cellulase and replace Trichoderma, but cellulase to add earlier, and under 40～50 ℃, enzymolysis 15～20 hours, and then add methanogen or/and Pseudomonas aeruginosa in 40～50 ℃ of following anaerobically fermentings 7～8 days.
The alleged stalk of the present invention is agricultural crop straws such as rice straw, wheat straw stalk, maize straw, rape stalk, sweet potato rattan and soybean stalk, and the biomass of lignocellulose-containings such as mountain forest weeds, weedtree and wood working waste.
Handle straw with the sulfur trioxide gas thermal explosion among the present invention, the cellulosic weave construction of not only having loosened effectively, also eliminated simultaneously the hazardous and noxious substances that suppresses anerobe, the straw fiber element is fully utilized, not only gas production rate is big, and solved the difficult problem of Mierocrystalline cellulose crust slagging effectively, and have only natural pond liquid, almost there is not a natural pond slag.
Four, embodiment
Non-limiting examples is described below:
Embodiment one: rice straw cuts into 3-4cm, feeds the 3%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 1%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours; After the separation, washing, dry for standby.In the 1L anaerobic fermentation tank, add pretreated stalk 100g, mud 20g, methanogen inoculum density are 10% (v/m), add less water, sealing, and 45 ± 5 ℃ of following anaerobically fermentings 14 days, methane production is 51-67mL/g TS.
Embodiment two: the wheat straw stalk cuts into 3-4cm, feeds the 10%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 2%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours; After the separation, washing, dry for standby.In the 1L anaerobic fermentation tank, add pretreated stalk 100g, the Trichoderma inoculum size is that (spore suspension is 10 to 10mL
7Individual/mL), normal temperature oxygen consumption fermentation culture is after 7 days, adds mud 20g, adds pretreated rice straw 50g again, and methanogen inoculum density 10% (v/m) adds less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 8 days, methane production is 104-120mL/g TS.
Embodiment three: soybean stalk cuts into 3-4cm, feeds the 10%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 2%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours; After the separation, washing, dry for standby.In the 1L anaerobic fermentation tank, add pretreated stalk 100g, add Mierocrystalline cellulose crude enzyme liquid 20FIU (filter paper enzyme activity)/g (every gram substrate), 45 ± 5 ℃ of hydrolysis are after 18 hours, add mud 20g, add pretreated rice straw 50g again, insert Pseudomonas aeruginosa 10% (the OD value is 2), methanogen inoculum density 10% (v/m), add less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 7 days, methane production is 126-134mL/g TS.
Embodiment four: the sweet potato rattan cuts into 3-4cm, feeds the 15%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 2%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours; After the separation, washing, dry for standby.In the 1L anaerobic fermentation tank, add pretreated stalk 100g, the Trichoderma inoculum size is that (spore suspension is 10 to 10mL
7Individual/mL), normal temperature oxygen consumption fermentation culture is after 7 days, add mud 20g, add pretreated rice straw 50g again, insert Pseudomonas aeruginosa 10% (the OD value is 2), methanogen inoculum density 10% (v/m), add less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 7 days, methane production is 112-126mL/g TS.
Embodiment five: branch or timber scrap stock shearing-crushing become the particle of particle diameter≤0.5cm, feed the 15%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, 50 ℃ of insulations of water-bath 1 hour, add 2%NaOH, solid-to-liquid ratio 1: 10 was handled 4 hours, after the separation, washing, dry for standby.Select the 10L anaerobic fermentation tank for use, add pretreated biomass 500g, the Trichoderma inoculum size is that (the robe suspension is 10 to 50mL
7Individual/mL), normal temperature oxygen consumption fermentation culture is after 7 days, add mud 100g, add pretreated biomass 250g again, insert Pseudomonas aeruginosa 10% (the OD value is 2), methanogen inoculum density 10% (v/m), add less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 7 days, methane production is 140mL/g TS.
Embodiment six: maize straw cuts into≤the 0.3cm particle, feeds the 15%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 1%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours, after the separation, and washing, dry for standby.Select the 10L anaerobic fermentation tank for use, add pretreated stalk 700g, the Trichoderma inoculum size is that (spore suspension is 10 to 70mL
7Individual/mL), normal temperature oxygen consumption fermentation culture is after 7 days, add mud 150g, add pretreated stalk 350g again, insert Pseudomonas aeruginosa 10% (the OD value is 2), methanogen inoculum density 10% (v/m), add less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 7 days, methane production is 184mL/g TS.
Embodiment seven: rape stalk cuts into≤the 0.5cm particle, feeds the 5%v/v sulphur trioxide to pressure of the inside of a bottle≤0.1MPa, and 50 ℃ of insulations of water-bath 1 hour add 1%NaOH, and solid-to-liquid ratio 1: 10 was handled 4 hours, after the separation, and washing, dry for standby.Select the 10L anaerobic fermentation tank for use, add pretreated stalk 700g, the Trichoderma inoculum size is that (spore suspension is 10 to 70mL
7Individual/mL), normal temperature oxygen consumption fermentation culture is after 7 days, add mud 135g, add pretreated stalk 350g again, insert Pseudomonas aeruginosa 10% (the OD value is 2), methanogen inoculum density 10% (v/m), add less water, sealing, 45 ± 5 ℃ of following anaerobically fermentings 7 days, methane production is 174mL/g TS.
Claims (3)
1. the method for a micro-reaction thermal-explosion straw fermentation methane production, with crop stalk and forestry and wood working waste is raw material, the micro-reaction thermal-explosion pre-treatment and the anaerobically fermenting that comprise raw material, it is characterized in that: described anaerobically fermenting be pretreated straw and methanogen in the anaerobically fermenting pond in 40～50 ℃ of bottom fermentations 14～15 days, the inoculum size of methanogen is 10～20% of a straw.
2. method according to claim 1, it is characterized in that: pretreated straw is the inoculation Trichoderma earlier, and under the normal temperature, solid-state aerobic fermentation inoculated methanogen after 7 days, in 40～50 ℃ of anaerobically fermentings 8～10 days, the inoculum size of Trichoderma and methanogen 10～20% of the straw of respectively doing for oneself.
3. method according to claim 1 and 2, it is characterized in that: pretreated straw is the inoculation Trichoderma earlier, solid-state aerobic fermentation inoculates methanogen and Pseudomonas aeruginosa in 40～50 ℃ of following anaerobically fermentings 7～8 days under the normal temperature after 7 days, the inoculum size of Trichoderma, methanogen and Pseudomonas aeruginosa 10～20% of the straw of respectively doing for oneself.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154375A (en) * | 2011-01-26 | 2011-08-17 | 清华大学 | Method for transforming straws into methane by adopting microbiological method |
| CN104313058A (en) * | 2014-10-17 | 2015-01-28 | 苏州新协力环保科技有限公司 | Marsh gas power generation method using biomass energy sources |
| CN112795596A (en) * | 2021-01-29 | 2021-05-14 | 生帆 | Method for preparing hydrocarbon compound by using biomass material |
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2010
- 2010-02-05 CN CN201010108974A patent/CN101768620A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154375A (en) * | 2011-01-26 | 2011-08-17 | 清华大学 | Method for transforming straws into methane by adopting microbiological method |
| CN102154375B (en) * | 2011-01-26 | 2013-07-03 | 清华大学 | Method for transforming straws into methane by adopting microbe method |
| CN104313058A (en) * | 2014-10-17 | 2015-01-28 | 苏州新协力环保科技有限公司 | Marsh gas power generation method using biomass energy sources |
| CN112795596A (en) * | 2021-01-29 | 2021-05-14 | 生帆 | Method for preparing hydrocarbon compound by using biomass material |
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Open date: 20100707 |