CN101766189A - Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber - Google Patents
Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber Download PDFInfo
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- CN101766189A CN101766189A CN200810230338A CN200810230338A CN101766189A CN 101766189 A CN101766189 A CN 101766189A CN 200810230338 A CN200810230338 A CN 200810230338A CN 200810230338 A CN200810230338 A CN 200810230338A CN 101766189 A CN101766189 A CN 101766189A
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Abstract
The invention relates to an endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber, which is prepared by bacillus subtilis. The technical scheme adopted by the endophytic bacterial agent is as follows: mother solution is prepared by 0.7 to 2.0% of peptone, 0.3 to 1.0% of yeast extract, 0.5 to 1.0% of NaCl and the rest percent of water; then, the mother solution with the pH value of 5 to 9 is cultivated for 24 to 72 hours in oscillating and fermenting manners at the speed of 120 to 200r/min under the fermentation temperature of 20 to 32 DEG C according to the liquid volume of 25 to 55% and the inoculum size of 2 to 10%.
Description
Technical field:
(Bacillus subtilis, preservation date: on 09 04th, 2008, depositary institution's full name: China Committee for Culture Collection of Microorganisms common micro-organisms center, depositary institution are called for short: CGMCC, deposit number: CGMCC No.2659), and make microbial inoculum to the present invention relates to a kind of bacillus subtilis.
Background technology:
Cucumber fusarium axysporum (Fusarium oxysporum f.sp.cucumerinum) is to endanger one of severe diseases the most during cucumber produces, and extensively betides in the protection ground.The production loss 10%-30% that cause because of cucumber fusarium axysporum every year, the serious time is up to more than 50%.This sick major measure of control is chemical control and grafting at present, because some producer uses chemical pesticide for a long time, blindly go and buy Chinese medicine, strengthen the agricultural chemicals usage amount arbitrarily, arbitrarily increase the agricultural chemicals access times, What is more uses high poison, persistent pesticide, causes this disease pesticide resistance to strengthen, residue of pesticide increase the weight of, the harm consumer health.Though grafting can reduce the incidence of disease, labour intensity strengthens, and production cost increases.
Endophytic bacterium (endophytic bacteria) be meant can be in the health plant tissue perch and plant is not caused substantive harm, and set up harmonious microorganism of uniting relation with plant.Endogenetic bacteria has stable living space in plant corpus, be not subject to the influence of external environmental condition, can independent breeding and transmission in plant corpus.It has avoided the influence of unfavorable factors such as temperature, ultraviolet ray, nutrition poorness, it is abundant to have comparatively safe stable living environment, source of nutrition, thereby be more conducive to its performance biological control potential, discover that the part endogenetic bacteria has the promotion plant growing or suppresses the effect that the pathogenic bacteria growing reaches the control disease.Endogenetic bacteria is used for biological control at present, has caused extensive concern in the world.Have about separation screening in various plants bodies such as cotton, potato, paddy rice, capsicum, cauliflower, tobacco to endogenetic bacteria both at home and abroad with prophylaxis effect.
Bacillus subtilis is the endogenetic bacteria of a lot of plants, and existing numerous species is widely used in the biological control of plant disease.Utilize tobacco plant endophytic Bacillus subtilis control tobacco bacterial wilt, field efficacy 43%-78%.The endophytic Bacillus subtilis bacterial strain B47 that separates from the tomato stem reaches 79.79% to the control efficiency of bacterial wilt of tomato.Utilize peanut endophytic Bacillus subtilis control peanut damping off and fusarium wilt effect obvious.The endophytic Bacillus subtilis that separates from balloonflower root has certain inhibitory action to the balloonflower root damping off.
Summary of the invention:
The present invention is exactly at the problems referred to above, and a kind of plant endogenesis microbial inoculum that is used to promote cucumber growth, control cucumber fusarium axysporum is provided.
The present invention adopts following technical scheme: peptone 0.7～2.0%, yeast extract 0.3～1.0%, NaCl0.5～1.0%, and all the other are water, are made into mother liquor; Is 25～55% with mother liquor by liquid amount, 20～32 ℃ of fermentation temperatures, inoculum concentration 2～10%, pH value 5～9; 120～200r/min oscillation and fermentation cultivation got final product in 24-72 hour.
Beneficial effect of the present invention:
Endophyte of plant of the present invention derives from the cucumber plant body, so all be safe to plant itself and people, people and plant is developed immunity to drugs, and also can as a part of agricultural chemicals hypertoxicity not arranged, and has no side effect in its production, process.
Endophyte of plant of the present invention is to have competition and two kinds of functions of antagonism concurrently, can promote cucumber growth, and prevention and treatment cucumber fusarium axysporum have good control efficiency.In the control test of cucumber fusarium axysporum, plant endogenesis microbial inoculum control efficiency of the present invention has reached 65.7%, and is suitable with 800 times of preventive effects 68.6% of 50% carbendazim WP.Have a extensive future.
Classification name: bacillus subtilis (Bacillus subtilis)
Microorganism (strain): B504
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center
The Datun Road, Chaoyang District, Beijing City, DSMZ of Institute of Microorganism, Academia Sinica
Postcode: 100101
The preservation time: on September 4th, 2008
Deposit number: CGMCC No:2659
Embodiment:
Strain preparation
Bacillus subtilis B504 bacterial strain, by LB medium activation culture, LB medium component: yeast extract 5g, peptone 10g, agar 15g, NaCl 10g, distilled water 1000ml, pH value 7.2,28～31 ℃ of temperature.
Embodiment 1:
The plant endogenesis microbial inoculum promotes cucumber radicle and plant strain growth test
Plant endogenesis microbial inoculum of the present invention is peptone 1.0%, yeast extract 0.5%, NaCl 0.7%, all the other are water, is made into mother liquor, is 25% with mother liquor by liquid amount, 29 ℃ of fermentation temperatures, inoculum concentration 6%, pH value 7～8; 140r/min oscillation and fermentation cultivation 46 hours.Dilute about 1000 times, be used for vernalization.
1. the plant endogenesis microbial inoculum promotes the radicle growth situation
Receive with 3% hypochlorous acid and to carry out seed disinfection 15min, clear water washes repeatedly, is placed in the clean culture dish that is lined with filter paper, and every ware is put about 50 in seed, and each handles 3 wares, adds in each culture dish about bacterium liquid 15ml, adds seed then.Culture dish is put into 25 ℃ of constant incubators.During sowing preceding (radicle is about about 9mm), downcut radicle, get 100 at random and weigh.The results are shown in Table 1.
Table 1 plant endogenesis microbial inoculum promotes the radicle growth situation
2. the plant endogenesis microbial inoculum promotes the plant strain growth situation
Above-mentioned test residue seed is sowed in the plastic crate that natural soils are housed.Measure plant weight after 30 days, each processing 30 strains of weighing are calculated average plant height, plant fresh weight, plant root fresh weight and be the results are shown in Table 2.
Table 2B504 bacterial strain promotes the plant strain growth situation
Find out that from the result of the test of table 1, table 2 plant endogenesis microbial inoculum of the present invention has the effect that promotes cucumber seeds radicle and plant strain growth.
Embodiment 2:
The test of plant endogenesis microbial inoculum control cucumber fusarium axysporum
Plant endogenesis microbial inoculum of the present invention is peptone 1.0%, yeast extract 0.5%, NaCl 0.7%, all the other are water, is made into mother liquor, is 25% with mother liquor by liquid amount, 29 ℃ of fermentation temperatures, inoculum concentration 6%, pH value 7～8; 140r/min oscillation and fermentation cultivation 46 hours.
The plant endogenesis microbial inoculum of preparation is carried out the cucumber fusarium axysporum test.Find out that from the result of the test of table 3 plant endogenesis microbial inoculum of the present invention has the better prevention effect to cucumber fusarium axysporum.1000 times of control efficiency of plant endogenesis microbial inoculum wherein of the present invention are good, reach 65.7%, and are suitable with 50% carbendazim WP preventive effect 68.6%.
The effect analysis of table 3 plant endogenesis microbial inoculum control cucumber fusarium axysporum
Claims (2)
1. the plant endogenesis microbial inoculum that can promote cucumber growth and control cucumber fusarium axysporum is characterized in that all the other are water, are made into mother liquor with peptone 0.7～2.0%, yeast extract 0.3～1.0%, NaCl 0.5～1.0%; Is 25～55% with mother liquor by liquid amount, 20～32 ℃ of fermentation temperatures, inoculum concentration 2～10%, pH value 5～9; 120～200r/min oscillation and fermentation cultivation got final product in 24-72 hour.
2. a kind of plant endogenesis microbial inoculum that can promote cucumber growth and control cucumber fusarium axysporum according to claim 1 is characterized in that all the other are water, are made into mother liquor with peptone 1.0%, yeast extract 0.5%, NaCl 0.7%; Is 25% with mother liquor by liquid amount, 29 ℃ of fermentation temperatures, and inoculum concentration 6%, pH value 7～8,140r/min oscillation and fermentation cultivation 46 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102303380A CN101766189B (en) | 2008-12-29 | 2008-12-29 | Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008102303380A CN101766189B (en) | 2008-12-29 | 2008-12-29 | Endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber |
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| CN101766189A true CN101766189A (en) | 2010-07-07 |
| CN101766189B CN101766189B (en) | 2012-01-25 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586136A (en) * | 2012-01-09 | 2012-07-18 | 天津市农业生物技术研究中心 | Microbial inoculant for improving disease resistance and promoting growth of plants |
| CN103374536A (en) * | 2012-04-20 | 2013-10-30 | 中国农业大学 | Bacillus subtilis and application thereof in prevention and treatment of fusarium wilt of cucumber |
| CN105475363A (en) * | 2015-12-01 | 2016-04-13 | 中国农业科学院农业资源与农业区划研究所 | Cucumber growth promotion composite microbial agent and application thereof |
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| CN105838656A (en) * | 2016-06-15 | 2016-08-10 | 东北农业大学 | Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus |
| CN106591157A (en) * | 2017-02-23 | 2017-04-26 | 北京市农林科学院 | Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites |
| CN111802406A (en) * | 2020-06-23 | 2020-10-23 | 秦皇岛禾苗生物技术有限公司 | Application of bacillus subtilis J-5 in preventing and treating cucumber fusarium wilt |
-
2008
- 2008-12-29 CN CN2008102303380A patent/CN101766189B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586136A (en) * | 2012-01-09 | 2012-07-18 | 天津市农业生物技术研究中心 | Microbial inoculant for improving disease resistance and promoting growth of plants |
| CN102586136B (en) * | 2012-01-09 | 2013-08-14 | 天津市农业生物技术研究中心 | Microbial inoculant for improving disease resistance and promoting growth of plants |
| CN103374536A (en) * | 2012-04-20 | 2013-10-30 | 中国农业大学 | Bacillus subtilis and application thereof in prevention and treatment of fusarium wilt of cucumber |
| CN103374536B (en) * | 2012-04-20 | 2015-02-11 | 中国农业大学 | Bacillus subtilis and application thereof in prevention and treatment of fusarium wilt of cucumber |
| CN105475363A (en) * | 2015-12-01 | 2016-04-13 | 中国农业科学院农业资源与农业区划研究所 | Cucumber growth promotion composite microbial agent and application thereof |
| CN105475363B (en) * | 2015-12-01 | 2018-01-16 | 中国农业科学院农业资源与农业区划研究所 | A kind of composite bacteria agent for promoting cucumber growth and its application |
| CN105779302A (en) * | 2016-03-24 | 2016-07-20 | 江西天人生态股份有限公司 | Hypocrea virens-containing composite microbial agent for controlling cucumber fusarium wilt, and preparation method of hypocrea virens-containing composite microbial agent |
| CN105838656A (en) * | 2016-06-15 | 2016-08-10 | 东北农业大学 | Broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of broad-spectrum disease resisting, growth promotion and stress resisting bacillus |
| CN106591157A (en) * | 2017-02-23 | 2017-04-26 | 北京市农林科学院 | Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites |
| CN111802406A (en) * | 2020-06-23 | 2020-10-23 | 秦皇岛禾苗生物技术有限公司 | Application of bacillus subtilis J-5 in preventing and treating cucumber fusarium wilt |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101766189B (en) | 2012-01-25 |
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